Rw6G025520

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0016564
Physical Location & Seq
Reverse (-)
48890015 .. 48891732
1718 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G025520.1

Sequence Viewer

Length: 1278 bp
ATGAACTGCACTTCCACTTCGCTGCTGAGCTCCTCCTTCCTCCCAACTCCACCTTTGAATCCCAACAGCTCCTCCTCCTCCTCCTCCTCCTCCTTCCCACGGGCCCCACCCAAGCCCCTCATCCGCCGCGTCACCACTAAAATGTCCCTCAACCAACAACCCCCCCAACCGCCGGACCCAACCTCCCTGCTCACCTCCGTCACCAAGCTCCTCTGGGGCCCTTCCCTCCCTCCCGGCCTCCTCATCTCCACCGTCCGCGCAGCCTGGAACACCACGTGGCGGCTCATGATGTCCCAGCTCGCCCCCTCCGATCCCTCCCTCGCCTACACCCGACCCCCTTCAAGGTTTCGAGCCACACAAATCCCCCGCCAAAACCAGACCTCCCTCCACCTCTACGTCGGCCTCCCCTGCCCCTGGGCCCACCGCACCCTCATCGTCCGAGCCCTCAAGGGCCTCGACGGTGCCGTCCCCGTCTCAATCGCCGCTCCGGGCACGGACGGCTCCTGGGAATTCAAGAACACCCGCGACCCGGATCCGGATACCCTCGACCCGACCCGGGACAAAGCTAACGGGTGCAGGACTCTGAAAGAGGTCTACAAATCAAGAGCCGGAGGGTACGACGGTCGGTCCACAGTGCCAATGCTATGGGACGCAAAGAACAAACAAGTACTATGCAACGAAAGCTACGATATAATCGAATTGTTCAATTCGGGTCTGAATGAACTGGCCCGTAACCCGGGTTTGGATCTTTCACCGCCTGCATTGAAGCCCAAGATTCAAGAATGGAACAGCATAATCTACCCCAATGTCAATAATGGAGTCTATAGGTGTGGATTTGCTCAGTCTCAACAAGCATATGATGTAGCAGTGAATGACTTGTTCAACACACTGGACAAGTTGGAAGATCATTTGAGCCGATTTCGCTACCTGTGCGGAGATGAACTGACGCTTGCCGATGTTTGCCTCTTCACCACTCTCATCCGGTTCGACCTCGCCTACAATGTCTTGTTCAAATGCACCAGGAGGAAGCTGATCGAGTATCCAAACCTCCATGCTTACATGCGTGACATTTACCAGATTCCAAAGGTTGCAGAAACTTGCAATTTTCAGTTGATCATGGAGGGCTACTACAAAACCCTTTTCCCACTCAATCCAGGCAGCATTCAGCCTATAATTCCTGCCGGATCCGAGCATCAAGAACTTCAGAGGCCGCATAACAGGGATTCATTGTCCCTTAAAGACGCCAGGGAATCAGCAGCAGCACTCTCTGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

425

Amino Acids

47.41

Weight (kDa)

8.95

Isoelectric Point (pI)

43.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 137 - 235 8.5e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 279 - 361 2.5e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 283 - 355 6e-17 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 288 - 365 2.9e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44000
fragaria_vesca FvH4_2g20660
prunus_persica Prupe.8G216400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0286841
rosa_laevigata RLG00000012532
rosa_multiflora Rmu_sc0011217.1_g000001 Rmu_sc0018293.1_g000001
rosa_roxburghii Rroxscaffold_7G00181880
rosa_rugosa Rorug06G0183900
rosa_samantha Rh6AG296000 Rh6BG300300 Rh6CG308200 Rh6DG292000
rosa_wichuraiana Rw6G025520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 461
AccBSI CCGCTC 1 cut(s) 485
AccI GTMKAC 1 cut(s) 594
AccII CGCG 3 cut(s) 129, 258, 525
AccIII TCCGGA 1 cut(s) 535
AclWI GGATC 6 cut(s) 305, 527, 540, 753, 1179, 1192
AcsI RAATTY 1 cut(s) 509
AcuI CTGAAG 1 cut(s) 1187
AcvI CACGTG 1 cut(s) 276
AcyI GRCGYC 1 cut(s) 1242
AdeI CACNNNGTG 1 cut(s) 276
AfaI GTAC 2 cut(s) 617, 669
AgsI TTSAA 8 cut(s) 58, 342, 514, 706, 766, 779, 883, 1012
AhdI GACNNNNNGTC 1 cut(s) 625
AjnI CCWGG 6 cut(s) 263, 413, 503, 1019, 1153, 1244
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 7 cut(s) 30, 69, 208, 298, 566, 684, 1030
AluI AGCT 7 cut(s) 30, 69, 208, 298, 566, 684, 1030
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 478, 849
AlwI GGATC 6 cut(s) 305, 527, 540, 753, 1179, 1192
Ama87I CYCGRG 2 cut(s) 555, 736
Aor13HI TCCGGA 1 cut(s) 535
AoxI GGCC 8 cut(s) 102, 217, 235, 400, 417, 451, 726, 1208
ApaI GGGCCC 3 cut(s) 106, 221, 421
ApeKI GCWGC 5 cut(s) 22, 260, 1158, 1256, 1259
ApoI RAATTY 1 cut(s) 509
AspLEI GCGC 1 cut(s) 260
AsuC2I CCSGG 7 cut(s) 234, 489, 530, 556, 557, 737, 738
AsuHPI GGTGA 5 cut(s) 124, 184, 193, 744, 961
AvaI CYCGRG 2 cut(s) 555, 736
AvaII GGWCC 2 cut(s) 175, 627
BaeGI GKGCMC 4 cut(s) 106, 221, 421, 494
BaeI ACNNNNGTAYC 2 cut(s) 607, 640
BamHI GGATCC 2 cut(s) 532, 1184
BanI GGYRCC 1 cut(s) 461
BanII GRGCYC 5 cut(s) 32, 106, 221, 421, 445
BbrPI CACGTG 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 5 cut(s) 9, 272, 1170, 1268, 1271
BceAI ACGGC 2 cut(s) 449, 514
BcgI CGANNNNNNTGC 2 cut(s) 415, 449
BciT130I CCWGG 6 cut(s) 265, 415, 505, 1021, 1155, 1246
BciVI GTATCC 2 cut(s) 532, 1050
BclI TGATCA 1 cut(s) 1113
BcnI CCSGG 7 cut(s) 234, 489, 530, 556, 557, 737, 738
BcoDI GTCTC 2 cut(s) 478, 849
BfmI CTRYAG 1 cut(s) 823
BfuI GTATCC 2 cut(s) 532, 1050
BisI GCNGC 9 cut(s) 23, 127, 261, 281, 483, 1159, 1211, 1257, 1260
BlpI GCTNAGC 1 cut(s) 26
BlsI GCNGC 9 cut(s) 24, 128, 262, 282, 484, 1160, 1212, 1258, 1261
BmcAI AGTACT 1 cut(s) 669
Bme18I GGWCC 2 cut(s) 175, 627
BmeRI GACNNNNNGTC 1 cut(s) 625
BmeT110I CYCGRG 2 cut(s) 555, 736
BmsI GCATC 1 cut(s) 1201
Bpu1102I GCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 431
BpuMI CCSGG 7 cut(s) 234, 489, 530, 556, 557, 737, 738
BsaAI YACGTR 1 cut(s) 276
BsaHI GRCGYC 1 cut(s) 1242
BsaJI CCNNGG 7 cut(s) 98, 413, 414, 504, 555, 736, 1245
BsaWI WCCGGW 2 cut(s) 535, 981
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 167, 197, 365, 395
Bse1I ACTGG 2 cut(s) 729, 894
BseAI TCCGGA 1 cut(s) 535
BseBI CCWGG 6 cut(s) 265, 415, 505, 1021, 1155, 1246
BseDI CCNNGG 7 cut(s) 98, 413, 414, 504, 555, 736, 1245
BseGI GGATG 2 cut(s) 120, 978
BseMII CTCAG 2 cut(s) 17, 854
BseNI ACTGG 2 cut(s) 729, 894
BseSI GKGCMC 4 cut(s) 106, 221, 421, 494
BseXI GCAGC 5 cut(s) 9, 272, 1170, 1268, 1271
BseYI CCCAGC 1 cut(s) 294
BsgI GTGCAG 1 cut(s) 595
Bsh1236I CGCG 3 cut(s) 129, 258, 525
Bsh1285I CGRYCG 1 cut(s) 625
BshFI GGCC 8 cut(s) 104, 219, 237, 402, 419, 453, 728, 1210
BshNI GGYRCC 1 cut(s) 461
BsiEI CGRYCG 1 cut(s) 625
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 555, 736
BslFI GGGAC 6 cut(s) 130, 277, 452, 572, 662, 1216
BsmAI GTCTC 2 cut(s) 478, 849
BsmBI CGTCTC 1 cut(s) 478
BsmFI GGGAC 6 cut(s) 130, 277, 452, 572, 662, 1216
BsmI GAATGC 1 cut(s) 1161
BsnI GGCC 8 cut(s) 104, 219, 237, 402, 419, 453, 728, 1210
BsoBI CYCGRG 2 cut(s) 555, 736
Bsp120I GGGCCC 3 cut(s) 102, 217, 417
Bsp1286I GDGCHC 6 cut(s) 32, 106, 221, 421, 445, 494
Bsp13I TCCGGA 1 cut(s) 535
Bsp143I GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
Bsp1720I GCTNAGC 1 cut(s) 26
BspANI GGCC 8 cut(s) 104, 219, 237, 402, 419, 453, 728, 1210
BspCNI CTCAG 2 cut(s) 18, 853
BspEI TCCGGA 1 cut(s) 535
BspFNI CGCG 3 cut(s) 129, 258, 525
BspHI TCATGA 1 cut(s) 285
BspPI GGATC 6 cut(s) 305, 527, 540, 753, 1179, 1192
BspT107I GGYRCC 1 cut(s) 461
BsrBI CCGCTC 1 cut(s) 485
BsrI ACTGG 2 cut(s) 729, 894
BssECI CCNNGG 7 cut(s) 98, 413, 414, 504, 555, 736, 1245
BssMI GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
BssNI GRCGYC 1 cut(s) 1242
Bst2UI CCWGG 6 cut(s) 265, 415, 505, 1021, 1155, 1246
Bst4CI ACNGT 4 cut(s) 253, 461, 623, 634
Bst6I CTCTTC 1 cut(s) 971
BstACI GRCGYC 1 cut(s) 1242
BstBAI YACGTR 1 cut(s) 276
BstC8I GCNNGC 3 cut(s) 300, 759, 951
BstDEI CTNAG 2 cut(s) 26, 840
BstDSI CCRYGG 1 cut(s) 98
BstF5I GGATG 2 cut(s) 120, 978
BstFNI CGCG 3 cut(s) 129, 258, 525
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 7 cut(s) 313, 535, 748, 907, 1035, 1116, 1187
BstMAI GTCTC 2 cut(s) 478, 849
BstMBI GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
BstMCI CGRYCG 1 cut(s) 625
BstMWI GCNNNNNNNGC 5 cut(s) 408, 498, 681, 921, 930
BstNI CCWGG 6 cut(s) 265, 415, 505, 1021, 1155, 1246
BstNSI RCATGY 1 cut(s) 1063
BstSFI CTRYAG 1 cut(s) 823
BstSLI GKGCMC 4 cut(s) 106, 221, 421, 494
BstUI CGCG 3 cut(s) 129, 258, 525
BstV1I GCAGC 5 cut(s) 9, 272, 1170, 1268, 1271
BstX2I RGATCY 3 cut(s) 532, 745, 1184
BstXI CCANNNNNNTGG 1 cut(s) 645
BstYI RGATCY 3 cut(s) 532, 745, 1184
BsuI GTATCC 2 cut(s) 532, 1050
BsuRI GGCC 8 cut(s) 104, 219, 237, 402, 419, 453, 728, 1210
BtgI CCRYGG 1 cut(s) 98
BtsCI GGATG 2 cut(s) 120, 978
BtsI GCAGTG 1 cut(s) 873
BtsIMutI CAGTG 3 cut(s) 639, 873, 887
Cac8I GCNNGC 3 cut(s) 300, 759, 951
CciI TCATGA 1 cut(s) 285
CfoI GCGC 1 cut(s) 260
Cfr9I CCCGGG 2 cut(s) 555, 736
CseI GACGC 4 cut(s) 118, 659, 955, 1250
Csp6I GTAC 2 cut(s) 616, 668
CviAII CATG 4 cut(s) 286, 1052, 1060, 1117
CviQI GTAC 2 cut(s) 616, 668
DdeI CTNAG 2 cut(s) 26, 840
DpnI GATC 7 cut(s) 312, 534, 747, 906, 1034, 1115, 1186
DpnII GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
DraIII CACNNNGTG 1 cut(s) 276
DrdI GACNNNNNNGTC 1 cut(s) 464
DriI GACNNNNNGTC 1 cut(s) 625
DseDI GACNNNNNNGTC 1 cut(s) 464
Eam1104I CTCTTC 1 cut(s) 971
Eam1105I GACNNNNNGTC 1 cut(s) 625
EarI CTCTTC 1 cut(s) 971
EciI GGCGGA 1 cut(s) 113
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 5 cut(s) 32, 106, 221, 421, 445
Eco47I GGWCC 2 cut(s) 175, 627
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 1187
Eco72I CACGTG 1 cut(s) 276
Eco88I CYCGRG 2 cut(s) 555, 736
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 4 cut(s) 103, 217, 218, 451
EcoRI GAATTC 1 cut(s) 509
EcoRII CCWGG 6 cut(s) 263, 413, 503, 1019, 1153, 1244
EcoT38I GRGCYC 5 cut(s) 32, 106, 221, 421, 445
Esp3I CGTCTC 1 cut(s) 478
FaeI CATG 4 cut(s) 289, 1055, 1063, 1120
FaqI GGGAC 6 cut(s) 130, 277, 452, 572, 662, 1216
FatI CATG 4 cut(s) 285, 1051, 1059, 1116
FauI CCCGC 2 cut(s) 374, 530
FauNDI CATATG 1 cut(s) 856
FbaI TGATCA 1 cut(s) 1113
FblI GTMKAC 1 cut(s) 594
Fnu4HI GCNGC 9 cut(s) 23, 127, 261, 281, 483, 1159, 1211, 1257, 1260
FokI GGATG 2 cut(s) 107, 965
FriOI GRGCYC 5 cut(s) 32, 106, 221, 421, 445
Fsp4HI GCNGC 9 cut(s) 23, 127, 261, 281, 483, 1159, 1211, 1257, 1260
GlaI GCGC 1 cut(s) 259
GluI GCNGC 9 cut(s) 23, 127, 261, 281, 483, 1159, 1211, 1257, 1260
GsaI CCCAGC 1 cut(s) 298
HaeIII GGCC 8 cut(s) 104, 219, 237, 402, 419, 453, 728, 1210
HgaI GACGC 4 cut(s) 118, 659, 955, 1250
HhaI GCGC 1 cut(s) 260
Hin1I GRCGYC 1 cut(s) 1242
Hin1II CATG 4 cut(s) 289, 1055, 1063, 1120
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HinfI GANTC 7 cut(s) 58, 580, 775, 819, 1078, 1223, 1250
HphI GGTGA 5 cut(s) 124, 184, 193, 744, 961
Hpy166II GTNNAC 2 cut(s) 595, 630
Hpy188I TCNGA 6 cut(s) 310, 440, 585, 717, 1189, 1206
Hpy188III TCNNGA 6 cut(s) 286, 514, 536, 603, 779, 1196
Hpy8I GTNNAC 2 cut(s) 595, 630
Hpy99I CGWCG 3 cut(s) 401, 461, 623
HpyAV CCTTC 4 cut(s) 46, 103, 231, 348
HpyCH4III ACNGT 4 cut(s) 253, 461, 623, 634
HpyCH4IV ACGT 2 cut(s) 275, 396
HpyCH4V TGCA 7 cut(s) 9, 576, 675, 761, 1017, 1091, 1101
HpyF10VI GCNNNNNNNGC 5 cut(s) 408, 498, 681, 921, 930
HpyF3I CTNAG 2 cut(s) 26, 840
HpySE526I ACGT 2 cut(s) 275, 396
Hsp92I GRCGYC 1 cut(s) 1242
Hsp92II CATG 4 cut(s) 289, 1055, 1063, 1120
HspAI GCGC 1 cut(s) 258
Kpn2I TCCGGA 1 cut(s) 535
Ksp22I TGATCA 1 cut(s) 1113
Kzo9I GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
LmnI GCTCC 5 cut(s) 35, 74, 213, 490, 506
Lsp1109I GCAGC 5 cut(s) 9, 272, 1170, 1268, 1271
LweI GCATC 1 cut(s) 1201
MaeII ACGT 2 cut(s) 275, 396
MaeIII GTNAC 4 cut(s) 130, 199, 731, 1064
MalI GATC 7 cut(s) 312, 534, 747, 906, 1034, 1115, 1186
MbiI CCGCTC 1 cut(s) 485
MboI GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
MboII GAAGA 2 cut(s) 914, 958
MflI RGATCY 3 cut(s) 532, 745, 1184
MhlI GDGCHC 6 cut(s) 32, 106, 221, 421, 445, 494
MluCI AATT 5 cut(s) 509, 698, 706, 1102, 1173
MlyI GAGTC 2 cut(s) 574, 828
MmeI TCCRAC 1 cut(s) 879
MroI TCCGGA 1 cut(s) 535
MseI TTAA 2 cut(s) 1236, 1276
MslI CAYNNNNRTG 1 cut(s) 140
Mva1269I GAATGC 1 cut(s) 1161
MvaI CCWGG 6 cut(s) 265, 415, 505, 1021, 1155, 1246
MvnI CGCG 3 cut(s) 129, 258, 525
MwoI GCNNNNNNNGC 5 cut(s) 408, 498, 681, 921, 930
NciI CCSGG 7 cut(s) 234, 489, 530, 556, 557, 737, 738
NdeI CATATG 1 cut(s) 856
NdeII GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
NlaIII CATG 4 cut(s) 289, 1055, 1063, 1120
NmuCI GTSAC 3 cut(s) 130, 199, 1064
NspI RCATGY 1 cut(s) 1063
PagI TCATGA 1 cut(s) 285
PasI CCCWGGG 1 cut(s) 414
PcsI WCGNNNNNNNCGW 4 cut(s) 306, 462, 684, 693
PctI GAATGC 1 cut(s) 1161
PfeI GAWTC 5 cut(s) 58, 775, 1078, 1223, 1250
PkrI GCNGC 9 cut(s) 24, 128, 262, 282, 484, 1160, 1212, 1258, 1261
PleI GAGTC 2 cut(s) 574, 827
PmaCI CACGTG 1 cut(s) 276
PmlI CACGTG 1 cut(s) 276
PpsI GAGTC 2 cut(s) 574, 827
Ppu21I YACGTR 1 cut(s) 276
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 6 cut(s) 263, 413, 503, 1019, 1153, 1244
PspCI CACGTG 1 cut(s) 276
PspFI CCCAGC 1 cut(s) 294
PspGI CCWGG 6 cut(s) 263, 413, 503, 1019, 1153, 1244
PspOMI GGGCCC 3 cut(s) 102, 217, 417
PsuI RGATCY 3 cut(s) 532, 745, 1184
RsaI GTAC 2 cut(s) 617, 669
RsaNI GTAC 2 cut(s) 616, 668
RseI CAYNNNNRTG 1 cut(s) 140
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 1236, 1276
SatI GCNGC 9 cut(s) 23, 127, 261, 281, 483, 1159, 1211, 1257, 1260
Sau3AI GATC 7 cut(s) 310, 532, 745, 904, 1032, 1113, 1184
ScaI AGTACT 1 cut(s) 669
SchI GAGTC 2 cut(s) 574, 828
SduI GDGCHC 6 cut(s) 32, 106, 221, 421, 445, 494
SfaNI GCATC 1 cut(s) 1201
SfcI CTRYAG 1 cut(s) 823
SinI GGWCC 2 cut(s) 175, 627
SmaI CCCGGG 2 cut(s) 557, 738
SmiMI CAYNNNNRTG 1 cut(s) 140
SmlI CTYRAG 1 cut(s) 446
SmoI CTYRAG 1 cut(s) 446
Sse9I AATT 5 cut(s) 509, 698, 706, 1102, 1173
SstI GAGCTC 1 cut(s) 32
TaaI ACNGT 4 cut(s) 253, 461, 623, 634
TaiI ACGT 2 cut(s) 278, 399
TaqI TCGA 6 cut(s) 349, 456, 546, 696, 987, 1035
TaqII GACCGA 1 cut(s) 615
TasI AATT 5 cut(s) 509, 698, 706, 1102, 1173
TatI WGTACW 1 cut(s) 667
TauI GCSGC 4 cut(s) 129, 283, 485, 1213
TfiI GAWTC 5 cut(s) 58, 775, 1078, 1223, 1250
Tru1I TTAA 2 cut(s) 1236, 1276
Tru9I TTAA 2 cut(s) 1236, 1276
TscAI CASTG 3 cut(s) 639, 873, 894
TseFI GTSAC 3 cut(s) 130, 199, 1064
TseI GCWGC 5 cut(s) 22, 260, 1158, 1256, 1259
Tsp45I GTSAC 3 cut(s) 130, 199, 1064
TspDTI ATGAA 4 cut(s) 17, 735, 954, 1215
TspGWI ACGGA 2 cut(s) 187, 509
TspMI CCCGGG 2 cut(s) 555, 736
TspRI CASTG 3 cut(s) 639, 873, 894
VpaK11BI GGWCC 2 cut(s) 175, 627
XapI RAATTY 1 cut(s) 509
XceI RCATGY 1 cut(s) 1063
XmaI CCCGGG 2 cut(s) 555, 736
XmiI GTMKAC 1 cut(s) 594
ZrmI AGTACT 1 cut(s) 669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.