RLG00000012532

Glutathione S-transferase omega-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0016564
Physical Location & Seq
Forward (+)
19653800 .. 19655248
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000012532

Sequence Viewer

Length: 1275 bp
ATGAACTGCACTTCCAGTTCGCTGCTGAGCTCCTCCTTCCTCCCAACTCCACCATTGAATCCCAACAGCTCCTCCTCCTCCTCCTTCCCACGGGCCCCACCCAAGCCCATCATCCGCCGCGTCACCACTAAAATGTCCCTCAACCAACAACCCCCCCAACCGCCGGACCCAACCTCCCTGCTCACCTCCGTCACCAAGCTCCTGTGGGGCCCATCCCTCCCTCCCGGCCTCCTCATCTCCACCGTCCGCGCAGCCTGGAACACCACGTGGCGGCTCATGATGTCCCAGCTCGCCCCCTCCGATCCCTCCCTCGCCTACACCCGACCCCCCTCCAGGTTTCGAGCCACACAAATCCCCCGCCAAAACCAGACCTCCCTCCACCTCTACGTGGGCCTCCCCTGCCCCTGGGCCCACCGCACCCTCATCGTCCGAGCCCTCAAGGGCCTCGACGACGCCGTCCCCGTCTCAATCGCCGCTCCGGGCACGGACGGCTCCTGGGAATTCAAGAACACCCGCGACCCGGATCCGGATACCCTCGACCCGACCCGGGACAAAGCAAACGGGTGCAGGACTCTGAAAGAGGTCTACAAATCAAGAGCGGGAGGGTACGACGGTCGGTCCACAGTGCCAATGCTATGGGATGCAAAGAACAAACAAGTACTATGCAACGAAAGCTACGATATAATCGAATTGTTCAATTCGGGTCTGAATGAACTGGCCCGTAACCCGGGTTTGGATCTTTCACCGCCTGCATTGAAGCCAAAGATTCAAGAATGGACCAGCATAATCTACCCCAATGTCAATAATGGAGTCTATAGGTGTGGATTTGCTCAGTCTCAACAAGCATATGATGTAGCAGTGAATGACCTGTTCAACACACTGGACAAGCTGGAAGATCATTTGAGCCGATTTCGCTACCTGTGCGGAGATGAACTGACGCTTGCGGATGTTTGCCTCTTCACCACTCTCATCCGGTTCGATCTCGCCTACAATGTCTTGTTCAAATGCACCAGGAGGAAGCTGATCGAGTATCCAAACCTTCATGCTTACATGCGTGACATTTACCAGATTCCAAAGGTTGCAGAAACTTGCAATTTTCAGTTGATCATGGAAGGCTACTACAAAACCCTTTTCCCACTCAACCCAGGCAGCATTCAGCCTATAATTCCTGCCGGATCCGAGCATCAAGAACTTCAGAGGCCGCATAACAGGGACTCATTGTCCCTTAAAGACGCCAGCGAATCAGCAGCAGCAGCAGCACTTTCTGTTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GST_C_2 GST_N_2

Protein Analysis

425

Amino Acids

47.25

Weight (kDa)

8.75

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N_2 PF13409 134 - 232 1.5e-09 Glutathione S-transferase, N-terminal domain
GST_C PF00043 276 - 358 2.3e-09 Glutathione S-transferase, C-terminal domain
GST_C_2 PF13410 280 - 352 6e-17 Glutathione S-transferase, C-terminal domain
GST_C_3 PF14497 285 - 362 2.9e-07 Glutathione S-transferase, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016564)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G44000
fragaria_vesca FvH4_2g20660
prunus_persica Prupe.8G216400_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0286841
rosa_laevigata RLG00000012532
rosa_multiflora Rmu_sc0011217.1_g000001 Rmu_sc0018293.1_g000001
rosa_roxburghii Rroxscaffold_7G00181880
rosa_rugosa Rorug06G0183900
rosa_samantha Rh6AG296000 Rh6BG300300 Rh6CG308200 Rh6DG292000
rosa_wichuraiana Rw6G025520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
AccBSI CCGCTC 2 cut(s) 476, 599
AccI GTMKAC 1 cut(s) 585
AccII CGCG 3 cut(s) 120, 249, 516
AccIII TCCGGA 1 cut(s) 526
AclWI GGATC 6 cut(s) 296, 518, 531, 744, 1170, 1183
AcsI RAATTY 1 cut(s) 500
AcuI CTGAAG 1 cut(s) 1178
AcvI CACGTG 1 cut(s) 267
AcyI GRCGYC 2 cut(s) 453, 1233
AdeI CACNNNGTG 1 cut(s) 267
AfaI GTAC 2 cut(s) 608, 660
AgsI TTSAA 7 cut(s) 58, 505, 697, 757, 770, 874, 1003
AhdI GACNNNNNGTC 2 cut(s) 616, 1219
AjnI CCWGG 6 cut(s) 254, 332, 404, 494, 1010, 1144
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 7 cut(s) 30, 69, 199, 289, 675, 889, 1021
AluI AGCT 7 cut(s) 30, 69, 199, 289, 675, 889, 1021
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 469, 840
AlwI GGATC 6 cut(s) 296, 518, 531, 744, 1170, 1183
Ama87I CYCGRG 2 cut(s) 546, 727
Aor13HI TCCGGA 1 cut(s) 526
AoxI GGCC 8 cut(s) 93, 208, 226, 391, 408, 442, 717, 1199
ApaI GGGCCC 3 cut(s) 97, 212, 412
ApeKI GCWGC 7 cut(s) 22, 251, 1149, 1247, 1250, 1253, 1256
ApoI RAATTY 1 cut(s) 500
AspLEI GCGC 1 cut(s) 251
AsuC2I CCSGG 7 cut(s) 225, 480, 521, 547, 548, 728, 729
AsuHPI GGTGA 5 cut(s) 115, 175, 184, 735, 952
AvaI CYCGRG 2 cut(s) 546, 727
AvaII GGWCC 3 cut(s) 166, 618, 777
BaeGI GKGCMC 4 cut(s) 97, 212, 412, 485
BaeI ACNNNNGTAYC 2 cut(s) 598, 631
BamHI GGATCC 2 cut(s) 523, 1175
BanII GRGCYC 5 cut(s) 32, 97, 212, 412, 436
BbrPI CACGTG 1 cut(s) 267
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 7 cut(s) 9, 263, 1161, 1259, 1262, 1265, 1268
BccI CCATC 2 cut(s) 116, 220
BceAI ACGGC 2 cut(s) 440, 505
BcgI CGANNNNNNTGC 2 cut(s) 406, 440
BciT130I CCWGG 6 cut(s) 256, 334, 406, 496, 1012, 1146
BciVI GTATCC 2 cut(s) 523, 1041
BclI TGATCA 1 cut(s) 1104
BcnI CCSGG 7 cut(s) 225, 480, 521, 547, 548, 728, 729
BcoDI GTCTC 2 cut(s) 469, 840
BfmI CTRYAG 1 cut(s) 814
BfuI GTATCC 2 cut(s) 523, 1041
BlpI GCTNAGC 1 cut(s) 26
BmcAI AGTACT 1 cut(s) 660
Bme18I GGWCC 3 cut(s) 166, 618, 777
BmeRI GACNNNNNGTC 2 cut(s) 616, 1219
BmeT110I CYCGRG 2 cut(s) 546, 727
BmiI GGNNCC 9 cut(s) 95, 96, 168, 209, 210, 410, 493, 525, 1177
BmsI GCATC 2 cut(s) 631, 1192
BpmI CTGGAG 1 cut(s) 316
Bpu1102I GCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 7 cut(s) 225, 480, 521, 547, 548, 728, 729
BsaAI YACGTR 2 cut(s) 267, 388
BsaHI GRCGYC 2 cut(s) 453, 1233
BsaJI CCNNGG 7 cut(s) 89, 404, 405, 495, 546, 727, 1144
BsaWI WCCGGW 2 cut(s) 526, 972
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 158, 188, 356, 386
Bse1I ACTGG 3 cut(s) 15, 720, 885
BseAI TCCGGA 1 cut(s) 526
BseBI CCWGG 6 cut(s) 256, 334, 406, 496, 1012, 1146
BseDI CCNNGG 7 cut(s) 89, 404, 405, 495, 546, 727, 1144
BseGI GGATG 5 cut(s) 111, 212, 646, 952, 969
BseMII CTCAG 2 cut(s) 17, 845
BseNI ACTGG 3 cut(s) 15, 720, 885
BseRI GAGGAG 6 cut(s) 22, 61, 64, 67, 70, 221
BseSI GKGCMC 4 cut(s) 97, 212, 412, 485
BseXI GCAGC 7 cut(s) 9, 263, 1161, 1259, 1262, 1265, 1268
BseYI CCCAGC 1 cut(s) 285
BsgI GTGCAG 1 cut(s) 586
Bsh1236I CGCG 3 cut(s) 120, 249, 516
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 8 cut(s) 95, 210, 228, 393, 410, 444, 719, 1201
BsiEI CGRYCG 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 2 cut(s) 546, 727
BsiSI CCGG 9 cut(s) 164, 225, 479, 521, 527, 547, 728, 973, 1173
BslFI GGGAC 6 cut(s) 121, 268, 443, 563, 1207, 1226
BsmAI GTCTC 2 cut(s) 469, 840
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 6 cut(s) 121, 268, 443, 563, 1207, 1226
BsmI GAATGC 1 cut(s) 1152
BsnI GGCC 8 cut(s) 95, 210, 228, 393, 410, 444, 719, 1201
BsoBI CYCGRG 2 cut(s) 546, 727
Bsp120I GGGCCC 3 cut(s) 93, 208, 408
Bsp1286I GDGCHC 6 cut(s) 32, 97, 212, 412, 436, 485
Bsp13I TCCGGA 1 cut(s) 526
Bsp143I GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
Bsp1720I GCTNAGC 1 cut(s) 26
BspANI GGCC 8 cut(s) 95, 210, 228, 393, 410, 444, 719, 1201
BspCNI CTCAG 2 cut(s) 18, 844
BspEI TCCGGA 1 cut(s) 526
BspFNI CGCG 3 cut(s) 120, 249, 516
BspHI TCATGA 1 cut(s) 276
BspLI GGNNCC 9 cut(s) 95, 96, 168, 209, 210, 410, 493, 525, 1177
BspPI GGATC 6 cut(s) 296, 518, 531, 744, 1170, 1183
BsrBI CCGCTC 2 cut(s) 476, 599
BsrI ACTGG 3 cut(s) 15, 720, 885
BssECI CCNNGG 7 cut(s) 89, 404, 405, 495, 546, 727, 1144
BssMI GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
BssNI GRCGYC 2 cut(s) 453, 1233
Bst2UI CCWGG 6 cut(s) 256, 334, 406, 496, 1012, 1146
Bst4CI ACNGT 3 cut(s) 244, 614, 625
Bst6I CTCTTC 1 cut(s) 962
BstACI GRCGYC 2 cut(s) 453, 1233
BstBAI YACGTR 2 cut(s) 267, 388
BstC8I GCNNGC 4 cut(s) 291, 750, 942, 1237
BstDEI CTNAG 2 cut(s) 26, 831
BstDSI CCRYGG 1 cut(s) 89
BstF5I GGATG 5 cut(s) 111, 212, 646, 952, 969
BstFNI CGCG 3 cut(s) 120, 249, 516
BstHHI GCGC 1 cut(s) 251
BstKTI GATC 8 cut(s) 304, 526, 739, 898, 982, 1026, 1107, 1178
BstMAI GTCTC 2 cut(s) 469, 840
BstMBI GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
BstMCI CGRYCG 1 cut(s) 616
BstMWI GCNNNNNNNGC 7 cut(s) 399, 489, 672, 912, 921, 1253, 1256
BstNI CCWGG 6 cut(s) 256, 334, 406, 496, 1012, 1146
BstNSI RCATGY 1 cut(s) 1054
BstSFI CTRYAG 1 cut(s) 814
BstSLI GKGCMC 4 cut(s) 97, 212, 412, 485
BstUI CGCG 3 cut(s) 120, 249, 516
BstV1I GCAGC 7 cut(s) 9, 263, 1161, 1259, 1262, 1265, 1268
BstX2I RGATCY 3 cut(s) 523, 736, 1175
BstXI CCANNNNNNTGG 1 cut(s) 636
BstYI RGATCY 3 cut(s) 523, 736, 1175
BsuI GTATCC 2 cut(s) 523, 1041
BsuRI GGCC 8 cut(s) 95, 210, 228, 393, 410, 444, 719, 1201
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 5 cut(s) 111, 212, 646, 952, 969
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 3 cut(s) 630, 864, 878
Cac8I GCNNGC 4 cut(s) 291, 750, 942, 1237
CciI TCATGA 1 cut(s) 276
CfoI GCGC 1 cut(s) 251
Cfr9I CCCGGG 2 cut(s) 546, 727
CseI GACGC 4 cut(s) 109, 461, 946, 1241
Csp6I GTAC 2 cut(s) 607, 659
CviAII CATG 4 cut(s) 277, 1043, 1051, 1108
CviQI GTAC 2 cut(s) 607, 659
DdeI CTNAG 2 cut(s) 26, 831
DpnI GATC 8 cut(s) 303, 525, 738, 897, 981, 1025, 1106, 1177
DpnII GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
DraIII CACNNNGTG 1 cut(s) 267
DrdI GACNNNNNNGTC 1 cut(s) 455
DriI GACNNNNNGTC 2 cut(s) 616, 1219
DseDI GACNNNNNNGTC 1 cut(s) 455
Eam1104I CTCTTC 1 cut(s) 962
Eam1105I GACNNNNNGTC 2 cut(s) 616, 1219
EarI CTCTTC 1 cut(s) 962
EciI GGCGGA 1 cut(s) 104
Ecl136II GAGCTC 1 cut(s) 30
Eco24I GRGCYC 5 cut(s) 32, 97, 212, 412, 436
Eco47I GGWCC 3 cut(s) 166, 618, 777
Eco53kI GAGCTC 1 cut(s) 30
Eco57I CTGAAG 1 cut(s) 1178
Eco72I CACGTG 1 cut(s) 267
Eco88I CYCGRG 2 cut(s) 546, 727
EcoICRI GAGCTC 1 cut(s) 30
EcoO109I RGGNCCY 3 cut(s) 94, 208, 442
EcoRI GAATTC 1 cut(s) 500
EcoRII CCWGG 6 cut(s) 254, 332, 404, 494, 1010, 1144
EcoT38I GRGCYC 5 cut(s) 32, 97, 212, 412, 436
Esp3I CGTCTC 1 cut(s) 469
FaeI CATG 4 cut(s) 280, 1046, 1054, 1111
FaqI GGGAC 6 cut(s) 121, 268, 443, 563, 1207, 1226
FatI CATG 4 cut(s) 276, 1042, 1050, 1107
FauI CCCGC 3 cut(s) 365, 521, 592
FauNDI CATATG 1 cut(s) 847
FbaI TGATCA 1 cut(s) 1104
FblI GTMKAC 1 cut(s) 585
FokI GGATG 5 cut(s) 98, 199, 653, 956, 959
FriOI GRGCYC 5 cut(s) 32, 97, 212, 412, 436
GlaI GCGC 1 cut(s) 250
GsaI CCCAGC 1 cut(s) 289
GsuI CTGGAG 1 cut(s) 316
HaeIII GGCC 8 cut(s) 95, 210, 228, 393, 410, 444, 719, 1201
HapII CCGG 9 cut(s) 164, 225, 479, 521, 527, 547, 728, 973, 1173
HgaI GACGC 4 cut(s) 109, 461, 946, 1241
HhaI GCGC 1 cut(s) 251
Hin1I GRCGYC 2 cut(s) 453, 1233
Hin1II CATG 4 cut(s) 280, 1046, 1054, 1111
Hin6I GCGC 1 cut(s) 249
HinP1I GCGC 1 cut(s) 249
HinfI GANTC 7 cut(s) 58, 571, 766, 810, 1069, 1214, 1241
HpaII CCGG 9 cut(s) 164, 225, 479, 521, 527, 547, 728, 973, 1173
HphI GGTGA 5 cut(s) 115, 175, 184, 735, 952
Hpy166II GTNNAC 2 cut(s) 586, 621
Hpy188I TCNGA 6 cut(s) 301, 431, 576, 708, 1180, 1197
Hpy188III TCNNGA 6 cut(s) 277, 505, 527, 594, 770, 1187
Hpy8I GTNNAC 2 cut(s) 586, 621
Hpy99I CGWCG 3 cut(s) 452, 455, 614
HpyAV CCTTC 4 cut(s) 46, 94, 1049, 1106
HpyCH4III ACNGT 3 cut(s) 244, 614, 625
HpyCH4IV ACGT 2 cut(s) 266, 387
HpyCH4V TGCA 8 cut(s) 9, 567, 644, 666, 752, 1008, 1082, 1092
HpyF10VI GCNNNNNNNGC 7 cut(s) 399, 489, 672, 912, 921, 1253, 1256
HpyF3I CTNAG 2 cut(s) 26, 831
HpySE526I ACGT 2 cut(s) 266, 387
Hsp92I GRCGYC 2 cut(s) 453, 1233
Hsp92II CATG 4 cut(s) 280, 1046, 1054, 1111
HspAI GCGC 1 cut(s) 249
Kpn2I TCCGGA 1 cut(s) 526
Ksp22I TGATCA 1 cut(s) 1104
Kzo9I GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
LmnI GCTCC 5 cut(s) 35, 74, 204, 481, 497
Lsp1109I GCAGC 7 cut(s) 9, 263, 1161, 1259, 1262, 1265, 1268
LweI GCATC 2 cut(s) 631, 1192
MaeII ACGT 2 cut(s) 266, 387
MaeIII GTNAC 4 cut(s) 121, 190, 722, 1055
MalI GATC 8 cut(s) 303, 525, 738, 897, 981, 1025, 1106, 1177
MbiI CCGCTC 2 cut(s) 476, 599
MboI GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
MboII GAAGA 2 cut(s) 905, 949
MflI RGATCY 3 cut(s) 523, 736, 1175
MhlI GDGCHC 6 cut(s) 32, 97, 212, 412, 436, 485
MluCI AATT 5 cut(s) 500, 689, 697, 1093, 1164
MlyI GAGTC 3 cut(s) 565, 819, 1208
MroI TCCGGA 1 cut(s) 526
MseI TTAA 2 cut(s) 1227, 1273
MslI CAYNNNNRTG 1 cut(s) 131
MspI CCGG 9 cut(s) 164, 225, 479, 521, 527, 547, 728, 973, 1173
Mva1269I GAATGC 1 cut(s) 1152
MvaI CCWGG 6 cut(s) 256, 334, 406, 496, 1012, 1146
MvnI CGCG 3 cut(s) 120, 249, 516
MwoI GCNNNNNNNGC 7 cut(s) 399, 489, 672, 912, 921, 1253, 1256
NciI CCSGG 7 cut(s) 225, 480, 521, 547, 548, 728, 729
NdeI CATATG 1 cut(s) 847
NdeII GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
NlaIII CATG 4 cut(s) 280, 1046, 1054, 1111
NlaIV GGNNCC 9 cut(s) 95, 96, 168, 209, 210, 410, 493, 525, 1177
NmuCI GTSAC 3 cut(s) 121, 190, 1055
NspI RCATGY 1 cut(s) 1054
PagI TCATGA 1 cut(s) 276
PasI CCCWGGG 1 cut(s) 405
PcsI WCGNNNNNNNCGW 5 cut(s) 297, 453, 459, 675, 684
PctI GAATGC 1 cut(s) 1152
PfeI GAWTC 4 cut(s) 58, 766, 1069, 1241
PflFI GACNNNGTC 1 cut(s) 455
PleI GAGTC 3 cut(s) 565, 818, 1208
PmaCI CACGTG 1 cut(s) 267
PmlI CACGTG 1 cut(s) 267
PpsI GAGTC 3 cut(s) 565, 818, 1208
Ppu21I YACGTR 2 cut(s) 267, 388
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 6 cut(s) 254, 332, 404, 494, 1010, 1144
PspCI CACGTG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 285
PspGI CCWGG 6 cut(s) 254, 332, 404, 494, 1010, 1144
PspN4I GGNNCC 9 cut(s) 95, 96, 168, 209, 210, 410, 493, 525, 1177
PspOMI GGGCCC 3 cut(s) 93, 208, 408
PsuI RGATCY 3 cut(s) 523, 736, 1175
PsyI GACNNNGTC 1 cut(s) 455
RsaI GTAC 2 cut(s) 608, 660
RsaNI GTAC 2 cut(s) 607, 659
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 32
SaqAI TTAA 2 cut(s) 1227, 1273
Sau3AI GATC 8 cut(s) 301, 523, 736, 895, 979, 1023, 1104, 1175
ScaI AGTACT 1 cut(s) 660
SchI GAGTC 3 cut(s) 565, 819, 1208
SduI GDGCHC 6 cut(s) 32, 97, 212, 412, 436, 485
SfaNI GCATC 2 cut(s) 631, 1192
SfcI CTRYAG 1 cut(s) 814
SinI GGWCC 3 cut(s) 166, 618, 777
SmaI CCCGGG 2 cut(s) 548, 729
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 5 cut(s) 500, 689, 697, 1093, 1164
SstI GAGCTC 1 cut(s) 32
TaaI ACNGT 3 cut(s) 244, 614, 625
TaiI ACGT 2 cut(s) 269, 390
TaqI TCGA 6 cut(s) 340, 447, 537, 687, 978, 1026
TaqII GACCGA 1 cut(s) 606
TasI AATT 5 cut(s) 500, 689, 697, 1093, 1164
TatI WGTACW 1 cut(s) 658
TauI GCSGC 4 cut(s) 120, 274, 476, 1204
TfiI GAWTC 4 cut(s) 58, 766, 1069, 1241
Tru1I TTAA 2 cut(s) 1227, 1273
Tru9I TTAA 2 cut(s) 1227, 1273
TscAI CASTG 3 cut(s) 630, 864, 885
TseFI GTSAC 3 cut(s) 121, 190, 1055
TseI GCWGC 7 cut(s) 22, 251, 1149, 1247, 1250, 1253, 1256
Tsp45I GTSAC 3 cut(s) 121, 190, 1055
TspDTI ATGAA 4 cut(s) 17, 726, 945, 1031
TspGWI ACGGA 2 cut(s) 178, 500
TspMI CCCGGG 2 cut(s) 546, 727
TspRI CASTG 3 cut(s) 630, 864, 885
Tth111I GACNNNGTC 1 cut(s) 455
VpaK11BI GGWCC 3 cut(s) 166, 618, 777
XapI RAATTY 1 cut(s) 500
XceI RCATGY 1 cut(s) 1054
XmaI CCCGGG 2 cut(s) 546, 727
XmiI GTMKAC 1 cut(s) 585
ZrmI AGTACT 1 cut(s) 660
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.