AT5G48460

Calponin homology domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013823
Physical Location & Seq
Reverse (-)
19636045 .. 19639529
3485 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G48460.1

Sequence Viewer

Length: 1965 bp
ATGTCAGGATTCGTCGGGATTTTGGTATCGGATCCATGGTTACAGAACCAATTCACGCAAGTGGAGCTTCGGAGTTTGAAATCTCACTTCACGAGCATGAAGAGAGAGAGCGGGAAGCTAACAGTATCAGACCTCGCGTCAAGGATGGGGAAATCAAAAGTAGTTGGAGATCAGAATCTAAGCAACGAAGAACGAGCGACTTTGATCCAAAATTTTCATCCGAATTTGAATGATGAAGTCGATTTCGAGTTTTACTTAAGGATTTATCTGAATCTTCAAGCGCATGTAAACGCAATAATCGGAAGCGGTGTTAAAAACTCATCTGCGTTTCTTAAAGCAGCTACAACAACTTTGTTGCATACTATTAGTGATTCTGAGAAATCTTCTTATGTTGCTCATATCAATAATTATCTCTCTGGAGATGAGTTTCTAAACAAGTGCCTTCCGATTAATCCGTCTTCGAATGATCTATTCGAAGTTGCAAAAGATGGTGTTTTGCTTTGTAAGCTTATAAATGTGGCGGTTCCTGGAACAATCGATGAACGAGCGATTAATACTAAGAGTATGCTTAATCCATGGGAGAGAAATGAAAATCATACGCTTTGTCTTAACTCTGCCAAGGCTATTGGGTGTACTGTGGTTAATATAGGAACACAAGATATCATTGAAGGAAGGCGTCATCTTGTGCTCGGTGTGATCTCTCAAATCATTAAGATACAATTGCTAGCAGACCTAAACTTGAAGAAAACTCCCCAGCTGGTGGAGTTGGTCGGTGATAGCAAGGATGTGGAAGAGCTAATGAGTTTACCACCTGAGAAGATCTTATTGAGATGGATGAATTTTCAGTTGAGAAAAACTGAATACAAGAAAACCGTCACAAACTTCTCTTCTGATGTTAAGGATGCGGAAGCTTACACTAATCTATTAAATGTCCTGGCACCAGAGCACAAGAATCCATCACATTTAGCAGTTAAAAGCTCATTTGAGAGAGCAAAACTTGTTCTCGAACATGCAGACAAAATGGGATGTAGGAGATACTTGACGGCGAAAGATATTGTTGAGGGTTCTCCAAATCTTAATCTTGCTTTTGTAGCACATATATTCCAGCACAGGAACGGACTTTCAACACAAACGAAACAGATATCTTTTCTTGAAAATCTAGCTGATGACATTCAAATTTCAAGAGAAGAAAAAGCATTCCGGTTTTGGATTAACAGCTTTGATGGTTCAGTATACATAAACAACGTGTTTGAAGATCTCAGAGATGGGTGGATACTATTGCAGACACTAGACAAAGTGTCTCCAGGCATTGTTAATTGGAAAGTATCAAGCAAGCCTCCAATCAAATTGCCATTCAAGAAAGTAGAAAACTGTAATCAAGTTGTGAAATTAGGGAAGCAGCTCAAGTTCTCTTTGGTTAACATTGCTGGAAATGACATTGTTCAAGGAAACAAGAAACTCATACTAGCATACTTATGGCAATTGATGAGATACAACATCCTGCAACTTCTTAAGAACTTGAGACTACATTCAAACGGCAAAGAGATAACAGACGCTGATATATTGGAATGGGCAAATGCTAAAGTGAGAAACAACGGATGCAAAACACGCATGTACAGTTTCAGGGACAAGAGCTTATCCGATGGGGTATTTTTCTTGGAGCTGCTGAGTTCAGTGCAGCCAAGATCCGTAAACTGGAGTCTTGTTACAAACGGAGTTACAGATGAAGAGAAAAAGATGAATGCAACTTATGTGATTAGCATAGCCAGGAAACTTGGTTGCTCCATTTTCTTACTCCCTGAAGATATAATCGAGGTGAATCAGAAGATGATGCTGACATTGACGGCGAGTATAATGTATTGGACTCTAAAGCAACCTCTTCATCTGAATAAGCCTATCGGATCTCCTGATAGCCACAATGGAAGTTTACTCGATGATTCTACGTCAGATTCCTCTATAGAATAA

Protein Analysis

654

Amino Acids

73.66

Weight (kDa)

8.52

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 143 - 238 5.7e-17 Calponin homology (CH) domain
CH PF00307 270 - 371 4.6e-22 Calponin homology (CH) domain
CH PF00307 396 - 499 6.8e-20 Calponin homology (CH) domain
CH PF00307 518 - 620 2.1e-19 Calponin homology (CH) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013823)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G48460
fragaria_vesca FvH4_2g17780 FvH4_2g17780
malus_domestica MD05G1159000.v1.1 MD10G1151800.v1.1
prunus_persica Prupe.8G190200_v2.0.a1
pyrus_communis pycom05g15090 pycom10g13080
rosa_chinensis RchiOBHm_Chr6g0282801
rosa_laevigata RLG00000012868
rosa_multiflora Rmu_sc0018221.1_g000009
rosa_roxburghii Rroxscaffold_7G00186020
rosa_rugosa Rorug06G0149800
rosa_samantha Rh6AG262800 Rh6CG265000 Rh6DG257500
rosa_wichuraiana Rw6G022700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 512
AccB1I GGYRCC 1 cut(s) 937
AccB7I CCANNNNNTGG 1 cut(s) 760
AccBSI CCGCTC 1 cut(s) 111
AccI GTMKAC 1 cut(s) 1233
AccII CGCG 1 cut(s) 137
AciI CCGC 4 cut(s) 111, 306, 521, 905
AclWI GGATC 5 cut(s) 26, 39, 199, 1680, 1909
AcsI RAATTY 4 cut(s) 211, 223, 838, 1176
AcuI CTGAAG 1 cut(s) 1821
AcyI GRCGYC 1 cut(s) 676
AfaI GTAC 2 cut(s) 634, 1616
AfiI CCNNNNNNNGG 2 cut(s) 760, 1695
AflII CTTAAG 2 cut(s) 256, 1511
AflIII ACRYGT 1 cut(s) 1245
AhdI GACNNNNNGTC 2 cut(s) 136, 1297
AjnI CCWGG 4 cut(s) 526, 933, 1303, 1766
AleI CACNNNNGTG 1 cut(s) 59
Alw21I GWGCWC 2 cut(s) 690, 948
Alw26I GTCTC 2 cut(s) 1305, 1516
AlwI GGATC 5 cut(s) 26, 39, 199, 1680, 1909
AlwNI CAGNNNCTG 1 cut(s) 1556
ApeKI GCWGC 4 cut(s) 338, 1399, 1663, 1678
ApoI RAATTY 4 cut(s) 211, 223, 838, 1176
AseI ATTAAT 2 cut(s) 450, 552
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 283
AsuHPI GGTGA 2 cut(s) 785, 1828
AsuII TTCGAA 2 cut(s) 461, 474
AsuNHI GCTAGC 1 cut(s) 724
BamHI GGATCC 1 cut(s) 31
BanI GGYRCC 1 cut(s) 937
BauI CACGAG 1 cut(s) 91
BbsI GAAGAC 1 cut(s) 450
Bbv12I GWGCWC 2 cut(s) 690, 948
BbvI GCAGC 4 cut(s) 350, 1411, 1650, 1690
BccI CCATC 7 cut(s) 139, 482, 825, 964, 1217, 1259, 1637
BceAI ACGGC 3 cut(s) 1059, 1552, 1860
BciT130I CCWGG 4 cut(s) 528, 935, 1305, 1768
BciVI GTATCC 1 cut(s) 1266
BcoDI GTCTC 2 cut(s) 1305, 1516
BfaI CTAG 4 cut(s) 725, 1160, 1289, 1466
BfmI CTRYAG 1 cut(s) 1956
BfrI CTTAAG 2 cut(s) 256, 1511
BfuI GTATCC 1 cut(s) 1266
BglII AGATCT 2 cut(s) 819, 1255
BisI GCNGC 4 cut(s) 339, 1400, 1664, 1679
BlsI GCNGC 4 cut(s) 340, 1401, 1665, 1680
Bme1390I CCNGG 4 cut(s) 528, 935, 1305, 1768
BmeRI GACNNNNNGTC 2 cut(s) 136, 1297
BmiI GGNNCC 3 cut(s) 33, 525, 939
BmrFI CCNGG 4 cut(s) 528, 935, 1305, 1768
BmsI GCATC 3 cut(s) 892, 1589, 1821
BmtI GCTAGC 1 cut(s) 728
BpiI GAAGAC 1 cut(s) 450
BpmI CTGGAG 3 cut(s) 438, 1287, 1717
Bpu14I TTCGAA 2 cut(s) 461, 474
BpuEI CTTGAG 2 cut(s) 1388, 1540
Bsa29I ATCGAT 1 cut(s) 537
BsaBI GATNNNNATC 1 cut(s) 174
BsaHI GRCGYC 1 cut(s) 676
BsaJI CCNNGG 3 cut(s) 35, 575, 618
BsaWI WCCGGW 1 cut(s) 1200
Bsc4I CCNNNNNNNGG 2 cut(s) 760, 1695
Bse1I ACTGG 1 cut(s) 1700
Bse3DI GCAATG 1 cut(s) 1422
Bse8I GATNNNNATC 1 cut(s) 174
BseBI CCWGG 4 cut(s) 528, 935, 1305, 1768
BseCI ATCGAT 1 cut(s) 537
BseDI CCNNGG 3 cut(s) 35, 575, 618
BseGI GGATG 8 cut(s) 150, 217, 790, 840, 907, 1031, 1497, 1604
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 2 cut(s) 760, 1695
BseMI GCAATG 1 cut(s) 1422
BseMII CTCAG 4 cut(s) 366, 804, 1273, 1658
BseNI ACTGG 1 cut(s) 1700
BseXI GCAGC 4 cut(s) 350, 1411, 1650, 1690
BseYI CCCAGC 1 cut(s) 753
BsgI GTGCAG 1 cut(s) 1697
Bsh1236I CGCG 1 cut(s) 137
BshNI GGYRCC 1 cut(s) 937
BshVI ATCGAT 1 cut(s) 537
BsiHKAI GWGCWC 2 cut(s) 690, 948
BsiSI CCGG 1 cut(s) 1201
BslFI GGGAC 1 cut(s) 1640
BslI CCNNNNNNNGG 2 cut(s) 760, 1695
BsmAI GTCTC 2 cut(s) 1305, 1516
BsmFI GGGAC 1 cut(s) 1640
BsmI GAATGC 2 cut(s) 1196, 1747
Bsp119I TTCGAA 2 cut(s) 461, 474
Bsp1286I GDGCHC 2 cut(s) 690, 948
Bsp1407I TGTACA 1 cut(s) 1614
Bsp143I GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
Bsp19I CCATGG 2 cut(s) 35, 575
BspACI CCGC 4 cut(s) 111, 306, 521, 905
BspCNI CTCAG 4 cut(s) 367, 805, 1272, 1659
BspDI ATCGAT 1 cut(s) 537
BspFNI CGCG 1 cut(s) 137
BspLI GGNNCC 3 cut(s) 33, 525, 939
BspOI GCTAGC 1 cut(s) 728
BspPI GGATC 5 cut(s) 26, 39, 199, 1680, 1909
BspQI GCTCTTC 1 cut(s) 786
BspT104I TTCGAA 2 cut(s) 461, 474
BspT107I GGYRCC 1 cut(s) 937
BspTI CTTAAG 2 cut(s) 256, 1511
BsrBI CCGCTC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 1422
BsrGI TGTACA 1 cut(s) 1614
BsrI ACTGG 1 cut(s) 1700
BssECI CCNNGG 3 cut(s) 35, 575, 618
BssMI GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
BssNAI GTATAC 1 cut(s) 1234
BssNI GRCGYC 1 cut(s) 676
BssSI CACGAG 1 cut(s) 91
BssT1I CCWWGG 3 cut(s) 35, 575, 618
Bst1107I GTATAC 1 cut(s) 1234
Bst2BI CACGAG 1 cut(s) 91
Bst2UI CCWGG 4 cut(s) 528, 935, 1305, 1768
Bst4CI ACNGT 5 cut(s) 124, 637, 874, 1373, 1619
Bst6I CTCTTC 5 cut(s) 95, 786, 892, 1722, 1884
BstACI GRCGYC 1 cut(s) 676
BstAFI CTTAAG 2 cut(s) 256, 1511
BstAUI TGTACA 1 cut(s) 1614
BstBI TTCGAA 2 cut(s) 461, 474
BstC8I GCNNGC 2 cut(s) 726, 1334
BstDEI CTNAG 6 cut(s) 179, 375, 558, 813, 1259, 1667
BstDSI CCRYGG 2 cut(s) 35, 575
BstF5I GGATG 8 cut(s) 150, 217, 790, 840, 907, 1031, 1497, 1604
BstFNI CGCG 1 cut(s) 137
BstHHI GCGC 1 cut(s) 283
BstKTI GATC 9 cut(s) 34, 172, 207, 469, 699, 822, 1258, 1688, 1904
BstMAI GTCTC 2 cut(s) 1305, 1516
BstMBI GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
BstMWI GCNNNNNNNGC 4 cut(s) 64, 505, 1091, 1608
BstNI CCWGG 4 cut(s) 528, 935, 1305, 1768
BstNSI RCATGY 3 cut(s) 287, 1013, 1615
BstSCI CCNGG 4 cut(s) 526, 933, 1303, 1766
BstSFI CTRYAG 1 cut(s) 1956
BstUI CGCG 1 cut(s) 137
BstV1I GCAGC 4 cut(s) 350, 1411, 1650, 1690
BstV2I GAAGAC 1 cut(s) 450
BstX2I RGATCY 5 cut(s) 31, 819, 1255, 1685, 1901
BstYI RGATCY 5 cut(s) 31, 819, 1255, 1685, 1901
BstZ17I GTATAC 1 cut(s) 1234
Bsu15I ATCGAT 1 cut(s) 537
BsuI GTATCC 1 cut(s) 1266
BsuTUI ATCGAT 1 cut(s) 537
BtgI CCRYGG 2 cut(s) 35, 575
BtsCI GGATG 8 cut(s) 150, 217, 790, 840, 907, 1031, 1497, 1604
BtsIMutI CAGTG 1 cut(s) 1680
Cac8I GCNNGC 2 cut(s) 726, 1334
CaiI CAGNNNCTG 1 cut(s) 1556
CfoI GCGC 1 cut(s) 283
ClaI ATCGAT 1 cut(s) 537
CseI GACGC 3 cut(s) 126, 665, 1562
Csp6I GTAC 2 cut(s) 633, 1615
CviAII CATG 6 cut(s) 36, 97, 284, 576, 1010, 1612
CviQI GTAC 2 cut(s) 633, 1615
DdeI CTNAG 6 cut(s) 179, 375, 558, 813, 1259, 1667
DpnI GATC 9 cut(s) 33, 171, 206, 468, 698, 821, 1257, 1687, 1903
DpnII GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
DriI GACNNNNNGTC 2 cut(s) 136, 1297
Eam1104I CTCTTC 5 cut(s) 95, 786, 892, 1722, 1884
Eam1105I GACNNNNNGTC 2 cut(s) 136, 1297
EarI CTCTTC 5 cut(s) 95, 786, 892, 1722, 1884
Eco130I CCWWGG 3 cut(s) 35, 575, 618
Eco32I GATATC 2 cut(s) 661, 1143
Eco57I CTGAAG 1 cut(s) 1821
EcoRII CCWGG 4 cut(s) 526, 933, 1303, 1766
EcoRV GATATC 2 cut(s) 661, 1143
EcoT14I CCWWGG 3 cut(s) 35, 575, 618
ErhI CCWWGG 3 cut(s) 35, 575, 618
FaeI CATG 6 cut(s) 39, 100, 287, 579, 1013, 1615
FalI AAGNNNNNCTT 2 cut(s) 51, 83
FaqI GGGAC 1 cut(s) 1640
FatI CATG 6 cut(s) 35, 96, 283, 575, 1009, 1611
FauI CCCGC 1 cut(s) 104
FblI GTMKAC 1 cut(s) 1233
Fnu4HI GCNGC 4 cut(s) 339, 1400, 1664, 1679
FokI GGATG 8 cut(s) 157, 204, 797, 847, 914, 1038, 1484, 1611
Fsp4HI GCNGC 4 cut(s) 339, 1400, 1664, 1679
FspBI CTAG 4 cut(s) 725, 1160, 1289, 1466
GlaI GCGC 1 cut(s) 282
GluI GCNGC 4 cut(s) 339, 1400, 1664, 1679
GsaI CCCAGC 1 cut(s) 757
GsuI CTGGAG 3 cut(s) 438, 1287, 1717
HapII CCGG 1 cut(s) 1201
HgaI GACGC 3 cut(s) 126, 665, 1562
HhaI GCGC 1 cut(s) 283
Hin1I GRCGYC 1 cut(s) 676
Hin1II CATG 6 cut(s) 39, 100, 287, 579, 1013, 1615
Hin6I GCGC 1 cut(s) 281
HinP1I GCGC 1 cut(s) 281
HincII GTYRAC 1 cut(s) 1420
HindII GTYRAC 1 cut(s) 1420
HindIII AAGCTT 2 cut(s) 506, 909
HpaI GTTAAC 1 cut(s) 1420
HpaII CCGG 1 cut(s) 1201
HphI GGTGA 2 cut(s) 785, 1828
Hpy166II GTNNAC 7 cut(s) 289, 633, 806, 1234, 1420, 1693, 1928
Hpy188III TCNNGA 9 cut(s) 6, 16, 91, 417, 1004, 1151, 1182, 1357, 1907
Hpy8I GTNNAC 7 cut(s) 289, 633, 806, 1234, 1420, 1693, 1928
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 3 cut(s) 452, 662, 666
HpyCH4III ACNGT 5 cut(s) 124, 637, 874, 1373, 1619
HpyCH4IV ACGT 2 cut(s) 1245, 1943
HpyCH4V TGCA 8 cut(s) 358, 482, 1013, 1282, 1504, 1602, 1678, 1745
HpyF10VI GCNNNNNNNGC 4 cut(s) 64, 505, 1091, 1608
HpyF3I CTNAG 6 cut(s) 179, 375, 558, 813, 1259, 1667
HpySE526I ACGT 2 cut(s) 1245, 1943
Hsp92I GRCGYC 1 cut(s) 676
Hsp92II CATG 6 cut(s) 39, 100, 287, 579, 1013, 1615
HspAI GCGC 1 cut(s) 281
KspAI GTTAAC 1 cut(s) 1420
Kzo9I GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
LguI GCTCTTC 1 cut(s) 786
LmnI GCTCC 3 cut(s) 64, 1660, 1787
Lsp1109I GCAGC 4 cut(s) 350, 1411, 1650, 1690
LweI GCATC 3 cut(s) 892, 1589, 1821
MaeI CTAG 4 cut(s) 725, 1160, 1289, 1466
MaeII ACGT 2 cut(s) 1245, 1943
MaeIII GTNAC 4 cut(s) 39, 874, 1705, 1717
MalI GATC 9 cut(s) 33, 171, 206, 468, 698, 821, 1257, 1687, 1903
MbiI CCGCTC 1 cut(s) 111
MboI GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
MfeI CAATTG 2 cut(s) 719, 1481
MflI RGATCY 5 cut(s) 31, 819, 1255, 1685, 1901
MhlI GDGCHC 2 cut(s) 690, 948
MlyI GAGTC 2 cut(s) 1708, 1858
MmeI TCCRAC 1 cut(s) 145
MnlI CCTC 6 cut(s) 143, 1054, 1347, 1807, 1887, 1963
MroXI GAANNNNTTC 1 cut(s) 50
MslI CAYNNNNRTG 3 cut(s) 59, 95, 1474
MspA1I CMGCKG 1 cut(s) 757
MspCI CTTAAG 2 cut(s) 256, 1511
MspI CCGG 1 cut(s) 1201
MspR9I CCNGG 4 cut(s) 528, 935, 1305, 1768
MunI CAATTG 2 cut(s) 719, 1481
Mva1269I GAATGC 2 cut(s) 1196, 1747
MvaI CCWGG 4 cut(s) 528, 935, 1305, 1768
MvnI CGCG 1 cut(s) 137
MwoI GCNNNNNNNGC 4 cut(s) 64, 505, 1091, 1608
NcoI CCATGG 2 cut(s) 35, 575
NdeII GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
NheI GCTAGC 1 cut(s) 724
NlaIII CATG 6 cut(s) 39, 100, 287, 579, 1013, 1615
NlaIV GGNNCC 3 cut(s) 33, 525, 939
NmuCI GTSAC 1 cut(s) 874
NspI RCATGY 3 cut(s) 287, 1013, 1615
NspV TTCGAA 2 cut(s) 461, 474
OliI CACNNNNGTG 1 cut(s) 59
PciSI GCTCTTC 1 cut(s) 786
PctI GAATGC 2 cut(s) 1196, 1747
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 8 cut(s) 9, 175, 271, 371, 952, 1819, 1937, 1949
PflMI CCANNNNNTGG 1 cut(s) 760
PfoI TCCNGGA 1 cut(s) 526
PkrI GCNGC 4 cut(s) 340, 1401, 1665, 1680
PleI GAGTC 2 cut(s) 1707, 1858
PpsI GAGTC 2 cut(s) 1707, 1858
PshBI ATTAAT 2 cut(s) 450, 552
PsiI TTATAA 1 cut(s) 512
Psp6I CCWGG 4 cut(s) 526, 933, 1303, 1766
PspFI CCCAGC 1 cut(s) 753
PspGI CCWGG 4 cut(s) 526, 933, 1303, 1766
PspN4I GGNNCC 3 cut(s) 33, 525, 939
PstNI CAGNNNCTG 1 cut(s) 1556
PsuI RGATCY 5 cut(s) 31, 819, 1255, 1685, 1901
PvuII CAGCTG 1 cut(s) 757
RsaI GTAC 2 cut(s) 634, 1616
RsaNI GTAC 2 cut(s) 633, 1615
RseI CAYNNNNRTG 3 cut(s) 59, 95, 1474
SapI GCTCTTC 1 cut(s) 786
SatI GCNGC 4 cut(s) 339, 1400, 1664, 1679
Sau3AI GATC 9 cut(s) 31, 169, 204, 466, 696, 819, 1255, 1685, 1901
SchI GAGTC 2 cut(s) 1708, 1858
ScrFI CCNGG 4 cut(s) 528, 935, 1305, 1768
SduI GDGCHC 2 cut(s) 690, 948
SfaNI GCATC 3 cut(s) 892, 1589, 1821
SfcI CTRYAG 1 cut(s) 1956
SfuI TTCGAA 2 cut(s) 461, 474
SmiMI CAYNNNNRTG 3 cut(s) 59, 95, 1474
SmlI CTYRAG 4 cut(s) 256, 1403, 1511, 1519
SmoI CTYRAG 4 cut(s) 256, 1403, 1511, 1519
SsiI CCGC 4 cut(s) 111, 306, 521, 905
SspMI CTAG 4 cut(s) 725, 1160, 1289, 1466
StyD4I CCNGG 4 cut(s) 526, 933, 1303, 1766
StyI CCWWGG 3 cut(s) 35, 575, 618
TaaI ACNGT 5 cut(s) 124, 637, 874, 1373, 1619
TaiI ACGT 2 cut(s) 1248, 1946
TaqI TCGA 8 cut(s) 240, 246, 461, 474, 537, 1005, 1812, 1932
TatI WGTACW 2 cut(s) 632, 1614
TfiI GAWTC 8 cut(s) 9, 175, 271, 371, 952, 1819, 1937, 1949
TscAI CASTG 1 cut(s) 1680
TseFI GTSAC 1 cut(s) 874
TseI GCWGC 4 cut(s) 338, 1399, 1663, 1678
Tsp45I GTSAC 1 cut(s) 874
TspDTI ATGAA 9 cut(s) 113, 206, 249, 555, 603, 851, 1740, 1754, 1871
TspGWI ACGGA 5 cut(s) 444, 1131, 1611, 1678, 1728
TspRI CASTG 1 cut(s) 1680
Van91I CCANNNNNTGG 1 cut(s) 760
Vha464I CTTAAG 2 cut(s) 256, 1511
VspI ATTAAT 2 cut(s) 450, 552
XapI RAATTY 4 cut(s) 211, 223, 838, 1176
XceI RCATGY 3 cut(s) 287, 1013, 1615
XmiI GTMKAC 1 cut(s) 1233
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 4 cut(s) 725, 1160, 1289, 1466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.