Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0013823

Physical Location & Seq

Genomic Coordinates Reverse (-)

28777615 .. 28782096

Gene Structure 4482 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1159000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGTCGGGGTACGTCGGCATTCTAGTCTCAGATCCATGGCTGCAGAATCAGTTCACCCAAGTGGAGCTCCGGAGCTTGAAATCCCATTTTATGAGCATGAAGAGAGAGAGTGGAAAGCTCACAGTTGGAGACTTGCCTGCCAAGATGTCAAGGCTCAAGGTCGTGGGATCAGATAATTTTACTGACCAAGACAGGGCTTCTTTCATTCAAGATTTGCATCCCAACCTCGATGAAGATGTCGATTTCGAATTCTTCCTCAGGGTTTATTTGCAACTCCAAGCTCATGCCACTGCAACCACAGGAAGTGGTGGAAAGAACAACTCTTCATCTGCCTTTCTCAAGGCTGCTACCACCACTTTGCTTCACACGATCAGCGAATCCGAGAAGGCATCCTATGTAGCTCATATCAATAACTACCTTGGACAGGATGATTTCCTTAAGACATATCTCCCTATTGATCCATCAACCAATGATTTATTCGAGATCGTAAAAGATGGAGTACTTTTATGTAAGCTGATCAATGTGGCGGTGCCTGGAACAATCGATGAACGAGCTATCAATACTAAACGAGTACTTAATCCATGGGAGAGAAATGAAAATCACACCTTGTGCCTCAACTCTGCTAAGGCTGTTGGATGTACTGTGGTCAATATTGGGACTCAGGATTTCATTGAAGGAAGGCGTCATCTTGTTCTTGGAGTGATTTCTCAGATAATCAAGATACAACTCTTGGCAGACCTCAACTTGAAGAAAACACCTCAATTGGTGGAGTTGGTTGACGATAGCAACGATGTGGAAGAGTTGATGAGTCTACCACCGGAAAAGATATTACTGAGATGGATGAATTTCCAATTGAAGAAAGCTGGATATCAGAAGATAGTCACAAACTTCTCCTCTGATGTGAAGGATGCAGAGGCTTATGCTCACCTCCTAAATGTTCTAGCACCGGAGCACAGTAAACCATCTGCATTGGATGCAAAGAATCCTATGGAAAGAGCAAAGTTAGTTCTTGAACATGCAGATAGGATGGGCTGCAAGAGATATTTGACCGCAAAAGATATTGTTGAAGGATCCCCGAATCTTAACCTTGCCTTTGTTGCACATATTTTCCAGCATAGAAATGGACTCTCAACACAAACAAAGCAGACATCATTCCTTGACAATTTGGAAGATGACACCCAAATCTCCAGAGAAGAGAGAGCATTTCGCTTTTGGATGAATAGTCTCGGGAATTCTTCATATATCAATAACGTCTTTGAAGACCTCAGAAACGGGTGGGCGCTTCTGGAGTCACTGGACAAGATTTCACCCGGCATTGTAAATTGGAAGATTGCAAACAAGCCTCCAATTAAAATGCCATTCAAGAAAGTAGAAAACTGCAACCAAGTTGTAAAAATTGGAAAGCAACTGAAGTTTTCTTTGGTAAATATTGCTGGAAATGACATCGTTCAGGGGAATAAAAAGTTAATACTTGCTTATTTGTGGCAATTGATGCGGTACAACATCCTCCAACTACTAAAGAACTTGAGATTTCATTCCCATGGGAAGGAAATTGCTGATGCTGATATTCTAGAATGGGCCAACACCAAAGTCAGCAGCACGGGAAGCCAGAGCTGCATGAAAAGTTTTAAGGATAAGAGTTTGTCGGATGGAATTTTCTTCCTTGAGTTGCTTAGCTGTGTGCAGCCTAGAGTGGTGAACTGGAGTGTTGTAACAAAAGGAGTAACCGATGAGGAGAAGAAGATGAATGCCACTTACATTATCAGTATTGCAAGAAAGCTCGGATGTTCGATATTTTTGCTTCCTGAAGACATAACTGAGGTGAATCAAAAGATGATTCTTACTTTGACTGCAAGCATTATGTACTGGTTTTTGAAACAGCCTGTCGAAGATCGACGGTCTGGGATTTCGGATAGTGAGGGTTCAAGCATTGTCTCCAATTCCACTACTGATGACTCCGCTTCGGAGTCATCAACGGAGGAGAATGGAAATATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005856 GO:0005884 GO:0006996 GO:0007010 GO:0007015 GO:0008092 GO:0008150 GO:0009987 GO:0015629 GO:0016043 GO:0022607 GO:0030029 GO:0030036 GO:0032432 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044085 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0044877 GO:0051015 GO:0051017 GO:0051639 GO:0051764 GO:0061572 GO:0071840 GO:0097435 GO:0099080 GO:0099081 GO:0099512 GO:0099513

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Protein Analysis

666

Amino Acids

74.63

Weight (kDa)

6.43

Isoelectric Point (pI)

40.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	152 - 240	4.3e-16	Calponin homology (CH) domain
CH	PF00307	272 - 373	1.4e-23	Calponin homology (CH) domain
CH	PF00307	398 - 501	3.1e-19	Calponin homology (CH) domain
CH	PF00307	520 - 624	3.9e-18	Calponin homology (CH) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013823)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G48460
fragaria_vesca	FvH4_2g17780 FvH4_2g17780
malus_domestica	MD05G1159000.v1.1 MD10G1151800.v1.1
prunus_persica	Prupe.8G190200_v2.0.a1
pyrus_communis	pycom05g15090 pycom10g13080
rosa_chinensis	RchiOBHm_Chr6g0282801
rosa_laevigata	RLG00000012868
rosa_multiflora	Rmu_sc0018221.1_g000009
rosa_roxburghii	Rroxscaffold_7G00186020
rosa_rugosa	Rorug06G0149800
rosa_samantha	Rh6AG262800 Rh6CG265000 Rh6DG257500
rosa_wichuraiana	Rw6G022700

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	529
AccI	GTMKAC	1 cut(s)	811
AccIII	TCCGGA	1 cut(s)	69
AciI	CCGC	4 cut(s)	527, 1050, 1495, 1959
AclWI	GGATC	5 cut(s)	26, 175, 452, 1065, 1078
AcsI	RAATTY	4 cut(s)	248, 844, 1231, 1653
AcuI	CTGAAG	2 cut(s)	1430, 1827
AcyI	GRCGYC	1 cut(s)	682
AdeI	CACNNNGTG	1 cut(s)	609
AfaI	GTAC	6 cut(s)	11, 501, 573, 640, 1499, 1865
AfiI	CCNNNNNNNGG	3 cut(s)	193, 424, 1546
AflII	CTTAAG	1 cut(s)	437
AjnI	CCWGG	1 cut(s)	532
AjuI	GAANNNNNNNTTGG	4 cut(s)	461, 493, 1403, 1435
AleI	CACNNNNGTG	1 cut(s)	59
Alw21I	GWGCWC	2 cut(s)	69, 954
Alw26I	GTCTC	4 cut(s)	31, 123, 1229, 1939
AlwI	GGATC	5 cut(s)	26, 175, 452, 1065, 1078
Ama87I	CYCGRG	1 cut(s)	1226
Aor13HI	TCCGGA	1 cut(s)	69
AoxI	GGCC	1 cut(s)	1578
ApeKI	GCWGC	6 cut(s)	40, 344, 1032, 1596, 1614, 1684
ApoI	RAATTY	4 cut(s)	248, 844, 1231, 1653
Asp700I	GAANNNNTTC	1 cut(s)	50
AspLEI	GCGC	1 cut(s)	1282
AspS9I	GGNCC	1 cut(s)	1578
AsuC2I	CCSGG	1 cut(s)	1311
AsuHPI	GGTGA	5 cut(s)	46, 917, 1299, 1708, 1834
AsuII	TTCGAA	1 cut(s)	246
AvaI	CYCGRG	1 cut(s)	1226
AxyI	CCTNAGG	1 cut(s)	257
BamHI	GGATCC	1 cut(s)	1070
BanI	GGYRCC	1 cut(s)	529
BanII	GRGCYC	1 cut(s)	69
BbsI	GAAGAC	2 cut(s)	1266, 1815
Bbv12I	GWGCWC	2 cut(s)	69, 954
BbvI	GCAGC	6 cut(s)	27, 331, 1019, 1601, 1608, 1696
BccI	CCATC	6 cut(s)	469, 488, 831, 970, 1021, 1643
BcgI	CGANNNNNNTGC	2 cut(s)	1780, 1814
BciT130I	CCWGG	1 cut(s)	534
BclI	TGATCA	1 cut(s)	516
BcnI	CCSGG	1 cut(s)	1311
BcoDI	GTCTC	4 cut(s)	31, 123, 1229, 1939
BfaI	CTAG	4 cut(s)	23, 941, 1571, 1689
BfmI	CTRYAG	1 cut(s)	41
BfoI	RGCGCY	1 cut(s)	1283
BfrI	CTTAAG	1 cut(s)	437
BisI	GCNGC	6 cut(s)	41, 345, 1033, 1597, 1615, 1685
BlpI	GCTNAGC	1 cut(s)	1673
BlsI	GCNGC	6 cut(s)	42, 346, 1034, 1598, 1616, 1686
BmcAI	AGTACT	2 cut(s)	501, 573
Bme1390I	CCNGG	2 cut(s)	534, 1311
BmeT110I	CYCGRG	1 cut(s)	1226
BmgT120I	GGNCC	1 cut(s)	1578
BmiI	GGNNCC	2 cut(s)	531, 1072
BmrFI	CCNGG	2 cut(s)	534, 1311
BmsI	GCATC	6 cut(s)	226, 398, 898, 964, 1482, 1549
BpiI	GAAGAC	2 cut(s)	1266, 1815
BpmI	CTGGAG	3 cut(s)	1171, 1307, 1723
Bpu10I	CCTNAGC	1 cut(s)	624
Bpu1102I	GCTNAGC	1 cut(s)	1673
Bpu14I	TTCGAA	1 cut(s)	246
BpuEI	CTTGAG	4 cut(s)	140, 323, 1546, 1685
BpuMI	CCSGG	1 cut(s)	1311
Bsa29I	ATCGAT	1 cut(s)	543
BsaBI	GATNNNNATC	1 cut(s)	216
BsaHI	GRCGYC	1 cut(s)	682
BsaJI	CCNNGG	4 cut(s)	35, 418, 581, 1540
BsaWI	WCCGGW	3 cut(s)	69, 817, 946
Bsc4I	CCNNNNNNNGG	3 cut(s)	193, 424, 1546
Bse1I	ACTGG	3 cut(s)	1299, 1706, 1871
Bse21I	CCTNAGG	1 cut(s)	257
Bse8I	GATNNNNATC	1 cut(s)	216
BseAI	TCCGGA	1 cut(s)	69
BseBI	CCWGG	1 cut(s)	534
BseCI	ATCGAT	1 cut(s)	543
BseDI	CCNNGG	4 cut(s)	35, 418, 581, 1540
BseJI	GATNNNNATC	1 cut(s)	216
BseLI	CCNNNNNNNGG	3 cut(s)	193, 424, 1546
BseMII	CTCAG	7 cut(s)	42, 271, 674, 722, 824, 1279, 1809
BseNI	ACTGG	3 cut(s)	1299, 1706, 1871
BseRI	GAGGAG	3 cut(s)	883, 1748, 1994
BseXI	GCAGC	6 cut(s)	27, 331, 1019, 1601, 1608, 1696
BsgI	GTGCAG	1 cut(s)	1703
BshFI	GGCC	1 cut(s)	1580
BshNI	GGYRCC	1 cut(s)	529
BshVI	ATCGAT	1 cut(s)	543
BsiHKAI	GWGCWC	2 cut(s)	69, 954
BsiHKCI	CYCGRG	1 cut(s)	1226
BsiSI	CCGG	4 cut(s)	70, 818, 947, 1311
BslFI	GGGAC	1 cut(s)	670
BslI	CCNNNNNNNGG	3 cut(s)	193, 424, 1546
BsmAI	GTCTC	4 cut(s)	31, 123, 1229, 1939
BsmFI	GGGAC	1 cut(s)	670
BsmI	GAATGC	2 cut(s)	18, 1753
BsnI	GGCC	1 cut(s)	1580
BsoBI	CYCGRG	1 cut(s)	1226
Bsp119I	TTCGAA	1 cut(s)	246
Bsp1286I	GDGCHC	2 cut(s)	69, 954
Bsp13I	TCCGGA	1 cut(s)	69
Bsp143I	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
Bsp1720I	GCTNAGC	1 cut(s)	1673
Bsp19I	CCATGG	3 cut(s)	35, 581, 1540
BspACI	CCGC	4 cut(s)	527, 1050, 1495, 1959
BspANI	GGCC	1 cut(s)	1580
BspCNI	CTCAG	7 cut(s)	41, 270, 673, 721, 825, 1278, 1810
BspDI	ATCGAT	1 cut(s)	543
BspEI	TCCGGA	1 cut(s)	69
BspLI	GGNNCC	2 cut(s)	531, 1072
BspMAI	CTGCAG	1 cut(s)	45
BspPI	GGATC	5 cut(s)	26, 175, 452, 1065, 1078
BspT104I	TTCGAA	1 cut(s)	246
BspT107I	GGYRCC	1 cut(s)	529
BspTI	CTTAAG	1 cut(s)	437
BsrI	ACTGG	3 cut(s)	1299, 1706, 1871
BssECI	CCNNGG	4 cut(s)	35, 418, 581, 1540
BssMI	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
BssNI	GRCGYC	1 cut(s)	682
BssT1I	CCWWGG	4 cut(s)	35, 418, 581, 1540
Bst2UI	CCWGG	1 cut(s)	534
Bst4CI	ACNGT	4 cut(s)	124, 643, 956, 1899
Bst6I	CTCTTC	4 cut(s)	95, 328, 792, 1188
BstACI	GRCGYC	1 cut(s)	682
BstAFI	CTTAAG	1 cut(s)	437
BstAPI	GCANNNNNTGC	2 cut(s)	974, 1492
BstBI	TTCGAA	1 cut(s)	246
BstC8I	GCNNGC	2 cut(s)	138, 1855
BstDEI	CTNAG	9 cut(s)	28, 257, 624, 660, 708, 833, 1265, 1673, 1818
BstDSI	CCRYGG	3 cut(s)	35, 581, 1540
BstENI	CCTNNNNNAGG	1 cut(s)	422
BstH2I	RGCGCY	1 cut(s)	1283
BstHHI	GCGC	1 cut(s)	1282
BstKTI	GATC	8 cut(s)	34, 170, 372, 460, 486, 519, 1073, 1894
BstMAI	GTCTC	4 cut(s)	31, 123, 1229, 1939
BstMBI	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
BstMWI	GCNNNNNNNGC	5 cut(s)	974, 1097, 1492, 1605, 1614
BstNI	CCWGG	1 cut(s)	534
BstNSI	RCATGY	1 cut(s)	1019
BstSCI	CCNGG	2 cut(s)	532, 1309
BstSFI	CTRYAG	1 cut(s)	41
BstV1I	GCAGC	6 cut(s)	27, 331, 1019, 1601, 1608, 1696
BstV2I	GAAGAC	2 cut(s)	1266, 1815
BstX2I	RGATCY	2 cut(s)	31, 1070
BstYI	RGATCY	2 cut(s)	31, 1070
Bsu15I	ATCGAT	1 cut(s)	543
Bsu36I	CCTNAGG	1 cut(s)	257
BsuRI	GGCC	1 cut(s)	1580
BsuTUI	ATCGAT	1 cut(s)	543
BtgI	CCRYGG	3 cut(s)	35, 581, 1540
BtsI	GCAGTG	1 cut(s)	288
BtsIMutI	CAGTG	2 cut(s)	288, 1292
Cac8I	GCNNGC	2 cut(s)	138, 1855
CfoI	GCGC	1 cut(s)	1282
Cfr13I	GGNCC	1 cut(s)	1578
ClaI	ATCGAT	1 cut(s)	543
CseI	GACGC	1 cut(s)	671
Csp6I	GTAC	6 cut(s)	10, 500, 572, 639, 1498, 1864
CviAII	CATG	7 cut(s)	36, 97, 284, 582, 1016, 1541, 1618
CviQI	GTAC	6 cut(s)	10, 500, 572, 639, 1498, 1864
DdeI	CTNAG	9 cut(s)	28, 257, 624, 660, 708, 833, 1265, 1673, 1818
DpnI	GATC	8 cut(s)	33, 169, 371, 459, 485, 518, 1072, 1893
DpnII	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
DraIII	CACNNNGTG	1 cut(s)	609
Eam1104I	CTCTTC	4 cut(s)	95, 328, 792, 1188
EarI	CTCTTC	4 cut(s)	95, 328, 792, 1188
Ecl136II	GAGCTC	1 cut(s)	67
Eco130I	CCWWGG	4 cut(s)	35, 418, 581, 1540
Eco24I	GRGCYC	1 cut(s)	69
Eco32I	GATATC	1 cut(s)	869
Eco53kI	GAGCTC	1 cut(s)	67
Eco57I	CTGAAG	2 cut(s)	1430, 1827
Eco81I	CCTNAGG	1 cut(s)	257
Eco88I	CYCGRG	1 cut(s)	1226
EcoICRI	GAGCTC	1 cut(s)	67
EcoNI	CCTNNNNNAGG	1 cut(s)	422
EcoRI	GAATTC	2 cut(s)	248, 1231
EcoRII	CCWGG	1 cut(s)	532
EcoRV	GATATC	1 cut(s)	869
EcoT14I	CCWWGG	4 cut(s)	35, 418, 581, 1540
EcoT38I	GRGCYC	1 cut(s)	69
ErhI	CCWWGG	4 cut(s)	35, 418, 581, 1540
FaeI	CATG	7 cut(s)	39, 100, 287, 585, 1019, 1544, 1621
FaqI	GGGAC	1 cut(s)	670
FatI	CATG	7 cut(s)	35, 96, 283, 581, 1015, 1540, 1617
FbaI	TGATCA	1 cut(s)	516
FblI	GTMKAC	1 cut(s)	811
Fnu4HI	GCNGC	6 cut(s)	41, 345, 1033, 1597, 1615, 1685
FriOI	GRGCYC	1 cut(s)	69
Fsp4HI	GCNGC	6 cut(s)	41, 345, 1033, 1597, 1615, 1685
FspBI	CTAG	4 cut(s)	23, 941, 1571, 1689
GlaI	GCGC	1 cut(s)	1281
GluI	GCNGC	6 cut(s)	41, 345, 1033, 1597, 1615, 1685
GsuI	CTGGAG	3 cut(s)	1171, 1307, 1723
HaeII	RGCGCY	1 cut(s)	1283
HaeIII	GGCC	1 cut(s)	1580
HapII	CCGG	4 cut(s)	70, 818, 947, 1311
HgaI	GACGC	1 cut(s)	671
HhaI	GCGC	1 cut(s)	1282
Hin1I	GRCGYC	1 cut(s)	682
Hin1II	CATG	7 cut(s)	39, 100, 287, 585, 1019, 1544, 1621
Hin6I	GCGC	1 cut(s)	1280
HinP1I	GCGC	1 cut(s)	1280
HincII	GTYRAC	1 cut(s)	778
HindII	GTYRAC	1 cut(s)	778
HpaII	CCGG	4 cut(s)	70, 818, 947, 1311
HphI	GGTGA	5 cut(s)	46, 917, 1299, 1708, 1834
Hpy166II	GTNNAC	5 cut(s)	54, 778, 812, 959, 1699
Hpy8I	GTNNAC	5 cut(s)	54, 778, 812, 959, 1699
Hpy99I	CGWCG	2 cut(s)	17, 1899
HpyAV	CCTTC	6 cut(s)	379, 668, 672, 898, 1061, 1540
HpyCH4III	ACNGT	4 cut(s)	124, 643, 956, 1899
HpyCH4IV	ACGT	2 cut(s)	12, 1251
HpyF10VI	GCNNNNNNNGC	5 cut(s)	974, 1097, 1492, 1605, 1614
HpyF3I	CTNAG	9 cut(s)	28, 257, 624, 660, 708, 833, 1265, 1673, 1818
HpySE526I	ACGT	2 cut(s)	12, 1251
Hsp92I	GRCGYC	1 cut(s)	682
Hsp92II	CATG	7 cut(s)	39, 100, 287, 585, 1019, 1544, 1621
HspAI	GCGC	1 cut(s)	1280
Kpn2I	TCCGGA	1 cut(s)	69
Ksp22I	TGATCA	1 cut(s)	516
Kzo9I	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
LmnI	GCTCC	4 cut(s)	64, 72, 72, 949
Lsp1109I	GCAGC	6 cut(s)	27, 331, 1019, 1601, 1608, 1696
LweI	GCATC	6 cut(s)	226, 398, 898, 964, 1482, 1549
MaeI	CTAG	4 cut(s)	23, 941, 1571, 1689
MaeII	ACGT	2 cut(s)	12, 1251
MaeIII	GTNAC	4 cut(s)	880, 1290, 1711, 1723
MalI	GATC	8 cut(s)	33, 169, 371, 459, 485, 518, 1072, 1893
MboI	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
MfeI	CAATTG	3 cut(s)	761, 851, 1487
MflI	RGATCY	2 cut(s)	31, 1070
MhlI	GDGCHC	2 cut(s)	69, 954
MlyI	GAGTC	6 cut(s)	652, 817, 1119, 1298, 1949, 1976
MmeI	TCCRAC	4 cut(s)	106, 613, 1534, 1626
MroI	TCCGGA	1 cut(s)	69
MroXI	GAANNNNTTC	1 cut(s)	50
MseI	TTAA	6 cut(s)	438, 576, 1083, 1350, 1466, 1629
MslI	CAYNNNNRTG	3 cut(s)	59, 1119, 1539
MspCI	CTTAAG	1 cut(s)	437
MspI	CCGG	4 cut(s)	70, 818, 947, 1311
MspR9I	CCNGG	2 cut(s)	534, 1311
MunI	CAATTG	3 cut(s)	761, 851, 1487
Mva1269I	GAATGC	2 cut(s)	18, 1753
MvaI	CCWGG	1 cut(s)	534
MwoI	GCNNNNNNNGC	5 cut(s)	974, 1097, 1492, 1605, 1614
NciI	CCSGG	1 cut(s)	1311
NcoI	CCATGG	3 cut(s)	35, 581, 1540
NdeII	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
NlaIII	CATG	7 cut(s)	39, 100, 287, 585, 1019, 1544, 1621
NlaIV	GGNNCC	2 cut(s)	531, 1072
NmuCI	GTSAC	2 cut(s)	880, 1290
NspI	RCATGY	1 cut(s)	1019
NspV	TTCGAA	1 cut(s)	246
OliI	CACNNNNGTG	1 cut(s)	59
PcsI	WCGNNNNNNNCGW	1 cut(s)	786
PctI	GAATGC	2 cut(s)	18, 1753
PdmI	GAANNNNTTC	1 cut(s)	50
PfeI	GAWTC	6 cut(s)	46, 377, 982, 1078, 1825, 1837
PkrI	GCNGC	6 cut(s)	42, 346, 1034, 1598, 1616, 1686
PleI	GAGTC	6 cut(s)	652, 816, 1119, 1297, 1949, 1975
PpsI	GAGTC	6 cut(s)	652, 816, 1119, 1297, 1949, 1975
Psp124BI	GAGCTC	1 cut(s)	69
Psp6I	CCWGG	1 cut(s)	532
PspGI	CCWGG	1 cut(s)	532
PspN4I	GGNNCC	2 cut(s)	531, 1072
PspPI	GGNCC	1 cut(s)	1578
PstI	CTGCAG	1 cut(s)	45
PsuI	RGATCY	2 cut(s)	31, 1070
RsaI	GTAC	6 cut(s)	11, 501, 573, 640, 1499, 1865
RsaNI	GTAC	6 cut(s)	10, 500, 572, 639, 1498, 1864
RseI	CAYNNNNRTG	3 cut(s)	59, 1119, 1539
SacI	GAGCTC	1 cut(s)	69
SaqAI	TTAA	6 cut(s)	438, 576, 1083, 1350, 1466, 1629
SatI	GCNGC	6 cut(s)	41, 345, 1033, 1597, 1615, 1685
Sau3AI	GATC	8 cut(s)	31, 167, 369, 457, 483, 516, 1070, 1891
Sau96I	GGNCC	1 cut(s)	1578
ScaI	AGTACT	2 cut(s)	501, 573
SchI	GAGTC	6 cut(s)	652, 817, 1119, 1298, 1949, 1976
ScrFI	CCNGG	2 cut(s)	534, 1311
SduI	GDGCHC	2 cut(s)	69, 954
SfaNI	GCATC	6 cut(s)	226, 398, 898, 964, 1482, 1549
SfcI	CTRYAG	1 cut(s)	41
SfuI	TTCGAA	1 cut(s)	246
SmiMI	CAYNNNNRTG	3 cut(s)	59, 1119, 1539
SmlI	CTYRAG	5 cut(s)	155, 338, 437, 1525, 1664
SmoI	CTYRAG	5 cut(s)	155, 338, 437, 1525, 1664
SsiI	CCGC	4 cut(s)	527, 1050, 1495, 1959
SspI	AATATT	2 cut(s)	652, 1429
SspMI	CTAG	4 cut(s)	23, 941, 1571, 1689
SstI	GAGCTC	1 cut(s)	69
StyD4I	CCNGG	2 cut(s)	532, 1309
StyI	CCWWGG	4 cut(s)	35, 418, 581, 1540
TaaI	ACNGT	4 cut(s)	124, 643, 956, 1899
TaiI	ACGT	2 cut(s)	15, 1254
TaqI	TCGA	8 cut(s)	228, 240, 246, 480, 543, 1790, 1887, 1894
TatI	WGTACW	4 cut(s)	499, 571, 638, 1863
TfiI	GAWTC	6 cut(s)	46, 377, 982, 1078, 1825, 1837
Tru1I	TTAA	6 cut(s)	438, 576, 1083, 1350, 1466, 1629
Tru9I	TTAA	6 cut(s)	438, 576, 1083, 1350, 1466, 1629
TscAI	CASTG	2 cut(s)	295, 1299
TseFI	GTSAC	2 cut(s)	880, 1290
TseI	GCWGC	6 cut(s)	40, 344, 1032, 1596, 1614, 1684
Tsp45I	GTSAC	2 cut(s)	880, 1290
TspGWI	ACGGA	1 cut(s)	1991
TspRI	CASTG	2 cut(s)	295, 1299
Vha464I	CTTAAG	1 cut(s)	437
XagI	CCTNNNNNAGG	1 cut(s)	422
XapI	RAATTY	4 cut(s)	248, 844, 1231, 1653
XbaI	TCTAGA	1 cut(s)	1570
XceI	RCATGY	1 cut(s)	1019
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1118
XmiI	GTMKAC	1 cut(s)	811
XmnI	GAANNNNTTC	1 cut(s)	50
XspI	CTAG	4 cut(s)	23, 941, 1571, 1689
ZrmI	AGTACT	2 cut(s)	501, 573

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.