AT5G49800

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0016207
Physical Location & Seq
Reverse (-)
20234509 .. 20236329
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G49800.2

Sequence Viewer

Length: 729 bp
ATGAACGGTCCAGTCCCCTCCAGCCGCTCCTGGTCGGTGAGCGAGGAGTCGCTGAGAAGGTACGTGAGGTTCGCGAGTGAGAGCTGCATCCAAGAGCTCTTATCTTCCTCTGAGGCCGGACGCTTTGGGAACGCTTCCAATGGGTGGAAGATGGTGAGGCATGACTCGAACGGTGTCGAGATTTCAAAACGCGACTCGGGATCGCTACACGCGTTTCGAAGCCGCAGGATCCTCACATCAGTCTCCCCAGAGCAGTTCATCACGGTTGCAAATGCGATAGACGCGGCCAAGCAATGGGAAGGCGACCTGGTGGAGGCAACACACATTAGAGACATAGATGAAAATCTGAGCGTAATCCGTTTAAGGTTTGGCGAAAACTCGAAACCGCTCTTTAGGAACAGAGAGTTCATTATCTACGAAAGACGTGAGACCATGCAAGACGGTACTCTCGTGGTGGCGGTTGCGTCGCTACCAAAGGAGATGGCAGAAGGATTGGAGCCAACAGAGAAAAAGAAAAACAATTATATTAGAGGGTTTTTGGTTGAGTCGGGTTGGGTTTTGGAGAAGCTTGAAGATACCTCTTGCATGATTACTTATGTTGTTCAGTTGGATCCTGCAGGATGGTTGCCAAAGTGTTTTGTGAACAGACTAAACACAAAGTTAGTAATGATAATTGATAACCTTAGAAAGGTTGCTCAAGCTTATCTACCCTCTTTTCCTCCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

242

Amino Acids

27.42

Weight (kDa)

7.71

Isoelectric Point (pI)

58.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 45 - 203 2.3e-08 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49800 AT5G49800
fragaria_vesca FvH4_5g27180
malus_domestica MD08G1250400.v1.1
prunus_persica Prupe.1G587600_v2.0.a1
pyrus_communis pycom08g21720
rosa_chinensis RchiOBHm_Chr7g0218271
rosa_laevigata RLG00000002383
rosa_multiflora Rmu_sc0004956.1_g000009
rosa_roxburghii Rroxscaffold_3G00241140
rosa_rugosa Rorug07G0176000
rosa_samantha Rh7AG317700 Rh7BG309000 Rh7DG316600
rosa_wichuraiana Rw7G026970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 144, 294
AccBSI CCGCTC 2 cut(s) 27, 388
AccII CGCG 4 cut(s) 74, 192, 212, 284
AciI CCGC 5 cut(s) 25, 223, 284, 386, 458
AclWI GGATC 5 cut(s) 208, 223, 236, 605, 618
AcoI YGGCCR 1 cut(s) 285
AfaI GTAC 2 cut(s) 62, 445
AfiI CCNNNNNNNGG 4 cut(s) 144, 294, 313, 688
AflIII ACRYGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 186, 572
AjiI CACGTC 1 cut(s) 425
AjnI CCWGG 2 cut(s) 29, 306
AluBI AGCT 4 cut(s) 84, 97, 568, 701
AluI AGCT 4 cut(s) 84, 97, 568, 701
Alw21I GWGCWC 1 cut(s) 99
Alw26I GTCTC 3 cut(s) 247, 324, 422
AlwI GGATC 5 cut(s) 208, 223, 236, 605, 618
Ama87I CYCGRG 1 cut(s) 196
AoxI GGCC 2 cut(s) 114, 285
ApeKI GCWGC 1 cut(s) 84
AspS9I GGNCC 1 cut(s) 8
AsuHPI GGTGA 2 cut(s) 49, 166
AsuII TTCGAA 1 cut(s) 217
AvaI CYCGRG 1 cut(s) 196
AvaII GGWCC 1 cut(s) 8
BaeI ACNNNNGTAYC 2 cut(s) 435, 468
BamHI GGATCC 2 cut(s) 228, 610
BanII GRGCYC 1 cut(s) 99
BauI CACGAG 1 cut(s) 449
Bbv12I GWGCWC 1 cut(s) 99
BbvI GCAGC 1 cut(s) 71
BccI CCATC 3 cut(s) 145, 475, 615
BciT130I CCWGG 2 cut(s) 31, 308
BcoDI GTCTC 3 cut(s) 247, 324, 422
BfmI CTRYAG 1 cut(s) 615
BisI GCNGC 4 cut(s) 25, 85, 223, 285
BlsI GCNGC 4 cut(s) 26, 86, 224, 286
Bme1390I CCNGG 2 cut(s) 31, 308
Bme18I GGWCC 1 cut(s) 8
BmeT110I CYCGRG 1 cut(s) 196
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 8
BmiI GGNNCC 3 cut(s) 230, 498, 612
BmrFI CCNGG 2 cut(s) 31, 308
BmsI GCATC 1 cut(s) 96
BpmI CTGGAG 1 cut(s) 4
Bpu14I TTCGAA 1 cut(s) 217
BpuEI CTTGAG 1 cut(s) 681
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 342
BsaI GGTCTC 1 cut(s) 422
Bsc4I CCNNNNNNNGG 4 cut(s) 144, 294, 313, 688
Bse1I ACTGG 1 cut(s) 11
Bse3DI GCAATG 1 cut(s) 299
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 2 cut(s) 31, 308
BseGI GGATG 2 cut(s) 87, 626
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 4 cut(s) 144, 294, 313, 688
BseMI GCAATG 1 cut(s) 299
BseMII CTCAG 3 cut(s) 44, 102, 338
BseNI ACTGG 1 cut(s) 11
BseRI GAGGAG 2 cut(s) 59, 711
BseXI GCAGC 1 cut(s) 71
Bsh1236I CGCG 4 cut(s) 74, 192, 212, 284
BshFI GGCC 2 cut(s) 116, 287
BsiHKAI GWGCWC 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 196
BsiSI CCGG 1 cut(s) 117
BslI CCNNNNNNNGG 4 cut(s) 144, 294, 313, 688
BsmAI GTCTC 3 cut(s) 247, 324, 422
BsnI GGCC 2 cut(s) 116, 287
Bso31I GGTCTC 1 cut(s) 422
BsoBI CYCGRG 1 cut(s) 196
Bsp119I TTCGAA 1 cut(s) 217
Bsp1286I GDGCHC 1 cut(s) 99
Bsp143I GATC 3 cut(s) 200, 228, 610
Bsp68I TCGCGA 1 cut(s) 74
BspACI CCGC 5 cut(s) 25, 223, 284, 386, 458
BspANI GGCC 2 cut(s) 116, 287
BspCNI CTCAG 3 cut(s) 45, 103, 339
BspFNI CGCG 4 cut(s) 74, 192, 212, 284
BspLI GGNNCC 3 cut(s) 230, 498, 612
BspMAI CTGCAG 1 cut(s) 619
BspPI GGATC 5 cut(s) 208, 223, 236, 605, 618
BspT104I TTCGAA 1 cut(s) 217
BspTNI GGTCTC 1 cut(s) 422
BsrBI CCGCTC 2 cut(s) 27, 388
BsrDI GCAATG 1 cut(s) 299
BsrI ACTGG 1 cut(s) 11
BssMI GATC 3 cut(s) 200, 228, 610
BssSI CACGAG 1 cut(s) 449
Bst2BI CACGAG 1 cut(s) 449
Bst2UI CCWGG 2 cut(s) 31, 308
Bst4CI ACNGT 4 cut(s) 8, 173, 265, 443
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 217
BstDEI CTNAG 5 cut(s) 53, 111, 347, 683, 726
BstENI CCTNNNNNAGG 2 cut(s) 311, 686
BstF5I GGATG 2 cut(s) 87, 626
BstFNI CGCG 4 cut(s) 74, 192, 212, 284
BstKTI GATC 3 cut(s) 203, 231, 613
BstMAI GTCTC 3 cut(s) 247, 324, 422
BstMBI GATC 3 cut(s) 200, 228, 610
BstMWI GCNNNNNNNGC 1 cut(s) 281
BstNI CCWGG 2 cut(s) 31, 308
BstSCI CCNGG 2 cut(s) 29, 306
BstSFI CTRYAG 1 cut(s) 615
BstUI CGCG 4 cut(s) 74, 192, 212, 284
BstV1I GCAGC 1 cut(s) 71
BstX2I RGATCY 2 cut(s) 228, 610
BstYI RGATCY 2 cut(s) 228, 610
BsuRI GGCC 2 cut(s) 116, 287
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 2 cut(s) 87, 626
BtuMI TCGCGA 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 8
CseI GACGC 3 cut(s) 129, 290, 453
CsiI ACCWGGT 1 cut(s) 306
Csp6I GTAC 2 cut(s) 61, 444
CviAII CATG 3 cut(s) 161, 433, 586
CviJI RGCY 9 cut(s) 24, 84, 97, 116, 222, 287, 499, 568, 701
CviKI_1 RGCY 9 cut(s) 24, 84, 97, 116, 222, 287, 499, 568, 701
CviQI GTAC 2 cut(s) 61, 444
DdeI CTNAG 5 cut(s) 53, 111, 347, 683, 726
DpnI GATC 3 cut(s) 202, 230, 612
DpnII GATC 3 cut(s) 200, 228, 610
EaeI YGGCCR 1 cut(s) 285
Ecl136II GAGCTC 1 cut(s) 97
Eco24I GRGCYC 1 cut(s) 99
Eco31I GGTCTC 1 cut(s) 422
Eco47I GGWCC 1 cut(s) 8
Eco53kI GAGCTC 1 cut(s) 97
Eco88I CYCGRG 1 cut(s) 196
EcoICRI GAGCTC 1 cut(s) 97
EcoNI CCTNNNNNAGG 2 cut(s) 311, 686
EcoRII CCWGG 2 cut(s) 29, 306
EcoT38I GRGCYC 1 cut(s) 99
FaeI CATG 3 cut(s) 164, 436, 589
FaiI YATR 6 cut(s) 162, 335, 434, 525, 587, 597
FatI CATG 3 cut(s) 160, 432, 585
Fnu4HI GCNGC 4 cut(s) 25, 85, 223, 285
FokI GGATG 2 cut(s) 74, 633
FriOI GRGCYC 1 cut(s) 99
Fsp4HI GCNGC 4 cut(s) 25, 85, 223, 285
GluI GCNGC 4 cut(s) 25, 85, 223, 285
GsuI CTGGAG 1 cut(s) 4
HaeIII GGCC 2 cut(s) 116, 287
HapII CCGG 1 cut(s) 117
HgaI GACGC 3 cut(s) 129, 290, 453
Hin1II CATG 3 cut(s) 164, 436, 589
HindIII AAGCTT 2 cut(s) 566, 699
HinfI GANTC 4 cut(s) 47, 164, 194, 545
HpaII CCGG 1 cut(s) 117
HphI GGTGA 2 cut(s) 49, 166
Hpy166II GTNNAC 1 cut(s) 643
Hpy188I TCNGA 2 cut(s) 112, 348
Hpy188III TCNNGA 3 cut(s) 73, 178, 198
Hpy8I GTNNAC 1 cut(s) 643
Hpy99I CGWCG 1 cut(s) 469
HpyAV CCTTC 3 cut(s) 51, 293, 482
HpyCH4III ACNGT 4 cut(s) 8, 173, 265, 443
HpyCH4IV ACGT 2 cut(s) 63, 424
HpyCH4V TGCA 5 cut(s) 87, 269, 436, 585, 617
HpyF10VI GCNNNNNNNGC 1 cut(s) 281
HpyF3I CTNAG 5 cut(s) 53, 111, 347, 683, 726
HpySE526I ACGT 2 cut(s) 63, 424
Hsp92II CATG 3 cut(s) 164, 436, 589
Kzo9I GATC 3 cut(s) 200, 228, 610
LmnI GCTCC 2 cut(s) 32, 496
Lsp1109I GCAGC 1 cut(s) 71
LweI GCATC 1 cut(s) 96
MabI ACCWGGT 1 cut(s) 306
MaeII ACGT 2 cut(s) 63, 424
MalI GATC 3 cut(s) 202, 230, 612
MbiI CCGCTC 2 cut(s) 27, 388
MboI GATC 3 cut(s) 200, 228, 610
MboII GAAGA 3 cut(s) 96, 160, 584
MflI RGATCY 2 cut(s) 228, 610
MhlI GDGCHC 1 cut(s) 99
MluCI AATT 2 cut(s) 520, 672
MluI ACGCGT 1 cut(s) 210
MlyI GAGTC 4 cut(s) 56, 158, 188, 554
MmeI TCCRAC 1 cut(s) 588
MseI TTAA 1 cut(s) 362
MspI CCGG 1 cut(s) 117
MspR9I CCNGG 2 cut(s) 31, 308
MvaI CCWGG 2 cut(s) 31, 308
MvnI CGCG 4 cut(s) 74, 192, 212, 284
MwoI GCNNNNNNNGC 1 cut(s) 281
NdeII GATC 3 cut(s) 200, 228, 610
NlaIII CATG 3 cut(s) 164, 436, 589
NlaIV GGNNCC 3 cut(s) 230, 498, 612
NruI TCGCGA 1 cut(s) 74
NspV TTCGAA 1 cut(s) 217
PcsI WCGNNNNNNNCGW 2 cut(s) 209, 447
PflMI CCANNNNNTGG 2 cut(s) 144, 294
PkrI GCNGC 4 cut(s) 26, 86, 224, 286
PleI GAGTC 4 cut(s) 55, 158, 188, 553
PpsI GAGTC 4 cut(s) 55, 158, 188, 553
Ppu21I YACGTR 1 cut(s) 64
Psp124BI GAGCTC 1 cut(s) 99
Psp6I CCWGG 2 cut(s) 29, 306
PspGI CCWGG 2 cut(s) 29, 306
PspN4I GGNNCC 3 cut(s) 230, 498, 612
PspPI GGNCC 1 cut(s) 8
PsrI GAACNNNNNNTAC 2 cut(s) 53, 85
PstI CTGCAG 1 cut(s) 619
PsuI RGATCY 2 cut(s) 228, 610
RruI TCGCGA 1 cut(s) 74
RsaI GTAC 2 cut(s) 62, 445
RsaNI GTAC 2 cut(s) 61, 444
SacI GAGCTC 1 cut(s) 99
SaqAI TTAA 1 cut(s) 362
SatI GCNGC 4 cut(s) 25, 85, 223, 285
Sau3AI GATC 3 cut(s) 200, 228, 610
Sau96I GGNCC 1 cut(s) 8
SbfI CCTGCAGG 1 cut(s) 619
SchI GAGTC 4 cut(s) 56, 158, 188, 554
ScrFI CCNGG 2 cut(s) 31, 308
SdaI CCTGCAGG 1 cut(s) 619
SduI GDGCHC 1 cut(s) 99
SexAI ACCWGGT 1 cut(s) 306
SfaNI GCATC 1 cut(s) 96
SfcI CTRYAG 1 cut(s) 615
SfuI TTCGAA 1 cut(s) 217
SinI GGWCC 1 cut(s) 8
SmlI CTYRAG 1 cut(s) 696
SmoI CTYRAG 1 cut(s) 696
Sse8387I CCTGCAGG 1 cut(s) 619
Sse9I AATT 2 cut(s) 520, 672
SsiI CCGC 5 cut(s) 25, 223, 284, 386, 458
SstI GAGCTC 1 cut(s) 99
StyD4I CCNGG 2 cut(s) 29, 306
TaaI ACNGT 4 cut(s) 8, 173, 265, 443
TaiI ACGT 2 cut(s) 66, 427
TaqI TCGA 4 cut(s) 167, 177, 217, 380
TasI AATT 2 cut(s) 520, 672
TauI GCSGC 3 cut(s) 27, 225, 287
Tru1I TTAA 1 cut(s) 362
Tru9I TTAA 1 cut(s) 362
TseI GCWGC 1 cut(s) 84
TspDTI ATGAA 4 cut(s) 17, 247, 354, 397
TspGWI ACGGA 1 cut(s) 347
Van91I CCANNNNNTGG 2 cut(s) 144, 294
VpaK11BI GGWCC 1 cut(s) 8
XagI CCTNNNNNAGG 2 cut(s) 311, 686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.