MD08G1250400.v1.1

START domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0016207
Physical Location & Seq
Forward (+)
31516462 .. 31518167
1706 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1250400.v1.1.491

Sequence Viewer

Length: 744 bp
ATGACCACTACTAGTCCAGCACCTTGCAGCAGCAGCACCACCACCACCAGCAGCCGTTCCTGGTCCATAAGTGAGGACTCGCTTAAAAGGTATGTGCAGTTTGCAAGCGAAAGCTGCATTCAAGAATTACTGTCTGCTTCCGACTCCAACAGACCCAACGACGGCTGGAAACCCCTCCTTACTCTTGGCAACGGAGTTGAGATCTCCAAACGCAGGTCTGGATCCTTTCACACCTTCCGCAGCCGCTGCCTCCTCACTTCTCTGTCACCCCAGCAATTCATTACCGTTGCAAACGCCATTGATGCTGCCAAGCAATGGGATCCTCATCTTGTAGAAGCAAGGTACATAAAAGATCTTGGAGATAACCTCAGCATCATCCGTCTCAGGTTTGGAGATCATTCAAAGCCCCTCTTTCGAAACAGAGAATTCGTAGTCTACGAGCGACGTGAAACCATGGAAGATGGCACACTGGTGGTAGCAGTTGCTTCACTTCCAAAGGAGATAGCTGCCGGATTGGAACCAAAGCAAAATAACGCCATCAGGGGCCTGCTGCTGCAATCAGGATGGGTTGTTGATAAGCTTCAAGGTCACTCCTGCATGGTTACCTATGTTGTTCAGTTAGATCCTGCCGGATGGATGCCGAGGTGCTTTGTCAATCGACATAACAACAGACTCGTTATGATCATTGAAAATCTAAAGAAACTGGCACAAGCCTCTCCTCCTACTGATGCCCAAACATCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
START

Protein Analysis

248

Amino Acids

27.55

Weight (kDa)

9.02

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
START PF01852 87 - 220 1e-11 START domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016207)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G49800 AT5G49800
fragaria_vesca FvH4_5g27180
malus_domestica MD08G1250400.v1.1
prunus_persica Prupe.1G587600_v2.0.a1
pyrus_communis pycom08g21720
rosa_chinensis RchiOBHm_Chr7g0218271
rosa_laevigata RLG00000002383
rosa_multiflora Rmu_sc0004956.1_g000009
rosa_roxburghii Rroxscaffold_3G00241140
rosa_rugosa Rorug07G0176000
rosa_samantha Rh7AG317700 Rh7BG309000 Rh7DG316600
rosa_wichuraiana Rw7G026970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 204
AccB7I CCANNNNNTGG 1 cut(s) 315
AccI GTMKAC 1 cut(s) 435
AciI CCGC 2 cut(s) 238, 244
AclWI GGATC 5 cut(s) 216, 229, 314, 327, 617
AcsI RAATTY 1 cut(s) 425
AfaI GTAC 1 cut(s) 344
AfiI CCNNNNNNNGG 3 cut(s) 161, 213, 315
AgsI TTSAA 4 cut(s) 122, 402, 584, 689
AhlI ACTAGT 1 cut(s) 11
AjiI CACGTC 1 cut(s) 446
AjnI CCWGG 1 cut(s) 59
AleI CACNNNNGTG 1 cut(s) 470
AluBI AGCT 3 cut(s) 114, 506, 580
AluI AGCT 3 cut(s) 114, 506, 580
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 5 cut(s) 216, 229, 314, 327, 617
AlwNI CAGNNNCTG 1 cut(s) 246
AoxI GGCC 1 cut(s) 544
ApoI RAATTY 1 cut(s) 425
AspS9I GGNCC 2 cut(s) 63, 544
AsuHPI GGTGA 1 cut(s) 258
AsuII TTCGAA 1 cut(s) 415
AvaII GGWCC 1 cut(s) 63
BamHI GGATCC 2 cut(s) 221, 319
BbvCI CCTCAGC 1 cut(s) 368
BccI CCATC 4 cut(s) 455, 545, 558, 627
BceAI ACGGC 2 cut(s) 39, 178
BciT130I CCWGG 1 cut(s) 61
BclI TGATCA 1 cut(s) 681
BcoDI GTCTC 1 cut(s) 386
BcuI ACTAGT 1 cut(s) 11
BfaI CTAG 1 cut(s) 12
BfuAI ACCTGC 1 cut(s) 204
BglII AGATCT 2 cut(s) 201, 352
Bme1390I CCNGG 1 cut(s) 61
Bme18I GGWCC 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 446
BmgT120I GGNCC 2 cut(s) 63, 544
BmiI GGNNCC 4 cut(s) 223, 321, 519, 545
BmrFI CCNGG 1 cut(s) 61
BmsI GCATC 4 cut(s) 292, 381, 627, 718
BplI GAGNNNNNCTC 2 cut(s) 351, 383
Bpu10I CCTNAGC 1 cut(s) 368
Bpu14I TTCGAA 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 324
BsaJI CCNNGG 2 cut(s) 453, 641
Bsc4I CCNNNNNNNGG 3 cut(s) 161, 213, 315
Bse1I ACTGG 2 cut(s) 474, 708
Bse3DI GCAATG 1 cut(s) 320
Bse8I GATNNNNATC 1 cut(s) 324
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 453, 641
BseGI GGATG 5 cut(s) 375, 569, 638, 642, 737
BseJI GATNNNNATC 1 cut(s) 324
BseLI CCNNNNNNNGG 3 cut(s) 161, 213, 315
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 2 cut(s) 382, 397
BseNI ACTGG 2 cut(s) 474, 708
BseRI GAGGAG 2 cut(s) 242, 708
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 546
BsiSI CCGG 2 cut(s) 510, 630
BslI CCNNNNNNNGG 3 cut(s) 161, 213, 315
BsmAI GTCTC 1 cut(s) 386
BsmBI CGTCTC 1 cut(s) 386
BsmI GAATGC 1 cut(s) 117
BsnI GGCC 1 cut(s) 546
Bsp119I TTCGAA 1 cut(s) 415
Bsp143I GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
Bsp19I CCATGG 1 cut(s) 453
BspACI CCGC 2 cut(s) 238, 244
BspANI GGCC 1 cut(s) 546
BspCNI CTCAG 2 cut(s) 381, 396
BspLI GGNNCC 4 cut(s) 223, 321, 519, 545
BspMI ACCTGC 1 cut(s) 204
BspPI GGATC 5 cut(s) 216, 229, 314, 327, 617
BspT104I TTCGAA 1 cut(s) 415
BsrDI GCAATG 1 cut(s) 320
BsrI ACTGG 2 cut(s) 474, 708
BssECI CCNNGG 2 cut(s) 453, 641
BssMI GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
BssT1I CCWWGG 1 cut(s) 453
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 2 cut(s) 132, 286
BstAPI GCANNNNNTGC 1 cut(s) 246
BstBI TTCGAA 1 cut(s) 415
BstC8I GCNNGC 2 cut(s) 106, 548
BstDEI CTNAG 2 cut(s) 368, 383
BstDSI CCRYGG 1 cut(s) 453
BstEII GGTNACC 1 cut(s) 601
BstF5I GGATG 5 cut(s) 375, 569, 638, 642, 737
BstKTI GATC 7 cut(s) 204, 224, 322, 355, 397, 625, 684
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
BstMWI GCNNNNNNNGC 4 cut(s) 33, 114, 246, 302
BstNI CCWGG 1 cut(s) 61
BstPI GGTNACC 1 cut(s) 601
BstSCI CCNGG 1 cut(s) 59
BstX2I RGATCY 5 cut(s) 201, 221, 319, 352, 622
BstYI RGATCY 5 cut(s) 201, 221, 319, 352, 622
BsuRI GGCC 1 cut(s) 546
BtgI CCRYGG 1 cut(s) 453
BtrI CACGTC 1 cut(s) 446
BtsCI GGATG 5 cut(s) 375, 569, 638, 642, 737
BtsIMutI CAGTG 1 cut(s) 467
BveI ACCTGC 1 cut(s) 204
Cac8I GCNNGC 2 cut(s) 106, 548
CaiI CAGNNNCTG 1 cut(s) 246
Cfr13I GGNCC 2 cut(s) 63, 544
Csp6I GTAC 1 cut(s) 343
CviAII CATG 2 cut(s) 454, 598
CviJI RGCY 9 cut(s) 54, 114, 165, 243, 406, 506, 546, 580, 713
CviKI_1 RGCY 9 cut(s) 54, 114, 165, 243, 406, 506, 546, 580, 713
CviQI GTAC 1 cut(s) 343
DdeI CTNAG 2 cut(s) 368, 383
DpnI GATC 7 cut(s) 203, 223, 321, 354, 396, 624, 683
DpnII GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
Eco130I CCWWGG 1 cut(s) 453
Eco47I GGWCC 1 cut(s) 63
Eco91I GGTNACC 1 cut(s) 601
EcoO109I RGGNCCY 1 cut(s) 544
EcoO65I GGTNACC 1 cut(s) 601
EcoRI GAATTC 1 cut(s) 425
EcoRII CCWGG 1 cut(s) 59
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
Esp3I CGTCTC 1 cut(s) 386
FaeI CATG 2 cut(s) 457, 601
FaiI YATR 8 cut(s) 68, 93, 347, 455, 599, 609, 663, 680
FalI AAGNNNNNCTT 2 cut(s) 395, 427
FatI CATG 2 cut(s) 453, 597
FbaI TGATCA 1 cut(s) 681
FblI GTMKAC 1 cut(s) 435
FokI GGATG 5 cut(s) 362, 576, 645, 649, 724
FspBI CTAG 1 cut(s) 12
GsaI CCCAGC 1 cut(s) 274
HaeIII GGCC 1 cut(s) 546
HapII CCGG 2 cut(s) 510, 630
Hin1II CATG 2 cut(s) 457, 601
HindIII AAGCTT 1 cut(s) 578
HinfI GANTC 3 cut(s) 77, 143, 672
HpaII CCGG 2 cut(s) 510, 630
HphI GGTGA 1 cut(s) 258
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 1 cut(s) 142
Hpy188III TCNNGA 4 cut(s) 122, 219, 561, 741
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 2 cut(s) 164, 447
HpyAV CCTTC 1 cut(s) 244
HpyCH4III ACNGT 2 cut(s) 132, 286
HpyCH4IV ACGT 1 cut(s) 445
HpyCH4V TGCA 7 cut(s) 27, 97, 104, 117, 290, 556, 597
HpyF10VI GCNNNNNNNGC 4 cut(s) 33, 114, 246, 302
HpyF3I CTNAG 2 cut(s) 368, 383
HpySE526I ACGT 1 cut(s) 445
Hsp92II CATG 2 cut(s) 457, 601
Ksp22I TGATCA 1 cut(s) 681
Kzo9I GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
LweI GCATC 4 cut(s) 292, 381, 627, 718
MaeI CTAG 1 cut(s) 12
MaeII ACGT 1 cut(s) 445
MaeIII GTNAC 3 cut(s) 264, 587, 601
MalI GATC 7 cut(s) 203, 223, 321, 354, 396, 624, 683
MboI GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
MboII GAAGA 1 cut(s) 470
MflI RGATCY 5 cut(s) 201, 221, 319, 352, 622
MluCI AATT 3 cut(s) 125, 275, 425
MlyI GAGTC 3 cut(s) 71, 137, 666
MmeI TCCRAC 2 cut(s) 165, 171
MseI TTAA 1 cut(s) 84
MslI CAYNNNNRTG 1 cut(s) 470
MspA1I CMGCKG 1 cut(s) 246
MspI CCGG 2 cut(s) 510, 630
MspR9I CCNGG 1 cut(s) 61
Mva1269I GAATGC 1 cut(s) 117
MvaI CCWGG 1 cut(s) 61
MwoI GCNNNNNNNGC 4 cut(s) 33, 114, 246, 302
NcoI CCATGG 1 cut(s) 453
NdeII GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
NlaIII CATG 2 cut(s) 457, 601
NlaIV GGNNCC 4 cut(s) 223, 321, 519, 545
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 2 cut(s) 264, 587
NspV TTCGAA 1 cut(s) 415
OliI CACNNNNGTG 1 cut(s) 470
PcsI WCGNNNNNNNCGW 1 cut(s) 435
PctI GAATGC 1 cut(s) 117
PflMI CCANNNNNTGG 1 cut(s) 315
PleI GAGTC 3 cut(s) 71, 137, 666
PpsI GAGTC 3 cut(s) 71, 137, 666
Psp6I CCWGG 1 cut(s) 59
PspEI GGTNACC 1 cut(s) 601
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 59
PspN4I GGNNCC 4 cut(s) 223, 321, 519, 545
PspPI GGNCC 2 cut(s) 63, 544
PstNI CAGNNNCTG 1 cut(s) 246
PsuI RGATCY 5 cut(s) 201, 221, 319, 352, 622
RsaI GTAC 1 cut(s) 344
RsaNI GTAC 1 cut(s) 343
RseI CAYNNNNRTG 1 cut(s) 470
SaqAI TTAA 1 cut(s) 84
Sau3AI GATC 7 cut(s) 201, 221, 319, 352, 394, 622, 681
Sau96I GGNCC 2 cut(s) 63, 544
SchI GAGTC 3 cut(s) 71, 137, 666
ScrFI CCNGG 1 cut(s) 61
SfaNI GCATC 4 cut(s) 292, 381, 627, 718
SfuI TTCGAA 1 cut(s) 415
SinI GGWCC 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 470
SpeI ACTAGT 1 cut(s) 11
Sse9I AATT 3 cut(s) 125, 275, 425
SsiI CCGC 2 cut(s) 238, 244
SspMI CTAG 1 cut(s) 12
StyD4I CCNGG 1 cut(s) 59
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 2 cut(s) 132, 286
TaiI ACGT 1 cut(s) 448
TaqI TCGA 2 cut(s) 415, 658
TasI AATT 3 cut(s) 125, 275, 425
TauI GCSGC 1 cut(s) 246
Tru1I TTAA 1 cut(s) 84
Tru9I TTAA 1 cut(s) 84
TscAI CASTG 1 cut(s) 474
TseFI GTSAC 2 cut(s) 264, 587
Tsp45I GTSAC 2 cut(s) 264, 587
TspDTI ATGAA 1 cut(s) 268
TspGWI ACGGA 2 cut(s) 207, 368
TspRI CASTG 1 cut(s) 474
Van91I CCANNNNNTGG 1 cut(s) 315
VpaK11BI GGWCC 1 cut(s) 63
XapI RAATTY 1 cut(s) 425
XmiI GTMKAC 1 cut(s) 435
XspI CTAG 1 cut(s) 12
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.