AT5G51670

Domain of unknown function (DUF3475)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014490
Physical Location & Seq
Forward (+)
20993424 .. 20995644
2221 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51670.2

Sequence Viewer

Length: 1425 bp
ATGGCTCTCGAAACTTTTCTAATAAAACTCAAAAACGCTATTTCCTCCAAACCAACTTCTCGTCGACCACACCGATCATCTCCTCCGATATCAACCACCACTTCCTCCGTCGGAGTATTATCGTTCGAAGTAGCACGTGTCATGACCAAACTCCTACATCTCACTCACTCTCTCACCGATTCCAATCTCCTCACTCCTCGAGACCACTCTCTTTCCTTAGAAGGTCTAACCAAAATCGTCAACGGCGACGAAACATTCCACCTCAGCCTCGTTTGTGCTGAGCTCGCAGATAGTCTCGCCCACGCAGCTAACTCTGTTTCAAGACTGAGCAACCGCTGCACCACGGCCAGCCTCCGTTCTTTCCACCGCTTATTCCATGAATTTGCCGACATGGGTCGTGACCCTCATGGATGGGTCATGAACTGTAAAGACACAGAAGCTAAAAACAAGAAAATCGAAAGATACGTGAGCGTGACGACAGCTTTGTATAGAGAGATGGAAGAAATGGCGATATTAGAGAACTCTCTACGAAAACAAAGTTTACAGATTGGGATCGAATTCGAAGAAGAAGAAGACTACGAGAACAAGAAAGATGTTATGAAAGTGATAGATCTACAGAACAAGATTGAGAGACAGAAACAACATGTCAAGTATCTAAAAGACAGATCTCTCTGGAACAAAAGCTTCGACACTGTCGTGTTGATTCTCGCCAGATCGGTATTCACAGCACTCGCTAGACTCAAATCCGTCTTCTCCTCCGCCGCAGCCACTGGGTACATGGTTCCGACCGTCGTGTCTTCTCTCCCTCGTTCTCTCTCTTCTTCTTCTTCCTCTATGAATCTTGTTCACCCTAGCCCAAACGACGAAGAGAGAGACAAGACGACGACGTCTTCTGCGTTTCTTGAAGAAAGCTCAAGACTATTGAAACCACCGGAGACAACTCTCGGCGGAGCTGGCGTGGCGCTTCACTACGCGAATCTGATAGTAGTAATGGAGAAGATGATAAAGCAACCTCAGCTTGTAGGTCTTGACGCGAGAGACGATCTGTATTCAATGTTACCGGCAAGTGTGAGATCGTCACTTAGGTCAAGGCTAAAAGGAGTTGGTTTCACGGCGACGGACGGTGGTTTAGCGACGGAGTGGAAGGCGGCTTTAGGTCGGATCTTACGGTGGTTGTTACCATTGGCGCAGAATATGATTAGGTGGCAGAGTGAGAGAAGCTTTGAGCAGCAACACATGGCGACGGCAACGAATAGTCAGAACAGAGTGATGTTAGTTCAAACACTAGTGTTTGCAGATAAAGTTAAGACAGAAGCAGCCATAACAGAGCTTCTTGTTGGGTTGAATTACATCTGGAGATTCGAAAGAGAGATGACTGCGAAAGCTCTGTTTAATCTACAGTGTCCTCCTACTAATTACAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3475 DUF668

Protein Analysis

474

Amino Acids

53.17

Weight (kDa)

9.15

Isoelectric Point (pI)

54.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3475 PF11961 39 - 95 3.6e-19 Domain of unknown function (DUF3475)
DUF668 PF05003 314 - 401 6.4e-32 Protein of unknown function (DUF668)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014490)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G51670 AT5G51670
fragaria_vesca FvH4_7g28220
malus_domestica MD01G1186200.v1.1 MD07G1258400.v1.1
prunus_persica Prupe.2G282900_v2.0.a1
pyrus_communis pycom01g19780
rosa_chinensis RchiOBHm_Chr1g0375201
rosa_laevigata RLG00000026680
rosa_multiflora Rmu_sc0000554.1_g000026
rosa_roxburghii Rroxscaffold_4G00282950
rosa_rugosa Rorug01G0389000
rosa_samantha Rh1AG399700 Rh1BG364800 Rh1CG377700 Rh1DG395400
rosa_wichuraiana Rw1G035480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 793, 886
AatII GACGTC 1 cut(s) 890
AccI GTMKAC 1 cut(s) 64
AccII CGCG 2 cut(s) 974, 1034
AciI CCGC 6 cut(s) 334, 367, 759, 762, 948, 1148
AclWI GGATC 2 cut(s) 560, 1169
AcoI YGGCCR 1 cut(s) 345
AcsI RAATTY 2 cut(s) 380, 557
AcvI CACGTG 1 cut(s) 137
AcyI GRCGYC 1 cut(s) 887
AfaI GTAC 1 cut(s) 776
AflIII ACRYGT 2 cut(s) 136, 643
AgsI TTSAA 6 cut(s) 321, 905, 925, 1053, 1280, 1345
AhlI ACTAGT 1 cut(s) 1285
AleI CACNNNNGTG 1 cut(s) 695
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 6 cut(s) 195, 299, 625, 867, 929, 1032
AlwI GGATC 2 cut(s) 560, 1169
AlwNI CAGNNNCTG 1 cut(s) 770
Ama87I CYCGRG 1 cut(s) 198
AoxI GGCC 1 cut(s) 345
ApeKI GCWGC 5 cut(s) 305, 336, 764, 1228, 1316
ApoI RAATTY 2 cut(s) 380, 557
ArsI GACNNNNNNTTYG 2 cut(s) 466, 498
Asp700I GAANNNNTTC 1 cut(s) 15
AspLEI GCGC 2 cut(s) 964, 1189
AsuHPI GGTGA 2 cut(s) 166, 839
AsuII TTCGAA 3 cut(s) 126, 561, 1362
AvaI CYCGRG 1 cut(s) 198
BanII GRGCYC 1 cut(s) 285
BbrPI CACGTG 1 cut(s) 137
BbsI GAAGAC 4 cut(s) 579, 742, 789, 882
Bbv12I GWGCWC 1 cut(s) 285
BbvCI CCTCAGC 2 cut(s) 263, 1014
BbvI GCAGC 5 cut(s) 317, 323, 776, 1240, 1328
BccI CCATC 2 cut(s) 405, 490
BceAI ACGGC 4 cut(s) 259, 360, 1128, 1260
BcoDI GTCTC 6 cut(s) 195, 299, 625, 867, 929, 1032
BcuI ACTAGT 1 cut(s) 1285
BfaI CTAG 3 cut(s) 735, 852, 1286
BfmI CTRYAG 2 cut(s) 614, 1397
BfoI RGCGCY 1 cut(s) 965
BglII AGATCT 2 cut(s) 610, 665
BisI GCNGC 7 cut(s) 306, 337, 762, 765, 1149, 1229, 1317
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 7 cut(s) 307, 338, 763, 766, 1150, 1230, 1318
BmeT110I CYCGRG 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 783
BmrI ACTGGG 1 cut(s) 780
BmuI ACTGGG 1 cut(s) 780
BoxI GACNNNNGTC 1 cut(s) 393
BpiI GAAGAC 4 cut(s) 579, 742, 789, 882
BpmI CTGGAG 1 cut(s) 1375
Bpu10I CCTNAGC 2 cut(s) 263, 1014
Bpu1102I GCTNAGC 1 cut(s) 279
Bpu14I TTCGAA 3 cut(s) 126, 561, 1362
BpuEI CTTGAG 1 cut(s) 898
BsaAI YACGTR 2 cut(s) 137, 466
BsaBI GATNNNNATC 2 cut(s) 183, 551
BsaHI GRCGYC 1 cut(s) 887
BsaI GGTCTC 1 cut(s) 195
BsaJI CCNNGG 1 cut(s) 342
BsaWI WCCGGW 1 cut(s) 931
BsaXI ACNNNNNCTCC 2 cut(s) 942, 972
Bse118I RCCGGY 1 cut(s) 1060
Bse1I ACTGG 1 cut(s) 775
Bse8I GATNNNNATC 2 cut(s) 183, 551
BseDI CCNNGG 1 cut(s) 342
BseGI GGATG 1 cut(s) 416
BseJI GATNNNNATC 2 cut(s) 183, 551
BseMII CTCAG 4 cut(s) 270, 277, 317, 1028
BseNI ACTGG 1 cut(s) 775
BseRI GAGGAG 4 cut(s) 72, 179, 186, 745
BseXI GCAGC 5 cut(s) 317, 323, 776, 1240, 1328
BsgI GTGCAG 1 cut(s) 322
Bsh1236I CGCG 2 cut(s) 974, 1034
Bsh1285I CGRYCG 1 cut(s) 789
BshFI GGCC 1 cut(s) 347
BsiEI CGRYCG 1 cut(s) 789
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 198
BsiSI CCGG 2 cut(s) 932, 1061
BsmAI GTCTC 6 cut(s) 195, 299, 625, 867, 929, 1032
BsmBI CGTCTC 1 cut(s) 1032
BsnI GGCC 1 cut(s) 347
Bso31I GGTCTC 1 cut(s) 195
BsoBI CYCGRG 1 cut(s) 198
Bsp119I TTCGAA 3 cut(s) 126, 561, 1362
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 6 cut(s) 334, 367, 759, 762, 948, 1148
BspANI GGCC 1 cut(s) 347
BspCNI CTCAG 4 cut(s) 271, 276, 318, 1027
BspFNI CGCG 2 cut(s) 974, 1034
BspHI TCATGA 2 cut(s) 141, 417
BspLI GGNNCC 1 cut(s) 783
BspPI GGATC 2 cut(s) 560, 1169
BspT104I TTCGAA 3 cut(s) 126, 561, 1362
BspTNI GGTCTC 1 cut(s) 195
BsrFI RCCGGY 1 cut(s) 1060
BsrI ACTGG 1 cut(s) 775
BssAI RCCGGY 1 cut(s) 1060
BssECI CCNNGG 1 cut(s) 342
BssMI GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
BssNI GRCGYC 1 cut(s) 887
Bst4CI ACNGT 6 cut(s) 425, 694, 790, 1124, 1170, 1401
Bst6I CTCTTC 2 cut(s) 823, 861
BstACI GRCGYC 1 cut(s) 887
BstAPI GCANNNNNTGC 1 cut(s) 336
BstBAI YACGTR 2 cut(s) 137, 466
BstBI TTCGAA 3 cut(s) 126, 561, 1362
BstC8I GCNNGC 3 cut(s) 285, 349, 955
BstDEI CTNAG 6 cut(s) 217, 263, 279, 326, 1014, 1082
BstDSI CCRYGG 1 cut(s) 342
BstF5I GGATG 1 cut(s) 416
BstFNI CGCG 2 cut(s) 974, 1034
BstH2I RGCGCY 1 cut(s) 965
BstHHI GCGC 2 cut(s) 964, 1189
BstKTI GATC 8 cut(s) 77, 555, 613, 668, 716, 1045, 1076, 1164
BstMAI GTCTC 6 cut(s) 195, 299, 625, 867, 929, 1032
BstMBI GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
BstMCI CGRYCG 1 cut(s) 789
BstMWI GCNNNNNNNGC 6 cut(s) 284, 305, 336, 954, 959, 1015
BstNSI RCATGY 1 cut(s) 647
BstPAI GACNNNNGTC 1 cut(s) 393
BstSFI CTRYAG 2 cut(s) 614, 1397
BstUI CGCG 2 cut(s) 974, 1034
BstV1I GCAGC 5 cut(s) 317, 323, 776, 1240, 1328
BstV2I GAAGAC 4 cut(s) 579, 742, 789, 882
BstX2I RGATCY 3 cut(s) 610, 665, 1161
BstYI RGATCY 3 cut(s) 610, 665, 1161
BsuRI GGCC 1 cut(s) 347
BtgI CCRYGG 1 cut(s) 342
BtsCI GGATG 1 cut(s) 416
BtsIMutI CAGTG 3 cut(s) 690, 768, 1406
Cac8I GCNNGC 3 cut(s) 285, 349, 955
CaiI CAGNNNCTG 1 cut(s) 770
CciI TCATGA 2 cut(s) 141, 417
CfoI GCGC 2 cut(s) 964, 1189
Cfr10I RCCGGY 1 cut(s) 1060
CseI GACGC 1 cut(s) 1040
Csp6I GTAC 1 cut(s) 775
CviAII CATG 8 cut(s) 142, 377, 391, 407, 418, 644, 778, 1237
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 6 cut(s) 217, 263, 279, 326, 1014, 1082
DpnI GATC 8 cut(s) 76, 554, 612, 667, 715, 1044, 1075, 1163
DpnII GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
DrdI GACNNNNNNGTC 2 cut(s) 793, 886
DseDI GACNNNNNNGTC 2 cut(s) 793, 886
EaeI YGGCCR 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 823, 861
EarI CTCTTC 2 cut(s) 823, 861
EciI GGCGGA 2 cut(s) 748, 963
Ecl136II GAGCTC 1 cut(s) 283
Eco24I GRGCYC 1 cut(s) 285
Eco31I GGTCTC 1 cut(s) 195
Eco32I GATATC 1 cut(s) 90
Eco53kI GAGCTC 1 cut(s) 283
Eco72I CACGTG 1 cut(s) 137
Eco88I CYCGRG 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 283
EcoRI GAATTC 1 cut(s) 557
EcoRV GATATC 1 cut(s) 90
EcoT38I GRGCYC 1 cut(s) 285
Esp3I CGTCTC 1 cut(s) 1032
FaeI CATG 8 cut(s) 145, 380, 394, 410, 421, 647, 781, 1240
FatI CATG 8 cut(s) 141, 376, 390, 406, 417, 643, 777, 1236
FblI GTMKAC 1 cut(s) 64
Fnu4HI GCNGC 7 cut(s) 306, 337, 762, 765, 1149, 1229, 1317
FokI GGATG 1 cut(s) 423
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 7 cut(s) 306, 337, 762, 765, 1149, 1229, 1317
FspBI CTAG 3 cut(s) 735, 852, 1286
GlaI GCGC 2 cut(s) 963, 1188
GluI GCNGC 7 cut(s) 306, 337, 762, 765, 1149, 1229, 1317
GsuI CTGGAG 1 cut(s) 1375
HaeII RGCGCY 1 cut(s) 965
HaeIII GGCC 1 cut(s) 347
HapII CCGG 2 cut(s) 932, 1061
HgaI GACGC 1 cut(s) 1040
HhaI GCGC 2 cut(s) 964, 1189
Hin1I GRCGYC 1 cut(s) 887
Hin1II CATG 8 cut(s) 145, 380, 394, 410, 421, 647, 781, 1240
Hin6I GCGC 2 cut(s) 962, 1187
HinP1I GCGC 2 cut(s) 962, 1187
HincII GTYRAC 2 cut(s) 65, 241
HindII GTYRAC 2 cut(s) 65, 241
HindIII AAGCTT 2 cut(s) 682, 1219
HinfI GANTC 6 cut(s) 179, 703, 738, 838, 976, 1359
HpaII CCGG 2 cut(s) 932, 1061
HphI GGTGA 2 cut(s) 166, 839
Hpy166II GTNNAC 4 cut(s) 65, 241, 542, 847
Hpy188I TCNGA 6 cut(s) 87, 113, 786, 981, 1161, 1260
Hpy8I GTNNAC 4 cut(s) 65, 241, 542, 847
HpyAV CCTTC 2 cut(s) 215, 1138
HpyCH4III ACNGT 6 cut(s) 425, 694, 790, 1124, 1170, 1401
HpyCH4IV ACGT 3 cut(s) 136, 465, 887
HpyCH4V TGCA 2 cut(s) 339, 1295
HpyF10VI GCNNNNNNNGC 6 cut(s) 284, 305, 336, 954, 959, 1015
HpyF3I CTNAG 6 cut(s) 217, 263, 279, 326, 1014, 1082
HpySE526I ACGT 3 cut(s) 136, 465, 887
Hsp92I GRCGYC 1 cut(s) 887
Hsp92II CATG 8 cut(s) 145, 380, 394, 410, 421, 647, 781, 1240
HspAI GCGC 2 cut(s) 962, 1187
Kzo9I GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
LmnI GCTCC 1 cut(s) 950
LpnPI CCDG 8 cut(s) 361, 658, 724, 756, 939, 945, 1074, 1339
Lsp1109I GCAGC 5 cut(s) 317, 323, 776, 1240, 1328
MaeI CTAG 3 cut(s) 735, 852, 1286
MaeII ACGT 3 cut(s) 136, 465, 887
MaeIII GTNAC 5 cut(s) 398, 472, 1056, 1077, 1176
MalI GATC 8 cut(s) 76, 554, 612, 667, 715, 1044, 1075, 1163
MboI GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
MflI RGATCY 3 cut(s) 610, 665, 1161
MhlI GDGCHC 1 cut(s) 285
MluCI AATT 4 cut(s) 380, 557, 1345, 1414
MlyI GAGTC 1 cut(s) 732
MmeI TCCRAC 3 cut(s) 91, 809, 1139
MroXI GAANNNNTTC 1 cut(s) 15
MseI TTAA 2 cut(s) 1305, 1392
MslI CAYNNNNRTG 1 cut(s) 695
MspA1I CMGCKG 1 cut(s) 336
MspI CCGG 2 cut(s) 932, 1061
MvnI CGCG 2 cut(s) 974, 1034
MwoI GCNNNNNNNGC 6 cut(s) 284, 305, 336, 954, 959, 1015
NdeII GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
NlaIII CATG 8 cut(s) 145, 380, 394, 410, 421, 647, 781, 1240
NlaIV GGNNCC 1 cut(s) 783
NmeAIII GCCGAG 1 cut(s) 924
NmuCI GTSAC 3 cut(s) 398, 472, 1077
NspI RCATGY 1 cut(s) 647
NspV TTCGAA 3 cut(s) 126, 561, 1362
OliI CACNNNNGTG 1 cut(s) 695
PaeR7I CTCGAG 1 cut(s) 198
PagI TCATGA 2 cut(s) 141, 417
PciI ACATGT 1 cut(s) 643
PcsI WCGNNNNNNNCGW 6 cut(s) 70, 243, 462, 693, 893, 1038
PdmI GAANNNNTTC 1 cut(s) 15
PfeI GAWTC 5 cut(s) 179, 703, 838, 976, 1359
PflFI GACNNNGTC 2 cut(s) 692, 886
PkrI GCNGC 7 cut(s) 307, 338, 763, 766, 1150, 1230, 1318
PleI GAGTC 1 cut(s) 732
PmaCI CACGTG 1 cut(s) 137
PmlI CACGTG 1 cut(s) 137
PpsI GAGTC 1 cut(s) 732
Ppu21I YACGTR 2 cut(s) 137, 466
PscI ACATGT 1 cut(s) 643
PshAI GACNNNNGTC 1 cut(s) 393
Psp124BI GAGCTC 1 cut(s) 285
PspCI CACGTG 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 783
PstNI CAGNNNCTG 1 cut(s) 770
PsuI RGATCY 3 cut(s) 610, 665, 1161
PsyI GACNNNGTC 2 cut(s) 692, 886
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
RseI CAYNNNNRTG 1 cut(s) 695
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 63
SaqAI TTAA 2 cut(s) 1305, 1392
SatI GCNGC 7 cut(s) 306, 337, 762, 765, 1149, 1229, 1317
Sau3AI GATC 8 cut(s) 74, 552, 610, 665, 713, 1042, 1073, 1161
SchI GAGTC 1 cut(s) 732
SduI GDGCHC 1 cut(s) 285
SfcI CTRYAG 2 cut(s) 614, 1397
Sfr274I CTCGAG 1 cut(s) 198
SfuI TTCGAA 3 cut(s) 126, 561, 1362
SlaI CTCGAG 1 cut(s) 198
SmiMI CAYNNNNRTG 1 cut(s) 695
SmlI CTYRAG 2 cut(s) 198, 913
SmoI CTYRAG 2 cut(s) 198, 913
SpeI ACTAGT 1 cut(s) 1285
Sse9I AATT 4 cut(s) 380, 557, 1345, 1414
SsiI CCGC 6 cut(s) 334, 367, 759, 762, 948, 1148
SspMI CTAG 3 cut(s) 735, 852, 1286
SstI GAGCTC 1 cut(s) 285
TaaI ACNGT 6 cut(s) 425, 694, 790, 1124, 1170, 1401
TaiI ACGT 3 cut(s) 139, 468, 890
TaqI TCGA 9 cut(s) 9, 64, 126, 199, 456, 555, 561, 687, 1362
TasI AATT 4 cut(s) 380, 557, 1345, 1414
TauI GCSGC 2 cut(s) 764, 1151
TfiI GAWTC 5 cut(s) 179, 703, 838, 976, 1359
Tru1I TTAA 2 cut(s) 1305, 1392
Tru9I TTAA 2 cut(s) 1305, 1392
TscAI CASTG 3 cut(s) 697, 775, 1406
TseFI GTSAC 3 cut(s) 398, 472, 1077
TseI GCWGC 5 cut(s) 305, 336, 764, 1228, 1316
Tsp45I GTSAC 3 cut(s) 398, 472, 1077
TspDTI ATGAA 4 cut(s) 393, 434, 614, 851
TspGWI ACGGA 5 cut(s) 97, 344, 736, 1133, 1151
TspRI CASTG 3 cut(s) 697, 775, 1406
Tth111I GACNNNGTC 2 cut(s) 692, 886
XapI RAATTY 2 cut(s) 380, 557
XceI RCATGY 1 cut(s) 647
XcmI CCANNNNNNNNNTGG 1 cut(s) 775
XhoI CTCGAG 1 cut(s) 198
XmiI GTMKAC 1 cut(s) 64
XmnI GAANNNNTTC 1 cut(s) 15
XspI CTAG 3 cut(s) 735, 852, 1286
ZraI GACGTC 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.