AT5G51710

efflux antiporter 5

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0007412
Physical Location & Seq
Reverse (-)
21004251 .. 21008849
4599 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G51710.2

Sequence Viewer

Length: 1707 bp
ATGGCGAGATTCGCAGTGATTGGATTAACGTTCCTCCTTTTACTTGGTACGTCCTTATCAGCGAGATCTGATGAAGAAACACGCGAGCGATTTTACGGCAACGTCGTGAACTCGACGGCTCCGGGAAACGGTGAAGGAAGTATCGCTAAAATGTTCGATCGAGTTCTGGAGAAAGAATTTTCCGAAAACGATTCGCCTGAAGGATCTGATGGAGCTAGCTTTAACAGCAGTGTTGCTGATCAACAAGCTGAAATAGAGACTGTTGCTAAAGTAACTCATGAGAAGGGCAAAAGAAATGATACACAAGAAAATAATGGTACGAGACCGTTTCAACTTCAAGATGTGTTTTCCCTGGAGAATGAAGATTCTGATGATATGACTCTTATTGACAAGAAGAATAATGTTTTTGTCATGTCAAACAAGAAATCTAAGTATCCGATACTTCAAGTAGATTTGAGATTAATATCAGATTTGGTGGTCATTATTGTTTTTGCTGCAATTGGTGGCATCGTCTTCTCTTGTCTTGGACAACCGGTCATTGTTGGATATCTTCTTGCGGGGTCAATCATTGGGCCAGGAGGATTGAAATTCATTAGTGAAATGGTTCAGGTTGAAACCGTAGCACAGTTTGGTGTTGTTTTCCTTCTGTTTGCTTTGGGTTTGGAATTCTCAATGACTAAGCTGAAAGTCGTTGGCCCAGTTGCTGTCCTTGGAGGGCTTCTTCAAATTGTCTTACTCATGTTTTTGTGTGGTGTAACTGCCTTGTTGTGTGGAGCAAGGTTGTCAGAGGGTATTTTTGTTGGTGCCTTTTTATCTATGTCATCAACTGCAGTGGTGGTAAAGTTTTTGGTAGAACGGAACAGTACAAGTTCTCTTCATGGTCAAGTTACTATAGGGATACTTATCTTTCAGGACTGTGTTGTTGGTTTACTATTCGCCTTGCTTCCAGTTTTGGGTGGTAACAGTGGTTTACTACAAGGAATTATATCAATGGGAAAGCTGCTACTGATATTGTCCATATATCTCACTGTGGCATCTCTATTAACGTGGTCATTTGTACCTCGTTTTCTGAAGCTAATGATTCAATTATCATCTCAAACAAATGAACTTTACCAGTTAGCTGCTGTGGCATTCTGCTTACTATCTGCATGGTGCAGTGACAAGCTGGGTCTTAGTCTTGAGTTGGGATCATTTGTGGCTGGCGTTATGTTGTCTACGACTGAATTTGCTCAACATACACTAGAGCAGGTGGAACCAATTCGTAATCTGTTTGCCGCTCTCTTTCTATCTAGTATCGGGATGCTCATTAATGTTCACTTTCTATGGAACCACGTGGATATCCTTCTAGCGTCGGTGATTCTAGTGATAGTTATTAAGACAGCAATTGCAGCAGTAGTTGTTAAGGCTTTTAGGTACAACATGAGGATATCTTTCCATGTCGGGGTACTGCTTGCTCAGATTGGAGAGTTCGCCTTTGTTCTTCTAAGCCGAGCGTCAAACCTACACGTAATCGAGGGGAAAATGTATCTACTCCTTTTGGGCACAACTGCTCTAAGTCTCGTAACGACTCCGTTGCTATTCAAGTTAATACCGAGTGCAATGAACCTCGGTGTCCTGCTTCGATGGTTCCCTTCTGAGAACAGTTCTCCGAACGAGAGTTTGCAGGAGAAAGCATCATTGATAGAAGTACATAACAGAACCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

568

Amino Acids

61.6

Weight (kDa)

5.84

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 162 - 528 6.8e-78 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1237
Acc36I ACCTGC 1 cut(s) 1237
AccB1I GGYRCC 1 cut(s) 803
AccBSI CCGCTC 1 cut(s) 1277
AccI GTMKAC 1 cut(s) 1214
AccII CGCG 1 cut(s) 84
AciI CCGC 2 cut(s) 557, 1275
AclI AACGTT 1 cut(s) 29
AclWI GGATC 2 cut(s) 211, 1195
AcsI RAATTY 4 cut(s) 176, 587, 665, 1223
AcuI CTGAAG 2 cut(s) 219, 1091
AcvI CACGTG 1 cut(s) 1333
AfaI GTAC 7 cut(s) 49, 319, 865, 1059, 1415, 1446, 1689
AfiI CCNNNNNNNGG 3 cut(s) 128, 953, 1441
AflIII ACRYGT 1 cut(s) 1504
AgeI ACCGGT 1 cut(s) 532
AgsI TTSAA 8 cut(s) 332, 338, 446, 586, 614, 725, 1085, 1582
AhdI GACNNNNNGTC 1 cut(s) 533
AjnI CCWGG 2 cut(s) 351, 574
AluBI AGCT 8 cut(s) 215, 219, 248, 682, 1000, 1075, 1121, 1165
AluI AGCT 8 cut(s) 215, 219, 248, 682, 1000, 1075, 1121, 1165
Alw26I GTCTC 3 cut(s) 251, 316, 1562
AlwI GGATC 2 cut(s) 211, 1195
AlwNI CAGNNNCTG 1 cut(s) 704
AoxI GGCC 2 cut(s) 572, 694
ApeKI GCWGC 4 cut(s) 494, 1000, 1121, 1388
ApoI RAATTY 4 cut(s) 176, 587, 665, 1223
AseI ATTAAT 2 cut(s) 461, 1308
AsiGI ACCGGT 1 cut(s) 532
Asp700I GAANNNNTTC 2 cut(s) 603, 1257
AspS9I GGNCC 2 cut(s) 572, 695
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 143, 1366
AsuNHI GCTAGC 1 cut(s) 215
BaeGI GKGCMC 1 cut(s) 1544
BanI GGYRCC 1 cut(s) 803
BbrPI CACGTG 1 cut(s) 1333
BbsI GAAGAC 1 cut(s) 505
BbvI GCAGC 4 cut(s) 481, 987, 1108, 1400
BccI CCATC 2 cut(s) 203, 1617
BceAI ACGGC 2 cut(s) 112, 132
BcgI CGANNNNNNTGC 2 cut(s) 1555, 1589
BciT130I CCWGG 2 cut(s) 353, 576
BciVI GTATCC 2 cut(s) 444, 891
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 3 cut(s) 251, 316, 1562
BfaI CTAG 5 cut(s) 216, 1241, 1290, 1346, 1361
BfmI CTRYAG 2 cut(s) 828, 891
BfuAI ACCTGC 1 cut(s) 1237
BfuI GTATCC 2 cut(s) 444, 891
BglII AGATCT 1 cut(s) 65
BisI GCNGC 5 cut(s) 495, 1001, 1122, 1275, 1389
BlsI GCNGC 5 cut(s) 496, 1002, 1123, 1276, 1390
Bme1390I CCNGG 3 cut(s) 123, 353, 576
BmeRI GACNNNNNGTC 1 cut(s) 533
BmgT120I GGNCC 2 cut(s) 572, 695
BmiI GGNNCC 5 cut(s) 120, 805, 1254, 1328, 1628
BmrFI CCNGG 3 cut(s) 123, 353, 576
BmrI ACTGGG 1 cut(s) 692
BmsI GCATC 4 cut(s) 516, 1043, 1290, 1682
BmtI GCTAGC 1 cut(s) 219
BmuI ACTGGG 1 cut(s) 692
BpiI GAAGAC 1 cut(s) 505
BpmI CTGGAG 2 cut(s) 188, 374
BpuEI CTTGAG 1 cut(s) 1199
BpuMI CCSGG 1 cut(s) 123
BsaAI YACGTR 2 cut(s) 1333, 1507
BsaBI GATNNNNATC 2 cut(s) 463, 902
BsaI GGTCTC 1 cut(s) 316
BsaJI CCNNGG 3 cut(s) 351, 709, 1606
BsaWI WCCGGW 1 cut(s) 532
Bsc4I CCNNNNNNNGG 3 cut(s) 128, 953, 1441
Bse118I RCCGGY 1 cut(s) 532
Bse1I ACTGG 3 cut(s) 698, 947, 1114
Bse3DI GCAATG 1 cut(s) 1605
Bse8I GATNNNNATC 2 cut(s) 463, 902
BseBI CCWGG 2 cut(s) 353, 576
BseDI CCNNGG 3 cut(s) 351, 709, 1606
BseGI GGATG 1 cut(s) 1305
BseJI GATNNNNATC 2 cut(s) 463, 902
BseLI CCNNNNNNNGG 3 cut(s) 128, 953, 1441
BseMI GCAATG 1 cut(s) 1605
BseMII CTCAG 2 cut(s) 1469, 1626
BseNI ACTGG 3 cut(s) 698, 947, 1114
BseSI GKGCMC 1 cut(s) 1544
BseXI GCAGC 4 cut(s) 481, 987, 1108, 1400
BseYI CCCAGC 1 cut(s) 1165
BsgI GTGCAG 1 cut(s) 1174
Bsh1236I CGCG 1 cut(s) 84
Bsh1285I CGRYCG 1 cut(s) 160
BshFI GGCC 2 cut(s) 574, 696
BshNI GGYRCC 1 cut(s) 803
BshTI ACCGGT 1 cut(s) 532
BsiEI CGRYCG 1 cut(s) 160
BsiSI CCGG 2 cut(s) 122, 533
BslI CCNNNNNNNGG 3 cut(s) 128, 953, 1441
BsmAI GTCTC 3 cut(s) 251, 316, 1562
BsmI GAATGC 1 cut(s) 1130
BsnI GGCC 2 cut(s) 574, 696
Bso31I GGTCTC 1 cut(s) 316
Bsp1286I GDGCHC 1 cut(s) 1544
Bsp143I GATC 5 cut(s) 65, 157, 203, 238, 1187
BspACI CCGC 2 cut(s) 557, 1275
BspANI GGCC 2 cut(s) 574, 696
BspCNI CTCAG 2 cut(s) 1468, 1627
BspFNI CGCG 1 cut(s) 84
BspHI TCATGA 1 cut(s) 277
BspLI GGNNCC 5 cut(s) 120, 805, 1254, 1328, 1628
BspMAI CTGCAG 1 cut(s) 832
BspMI ACCTGC 1 cut(s) 1237
BspOI GCTAGC 1 cut(s) 219
BspPI GGATC 2 cut(s) 211, 1195
BspT107I GGYRCC 1 cut(s) 803
BspTNI GGTCTC 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 1277
BsrDI GCAATG 1 cut(s) 1605
BsrFI RCCGGY 1 cut(s) 532
BsrI ACTGG 3 cut(s) 698, 947, 1114
BssAI RCCGGY 1 cut(s) 532
BssECI CCNNGG 3 cut(s) 351, 709, 1606
BssMI GATC 5 cut(s) 65, 157, 203, 238, 1187
BssT1I CCWWGG 1 cut(s) 709
Bst2UI CCWGG 2 cut(s) 353, 576
Bst6I CTCTTC 1 cut(s) 879
BstBAI YACGTR 2 cut(s) 1333, 1507
BstC8I GCNNGC 4 cut(s) 86, 217, 1201, 1452
BstDEI CTNAG 7 cut(s) 429, 678, 1172, 1455, 1484, 1553, 1635
BstF5I GGATG 1 cut(s) 1305
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 5 cut(s) 68, 160, 206, 241, 1190
BstMAI GTCTC 3 cut(s) 251, 316, 1562
BstMBI GATC 5 cut(s) 65, 157, 203, 238, 1187
BstMCI CGRYCG 1 cut(s) 160
BstMWI GCNNNNNNNGC 4 cut(s) 11, 225, 1127, 1388
BstNI CCWGG 2 cut(s) 353, 576
BstSCI CCNGG 3 cut(s) 121, 351, 574
BstSFI CTRYAG 2 cut(s) 828, 891
BstSLI GKGCMC 1 cut(s) 1544
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 4 cut(s) 481, 987, 1108, 1400
BstV2I GAAGAC 1 cut(s) 505
BstX2I RGATCY 2 cut(s) 65, 203
BstYI RGATCY 2 cut(s) 65, 203
BsuI GTATCC 2 cut(s) 444, 891
BsuRI GGCC 2 cut(s) 574, 696
BtsCI GGATG 1 cut(s) 1305
BtsI GCAGTG 4 cut(s) 21, 235, 837, 1162
BtsIMutI CAGTG 6 cut(s) 21, 235, 837, 970, 1026, 1162
BveI ACCTGC 1 cut(s) 1237
Cac8I GCNNGC 4 cut(s) 86, 217, 1201, 1452
CaiI CAGNNNCTG 1 cut(s) 704
CciI TCATGA 1 cut(s) 277
Cfr10I RCCGGY 1 cut(s) 532
Cfr13I GGNCC 2 cut(s) 572, 695
CseI GACGC 2 cut(s) 1338, 1482
Csp6I GTAC 7 cut(s) 48, 318, 864, 1058, 1414, 1445, 1688
CspAI ACCGGT 1 cut(s) 532
CspCI CAANNNNNGTGG 2 cut(s) 813, 848
CviAII CATG 7 cut(s) 278, 412, 739, 878, 1149, 1420, 1436
CviQI GTAC 7 cut(s) 48, 318, 864, 1058, 1414, 1445, 1688
DdeI CTNAG 7 cut(s) 429, 678, 1172, 1455, 1484, 1553, 1635
DpnI GATC 5 cut(s) 67, 159, 205, 240, 1189
DpnII GATC 5 cut(s) 65, 157, 203, 238, 1187
DriI GACNNNNNGTC 1 cut(s) 533
Eam1104I CTCTTC 1 cut(s) 879
Eam1105I GACNNNNNGTC 1 cut(s) 533
EarI CTCTTC 1 cut(s) 879
Eco130I CCWWGG 1 cut(s) 709
Eco31I GGTCTC 1 cut(s) 316
Eco32I GATATC 3 cut(s) 548, 1339, 1428
Eco57I CTGAAG 2 cut(s) 219, 1091
Eco72I CACGTG 1 cut(s) 1333
EcoRI GAATTC 1 cut(s) 665
EcoRII CCWGG 2 cut(s) 351, 574
EcoRV GATATC 3 cut(s) 548, 1339, 1428
EcoT14I CCWWGG 1 cut(s) 709
ErhI CCWWGG 1 cut(s) 709
FaeI CATG 7 cut(s) 281, 415, 742, 881, 1152, 1423, 1439
FatI CATG 7 cut(s) 277, 411, 738, 877, 1148, 1419, 1435
FauI CCCGC 1 cut(s) 550
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 1 cut(s) 1214
Fnu4HI GCNGC 5 cut(s) 495, 1001, 1122, 1275, 1389
FokI GGATG 1 cut(s) 1312
Fsp4HI GCNGC 5 cut(s) 495, 1001, 1122, 1275, 1389
FspBI CTAG 5 cut(s) 216, 1241, 1290, 1346, 1361
GluI GCNGC 5 cut(s) 495, 1001, 1122, 1275, 1389
GsaI CCCAGC 1 cut(s) 1169
GsuI CTGGAG 2 cut(s) 188, 374
HaeIII GGCC 2 cut(s) 574, 696
HapII CCGG 2 cut(s) 122, 533
HgaI GACGC 2 cut(s) 1338, 1482
Hin1II CATG 7 cut(s) 281, 415, 742, 881, 1152, 1423, 1439
HinfI GANTC 7 cut(s) 9, 191, 365, 379, 1081, 1357, 1567
HpaII CCGG 2 cut(s) 122, 533
HphI GGTGA 2 cut(s) 143, 1366
Hpy166II GTNNAC 5 cut(s) 109, 929, 971, 1215, 1315
Hpy188III TCNNGA 7 cut(s) 106, 167, 278, 338, 911, 1178, 1297
Hpy8I GTNNAC 5 cut(s) 109, 929, 971, 1215, 1315
Hpy99I CGWCG 3 cut(s) 107, 118, 1354
HpyAV CCTTC 6 cut(s) 128, 194, 277, 653, 1352, 1641
HpyCH4IV ACGT 6 cut(s) 29, 50, 102, 1046, 1332, 1506
HpyCH4V TGCA 7 cut(s) 497, 830, 1148, 1155, 1388, 1598, 1663
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 225, 1127, 1388
HpyF3I CTNAG 7 cut(s) 429, 678, 1172, 1455, 1484, 1553, 1635
HpySE526I ACGT 6 cut(s) 29, 50, 102, 1046, 1332, 1506
Hsp92II CATG 7 cut(s) 281, 415, 742, 881, 1152, 1423, 1439
Ksp22I TGATCA 1 cut(s) 238
Kzo9I GATC 5 cut(s) 65, 157, 203, 238, 1187
LmnI GCTCC 3 cut(s) 124, 212, 773
Lsp1109I GCAGC 4 cut(s) 481, 987, 1108, 1400
LweI GCATC 4 cut(s) 516, 1043, 1290, 1682
MaeI CTAG 5 cut(s) 216, 1241, 1290, 1346, 1361
MaeII ACGT 6 cut(s) 29, 50, 102, 1046, 1332, 1506
MaeIII GTNAC 6 cut(s) 271, 754, 886, 959, 1157, 1561
MalI GATC 5 cut(s) 67, 159, 205, 240, 1189
MbiI CCGCTC 1 cut(s) 1277
MboI GATC 5 cut(s) 65, 157, 203, 238, 1187
MboII GAAGA 8 cut(s) 86, 374, 406, 505, 542, 713, 866, 1472
MfeI CAATTG 2 cut(s) 498, 1383
MflI RGATCY 2 cut(s) 65, 203
MhlI GDGCHC 1 cut(s) 1544
MlyI GAGTC 2 cut(s) 373, 1561
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 8 cut(s) 44, 572, 707, 781, 1071, 1416, 1507, 1616
MroXI GAANNNNTTC 2 cut(s) 603, 1257
MseI TTAA 8 cut(s) 26, 222, 461, 1043, 1308, 1374, 1401, 1586
MspI CCGG 2 cut(s) 122, 533
MspR9I CCNGG 3 cut(s) 123, 353, 576
MunI CAATTG 2 cut(s) 498, 1383
Mva1269I GAATGC 1 cut(s) 1130
MvaI CCWGG 2 cut(s) 353, 576
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 4 cut(s) 11, 225, 1127, 1388
NciI CCSGG 1 cut(s) 123
NdeII GATC 5 cut(s) 65, 157, 203, 238, 1187
NheI GCTAGC 1 cut(s) 215
NlaIII CATG 7 cut(s) 281, 415, 742, 881, 1152, 1423, 1439
NlaIV GGNNCC 5 cut(s) 120, 805, 1254, 1328, 1628
NmeAIII GCCGAG 1 cut(s) 1514
NmuCI GTSAC 1 cut(s) 1157
PagI TCATGA 1 cut(s) 277
PaqCI CACCTGC 1 cut(s) 1237
PcsI WCGNNNNNNNCGW 1 cut(s) 102
PctI GAATGC 1 cut(s) 1130
PdmI GAANNNNTTC 2 cut(s) 603, 1257
PfeI GAWTC 5 cut(s) 9, 191, 365, 1081, 1357
PfoI TCCNGGA 1 cut(s) 121
PinAI ACCGGT 1 cut(s) 532
PkrI GCNGC 5 cut(s) 496, 1002, 1123, 1276, 1390
Ple19I CGATCG 1 cut(s) 160
PleI GAGTC 2 cut(s) 373, 1561
PmaCI CACGTG 1 cut(s) 1333
PmlI CACGTG 1 cut(s) 1333
PpsI GAGTC 2 cut(s) 373, 1561
Ppu21I YACGTR 2 cut(s) 1333, 1507
PshBI ATTAAT 2 cut(s) 461, 1308
Psp1406I AACGTT 1 cut(s) 29
Psp6I CCWGG 2 cut(s) 351, 574
PspCI CACGTG 1 cut(s) 1333
PspFI CCCAGC 1 cut(s) 1165
PspGI CCWGG 2 cut(s) 351, 574
PspN4I GGNNCC 5 cut(s) 120, 805, 1254, 1328, 1628
PspPI GGNCC 2 cut(s) 572, 695
PstI CTGCAG 1 cut(s) 832
PstNI CAGNNNCTG 1 cut(s) 704
PsuI RGATCY 2 cut(s) 65, 203
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 7 cut(s) 49, 319, 865, 1059, 1415, 1446, 1689
RsaNI GTAC 7 cut(s) 48, 318, 864, 1058, 1414, 1445, 1688
SaqAI TTAA 8 cut(s) 26, 222, 461, 1043, 1308, 1374, 1401, 1586
SatI GCNGC 5 cut(s) 495, 1001, 1122, 1275, 1389
Sau3AI GATC 5 cut(s) 65, 157, 203, 238, 1187
Sau96I GGNCC 2 cut(s) 572, 695
SchI GAGTC 2 cut(s) 373, 1561
ScrFI CCNGG 3 cut(s) 123, 353, 576
SduI GDGCHC 1 cut(s) 1544
SfaNI GCATC 4 cut(s) 516, 1043, 1290, 1682
SfcI CTRYAG 2 cut(s) 828, 891
SmlI CTYRAG 1 cut(s) 1178
SmoI CTYRAG 1 cut(s) 1178
SsiI CCGC 2 cut(s) 557, 1275
SspMI CTAG 5 cut(s) 216, 1241, 1290, 1346, 1361
StyD4I CCNGG 3 cut(s) 121, 351, 574
StyI CCWWGG 1 cut(s) 709
TaiI ACGT 6 cut(s) 32, 53, 105, 1049, 1335, 1509
TaqI TCGA 5 cut(s) 113, 156, 160, 1512, 1621
TatI WGTACW 2 cut(s) 863, 1687
TauI GCSGC 1 cut(s) 1277
TfiI GAWTC 5 cut(s) 9, 191, 365, 1081, 1357
Tru1I TTAA 8 cut(s) 26, 222, 461, 1043, 1308, 1374, 1401, 1586
Tru9I TTAA 8 cut(s) 26, 222, 461, 1043, 1308, 1374, 1401, 1586
TscAI CASTG 6 cut(s) 21, 235, 837, 970, 1033, 1162
TseFI GTSAC 1 cut(s) 1157
TseI GCWGC 4 cut(s) 494, 1000, 1121, 1388
Tsp45I GTSAC 1 cut(s) 1157
TspDTI ATGAA 6 cut(s) 87, 375, 580, 866, 1119, 1616
TspGWI ACGGA 2 cut(s) 871, 1560
TspRI CASTG 6 cut(s) 21, 235, 837, 970, 1033, 1162
VspI ATTAAT 2 cut(s) 461, 1308
XapI RAATTY 4 cut(s) 176, 587, 665, 1223
XmiI GTMKAC 1 cut(s) 1214
XmnI GAANNNNTTC 2 cut(s) 603, 1257
XspI CTAG 5 cut(s) 216, 1241, 1290, 1346, 1361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.