MD01G1187400.v1.1

) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0007412
Physical Location & Seq
Reverse (-)
28597643 .. 28603274
5632 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1187400.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGCCAAGAGAAAACGATGCGTGTTTGGATTCTGCTATTGTCTTTTGACGATTTTGATTTGCGCTAGGGTTTCGGTTTCCGCGAGATCTGATAAGGAAATTCGGGAGAGGTTCTACGGCAACATGACGAACTCCTCCGCGCCGGATTCCGGCGACGGCACCATTGCCAAAATGTTCGATCGCGTCCTCGAGAAGGAGTTCTCCGAGAACGACCAGCCCGAAGGATCTGACGGGGCCAGCTTTAACAGCAGTGTGGCCGATCAACAAGCTGTACTAGAGACTGTGGCGAAAATCACTCACGAGAAGGGCAAGAAAAATGATACACACGTTGGAAACGGCACAAAACCATTTGATTTTGAAGGTGTTTTTTCCCTGGAGAATGAAGATTCTGAAGAAACAACAACCTTGATTGACAAAAAGGATAATGTGTTTGTGATGTCAAACAAGAAATCCAAATATCCAGTACTTCAAGTAGATTTAAGATTAATTTCTGATCTGGTGGTTATCATAGTGTCTGCTGCCATTGGTGGAATTCTGTTTTCTTGTTTGGGACAACCGGTTATTGTAGGATACCTGCTTGCCGGTTCTCTCATTGGACCAGGGGGTCTGACGTTCGTCAGTGAAATGGTGCAGGTTGAAACTGTTGCACAGTTTGGTGTTATATTTCTTCTTTTTGCTCTAGGTTTGGAGTTTTCTTTGGCTAAGTTAAAAGCCGTGGGCCCTGTAGCTGTTCTTGGAGGTCTTCTTCAAATCATGACATTTATGTTCCTGTGTGCCATAACTGCTATTTTATGTGGAGCAAAGTTATCTGAGGGAATTTTTGTTGGTTCCTTCCTGTCAATGTCATCAACAGCAGTGGTGGTGAAATTTCTTGCAGAAAAGAATAGTAGTAGTGCTCTTCATGGTCAAGTAACAATTGGAACACTCATTTTCCAGGATTGTGCTGTTGGTTTACTGTTTGCTTTGCTCCCAGTTTTGGGTGGTCATAGCGGTCTTTTCCAAGGAATGGTATCAGTGGGGAAGCTGTTGCTGGTGTTGTCACTATATCTTGGTGCTGCCTCAGTATTGTGTTGGTCATTTGTTCCTCGCTTTCTGAAACTGATGATGCATTTGTCCTCTCAAACAAATGAGCTTTATCAGCTTGCTGTTGTGGCTTTCTGCTTATTATCTGCTTGGTGTAGTGATAAGTTGGGCCTTAGCCTTGAGTTGGGTTCATTTCTGGCTGGGGTTATGATATCAACCACTGACCTTGCACAACATACTTTAGACCAGGTGGAACCAATTCGTAACCTATTTGCTGCTCTCTTCCTTTCGAGTATTGGAATGCTCATACATGTTCATTTCTTGTGGAGCCACGTCGACATACTGCTAGCATCTGTTATTCTAGTTATAGTTGTAAAGACTGCCGTTGCTACTATTGTTACCAAGGCCTTTGGATATAGCTTTAGGACATCATTTGTGGTTGGAGTCTCACTTGCTCAAATTGGAGAATTTGCTTTTGTTCTCTTAAGTCGAGCCTCGAACCTTAATCTTGTTGAGGGGAAAATGTACCTGCTTCTTCTCGGAACAACAGCGCTTAGCCTGGTTACAACTCCTCTCATGTTTAAGCTGACTCCTGCCATAATGAATCTAGGAGTTCTTATGCGCTGGTTTCCTTCTGAGGGAACTCCGTACAACGAGGAGAAAGCTGCGATGATTGACGCACAGCACAACCGAATACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

575

Amino Acids

61.94

Weight (kDa)

6.23

Isoelectric Point (pI)

28.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 170 - 536 1.6e-73 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 603
Acc36I ACCTGC 3 cut(s) 582, 622, 1560
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 1006
AccI GTMKAC 1 cut(s) 1359
AccII CGCG 3 cut(s) 83, 140, 183
AciI CCGC 3 cut(s) 81, 138, 990
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 2 cut(s) 3, 255
AcsI RAATTY 5 cut(s) 99, 531, 816, 866, 1490
AcuI CTGAAG 1 cut(s) 411
AfaI GTAC 4 cut(s) 273, 465, 1550, 1673
AfeI AGCGCT 1 cut(s) 1575
AfiI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
AflII CTTAAG 1 cut(s) 1507
AflIII ACRYGT 2 cut(s) 325, 1333
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 4 cut(s) 359, 470, 638, 749
AjiI CACGTC 1 cut(s) 1357
AjnI CCWGG 5 cut(s) 372, 598, 933, 1269, 1581
AluBI AGCT 9 cut(s) 240, 269, 728, 1024, 1132, 1141, 1443, 1609, 1688
AluI AGCT 9 cut(s) 240, 269, 728, 1024, 1132, 1141, 1443, 1609, 1688
Alw21I GWGCWC 1 cut(s) 898
Alw26I GTCTC 2 cut(s) 272, 1474
AlwI GGATC 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 1575
AoxI GGCC 6 cut(s) 3, 234, 255, 718, 1192, 1428
ApaI GGGCCC 1 cut(s) 722
ApeKI GCWGC 4 cut(s) 518, 1055, 1298, 1688
ApoI RAATTY 5 cut(s) 99, 531, 816, 866, 1490
AseI ATTAAT 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 556
Asp700I GAANNNNTTC 2 cut(s) 197, 1281
AspLEI GCGC 4 cut(s) 65, 142, 1576, 1647
AspS9I GGNCC 5 cut(s) 234, 596, 718, 719, 1192
AsuHPI GGTGA 1 cut(s) 874
AsuNHI GCTAGC 1 cut(s) 1369
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 596
BaeGI GKGCMC 1 cut(s) 722
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 1 cut(s) 722
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 734
Bbv12I GWGCWC 1 cut(s) 898
BbvI GCAGC 4 cut(s) 505, 1042, 1285, 1675
BceAI ACGGC 5 cut(s) 133, 172, 352, 698, 1391
BciT130I CCWGG 5 cut(s) 374, 600, 935, 1271, 1583
BciVI GTATCC 1 cut(s) 563
BcoDI GTCTC 2 cut(s) 272, 1474
BfaI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
BfmI CTRYAG 1 cut(s) 723
BfoI RGCGCY 1 cut(s) 1577
BfrI CTTAAG 1 cut(s) 1507
BfuAI ACCTGC 3 cut(s) 582, 622, 1560
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 1 cut(s) 86
BisI GCNGC 4 cut(s) 519, 1056, 1299, 1689
BlpI GCTNAGC 1 cut(s) 1577
BlsI GCNGC 4 cut(s) 520, 1057, 1300, 1690
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 5 cut(s) 374, 600, 935, 1271, 1583
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 1357
BmgT120I GGNCC 5 cut(s) 234, 596, 718, 719, 1192
BmiI GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
BmrFI CCNGG 5 cut(s) 374, 600, 935, 1271, 1583
BmrI ACTGGG 1 cut(s) 965
BmsI GCATC 3 cut(s) 8, 1095, 1382
BmtI GCTAGC 1 cut(s) 1373
BmuI ACTGGG 1 cut(s) 965
BoxI GACNNNNGTC 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 734
BpmI CTGGAG 1 cut(s) 395
Bpu10I CCTNAGC 1 cut(s) 1196
Bpu1102I GCTNAGC 1 cut(s) 1577
BpuEI CTTGAG 1 cut(s) 1223
BsaJI CCNNGG 5 cut(s) 372, 599, 714, 1000, 1425
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
Bse118I RCCGGY 2 cut(s) 556, 581
Bse1I ACTGG 2 cut(s) 461, 971
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 5 cut(s) 374, 600, 935, 1271, 1583
BseDI CCNNGG 5 cut(s) 372, 599, 714, 1000, 1425
BseLI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 3 cut(s) 801, 1074, 1650
BseNI ACTGG 2 cut(s) 461, 971
BseRI GAGGAG 3 cut(s) 124, 1584, 1694
BseSI GKGCMC 1 cut(s) 722
BseXI GCAGC 4 cut(s) 505, 1042, 1285, 1675
BseYI CCCAGC 1 cut(s) 1223
BsgI GTGCAG 1 cut(s) 650
Bsh1236I CGCG 3 cut(s) 83, 140, 183
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 6 cut(s) 5, 236, 257, 720, 1194, 1430
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 556
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 1 cut(s) 898
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 4 cut(s) 143, 150, 557, 582
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
BsmAI GTCTC 2 cut(s) 272, 1474
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 6 cut(s) 5, 236, 257, 720, 1194, 1430
BsoBI CYCGRG 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 718
Bsp1286I GDGCHC 2 cut(s) 722, 898
Bsp143I GATC 5 cut(s) 86, 178, 224, 259, 493
Bsp1720I GCTNAGC 1 cut(s) 1577
BspACI CCGC 3 cut(s) 81, 138, 990
BspANI GGCC 6 cut(s) 5, 236, 257, 720, 1194, 1430
BspCNI CTCAG 3 cut(s) 802, 1073, 1651
BspFNI CGCG 3 cut(s) 83, 140, 183
BspHI TCATGA 1 cut(s) 753
BspLI GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
BspMI ACCTGC 3 cut(s) 582, 622, 1560
BspOI GCTAGC 1 cut(s) 1373
BspPI GGATC 1 cut(s) 232
BspQI GCTCTTC 1 cut(s) 903
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 1507
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 2 cut(s) 556, 581
BsrI ACTGG 2 cut(s) 461, 971
BssAI RCCGGY 2 cut(s) 556, 581
BssECI CCNNGG 5 cut(s) 372, 599, 714, 1000, 1425
BssMI GATC 5 cut(s) 86, 178, 224, 259, 493
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 1000, 1425
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 5 cut(s) 374, 600, 935, 1271, 1583
Bst4CI ACNGT 5 cut(s) 283, 643, 651, 957, 1722
Bst6I CTCTTC 2 cut(s) 903, 1310
BstAFI CTTAAG 1 cut(s) 1507
BstC8I GCNNGC 4 cut(s) 238, 579, 1143, 1371
BstDEI CTNAG 6 cut(s) 702, 810, 1060, 1196, 1577, 1659
BstDSI CCRYGG 1 cut(s) 714
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 3 cut(s) 83, 140, 183
BstH2I RGCGCY 1 cut(s) 1577
BstHHI GCGC 4 cut(s) 65, 142, 1576, 1647
BstKTI GATC 5 cut(s) 89, 181, 227, 262, 496
BstMAI GTCTC 2 cut(s) 272, 1474
BstMBI GATC 5 cut(s) 86, 178, 224, 259, 493
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
BstNI CCWGG 5 cut(s) 374, 600, 935, 1271, 1583
BstNSI RCATGY 1 cut(s) 1337
BstPAI GACNNNNGTC 1 cut(s) 614
BstSCI CCNGG 5 cut(s) 372, 598, 933, 1269, 1581
BstSFI CTRYAG 1 cut(s) 723
BstSLI GKGCMC 1 cut(s) 722
BstUI CGCG 3 cut(s) 83, 140, 183
BstV1I GCAGC 4 cut(s) 505, 1042, 1285, 1675
BstV2I GAAGAC 1 cut(s) 734
BstX2I RGATCY 2 cut(s) 86, 224
BstYI RGATCY 2 cut(s) 86, 224
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 6 cut(s) 5, 236, 257, 720, 1194, 1430
BtgI CCRYGG 1 cut(s) 714
BtgZI GCGATG 1 cut(s) 1706
BtrI CACGTC 1 cut(s) 1357
BtsI GCAGTG 2 cut(s) 256, 861
BtsIMutI CAGTG 5 cut(s) 256, 625, 861, 1020, 1242
BveI ACCTGC 3 cut(s) 582, 622, 1560
Cac8I GCNNGC 4 cut(s) 238, 579, 1143, 1371
CciI TCATGA 1 cut(s) 753
CfoI GCGC 4 cut(s) 65, 142, 1576, 1647
Cfr10I RCCGGY 2 cut(s) 556, 581
Cfr13I GGNCC 5 cut(s) 234, 596, 718, 719, 1192
CseI GACGC 2 cut(s) 172, 1709
CsiI ACCWGGT 1 cut(s) 1269
Csp6I GTAC 4 cut(s) 272, 464, 1549, 1672
CspAI ACCGGT 1 cut(s) 556
CspCI CAANNNNNGTGG 4 cut(s) 837, 872, 1231, 1266
CviAII CATG 5 cut(s) 124, 754, 902, 1334, 1600
CviQI GTAC 4 cut(s) 272, 464, 1549, 1672
DdeI CTNAG 6 cut(s) 702, 810, 1060, 1196, 1577, 1659
DpnI GATC 5 cut(s) 88, 180, 226, 261, 495
DpnII GATC 5 cut(s) 86, 178, 224, 259, 493
DrdI GACNNNNNNGTC 1 cut(s) 603
DseDI GACNNNNNNGTC 1 cut(s) 603
EaeI YGGCCR 2 cut(s) 3, 255
Eam1104I CTCTTC 2 cut(s) 903, 1310
EarI CTCTTC 2 cut(s) 903, 1310
Eco130I CCWWGG 2 cut(s) 1000, 1425
Eco147I AGGCCT 1 cut(s) 1430
Eco24I GRGCYC 1 cut(s) 722
Eco32I GATATC 1 cut(s) 1236
Eco47I GGWCC 1 cut(s) 596
Eco47III AGCGCT 1 cut(s) 1575
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 188
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 5 cut(s) 372, 598, 933, 1269, 1581
EcoRV GATATC 1 cut(s) 1236
EcoT14I CCWWGG 2 cut(s) 1000, 1425
EcoT22I ATGCAT 1 cut(s) 1110
EcoT38I GRGCYC 1 cut(s) 722
ErhI CCWWGG 2 cut(s) 1000, 1425
FaeI CATG 5 cut(s) 127, 757, 905, 1337, 1603
FaqI GGGAC 1 cut(s) 564
FatI CATG 5 cut(s) 123, 753, 901, 1333, 1599
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 4 cut(s) 519, 1056, 1299, 1689
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 4 cut(s) 519, 1056, 1299, 1689
FspBI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
GlaI GCGC 4 cut(s) 64, 141, 1575, 1646
GluI GCNGC 4 cut(s) 519, 1056, 1299, 1689
GsaI CCCAGC 1 cut(s) 1227
GsuI CTGGAG 1 cut(s) 395
HaeII RGCGCY 1 cut(s) 1577
HaeIII GGCC 6 cut(s) 5, 236, 257, 720, 1194, 1430
HapII CCGG 4 cut(s) 143, 150, 557, 582
HgaI GACGC 2 cut(s) 172, 1709
HhaI GCGC 4 cut(s) 65, 142, 1576, 1647
Hin1II CATG 5 cut(s) 127, 757, 905, 1337, 1603
Hin6I GCGC 4 cut(s) 63, 140, 1574, 1645
HinP1I GCGC 4 cut(s) 63, 140, 1574, 1645
HincII GTYRAC 1 cut(s) 1360
HindII GTYRAC 1 cut(s) 1360
HinfI GANTC 6 cut(s) 30, 146, 386, 1467, 1612, 1627
HpaII CCGG 4 cut(s) 143, 150, 557, 582
HphI GGTGA 1 cut(s) 874
Hpy166II GTNNAC 2 cut(s) 953, 1360
Hpy188III TCNNGA 4 cut(s) 104, 190, 299, 754
Hpy8I GTNNAC 2 cut(s) 953, 1360
Hpy99I CGWCG 2 cut(s) 158, 1361
HpyAV CCTTC 6 cut(s) 187, 215, 298, 353, 841, 1665
HpyCH4III ACNGT 5 cut(s) 283, 643, 651, 957, 1722
HpyCH4IV ACGT 3 cut(s) 327, 611, 1356
HpyCH4V TGCA 5 cut(s) 631, 647, 875, 1108, 1253
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
HpyF3I CTNAG 6 cut(s) 702, 810, 1060, 1196, 1577, 1659
HpySE526I ACGT 3 cut(s) 327, 611, 1356
Hsp92II CATG 5 cut(s) 127, 757, 905, 1337, 1603
HspAI GCGC 4 cut(s) 63, 140, 1574, 1645
Kzo9I GATC 5 cut(s) 86, 178, 224, 259, 493
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 3 cut(s) 797, 972, 1350
Lsp1109I GCAGC 4 cut(s) 505, 1042, 1285, 1675
LweI GCATC 3 cut(s) 8, 1095, 1382
MabI ACCWGGT 1 cut(s) 1269
MaeI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
MaeII ACGT 3 cut(s) 327, 611, 1356
MaeIII GTNAC 5 cut(s) 910, 1038, 1286, 1420, 1585
MalI GATC 5 cut(s) 88, 180, 226, 261, 495
MboI GATC 5 cut(s) 86, 178, 224, 259, 493
MboII GAAGA 8 cut(s) 395, 404, 659, 734, 737, 890, 1297, 1550
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 2 cut(s) 86, 224
MhlI GDGCHC 2 cut(s) 722, 898
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 9 cut(s) 99, 486, 531, 816, 866, 915, 1281, 1482, 1490
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 1476, 1606
MmeI TCCRAC 2 cut(s) 310, 1444
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 1110
MroXI GAANNNNTTC 2 cut(s) 197, 1281
MscI TGGCCA 1 cut(s) 5
MseI TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
Msp20I TGGCCA 1 cut(s) 5
MspCI CTTAAG 1 cut(s) 1507
MspI CCGG 4 cut(s) 143, 150, 557, 582
MspR9I CCNGG 5 cut(s) 374, 600, 935, 1271, 1583
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 5 cut(s) 374, 600, 935, 1271, 1583
MvnI CGCG 3 cut(s) 83, 140, 183
MwoI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
NdeII GATC 5 cut(s) 86, 178, 224, 259, 493
NheI GCTAGC 1 cut(s) 1369
NlaIII CATG 5 cut(s) 127, 757, 905, 1337, 1603
NlaIV GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
NmuCI GTSAC 1 cut(s) 1038
NsiI ATGCAT 1 cut(s) 1110
NspI RCATGY 1 cut(s) 1337
PaeR7I CTCGAG 1 cut(s) 188
PagI TCATGA 1 cut(s) 753
PceI AGGCCT 1 cut(s) 1430
PciI ACATGT 1 cut(s) 1333
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 216
PctI GAATGC 1 cut(s) 1329
PdmI GAANNNNTTC 2 cut(s) 197, 1281
PfeI GAWTC 4 cut(s) 30, 146, 386, 1627
PflMI CCANNNNNTGG 1 cut(s) 1006
PfoI TCCNGGA 1 cut(s) 933
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 4 cut(s) 520, 1057, 1300, 1690
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 2 cut(s) 1475, 1606
PpsI GAGTC 2 cut(s) 1475, 1606
PscI ACATGT 1 cut(s) 1333
PshAI GACNNNNGTC 1 cut(s) 614
PshBI ATTAAT 1 cut(s) 485
Psp6I CCWGG 5 cut(s) 372, 598, 933, 1269, 1581
PspFI CCCAGC 1 cut(s) 1223
PspGI CCWGG 5 cut(s) 372, 598, 933, 1269, 1581
PspN4I GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
PspOMI GGGCCC 1 cut(s) 718
PspPI GGNCC 5 cut(s) 234, 596, 718, 719, 1192
PsuI RGATCY 2 cut(s) 86, 224
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 4 cut(s) 273, 465, 1550, 1673
RsaNI GTAC 4 cut(s) 272, 464, 1549, 1672
SalI GTCGAC 1 cut(s) 1358
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
SatI GCNGC 4 cut(s) 519, 1056, 1299, 1689
Sau3AI GATC 5 cut(s) 86, 178, 224, 259, 493
Sau96I GGNCC 5 cut(s) 234, 596, 718, 719, 1192
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 2 cut(s) 1476, 1606
ScrFI CCNGG 5 cut(s) 374, 600, 935, 1271, 1583
SduI GDGCHC 2 cut(s) 722, 898
SexAI ACCWGGT 1 cut(s) 1269
SfaNI GCATC 3 cut(s) 8, 1095, 1382
SfcI CTRYAG 1 cut(s) 723
Sfr274I CTCGAG 1 cut(s) 188
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 1 cut(s) 188
SmlI CTYRAG 3 cut(s) 188, 1202, 1507
SmoI CTYRAG 3 cut(s) 188, 1202, 1507
Sse9I AATT 9 cut(s) 99, 486, 531, 816, 866, 915, 1281, 1482, 1490
SseBI AGGCCT 1 cut(s) 1430
SsiI CCGC 3 cut(s) 81, 138, 990
SspMI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
StuI AGGCCT 1 cut(s) 1430
StyD4I CCNGG 5 cut(s) 372, 598, 933, 1269, 1581
StyI CCWWGG 2 cut(s) 1000, 1425
TaaI ACNGT 5 cut(s) 283, 643, 651, 957, 1722
TaiI ACGT 3 cut(s) 330, 614, 1359
TaqI TCGA 6 cut(s) 177, 189, 1313, 1359, 1513, 1520
TasI AATT 9 cut(s) 99, 486, 531, 816, 866, 915, 1281, 1482, 1490
TatI WGTACW 2 cut(s) 271, 463
TfiI GAWTC 4 cut(s) 30, 146, 386, 1627
Tru1I TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
Tru9I TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
TscAI CASTG 5 cut(s) 256, 625, 861, 1020, 1249
TseFI GTSAC 1 cut(s) 1038
TseI GCWGC 4 cut(s) 518, 1055, 1298, 1688
Tsp45I GTSAC 1 cut(s) 1038
TspDTI ATGAA 5 cut(s) 396, 890, 1203, 1328, 1640
TspGWI ACGGA 1 cut(s) 1659
TspRI CASTG 5 cut(s) 256, 625, 861, 1020, 1249
Van91I CCANNNNNTGG 1 cut(s) 1006
Vha464I CTTAAG 1 cut(s) 1507
VpaK11BI GGWCC 1 cut(s) 596
VspI ATTAAT 1 cut(s) 485
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 5 cut(s) 99, 531, 816, 866, 1490
XceI RCATGY 1 cut(s) 1337
XhoI CTCGAG 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 1359
XmnI GAANNNNTTC 2 cut(s) 197, 1281
XspI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
ZrmI AGTACT 1 cut(s) 465
Zsp2I ATGCAT 1 cut(s) 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.