MD07G1259700.v1.1

) efflux antiporter

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0007412
Physical Location & Seq
Reverse (-)
32588683 .. 32600428
11746 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1259700.v1.1.491

Sequence Viewer

Length: 1725 bp
ATGGCGAAGAGAAGACGATGCGTGTTTGGATTCTGCTATTCTCTTTTGACGATTTTGGTTTACGCTAGGGTTTCGTTTTCCGCGAGATCTGATAAGGAAGTCAGGGAGAGGTTCTACGGCAACATGATGAACTCCTCCGCGCCGGTTTCCGGCGACGGCACCATTGCCAAAATGTTCGATCGCGTCCTCGAGAAGGAGTTCTCCGAGAACGATCAGCCCGAAGGATCTGATGGGGCCAGCTTTAACAGCAGTGTGGCCGATCAACAAGCTGTACTAGAGACTGTGGCGAAAATCACTCACGAGAAGGGCAAGAAAAATGATACACAAGTGGGAAACGGCACGAAGCCTTATGATTTTGAAGGTGTTTTTTCATTGGAGAATGAAGATTCTGAAGAAACAACAACCTTGATTGACAAAAAGGATAATGTGTTTGTGATGTCAAACAAGAAATCTAAATATCCAGTACTTCAAGTAGATTTAAGATTAATTTCTGATCTGGTGGTTATCATAGTATCTGCTGCCATTGGTGGAATTCTGTTTTCTTGTTTGGGACAACCGGTTATTGTCGGATACCTGCTTGCTGGTTCTCTCATTGGACCTGGAGGTCTTACAATCATCACTGAAATGGTGCAGGTTGAAACTGTTGCACAGTTTGGTGTTGTATTTCTTCTTTTTGCTCTAGGTTTGGAGTTTTCTTTGGCTAAGTTAAAAGCTGTGGGCCCTGTAGCTGTTCTTGGAGGCCTTCTTCAAATAATGACATTTATGCTCTTGTGTGCCATAACTGCTGTGTTATGTGGAGCAAAGTTGTCTGAGGGAATTTTTGTCGGTTCCTTCCTTTCAATGTCCTCAACAGCGGTGGTGGTGAAATTTCTTGCAGAAAGGAATAGTAATAGTGCTCTTCACGGTCAAGTAACAATTGGAACGCTCATTTTCCAGGACTGTGCTGTTGGTTTATTGTTTGCTTTGCTCCCAGTTTTGGGTGGTCATAGCGGCCTTTTCCAAGGAATGGTATCAGTAGGGAAGCTGTTGCTGGTGCTGTCACTATATCTTGGTGCTGTGTCGGTATTCTGTTGGTCATTTGTTCCTCGCTTTCTGAAACTGATGATGCAGTTGTCCTCTCAAACAAATGAGCTCTATCAGCTTGCTGTTGTGGCTTTCTGCTTATTATCTGCTTGGTGCAGTGATAAGTTGGGCCTTAGCCTTGAGTTGGGTTCATTTGTGGCTGGAGTTATGATATCAACCACTGACCTTGCACAACATACTTTAGACCAGGTGGAACCAATTCGTAACCTATTTGCAGCTCTCTTCCTTTCAAGTATTGGAATGCTCATACATGTACATTTCTTGTGGAGCCACGTCGACATACTGCTAGCATCTGTAATTCTAGTTATAGTTGTAAAGACTGCTGTTGCTACTATTGTTACTAAGGCCTTTGGATATAGCTTTAGGACGTCATTTGTGGTTGGAGTCTCACTTGCTCAAATTGGAGAATTTGCTTTTGTCCTCTTAAGTCGGGCCTCAAATCTTAAACTTGTTGAGGGAAAAATGTACCTGCTTCTTCTCGGAACAACAGCGCTTAGCCTGGTTACAACTCCTCTCTTGTTTAAGTTGACTCCCGCCATAATGAACCTAGGAGTTCTTATGCACTGGTTTCCTTCCGAGGGTACTCCATATAACGAGGAGAAAGCTTCAATGATCGACGCACAGCACAACCGCATATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005215 GO:0005575 GO:0006810 GO:0006811 GO:0006812 GO:0008150 GO:0008324 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0034220 GO:0051179 GO:0051234 GO:0055085 GO:0098655
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Na_H_Exchanger

Protein Analysis

575

Amino Acids

62.07

Weight (kDa)

6.33

Isoelectric Point (pI)

27.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Na_H_Exchanger PF00999 170 - 536 3.6e-74 Sodium/hydrogen exchanger, transmembrane
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 603
AatII GACGTC 1 cut(s) 1454
Acc36I ACCTGC 3 cut(s) 582, 622, 1560
AccB1I GGYRCC 1 cut(s) 158
AccB7I CCANNNNNTGG 1 cut(s) 1006
AccI GTMKAC 1 cut(s) 1359
AccII CGCG 3 cut(s) 83, 140, 183
AciI CCGC 6 cut(s) 81, 138, 854, 990, 1617, 1714
AclWI GGATC 1 cut(s) 232
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 4 cut(s) 531, 816, 866, 1490
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 1451
AfaI GTAC 5 cut(s) 273, 465, 1338, 1550, 1666
AfeI AGCGCT 1 cut(s) 1575
AfiI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
AflII CTTAAG 1 cut(s) 1507
AflIII ACRYGT 1 cut(s) 1333
AgeI ACCGGT 1 cut(s) 556
AgsI TTSAA 7 cut(s) 359, 470, 638, 749, 840, 1314, 1692
AjiI CACGTC 1 cut(s) 1357
AjnI CCWGG 4 cut(s) 598, 933, 1269, 1581
Alw21I GWGCWC 2 cut(s) 898, 1134
Alw26I GTCTC 2 cut(s) 272, 1474
AlwI GGATC 1 cut(s) 232
Ama87I CYCGRG 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 1575
AoxI GGCC 8 cut(s) 234, 255, 718, 739, 991, 1192, 1428, 1515
ApaI GGGCCC 1 cut(s) 722
ApeKI GCWGC 2 cut(s) 518, 1298
ApoI RAATTY 4 cut(s) 531, 816, 866, 1490
AseI ATTAAT 1 cut(s) 485
AsiGI ACCGGT 1 cut(s) 556
Asp700I GAANNNNTTC 2 cut(s) 197, 1281
AspA2I CCTAGG 1 cut(s) 1630
AspLEI GCGC 2 cut(s) 142, 1576
AspS9I GGNCC 6 cut(s) 234, 596, 718, 719, 1192, 1515
AsuHPI GGTGA 1 cut(s) 874
AsuNHI GCTAGC 1 cut(s) 1369
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 1 cut(s) 596
AvrII CCTAGG 1 cut(s) 1630
BaeGI GKGCMC 1 cut(s) 722
BaeI ACNNNNGTAYC 2 cut(s) 312, 345
BanI GGYRCC 1 cut(s) 158
BanII GRGCYC 2 cut(s) 722, 1134
BauI CACGAG 1 cut(s) 299
BbsI GAAGAC 1 cut(s) 19
Bbv12I GWGCWC 2 cut(s) 898, 1134
BbvI GCAGC 2 cut(s) 505, 1310
BccI CCATC 1 cut(s) 224
BceAI ACGGC 3 cut(s) 133, 172, 352
BciT130I CCWGG 4 cut(s) 600, 935, 1271, 1583
BciVI GTATCC 1 cut(s) 563
BcoDI GTCTC 2 cut(s) 272, 1474
BfaI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
BfmI CTRYAG 1 cut(s) 723
BfoI RGCGCY 1 cut(s) 1577
BfrI CTTAAG 1 cut(s) 1507
BfuAI ACCTGC 3 cut(s) 582, 622, 1560
BfuI GTATCC 1 cut(s) 563
BglII AGATCT 1 cut(s) 86
BisI GCNGC 3 cut(s) 519, 991, 1299
BlnI CCTAGG 1 cut(s) 1630
BlpI GCTNAGC 1 cut(s) 1577
BlsI GCNGC 3 cut(s) 520, 992, 1300
BmcAI AGTACT 1 cut(s) 465
Bme1390I CCNGG 4 cut(s) 600, 935, 1271, 1583
Bme18I GGWCC 1 cut(s) 596
BmeT110I CYCGRG 1 cut(s) 188
BmgBI CACGTC 1 cut(s) 1357
BmgT120I GGNCC 6 cut(s) 234, 596, 718, 719, 1192, 1515
BmiI GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
BmrFI CCNGG 4 cut(s) 600, 935, 1271, 1583
BmrI ACTGGG 1 cut(s) 965
BmsI GCATC 3 cut(s) 8, 1095, 1382
BmtI GCTAGC 1 cut(s) 1373
BmuI ACTGGG 1 cut(s) 965
BpiI GAAGAC 1 cut(s) 19
BpmI CTGGAG 2 cut(s) 621, 1245
Bpu10I CCTNAGC 1 cut(s) 1196
Bpu1102I GCTNAGC 1 cut(s) 1577
BpuEI CTTGAG 1 cut(s) 1223
BsaHI GRCGYC 1 cut(s) 1451
BsaJI CCNNGG 3 cut(s) 1000, 1630, 1659
BsaWI WCCGGW 1 cut(s) 556
Bsc4I CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
Bse118I RCCGGY 2 cut(s) 142, 556
Bse1I ACTGG 3 cut(s) 461, 971, 1652
Bse3DI GCAATG 1 cut(s) 162
BseBI CCWGG 4 cut(s) 600, 935, 1271, 1583
BseDI CCNNGG 3 cut(s) 1000, 1630, 1659
BseLI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 1 cut(s) 801
BseNI ACTGG 3 cut(s) 461, 971, 1652
BseRI GAGGAG 3 cut(s) 124, 1584, 1694
BseSI GKGCMC 1 cut(s) 722
BseXI GCAGC 2 cut(s) 505, 1310
BsgI GTGCAG 2 cut(s) 650, 1198
Bsh1236I CGCG 3 cut(s) 83, 140, 183
Bsh1285I CGRYCG 1 cut(s) 181
BshFI GGCC 8 cut(s) 236, 257, 720, 741, 993, 1194, 1430, 1517
BshNI GGYRCC 1 cut(s) 158
BshTI ACCGGT 1 cut(s) 556
BsiEI CGRYCG 1 cut(s) 181
BsiHKAI GWGCWC 2 cut(s) 898, 1134
BsiHKCI CYCGRG 1 cut(s) 188
BsiSI CCGG 3 cut(s) 143, 150, 557
BslFI GGGAC 1 cut(s) 564
BslI CCNNNNNNNGG 7 cut(s) 149, 193, 976, 977, 1006, 1207, 1661
BsmAI GTCTC 2 cut(s) 272, 1474
BsmFI GGGAC 1 cut(s) 564
BsmI GAATGC 1 cut(s) 1329
BsnI GGCC 8 cut(s) 236, 257, 720, 741, 993, 1194, 1430, 1517
BsoBI CYCGRG 1 cut(s) 188
Bsp120I GGGCCC 1 cut(s) 718
Bsp1286I GDGCHC 3 cut(s) 722, 898, 1134
Bsp1407I TGTACA 1 cut(s) 1336
Bsp143I GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
Bsp1720I GCTNAGC 1 cut(s) 1577
BspACI CCGC 6 cut(s) 81, 138, 854, 990, 1617, 1714
BspANI GGCC 8 cut(s) 236, 257, 720, 741, 993, 1194, 1430, 1517
BspCNI CTCAG 1 cut(s) 802
BspFNI CGCG 3 cut(s) 83, 140, 183
BspLI GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
BspMI ACCTGC 3 cut(s) 582, 622, 1560
BspOI GCTAGC 1 cut(s) 1373
BspPI GGATC 1 cut(s) 232
BspQI GCTCTTC 1 cut(s) 903
BspT107I GGYRCC 1 cut(s) 158
BspTI CTTAAG 1 cut(s) 1507
BsrDI GCAATG 1 cut(s) 162
BsrFI RCCGGY 2 cut(s) 142, 556
BsrGI TGTACA 1 cut(s) 1336
BsrI ACTGG 3 cut(s) 461, 971, 1652
BssAI RCCGGY 2 cut(s) 142, 556
BssECI CCNNGG 3 cut(s) 1000, 1630, 1659
BssMI GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
BssNI GRCGYC 1 cut(s) 1451
BssSI CACGAG 1 cut(s) 299
BssT1I CCWWGG 2 cut(s) 1000, 1630
Bst2BI CACGAG 1 cut(s) 299
Bst2UI CCWGG 4 cut(s) 600, 935, 1271, 1583
Bst4CI ACNGT 5 cut(s) 283, 643, 651, 905, 941
Bst6I CTCTTC 3 cut(s) 2, 903, 1310
BstACI GRCGYC 1 cut(s) 1451
BstAFI CTTAAG 1 cut(s) 1507
BstAUI TGTACA 1 cut(s) 1336
BstC8I GCNNGC 4 cut(s) 238, 579, 1143, 1371
BstDEI CTNAG 5 cut(s) 702, 810, 1196, 1425, 1577
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 3 cut(s) 83, 140, 183
BstH2I RGCGCY 1 cut(s) 1577
BstHHI GCGC 2 cut(s) 142, 1576
BstKTI GATC 7 cut(s) 89, 181, 214, 227, 262, 496, 1698
BstMAI GTCTC 2 cut(s) 272, 1474
BstMBI GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
BstMCI CGRYCG 1 cut(s) 181
BstMWI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
BstNI CCWGG 4 cut(s) 600, 935, 1271, 1583
BstNSI RCATGY 1 cut(s) 1337
BstSCI CCNGG 4 cut(s) 598, 933, 1269, 1581
BstSFI CTRYAG 1 cut(s) 723
BstSLI GKGCMC 1 cut(s) 722
BstUI CGCG 3 cut(s) 83, 140, 183
BstV1I GCAGC 2 cut(s) 505, 1310
BstV2I GAAGAC 1 cut(s) 19
BstX2I RGATCY 2 cut(s) 86, 224
BstYI RGATCY 2 cut(s) 86, 224
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 8 cut(s) 236, 257, 720, 741, 993, 1194, 1430, 1517
BtrI CACGTC 1 cut(s) 1357
BtsI GCAGTG 2 cut(s) 256, 1186
BtsIMutI CAGTG 5 cut(s) 256, 618, 1186, 1242, 1645
BveI ACCTGC 3 cut(s) 582, 622, 1560
Cac8I GCNNGC 4 cut(s) 238, 579, 1143, 1371
CfoI GCGC 2 cut(s) 142, 1576
Cfr10I RCCGGY 2 cut(s) 142, 556
Cfr13I GGNCC 6 cut(s) 234, 596, 718, 719, 1192, 1515
CseI GACGC 2 cut(s) 172, 1709
CsiI ACCWGGT 1 cut(s) 1269
Csp6I GTAC 5 cut(s) 272, 464, 1337, 1549, 1665
CspAI ACCGGT 1 cut(s) 556
CspCI CAANNNNNGTGG 4 cut(s) 837, 872, 1231, 1266
CviAII CATG 2 cut(s) 124, 1334
CviQI GTAC 5 cut(s) 272, 464, 1337, 1549, 1665
DdeI CTNAG 5 cut(s) 702, 810, 1196, 1425, 1577
DpnI GATC 7 cut(s) 88, 180, 213, 226, 261, 495, 1697
DpnII GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
DrdI GACNNNNNNGTC 1 cut(s) 603
DseDI GACNNNNNNGTC 1 cut(s) 603
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 2, 903, 1310
EarI CTCTTC 3 cut(s) 2, 903, 1310
Ecl136II GAGCTC 1 cut(s) 1132
Eco130I CCWWGG 2 cut(s) 1000, 1630
Eco147I AGGCCT 2 cut(s) 741, 1430
Eco24I GRGCYC 2 cut(s) 722, 1134
Eco32I GATATC 1 cut(s) 1236
Eco47I GGWCC 1 cut(s) 596
Eco47III AGCGCT 1 cut(s) 1575
Eco53kI GAGCTC 1 cut(s) 1132
Eco57I CTGAAG 1 cut(s) 411
Eco88I CYCGRG 1 cut(s) 188
EcoICRI GAGCTC 1 cut(s) 1132
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 1 cut(s) 719
EcoRI GAATTC 1 cut(s) 531
EcoRII CCWGG 4 cut(s) 598, 933, 1269, 1581
EcoRV GATATC 1 cut(s) 1236
EcoT14I CCWWGG 2 cut(s) 1000, 1630
EcoT38I GRGCYC 2 cut(s) 722, 1134
ErhI CCWWGG 2 cut(s) 1000, 1630
FaeI CATG 2 cut(s) 127, 1337
FaqI GGGAC 1 cut(s) 564
FatI CATG 2 cut(s) 123, 1333
FauI CCCGC 1 cut(s) 1624
FblI GTMKAC 1 cut(s) 1359
Fnu4HI GCNGC 3 cut(s) 519, 991, 1299
FriOI GRGCYC 2 cut(s) 722, 1134
Fsp4HI GCNGC 3 cut(s) 519, 991, 1299
FspBI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
GlaI GCGC 2 cut(s) 141, 1575
GluI GCNGC 3 cut(s) 519, 991, 1299
GsuI CTGGAG 2 cut(s) 621, 1245
HaeII RGCGCY 1 cut(s) 1577
HaeIII GGCC 8 cut(s) 236, 257, 720, 741, 993, 1194, 1430, 1517
HapII CCGG 3 cut(s) 143, 150, 557
HgaI GACGC 2 cut(s) 172, 1709
HhaI GCGC 2 cut(s) 142, 1576
Hin1I GRCGYC 1 cut(s) 1451
Hin1II CATG 2 cut(s) 127, 1337
Hin6I GCGC 2 cut(s) 140, 1574
HinP1I GCGC 2 cut(s) 140, 1574
HincII GTYRAC 2 cut(s) 1360, 1611
HindII GTYRAC 2 cut(s) 1360, 1611
HindIII AAGCTT 1 cut(s) 1686
HinfI GANTC 4 cut(s) 30, 386, 1467, 1612
HpaII CCGG 3 cut(s) 143, 150, 557
HphI GGTGA 1 cut(s) 874
Hpy166II GTNNAC 3 cut(s) 61, 1360, 1611
Hpy188III TCNNGA 2 cut(s) 190, 299
Hpy8I GTNNAC 3 cut(s) 61, 1360, 1611
Hpy99I CGWCG 3 cut(s) 158, 1361, 1703
HpyAV CCTTC 7 cut(s) 187, 215, 298, 353, 752, 841, 1665
HpyCH4III ACNGT 5 cut(s) 283, 643, 651, 905, 941
HpyCH4IV ACGT 2 cut(s) 1356, 1451
HpyCH4V TGCA 8 cut(s) 631, 647, 875, 1108, 1179, 1253, 1298, 1645
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
HpyF3I CTNAG 5 cut(s) 702, 810, 1196, 1425, 1577
HpySE526I ACGT 2 cut(s) 1356, 1451
Hsp92I GRCGYC 1 cut(s) 1451
Hsp92II CATG 2 cut(s) 127, 1337
HspAI GCGC 2 cut(s) 140, 1574
Kzo9I GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
LguI GCTCTTC 1 cut(s) 903
LmnI GCTCC 3 cut(s) 797, 972, 1350
Lsp1109I GCAGC 2 cut(s) 505, 1310
LweI GCATC 3 cut(s) 8, 1095, 1382
MabI ACCWGGT 1 cut(s) 1269
MaeI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
MaeII ACGT 2 cut(s) 1356, 1451
MaeIII GTNAC 5 cut(s) 910, 1038, 1286, 1420, 1585
MalI GATC 7 cut(s) 88, 180, 213, 226, 261, 495, 1697
MboI GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
MboII GAAGA 9 cut(s) 19, 24, 395, 404, 659, 737, 890, 1297, 1550
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 2 cut(s) 86, 224
MhlI GDGCHC 3 cut(s) 722, 898, 1134
MluCI AATT 9 cut(s) 486, 531, 816, 866, 915, 1281, 1380, 1482, 1490
MlyI GAGTC 2 cut(s) 1476, 1606
MmeI TCCRAC 2 cut(s) 547, 1444
MroXI GAANNNNTTC 2 cut(s) 197, 1281
MseI TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
MslI CAYNNNNRTG 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 854
MspCI CTTAAG 1 cut(s) 1507
MspI CCGG 3 cut(s) 143, 150, 557
MspR9I CCNGG 4 cut(s) 600, 935, 1271, 1583
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 1329
MvaI CCWGG 4 cut(s) 600, 935, 1271, 1583
MvnI CGCG 3 cut(s) 83, 140, 183
MwoI GCNNNNNNNGC 4 cut(s) 246, 782, 1138, 1151
NdeII GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
NheI GCTAGC 1 cut(s) 1369
NlaIII CATG 2 cut(s) 127, 1337
NlaIV GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
NmuCI GTSAC 1 cut(s) 1038
NspI RCATGY 1 cut(s) 1337
PaeR7I CTCGAG 1 cut(s) 188
PceI AGGCCT 2 cut(s) 741, 1430
PciI ACATGT 1 cut(s) 1333
PciSI GCTCTTC 1 cut(s) 903
PcsI WCGNNNNNNNCGW 2 cut(s) 80, 216
PctI GAATGC 1 cut(s) 1329
PdmI GAANNNNTTC 2 cut(s) 197, 1281
PfeI GAWTC 2 cut(s) 30, 386
PflMI CCANNNNNTGG 1 cut(s) 1006
PfoI TCCNGGA 1 cut(s) 933
PinAI ACCGGT 1 cut(s) 556
PkrI GCNGC 3 cut(s) 520, 992, 1300
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 2 cut(s) 1475, 1606
PpsI GAGTC 2 cut(s) 1475, 1606
PscI ACATGT 1 cut(s) 1333
PshBI ATTAAT 1 cut(s) 485
Psp124BI GAGCTC 1 cut(s) 1134
Psp6I CCWGG 4 cut(s) 598, 933, 1269, 1581
PspGI CCWGG 4 cut(s) 598, 933, 1269, 1581
PspN4I GGNNCC 6 cut(s) 160, 235, 720, 829, 1278, 1352
PspOMI GGGCCC 1 cut(s) 718
PspPI GGNCC 6 cut(s) 234, 596, 718, 719, 1192, 1515
PsuI RGATCY 2 cut(s) 86, 224
PvuI CGATCG 1 cut(s) 181
RsaI GTAC 5 cut(s) 273, 465, 1338, 1550, 1666
RsaNI GTAC 5 cut(s) 272, 464, 1337, 1549, 1665
RseI CAYNNNNRTG 1 cut(s) 623
SacI GAGCTC 1 cut(s) 1134
SalI GTCGAC 1 cut(s) 1358
SapI GCTCTTC 1 cut(s) 903
SaqAI TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
SatI GCNGC 3 cut(s) 519, 991, 1299
Sau3AI GATC 7 cut(s) 86, 178, 211, 224, 259, 493, 1695
Sau96I GGNCC 6 cut(s) 234, 596, 718, 719, 1192, 1515
ScaI AGTACT 1 cut(s) 465
SchI GAGTC 2 cut(s) 1476, 1606
ScrFI CCNGG 4 cut(s) 600, 935, 1271, 1583
SduI GDGCHC 3 cut(s) 722, 898, 1134
SexAI ACCWGGT 1 cut(s) 1269
SfaNI GCATC 3 cut(s) 8, 1095, 1382
SfcI CTRYAG 1 cut(s) 723
Sfr274I CTCGAG 1 cut(s) 188
SinI GGWCC 1 cut(s) 596
SlaI CTCGAG 1 cut(s) 188
SmiMI CAYNNNNRTG 1 cut(s) 623
SmlI CTYRAG 3 cut(s) 188, 1202, 1507
SmoI CTYRAG 3 cut(s) 188, 1202, 1507
Sse9I AATT 9 cut(s) 486, 531, 816, 866, 915, 1281, 1380, 1482, 1490
SseBI AGGCCT 2 cut(s) 741, 1430
SsiI CCGC 6 cut(s) 81, 138, 854, 990, 1617, 1714
SspMI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
SstI GAGCTC 1 cut(s) 1134
StuI AGGCCT 2 cut(s) 741, 1430
StyD4I CCNGG 4 cut(s) 598, 933, 1269, 1581
StyI CCWWGG 2 cut(s) 1000, 1630
TaaI ACNGT 5 cut(s) 283, 643, 651, 905, 941
TaiI ACGT 2 cut(s) 1359, 1454
TaqI TCGA 4 cut(s) 177, 189, 1359, 1698
TasI AATT 9 cut(s) 486, 531, 816, 866, 915, 1281, 1380, 1482, 1490
TatI WGTACW 3 cut(s) 271, 463, 1336
TauI GCSGC 1 cut(s) 993
TfiI GAWTC 2 cut(s) 30, 386
Tru1I TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
Tru9I TTAA 7 cut(s) 243, 479, 485, 707, 1508, 1527, 1605
TscAI CASTG 5 cut(s) 256, 625, 1186, 1249, 1652
TseFI GTSAC 1 cut(s) 1038
TseI GCWGC 2 cut(s) 518, 1298
Tsp45I GTSAC 1 cut(s) 1038
TspDTI ATGAA 5 cut(s) 143, 360, 396, 1203, 1640
TspRI CASTG 5 cut(s) 256, 625, 1186, 1249, 1652
Van91I CCANNNNNTGG 1 cut(s) 1006
Vha464I CTTAAG 1 cut(s) 1507
VpaK11BI GGWCC 1 cut(s) 596
VspI ATTAAT 1 cut(s) 485
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 4 cut(s) 531, 816, 866, 1490
XceI RCATGY 1 cut(s) 1337
XhoI CTCGAG 1 cut(s) 188
XmaJI CCTAGG 1 cut(s) 1630
XmiI GTMKAC 1 cut(s) 1359
XmnI GAANNNNTTC 2 cut(s) 197, 1281
XspI CTAG 6 cut(s) 66, 275, 680, 1370, 1385, 1631
ZraI GACGTC 1 cut(s) 1452
ZrmI AGTACT 1 cut(s) 465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.