AT5G54860

BT1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013256
Physical Location & Seq
Forward (+)
22284425 .. 22287206
2782 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54860.1

Sequence Viewer

Length: 1476 bp
ATGATACATTGGTTGAAGCAGCTGCGATCGGCGTTTGGCGTCGCGTTTCTGTGGCTCGTTTGTCTCATTTACTTCACTCAGGGATTTAGATCGTTTGTGTGGACAGCTGTTTCGTACCAGCTCAAAGACAGGCTACAGTTATCACCATCAGCTTCTCAGTTTGTCTTTTCGGTTGCCTTTTTTCCATGGAGCATAAAACCATTATATGGAATCATTTCAGATTGTATACCAATTGGAGGGAAAAAGAGGACGCCGTATTTGGTGATATCGACTGTGCTCTCTCTTGTGCCATGGCTCGTGCTTGGTCTAGATTCAACCTCGAGAAGTTCAAGTCTCTATCTGATGATTTTCTTGACTGTACAAAATCTAGGATCAGCTATGGCTGATGTTGTGATTGATGCTATGATAGCTGAGGCAGTAAGACTTGAAAAGGCTTCGTTTGCTGGAGATCTTCAGTCTGTATCATGGTTTGCAATGGCTGTGGGTGGAGTATGCGGTAGTTTATTAGGAGGCTATGCGTTATCTAACTTGAAGATAGAAACAATATTCCTCCTTTTCACGGTACTACCAGCGTTACAATTACTATCATGTGCTCTCGTCGAAGAAAGTCCTGCTAACAATGAACCATTGCCTGAGTTGTTGGATTCAAACGAGTTTGAAGAGAAAAGCTTGACGAGCAATGATAACTATCCAGATACGAGTAAGTCCAATACAAGAAGAAGGAAGGGGCAGAAGAAAGGTAAAAAAGGAGATTCCAACGGAAAGTCTGAGACACAGAAGAAGCAATCGAAGTCTTTAGCTTCACAGTGGTTCCAGTCACTAAAAGCAGCCACTTTTGGTTTGGGCCGTGCGTTTAAGCAACCGATCATTTTGAGACCTATGGCATGGTTTTTCATTGCACATATCACTGTGCCAAATCTCTCTACCGTCATGTTCTATTACCAAACCGAGGTCTTGCAATTAGACGCTGCTTTCCTAGGAACTGCCCGTGTTGTTGGTTGGTTAGGTCTCATGTTTGGAACCTTTATCTACAATCGCTATCTGCAAGATATGACTCTACGCAAGTCTCTCCTGTTTGCTCACATCGGGCTGTCTGTAACGATACTCCTAGATATGGTTCTAGTATCAAGAGCTAATGTGGGCTACGGAGTTTCAGACAAGACAATGGTGCTCTTCGGATCAGCTCTAGGTGATGCCATCAATCAACTCAAATTCATGCCGTTCCTGATCTTGTCTGGTCGTCTATGTCCACCTGGGATTGAAGGAACACTGTTTGCGCTGTTTATGTCGATAAACAACCTAGGAAACACAGTTGGATCGTTCATGGGAGCAGGATTGGCTTCACTTCTGGGAATATCGTCAGGGTCGTTTGACAATATGTTTATGGGCTTAGCCATTCAAGTGTTTTGCACTTATATACCCGTTTTGTTTCTTTTCTTGATTCCCAAAGAAGCTACAGGAGTATCAGCATCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

491

Amino Acids

53.88

Weight (kDa)

9.22

Isoelectric Point (pI)

43.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 25 - 439 9.5e-23 Major Facilitator Superfamily
BT1 PF03092 27 - 483 6e-102 BT1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54860
fragaria_vesca FvH4_3g22370 FvH4_3g22370 FvH4_3g22370 FvH4_3g22370
malus_domestica MD03G1205600.v1.1
prunus_persica Prupe.4G210100_v2.0.a1
pyrus_communis pycom03g15530
rosa_chinensis RchiOBHm_Chr5g0039011
rosa_laevigata RLG00000033883
rosa_multiflora Rmu_sc0041879.1_g000001
rosa_roxburghii Rroxscaffold_1G00042110
rosa_rugosa Rorug05G0175600
rosa_samantha Rh5AG262300 Rh5BG266900 Rh5CG299500 Rh5DG273400
rosa_wichuraiana Rw5G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 1 cut(s) 226
AccII CGCG 1 cut(s) 44
AciI CCGC 1 cut(s) 495
AclWI GGATC 3 cut(s) 379, 1186, 1324
AcsI RAATTY 1 cut(s) 1211
AcuI CTGAAG 1 cut(s) 437
AcyI GRCGYC 2 cut(s) 39, 251
AfaI GTAC 3 cut(s) 116, 360, 564
AfiI CCNNNNNNNGG 5 cut(s) 206, 236, 559, 949, 1114
AgsI TTSAA 9 cut(s) 16, 315, 330, 428, 532, 648, 659, 1262, 1400
AjnI CCWGG 1 cut(s) 1252
Alw21I GWGCWC 3 cut(s) 279, 595, 1173
Alw26I GTCTC 6 cut(s) 68, 338, 764, 868, 1013, 1071
AlwI GGATC 3 cut(s) 379, 1186, 1324
Ama87I CYCGRG 1 cut(s) 319
AoxI GGCC 1 cut(s) 844
ApeKI GCWGC 4 cut(s) 19, 22, 827, 968
ApoI RAATTY 1 cut(s) 1211
ArsI GACNNNNNNTTYG 4 cut(s) 94, 126, 241, 273
Asp700I GAANNNNTTC 1 cut(s) 214
AspA2I CCTAGG 2 cut(s) 976, 1300
AspLEI GCGC 1 cut(s) 1279
AspS9I GGNCC 1 cut(s) 844
AsuHPI GGTGA 3 cut(s) 135, 274, 1202
AvaI CYCGRG 1 cut(s) 319
AvrII CCTAGG 2 cut(s) 976, 1300
BaeI ACNNNNGTAYC 2 cut(s) 29, 1446
BauI CACGAG 1 cut(s) 296
Bbv12I GWGCWC 3 cut(s) 279, 595, 1173
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 4 cut(s) 9, 31, 839, 955
BccI CCATC 2 cut(s) 154, 1205
BceAI ACGGC 3 cut(s) 238, 831, 1204
BciT130I CCWGG 1 cut(s) 1254
BcoDI GTCTC 6 cut(s) 68, 338, 764, 868, 1013, 1071
BfaI CTAG 7 cut(s) 308, 368, 977, 1109, 1121, 1187, 1301
BfmI CTRYAG 2 cut(s) 134, 1455
BglII AGATCT 1 cut(s) 448
BisI GCNGC 4 cut(s) 20, 23, 828, 969
BlnI CCTAGG 2 cut(s) 976, 1300
BlpI GCTNAGC 1 cut(s) 1390
BlsI GCNGC 4 cut(s) 21, 24, 829, 970
Bme1390I CCNGG 1 cut(s) 1254
BmeT110I CYCGRG 1 cut(s) 319
BmgT120I GGNCC 1 cut(s) 844
BmiI GGNNCC 2 cut(s) 812, 1021
BmrFI CCNGG 1 cut(s) 1254
BmsI GCATC 2 cut(s) 388, 1183
BpmI CTGGAG 1 cut(s) 465
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 1390
BsaBI GATNNNNATC 2 cut(s) 88, 687
BsaHI GRCGYC 2 cut(s) 39, 251
BsaI GGTCTC 2 cut(s) 868, 1013
BsaJI CCNNGG 6 cut(s) 185, 290, 948, 976, 1253, 1300
Bsc4I CCNNNNNNNGG 5 cut(s) 206, 236, 559, 949, 1114
Bse1I ACTGG 1 cut(s) 814
Bse3DI GCAATG 4 cut(s) 480, 626, 685, 894
Bse8I GATNNNNATC 2 cut(s) 88, 687
BseBI CCWGG 1 cut(s) 1254
BseDI CCNNGG 6 cut(s) 185, 290, 948, 976, 1253, 1300
BseJI GATNNNNATC 2 cut(s) 88, 687
BseLI CCNNNNNNNGG 5 cut(s) 206, 236, 559, 949, 1114
BseMI GCAATG 4 cut(s) 480, 626, 685, 894
BseMII CTCAG 5 cut(s) 92, 170, 402, 624, 759
BseNI ACTGG 1 cut(s) 814
BseXI GCAGC 4 cut(s) 9, 31, 839, 955
Bsh1236I CGCG 1 cut(s) 44
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 846
BsiEI CGRYCG 1 cut(s) 29
BsiHKAI GWGCWC 3 cut(s) 279, 595, 1173
BsiHKCI CYCGRG 1 cut(s) 319
BslI CCNNNNNNNGG 5 cut(s) 206, 236, 559, 949, 1114
BsmAI GTCTC 6 cut(s) 68, 338, 764, 868, 1013, 1071
BsnI GGCC 1 cut(s) 846
Bso31I GGTCTC 2 cut(s) 868, 1013
BsoBI CYCGRG 1 cut(s) 319
Bsp1286I GDGCHC 3 cut(s) 279, 595, 1173
Bsp1407I TGTACA 1 cut(s) 358
Bsp143I GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
Bsp1720I GCTNAGC 1 cut(s) 1390
Bsp19I CCATGG 2 cut(s) 185, 290
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 846
BspCNI CTCAG 5 cut(s) 91, 169, 403, 625, 760
BspFNI CGCG 1 cut(s) 44
BspLI GGNNCC 2 cut(s) 812, 1021
BspPI GGATC 3 cut(s) 379, 1186, 1324
BspQI GCTCTTC 1 cut(s) 1178
BspTNI GGTCTC 2 cut(s) 868, 1013
BsrDI GCAATG 4 cut(s) 480, 626, 685, 894
BsrGI TGTACA 1 cut(s) 358
BsrI ACTGG 1 cut(s) 814
BssECI CCNNGG 6 cut(s) 185, 290, 948, 976, 1253, 1300
BssMI GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
BssNAI GTATAC 1 cut(s) 227
BssNI GRCGYC 2 cut(s) 39, 251
BssSI CACGAG 1 cut(s) 296
BssT1I CCWWGG 4 cut(s) 185, 290, 976, 1300
Bst1107I GTATAC 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 296
Bst2UI CCWGG 1 cut(s) 1254
Bst4CI ACNGT 9 cut(s) 138, 274, 358, 562, 807, 910, 928, 1272, 1312
Bst6I CTCTTC 2 cut(s) 654, 1178
BstACI GRCGYC 2 cut(s) 39, 251
BstAUI TGTACA 1 cut(s) 358
BstDEI CTNAG 6 cut(s) 78, 156, 411, 633, 768, 1390
BstDSI CCRYGG 2 cut(s) 185, 290
BstFNI CGCG 1 cut(s) 44
BstHHI GCGC 1 cut(s) 1279
BstKTI GATC 8 cut(s) 29, 92, 374, 451, 867, 1181, 1230, 1319
BstMAI GTCTC 6 cut(s) 68, 338, 764, 868, 1013, 1071
BstMBI GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 4 cut(s) 407, 440, 675, 1337
BstNI CCWGG 1 cut(s) 1254
BstSCI CCNGG 1 cut(s) 1252
BstSFI CTRYAG 2 cut(s) 134, 1455
BstUI CGCG 1 cut(s) 44
BstV1I GCAGC 4 cut(s) 9, 31, 839, 955
BstX2I RGATCY 1 cut(s) 448
BstYI RGATCY 1 cut(s) 448
BstZ17I GTATAC 1 cut(s) 227
BsuRI GGCC 1 cut(s) 846
BtgI CCRYGG 2 cut(s) 185, 290
BtsIMutI CAGTG 3 cut(s) 812, 906, 1268
CfoI GCGC 1 cut(s) 1279
Cfr13I GGNCC 1 cut(s) 844
CseI GACGC 3 cut(s) 28, 259, 974
Csp6I GTAC 3 cut(s) 115, 359, 563
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviAII CATG 9 cut(s) 186, 291, 465, 588, 885, 931, 1012, 1216, 1324
CviQI GTAC 3 cut(s) 115, 359, 563
DdeI CTNAG 6 cut(s) 78, 156, 411, 633, 768, 1390
DpnI GATC 8 cut(s) 28, 91, 373, 450, 866, 1180, 1229, 1318
DpnII GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
Eam1104I CTCTTC 2 cut(s) 654, 1178
EarI CTCTTC 2 cut(s) 654, 1178
Eco130I CCWWGG 4 cut(s) 185, 290, 976, 1300
Eco31I GGTCTC 2 cut(s) 868, 1013
Eco32I GATATC 1 cut(s) 267
Eco57I CTGAAG 1 cut(s) 437
Eco88I CYCGRG 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 1252
EcoRV GATATC 1 cut(s) 267
EcoT14I CCWWGG 4 cut(s) 185, 290, 976, 1300
ErhI CCWWGG 4 cut(s) 185, 290, 976, 1300
FaeI CATG 9 cut(s) 189, 294, 468, 591, 888, 934, 1015, 1219, 1327
FatI CATG 9 cut(s) 185, 290, 464, 587, 884, 930, 1011, 1215, 1323
FblI GTMKAC 1 cut(s) 226
Fnu4HI GCNGC 4 cut(s) 20, 23, 828, 969
Fsp4HI GCNGC 4 cut(s) 20, 23, 828, 969
FspBI CTAG 7 cut(s) 308, 368, 977, 1109, 1121, 1187, 1301
GlaI GCGC 1 cut(s) 1278
GluI GCNGC 4 cut(s) 20, 23, 828, 969
GsuI CTGGAG 1 cut(s) 465
HaeIII GGCC 1 cut(s) 846
HgaI GACGC 3 cut(s) 28, 259, 974
HhaI GCGC 1 cut(s) 1279
Hin1I GRCGYC 2 cut(s) 39, 251
Hin1II CATG 9 cut(s) 189, 294, 468, 591, 888, 934, 1015, 1219, 1327
Hin6I GCGC 1 cut(s) 1277
HinP1I GCGC 1 cut(s) 1277
HindIII AAGCTT 1 cut(s) 667
HinfI GANTC 6 cut(s) 210, 311, 644, 752, 1054, 1441
HphI GGTGA 3 cut(s) 135, 274, 1202
Hpy166II GTNNAC 3 cut(s) 102, 227, 1250
Hpy188I TCNGA 5 cut(s) 220, 342, 769, 1156, 1178
Hpy188III TCNNGA 7 cut(s) 308, 321, 352, 692, 1128, 1225, 1438
Hpy8I GTNNAC 3 cut(s) 102, 227, 1250
Hpy99I CGWCG 2 cut(s) 44, 602
HpyAV CCTTC 3 cut(s) 714, 718, 1256
HpyCH4III ACNGT 9 cut(s) 138, 274, 358, 562, 807, 910, 928, 1272, 1312
HpyCH4V TGCA 5 cut(s) 473, 899, 958, 1045, 1410
HpyF10VI GCNNNNNNNGC 4 cut(s) 407, 440, 675, 1337
HpyF3I CTNAG 6 cut(s) 78, 156, 411, 633, 768, 1390
Hsp92I GRCGYC 2 cut(s) 39, 251
Hsp92II CATG 9 cut(s) 189, 294, 468, 591, 888, 934, 1015, 1219, 1327
HspAI GCGC 1 cut(s) 1277
Kzo9I GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
LguI GCTCTTC 1 cut(s) 1178
LmnI GCTCC 2 cut(s) 189, 1328
Lsp1109I GCAGC 4 cut(s) 9, 31, 839, 955
LweI GCATC 2 cut(s) 388, 1183
MaeI CTAG 7 cut(s) 308, 368, 977, 1109, 1121, 1187, 1301
MaeIII GTNAC 3 cut(s) 573, 816, 1096
MalI GATC 8 cut(s) 28, 91, 373, 450, 866, 1180, 1229, 1318
MboI GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
MboII GAAGA 8 cut(s) 443, 544, 614, 671, 729, 745, 790, 1165
MfeI CAATTG 1 cut(s) 231
MflI RGATCY 1 cut(s) 448
MhlI GDGCHC 3 cut(s) 279, 595, 1173
MluCI AATT 4 cut(s) 231, 578, 959, 1211
MlyI GAGTC 1 cut(s) 1048
MmeI TCCRAC 3 cut(s) 621, 780, 1294
MnlI CCTC 7 cut(s) 230, 240, 328, 406, 503, 560, 943
MroXI GAANNNNTTC 1 cut(s) 214
MseI TTAA 1 cut(s) 855
MslI CAYNNNNRTG 1 cut(s) 1400
MspA1I CMGCKG 2 cut(s) 22, 107
MspR9I CCNGG 1 cut(s) 1254
MunI CAATTG 1 cut(s) 231
MvaI CCWGG 1 cut(s) 1254
MvnI CGCG 1 cut(s) 44
MwoI GCNNNNNNNGC 4 cut(s) 407, 440, 675, 1337
NcoI CCATGG 2 cut(s) 185, 290
NdeII GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
NlaIII CATG 9 cut(s) 189, 294, 468, 591, 888, 934, 1015, 1219, 1327
NlaIV GGNNCC 2 cut(s) 812, 1021
NmuCI GTSAC 1 cut(s) 816
PaeR7I CTCGAG 1 cut(s) 319
PciSI GCTCTTC 1 cut(s) 1178
PcsI WCGNNNNNNNCGW 1 cut(s) 1364
PdmI GAANNNNTTC 1 cut(s) 214
PfeI GAWTC 5 cut(s) 210, 311, 644, 752, 1441
PflMI CCANNNNNTGG 1 cut(s) 206
PkrI GCNGC 4 cut(s) 21, 24, 829, 970
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 1 cut(s) 1048
PpsI GAGTC 1 cut(s) 1048
Psp6I CCWGG 1 cut(s) 1252
PspGI CCWGG 1 cut(s) 1252
PspN4I GGNNCC 2 cut(s) 812, 1021
PspPI GGNCC 1 cut(s) 844
PsuI RGATCY 1 cut(s) 448
PvuI CGATCG 1 cut(s) 29
PvuII CAGCTG 2 cut(s) 22, 107
RsaI GTAC 3 cut(s) 116, 360, 564
RsaNI GTAC 3 cut(s) 115, 359, 563
RseI CAYNNNNRTG 1 cut(s) 1400
SapI GCTCTTC 1 cut(s) 1178
SaqAI TTAA 1 cut(s) 855
SatI GCNGC 4 cut(s) 20, 23, 828, 969
Sau3AI GATC 8 cut(s) 26, 89, 371, 448, 864, 1178, 1227, 1316
Sau96I GGNCC 1 cut(s) 844
SchI GAGTC 1 cut(s) 1048
ScrFI CCNGG 1 cut(s) 1254
SduI GDGCHC 3 cut(s) 279, 595, 1173
SfaNI GCATC 2 cut(s) 388, 1183
SfcI CTRYAG 2 cut(s) 134, 1455
Sfr274I CTCGAG 1 cut(s) 319
SlaI CTCGAG 1 cut(s) 319
SmiMI CAYNNNNRTG 1 cut(s) 1400
SmlI CTYRAG 1 cut(s) 319
SmoI CTYRAG 1 cut(s) 319
Sse9I AATT 4 cut(s) 231, 578, 959, 1211
SsiI CCGC 1 cut(s) 495
SspI AATATT 1 cut(s) 546
SspMI CTAG 7 cut(s) 308, 368, 977, 1109, 1121, 1187, 1301
StyD4I CCNGG 1 cut(s) 1252
StyI CCWWGG 4 cut(s) 185, 290, 976, 1300
TaaI ACNGT 9 cut(s) 138, 274, 358, 562, 807, 910, 928, 1272, 1312
TaqI TCGA 5 cut(s) 269, 320, 600, 788, 1289
TasI AATT 4 cut(s) 231, 578, 959, 1211
TatI WGTACW 1 cut(s) 358
TfiI GAWTC 5 cut(s) 210, 311, 644, 752, 1441
Tru1I TTAA 1 cut(s) 855
Tru9I TTAA 1 cut(s) 855
TscAI CASTG 3 cut(s) 812, 913, 1275
TseFI GTSAC 1 cut(s) 816
TseI GCWGC 4 cut(s) 19, 22, 827, 968
Tsp45I GTSAC 1 cut(s) 816
TspDTI ATGAA 4 cut(s) 636, 883, 1204, 1312
TspGWI ACGGA 2 cut(s) 774, 1161
TspRI CASTG 3 cut(s) 812, 913, 1275
Van91I CCANNNNNTGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 1211
XbaI TCTAGA 1 cut(s) 307
XcmI CCANNNNNNNNNTGG 1 cut(s) 838
XhoI CTCGAG 1 cut(s) 319
XmaJI CCTAGG 2 cut(s) 976, 1300
XmiI GTMKAC 1 cut(s) 226
XmnI GAANNNNTTC 1 cut(s) 214
XspI CTAG 7 cut(s) 308, 368, 977, 1109, 1121, 1187, 1301
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.