Prupe.4G210100_v2.0.a1

BT1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0013256
Physical Location & Seq
Forward (+)
13103238 .. 13107194
3957 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G210100.1

Sequence Viewer

Length: 1494 bp
ATGATACAATGGCTGAAAAGATTAAGAGCTGCATTTGGAGCCTCGTTTCTCTGGCTCGTTTGCTTGGTTTACTTCACCCAGGGTTTCAGATCCTTTGTATGGACGGCAGTTTCTTACCAGCTGAAAGATAGGCTTAAGTTGTCACCCTCATCTTCCCAATTTGTGTTTTCAATTGCTTTCTTCCCATGGAGCATTAAACCATTATATGGAATTGTGTCTGATTGTATTCCAATAAAAGGAAGAAAAAGGGTTCCGTATTTAGTGATTGCAACTGTGCTCTCTCTGGTGCCGTGGCTTATCCTTGGCCTGAATGCAACGTTGAGGAGTTCCAAATGGAACCTCATGACCTTCTTAACAGTCCAGAACCTGGGCTCAGCCATGGCAGATGTGGTAGTTGATGCAATGATTGCTGAGGCAGTAAGACGTGAGCGGGCCACATTTGCTGGAGACCTCCAGTCGATATCATGGTTTTCAATGGCTTTGGGCGGCATATGCGGGAGTTTGTCAGGAGGATATGCACTAACCAACTTACAGATACATACGATTTTTCTTCTTTTTTCTGTACTACCATCCATACAGTTGTTATCATGTGGCTTGGTTGAGGAGAAATCTGTTACTGTAGATAGTAAAGTTTTGCCCGAATTTTATGACTCAAGTAGTGTCGATGGGGTGAATGGAAATAGTAGTTTTCTAGATGAAGATAGTTTCTCAGAGAAGAAACCCAATATCAGCATGTTAAGGAGAAAGAAAAGTCGTAAGGGCGGCAAAAAGAAACGAGGTACTGGTAAAGGCCAGATTGCTGAAAAAGTTGACTCGTTGCCTTCATGGTGGTTCCGCTCTCTCAAAGCAGCCACTTTTAGTTTGTGCCATGCATTTAAACAACCACTCATTTTGAGACCAATGGCTTGGTTTTTCCTAGCACATGTTTCCATTCCAAACCTCTCGACTGTGATGTTTTATTATCAGACAGAGTTTTTGAGCTTGGAAGCCTCCTTTTTGGGCACTGCACGTGTTGTGGGATGGTTAGGCCTCATGCTTGGAACCTTTGTATATAATCAGTACTTAAAGACAATGAAATTAAGAAGGATCCTCATGTTGTCTCATGTTGGTTTGTCTTTCTTGGGTCTCCTAGATATGGTTTTAGTCTCTCGAGTAAATCTCACCTACGGAATATCAGATAGAATTATGGTGCTTTGTGGCTCAGCTCTCAGTGATGCAATCAATCAATTCAAGTTTATGCCATTTCTGATCTTATCTGGACAGCTATGCCCACCTGGAATTGAAGGGACTCTGTTCGCCCTCTTTATGTCGATAAACAACTTAGGGTCAACATTGGGGTCATTTGTCGGTGCTGGGTTGGCTTCAGCTTTAAACATCTCTTCAGGTTCTTTTGACAACCTTTCTCTGGGAATTGCCATTCAAGTTCTCTGCACTTTCATTCCTGTTGGTTTTCTATTTTTGATACCAAAGGAAGTTACAGGGGTATCGGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

498

Amino Acids

54.79

Weight (kDa)

9.66

Isoelectric Point (pI)

40.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54860
fragaria_vesca FvH4_3g22370 FvH4_3g22370 FvH4_3g22370 FvH4_3g22370
malus_domestica MD03G1205600.v1.1
prunus_persica Prupe.4G210100_v2.0.a1
pyrus_communis pycom03g15530
rosa_chinensis RchiOBHm_Chr5g0039011
rosa_laevigata RLG00000033883
rosa_multiflora Rmu_sc0041879.1_g000001
rosa_roxburghii Rroxscaffold_1G00042110
rosa_rugosa Rorug05G0175600
rosa_samantha Rh5AG262300 Rh5BG266900 Rh5CG299500 Rh5DG273400
rosa_wichuraiana Rw5G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 286
AccB7I CCANNNNNTGG 2 cut(s) 206, 367
AccBSI CCGCTC 2 cut(s) 430, 837
AciI CCGC 5 cut(s) 430, 486, 495, 762, 835
AclI AACGTT 1 cut(s) 317
AclWI GGATC 3 cut(s) 84, 1081, 1094
AcsI RAATTY 1 cut(s) 641
AcuI CTGAAG 2 cut(s) 1347, 1365
AcvI CACGTG 1 cut(s) 1010
AfaI GTAC 3 cut(s) 564, 781, 1061
AfiI CCNNNNNNNGG 6 cut(s) 99, 206, 236, 367, 1135, 1405
AflII CTTAAG 1 cut(s) 134
AflIII ACRYGT 2 cut(s) 922, 1009
AgsI TTSAA 5 cut(s) 171, 474, 1231, 1283, 1421
AhdI GACNNNNNGTC 1 cut(s) 454
AjiI CACGTC 1 cut(s) 425
AjnI CCWGG 3 cut(s) 78, 366, 1273
AluBI AGCT 6 cut(s) 29, 121, 981, 1205, 1264, 1367
AluI AGCT 6 cut(s) 29, 121, 981, 1205, 1264, 1367
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 5 cut(s) 441, 889, 1104, 1130, 1150
AlwI GGATC 3 cut(s) 84, 1081, 1094
AlwNI CAGNNNCTG 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 1149
AoxI GGCC 4 cut(s) 304, 432, 790, 1027
ApeKI GCWGC 2 cut(s) 29, 848
ApoI RAATTY 1 cut(s) 641
ArsI GACNNNNNNTTYG 2 cut(s) 958, 990
AspS9I GGNCC 1 cut(s) 432
AsuHPI GGTGA 4 cut(s) 67, 135, 682, 1153
AvaI CYCGRG 1 cut(s) 1149
BaeGI GKGCMC 1 cut(s) 1004
BaeI ACNNNNGTAYC 1 cut(s) 1467
BamHI GGATCC 1 cut(s) 1086
BanI GGYRCC 1 cut(s) 286
BanII GRGCYC 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 1010
Bbv12I GWGCWC 1 cut(s) 279
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 2 cut(s) 16, 860
BccI CCATC 3 cut(s) 577, 659, 1014
BceAI ACGGC 2 cut(s) 120, 274
BciT130I CCWGG 3 cut(s) 80, 368, 1275
BcoDI GTCTC 5 cut(s) 441, 889, 1104, 1130, 1150
BfaI CTAG 3 cut(s) 692, 917, 1130
BfmI CTRYAG 1 cut(s) 618
BfrI CTTAAG 1 cut(s) 134
BisI GCNGC 4 cut(s) 30, 487, 763, 849
BlpI GCTNAGC 2 cut(s) 373, 1201
BlsI GCNGC 4 cut(s) 31, 488, 764, 850
BmcAI AGTACT 1 cut(s) 1061
Bme1390I CCNGG 3 cut(s) 80, 368, 1275
BmeRI GACNNNNNGTC 1 cut(s) 454
BmeT110I CYCGRG 1 cut(s) 1149
BmgBI CACGTC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 432
BmiI GGNNCC 7 cut(s) 40, 252, 288, 338, 833, 1042, 1088
BmrFI CCNGG 3 cut(s) 80, 368, 1275
BmsI GCATC 2 cut(s) 388, 1204
BplI GAGNNNNNCTC 2 cut(s) 1143, 1175
BpmI CTGGAG 2 cut(s) 437, 465
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 2 cut(s) 373, 1201
BpuEI CTTGAG 1 cut(s) 637
BsaAI YACGTR 1 cut(s) 1010
BsaI GGTCTC 3 cut(s) 441, 889, 1130
BsaJI CCNNGG 7 cut(s) 78, 79, 185, 290, 301, 367, 378
Bsc4I CCNNNNNNNGG 6 cut(s) 99, 206, 236, 367, 1135, 1405
Bse1I ACTGG 2 cut(s) 454, 787
Bse3DI GCAATG 1 cut(s) 408
BseBI CCWGG 3 cut(s) 80, 368, 1275
BseDI CCNNGG 7 cut(s) 78, 79, 185, 290, 301, 367, 378
BseGI GGATG 2 cut(s) 569, 1025
BseLI CCNNNNNNNGG 6 cut(s) 99, 206, 236, 367, 1135, 1405
BseMI GCAATG 1 cut(s) 408
BseMII CTCAG 5 cut(s) 387, 402, 723, 1215, 1222
BseNI ACTGG 2 cut(s) 454, 787
BseRI GAGGAG 2 cut(s) 337, 617
BseSI GKGCMC 1 cut(s) 1004
BseXI GCAGC 2 cut(s) 16, 860
BseYI CCCAGC 1 cut(s) 1352
BsgI GTGCAG 2 cut(s) 990, 1414
BshFI GGCC 4 cut(s) 306, 434, 792, 1029
BshNI GGYRCC 1 cut(s) 286
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 1149
BslFI GGGAC 1 cut(s) 1300
BslI CCNNNNNNNGG 6 cut(s) 99, 206, 236, 367, 1135, 1405
BsmAI GTCTC 5 cut(s) 441, 889, 1104, 1130, 1150
BsmFI GGGAC 1 cut(s) 1300
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 4 cut(s) 306, 434, 792, 1029
Bso31I GGTCTC 3 cut(s) 441, 889, 1130
BsoBI CYCGRG 1 cut(s) 1149
Bsp1286I GDGCHC 3 cut(s) 279, 374, 1004
Bsp143I GATC 3 cut(s) 89, 1086, 1248
Bsp1720I GCTNAGC 2 cut(s) 373, 1201
Bsp19I CCATGG 2 cut(s) 185, 378
BspACI CCGC 5 cut(s) 430, 486, 495, 762, 835
BspANI GGCC 4 cut(s) 306, 434, 792, 1029
BspCNI CTCAG 5 cut(s) 386, 403, 722, 1214, 1221
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 7 cut(s) 40, 252, 288, 338, 833, 1042, 1088
BspPI GGATC 3 cut(s) 84, 1081, 1094
BspT107I GGYRCC 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 134
BspTNI GGTCTC 3 cut(s) 441, 889, 1130
BsrBI CCGCTC 2 cut(s) 430, 837
BsrDI GCAATG 1 cut(s) 408
BsrI ACTGG 2 cut(s) 454, 787
BssECI CCNNGG 7 cut(s) 78, 79, 185, 290, 301, 367, 378
BssMI GATC 3 cut(s) 89, 1086, 1248
BssT1I CCWWGG 3 cut(s) 185, 301, 378
Bst2UI CCWGG 3 cut(s) 80, 368, 1275
Bst4CI ACNGT 5 cut(s) 274, 358, 579, 619, 949
Bst6I CTCTTC 1 cut(s) 1384
BstAFI CTTAAG 1 cut(s) 134
BstAPI GCANNNNNTGC 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 1010
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 6 cut(s) 373, 411, 709, 1201, 1208, 1321
BstDSI CCRYGG 3 cut(s) 185, 290, 378
BstF5I GGATG 2 cut(s) 569, 1025
BstKTI GATC 3 cut(s) 92, 1089, 1251
BstMAI GTCTC 5 cut(s) 441, 889, 1104, 1130, 1150
BstMBI GATC 3 cut(s) 89, 1086, 1248
BstMWI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 492, 1358
BstNI CCWGG 3 cut(s) 80, 368, 1275
BstNSI RCATGY 2 cut(s) 736, 926
BstSCI CCNGG 3 cut(s) 78, 366, 1273
BstSFI CTRYAG 1 cut(s) 618
BstSLI GKGCMC 1 cut(s) 1004
BstV1I GCAGC 2 cut(s) 16, 860
BstX2I RGATCY 2 cut(s) 89, 1086
BstXI CCANNNNNNTGG 1 cut(s) 906
BstYI RGATCY 2 cut(s) 89, 1086
BsuRI GGCC 4 cut(s) 306, 434, 792, 1029
BtgI CCRYGG 3 cut(s) 185, 290, 378
BtrI CACGTC 1 cut(s) 425
BtsCI GGATG 2 cut(s) 569, 1025
BtsI GCAGTG 1 cut(s) 1002
BtsIMutI CAGTG 2 cut(s) 1002, 1216
Cac8I GCNNGC 1 cut(s) 432
CaiI CAGNNNCTG 1 cut(s) 367
CciI TCATGA 1 cut(s) 342
Cfr13I GGNCC 1 cut(s) 432
Csp6I GTAC 3 cut(s) 563, 780, 1060
CspCI CAANNNNNGTGG 2 cut(s) 873, 908
CviQI GTAC 3 cut(s) 563, 780, 1060
DdeI CTNAG 6 cut(s) 373, 411, 709, 1201, 1208, 1321
DpnI GATC 3 cut(s) 91, 1088, 1250
DpnII GATC 3 cut(s) 89, 1086, 1248
DraI TTTAAA 2 cut(s) 877, 1371
DriI GACNNNNNGTC 1 cut(s) 454
Eam1104I CTCTTC 1 cut(s) 1384
Eam1105I GACNNNNNGTC 1 cut(s) 454
EarI CTCTTC 1 cut(s) 1384
Eco130I CCWWGG 3 cut(s) 185, 301, 378
Eco147I AGGCCT 1 cut(s) 1029
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 3 cut(s) 441, 889, 1130
Eco32I GATATC 1 cut(s) 462
Eco57I CTGAAG 2 cut(s) 1347, 1365
Eco72I CACGTG 1 cut(s) 1010
Eco88I CYCGRG 1 cut(s) 1149
EcoRII CCWGG 3 cut(s) 78, 366, 1273
EcoRV GATATC 1 cut(s) 462
EcoT14I CCWWGG 3 cut(s) 185, 301, 378
EcoT22I ATGCAT 1 cut(s) 874
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 3 cut(s) 185, 301, 378
FalI AAGNNNNNCTT 2 cut(s) 117, 149
FaqI GGGAC 1 cut(s) 1300
FauI CCCGC 2 cut(s) 423, 488
FauNDI CATATG 1 cut(s) 491
Fnu4HI GCNGC 4 cut(s) 30, 487, 763, 849
FokI GGATG 2 cut(s) 556, 1032
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 30, 487, 763, 849
FspBI CTAG 3 cut(s) 692, 917, 1130
GluI GCNGC 4 cut(s) 30, 487, 763, 849
GsaI CCCAGC 1 cut(s) 1356
GsuI CTGGAG 2 cut(s) 437, 465
HaeIII GGCC 4 cut(s) 306, 434, 792, 1029
HincII GTYRAC 2 cut(s) 811, 1329
HindII GTYRAC 2 cut(s) 811, 1329
HinfI GANTC 3 cut(s) 650, 812, 1288
HphI GGTGA 4 cut(s) 67, 135, 682, 1153
Hpy166II GTNNAC 3 cut(s) 70, 811, 1329
Hpy188I TCNGA 6 cut(s) 89, 220, 712, 966, 1177, 1248
Hpy188III TCNNGA 7 cut(s) 343, 361, 507, 692, 943, 1149, 1257
Hpy8I GTNNAC 3 cut(s) 70, 811, 1329
HpyAV CCTTC 4 cut(s) 358, 831, 1077, 1277
HpyCH4III ACNGT 5 cut(s) 274, 358, 579, 619, 949
HpyCH4IV ACGT 3 cut(s) 317, 424, 1009
HpyCH4V TGCA 9 cut(s) 32, 269, 314, 401, 518, 872, 1007, 1217, 1431
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 492, 1358
HpyF3I CTNAG 6 cut(s) 373, 411, 709, 1201, 1208, 1321
HpySE526I ACGT 3 cut(s) 317, 424, 1009
Kzo9I GATC 3 cut(s) 89, 1086, 1248
LmnI GCTCC 2 cut(s) 38, 189
Lsp1109I GCAGC 2 cut(s) 16, 860
LweI GCATC 2 cut(s) 388, 1204
MaeI CTAG 3 cut(s) 692, 917, 1130
MaeII ACGT 3 cut(s) 317, 424, 1009
MaeIII GTNAC 3 cut(s) 141, 613, 1474
MalI GATC 3 cut(s) 91, 1088, 1250
MbiI CCGCTC 2 cut(s) 430, 837
MboI GATC 3 cut(s) 89, 1086, 1248
MboII GAAGA 7 cut(s) 144, 172, 252, 542, 710, 727, 1371
MfeI CAATTG 1 cut(s) 171
MflI RGATCY 2 cut(s) 89, 1086
MhlI GDGCHC 3 cut(s) 279, 374, 1004
MluCI AATT 9 cut(s) 158, 171, 210, 641, 1076, 1182, 1226, 1278, 1410
MlyI GAGTC 3 cut(s) 644, 806, 1282
Mph1103I ATGCAT 1 cut(s) 874
MseI TTAA 9 cut(s) 23, 135, 195, 353, 737, 876, 1064, 1079, 1370
MspA1I CMGCKG 1 cut(s) 121
MspCI CTTAAG 1 cut(s) 134
MspR9I CCNGG 3 cut(s) 80, 368, 1275
MunI CAATTG 1 cut(s) 171
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 3 cut(s) 80, 368, 1275
MwoI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 492, 1358
NcoI CCATGG 2 cut(s) 185, 378
NdeI CATATG 1 cut(s) 491
NdeII GATC 3 cut(s) 89, 1086, 1248
NlaIV GGNNCC 7 cut(s) 40, 252, 288, 338, 833, 1042, 1088
NmuCI GTSAC 1 cut(s) 141
NsiI ATGCAT 1 cut(s) 874
NspI RCATGY 2 cut(s) 736, 926
PaeR7I CTCGAG 1 cut(s) 1149
PagI TCATGA 1 cut(s) 342
PasI CCCWGGG 1 cut(s) 79
PceI AGGCCT 1 cut(s) 1029
PciI ACATGT 1 cut(s) 922
PctI GAATGC 1 cut(s) 316
PflMI CCANNNNNTGG 2 cut(s) 206, 367
PkrI GCNGC 4 cut(s) 31, 488, 764, 850
PleI GAGTC 3 cut(s) 644, 806, 1282
PmaCI CACGTG 1 cut(s) 1010
PmlI CACGTG 1 cut(s) 1010
PpsI GAGTC 3 cut(s) 644, 806, 1282
Ppu21I YACGTR 1 cut(s) 1010
PscI ACATGT 1 cut(s) 922
Psp1406I AACGTT 1 cut(s) 317
Psp6I CCWGG 3 cut(s) 78, 366, 1273
PspCI CACGTG 1 cut(s) 1010
PspFI CCCAGC 1 cut(s) 1352
PspGI CCWGG 3 cut(s) 78, 366, 1273
PspN4I GGNNCC 7 cut(s) 40, 252, 288, 338, 833, 1042, 1088
PspPI GGNCC 1 cut(s) 432
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 89, 1086
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 3 cut(s) 564, 781, 1061
RsaNI GTAC 3 cut(s) 563, 780, 1060
SaqAI TTAA 9 cut(s) 23, 135, 195, 353, 737, 876, 1064, 1079, 1370
SatI GCNGC 4 cut(s) 30, 487, 763, 849
Sau3AI GATC 3 cut(s) 89, 1086, 1248
Sau96I GGNCC 1 cut(s) 432
ScaI AGTACT 1 cut(s) 1061
SchI GAGTC 3 cut(s) 644, 806, 1282
ScrFI CCNGG 3 cut(s) 80, 368, 1275
SduI GDGCHC 3 cut(s) 279, 374, 1004
SfaNI GCATC 2 cut(s) 388, 1204
SfcI CTRYAG 1 cut(s) 618
Sfr274I CTCGAG 1 cut(s) 1149
SlaI CTCGAG 1 cut(s) 1149
SmlI CTYRAG 3 cut(s) 134, 652, 1149
SmoI CTYRAG 3 cut(s) 134, 652, 1149
Sse9I AATT 9 cut(s) 158, 171, 210, 641, 1076, 1182, 1226, 1278, 1410
SseBI AGGCCT 1 cut(s) 1029
SsiI CCGC 5 cut(s) 430, 486, 495, 762, 835
SspMI CTAG 3 cut(s) 692, 917, 1130
StuI AGGCCT 1 cut(s) 1029
StyD4I CCNGG 3 cut(s) 78, 366, 1273
StyI CCWWGG 3 cut(s) 185, 301, 378
TaaI ACNGT 5 cut(s) 274, 358, 579, 619, 949
TaiI ACGT 3 cut(s) 320, 427, 1012
TaqI TCGA 5 cut(s) 458, 663, 944, 1150, 1310
TasI AATT 9 cut(s) 158, 171, 210, 641, 1076, 1182, 1226, 1278, 1410
TatI WGTACW 2 cut(s) 562, 1059
TauI GCSGC 2 cut(s) 489, 765
Tru1I TTAA 9 cut(s) 23, 135, 195, 353, 737, 876, 1064, 1079, 1370
Tru9I TTAA 9 cut(s) 23, 135, 195, 353, 737, 876, 1064, 1079, 1370
TscAI CASTG 2 cut(s) 1009, 1216
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 2 cut(s) 29, 848
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 711, 813, 1088, 1426
TspGWI ACGGA 2 cut(s) 243, 1182
TspRI CASTG 2 cut(s) 1009, 1216
Van91I CCANNNNNTGG 2 cut(s) 206, 367
Vha464I CTTAAG 1 cut(s) 134
XapI RAATTY 1 cut(s) 641
XbaI TCTAGA 1 cut(s) 691
XceI RCATGY 2 cut(s) 736, 926
XcmI CCANNNNNNNNNTGG 1 cut(s) 385
XhoI CTCGAG 1 cut(s) 1149
XspI CTAG 3 cut(s) 692, 917, 1130
ZrmI AGTACT 1 cut(s) 1061
Zsp2I ATGCAT 1 cut(s) 874
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.