Rroxscaffold_1G00042110

BT1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0013256
Physical Location & Seq
Reverse (-)
60138602 .. 60142610
4009 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00042110.1

Sequence Viewer

Length: 1488 bp
ATGATTGAATGGCTGAAGAGACTAAGAGCTGCATTTGGAGCCTCGTTTCTCTGGCTTGTTTGCCTCATTTACTTCACCCAGGGTTTTAGATCCTTTGTTTGGACTGCAGTTTCTTACCAGCTGAAAGATACTCTGAAGTTGTCACCTTCGTCTTCCCAGTTTGTGTTTTCGATAGCCTTCTTCCCATGGAGCATTAAACCATTATATGGAATTTTGTCTGATTGTATTCCAATAAAAGGAAGGAAAAGGGTTCCATACTTAGTTATTGCAACTGTGCTCTCTCTTGTCCCATGGCCTATTCTCGGACTGAATGCAACGTCAAGGAGTTCCACGTGGCTCCTCATGACCTTCTTAACAGTCCAGAACCTGGGCTCGGCCATGGCTGATGTAGTAGTTGATGCGATGATTGCTGAGGCAGTAAGACATGAGCGGGCTTCATTTGCTGGAGATCTCCAGTCAATATCGTGGTTGGCAATGGCTTTCGGCGGCATATGTGGGAGTTTGACAGGAGGATTTGCATTAACCAACTTTCATATACACACAATTTTTCTTCTTTTTTCTGTACTACCATCCATACAATTGCTATCATGTGGCTTGGTTGAGGAGAATTCTGTAGATAGTAAAGCTTTCCCTGAATTTTCCAAGTTAAAAAGCTACGATGGGGTGAATGGGAACGGTAGTTCTCTGGATGAAGATAGTTTTTCAGAGAAGAAATCCAATATCAGCATTACAAGGAGAAAGAAGAGCAATAAGGGCAGCAAAAAGAAAACAGTTTCTAATAAAGTCCAGGTTTCTGGGAAAGTTGATTCACTAGCATCACAGTGGTTCCAATCTCTGAAAGCAGCCTCTTTTAGTCTATGCCGTGCATTTAAACAACCAATTATTTTGAGACCAATGGCTTGGTTTTTCCTGGCACATGTTTCCATTCCAAACCTCTCGACTGTGATGTTTTATTATCAAACAGAGTTCTTGAACTTAGAAGCATCCTTTTTGGGCACTGCACGTGTTGTGGGATGGTTAGGCCTCATGCTTGGAACGTTCGTATATAACAAGTACTTAAAGAGAATGAAGTTGCGAAGAATCCTCATGTGGTCTCAAGTTGGTTTGGCTTTCTTGACTCTCCTGGACATTGTTTTAGTGTCTCGAGTAAATCTCACCTTTGGAATATCAGATAGGATTATGGTGCTCTGTGGCTCAGCTCTTAGTGATGCAGTCAATCAGTTCAAGTTTATGCCATTCCTGATCTTATCTGGACAACTTTGCCCTCCTGGAATTGAGGGCACTCTCTTTGCCCTTTTTATGTCGATCAATAATTTAGGGTCAACGTTGGGGTCATTTGTCGGTGCTGGGTTGGCTTCAGTTTTGAGCATCTCTTCAGGTTCTTTTGACAACCTTCTATTGGGAATTGTCATTCAAGTTCTCTGCATATTCATTCCAATTGGTTTCCTATTTTTGATACCGAAGGAAGCTACAGGAATATCCGCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

495

Amino Acids

54.49

Weight (kDa)

9.63

Isoelectric Point (pI)

36.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 25 - 444 4.1e-21 Major Facilitator Superfamily
BT1 PF03092 27 - 488 8.5e-104 BT1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54860
fragaria_vesca FvH4_3g22370 FvH4_3g22370 FvH4_3g22370 FvH4_3g22370
malus_domestica MD03G1205600.v1.1
prunus_persica Prupe.4G210100_v2.0.a1
pyrus_communis pycom03g15530
rosa_chinensis RchiOBHm_Chr5g0039011
rosa_laevigata RLG00000033883
rosa_multiflora Rmu_sc0041879.1_g000001
rosa_roxburghii Rroxscaffold_1G00042110
rosa_rugosa Rorug05G0175600
rosa_samantha Rh5AG262300 Rh5BG266900 Rh5CG299500 Rh5DG273400
rosa_wichuraiana Rw5G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 206, 367
AccBSI CCGCTC 1 cut(s) 430
AciI CCGC 3 cut(s) 430, 486, 1482
AclI AACGTT 2 cut(s) 1037, 1325
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 3 cut(s) 210, 607, 635
AcuI CTGAAG 4 cut(s) 35, 155, 1341, 1359
AcvI CACGTG 2 cut(s) 333, 1004
AfaI GTAC 2 cut(s) 564, 1055
AfiI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1399
AflIII ACRYGT 2 cut(s) 916, 1003
AgsI TTSAA 4 cut(s) 8, 973, 1225, 1415
AjnI CCWGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
AloI GAACNNNNNNTCC 2 cut(s) 664, 696
AluBI AGCT 6 cut(s) 29, 121, 626, 654, 1199, 1469
AluI AGCT 6 cut(s) 29, 121, 626, 654, 1199, 1469
Alw21I GWGCWC 2 cut(s) 279, 1188
Alw26I GTCTC 4 cut(s) 13, 883, 1098, 1146
AlwI GGATC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 1143
AoxI GGCC 3 cut(s) 293, 375, 1021
ApeKI GCWGC 3 cut(s) 29, 756, 842
ApoI RAATTY 3 cut(s) 210, 607, 635
AsuHPI GGTGA 4 cut(s) 67, 135, 676, 1147
AvaI CYCGRG 1 cut(s) 1143
BaeGI GKGCMC 2 cut(s) 998, 1283
BanII GRGCYC 1 cut(s) 374
BbrPI CACGTG 2 cut(s) 333, 1004
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 2 cut(s) 279, 1188
BbvCI CCTCAGC 1 cut(s) 411
BbvI GCAGC 3 cut(s) 16, 768, 854
BccI CCATC 3 cut(s) 577, 653, 1008
BceAI ACGGC 1 cut(s) 846
BciT130I CCWGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
BcoDI GTCTC 4 cut(s) 13, 883, 1098, 1146
BfaI CTAG 1 cut(s) 812
BfmI CTRYAG 3 cut(s) 105, 612, 1470
BglII AGATCT 1 cut(s) 448
BisI GCNGC 4 cut(s) 30, 487, 757, 843
BlpI GCTNAGC 1 cut(s) 1195
BlsI GCNGC 4 cut(s) 31, 488, 758, 844
BmcAI AGTACT 1 cut(s) 1055
Bme1390I CCNGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
BmeT110I CYCGRG 1 cut(s) 1143
BmiI GGNNCC 4 cut(s) 40, 252, 338, 827
BmrFI CCNGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
BmrI ACTGGG 1 cut(s) 151
BmsI GCATC 5 cut(s) 388, 824, 992, 1198, 1377
BmuI ACTGGG 1 cut(s) 151
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 1137, 1169
BpmI CTGGAG 2 cut(s) 437, 465
Bpu10I CCTNAGC 1 cut(s) 411
Bpu1102I GCTNAGC 1 cut(s) 1195
BpuEI CTTGAG 1 cut(s) 1080
BsaAI YACGTR 2 cut(s) 333, 1004
BsaI GGTCTC 2 cut(s) 883, 1098
BsaJI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1399
Bse1I ACTGG 2 cut(s) 157, 454
Bse3DI GCAATG 1 cut(s) 480
BseBI CCWGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
BseDI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
BseGI GGATG 4 cut(s) 569, 694, 983, 1019
BseLI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1399
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 2 cut(s) 402, 1209
BseNI ACTGG 2 cut(s) 157, 454
BseRI GAGGAG 2 cut(s) 329, 617
BseSI GKGCMC 2 cut(s) 998, 1283
BseXI GCAGC 3 cut(s) 16, 768, 854
BseYI CCCAGC 1 cut(s) 1346
BsgI GTGCAG 1 cut(s) 984
BshFI GGCC 3 cut(s) 295, 377, 1023
BsiHKAI GWGCWC 2 cut(s) 279, 1188
BsiHKCI CYCGRG 1 cut(s) 1143
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1399
BsmAI GTCTC 4 cut(s) 13, 883, 1098, 1146
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 3 cut(s) 295, 377, 1023
Bso31I GGTCTC 2 cut(s) 883, 1098
BsoBI CYCGRG 1 cut(s) 1143
Bsp1286I GDGCHC 5 cut(s) 279, 374, 998, 1188, 1283
Bsp143I GATC 4 cut(s) 89, 448, 1242, 1305
Bsp1720I GCTNAGC 1 cut(s) 1195
Bsp19I CCATGG 3 cut(s) 185, 290, 378
BspACI CCGC 3 cut(s) 430, 486, 1482
BspANI GGCC 3 cut(s) 295, 377, 1023
BspCNI CTCAG 2 cut(s) 403, 1208
BspHI TCATGA 1 cut(s) 342
BspLI GGNNCC 4 cut(s) 40, 252, 338, 827
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 84
BspQI GCTCTTC 1 cut(s) 737
BspTNI GGTCTC 2 cut(s) 883, 1098
BsrBI CCGCTC 1 cut(s) 430
BsrDI GCAATG 1 cut(s) 480
BsrI ACTGG 2 cut(s) 157, 454
BssECI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
BssMI GATC 4 cut(s) 89, 448, 1242, 1305
BssT1I CCWWGG 3 cut(s) 185, 290, 378
Bst2UI CCWGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
Bst4CI ACNGT 6 cut(s) 274, 358, 677, 772, 822, 943
Bst6I CTCTTC 3 cut(s) 11, 737, 1378
BstBAI YACGTR 2 cut(s) 333, 1004
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 6 cut(s) 23, 259, 411, 976, 1195, 1202
BstDSI CCRYGG 3 cut(s) 185, 290, 378
BstF5I GGATG 4 cut(s) 569, 694, 983, 1019
BstKTI GATC 4 cut(s) 92, 451, 1245, 1308
BstMAI GTCTC 4 cut(s) 13, 883, 1098, 1146
BstMBI GATC 4 cut(s) 89, 448, 1242, 1305
BstMWI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 753, 1352
BstNI CCWGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
BstNSI RCATGY 1 cut(s) 920
BstSCI CCNGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
BstSFI CTRYAG 3 cut(s) 105, 612, 1470
BstSLI GKGCMC 2 cut(s) 998, 1283
BstV1I GCAGC 3 cut(s) 16, 768, 854
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 89, 448
BstXI CCANNNNNNTGG 2 cut(s) 794, 900
BstYI RGATCY 2 cut(s) 89, 448
BsuRI GGCC 3 cut(s) 295, 377, 1023
BtgI CCRYGG 3 cut(s) 185, 290, 378
BtgZI GCGATG 1 cut(s) 416
BtsCI GGATG 4 cut(s) 569, 694, 983, 1019
BtsI GCAGTG 1 cut(s) 996
BtsIMutI CAGTG 2 cut(s) 827, 996
Cac8I GCNNGC 1 cut(s) 432
CaiI CAGNNNCTG 1 cut(s) 367
CciI TCATGA 1 cut(s) 342
Csp6I GTAC 2 cut(s) 563, 1054
CviAII CATG 9 cut(s) 186, 291, 343, 379, 425, 588, 917, 1027, 1087
CviQI GTAC 2 cut(s) 563, 1054
DdeI CTNAG 6 cut(s) 23, 259, 411, 976, 1195, 1202
DpnI GATC 4 cut(s) 91, 450, 1244, 1307
DpnII GATC 4 cut(s) 89, 448, 1242, 1305
DraI TTTAAA 1 cut(s) 871
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 3 cut(s) 11, 737, 1378
EarI CTCTTC 3 cut(s) 11, 737, 1378
Eco130I CCWWGG 3 cut(s) 185, 290, 378
Eco147I AGGCCT 1 cut(s) 1023
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 2 cut(s) 883, 1098
Eco57I CTGAAG 4 cut(s) 35, 155, 1341, 1359
Eco72I CACGTG 2 cut(s) 333, 1004
Eco88I CYCGRG 1 cut(s) 1143
EcoRI GAATTC 1 cut(s) 607
EcoRII CCWGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
EcoT14I CCWWGG 3 cut(s) 185, 290, 378
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 3 cut(s) 185, 290, 378
FaeI CATG 9 cut(s) 189, 294, 346, 382, 428, 591, 920, 1030, 1090
FaqI GGGAC 1 cut(s) 272
FatI CATG 9 cut(s) 185, 290, 342, 378, 424, 587, 916, 1026, 1086
FauI CCCGC 1 cut(s) 423
FauNDI CATATG 1 cut(s) 491
Fnu4HI GCNGC 4 cut(s) 30, 487, 757, 843
FokI GGATG 4 cut(s) 556, 701, 970, 1026
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 30, 487, 757, 843
FspBI CTAG 1 cut(s) 812
GluI GCNGC 4 cut(s) 30, 487, 757, 843
GsaI CCCAGC 1 cut(s) 1350
GsuI CTGGAG 2 cut(s) 437, 465
HaeIII GGCC 3 cut(s) 295, 377, 1023
Hin1II CATG 9 cut(s) 189, 294, 346, 382, 428, 591, 920, 1030, 1090
HincII GTYRAC 1 cut(s) 1323
HindII GTYRAC 1 cut(s) 1323
HindIII AAGCTT 1 cut(s) 624
HinfI GANTC 3 cut(s) 806, 1080, 1117
HphI GGTGA 4 cut(s) 67, 135, 676, 1147
Hpy166II GTNNAC 1 cut(s) 1323
Hpy188I TCNGA 6 cut(s) 135, 220, 305, 706, 837, 1171
Hpy188III TCNNGA 9 cut(s) 343, 361, 686, 937, 970, 1114, 1143, 1240, 1251
Hpy8I GTNNAC 1 cut(s) 1323
HpyAV CCTTC 6 cut(s) 156, 187, 234, 358, 1403, 1456
HpyCH4III ACNGT 6 cut(s) 274, 358, 677, 772, 822, 943
HpyCH4IV ACGT 5 cut(s) 317, 332, 1003, 1037, 1325
HpyCH4V TGCA 9 cut(s) 32, 107, 269, 314, 518, 866, 1001, 1211, 1425
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 753, 1352
HpyF3I CTNAG 6 cut(s) 23, 259, 411, 976, 1195, 1202
HpySE526I ACGT 5 cut(s) 317, 332, 1003, 1037, 1325
Hsp92II CATG 9 cut(s) 189, 294, 346, 382, 428, 591, 920, 1030, 1090
Kzo9I GATC 4 cut(s) 89, 448, 1242, 1305
LguI GCTCTTC 1 cut(s) 737
LmnI GCTCC 3 cut(s) 38, 189, 342
Lsp1109I GCAGC 3 cut(s) 16, 768, 854
LweI GCATC 5 cut(s) 388, 824, 992, 1198, 1377
MaeI CTAG 1 cut(s) 812
MaeII ACGT 5 cut(s) 317, 332, 1003, 1037, 1325
MaeIII GTNAC 1 cut(s) 141
MalI GATC 4 cut(s) 91, 450, 1244, 1307
MbiI CCGCTC 1 cut(s) 430
MboI GATC 4 cut(s) 89, 448, 1242, 1305
MboII GAAGA 9 cut(s) 28, 144, 172, 542, 704, 721, 754, 1089, 1365
MfeI CAATTG 2 cut(s) 578, 1437
MflI RGATCY 2 cut(s) 89, 448
MhlI GDGCHC 5 cut(s) 279, 374, 998, 1188, 1283
MlyI GAGTC 1 cut(s) 1111
MseI TTAA 6 cut(s) 195, 353, 521, 647, 870, 1058
MslI CAYNNNNRTG 1 cut(s) 820
MspA1I CMGCKG 1 cut(s) 121
MspR9I CCNGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
MunI CAATTG 2 cut(s) 578, 1437
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
MwoI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 753, 1352
NcoI CCATGG 3 cut(s) 185, 290, 378
NdeI CATATG 1 cut(s) 491
NdeII GATC 4 cut(s) 89, 448, 1242, 1305
NlaIII CATG 9 cut(s) 189, 294, 346, 382, 428, 591, 920, 1030, 1090
NlaIV GGNNCC 4 cut(s) 40, 252, 338, 827
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 141
NspI RCATGY 1 cut(s) 920
PaeR7I CTCGAG 1 cut(s) 1143
PagI TCATGA 1 cut(s) 342
PasI CCCWGGG 1 cut(s) 79
PceI AGGCCT 1 cut(s) 1023
PciI ACATGT 1 cut(s) 916
PciSI GCTCTTC 1 cut(s) 737
PctI GAATGC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 806, 1080
PflMI CCANNNNNTGG 2 cut(s) 206, 367
PfoI TCCNGGA 2 cut(s) 1122, 1267
PkrI GCNGC 4 cut(s) 31, 488, 758, 844
PleI GAGTC 1 cut(s) 1111
PmaCI CACGTG 2 cut(s) 333, 1004
PmlI CACGTG 2 cut(s) 333, 1004
PpsI GAGTC 1 cut(s) 1111
Ppu21I YACGTR 2 cut(s) 333, 1004
PscI ACATGT 1 cut(s) 916
Psp1406I AACGTT 2 cut(s) 1037, 1325
Psp6I CCWGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
PspCI CACGTG 2 cut(s) 333, 1004
PspFI CCCAGC 1 cut(s) 1346
PspGI CCWGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
PspN4I GGNNCC 4 cut(s) 40, 252, 338, 827
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 89, 448
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 2 cut(s) 564, 1055
RsaNI GTAC 2 cut(s) 563, 1054
RseI CAYNNNNRTG 1 cut(s) 820
SapI GCTCTTC 1 cut(s) 737
SaqAI TTAA 6 cut(s) 195, 353, 521, 647, 870, 1058
SatI GCNGC 4 cut(s) 30, 487, 757, 843
Sau3AI GATC 4 cut(s) 89, 448, 1242, 1305
ScaI AGTACT 1 cut(s) 1055
SchI GAGTC 1 cut(s) 1111
ScrFI CCNGG 6 cut(s) 80, 368, 788, 911, 1124, 1269
SduI GDGCHC 5 cut(s) 279, 374, 998, 1188, 1283
SfaNI GCATC 5 cut(s) 388, 824, 992, 1198, 1377
SfcI CTRYAG 3 cut(s) 105, 612, 1470
Sfr274I CTCGAG 1 cut(s) 1143
SlaI CTCGAG 1 cut(s) 1143
SmiMI CAYNNNNRTG 1 cut(s) 820
SmlI CTYRAG 2 cut(s) 1095, 1143
SmoI CTYRAG 2 cut(s) 1095, 1143
SseBI AGGCCT 1 cut(s) 1023
SsiI CCGC 3 cut(s) 430, 486, 1482
SspMI CTAG 1 cut(s) 812
StuI AGGCCT 1 cut(s) 1023
StyD4I CCNGG 6 cut(s) 78, 366, 786, 909, 1122, 1267
StyI CCWWGG 3 cut(s) 185, 290, 378
TaaI ACNGT 6 cut(s) 274, 358, 677, 772, 822, 943
TaiI ACGT 5 cut(s) 320, 335, 1006, 1040, 1328
TaqI TCGA 4 cut(s) 170, 938, 1144, 1304
TatI WGTACW 2 cut(s) 562, 1053
TauI GCSGC 1 cut(s) 489
TfiI GAWTC 2 cut(s) 806, 1080
Tru1I TTAA 6 cut(s) 195, 353, 521, 647, 870, 1058
Tru9I TTAA 6 cut(s) 195, 353, 521, 647, 870, 1058
TscAI CASTG 2 cut(s) 827, 1003
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 3 cut(s) 29, 756, 842
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 5 cut(s) 426, 521, 705, 1082, 1420
TspRI CASTG 2 cut(s) 827, 1003
Van91I CCANNNNNTGG 2 cut(s) 206, 367
XapI RAATTY 3 cut(s) 210, 607, 635
XceI RCATGY 1 cut(s) 920
XhoI CTCGAG 1 cut(s) 1143
XspI CTAG 1 cut(s) 812
ZrmI AGTACT 1 cut(s) 1055
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.