FvH4_3g22370

BT1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0013256
Physical Location & Seq
Forward (+)
15397256 .. 15401682
4427 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g22370.t2

Sequence Viewer

Length: 1485 bp
ATGATAAAATGGCTGAGAAGATTAAGAGCTGCATTTGGAGCCTCGTTTCTCTGGCTCGTTTGCCTCATTTACTTCACCCAGGGTTTTAGATCCTTTGTTTGGACTGCAGTTTCATACCAGCTGAAAGATACGCTCAAGTTGTCACCTTCGTCTTCTCAGTTTGTGTTTTCCATAGCTTTCTTTCCGTGGAGCATCAAACCGTTATATGGAATTTTGTCTGATTGTATTCCAATAAAAGGAAGGAAAAGGGTTCCATACTTAGTGATTGCAACTGTGCTCTCTCTTGTCCCATGGCCTATTCTCGGACTGAATGCAATGTCAAGGAGTTCCATGTGGCTCCTCATGGCCTTCTTAACAGTCCAGAACCTGGGCTCGGCCATGGCTGATGTAGTAGTTGATGCAATGATTGCTGAGACAGTAAGACATGAGCGGGCTTCATTTGCTGGAGATCTCCAGTCAGTATCGTGGTTGGCAATGGCTTTCGGGGGCATATGTGGGAGTTTGACAGGAGGATTTGCATTATCCAACTTTGATATACACACAATTTTTCTTCTTTTTTCTATACTACCGTCCATACAATTGTTATCATGTGGCTTGGTGGAGGAGAATTCTGTAGATAGTGAAGCTTTTGCTGAATCCAAATTAAGAAGCTCACAGGGGGTGAATGGGAACCATAGTTCTCTAGATGAAGAAAGTTTTTCAGAGAAGAAATCTAATACCAGCATTACAAGGAGAAAGAAAAGCAAGAAGGGCAGCAAAAATAAAACAGTTTCAAGTAAAGTACAGGTTTCTGGGAAGCTTGACTCATTAGCATCACAGTGGTTCCAATCACTGAAAGCAGCCACTTTTAGTCTGTGCCGTGCATTTAAGCAACCAATTATTTTGAGATCAATGGCATGGTTTTTCCTGGCACACATTTCCATTCCAAACCTCTCAACTGTGATGTTTTATTATCAAACCGAATTCTTGAACTTAGAAGCATCCTTTTTGGGCACTGCACGTGTTGTGGGATGGTTAGGCCTCATGCTTGGAACCTTAGTATATAATCGGTTCTTAAAGCGAATGGAGTTGAGAAGAATCCTCATGTGGTCTCAACTTGGTTTGGCCTTCTTGACGCTGCTTGACATTGTTTTAGTGTCTCGAGTAAATCTCACCTTCGGTATATCAGATAGGATTATGGTGCTCTGTGGCTCAGCTCTTAGTGATGCAGTCAATCAGTTCAAGTTTATGCCATTCCTGATCTTATCTGGACAGCTTTGCCCTCCTGGAATCGAGGGAACTCTGTTTGCCCTTTTTATGTCAATAAATAATTTAGGGTCAACGTTGGGGTCCTTTGTCGGTGCTGGATTGGCTTCAGTTTTGAGCATTTCTTCAGGCTCTTTTGACAACCTTCTATTGGGAATTGTCATTCAAGTTCTCTGCATATTCATACCAATTGCTTTCCTATTTTTGATACCAAAGGAAGCTACAGGAATATCAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BT1

Protein Analysis

495

Amino Acids

54.43

Weight (kDa)

9.62

Isoelectric Point (pI)

40.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 25 - 398 3.6e-18 Major Facilitator Superfamily
BT1 PF03092 27 - 408 3.6e-73 BT1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013256)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G54860
fragaria_vesca FvH4_3g22370 FvH4_3g22370 FvH4_3g22370 FvH4_3g22370
malus_domestica MD03G1205600.v1.1
prunus_persica Prupe.4G210100_v2.0.a1
pyrus_communis pycom03g15530
rosa_chinensis RchiOBHm_Chr5g0039011
rosa_laevigata RLG00000033883
rosa_multiflora Rmu_sc0041879.1_g000001
rosa_roxburghii Rroxscaffold_1G00042110
rosa_rugosa Rorug05G0175600
rosa_samantha Rh5AG262300 Rh5BG266900 Rh5CG299500 Rh5DG273400
rosa_wichuraiana Rw5G024500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 367
AccBSI CCGCTC 1 cut(s) 430
AciI CCGC 1 cut(s) 430
AclI AACGTT 1 cut(s) 1322
AclWI GGATC 1 cut(s) 84
AcoI YGGCCR 1 cut(s) 375
AcsI RAATTY 3 cut(s) 210, 607, 962
AcuI CTGAAG 2 cut(s) 1338, 1356
AcvI CACGTG 1 cut(s) 1001
AfaI GTAC 1 cut(s) 783
AfiI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1396
AflIII ACRYGT 1 cut(s) 1000
AgsI TTSAA 4 cut(s) 774, 970, 1222, 1412
AjnI CCWGG 4 cut(s) 78, 366, 906, 1264
AloI GAACNNNNNNTCC 2 cut(s) 661, 693
Alw21I GWGCWC 2 cut(s) 279, 1185
Alw26I GTCTC 3 cut(s) 407, 1095, 1143
AlwI GGATC 1 cut(s) 84
AlwNI CAGNNNCTG 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 1140
AoxI GGCC 5 cut(s) 293, 345, 375, 1018, 1104
ApeKI GCWGC 4 cut(s) 29, 753, 839, 1117
ApoI RAATTY 3 cut(s) 210, 607, 962
AspS9I GGNCC 1 cut(s) 1329
AsuHPI GGTGA 4 cut(s) 67, 135, 673, 1144
AvaI CYCGRG 1 cut(s) 1140
AvaII GGWCC 1 cut(s) 1329
BaeGI GKGCMC 1 cut(s) 995
BanII GRGCYC 1 cut(s) 374
BbrPI CACGTG 1 cut(s) 1001
BbsI GAAGAC 1 cut(s) 144
Bbv12I GWGCWC 2 cut(s) 279, 1185
BbvI GCAGC 4 cut(s) 16, 765, 851, 1104
BccI CCATC 1 cut(s) 1005
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 4 cut(s) 80, 368, 908, 1266
BcoDI GTCTC 3 cut(s) 407, 1095, 1143
BfaI CTAG 1 cut(s) 683
BfmI CTRYAG 3 cut(s) 105, 612, 1467
BglII AGATCT 1 cut(s) 448
BisI GCNGC 4 cut(s) 30, 754, 840, 1118
BlpI GCTNAGC 1 cut(s) 1192
BlsI GCNGC 4 cut(s) 31, 755, 841, 1119
Bme1390I CCNGG 4 cut(s) 80, 368, 908, 1266
Bme18I GGWCC 1 cut(s) 1329
BmeT110I CYCGRG 1 cut(s) 1140
BmgT120I GGNCC 1 cut(s) 1329
BmiI GGNNCC 7 cut(s) 40, 252, 338, 671, 824, 1033, 1330
BmrFI CCNGG 4 cut(s) 80, 368, 908, 1266
BmsI GCATC 5 cut(s) 201, 388, 821, 989, 1195
BpiI GAAGAC 1 cut(s) 144
BplI GAGNNNNNCTC 2 cut(s) 1134, 1166
BpmI CTGGAG 2 cut(s) 437, 465
Bpu1102I GCTNAGC 1 cut(s) 1192
BpuEI CTTGAG 1 cut(s) 119
BsaAI YACGTR 1 cut(s) 1001
BsaI GGTCTC 1 cut(s) 1095
BsaJI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
Bsc4I CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1396
Bse1I ACTGG 1 cut(s) 454
Bse3DI GCAATG 3 cut(s) 321, 408, 480
BseBI CCWGG 4 cut(s) 80, 368, 908, 1266
BseDI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
BseGI GGATG 2 cut(s) 980, 1016
BseLI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1396
BseMI GCAATG 3 cut(s) 321, 408, 480
BseMII CTCAG 4 cut(s) 5, 170, 402, 1206
BseNI ACTGG 1 cut(s) 454
BseRI GAGGAG 2 cut(s) 329, 617
BseSI GKGCMC 1 cut(s) 995
BseXI GCAGC 4 cut(s) 16, 765, 851, 1104
BsgI GTGCAG 1 cut(s) 981
BshFI GGCC 5 cut(s) 295, 347, 377, 1020, 1106
BsiHKAI GWGCWC 2 cut(s) 279, 1185
BsiHKCI CYCGRG 1 cut(s) 1140
BslFI GGGAC 1 cut(s) 272
BslI CCNNNNNNNGG 7 cut(s) 99, 206, 236, 302, 367, 373, 1396
BsmAI GTCTC 3 cut(s) 407, 1095, 1143
BsmFI GGGAC 1 cut(s) 272
BsmI GAATGC 1 cut(s) 316
BsnI GGCC 5 cut(s) 295, 347, 377, 1020, 1106
Bso31I GGTCTC 1 cut(s) 1095
BsoBI CYCGRG 1 cut(s) 1140
Bsp1286I GDGCHC 4 cut(s) 279, 374, 995, 1185
Bsp143I GATC 4 cut(s) 89, 448, 887, 1239
Bsp1720I GCTNAGC 1 cut(s) 1192
Bsp19I CCATGG 2 cut(s) 290, 378
BspACI CCGC 1 cut(s) 430
BspANI GGCC 5 cut(s) 295, 347, 377, 1020, 1106
BspCNI CTCAG 4 cut(s) 6, 169, 403, 1205
BspLI GGNNCC 7 cut(s) 40, 252, 338, 671, 824, 1033, 1330
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 84
BspTNI GGTCTC 1 cut(s) 1095
BsrBI CCGCTC 1 cut(s) 430
BsrDI GCAATG 3 cut(s) 321, 408, 480
BsrI ACTGG 1 cut(s) 454
BssECI CCNNGG 6 cut(s) 78, 79, 185, 290, 367, 378
BssMI GATC 4 cut(s) 89, 448, 887, 1239
BssT1I CCWWGG 2 cut(s) 290, 378
Bst2UI CCWGG 4 cut(s) 80, 368, 908, 1266
Bst4CI ACNGT 8 cut(s) 201, 274, 358, 418, 570, 769, 819, 940
BstAPI GCANNNNNTGC 1 cut(s) 407
BstBAI YACGTR 1 cut(s) 1001
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 9 cut(s) 14, 156, 259, 411, 973, 1036, 1192, 1199, 1482
BstDSI CCRYGG 3 cut(s) 185, 290, 378
BstF5I GGATG 2 cut(s) 980, 1016
BstKTI GATC 4 cut(s) 92, 451, 890, 1242
BstMAI GTCTC 3 cut(s) 407, 1095, 1143
BstMBI GATC 4 cut(s) 89, 448, 887, 1239
BstMWI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 750, 1349
BstNI CCWGG 4 cut(s) 80, 368, 908, 1266
BstSCI CCNGG 4 cut(s) 78, 366, 906, 1264
BstSFI CTRYAG 3 cut(s) 105, 612, 1467
BstSLI GKGCMC 1 cut(s) 995
BstV1I GCAGC 4 cut(s) 16, 765, 851, 1104
BstV2I GAAGAC 1 cut(s) 144
BstX2I RGATCY 2 cut(s) 89, 448
BstYI RGATCY 2 cut(s) 89, 448
BsuRI GGCC 5 cut(s) 295, 347, 377, 1020, 1106
BtgI CCRYGG 3 cut(s) 185, 290, 378
BtsCI GGATG 2 cut(s) 980, 1016
BtsI GCAGTG 1 cut(s) 993
BtsIMutI CAGTG 3 cut(s) 824, 830, 993
Cac8I GCNNGC 1 cut(s) 432
CaiI CAGNNNCTG 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 1329
CseI GACGC 1 cut(s) 1123
Csp6I GTAC 1 cut(s) 782
CviAII CATG 9 cut(s) 291, 331, 343, 379, 425, 588, 897, 1024, 1084
CviQI GTAC 1 cut(s) 782
DdeI CTNAG 9 cut(s) 14, 156, 259, 411, 973, 1036, 1192, 1199, 1482
DpnI GATC 4 cut(s) 91, 450, 889, 1241
DpnII GATC 4 cut(s) 89, 448, 887, 1239
EaeI YGGCCR 1 cut(s) 375
Eco130I CCWWGG 2 cut(s) 290, 378
Eco147I AGGCCT 1 cut(s) 1020
Eco24I GRGCYC 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 1095
Eco47I GGWCC 1 cut(s) 1329
Eco57I CTGAAG 2 cut(s) 1338, 1356
Eco72I CACGTG 1 cut(s) 1001
Eco88I CYCGRG 1 cut(s) 1140
EcoO109I RGGNCCY 1 cut(s) 1329
EcoRI GAATTC 2 cut(s) 607, 962
EcoRII CCWGG 4 cut(s) 78, 366, 906, 1264
EcoT14I CCWWGG 2 cut(s) 290, 378
EcoT38I GRGCYC 1 cut(s) 374
ErhI CCWWGG 2 cut(s) 290, 378
FaeI CATG 9 cut(s) 294, 334, 346, 382, 428, 591, 900, 1027, 1087
FaqI GGGAC 1 cut(s) 272
FatI CATG 9 cut(s) 290, 330, 342, 378, 424, 587, 896, 1023, 1083
FauI CCCGC 1 cut(s) 423
FauNDI CATATG 1 cut(s) 491
Fnu4HI GCNGC 4 cut(s) 30, 754, 840, 1118
FokI GGATG 2 cut(s) 967, 1023
FriOI GRGCYC 1 cut(s) 374
Fsp4HI GCNGC 4 cut(s) 30, 754, 840, 1118
FspBI CTAG 1 cut(s) 683
GluI GCNGC 4 cut(s) 30, 754, 840, 1118
GsuI CTGGAG 2 cut(s) 437, 465
HaeIII GGCC 5 cut(s) 295, 347, 377, 1020, 1106
HgaI GACGC 1 cut(s) 1123
Hin1II CATG 9 cut(s) 294, 334, 346, 382, 428, 591, 900, 1027, 1087
HincII GTYRAC 1 cut(s) 1320
HindII GTYRAC 1 cut(s) 1320
HindIII AAGCTT 2 cut(s) 624, 797
HinfI GANTC 4 cut(s) 635, 803, 1077, 1269
HphI GGTGA 4 cut(s) 67, 135, 673, 1144
Hpy166II GTNNAC 1 cut(s) 1320
Hpy188I TCNGA 4 cut(s) 220, 305, 703, 1168
Hpy188III TCNNGA 7 cut(s) 361, 683, 967, 1111, 1140, 1237, 1248
Hpy8I GTNNAC 1 cut(s) 1320
HpyAV CCTTC 7 cut(s) 156, 234, 358, 742, 1117, 1165, 1400
HpyCH4III ACNGT 8 cut(s) 201, 274, 358, 418, 570, 769, 819, 940
HpyCH4IV ACGT 2 cut(s) 1000, 1322
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 750, 1349
HpyF3I CTNAG 9 cut(s) 14, 156, 259, 411, 973, 1036, 1192, 1199, 1482
HpySE526I ACGT 2 cut(s) 1000, 1322
Hsp92II CATG 9 cut(s) 294, 334, 346, 382, 428, 591, 900, 1027, 1087
Kzo9I GATC 4 cut(s) 89, 448, 887, 1239
LmnI GCTCC 3 cut(s) 38, 189, 342
Lsp1109I GCAGC 4 cut(s) 16, 765, 851, 1104
LweI GCATC 5 cut(s) 201, 388, 821, 989, 1195
MaeI CTAG 1 cut(s) 683
MaeII ACGT 2 cut(s) 1000, 1322
MaeIII GTNAC 1 cut(s) 141
MalI GATC 4 cut(s) 91, 450, 889, 1241
MbiI CCGCTC 1 cut(s) 430
MboI GATC 4 cut(s) 89, 448, 887, 1239
MboII GAAGA 7 cut(s) 30, 144, 542, 701, 718, 1086, 1362
MfeI CAATTG 2 cut(s) 578, 1434
MflI RGATCY 2 cut(s) 89, 448
MhlI GDGCHC 4 cut(s) 279, 374, 995, 1185
MlyI GAGTC 1 cut(s) 797
MmeI TCCRAC 1 cut(s) 549
MseI TTAA 5 cut(s) 23, 353, 644, 867, 1055
MslI CAYNNNNRTG 1 cut(s) 817
MspA1I CMGCKG 1 cut(s) 121
MspR9I CCNGG 4 cut(s) 80, 368, 908, 1266
MunI CAATTG 2 cut(s) 578, 1434
Mva1269I GAATGC 1 cut(s) 316
MvaI CCWGG 4 cut(s) 80, 368, 908, 1266
MwoI GCNNNNNNNGC 5 cut(s) 38, 407, 440, 750, 1349
NcoI CCATGG 2 cut(s) 290, 378
NdeI CATATG 1 cut(s) 491
NdeII GATC 4 cut(s) 89, 448, 887, 1239
NlaIII CATG 9 cut(s) 294, 334, 346, 382, 428, 591, 900, 1027, 1087
NlaIV GGNNCC 7 cut(s) 40, 252, 338, 671, 824, 1033, 1330
NmeAIII GCCGAG 1 cut(s) 353
NmuCI GTSAC 1 cut(s) 141
PaeR7I CTCGAG 1 cut(s) 1140
PasI CCCWGGG 1 cut(s) 79
PceI AGGCCT 1 cut(s) 1020
PctI GAATGC 1 cut(s) 316
PfeI GAWTC 3 cut(s) 635, 1077, 1269
PflMI CCANNNNNTGG 1 cut(s) 367
PfoI TCCNGGA 1 cut(s) 1264
PkrI GCNGC 4 cut(s) 31, 755, 841, 1119
PleI GAGTC 1 cut(s) 797
PmaCI CACGTG 1 cut(s) 1001
PmlI CACGTG 1 cut(s) 1001
PpsI GAGTC 1 cut(s) 797
Ppu21I YACGTR 1 cut(s) 1001
PpuMI RGGWCCY 1 cut(s) 1329
Psp1406I AACGTT 1 cut(s) 1322
Psp5II RGGWCCY 1 cut(s) 1329
Psp6I CCWGG 4 cut(s) 78, 366, 906, 1264
PspCI CACGTG 1 cut(s) 1001
PspGI CCWGG 4 cut(s) 78, 366, 906, 1264
PspN4I GGNNCC 7 cut(s) 40, 252, 338, 671, 824, 1033, 1330
PspPI GGNCC 1 cut(s) 1329
PspPPI RGGWCCY 1 cut(s) 1329
PstI CTGCAG 1 cut(s) 109
PstNI CAGNNNCTG 1 cut(s) 367
PsuI RGATCY 2 cut(s) 89, 448
PvuII CAGCTG 1 cut(s) 121
RsaI GTAC 1 cut(s) 783
RsaNI GTAC 1 cut(s) 782
RseI CAYNNNNRTG 1 cut(s) 817
SaqAI TTAA 5 cut(s) 23, 353, 644, 867, 1055
SatI GCNGC 4 cut(s) 30, 754, 840, 1118
Sau3AI GATC 4 cut(s) 89, 448, 887, 1239
Sau96I GGNCC 1 cut(s) 1329
SchI GAGTC 1 cut(s) 797
ScrFI CCNGG 4 cut(s) 80, 368, 908, 1266
SduI GDGCHC 4 cut(s) 279, 374, 995, 1185
SfaNI GCATC 5 cut(s) 201, 388, 821, 989, 1195
SfcI CTRYAG 3 cut(s) 105, 612, 1467
Sfr274I CTCGAG 1 cut(s) 1140
SinI GGWCC 1 cut(s) 1329
SlaI CTCGAG 1 cut(s) 1140
SmiMI CAYNNNNRTG 1 cut(s) 817
SmlI CTYRAG 2 cut(s) 134, 1140
SmoI CTYRAG 2 cut(s) 134, 1140
SseBI AGGCCT 1 cut(s) 1020
SsiI CCGC 1 cut(s) 430
SspMI CTAG 1 cut(s) 683
StuI AGGCCT 1 cut(s) 1020
StyD4I CCNGG 4 cut(s) 78, 366, 906, 1264
StyI CCWWGG 2 cut(s) 290, 378
TaaI ACNGT 8 cut(s) 201, 274, 358, 418, 570, 769, 819, 940
TaiI ACGT 2 cut(s) 1003, 1325
TaqI TCGA 2 cut(s) 1141, 1272
TatI WGTACW 1 cut(s) 781
TfiI GAWTC 3 cut(s) 635, 1077, 1269
Tru1I TTAA 5 cut(s) 23, 353, 644, 867, 1055
Tru9I TTAA 5 cut(s) 23, 353, 644, 867, 1055
TscAI CASTG 3 cut(s) 824, 837, 1000
TseFI GTSAC 1 cut(s) 141
TseI GCWGC 4 cut(s) 29, 753, 839, 1117
Tsp45I GTSAC 1 cut(s) 141
TspDTI ATGAA 4 cut(s) 102, 426, 702, 1417
TspGWI ACGGA 1 cut(s) 174
TspRI CASTG 3 cut(s) 824, 837, 1000
Van91I CCANNNNNTGG 1 cut(s) 367
VpaK11BI GGWCC 1 cut(s) 1329
XapI RAATTY 3 cut(s) 210, 607, 962
XbaI TCTAGA 1 cut(s) 682
XhoI CTCGAG 1 cut(s) 1140
XspI CTAG 1 cut(s) 683
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.