AT5G56250

hap8 hap8 (hapless 8)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002524
Physical Location & Seq
Reverse (-)
22768453 .. 22773021
4569 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56250.2

Sequence Viewer

Length: 2436 bp
ATGTTCTTATCATCCACTGATCAAAGAAGCCCTTCTAATAACAATCCATCATCTTCTTCTAGTTTCTTACATCTGACCAACTCTTGTGATGAGTTAGGTCAATCACATCTTTCAACTTTCTCCATCAGAGATTATGCATTTAGTTACCGGACCAAGAACATCAAGAAAAGTTGGCCTTTTTCCTCAACAAGTTTGCAACTTTGTTTAAATCATGGCTTAACTGATCCATTGCCACCGATTCAGCCTCCTGAAGTGAAGAAGCCAAACATAACTCATGTGGAAGCGGTAAGTCACAAGAGAAAGTCAGAGAAACTTGGCTCTTATCAGATATTAGTAGAGACTACTAAACAAGGATTTGAGAATGGTTTGTTGGCGAGTGGTTCGAAATCCAAAATACAAGTAGCTATGGTTAACAAGAATCCTAGAAAAAAGTGTGGGTTGATAGTGAAACCTGGAGCTTGTGTGGATAGTGGTGGAAAAGAAGATCATAGTAGCCTCTTTTCAGCTTCTGATTCTATGGCTTTGAGAACTTGTCCCATCTGCAAAACCTTCTCGTCTGCTTCTAACACTACTTTAAATGCGCACATTGATCAGTGTCTGTCTGTTGACTCGGGGCAGCAGCCGATTCGTAAGCCAAACAGGCCTAAGACTAAGCCACGGTTGAAAGTTAAAACCATGACTGATATCTATGCTTCTGCGAAAGAAGGCACATTAGAAGATCTTGATAAAAGAAATGGCACAAAGTGGGCTATGATTTCGAGCTACTCTAACCGTGTTGTCTCTGATGATAAGCCTGAAGTGTCCAACAAAGTAAAGAAGCGAAGCGTGTCGCGTGCTCGCATTGATGAAGATGCTGCTGGCATTGGACCTGTTTACATTGATGCTAAGGGCCAAAAACTGCGGATTTTATCTAAGTTTAACGAGAAAGCTTCTGATCCATCAAGAAGAGAGCATGAAGAAGTTTGTGAGAAGAAATCTTCTAGTGAAGGTAAAGGGGGCAAAAGCTTTAGGAAAAAACTTTGGGGAGAGAAACATTACAAGCATCGTAAACTAGTTCCTCAAAACAGAAAACTCACTGTTCGTAAAAGCAATGCTTCAGAGATACCGGAGTATCGAAGAGGATATTCTAAAGAAGGTAAAGACTTTGAGAGGTCAGAAACTTCAGGTCCTGGTCAAGGTAGAATATTCAACCAGCGGATGCTAACAAAGCGTTCTCTTTCAAGGCATGGAAAGAAGAATGGGACAGACATTTGTGAATCTGAGAATTGGAATTCATTGTCAGAAGATCCTCTTGTGTTAAGAAGTCCAAGTCATGTGAGTACTGATTTGTCCGAGACAGTTTCTTCTCCTTTAAACAGTATAGGGTCATGGAGAGTTTGTGGGGAGAGCCAAGTTTCTGGAAAGAGCTGGGCTTTGTCTAGGAACAGAAGCATTGAAAGTGATTTATTTGTAGCAAATCCCTTGAGATGTTTAACACCTGTGGCGAGAGGCGTCATGAAGTTCAAGAAAGCTCGAATGGATTTCTCTGAGAATGAAGATGAAGATATAGGGAAATGGGAGTCTGAAATGACTCAAGAACGTGAACTGTCAGATTATGATGGTTGGGATGATGATGATGGAGAAACAGATAAGGTTGCGCTAAGCTCAAATCCTTCGTTTAGCGGGGAAGACAATGATTATGAAAGCTATGAAGAGACTGGTGATAACAAAGGAGGTGATGATATGTTGGACAAAACTAAAGATGCAGATGTTGAGTTTGAAAGCATGGTTTATGAGAAAACTGGTTGCGAAACCGCGGAACAAGAAAGCTCGTTCATGGAGGTTGATCCCATTCCTATTCCAGGACCTCCGGGATCATTTTTACCAAGTCCTTGGGATATGATGGGAACTGATGCAGTTGAACATCATGGAAACTCTTCAGTTATCACAAGCCAAGTCCACTCTTCACAAGATCAGTTTGATCTCACTGACAGAAATTCATCGGAATCACCTGTTTCAGCAATTTCAAACTTTGCAGCTCCTGAAACACAGACATTAAGTCTTCATAACATCATCACCACTGACAAGAGACCTTCCAGATTCAGAGACAATGATCAATCATGTTGTTGCCAAAGAAAGGAGAAAGCTCTTGAAGATACAACATTTTGTCAACCACCACCACCACAAATGATCCAACAAGATTTGGATTTCCTGAGTAAGTCTGTGCCTGCGGTTCCTTCAACTCCCAACCCGGTGTTGCGGCTGATGGGAAAGGATTTGATGGTTATTAACCAAAGAGAAGAGCCTTCACACAACGAATCAAGTCCAAAACCAACCTCTCAGTTTCTAGATCTTTCTAAGACACAGCAAGCGTCTCCTTCTGTTAATCTTCTTCATCCTCCTTACAGCGGAAATGGTTATTTCGACACCAATACAAGCTTCTACAACATCCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

811

Amino Acids

90.29

Weight (kDa)

6.68

Isoelectric Point (pI)

49.7

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 582
AccII CGCG 2 cut(s) 832, 1796
AciI CCGC 9 cut(s) 284, 901, 1195, 1662, 1794, 1796, 2210, 2239, 2388
AclWI GGATC 6 cut(s) 218, 929, 1280, 1820, 1861, 2164
AcsI RAATTY 2 cut(s) 1270, 1975
AcuI CTGAAG 5 cut(s) 270, 816, 1080, 1146, 1902
AcyI GRCGYC 1 cut(s) 1491
AdeI CACNNNGTG 1 cut(s) 744
AfaI GTAC 1 cut(s) 1321
AfiI CCNNNNNNNGG 4 cut(s) 1175, 1841, 2116, 2387
AhdI GACNNNNNGTC 1 cut(s) 2037
AhlI ACTAGT 1 cut(s) 1051
AjnI CCWGG 3 cut(s) 451, 1168, 1840
AjuI GAANNNNNNNTTGG 4 cut(s) 353, 385, 2402, 2434
Alw21I GWGCWC 1 cut(s) 838
Alw26I GTCTC 7 cut(s) 332, 784, 1328, 1688, 2062, 2079, 2358
AlwI GGATC 6 cut(s) 218, 929, 1280, 1820, 1861, 2164
AlwNI CAGNNNCTG 4 cut(s) 509, 598, 1169, 2021
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 3 cut(s) 173, 641, 889
ApeKI GCWGC 4 cut(s) 616, 619, 854, 2015
ApoI RAATTY 2 cut(s) 1270, 1975
Asp700I GAANNNNTTC 2 cut(s) 31, 1915
AspLEI GCGC 2 cut(s) 583, 1639
AspS9I GGNCC 5 cut(s) 150, 866, 889, 1166, 1844
AsuC2I CCSGG 2 cut(s) 1851, 2231
AsuHPI GGTGA 4 cut(s) 1712, 1727, 1980, 2047
AsuII TTCGAA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 4 cut(s) 150, 866, 1166, 1844
BaeI ACNNNNGTAYC 4 cut(s) 1094, 1094, 1127, 1127
BbsI GAAGAC 2 cut(s) 1674, 2033
Bbv12I GWGCWC 1 cut(s) 838
BbvI GCAGC 4 cut(s) 628, 631, 841, 2027
BccI CCATC 9 cut(s) 55, 131, 545, 946, 1592, 1610, 1876, 2239, 2254
BcgI CGANNNNNNTGC 2 cut(s) 608, 642
BciT130I CCWGG 3 cut(s) 453, 1170, 1842
BclI TGATCA 3 cut(s) 19, 589, 2092
BcnI CCSGG 2 cut(s) 1851, 2231
BcoDI GTCTC 7 cut(s) 332, 784, 1328, 1688, 2062, 2079, 2358
BcuI ACTAGT 1 cut(s) 1051
BfaI CTAG 6 cut(s) 60, 423, 981, 1052, 1419, 2327
BglI GCCNNNNNGGC 1 cut(s) 640
BglII AGATCT 2 cut(s) 718, 2329
BisI GCNGC 5 cut(s) 617, 620, 855, 2016, 2240
BlpI GCTNAGC 1 cut(s) 1640
BlsI GCNGC 5 cut(s) 618, 621, 856, 2017, 2241
BmcAI AGTACT 1 cut(s) 1321
Bme1390I CCNGG 5 cut(s) 453, 1170, 1842, 1851, 2231
Bme18I GGWCC 4 cut(s) 150, 866, 1166, 1844
BmeRI GACNNNNNGTC 1 cut(s) 2037
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 5 cut(s) 150, 866, 889, 1166, 1844
BmiI GGNNCC 1 cut(s) 2214
BmrFI CCNGG 5 cut(s) 453, 1170, 1842, 1851, 2231
BmsI GCATC 6 cut(s) 841, 871, 1051, 1188, 1732, 1882
BpiI GAAGAC 2 cut(s) 1674, 2033
BpmI CTGGAG 1 cut(s) 474
Bpu10I CCTNAGC 1 cut(s) 885
Bpu1102I GCTNAGC 1 cut(s) 1640
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 2 cut(s) 1483, 1557
BpuMI CCSGG 2 cut(s) 1851, 2231
BsaHI GRCGYC 1 cut(s) 1491
BsaI GGTCTC 1 cut(s) 2062
BsaJI CCNNGG 3 cut(s) 656, 1794, 1871
BsaWI WCCGGW 2 cut(s) 147, 1105
BsaXI ACNNNNNCTCC 4 cut(s) 447, 477, 1363, 1393
Bsc4I CCNNNNNNNGG 4 cut(s) 1175, 1841, 2116, 2387
Bse1I ACTGG 2 cut(s) 1702, 1786
Bse3DI GCAATG 2 cut(s) 227, 1096
BseBI CCWGG 3 cut(s) 453, 1170, 1842
BseDI CCNNGG 3 cut(s) 656, 1794, 1871
BseGI GGATG 5 cut(s) 11, 1203, 1612, 2374, 2427
BseLI CCNNNNNNNGG 4 cut(s) 1175, 1841, 2116, 2387
BseMI GCAATG 2 cut(s) 227, 1096
BseMII CTCAG 4 cut(s) 1251, 1518, 2183, 2333
BseNI ACTGG 2 cut(s) 1702, 1786
BseXI GCAGC 4 cut(s) 628, 631, 841, 2027
BseYI CCCAGC 1 cut(s) 1407
Bsh1236I CGCG 2 cut(s) 832, 1796
BshFI GGCC 3 cut(s) 175, 643, 891
BsiHKAI GWGCWC 1 cut(s) 838
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 4 cut(s) 148, 1106, 1850, 2231
BslFI GGGAC 2 cut(s) 519, 1255
BslI CCNNNNNNNGG 4 cut(s) 1175, 1841, 2116, 2387
BsmAI GTCTC 7 cut(s) 332, 784, 1328, 1688, 2062, 2079, 2358
BsmBI CGTCTC 1 cut(s) 2358
BsmFI GGGAC 2 cut(s) 519, 1255
BsnI GGCC 3 cut(s) 175, 643, 891
Bso31I GGTCTC 1 cut(s) 2062
BsoBI CYCGRG 1 cut(s) 610
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 1 cut(s) 838
Bsp1720I GCTNAGC 1 cut(s) 1640
BspACI CCGC 9 cut(s) 284, 901, 1195, 1662, 1794, 1796, 2210, 2239, 2388
BspANI GGCC 3 cut(s) 175, 643, 891
BspCNI CTCAG 4 cut(s) 1252, 1519, 2184, 2332
BspFNI CGCG 2 cut(s) 832, 1796
BspHI TCATGA 1 cut(s) 1494
BspLI GGNNCC 1 cut(s) 2214
BspPI GGATC 6 cut(s) 218, 929, 1280, 1820, 1861, 2164
BspQI GCTCTTC 1 cut(s) 2274
BspT104I TTCGAA 1 cut(s) 383
BspTNI GGTCTC 1 cut(s) 2062
BsrDI GCAATG 2 cut(s) 227, 1096
BsrI ACTGG 2 cut(s) 1702, 1786
BssECI CCNNGG 3 cut(s) 656, 1794, 1871
BssNI GRCGYC 1 cut(s) 1491
BssT1I CCWWGG 1 cut(s) 1871
Bst2UI CCWGG 3 cut(s) 453, 1170, 1842
Bst4CI ACNGT 6 cut(s) 660, 773, 1078, 1339, 1358, 1587
Bst6I CTCTTC 6 cut(s) 940, 1111, 1686, 1921, 1948, 2274
BstACI GRCGYC 1 cut(s) 1491
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 5 cut(s) 834, 838, 859, 2208, 2349
BstDSI CCRYGG 2 cut(s) 656, 1794
BstENI CCTNNNNNAGG 2 cut(s) 1173, 1839
BstF5I GGATG 5 cut(s) 11, 1203, 1612, 2374, 2427
BstFNI CGCG 2 cut(s) 832, 1796
BstHHI GCGC 2 cut(s) 583, 1639
BstMAI GTCTC 7 cut(s) 332, 784, 1328, 1688, 2062, 2079, 2358
BstMWI GCNNNNNNNGC 2 cut(s) 640, 1207
BstNI CCWGG 3 cut(s) 453, 1170, 1842
BstSCI CCNGG 5 cut(s) 451, 1168, 1840, 1849, 2229
BstUI CGCG 2 cut(s) 832, 1796
BstV1I GCAGC 4 cut(s) 628, 631, 841, 2027
BstV2I GAAGAC 2 cut(s) 1674, 2033
BstX2I RGATCY 3 cut(s) 718, 1285, 2329
BstXI CCANNNNNNTGG 2 cut(s) 1397, 1872
BstYI RGATCY 3 cut(s) 718, 1285, 2329
BsuRI GGCC 3 cut(s) 175, 643, 891
BtgI CCRYGG 2 cut(s) 656, 1794
BtsCI GGATG 5 cut(s) 11, 1203, 1612, 2374, 2427
BtsIMutI CAGTG 5 cut(s) 15, 599, 1074, 1965, 2058
Cac8I GCNNGC 5 cut(s) 834, 838, 859, 2208, 2349
CaiI CAGNNNCTG 4 cut(s) 509, 598, 1169, 2021
CciI TCATGA 1 cut(s) 1494
CfoI GCGC 2 cut(s) 583, 1639
Cfr13I GGNCC 5 cut(s) 150, 866, 889, 1166, 1844
Cfr42I CCGCGG 1 cut(s) 1797
CseI GACGC 2 cut(s) 1480, 2340
Csp6I GTAC 1 cut(s) 1320
CviQI GTAC 1 cut(s) 1320
DraI TTTAAA 3 cut(s) 207, 576, 1353
DraIII CACNNNGTG 1 cut(s) 744
DriI GACNNNNNGTC 1 cut(s) 2037
Eam1104I CTCTTC 6 cut(s) 940, 1111, 1686, 1921, 1948, 2274
Eam1105I GACNNNNNGTC 1 cut(s) 2037
EarI CTCTTC 6 cut(s) 940, 1111, 1686, 1921, 1948, 2274
Eco130I CCWWGG 1 cut(s) 1871
Eco147I AGGCCT 1 cut(s) 643
Eco31I GGTCTC 1 cut(s) 2062
Eco32I GATATC 1 cut(s) 685
Eco47I GGWCC 4 cut(s) 150, 866, 1166, 1844
Eco57I CTGAAG 5 cut(s) 270, 816, 1080, 1146, 1902
Eco88I CYCGRG 1 cut(s) 610
EcoNI CCTNNNNNAGG 2 cut(s) 1173, 1839
EcoO109I RGGNCCY 2 cut(s) 1166, 1844
EcoRI GAATTC 1 cut(s) 1270
EcoRII CCWGG 3 cut(s) 451, 1168, 1840
EcoRV GATATC 1 cut(s) 685
EcoT14I CCWWGG 1 cut(s) 1871
EcoT22I ATGCAT 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 1871
Esp3I CGTCTC 1 cut(s) 2358
FalI AAGNNNNNCTT 8 cut(s) 16, 48, 160, 192, 1078, 1110, 1275, 1307
FaqI GGGAC 2 cut(s) 519, 1255
FauI CCCGC 1 cut(s) 1655
FbaI TGATCA 3 cut(s) 19, 589, 2092
Fnu4HI GCNGC 5 cut(s) 617, 620, 855, 2016, 2240
FokI GGATG 4 cut(s) 1210, 1619, 2361, 2414
Fsp4HI GCNGC 5 cut(s) 617, 620, 855, 2016, 2240
FspAI RTGCGCAY 1 cut(s) 582
FspBI CTAG 6 cut(s) 60, 423, 981, 1052, 1419, 2327
FspI TGCGCA 1 cut(s) 582
GlaI GCGC 2 cut(s) 582, 1638
GluI GCNGC 5 cut(s) 617, 620, 855, 2016, 2240
GsaI CCCAGC 1 cut(s) 1411
GsuI CTGGAG 1 cut(s) 474
HaeIII GGCC 3 cut(s) 175, 643, 891
HapII CCGG 4 cut(s) 148, 1106, 1850, 2231
HgaI GACGC 2 cut(s) 1480, 2340
HhaI GCGC 2 cut(s) 583, 1639
Hin1I GRCGYC 1 cut(s) 1491
Hin6I GCGC 2 cut(s) 581, 1637
HinP1I GCGC 2 cut(s) 581, 1637
HincII GTYRAC 3 cut(s) 412, 607, 2150
HindII GTYRAC 3 cut(s) 412, 607, 2150
HindIII AAGCTT 3 cut(s) 927, 1003, 2416
HpaI GTTAAC 1 cut(s) 412
HpaII CCGG 4 cut(s) 148, 1106, 1850, 2231
HphI GGTGA 4 cut(s) 1712, 1727, 1980, 2047
Hpy166II GTNNAC 7 cut(s) 412, 607, 874, 1049, 1583, 1939, 2150
Hpy8I GTNNAC 7 cut(s) 412, 607, 874, 1049, 1583, 1939, 2150
HpyCH4III ACNGT 6 cut(s) 660, 773, 1078, 1339, 1358, 1587
HpyCH4IV ACGT 1 cut(s) 1579
HpyCH4V TGCA 6 cut(s) 137, 196, 543, 1745, 1895, 2015
HpyF10VI GCNNNNNNNGC 2 cut(s) 640, 1207
HpySE526I ACGT 1 cut(s) 1579
Hsp92I GRCGYC 1 cut(s) 1491
HspAI GCGC 2 cut(s) 581, 1637
Ksp22I TGATCA 3 cut(s) 19, 589, 2092
KspAI GTTAAC 1 cut(s) 412
KspI CCGCGG 1 cut(s) 1797
LguI GCTCTTC 1 cut(s) 2274
LmnI GCTCC 2 cut(s) 455, 2023
Lsp1109I GCAGC 4 cut(s) 628, 631, 841, 2027
LweI GCATC 6 cut(s) 841, 871, 1051, 1188, 1732, 1882
MaeI CTAG 6 cut(s) 60, 423, 981, 1052, 1419, 2327
MaeII ACGT 1 cut(s) 1579
MaeIII GTNAC 2 cut(s) 143, 290
MflI RGATCY 3 cut(s) 718, 1285, 2329
MhlI GDGCHC 1 cut(s) 838
MluCI AATT 4 cut(s) 1264, 1270, 1975, 2001
MlyI GAGTC 3 cut(s) 602, 1564, 1568
MmeI TCCRAC 3 cut(s) 828, 1707, 2197
Mph1103I ATGCAT 1 cut(s) 139
MroXI GAANNNNTTC 2 cut(s) 31, 1915
MspA1I CMGCKG 3 cut(s) 1195, 1796, 2388
MspI CCGG 4 cut(s) 148, 1106, 1850, 2231
MspR9I CCNGG 5 cut(s) 453, 1170, 1842, 1851, 2231
MvaI CCWGG 3 cut(s) 453, 1170, 1842
MvnI CGCG 2 cut(s) 832, 1796
MwoI GCNNNNNNNGC 2 cut(s) 640, 1207
NciI CCSGG 2 cut(s) 1851, 2231
NlaIV GGNNCC 1 cut(s) 2214
NmuCI GTSAC 1 cut(s) 290
NsbI TGCGCA 1 cut(s) 582
NsiI ATGCAT 1 cut(s) 139
NspV TTCGAA 1 cut(s) 383
PagI TCATGA 1 cut(s) 1494
PceI AGGCCT 1 cut(s) 643
PciSI GCTCTTC 1 cut(s) 2274
PdmI GAANNNNTTC 2 cut(s) 31, 1915
PfeI GAWTC 8 cut(s) 238, 418, 512, 625, 1256, 1985, 2079, 2297
PfoI TCCNGGA 2 cut(s) 1840, 1849
PkrI GCNGC 5 cut(s) 618, 621, 856, 2017, 2241
PleI GAGTC 3 cut(s) 602, 1564, 1567
PpsI GAGTC 3 cut(s) 602, 1564, 1567
PpuMI RGGWCCY 2 cut(s) 1166, 1844
Psp5II RGGWCCY 2 cut(s) 1166, 1844
Psp6I CCWGG 3 cut(s) 451, 1168, 1840
PspFI CCCAGC 1 cut(s) 1407
PspGI CCWGG 3 cut(s) 451, 1168, 1840
PspN4I GGNNCC 1 cut(s) 2214
PspPI GGNCC 5 cut(s) 150, 866, 889, 1166, 1844
PspPPI RGGWCCY 2 cut(s) 1166, 1844
PstNI CAGNNNCTG 4 cut(s) 509, 598, 1169, 2021
PsuI RGATCY 3 cut(s) 718, 1285, 2329
RsaI GTAC 1 cut(s) 1321
RsaNI GTAC 1 cut(s) 1320
SacII CCGCGG 1 cut(s) 1797
SapI GCTCTTC 1 cut(s) 2274
SatI GCNGC 5 cut(s) 617, 620, 855, 2016, 2240
Sau96I GGNCC 5 cut(s) 150, 866, 889, 1166, 1844
ScaI AGTACT 1 cut(s) 1321
SchI GAGTC 3 cut(s) 602, 1564, 1568
ScrFI CCNGG 5 cut(s) 453, 1170, 1842, 1851, 2231
SduI GDGCHC 1 cut(s) 838
SfaNI GCATC 6 cut(s) 841, 871, 1051, 1188, 1732, 1882
Sfr303I CCGCGG 1 cut(s) 1797
SfuI TTCGAA 1 cut(s) 383
SgrBI CCGCGG 1 cut(s) 1797
SinI GGWCC 4 cut(s) 150, 866, 1166, 1844
SmlI CTYRAG 2 cut(s) 1462, 1572
SmoI CTYRAG 2 cut(s) 1462, 1572
SpeI ACTAGT 1 cut(s) 1051
Sse9I AATT 4 cut(s) 1264, 1270, 1975, 2001
SseBI AGGCCT 1 cut(s) 643
SsiI CCGC 9 cut(s) 284, 901, 1195, 1662, 1794, 1796, 2210, 2239, 2388
SspI AATATT 1 cut(s) 1185
SspMI CTAG 6 cut(s) 60, 423, 981, 1052, 1419, 2327
StuI AGGCCT 1 cut(s) 643
StyD4I CCNGG 5 cut(s) 451, 1168, 1840, 1849, 2229
StyI CCWWGG 1 cut(s) 1871
TaaI ACNGT 6 cut(s) 660, 773, 1078, 1339, 1358, 1587
TaiI ACGT 1 cut(s) 1582
TaqI TCGA 5 cut(s) 383, 758, 1114, 1513, 2403
TasI AATT 4 cut(s) 1264, 1270, 1975, 2001
TatI WGTACW 1 cut(s) 1319
TauI GCSGC 1 cut(s) 2242
TfiI GAWTC 8 cut(s) 238, 418, 512, 625, 1256, 1985, 2079, 2297
TscAI CASTG 5 cut(s) 22, 599, 1081, 1972, 2065
TseFI GTSAC 1 cut(s) 290
TseI GCWGC 4 cut(s) 616, 619, 854, 2015
Tsp45I GTSAC 1 cut(s) 290
TspRI CASTG 5 cut(s) 22, 599, 1081, 1972, 2065
VpaK11BI GGWCC 4 cut(s) 150, 866, 1166, 1844
XagI CCTNNNNNAGG 2 cut(s) 1173, 1839
XapI RAATTY 2 cut(s) 1270, 1975
XbaI TCTAGA 1 cut(s) 2326
XmnI GAANNNNTTC 2 cut(s) 31, 1915
XspI CTAG 6 cut(s) 60, 423, 981, 1052, 1419, 2327
ZrmI AGTACT 1 cut(s) 1321
Zsp2I ATGCAT 1 cut(s) 139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.