AT5G59490

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0019565
Physical Location & Seq
Reverse (-)
23982621 .. 23984656
2036 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59490.1

Sequence Viewer

Length: 801 bp
ATGGAATTTGTGAACAGCTCTCCACCAAGATATGAATGTCTCCTCTTTGATTTGGATGATACTCTTTATCCTTTAAGCTCTGGCTTATCAGATGCATGCTCCAACAACATTATAGAATATATGGTTGAAAAGCTTGGAATAGATGAAGATGGAGTCGTTGAACTAAACCAAATTCTTTATAAGAAATATGGAACTTCAATGGCCGGTCTTAAGGCGGTAGGTTATGAATTCGATAATGACGAGTACCACCGGTATGTTCATGGTAGATTACCTTATGAAAACCTTAAACCGGACCCGGTTCTAAGGAGTCTCCTCCTTGGTCTACCTTTGCGAAAATTGGTATTCTCGAATGGAGATGAGGTTCATGTGATGAAGGCTCTCACAAGACTTGGCATCGAAGATTGTTTTGAGAGAATCATAAGTTTCGAGACCTTAAACCCTGATATCAACGAAGCTGAGCTTTCTTGCGTCACAGGTCATCTTCCCGAGAATCCGGTTATTTGTAAACCAACTGAGATTGCTTTCGAGAAAGCCTTCGACATTGCACAGCTCAATCCTCACAAAACCTTGTTCTTTGACGACAGTACCCGGAACATCCAAACCGGGAAAGCCGTTGGTCTCCATACAGTCTTGGTTGGTAAATCAGAGAAAATAGATGGAAGCGATTACGCGTTAGAAAGCATTCACAACATGAAAGAGGCATTCCCCGAGTTATGGTCGGAATCCATTATCAACAACAAAGAAACTGAAAGAATTGATTACGCTTCACAGATCTCGATTGAAACTTCTGTTCAAGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

266

Amino Acids

29.99

Weight (kDa)

4.67

Isoelectric Point (pI)

44.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 12 - 206 6.3e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59490 AT5G59490
malus_domestica MD03G1088500.v1.1 MD11G1097500.v1.1
prunus_persica Prupe.6G072700_v2.0.a1
pyrus_communis pycom03g07010 pycom11g08220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 180
AccI GTMKAC 1 cut(s) 322
AccII CGCG 1 cut(s) 671
AciI CCGC 1 cut(s) 215
AcoI YGGCCR 1 cut(s) 201
AcsI RAATTY 3 cut(s) 5, 171, 227
AfaI GTAC 2 cut(s) 245, 586
AfiI CCNNNNNNNGG 2 cut(s) 289, 714
AflII CTTAAG 1 cut(s) 209
AflIII ACRYGT 1 cut(s) 669
AgeI ACCGGT 1 cut(s) 249
AgsI TTSAA 5 cut(s) 128, 161, 198, 782, 794
AloI GAACNNNNNNTCC 2 cut(s) 345, 377
AluBI AGCT 7 cut(s) 18, 78, 133, 455, 460, 550, 797
AluI AGCT 7 cut(s) 18, 78, 133, 455, 460, 550, 797
Alw26I GTCTC 4 cut(s) 44, 314, 422, 623
Ama87I CYCGRG 2 cut(s) 485, 707
AoxI GGCC 1 cut(s) 201
ApoI RAATTY 3 cut(s) 5, 171, 227
AsiGI ACCGGT 1 cut(s) 249
Asp700I GAANNNNTTC 2 cut(s) 533, 681
AspS9I GGNCC 1 cut(s) 292
AsuC2I CCSGG 3 cut(s) 296, 589, 604
AvaI CYCGRG 2 cut(s) 485, 707
AvaII GGWCC 1 cut(s) 292
BaeI ACNNNNGTAYC 2 cut(s) 568, 601
BccI CCATC 2 cut(s) 143, 650
BceAI ACGGC 1 cut(s) 596
BcnI CCSGG 3 cut(s) 296, 589, 604
BcoDI GTCTC 4 cut(s) 44, 314, 422, 623
BfrI CTTAAG 1 cut(s) 209
BglII AGATCT 1 cut(s) 771
BlpI GCTNAGC 1 cut(s) 456
Bme1390I CCNGG 3 cut(s) 296, 589, 604
Bme18I GGWCC 1 cut(s) 292
BmeT110I CYCGRG 2 cut(s) 485, 707
BmgT120I GGNCC 1 cut(s) 292
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 3 cut(s) 296, 589, 604
BmsI GCATC 2 cut(s) 82, 402
Bpu1102I GCTNAGC 1 cut(s) 456
BpuMI CCSGG 3 cut(s) 296, 589, 604
BsaI GGTCTC 2 cut(s) 422, 623
BsaJI CCNNGG 1 cut(s) 316
BsaWI WCCGGW 3 cut(s) 249, 289, 493
BsaXI ACNNNNNCTCC 2 cut(s) 345, 375
Bsc4I CCNNNNNNNGG 2 cut(s) 289, 714
Bse118I RCCGGY 2 cut(s) 203, 249
Bse3DI GCAATG 1 cut(s) 540
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 2 cut(s) 61, 594
BseLI CCNNNNNNNGG 2 cut(s) 289, 714
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 2 cut(s) 447, 504
BseRI GAGGAG 2 cut(s) 32, 302
Bsh1236I CGCG 1 cut(s) 671
BshFI GGCC 1 cut(s) 203
BshTI ACCGGT 1 cut(s) 249
BsiHKCI CYCGRG 2 cut(s) 485, 707
BsiSI CCGG 7 cut(s) 204, 250, 290, 296, 494, 589, 603
BslI CCNNNNNNNGG 2 cut(s) 289, 714
BsmAI GTCTC 4 cut(s) 44, 314, 422, 623
BsmI GAATGC 2 cut(s) 681, 701
BsnI GGCC 1 cut(s) 203
Bso31I GGTCTC 2 cut(s) 422, 623
BsoBI CYCGRG 2 cut(s) 485, 707
Bsp143I GATC 1 cut(s) 771
Bsp1720I GCTNAGC 1 cut(s) 456
BspACI CCGC 1 cut(s) 215
BspANI GGCC 1 cut(s) 203
BspCNI CTCAG 2 cut(s) 448, 505
BspFNI CGCG 1 cut(s) 671
BspLI GGNNCC 1 cut(s) 294
BspTI CTTAAG 1 cut(s) 209
BspTNI GGTCTC 2 cut(s) 422, 623
BsrDI GCAATG 1 cut(s) 540
BsrFI RCCGGY 2 cut(s) 203, 249
BssAI RCCGGY 2 cut(s) 203, 249
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 1 cut(s) 771
BssT1I CCWWGG 1 cut(s) 316
Bst4CI ACNGT 2 cut(s) 584, 628
BstAFI CTTAAG 1 cut(s) 209
BstC8I GCNNGC 1 cut(s) 97
BstDEI CTNAG 3 cut(s) 302, 456, 513
BstF5I GGATG 2 cut(s) 61, 594
BstFNI CGCG 1 cut(s) 671
BstKTI GATC 1 cut(s) 774
BstMAI GTCTC 4 cut(s) 44, 314, 422, 623
BstMBI GATC 1 cut(s) 771
BstNSI RCATGY 1 cut(s) 99
BstSCI CCNGG 3 cut(s) 294, 587, 602
BstUI CGCG 1 cut(s) 671
BstX2I RGATCY 1 cut(s) 771
BstYI RGATCY 1 cut(s) 771
BsuRI GGCC 1 cut(s) 203
BtsCI GGATG 2 cut(s) 61, 594
Cac8I GCNNGC 1 cut(s) 97
Cfr10I RCCGGY 2 cut(s) 203, 249
Cfr13I GGNCC 1 cut(s) 292
CseI GACGC 1 cut(s) 457
Csp6I GTAC 2 cut(s) 244, 585
CspAI ACCGGT 1 cut(s) 249
CviAII CATG 4 cut(s) 96, 260, 365, 691
CviQI GTAC 2 cut(s) 244, 585
DdeI CTNAG 3 cut(s) 302, 456, 513
DpnI GATC 1 cut(s) 773
DpnII GATC 1 cut(s) 771
EaeI YGGCCR 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 316
Eco31I GGTCTC 2 cut(s) 422, 623
Eco32I GATATC 1 cut(s) 445
Eco47I GGWCC 1 cut(s) 292
Eco88I CYCGRG 2 cut(s) 485, 707
EcoRI GAATTC 1 cut(s) 227
EcoRV GATATC 1 cut(s) 445
EcoT14I CCWWGG 1 cut(s) 316
EcoT22I ATGCAT 1 cut(s) 97
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 4 cut(s) 99, 263, 368, 694
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FatI CATG 4 cut(s) 95, 259, 364, 690
FblI GTMKAC 1 cut(s) 322
FokI GGATG 2 cut(s) 68, 581
HaeIII GGCC 1 cut(s) 203
HapII CCGG 7 cut(s) 204, 250, 290, 296, 494, 589, 603
HgaI GACGC 1 cut(s) 457
Hin1II CATG 4 cut(s) 99, 263, 368, 694
HindIII AAGCTT 2 cut(s) 131, 795
HinfI GANTC 5 cut(s) 153, 307, 414, 490, 722
HpaII CCGG 7 cut(s) 204, 250, 290, 296, 494, 589, 603
Hpy166II GTNNAC 3 cut(s) 13, 323, 506
Hpy188I TCNGA 3 cut(s) 91, 646, 721
Hpy188III TCNNGA 5 cut(s) 346, 427, 485, 526, 775
Hpy8I GTNNAC 3 cut(s) 13, 323, 506
HpyAV CCTTC 2 cut(s) 367, 544
HpyCH4III ACNGT 2 cut(s) 584, 628
HpyCH4V TGCA 2 cut(s) 95, 545
HpyF3I CTNAG 3 cut(s) 302, 456, 513
Hsp92II CATG 4 cut(s) 99, 263, 368, 694
Kzo9I GATC 1 cut(s) 771
LmnI GCTCC 1 cut(s) 104
LweI GCATC 2 cut(s) 82, 402
MaeIII GTNAC 1 cut(s) 469
MalI GATC 1 cut(s) 773
MboI GATC 1 cut(s) 771
MboII GAAGA 3 cut(s) 158, 410, 473
MflI RGATCY 1 cut(s) 771
MluCI AATT 5 cut(s) 5, 171, 227, 335, 753
MluI ACGCGT 1 cut(s) 669
MlyI GAGTC 2 cut(s) 162, 316
MmeI TCCRAC 2 cut(s) 126, 699
MnlI CCTC 5 cut(s) 53, 323, 352, 567, 691
Mph1103I ATGCAT 1 cut(s) 97
MroXI GAANNNNTTC 2 cut(s) 533, 681
MseI TTAA 5 cut(s) 74, 210, 285, 434, 799
MslI CAYNNNNRTG 1 cut(s) 252
MspCI CTTAAG 1 cut(s) 209
MspI CCGG 7 cut(s) 204, 250, 290, 296, 494, 589, 603
MspR9I CCNGG 3 cut(s) 296, 589, 604
Mva1269I GAATGC 2 cut(s) 681, 701
MvnI CGCG 1 cut(s) 671
NciI CCSGG 3 cut(s) 296, 589, 604
NdeII GATC 1 cut(s) 771
NlaIII CATG 4 cut(s) 99, 263, 368, 694
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 469
NsiI ATGCAT 1 cut(s) 97
NspI RCATGY 1 cut(s) 99
PaeI GCATGC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 237
PctI GAATGC 2 cut(s) 681, 701
PdmI GAANNNNTTC 2 cut(s) 533, 681
PfeI GAWTC 3 cut(s) 414, 490, 722
PinAI ACCGGT 1 cut(s) 249
PleI GAGTC 2 cut(s) 161, 315
PpsI GAGTC 2 cut(s) 161, 315
PsiI TTATAA 1 cut(s) 180
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 292
PsuI RGATCY 1 cut(s) 771
RsaI GTAC 2 cut(s) 245, 586
RsaNI GTAC 2 cut(s) 244, 585
RseI CAYNNNNRTG 1 cut(s) 252
SaqAI TTAA 5 cut(s) 74, 210, 285, 434, 799
Sau3AI GATC 1 cut(s) 771
Sau96I GGNCC 1 cut(s) 292
SchI GAGTC 2 cut(s) 162, 316
ScrFI CCNGG 3 cut(s) 296, 589, 604
SfaNI GCATC 2 cut(s) 82, 402
SinI GGWCC 1 cut(s) 292
SmiMI CAYNNNNRTG 1 cut(s) 252
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
SphI GCATGC 1 cut(s) 99
Sse9I AATT 5 cut(s) 5, 171, 227, 335, 753
SsiI CCGC 1 cut(s) 215
StyD4I CCNGG 3 cut(s) 294, 587, 602
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 2 cut(s) 584, 628
TaqI TCGA 7 cut(s) 231, 347, 396, 426, 525, 537, 776
TasI AATT 5 cut(s) 5, 171, 227, 335, 753
TfiI GAWTC 3 cut(s) 414, 490, 722
Tru1I TTAA 5 cut(s) 74, 210, 285, 434, 799
Tru9I TTAA 5 cut(s) 74, 210, 285, 434, 799
TseFI GTSAC 1 cut(s) 469
Tsp45I GTSAC 1 cut(s) 469
TspDTI ATGAA 8 cut(s) 48, 159, 240, 248, 291, 353, 386, 707
Vha464I CTTAAG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 292
XapI RAATTY 3 cut(s) 5, 171, 227
XceI RCATGY 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 322
XmnI GAANNNNTTC 2 cut(s) 533, 681
Zsp2I ATGCAT 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.