MD03G1088500.v1.1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0019565
Physical Location & Seq
Forward (+)
7310266 .. 7312362
2097 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1088500.v1.1.491

Sequence Viewer

Length: 849 bp
ATGGAATTCCAAAACCAGTTTCAGGAGGCGGACCAGATGCCAAAATATGAGTGCCTTCTCTTTGACCTTGATGATACCCTTTATCCCTTGAGTTCTGGATTGTCTGATCAATGCACCAAGAATATTGAAGAATTCATGGTTCAAAAATTAGGCATCGAGGAAAAAAGAGTCACTGAGGTGAATCAAGTATTGTACAAGAATTATGGAACATCAATAGCTGGTCTCAAGGCCATTGGTTATAATTTTGACAACGAGGACTATCATAGTTTTGTTCATGGAAGATTGCCCTATGAGAATCTAAGACCTGACCCTGTCCTAAGGACTCTATTGCTGAGCCTGCCATACAGGAAACTTATTTTCTCAAATGGAAATAACGTCCACGTGGCAAAAACCCTAAGCAAGCTTGGATTGGATGACTGCTTTGAAGGAGTTATTAGCTTTGAGACTCTGAATCCCACTAAAGATGATGAAGGATGCAGCACAAGTGTTTTTGATCGTTCTTGTTTATTTGATGCTGGAATATCAGAGCTTCCAGCGACTCCTATTGTTTGCAAACCATTTGCAAATGCATATGAACAAGCCTTTAAAAAAGCCAACATTAACCCTCAAAGAACTATATTCTTTGATGATAGCCTGAGGAACATACAAACTGGAAAGAACATGGGTCTCCATACTGTATTGGTGGGCAGCTCCAACAGAACAAAGGGTGTGGATTATGCAATAGAGAGCATCCACAATATCAGAGAAGCACTTCCAGAACTTTGGGAAATCGACAATAAAAATACAGACACGGCTAGAACTTTGTCAGGGAAGCTGGCAATGGAGAAATCAGTGACTGCCGAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

283

Amino Acids

31.76

Weight (kDa)

5.01

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 17 - 222 1e-10 haloacid dehalogenase-like hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59490 AT5G59490
malus_domestica MD03G1088500.v1.1 MD11G1097500.v1.1
prunus_persica Prupe.6G072700_v2.0.a1
pyrus_communis pycom03g07010 pycom11g08220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AciI CCGC 1 cut(s) 29
AcsI RAATTY 2 cut(s) 5, 131
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 3 cut(s) 128, 143, 425
AjuI GAANNNNNNNTTGG 1 cut(s) 35
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 6 cut(s) 218, 403, 438, 529, 690, 814
AluI AGCT 6 cut(s) 218, 403, 438, 529, 690, 814
Alw26I GTCTC 3 cut(s) 227, 437, 671
AlwNI CAGNNNCTG 1 cut(s) 836
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 477, 687
ApoI RAATTY 2 cut(s) 5, 131
Asp700I GAANNNNTTC 1 cut(s) 750
AspS9I GGNCC 1 cut(s) 31
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 31
AxyI CCTNAGG 2 cut(s) 317, 635
BbrPI CACGTG 1 cut(s) 382
BbvI GCAGC 2 cut(s) 489, 699
BceAI ACGGC 1 cut(s) 807
BclI TGATCA 1 cut(s) 106
BcoDI GTCTC 3 cut(s) 227, 437, 671
BfaI CTAG 1 cut(s) 795
BisI GCNGC 2 cut(s) 478, 688
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 479, 689
Bme18I GGWCC 1 cut(s) 31
BmgT120I GGNCC 1 cut(s) 31
BmsI GCATC 5 cut(s) 27, 162, 464, 502, 738
Bpu10I CCTNAGC 1 cut(s) 395
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 109, 209
BsaAI YACGTR 1 cut(s) 382
BsaI GGTCTC 2 cut(s) 227, 671
BsaJI CCNNGG 1 cut(s) 840
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 2 cut(s) 16, 655
Bse21I CCTNAGG 2 cut(s) 317, 635
Bse3DI GCAATG 1 cut(s) 825
BseDI CCNNGG 1 cut(s) 840
BseGI GGATG 3 cut(s) 418, 479, 729
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 1 cut(s) 825
BseMII CTCAG 3 cut(s) 165, 323, 626
BseNI ACTGG 2 cut(s) 16, 655
BseXI GCAGC 2 cut(s) 489, 699
BshFI GGCC 1 cut(s) 230
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 3 cut(s) 227, 437, 671
BsnI GGCC 1 cut(s) 230
Bso31I GGTCTC 2 cut(s) 227, 671
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 2 cut(s) 106, 493
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 1 cut(s) 29
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 3 cut(s) 166, 324, 627
BspTNI GGTCTC 2 cut(s) 227, 671
BsrDI GCAATG 1 cut(s) 825
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 2 cut(s) 16, 655
BssECI CCNNGG 1 cut(s) 840
BssMI GATC 2 cut(s) 106, 493
Bst4CI ACNGT 1 cut(s) 676
BstAUI TGTACA 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 3 cut(s) 338, 401, 816
BstDEI CTNAG 7 cut(s) 174, 299, 317, 332, 395, 635, 846
BstF5I GGATG 3 cut(s) 418, 479, 729
BstKTI GATC 2 cut(s) 109, 496
BstMAI GTCTC 3 cut(s) 227, 437, 671
BstMBI GATC 2 cut(s) 106, 493
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstV1I GCAGC 2 cut(s) 489, 699
BstXI CCANNNNNNTGG 1 cut(s) 762
Bsu36I CCTNAGG 2 cut(s) 317, 635
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 3 cut(s) 418, 479, 729
BtsIMutI CAGTG 2 cut(s) 171, 837
Cac8I GCNNGC 3 cut(s) 338, 401, 816
CaiI CAGNNNCTG 1 cut(s) 836
Cfr13I GGNCC 1 cut(s) 31
Csp6I GTAC 1 cut(s) 193
CspCI CAANNNNNGTGG 2 cut(s) 690, 725
CviAII CATG 3 cut(s) 136, 275, 661
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 7 cut(s) 174, 299, 317, 332, 395, 635, 846
DpnI GATC 2 cut(s) 108, 495
DpnII GATC 2 cut(s) 106, 493
DraI TTTAAA 1 cut(s) 586
EciI GGCGGA 1 cut(s) 44
Eco31I GGTCTC 2 cut(s) 227, 671
Eco47I GGWCC 1 cut(s) 31
Eco72I CACGTG 1 cut(s) 382
Eco81I CCTNAGG 2 cut(s) 317, 635
EcoRI GAATTC 2 cut(s) 5, 131
EcoT22I ATGCAT 1 cut(s) 571
FaeI CATG 3 cut(s) 139, 278, 664
FatI CATG 3 cut(s) 135, 274, 660
FauNDI CATATG 1 cut(s) 571
FbaI TGATCA 1 cut(s) 106
Fnu4HI GCNGC 2 cut(s) 478, 688
FokI GGATG 3 cut(s) 425, 486, 716
Fsp4HI GCNGC 2 cut(s) 478, 688
FspBI CTAG 1 cut(s) 795
GluI GCNGC 2 cut(s) 478, 688
HaeIII GGCC 1 cut(s) 230
Hin1II CATG 3 cut(s) 139, 278, 664
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 7 cut(s) 168, 181, 295, 322, 445, 451, 538
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 106, 450, 526, 743
Hpy188III TCNNGA 3 cut(s) 23, 96, 755
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 3 cut(s) 65, 419, 464
HpyCH4III ACNGT 1 cut(s) 676
HpyCH4IV ACGT 2 cut(s) 375, 381
HpyCH4V TGCA 6 cut(s) 114, 477, 552, 563, 569, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 7 cut(s) 174, 299, 317, 332, 395, 635, 846
HpySE526I ACGT 2 cut(s) 375, 381
Hsp92II CATG 3 cut(s) 139, 278, 664
Ksp22I TGATCA 1 cut(s) 106
Kzo9I GATC 2 cut(s) 106, 493
LmnI GCTCC 1 cut(s) 695
Lsp1109I GCAGC 2 cut(s) 489, 699
LweI GCATC 5 cut(s) 27, 162, 464, 502, 738
MaeI CTAG 1 cut(s) 795
MaeII ACGT 2 cut(s) 375, 381
MaeIII GTNAC 2 cut(s) 169, 832
MalI GATC 2 cut(s) 108, 495
MboI GATC 2 cut(s) 106, 493
MboII GAAGA 2 cut(s) 140, 291
MluCI AATT 5 cut(s) 5, 131, 146, 199, 241
MlyI GAGTC 4 cut(s) 177, 316, 439, 532
MmeI TCCRAC 1 cut(s) 717
MnlI CCTC 7 cut(s) 19, 151, 169, 247, 615, 630, 835
Mph1103I ATGCAT 1 cut(s) 571
MroXI GAANNNNTTC 1 cut(s) 750
MseI TTAA 2 cut(s) 585, 600
MslI CAYNNNNRTG 1 cut(s) 176
MwoI GCNNNNNNNGC 1 cut(s) 337
NdeI CATATG 1 cut(s) 571
NdeII GATC 2 cut(s) 106, 493
NlaIII CATG 3 cut(s) 139, 278, 664
NmuCI GTSAC 2 cut(s) 169, 832
NsiI ATGCAT 1 cut(s) 571
OliI CACNNNNGTG 1 cut(s) 176
PdmI GAANNNNTTC 1 cut(s) 750
PfeI GAWTC 3 cut(s) 181, 295, 451
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 479, 689
PleI GAGTC 4 cut(s) 176, 316, 439, 532
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 4 cut(s) 176, 316, 439, 532
Ppu21I YACGTR 1 cut(s) 382
PsiI TTATAA 1 cut(s) 240
PspCI CACGTG 1 cut(s) 382
PspPI GGNCC 1 cut(s) 31
PstNI CAGNNNCTG 1 cut(s) 836
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 1 cut(s) 176
SaqAI TTAA 2 cut(s) 585, 600
SatI GCNGC 2 cut(s) 478, 688
Sau3AI GATC 2 cut(s) 106, 493
Sau96I GGNCC 1 cut(s) 31
SchI GAGTC 4 cut(s) 177, 316, 439, 532
SfaNI GCATC 5 cut(s) 27, 162, 464, 502, 738
SinI GGWCC 1 cut(s) 31
SmiMI CAYNNNNRTG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 88, 224
SmoI CTYRAG 2 cut(s) 88, 224
Sse9I AATT 5 cut(s) 5, 131, 146, 199, 241
SsiI CCGC 1 cut(s) 29
SspI AATATT 1 cut(s) 124
SspMI CTAG 1 cut(s) 795
TaaI ACNGT 1 cut(s) 676
TaiI ACGT 2 cut(s) 378, 384
TaqI TCGA 2 cut(s) 156, 771
TasI AATT 5 cut(s) 5, 131, 146, 199, 241
TatI WGTACW 1 cut(s) 192
TfiI GAWTC 3 cut(s) 181, 295, 451
Tru1I TTAA 2 cut(s) 585, 600
Tru9I TTAA 2 cut(s) 585, 600
TscAI CASTG 2 cut(s) 178, 837
TseFI GTSAC 2 cut(s) 169, 832
TseI GCWGC 2 cut(s) 477, 687
Tsp45I GTSAC 2 cut(s) 169, 832
TspDTI ATGAA 4 cut(s) 124, 263, 483, 588
TspRI CASTG 2 cut(s) 178, 837
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 1 cut(s) 31
XapI RAATTY 2 cut(s) 5, 131
XmnI GAANNNNTTC 1 cut(s) 750
XspI CTAG 1 cut(s) 795
Zsp2I ATGCAT 1 cut(s) 571
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.