pycom11g08220

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0019565
Physical Location & Seq
Forward (+)
6340262 .. 6342082
1821 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g08220.1

Sequence Viewer

Length: 849 bp
ATGGAATTCCAAAACCAGTATCAGCAGGCTGATCAGATGCCAGAATACGAGTGCCTTCTCTTTGATCTTGATGACACCCTTTATCCCTTGAGTTCTGGATTATCTAAACAATGCACCAAGAACATTGAAGAATTCATGGTTAAAAAATTAGGCATAGAGGAAAAAAGAGTCACTGAGGTGAATCAAGTATTGTACAAGAATTATGGAACATCAATGGCCGGTCTCAAGGCTATTGGTTACAATTTTGACAATGATGACTATCATAGTTTTGTTCATGGAAGATTGCCCTATGAGAATCTAAGACCTGACCATGTCTTAAGGACTCTATTGCTAAGCCTGCCATACAGGAAACTTATTTTCTCAAATGGGGATAAGGTCCACGTGGCAAAAACCCTAAGCAAGCTTGGATTGAATGACTGCTTTGAAGGAGTTATTTGCTTTGAGACTCTGAATCCCACTAATGATGATGAAGGATGCAGCAGCACAAGTATTTTTTATCATTCTTGCTTATTTGATGCTGAAATATCAGAGCTTCCAGTGACTCCAATTGTTTGCAAGCCATTTGCAAATGCATATGAACAAGCCTTTGAAAAAGCCAACATTAACCCTCAAAGAACTCTATTCTTTGATGATAGCATCAGGAACATACAAGCTGGAAAGAACATCGGTCTCCATACTGTATTGGTTGGCACTTCCAACAGAACCAAGGGTGTGGATTATGCAATCGAGAGCATCCACAATATCAGAGAAGCACTTCCAGAACTATGGGAAATCAACAATAAGGACACAGCCATGGGTAGAACCTTTTCAGGGAAGCTGCTGGCAATTAAGACATCAGTGACCGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

283

Amino Acids

31.92

Weight (kDa)

5.32

Isoelectric Point (pI)

43.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hydrolase PF00702 17 - 223 4.1e-11 haloacid dehalogenase-like hydrolase
HAD_2 PF13419 186 - 229 6.6e-06 Haloacid dehalogenase-like hydrolase
Hydrolase_like PF13242 186 - 236 5.2e-06 HAD-hyrolase-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59490 AT5G59490
malus_domestica MD03G1088500.v1.1 MD11G1097500.v1.1
prunus_persica Prupe.6G072700_v2.0.a1
pyrus_communis pycom03g07010 pycom11g08220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 843
AcoI YGGCCR 1 cut(s) 216
AcsI RAATTY 2 cut(s) 5, 131
AcvI CACGTG 1 cut(s) 382
AfaI GTAC 1 cut(s) 194
AfiI CCNNNNNNNGG 1 cut(s) 810
AflII CTTAAG 1 cut(s) 316
AgsI TTSAA 4 cut(s) 128, 412, 425, 590
AleI CACNNNNGTG 1 cut(s) 176
AluBI AGCT 4 cut(s) 403, 532, 653, 817
AluI AGCT 4 cut(s) 403, 532, 653, 817
Alw26I GTCTC 3 cut(s) 227, 437, 674
AoxI GGCC 1 cut(s) 216
ApeKI GCWGC 3 cut(s) 477, 480, 817
ApoI RAATTY 2 cut(s) 5, 131
Asp700I GAANNNNTTC 2 cut(s) 753, 805
AspS9I GGNCC 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 190
AvaII GGWCC 1 cut(s) 376
BbrPI CACGTG 1 cut(s) 382
BbvI GCAGC 3 cut(s) 489, 492, 804
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 3 cut(s) 227, 437, 674
BfaI CTAG 1 cut(s) 847
BfrI CTTAAG 1 cut(s) 316
BisI GCNGC 3 cut(s) 478, 481, 818
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 3 cut(s) 479, 482, 819
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 376
BmsI GCATC 5 cut(s) 27, 464, 505, 645, 741
Bpu10I CCTNAGC 1 cut(s) 395
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 2 cut(s) 109, 209
BsaAI YACGTR 1 cut(s) 382
BsaBI GATNNNNATC 1 cut(s) 258
BsaI GGTCTC 2 cut(s) 227, 674
BsaJI CCNNGG 2 cut(s) 705, 792
Bsc4I CCNNNNNNNGG 1 cut(s) 810
Bse118I RCCGGY 1 cut(s) 218
Bse1I ACTGG 2 cut(s) 16, 536
Bse8I GATNNNNATC 1 cut(s) 258
BseDI CCNNGG 2 cut(s) 705, 792
BseGI GGATG 2 cut(s) 479, 732
BseJI GATNNNNATC 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 810
BseMII CTCAG 1 cut(s) 165
BseNI ACTGG 2 cut(s) 16, 536
BseXI GCAGC 3 cut(s) 489, 492, 804
BshFI GGCC 1 cut(s) 218
BsiSI CCGG 1 cut(s) 219
BslI CCNNNNNNNGG 1 cut(s) 810
BsmAI GTCTC 3 cut(s) 227, 437, 674
BsnI GGCC 1 cut(s) 218
Bso31I GGTCTC 2 cut(s) 227, 674
Bsp1407I TGTACA 1 cut(s) 192
Bsp143I GATC 2 cut(s) 31, 64
Bsp1720I GCTNAGC 1 cut(s) 332
Bsp19I CCATGG 1 cut(s) 792
BspACI CCGC 1 cut(s) 843
BspANI GGCC 1 cut(s) 218
BspCNI CTCAG 1 cut(s) 166
BspTI CTTAAG 1 cut(s) 316
BspTNI GGTCTC 2 cut(s) 227, 674
BsrFI RCCGGY 1 cut(s) 218
BsrGI TGTACA 1 cut(s) 192
BsrI ACTGG 2 cut(s) 16, 536
BssAI RCCGGY 1 cut(s) 218
BssECI CCNNGG 2 cut(s) 705, 792
BssMI GATC 2 cut(s) 31, 64
BssT1I CCWWGG 2 cut(s) 705, 792
Bst4CI ACNGT 1 cut(s) 679
BstAFI CTTAAG 1 cut(s) 316
BstAUI TGTACA 1 cut(s) 192
BstBAI YACGTR 1 cut(s) 382
BstC8I GCNNGC 5 cut(s) 27, 338, 401, 557, 822
BstDEI CTNAG 4 cut(s) 174, 299, 332, 395
BstDSI CCRYGG 1 cut(s) 792
BstF5I GGATG 2 cut(s) 479, 732
BstKTI GATC 2 cut(s) 34, 67
BstMAI GTCTC 3 cut(s) 227, 437, 674
BstMBI GATC 2 cut(s) 31, 64
BstMWI GCNNNNNNNGC 1 cut(s) 337
BstV1I GCAGC 3 cut(s) 489, 492, 804
BstXI CCANNNNNNTGG 2 cut(s) 712, 765
BsuRI GGCC 1 cut(s) 218
BtgI CCRYGG 1 cut(s) 792
BtsCI GGATG 2 cut(s) 479, 732
BtsIMutI CAGTG 3 cut(s) 171, 543, 843
Cac8I GCNNGC 5 cut(s) 27, 338, 401, 557, 822
Cfr10I RCCGGY 1 cut(s) 218
Cfr13I GGNCC 1 cut(s) 376
Csp6I GTAC 1 cut(s) 193
CviAII CATG 4 cut(s) 136, 275, 311, 793
CviQI GTAC 1 cut(s) 193
DdeI CTNAG 4 cut(s) 174, 299, 332, 395
DpnI GATC 2 cut(s) 33, 66
DpnII GATC 2 cut(s) 31, 64
EaeI YGGCCR 1 cut(s) 216
Eco130I CCWWGG 2 cut(s) 705, 792
Eco31I GGTCTC 2 cut(s) 227, 674
Eco47I GGWCC 1 cut(s) 376
Eco72I CACGTG 1 cut(s) 382
EcoRI GAATTC 2 cut(s) 5, 131
EcoT14I CCWWGG 2 cut(s) 705, 792
EcoT22I ATGCAT 1 cut(s) 574
ErhI CCWWGG 2 cut(s) 705, 792
FaeI CATG 4 cut(s) 139, 278, 314, 796
FatI CATG 4 cut(s) 135, 274, 310, 792
FauNDI CATATG 1 cut(s) 574
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 3 cut(s) 478, 481, 818
FokI GGATG 2 cut(s) 486, 719
Fsp4HI GCNGC 3 cut(s) 478, 481, 818
FspBI CTAG 1 cut(s) 847
GluI GCNGC 3 cut(s) 478, 481, 818
HaeIII GGCC 1 cut(s) 218
HapII CCGG 1 cut(s) 219
Hin1II CATG 4 cut(s) 139, 278, 314, 796
HindIII AAGCTT 1 cut(s) 401
HinfI GANTC 7 cut(s) 168, 181, 295, 322, 445, 451, 541
HpaII CCGG 1 cut(s) 219
HphI GGTGA 1 cut(s) 190
Hpy166II GTNNAC 1 cut(s) 379
Hpy188I TCNGA 4 cut(s) 36, 450, 529, 746
Hpy188III TCNNGA 5 cut(s) 68, 96, 640, 727, 758
Hpy8I GTNNAC 1 cut(s) 379
HpyAV CCTTC 3 cut(s) 65, 419, 464
HpyCH4III ACNGT 1 cut(s) 679
HpyCH4IV ACGT 1 cut(s) 381
HpyCH4V TGCA 6 cut(s) 114, 477, 555, 566, 572, 722
HpyF10VI GCNNNNNNNGC 1 cut(s) 337
HpyF3I CTNAG 4 cut(s) 174, 299, 332, 395
HpySE526I ACGT 1 cut(s) 381
Hsp92II CATG 4 cut(s) 139, 278, 314, 796
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 2 cut(s) 31, 64
Lsp1109I GCAGC 3 cut(s) 489, 492, 804
LweI GCATC 5 cut(s) 27, 464, 505, 645, 741
MaeI CTAG 1 cut(s) 847
MaeII ACGT 1 cut(s) 381
MaeIII GTNAC 4 cut(s) 169, 236, 538, 838
MalI GATC 2 cut(s) 33, 66
MboI GATC 2 cut(s) 31, 64
MboII GAAGA 2 cut(s) 140, 291
MfeI CAATTG 1 cut(s) 546
MluCI AATT 7 cut(s) 5, 131, 146, 199, 241, 546, 825
MlyI GAGTC 4 cut(s) 177, 316, 439, 535
MmeI TCCRAC 1 cut(s) 720
MnlI CCTC 3 cut(s) 151, 169, 618
Mph1103I ATGCAT 1 cut(s) 574
MroXI GAANNNNTTC 2 cut(s) 753, 805
MseI TTAA 4 cut(s) 141, 317, 603, 828
MslI CAYNNNNRTG 2 cut(s) 176, 791
MspCI CTTAAG 1 cut(s) 316
MspI CCGG 1 cut(s) 219
MunI CAATTG 1 cut(s) 546
MwoI GCNNNNNNNGC 1 cut(s) 337
NcoI CCATGG 1 cut(s) 792
NdeI CATATG 1 cut(s) 574
NdeII GATC 2 cut(s) 31, 64
NlaIII CATG 4 cut(s) 139, 278, 314, 796
NmuCI GTSAC 3 cut(s) 169, 538, 838
NsiI ATGCAT 1 cut(s) 574
OliI CACNNNNGTG 1 cut(s) 176
PdmI GAANNNNTTC 2 cut(s) 753, 805
PfeI GAWTC 3 cut(s) 181, 295, 451
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 3 cut(s) 479, 482, 819
PleI GAGTC 4 cut(s) 176, 316, 439, 535
PmaCI CACGTG 1 cut(s) 382
PmlI CACGTG 1 cut(s) 382
PpsI GAGTC 4 cut(s) 176, 316, 439, 535
Ppu21I YACGTR 1 cut(s) 382
PspCI CACGTG 1 cut(s) 382
PspPI GGNCC 1 cut(s) 376
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 1 cut(s) 194
RsaNI GTAC 1 cut(s) 193
RseI CAYNNNNRTG 2 cut(s) 176, 791
SaqAI TTAA 4 cut(s) 141, 317, 603, 828
SatI GCNGC 3 cut(s) 478, 481, 818
Sau3AI GATC 2 cut(s) 31, 64
Sau96I GGNCC 1 cut(s) 376
SchI GAGTC 4 cut(s) 177, 316, 439, 535
SetI ASST 9 cut(s) 180, 307, 378, 384, 405, 534, 655, 806, 819
SfaNI GCATC 5 cut(s) 27, 464, 505, 645, 741
SinI GGWCC 1 cut(s) 376
SmiMI CAYNNNNRTG 2 cut(s) 176, 791
SmlI CTYRAG 3 cut(s) 88, 224, 316
SmoI CTYRAG 3 cut(s) 88, 224, 316
Sse9I AATT 7 cut(s) 5, 131, 146, 199, 241, 546, 825
SsiI CCGC 1 cut(s) 843
SspMI CTAG 1 cut(s) 847
StyI CCWWGG 2 cut(s) 705, 792
TaaI ACNGT 1 cut(s) 679
TaiI ACGT 1 cut(s) 384
TaqI TCGA 1 cut(s) 726
TaqII GACCGA 1 cut(s) 656
TasI AATT 7 cut(s) 5, 131, 146, 199, 241, 546, 825
TatI WGTACW 1 cut(s) 192
TfiI GAWTC 3 cut(s) 181, 295, 451
Tru1I TTAA 4 cut(s) 141, 317, 603, 828
Tru9I TTAA 4 cut(s) 141, 317, 603, 828
TscAI CASTG 3 cut(s) 178, 543, 843
TseFI GTSAC 3 cut(s) 169, 538, 838
TseI GCWGC 3 cut(s) 477, 480, 817
Tsp45I GTSAC 3 cut(s) 169, 538, 838
TspDTI ATGAA 4 cut(s) 124, 263, 483, 591
TspRI CASTG 3 cut(s) 178, 543, 843
Tth111I GACNNNGTC 1 cut(s) 311
Vha464I CTTAAG 1 cut(s) 316
VpaK11BI GGWCC 1 cut(s) 376
XapI RAATTY 2 cut(s) 5, 131
XmnI GAANNNNTTC 2 cut(s) 753, 805
XspI CTAG 1 cut(s) 847
Zsp2I ATGCAT 1 cut(s) 574
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.