pycom03g07010

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0019565
Physical Location & Seq
Forward (+)
5571762 .. 5573642
1881 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g07010.5

Sequence Viewer

Length: 846 bp
ATGGAATTCCAAAACCAATATCAGGAGGCTGATCAGATGAAATATGAGTGCCTTCTCTTTGACGTTGATGATACCCTTTATCCCTTGAGTTCTGGATTGTCTGATCAATGCACCAAGAATATTGAAGAATTCATGGTTCAAAAATTAGGCATCGAGGAAAAAAGAGTCGCTGAGTTGAATCAAGTACTGTACAAGAATTATGGAACATCAATAGCTGGTCTCAAGGCCATTGGTTATAATTTTGATAACGAGGACTATCATAGTTTTGTTCATGGAAGATTGCCCTATGAGAATCTAAGACCTGACCCTGTCCTAAGGGCTCTATTGCTGAGCCTGCCATATAGGAAACTTATTTTCTCTAATGCGGATAACGTCCACGTGGCAAAAACCCTAAGCAAGCTTGGATTGGATGACTGCTTTGAAGGAGTTATTAGCTTTGAGACTCTGAATCCCACTAAAGATGATGAAGGATGCAGCACAAGTGTTTTTTATCGTTCTTGTTTATTTGATGCTGGAATATCAGAGCTTCCGGCGACTCCAATTGTTTGCAAACCATTTGCAAATGCATATGAACAAGCCCTTAAAAATGCCAACATTAACCCTCAAAGAACTATATTCTTTGATGATAGCCTAAGGAACATACAAACTGGAAAGAACATGGGTCTCCATACTGTATTGGTGGGCAACTCCAACAGAACAAAGGGTGTGGATTATGCAATAGAGAGCATCCACAATATCAGAGAAGCACTTCCAGAACTTTGGGAAATCGACAATAAAAATACAGACACGGCTAGAACTTTGTCAGGGGAGCTGGCAATGGAGAAATCAGTGACTGCCAGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

282

Amino Acids

31.69

Weight (kDa)

4.93

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59490 AT5G59490
malus_domestica MD03G1088500.v1.1 MD11G1097500.v1.1
prunus_persica Prupe.6G072700_v2.0.a1
pyrus_communis pycom03g07010 pycom11g08220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 237
AciI CCGC 1 cut(s) 365
AcsI RAATTY 2 cut(s) 5, 128
AcvI CACGTG 1 cut(s) 379
AfaI GTAC 2 cut(s) 186, 191
AfiI CCNNNNNNNGG 1 cut(s) 22
AgsI TTSAA 4 cut(s) 125, 140, 178, 422
AjnI CCWGG 1 cut(s) 836
AluBI AGCT 5 cut(s) 215, 400, 435, 526, 811
AluI AGCT 5 cut(s) 215, 400, 435, 526, 811
Alw26I GTCTC 3 cut(s) 224, 434, 668
AlwNI CAGNNNCTG 1 cut(s) 833
AoxI GGCC 1 cut(s) 225
ApeKI GCWGC 1 cut(s) 474
ApoI RAATTY 2 cut(s) 5, 128
Asp700I GAANNNNTTC 1 cut(s) 747
AxyI CCTNAGG 2 cut(s) 314, 632
BanII GRGCYC 1 cut(s) 322
BbrPI CACGTG 1 cut(s) 379
BbvI GCAGC 1 cut(s) 486
BceAI ACGGC 1 cut(s) 804
BciT130I CCWGG 1 cut(s) 838
BclI TGATCA 2 cut(s) 31, 103
BcoDI GTCTC 3 cut(s) 224, 434, 668
BfaI CTAG 1 cut(s) 792
BisI GCNGC 1 cut(s) 475
BlpI GCTNAGC 1 cut(s) 329
BlsI GCNGC 1 cut(s) 476
BmcAI AGTACT 1 cut(s) 186
Bme1390I CCNGG 1 cut(s) 838
BmrFI CCNGG 1 cut(s) 838
BmsI GCATC 4 cut(s) 159, 461, 499, 735
Bpu10I CCTNAGC 1 cut(s) 392
Bpu1102I GCTNAGC 1 cut(s) 329
BpuEI CTTGAG 2 cut(s) 106, 206
BsaAI YACGTR 1 cut(s) 379
BsaI GGTCTC 2 cut(s) 224, 668
BsaJI CCNNGG 1 cut(s) 837
Bsc4I CCNNNNNNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 652
Bse21I CCTNAGG 2 cut(s) 314, 632
Bse3DI GCAATG 1 cut(s) 822
BseBI CCWGG 1 cut(s) 838
BseDI CCNNGG 1 cut(s) 837
BseGI GGATG 3 cut(s) 415, 476, 726
BseLI CCNNNNNNNGG 1 cut(s) 22
BseMI GCAATG 1 cut(s) 822
BseMII CTCAG 2 cut(s) 162, 320
BseNI ACTGG 1 cut(s) 652
BseXI GCAGC 1 cut(s) 486
BshFI GGCC 1 cut(s) 227
BsiSI CCGG 1 cut(s) 530
BslI CCNNNNNNNGG 1 cut(s) 22
BsmAI GTCTC 3 cut(s) 224, 434, 668
BsnI GGCC 1 cut(s) 227
Bso31I GGTCTC 2 cut(s) 224, 668
Bsp1286I GDGCHC 1 cut(s) 322
Bsp1407I TGTACA 1 cut(s) 189
Bsp143I GATC 2 cut(s) 31, 103
Bsp1720I GCTNAGC 1 cut(s) 329
BspACI CCGC 1 cut(s) 365
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 2 cut(s) 163, 321
BspTNI GGTCTC 2 cut(s) 224, 668
BsrDI GCAATG 1 cut(s) 822
BsrGI TGTACA 1 cut(s) 189
BsrI ACTGG 1 cut(s) 652
BssECI CCNNGG 1 cut(s) 837
BssMI GATC 2 cut(s) 31, 103
Bst2UI CCWGG 1 cut(s) 838
Bst4CI ACNGT 2 cut(s) 189, 673
BstAUI TGTACA 1 cut(s) 189
BstBAI YACGTR 1 cut(s) 379
BstC8I GCNNGC 3 cut(s) 335, 398, 813
BstDEI CTNAG 7 cut(s) 171, 296, 314, 329, 392, 632, 843
BstF5I GGATG 3 cut(s) 415, 476, 726
BstKTI GATC 2 cut(s) 34, 106
BstMAI GTCTC 3 cut(s) 224, 434, 668
BstMBI GATC 2 cut(s) 31, 103
BstMWI GCNNNNNNNGC 1 cut(s) 334
BstNI CCWGG 1 cut(s) 838
BstSCI CCNGG 1 cut(s) 836
BstV1I GCAGC 1 cut(s) 486
BstXI CCANNNNNNTGG 1 cut(s) 759
Bsu36I CCTNAGG 2 cut(s) 314, 632
BsuRI GGCC 1 cut(s) 227
BtsCI GGATG 3 cut(s) 415, 476, 726
BtsIMutI CAGTG 1 cut(s) 834
Cac8I GCNNGC 3 cut(s) 335, 398, 813
CaiI CAGNNNCTG 1 cut(s) 833
Csp6I GTAC 2 cut(s) 185, 190
CspCI CAANNNNNGTGG 2 cut(s) 687, 722
CviAII CATG 3 cut(s) 133, 272, 658
CviQI GTAC 2 cut(s) 185, 190
DdeI CTNAG 7 cut(s) 171, 296, 314, 329, 392, 632, 843
DpnI GATC 2 cut(s) 33, 105
DpnII GATC 2 cut(s) 31, 103
Eco24I GRGCYC 1 cut(s) 322
Eco31I GGTCTC 2 cut(s) 224, 668
Eco72I CACGTG 1 cut(s) 379
Eco81I CCTNAGG 2 cut(s) 314, 632
EcoRI GAATTC 2 cut(s) 5, 128
EcoRII CCWGG 1 cut(s) 836
EcoT22I ATGCAT 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 322
FaeI CATG 3 cut(s) 136, 275, 661
FatI CATG 3 cut(s) 132, 271, 657
FauNDI CATATG 1 cut(s) 568
FbaI TGATCA 2 cut(s) 31, 103
Fnu4HI GCNGC 1 cut(s) 475
FokI GGATG 3 cut(s) 422, 483, 713
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 475
FspBI CTAG 1 cut(s) 792
GluI GCNGC 1 cut(s) 475
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 530
Hin1II CATG 3 cut(s) 136, 275, 661
HindIII AAGCTT 1 cut(s) 398
HinfI GANTC 6 cut(s) 165, 178, 292, 442, 448, 535
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 376
Hpy188I TCNGA 5 cut(s) 36, 103, 447, 523, 740
Hpy188III TCNNGA 3 cut(s) 23, 93, 752
Hpy8I GTNNAC 1 cut(s) 376
HpyAV CCTTC 3 cut(s) 62, 416, 461
HpyCH4III ACNGT 2 cut(s) 189, 673
HpyCH4IV ACGT 3 cut(s) 63, 372, 378
HpyCH4V TGCA 6 cut(s) 111, 474, 549, 560, 566, 716
HpyF10VI GCNNNNNNNGC 1 cut(s) 334
HpyF3I CTNAG 7 cut(s) 171, 296, 314, 329, 392, 632, 843
HpySE526I ACGT 3 cut(s) 63, 372, 378
Hsp92II CATG 3 cut(s) 136, 275, 661
Ksp22I TGATCA 2 cut(s) 31, 103
Kzo9I GATC 2 cut(s) 31, 103
LmnI GCTCC 1 cut(s) 808
Lsp1109I GCAGC 1 cut(s) 486
LweI GCATC 4 cut(s) 159, 461, 499, 735
MaeI CTAG 1 cut(s) 792
MaeII ACGT 3 cut(s) 63, 372, 378
MaeIII GTNAC 1 cut(s) 829
MalI GATC 2 cut(s) 33, 105
MboI GATC 2 cut(s) 31, 103
MboII GAAGA 2 cut(s) 137, 288
MfeI CAATTG 1 cut(s) 540
MhlI GDGCHC 1 cut(s) 322
MluCI AATT 6 cut(s) 5, 128, 143, 196, 238, 540
MlyI GAGTC 3 cut(s) 174, 436, 529
MmeI TCCRAC 1 cut(s) 714
MnlI CCTC 4 cut(s) 19, 148, 244, 612
Mph1103I ATGCAT 1 cut(s) 568
MroXI GAANNNNTTC 1 cut(s) 747
MseI TTAA 2 cut(s) 582, 597
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 1 cut(s) 838
MunI CAATTG 1 cut(s) 540
MvaI CCWGG 1 cut(s) 838
MwoI GCNNNNNNNGC 1 cut(s) 334
NdeI CATATG 1 cut(s) 568
NdeII GATC 2 cut(s) 31, 103
NlaIII CATG 3 cut(s) 136, 275, 661
NmuCI GTSAC 1 cut(s) 829
NsiI ATGCAT 1 cut(s) 568
PdmI GAANNNNTTC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 178, 292, 448
PflFI GACNNNGTC 1 cut(s) 308
PkrI GCNGC 1 cut(s) 476
PleI GAGTC 3 cut(s) 173, 436, 529
PmaCI CACGTG 1 cut(s) 379
PmlI CACGTG 1 cut(s) 379
PpsI GAGTC 3 cut(s) 173, 436, 529
Ppu21I YACGTR 1 cut(s) 379
PsiI TTATAA 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 836
PspCI CACGTG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 836
PstNI CAGNNNCTG 1 cut(s) 833
PsyI GACNNNGTC 1 cut(s) 308
RsaI GTAC 2 cut(s) 186, 191
RsaNI GTAC 2 cut(s) 185, 190
SaqAI TTAA 2 cut(s) 582, 597
SatI GCNGC 1 cut(s) 475
Sau3AI GATC 2 cut(s) 31, 103
ScaI AGTACT 1 cut(s) 186
SchI GAGTC 3 cut(s) 174, 436, 529
ScrFI CCNGG 1 cut(s) 838
SduI GDGCHC 1 cut(s) 322
SetI ASST 9 cut(s) 66, 217, 304, 375, 381, 402, 437, 528, 813
SfaNI GCATC 4 cut(s) 159, 461, 499, 735
SmlI CTYRAG 2 cut(s) 85, 221
SmoI CTYRAG 2 cut(s) 85, 221
Sse9I AATT 6 cut(s) 5, 128, 143, 196, 238, 540
SsiI CCGC 1 cut(s) 365
SspI AATATT 1 cut(s) 121
SspMI CTAG 1 cut(s) 792
StyD4I CCNGG 1 cut(s) 836
TaaI ACNGT 2 cut(s) 189, 673
TaiI ACGT 3 cut(s) 66, 375, 381
TaqI TCGA 2 cut(s) 153, 768
TasI AATT 6 cut(s) 5, 128, 143, 196, 238, 540
TatI WGTACW 2 cut(s) 184, 189
TfiI GAWTC 3 cut(s) 178, 292, 448
Tru1I TTAA 2 cut(s) 582, 597
Tru9I TTAA 2 cut(s) 582, 597
TscAI CASTG 1 cut(s) 834
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 1 cut(s) 474
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 5 cut(s) 53, 121, 260, 480, 585
TspRI CASTG 1 cut(s) 834
Tth111I GACNNNGTC 1 cut(s) 308
XapI RAATTY 2 cut(s) 5, 128
XmnI GAANNNNTTC 1 cut(s) 747
XspI CTAG 1 cut(s) 792
ZrmI AGTACT 1 cut(s) 186
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.