Prupe.6G072700_v2.0.a1

Haloacid dehalogenase-like hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0019565
Physical Location & Seq
Forward (+)
4959874 .. 4962813
2940 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G072700.1

Sequence Viewer

Length: 849 bp
ATGGATTTCCAAAACCAGTATCAGCAGGCTGATCAGATGCCAAAATATGAGTGTCTTCTCTTTGATCTAGATGATACCCTTTATCCCTTGAGTTCTGGAATGTCTAAACAATGCACCAAGAATATTGAAGAATACATGGTTCAAAAACTAGGCATAGCGGAAACAACAGTCACTCAGCTTAATCAAGTGTTATACAAGAATTATGGGACATCAATGGCTGGTCTAAGGGCCATTGGTTATAACTTTGACAATGATGACTACCATAGTTTTGTTCATGGAAGATTGCCCTATGAGGTACTAAGACCTGACCATGTCCTTAGGACTCTGCTGCTGAGCCTGCCGTATCGAAAACTCATTTTCTCAAATGGGGACAAGTTCCATGTGGCAAAAACTCTTAGCAAGCTTGGATTGAAAGACTGTTTTGAAGGAGTTATATGTTTTGAGACTCTGAATCCCATCAGTGATAATGAAGACTCAAAGTCCACAGGCTGCAGAAACGTTTTTGACCATTCTTGTTTATTTGATGCTGGATCAGCACTTCCAGTGACTCCAGTTGTTTGCAAACCATTTGCAAATGCATATGAACAAGCCTTTCAGAAAGCCAATATTAACCCTCAAACAACTCTCTTCTTTGATGATAGCATCCGCAACATACAGGCTGGAAAGGATATGGGTCTTCATACTGTATTGGTGGGCACTTCCAACAGAACAAAGGGTGTGGATTATGCAATAGAGAGCATCCACAATATCAGAGAAGCACTGCCAGAGCTTTGGGAAATCAATAATAAGAAAGGCATAAGCAGAAGCTTTTCTGGTAAGCTGGCAATGGAGACATCAGTGACTGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 Hydrolase_like

Protein Analysis

283

Amino Acids

31.74

Weight (kDa)

5.81

Isoelectric Point (pI)

42.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019565)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59490 AT5G59490
malus_domestica MD03G1088500.v1.1 MD11G1097500.v1.1
prunus_persica Prupe.6G072700_v2.0.a1
pyrus_communis pycom03g07010 pycom11g08220

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 240
AciI CCGC 2 cut(s) 158, 646
AclI AACGTT 1 cut(s) 498
AclWI GGATC 1 cut(s) 538
AfaI GTAC 1 cut(s) 297
AgsI TTSAA 4 cut(s) 128, 143, 412, 425
AhdI GACNNNNNGTC 1 cut(s) 478
AluBI AGCT 5 cut(s) 178, 403, 769, 807, 820
AluI AGCT 5 cut(s) 178, 403, 769, 807, 820
Alw26I GTCTC 2 cut(s) 437, 824
AlwI GGATC 1 cut(s) 538
AlwNI CAGNNNCTG 1 cut(s) 842
AoxI GGCC 1 cut(s) 228
ApeKI GCWGC 2 cut(s) 328, 489
Asp700I GAANNNNTTC 1 cut(s) 808
AspS9I GGNCC 1 cut(s) 228
AxyI CCTNAGG 1 cut(s) 317
BaeGI GKGCMC 1 cut(s) 698
BbsI GAAGAC 3 cut(s) 47, 477, 668
BbvI GCAGC 2 cut(s) 315, 476
BccI CCATC 1 cut(s) 464
BceAI ACGGC 1 cut(s) 325
BclI TGATCA 1 cut(s) 31
BcoDI GTCTC 2 cut(s) 437, 824
BfaI CTAG 2 cut(s) 68, 149
BfmI CTRYAG 1 cut(s) 490
BisI GCNGC 2 cut(s) 329, 490
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 2 cut(s) 330, 491
BmeRI GACNNNNNGTC 1 cut(s) 478
BmgT120I GGNCC 1 cut(s) 228
BmsI GCATC 4 cut(s) 27, 514, 651, 747
BpiI GAAGAC 3 cut(s) 47, 477, 668
BpmI CTGGAG 1 cut(s) 534
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 109
Bse1I ACTGG 3 cut(s) 16, 542, 551
Bse21I CCTNAGG 1 cut(s) 317
Bse3DI GCAATG 1 cut(s) 831
BseGI GGATG 2 cut(s) 642, 738
BseMI GCAATG 1 cut(s) 831
BseMII CTCAG 2 cut(s) 188, 323
BseNI ACTGG 3 cut(s) 16, 542, 551
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 2 cut(s) 315, 476
BshFI GGCC 1 cut(s) 230
BslFI GGGAC 2 cut(s) 220, 383
BsmAI GTCTC 2 cut(s) 437, 824
BsmFI GGGAC 2 cut(s) 220, 383
BsnI GGCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 698
Bsp143I GATC 3 cut(s) 31, 64, 530
Bsp1720I GCTNAGC 1 cut(s) 332
BspACI CCGC 2 cut(s) 158, 646
BspANI GGCC 1 cut(s) 230
BspCNI CTCAG 2 cut(s) 187, 324
BspMAI CTGCAG 1 cut(s) 494
BspPI GGATC 1 cut(s) 538
BsrDI GCAATG 1 cut(s) 831
BsrI ACTGG 3 cut(s) 16, 542, 551
BssMI GATC 3 cut(s) 31, 64, 530
Bst4CI ACNGT 3 cut(s) 169, 419, 685
Bst6I CTCTTC 1 cut(s) 632
BstC8I GCNNGC 4 cut(s) 27, 338, 401, 822
BstDEI CTNAG 6 cut(s) 174, 224, 299, 317, 332, 395
BstF5I GGATG 2 cut(s) 642, 738
BstKTI GATC 3 cut(s) 34, 67, 533
BstMAI GTCTC 2 cut(s) 437, 824
BstMBI GATC 3 cut(s) 31, 64, 530
BstMWI GCNNNNNNNGC 2 cut(s) 337, 533
BstSFI CTRYAG 1 cut(s) 490
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 2 cut(s) 315, 476
BstV2I GAAGAC 3 cut(s) 47, 477, 668
BstXI CCANNNNNNTGG 1 cut(s) 771
Bsu36I CCTNAGG 1 cut(s) 317
BsuRI GGCC 1 cut(s) 230
BtsCI GGATG 2 cut(s) 642, 738
BtsI GCAGTG 1 cut(s) 758
BtsIMutI CAGTG 4 cut(s) 466, 549, 758, 843
Cac8I GCNNGC 4 cut(s) 27, 338, 401, 822
CaiI CAGNNNCTG 1 cut(s) 842
Cfr13I GGNCC 1 cut(s) 228
Csp6I GTAC 1 cut(s) 296
CspCI CAANNNNNGTGG 2 cut(s) 699, 734
CviAII CATG 4 cut(s) 136, 275, 311, 380
CviQI GTAC 1 cut(s) 296
DdeI CTNAG 6 cut(s) 174, 224, 299, 317, 332, 395
DpnI GATC 3 cut(s) 33, 66, 532
DpnII GATC 3 cut(s) 31, 64, 530
DriI GACNNNNNGTC 1 cut(s) 478
Eam1104I CTCTTC 1 cut(s) 632
Eam1105I GACNNNNNGTC 1 cut(s) 478
EarI CTCTTC 1 cut(s) 632
Eco81I CCTNAGG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 580
FaeI CATG 4 cut(s) 139, 278, 314, 383
FaqI GGGAC 2 cut(s) 220, 383
FatI CATG 4 cut(s) 135, 274, 310, 379
FauNDI CATATG 1 cut(s) 580
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 2 cut(s) 329, 490
FokI GGATG 2 cut(s) 629, 725
Fsp4HI GCNGC 2 cut(s) 329, 490
FspBI CTAG 2 cut(s) 68, 149
GluI GCNGC 2 cut(s) 329, 490
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 1 cut(s) 230
Hin1II CATG 4 cut(s) 139, 278, 314, 383
HindIII AAGCTT 2 cut(s) 401, 805
HinfI GANTC 5 cut(s) 322, 445, 451, 473, 547
Hpy166II GTNNAC 1 cut(s) 483
Hpy188I TCNGA 4 cut(s) 36, 450, 597, 752
Hpy188III TCNNGA 2 cut(s) 68, 96
Hpy8I GTNNAC 1 cut(s) 483
HpyAV CCTTC 1 cut(s) 419
HpyCH4III ACNGT 3 cut(s) 169, 419, 685
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 6 cut(s) 114, 492, 561, 572, 578, 728
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 533
HpyF3I CTNAG 6 cut(s) 174, 224, 299, 317, 332, 395
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 4 cut(s) 139, 278, 314, 383
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 3 cut(s) 31, 64, 530
Lsp1109I GCAGC 2 cut(s) 315, 476
LweI GCATC 4 cut(s) 27, 514, 651, 747
MaeI CTAG 2 cut(s) 68, 149
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 3 cut(s) 169, 544, 838
MalI GATC 3 cut(s) 33, 66, 532
MboI GATC 3 cut(s) 31, 64, 530
MboII GAAGA 6 cut(s) 47, 140, 291, 482, 619, 668
MhlI GDGCHC 1 cut(s) 698
MluCI AATT 1 cut(s) 199
MlyI GAGTC 4 cut(s) 316, 439, 467, 541
MmeI TCCRAC 1 cut(s) 726
MnlI CCTC 2 cut(s) 286, 624
Mph1103I ATGCAT 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 808
MseI TTAA 2 cut(s) 180, 609
MwoI GCNNNNNNNGC 2 cut(s) 337, 533
NdeI CATATG 1 cut(s) 580
NdeII GATC 3 cut(s) 31, 64, 530
NlaIII CATG 4 cut(s) 139, 278, 314, 383
NmuCI GTSAC 3 cut(s) 169, 544, 838
NsiI ATGCAT 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 1 cut(s) 451
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 2 cut(s) 330, 491
PleI GAGTC 4 cut(s) 316, 439, 467, 541
PpsI GAGTC 4 cut(s) 316, 439, 467, 541
PsiI TTATAA 1 cut(s) 240
Psp1406I AACGTT 1 cut(s) 498
PspPI GGNCC 1 cut(s) 228
PstI CTGCAG 1 cut(s) 494
PstNI CAGNNNCTG 1 cut(s) 842
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 1 cut(s) 297
RsaNI GTAC 1 cut(s) 296
SaqAI TTAA 2 cut(s) 180, 609
SatI GCNGC 2 cut(s) 329, 490
Sau3AI GATC 3 cut(s) 31, 64, 530
Sau96I GGNCC 1 cut(s) 228
SchI GAGTC 4 cut(s) 316, 439, 467, 541
SduI GDGCHC 1 cut(s) 698
SetI ASST 8 cut(s) 180, 297, 307, 405, 501, 771, 809, 822
SfaNI GCATC 4 cut(s) 27, 514, 651, 747
SfcI CTRYAG 1 cut(s) 490
SmlI CTYRAG 1 cut(s) 88
SmoI CTYRAG 1 cut(s) 88
Sse9I AATT 1 cut(s) 199
SsiI CCGC 2 cut(s) 158, 646
SspI AATATT 2 cut(s) 124, 607
SspMI CTAG 2 cut(s) 68, 149
TaaI ACNGT 3 cut(s) 169, 419, 685
TaiI ACGT 1 cut(s) 501
TaqI TCGA 1 cut(s) 346
TasI AATT 1 cut(s) 199
TfiI GAWTC 1 cut(s) 451
Tru1I TTAA 2 cut(s) 180, 609
Tru9I TTAA 2 cut(s) 180, 609
TscAI CASTG 4 cut(s) 466, 549, 765, 843
TseFI GTSAC 3 cut(s) 169, 544, 838
TseI GCWGC 2 cut(s) 328, 489
Tsp45I GTSAC 3 cut(s) 169, 544, 838
TspDTI ATGAA 4 cut(s) 263, 483, 597, 668
TspRI CASTG 4 cut(s) 466, 549, 765, 843
Tth111I GACNNNGTC 1 cut(s) 311
XbaI TCTAGA 1 cut(s) 67
XmnI GAANNNNTTC 1 cut(s) 808
XspI CTAG 2 cut(s) 68, 149
Zsp2I ATGCAT 1 cut(s) 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.