AT5G59500

Phospholipid methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014320
Physical Location & Seq
Forward (+)
23985512 .. 23987382
1871 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59500.2

Sequence Viewer

Length: 1191 bp
ATGGAAGCTGTGCTTCTCTGCTCGAAGCTTGTTCCTAGAGCGGCGTCGTTGGAAGGGAACCAAAAGTTTTCGTTCAGGCATTTGAACAAACACCTTAAGTGCAATTCCCTTCGTGCTGATTCCAGAGACACTCCGTTGCTCCGAGGTTTTGAAGCGATAAAGAGTCCGTCTATCTGGAGTTGTGCGCCGTTCCCAGTGAGAAAAGGTTCATGGGTTCCACCCAGATGCTCGATTTCTAGCTCGACTGTTTCCGATTCCGATAACCCTTTCCTTAGCCAATTTCGAACCTTCTCTTTTGGGTCAATGGTGAAGAAAGTTCGTGAGTTGAAAGTGAAGCCCATGGATGTTGTGAAGTTAACTCTTTTGCTATCGATTTTGACTGTGGCTGCAAAAAAGACTTTGACTTTGGTCTTGGACCCATTCTTCTGGATGTATTTTAGCTGGACATGGTTGTTCTGGCCTTGGTTTATCGCTGTGGGTCTTGCGGGTTATGGAATTTACTGTTTCCGTAAGCATTGGCTTGGCGAAGCCAATGCCTTTGAGCAGCTTGGTATTGTAACTTCAGTCTTCACATGGCTAACACTTGTGCCTCCTGCTTATTTCAATGGTTATCTTGAAGGCTGGCCTTATGTGTTCTTCTTGGCGTATCATTACTTCTTTTTCTTCAATGTTAGCGTGAGAAAACGCCTCTATGGAGATTTCTATGCTCGCACTCATGATCCCAAATGGGATGTGAACACGCCTCTGTGGTCTCGGATTTTGTTTGGTGTTGGCATCATGGTTGGACATTGGCTTGCAGCTTTTGAAGGGCCTGAGCTTCACCGATTACCAGGGGGTTGGGCCAATGTAGGGATATGGATTTTGATTGTGATTACAATGCTTATGCATTATGATTCAACGCTATATCTTGCTAGATATTCTGAAAAGGTTGTTGTTCCAACGGCTGTTGTGCAATTTGGTCCTTATAGATGGGTCAGGCATCCGATATATGCTTCTACGATGCTTCTTTTCGCTGCATACTGCACTGCTCTGCGTGCGCCTTTGAGTCTGTTGTTTCTTTTAGCGGTTTGTTTGGTTTACTACAACAAGAAGGCAAAGATGGAAGAAGAATTGATGGTGGAGAGTTTCGGACAAAGCTATTCGGATTATGCTGATAAGGTTAGGCACAAGTTCATTCCTTTTGTTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEMT

Protein Analysis

396

Amino Acids

45.79

Weight (kDa)

9.47

Isoelectric Point (pI)

38.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEMT PF04191 183 - 277 1.4e-07 Phospholipid methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59500 AT5G59500
fragaria_vesca FvH4_3g36800 FvH4_3g36800
malus_domestica MD03G1088200.v1.1
prunus_persica Prupe.6G072500_v2.0.a1
pyrus_communis pycom03g06970
rosa_chinensis RchiOBHm_Chr5g0065861
rosa_laevigata RLG00000035822
rosa_multiflora Rmu_co8472781.1_g000001
rosa_roxburghii Rroxscaffold_1G00015300
rosa_rugosa Rorug05G0374600
rosa_samantha Rh5AG433100 Rh5BG449300 Rh5CG471600 Rh5DG462900
rosa_wichuraiana Rw5G040610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 41
AciI CCGC 3 cut(s) 41, 485, 1064
AclWI GGATC 1 cut(s) 713
AcsI RAATTY 1 cut(s) 495
AcuI CTGAAG 1 cut(s) 546
AcyI GRCGYC 1 cut(s) 44
AfiI CCNNNNNNNGG 1 cut(s) 849
AflII CTTAAG 1 cut(s) 95
AgsI TTSAA 8 cut(s) 85, 152, 328, 604, 617, 667, 806, 897
AjnI CCWGG 1 cut(s) 829
AluBI AGCT 8 cut(s) 8, 28, 240, 441, 547, 800, 817, 1137
AluI AGCT 8 cut(s) 8, 28, 240, 441, 547, 800, 817, 1137
Alw26I GTCTC 2 cut(s) 120, 756
AlwI GGATC 1 cut(s) 713
AoxI GGCC 4 cut(s) 458, 623, 809, 840
ApeKI GCWGC 4 cut(s) 386, 544, 797, 1013
ApoI RAATTY 1 cut(s) 495
ArsI GACNNNNNNTTYG 2 cut(s) 388, 420
Asp700I GAANNNNTTC 1 cut(s) 205
AspLEI GCGC 2 cut(s) 187, 1039
AspS9I GGNCC 4 cut(s) 415, 809, 840, 959
AsuHPI GGTGA 2 cut(s) 319, 812
AsuII TTCGAA 1 cut(s) 283
AvaII GGWCC 2 cut(s) 415, 959
BarI GAAGNNNNNNTAC 4 cut(s) 544, 576, 638, 670
BbsI GAAGAC 1 cut(s) 559
BbvI GCAGC 4 cut(s) 373, 556, 809, 1000
BccI CCATC 3 cut(s) 963, 1093, 1108
BceAI ACGGC 2 cut(s) 172, 957
BcgI CGANNNNNNTGC 2 cut(s) 515, 549
BciT130I CCWGG 1 cut(s) 831
BcoDI GTCTC 2 cut(s) 120, 756
BfaI CTAG 4 cut(s) 36, 237, 912, 1189
BfrI CTTAAG 1 cut(s) 95
BisI GCNGC 5 cut(s) 42, 387, 545, 798, 1014
BlsI GCNGC 5 cut(s) 43, 388, 546, 799, 1015
Bme1390I CCNGG 1 cut(s) 831
Bme18I GGWCC 2 cut(s) 415, 959
BmgT120I GGNCC 4 cut(s) 415, 809, 840, 959
BmiI GGNNCC 3 cut(s) 59, 216, 417
BmrFI CCNGG 1 cut(s) 831
BmrI ACTGGG 1 cut(s) 188
BmsI GCATC 4 cut(s) 215, 783, 988, 990
BmuI ACTGGG 1 cut(s) 188
BoxI GACNNNNGTC 1 cut(s) 407
BpiI GAAGAC 1 cut(s) 559
BpmI CTGGAG 1 cut(s) 196
Bpu10I CCTNAGC 2 cut(s) 272, 813
Bpu14I TTCGAA 1 cut(s) 283
Bsa29I ATCGAT 1 cut(s) 371
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 756
BsaJI CCNNGG 4 cut(s) 142, 339, 461, 830
Bsc4I CCNNNNNNNGG 1 cut(s) 849
Bse1I ACTGG 1 cut(s) 194
BseBI CCWGG 1 cut(s) 831
BseCI ATCGAT 1 cut(s) 371
BseDI CCNNGG 4 cut(s) 142, 339, 461, 830
BseGI GGATG 4 cut(s) 349, 435, 736, 979
BseLI CCNNNNNNNGG 1 cut(s) 849
BseMII CTCAG 1 cut(s) 804
BseNI ACTGG 1 cut(s) 194
BseXI GCAGC 4 cut(s) 373, 556, 809, 1000
BsgI GTGCAG 1 cut(s) 1006
BshFI GGCC 4 cut(s) 460, 625, 811, 842
BshVI ATCGAT 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 849
BsmAI GTCTC 2 cut(s) 120, 756
BsnI GGCC 4 cut(s) 460, 625, 811, 842
Bso31I GGTCTC 1 cut(s) 756
Bsp119I TTCGAA 1 cut(s) 283
Bsp143I GATC 1 cut(s) 718
Bsp19I CCATGG 1 cut(s) 339
BspACI CCGC 3 cut(s) 41, 485, 1064
BspANI GGCC 4 cut(s) 460, 625, 811, 842
BspCNI CTCAG 1 cut(s) 805
BspDI ATCGAT 1 cut(s) 371
BspHI TCATGA 1 cut(s) 715
BspLI GGNNCC 3 cut(s) 59, 216, 417
BspPI GGATC 1 cut(s) 713
BspT104I TTCGAA 1 cut(s) 283
BspTI CTTAAG 1 cut(s) 95
BspTNI GGTCTC 1 cut(s) 756
BsrBI CCGCTC 1 cut(s) 41
BsrI ACTGG 1 cut(s) 194
BssECI CCNNGG 4 cut(s) 142, 339, 461, 830
BssMI GATC 1 cut(s) 718
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 2 cut(s) 339, 461
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 3 cut(s) 247, 382, 503
BstACI GRCGYC 1 cut(s) 44
BstAFI CTTAAG 1 cut(s) 95
BstBI TTCGAA 1 cut(s) 283
BstC8I GCNNGC 4 cut(s) 623, 709, 795, 1035
BstDEI CTNAG 2 cut(s) 272, 813
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 4 cut(s) 349, 435, 736, 979
BstHHI GCGC 2 cut(s) 187, 1039
BstKTI GATC 1 cut(s) 721
BstMAI GTCTC 2 cut(s) 120, 756
BstMBI GATC 1 cut(s) 718
BstMWI GCNNNNNNNGC 1 cut(s) 1034
BstNI CCWGG 1 cut(s) 831
BstPAI GACNNNNGTC 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 829
BstV1I GCAGC 4 cut(s) 373, 556, 809, 1000
BstV2I GAAGAC 1 cut(s) 559
BstXI CCANNNNNNTGG 2 cut(s) 426, 837
Bsu15I ATCGAT 1 cut(s) 371
BsuRI GGCC 4 cut(s) 460, 625, 811, 842
BsuTUI ATCGAT 1 cut(s) 371
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 4 cut(s) 349, 435, 736, 979
BtsI GCAGTG 1 cut(s) 1023
BtsIMutI CAGTG 2 cut(s) 201, 1023
Cac8I GCNNGC 4 cut(s) 623, 709, 795, 1035
CciI TCATGA 1 cut(s) 715
CfoI GCGC 2 cut(s) 187, 1039
Cfr13I GGNCC 4 cut(s) 415, 809, 840, 959
ClaI ATCGAT 1 cut(s) 371
CseI GACGC 1 cut(s) 33
CviAII CATG 6 cut(s) 210, 340, 447, 573, 716, 778
DdeI CTNAG 2 cut(s) 272, 813
DpnI GATC 1 cut(s) 720
DpnII GATC 1 cut(s) 718
Eco130I CCWWGG 2 cut(s) 339, 461
Eco31I GGTCTC 1 cut(s) 756
Eco47I GGWCC 2 cut(s) 415, 959
Eco57I CTGAAG 1 cut(s) 546
EcoO109I RGGNCCY 1 cut(s) 809
EcoRII CCWGG 1 cut(s) 829
EcoT14I CCWWGG 2 cut(s) 339, 461
EcoT22I ATGCAT 1 cut(s) 888
ErhI CCWWGG 2 cut(s) 339, 461
FaeI CATG 6 cut(s) 213, 343, 450, 576, 719, 781
FalI AAGNNNNNCTT 1 cut(s) 29
FatI CATG 6 cut(s) 209, 339, 446, 572, 715, 777
FauI CCCGC 1 cut(s) 478
Fnu4HI GCNGC 5 cut(s) 42, 387, 545, 798, 1014
FokI GGATG 4 cut(s) 356, 442, 743, 966
Fsp4HI GCNGC 5 cut(s) 42, 387, 545, 798, 1014
FspBI CTAG 4 cut(s) 36, 237, 912, 1189
GlaI GCGC 2 cut(s) 186, 1038
GluI GCNGC 5 cut(s) 42, 387, 545, 798, 1014
GsuI CTGGAG 1 cut(s) 196
HaeIII GGCC 4 cut(s) 460, 625, 811, 842
HgaI GACGC 1 cut(s) 33
HhaI GCGC 2 cut(s) 187, 1039
Hin1I GRCGYC 1 cut(s) 44
Hin1II CATG 6 cut(s) 213, 343, 450, 576, 719, 781
Hin6I GCGC 2 cut(s) 185, 1037
HinP1I GCGC 2 cut(s) 185, 1037
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 5 cut(s) 119, 163, 254, 893, 1045
HpaI GTTAAC 1 cut(s) 357
HphI GGTGA 2 cut(s) 319, 812
Hpy166II GTNNAC 4 cut(s) 357, 736, 1078, 1186
Hpy188I TCNGA 8 cut(s) 143, 253, 259, 756, 922, 984, 1130, 1144
Hpy188III TCNNGA 6 cut(s) 123, 175, 320, 427, 614, 716
Hpy8I GTNNAC 4 cut(s) 357, 736, 1078, 1186
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 6 cut(s) 47, 119, 298, 611, 800, 1084
HpyCH4III ACNGT 3 cut(s) 247, 382, 503
HpyCH4V TGCA 7 cut(s) 102, 389, 797, 886, 952, 1016, 1023
HpyF10VI GCNNNNNNNGC 1 cut(s) 1034
HpyF3I CTNAG 2 cut(s) 272, 813
Hsp92I GRCGYC 1 cut(s) 44
Hsp92II CATG 6 cut(s) 213, 343, 450, 576, 719, 781
HspAI GCGC 2 cut(s) 185, 1037
KspAI GTTAAC 1 cut(s) 357
Kzo9I GATC 1 cut(s) 718
LmnI GCTCC 1 cut(s) 144
Lsp1109I GCAGC 4 cut(s) 373, 556, 809, 1000
LweI GCATC 4 cut(s) 215, 783, 988, 990
MaeI CTAG 4 cut(s) 36, 237, 912, 1189
MaeIII GTNAC 1 cut(s) 556
MalI GATC 1 cut(s) 720
MbiI CCGCTC 1 cut(s) 41
MboI GATC 1 cut(s) 718
MboII GAAGA 7 cut(s) 322, 415, 559, 628, 655, 1115, 1118
MluCI AATT 5 cut(s) 103, 278, 495, 953, 1109
MlyI GAGTC 2 cut(s) 172, 1054
MmeI TCCRAC 3 cut(s) 30, 763, 962
MnlI CCTC 4 cut(s) 137, 600, 698, 753
Mph1103I ATGCAT 1 cut(s) 888
MroXI GAANNNNTTC 1 cut(s) 205
MseI TTAA 2 cut(s) 96, 356
MslI CAYNNNNRTG 1 cut(s) 223
MspCI CTTAAG 1 cut(s) 95
MspR9I CCNGG 1 cut(s) 831
MvaI CCWGG 1 cut(s) 831
MwoI GCNNNNNNNGC 1 cut(s) 1034
NcoI CCATGG 1 cut(s) 339
NdeII GATC 1 cut(s) 718
NlaIII CATG 6 cut(s) 213, 343, 450, 576, 719, 781
NlaIV GGNNCC 3 cut(s) 59, 216, 417
NsiI ATGCAT 1 cut(s) 888
NspV TTCGAA 1 cut(s) 283
PagI TCATGA 1 cut(s) 715
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 3 cut(s) 119, 254, 893
PkrI GCNGC 5 cut(s) 43, 388, 546, 799, 1015
PleI GAGTC 2 cut(s) 171, 1053
PpsI GAGTC 2 cut(s) 171, 1053
PshAI GACNNNNGTC 1 cut(s) 407
Psp6I CCWGG 1 cut(s) 829
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 3 cut(s) 59, 216, 417
PspPI GGNCC 4 cut(s) 415, 809, 840, 959
RseI CAYNNNNRTG 1 cut(s) 223
SaqAI TTAA 2 cut(s) 96, 356
SatI GCNGC 5 cut(s) 42, 387, 545, 798, 1014
Sau3AI GATC 1 cut(s) 718
Sau96I GGNCC 4 cut(s) 415, 809, 840, 959
SchI GAGTC 2 cut(s) 172, 1054
ScrFI CCNGG 1 cut(s) 831
SfaNI GCATC 4 cut(s) 215, 783, 988, 990
SfuI TTCGAA 1 cut(s) 283
SinI GGWCC 2 cut(s) 415, 959
SmiMI CAYNNNNRTG 1 cut(s) 223
SmlI CTYRAG 1 cut(s) 95
SmoI CTYRAG 1 cut(s) 95
Sse9I AATT 5 cut(s) 103, 278, 495, 953, 1109
SsiI CCGC 3 cut(s) 41, 485, 1064
SspMI CTAG 4 cut(s) 36, 237, 912, 1189
StyD4I CCNGG 1 cut(s) 829
StyI CCWWGG 2 cut(s) 339, 461
TaaI ACNGT 3 cut(s) 247, 382, 503
TaqI TCGA 5 cut(s) 23, 230, 242, 283, 371
TasI AATT 5 cut(s) 103, 278, 495, 953, 1109
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 3 cut(s) 119, 254, 893
Tru1I TTAA 2 cut(s) 96, 356
Tru9I TTAA 2 cut(s) 96, 356
TscAI CASTG 2 cut(s) 201, 1030
TseI GCWGC 4 cut(s) 386, 544, 797, 1013
TspDTI ATGAA 2 cut(s) 198, 1162
TspGWI ACGGA 3 cut(s) 123, 156, 497
TspRI CASTG 2 cut(s) 201, 1030
Vha464I CTTAAG 1 cut(s) 95
VpaK11BI GGWCC 2 cut(s) 415, 959
XapI RAATTY 1 cut(s) 495
XmnI GAANNNNTTC 1 cut(s) 205
XspI CTAG 4 cut(s) 36, 237, 912, 1189
Zsp2I ATGCAT 1 cut(s) 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.