MD03G1088200.v1.1

Isoprenylcysteine carboxyl methyltransferase (ICMT) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0014320
Physical Location & Seq
Reverse (-)
7292482 .. 7293696
1215 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1088200.v1.1.491

Sequence Viewer

Length: 1215 bp
ATGGAAGCCAAGTCAATGTTGCTTCACTCCAAAACAATCCCTTTCTGTTCCACCTTCACAGACAGACCCAATTTCTCTTTCAGGCATTTCAGCAAACACCTTCCGTGCAATCCAATAAACCACAGCTCTACCTTCCGCCATAGCCTTAAACCCCTCAAAACCCCAGCAAGTTTATGTCTTTCACAAAACCCATCATTCTTATCATCCCCAACAAGAACAAGAAGAAGCACCCATTTGTTAGCTCCACTCAAATGCTCATTTTCCAGCACCTCAAGCCACCCAGAATCTCAAACTCCATTGCTGAAGCTTTTCAATAACCTCTCATTTGATTCGTTGAAAACTACCCTTTTGAAATTAACCCCATTTGATGTGATTAAGTGGTCCGGGATTTTGTCAATTGCAATTGCAGCCACAAGGGGGACTCTGAATTTGCTATTGAGCCCTTTTTTCTGGATGTACTTCAGCTGGACTTGGATGTTTTGGCCTTGGTTTCTGGCTGTATCAGTTGCGGTTTACGGGTTATACTGCTTTAGGAAGCATTTGCGCTGTGAAGCAAATGTGTTTGAGCAGCTTGCGATTGTTACCTCAGTGTTTTCTTGGCTCACACTTGTGCCACCTGCCCATTTCAATGGCTTCCTTGAAGGTTGGCCTTATGTATTCTTCTTTGTGTACCATTATTTCTTTTTCTTCAATGTTAGTGTGAGGAAACGGTTATATGGGGATTACTATGCCCGCCCACATGATCCCAAGTGGGATGTGAGCCCACCGAAATGGTTGCGCCTTTTGTTCTGTGTTGGGGTCATGGCTGGTCACTGGCTGGCTGCTTTTGAAGGACCCGAGCTTCATCGCATTCCTGGCAAGTGGAGCAATGTTCCTATTTGGATTTTGATATTGGTGACTCTGCTAATGCAGTATAACTCAACATTGTATCTTGCAAAGTATTCAGAAAAGGTTGTGGTGCCAACTGCTGTTGTGCAGTTTGGGCCTTACCGGTGGGTGCGTCATCCAATATATGCTTCTACAATGCTTCTGTTTGCCACATATTTCATTGCGCTTCGGGCACCTTTGAGCTTGCTATTCATTGTAGCGGTTTGTTCGTTGTACTATGATCAGAAGGCCAAAATGGAGGAGGCCTTGATGGTTGAGACTTTTGGGGAGAGGTATACGGATTATGCAAGTAAAGTTAGGTACAAGTTCATTCCTTTTATCTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEMT

Protein Analysis

405

Amino Acids

47.06

Weight (kDa)

9.63

Isoelectric Point (pI)

36.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEMT PF04191 322 - 387 3.3e-07 Phospholipid methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59500 AT5G59500
fragaria_vesca FvH4_3g36800 FvH4_3g36800
malus_domestica MD03G1088200.v1.1
prunus_persica Prupe.6G072500_v2.0.a1
pyrus_communis pycom03g06970
rosa_chinensis RchiOBHm_Chr5g0065861
rosa_laevigata RLG00000035822
rosa_multiflora Rmu_co8472781.1_g000001
rosa_roxburghii Rroxscaffold_1G00015300
rosa_rugosa Rorug05G0374600
rosa_samantha Rh5AG433100 Rh5BG449300 Rh5CG471600 Rh5DG462900
rosa_wichuraiana Rw5G040610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 625
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 2 cut(s) 958, 1060
AccI GTMKAC 1 cut(s) 1163
AciI CCGC 4 cut(s) 136, 509, 733, 1088
AclWI GGATC 1 cut(s) 737
AcsI RAATTY 1 cut(s) 427
AcuI CTGAAG 2 cut(s) 323, 445
AfaI GTAC 4 cut(s) 458, 671, 1103, 1190
AfiI CCNNNNNNNGG 1 cut(s) 417
AgeI ACCGGT 1 cut(s) 990
AgsI TTSAA 7 cut(s) 313, 337, 352, 628, 641, 691, 830
AjnI CCWGG 1 cut(s) 853
AjuI GAANNNNNNNTTGG 4 cut(s) 62, 94, 1000, 1032
AleI CACNNNNGTG 1 cut(s) 608
AluBI AGCT 7 cut(s) 126, 242, 307, 465, 571, 841, 1071
AluI AGCT 7 cut(s) 126, 242, 307, 465, 571, 841, 1071
Alw26I GTCTC 1 cut(s) 1139
AlwI GGATC 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 836
AoxI GGCC 5 cut(s) 482, 647, 983, 1116, 1131
ApeKI GCWGC 3 cut(s) 407, 568, 821
ApoI RAATTY 1 cut(s) 427
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AsiGI ACCGGT 1 cut(s) 990
Asp700I GAANNNNTTC 1 cut(s) 308
AspLEI GCGC 3 cut(s) 546, 780, 1054
AspS9I GGNCC 3 cut(s) 381, 833, 983
AsuC2I CCSGG 1 cut(s) 385
AsuHPI GGTGA 1 cut(s) 907
AvaI CYCGRG 1 cut(s) 836
AvaII GGWCC 2 cut(s) 381, 833
BaeGI GKGCMC 1 cut(s) 1063
BanI GGYRCC 2 cut(s) 958, 1060
BanII GRGCYC 2 cut(s) 443, 764
BbvI GCAGC 3 cut(s) 419, 580, 808
BccI CCATC 2 cut(s) 199, 1132
BcgI CGANNNNNNTGC 2 cut(s) 757, 791
BciT130I CCWGG 1 cut(s) 855
BclI TGATCA 1 cut(s) 1108
BcnI CCSGG 1 cut(s) 385
BcoDI GTCTC 1 cut(s) 1139
BfuAI ACCTGC 1 cut(s) 625
BisI GCNGC 3 cut(s) 408, 569, 822
BlsI GCNGC 3 cut(s) 409, 570, 823
Bme1390I CCNGG 2 cut(s) 385, 855
Bme18I GGWCC 2 cut(s) 381, 833
BmeT110I CYCGRG 1 cut(s) 836
BmgT120I GGNCC 3 cut(s) 381, 833, 983
BmiI GGNNCC 3 cut(s) 835, 960, 1062
BmrFI CCNGG 2 cut(s) 385, 855
BpuEI CTTGAG 1 cut(s) 256
BpuMI CCSGG 1 cut(s) 385
BsaJI CCNNGG 1 cut(s) 485
BsaWI WCCGGW 1 cut(s) 990
Bsc4I CCNNNNNNNGG 1 cut(s) 417
Bse118I RCCGGY 1 cut(s) 990
Bse1I ACTGG 1 cut(s) 818
Bse3DI GCAATG 3 cut(s) 296, 874, 1047
BseBI CCWGG 1 cut(s) 855
BseDI CCNNGG 1 cut(s) 485
BseGI GGATG 5 cut(s) 203, 459, 480, 760, 1003
BseLI CCNNNNNNNGG 1 cut(s) 417
BseMI GCAATG 3 cut(s) 296, 874, 1047
BseMII CTCAG 1 cut(s) 600
BseNI ACTGG 1 cut(s) 818
BseRI GAGGAG 1 cut(s) 1142
BseSI GKGCMC 1 cut(s) 1063
BseXI GCAGC 3 cut(s) 419, 580, 808
BseYI CCCAGC 1 cut(s) 163
BsgI GTGCAG 1 cut(s) 995
BshFI GGCC 5 cut(s) 484, 649, 985, 1118, 1133
BshNI GGYRCC 2 cut(s) 958, 1060
BshTI ACCGGT 1 cut(s) 990
BsiHKCI CYCGRG 1 cut(s) 836
BsiSI CCGG 2 cut(s) 384, 991
BslFI GGGAC 1 cut(s) 433
BslI CCNNNNNNNGG 1 cut(s) 417
BsmAI GTCTC 1 cut(s) 1139
BsmFI GGGAC 1 cut(s) 433
BsmI GAATGC 1 cut(s) 849
BsnI GGCC 5 cut(s) 484, 649, 985, 1118, 1133
BsoBI CYCGRG 1 cut(s) 836
Bsp1286I GDGCHC 3 cut(s) 443, 764, 1063
Bsp143I GATC 2 cut(s) 742, 1108
BspACI CCGC 4 cut(s) 136, 509, 733, 1088
BspANI GGCC 5 cut(s) 484, 649, 985, 1118, 1133
BspCNI CTCAG 1 cut(s) 599
BspLI GGNNCC 3 cut(s) 835, 960, 1062
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 1 cut(s) 737
BspT107I GGYRCC 2 cut(s) 958, 1060
BsrDI GCAATG 3 cut(s) 296, 874, 1047
BsrFI RCCGGY 1 cut(s) 990
BsrI ACTGG 1 cut(s) 818
BssAI RCCGGY 1 cut(s) 990
BssECI CCNNGG 1 cut(s) 485
BssMI GATC 2 cut(s) 742, 1108
BssNAI GTATAC 1 cut(s) 1164
BssT1I CCWWGG 1 cut(s) 485
Bst1107I GTATAC 1 cut(s) 1164
Bst2UI CCWGG 1 cut(s) 855
Bst4CI ACNGT 1 cut(s) 711
BstC8I GCNNGC 4 cut(s) 573, 733, 819, 1073
BstDEI CTNAG 1 cut(s) 586
BstF5I GGATG 5 cut(s) 203, 459, 480, 760, 1003
BstHHI GCGC 3 cut(s) 546, 780, 1054
BstKTI GATC 2 cut(s) 745, 1111
BstMAI GTCTC 1 cut(s) 1139
BstMBI GATC 2 cut(s) 742, 1108
BstMWI GCNNNNNNNGC 6 cut(s) 273, 407, 855, 864, 982, 1058
BstNI CCWGG 1 cut(s) 855
BstSCI CCNGG 2 cut(s) 383, 853
BstSLI GKGCMC 1 cut(s) 1063
BstV1I GCAGC 3 cut(s) 419, 580, 808
BstXI CCANNNNNNTGG 2 cut(s) 629, 771
BstZ17I GTATAC 1 cut(s) 1164
BsuRI GGCC 5 cut(s) 484, 649, 985, 1118, 1133
BtgZI GCGATG 1 cut(s) 830
BtsCI GGATG 5 cut(s) 203, 459, 480, 760, 1003
BtsIMutI CAGTG 2 cut(s) 594, 811
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 4 cut(s) 573, 733, 819, 1073
CfoI GCGC 3 cut(s) 546, 780, 1054
Cfr10I RCCGGY 1 cut(s) 990
Cfr13I GGNCC 3 cut(s) 381, 833, 983
CseI GACGC 1 cut(s) 989
Csp6I GTAC 4 cut(s) 457, 670, 1102, 1189
CspAI ACCGGT 1 cut(s) 990
CviAII CATG 2 cut(s) 740, 802
CviQI GTAC 4 cut(s) 457, 670, 1102, 1189
DdeI CTNAG 1 cut(s) 586
DpnI GATC 2 cut(s) 744, 1110
DpnII GATC 2 cut(s) 742, 1108
EciI GGCGGA 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 485
Eco147I AGGCCT 1 cut(s) 1133
Eco24I GRGCYC 2 cut(s) 443, 764
Eco47I GGWCC 2 cut(s) 381, 833
Eco57I CTGAAG 2 cut(s) 323, 445
Eco88I CYCGRG 1 cut(s) 836
EcoO109I RGGNCCY 1 cut(s) 833
EcoRII CCWGG 1 cut(s) 853
EcoT14I CCWWGG 1 cut(s) 485
EcoT38I GRGCYC 2 cut(s) 443, 764
ErhI CCWWGG 1 cut(s) 485
FaeI CATG 2 cut(s) 743, 805
FaqI GGGAC 1 cut(s) 433
FatI CATG 2 cut(s) 739, 801
FauI CCCGC 1 cut(s) 740
FbaI TGATCA 1 cut(s) 1108
FblI GTMKAC 1 cut(s) 1163
Fnu4HI GCNGC 3 cut(s) 408, 569, 822
FokI GGATG 5 cut(s) 190, 466, 487, 767, 990
FriOI GRGCYC 2 cut(s) 443, 764
Fsp4HI GCNGC 3 cut(s) 408, 569, 822
GlaI GCGC 3 cut(s) 545, 779, 1053
GluI GCNGC 3 cut(s) 408, 569, 822
GsaI CCCAGC 1 cut(s) 167
HaeIII GGCC 5 cut(s) 484, 649, 985, 1118, 1133
HapII CCGG 2 cut(s) 384, 991
HgaI GACGC 1 cut(s) 989
HhaI GCGC 3 cut(s) 546, 780, 1054
Hin1II CATG 2 cut(s) 743, 805
Hin6I GCGC 3 cut(s) 544, 778, 1052
HinP1I GCGC 3 cut(s) 544, 778, 1052
HindIII AAGCTT 1 cut(s) 305
HinfI GANTC 4 cut(s) 284, 329, 421, 898
HpaII CCGG 2 cut(s) 384, 991
HphI GGTGA 1 cut(s) 907
Hpy166II GTNNAC 3 cut(s) 514, 670, 1164
Hpy188I TCNGA 3 cut(s) 426, 946, 1113
Hpy188III TCNNGA 1 cut(s) 451
Hpy8I GTNNAC 3 cut(s) 514, 670, 1164
HpyAV CCTTC 6 cut(s) 64, 110, 142, 635, 824, 1108
HpyCH4III ACNGT 1 cut(s) 711
HpyCH4V TGCA 7 cut(s) 108, 401, 407, 910, 935, 976, 1175
HpyF10VI GCNNNNNNNGC 6 cut(s) 273, 407, 855, 864, 982, 1058
HpyF3I CTNAG 1 cut(s) 586
Hsp92II CATG 2 cut(s) 743, 805
HspAI GCGC 3 cut(s) 544, 778, 1052
Ksp22I TGATCA 1 cut(s) 1108
Kzo9I GATC 2 cut(s) 742, 1108
LmnI GCTCC 2 cut(s) 247, 864
Lsp1109I GCAGC 3 cut(s) 419, 580, 808
MaeIII GTNAC 3 cut(s) 580, 809, 895
MalI GATC 2 cut(s) 744, 1110
MboI GATC 2 cut(s) 742, 1108
MboII GAAGA 3 cut(s) 234, 652, 679
MfeI CAATTG 2 cut(s) 396, 402
MhlI GDGCHC 3 cut(s) 443, 764, 1063
MluCI AATT 5 cut(s) 70, 353, 396, 402, 427
MlyI GAGTC 2 cut(s) 415, 892
MnlI CCTC 8 cut(s) 164, 280, 329, 595, 696, 1120, 1123, 1152
MroXI GAANNNNTTC 1 cut(s) 308
MseI TTAA 3 cut(s) 147, 356, 375
MslI CAYNNNNRTG 4 cut(s) 250, 608, 627, 769
MspA1I CMGCKG 1 cut(s) 465
MspI CCGG 2 cut(s) 384, 991
MspR9I CCNGG 2 cut(s) 385, 855
MunI CAATTG 2 cut(s) 396, 402
Mva1269I GAATGC 1 cut(s) 849
MvaI CCWGG 1 cut(s) 855
MwoI GCNNNNNNNGC 6 cut(s) 273, 407, 855, 864, 982, 1058
NciI CCSGG 1 cut(s) 385
NdeII GATC 2 cut(s) 742, 1108
NlaIII CATG 2 cut(s) 743, 805
NlaIV GGNNCC 3 cut(s) 835, 960, 1062
NmuCI GTSAC 2 cut(s) 809, 895
OliI CACNNNNGTG 1 cut(s) 608
PaqCI CACCTGC 1 cut(s) 625
PceI AGGCCT 1 cut(s) 1133
PctI GAATGC 1 cut(s) 849
PdmI GAANNNNTTC 1 cut(s) 308
PfeI GAWTC 2 cut(s) 284, 329
PfoI TCCNGGA 1 cut(s) 383
PinAI ACCGGT 1 cut(s) 990
PkrI GCNGC 3 cut(s) 409, 570, 823
PleI GAGTC 2 cut(s) 415, 892
PpsI GAGTC 2 cut(s) 415, 892
PpuMI RGGWCCY 1 cut(s) 833
Psp5II RGGWCCY 1 cut(s) 833
Psp6I CCWGG 1 cut(s) 853
PspFI CCCAGC 1 cut(s) 163
PspGI CCWGG 1 cut(s) 853
PspN4I GGNNCC 3 cut(s) 835, 960, 1062
PspPI GGNCC 3 cut(s) 381, 833, 983
PspPPI RGGWCCY 1 cut(s) 833
PvuII CAGCTG 1 cut(s) 465
RsaI GTAC 4 cut(s) 458, 671, 1103, 1190
RsaNI GTAC 4 cut(s) 457, 670, 1102, 1189
RseI CAYNNNNRTG 4 cut(s) 250, 608, 627, 769
SaqAI TTAA 3 cut(s) 147, 356, 375
SatI GCNGC 3 cut(s) 408, 569, 822
Sau3AI GATC 2 cut(s) 742, 1108
Sau96I GGNCC 3 cut(s) 381, 833, 983
SchI GAGTC 2 cut(s) 415, 892
ScrFI CCNGG 2 cut(s) 385, 855
SduI GDGCHC 3 cut(s) 443, 764, 1063
SinI GGWCC 2 cut(s) 381, 833
SmiMI CAYNNNNRTG 4 cut(s) 250, 608, 627, 769
SmlI CTYRAG 1 cut(s) 271
SmoI CTYRAG 1 cut(s) 271
Sse9I AATT 5 cut(s) 70, 353, 396, 402, 427
SseBI AGGCCT 1 cut(s) 1133
SsiI CCGC 4 cut(s) 136, 509, 733, 1088
StuI AGGCCT 1 cut(s) 1133
StyD4I CCNGG 2 cut(s) 383, 853
StyI CCWWGG 1 cut(s) 485
TaaI ACNGT 1 cut(s) 711
TasI AATT 5 cut(s) 70, 353, 396, 402, 427
TatI WGTACW 2 cut(s) 456, 1101
TfiI GAWTC 2 cut(s) 284, 329
Tru1I TTAA 3 cut(s) 147, 356, 375
Tru9I TTAA 3 cut(s) 147, 356, 375
TscAI CASTG 2 cut(s) 594, 818
TseFI GTSAC 2 cut(s) 809, 895
TseI GCWGC 3 cut(s) 407, 568, 821
Tsp45I GTSAC 2 cut(s) 809, 895
TspDTI ATGAA 4 cut(s) 833, 1036, 1069, 1186
TspGWI ACGGA 2 cut(s) 93, 1181
TspRI CASTG 2 cut(s) 594, 818
VpaK11BI GGWCC 2 cut(s) 381, 833
XapI RAATTY 1 cut(s) 427
XmiI GTMKAC 1 cut(s) 1163
XmnI GAANNNNTTC 1 cut(s) 308
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.