Rh5AG433100

Isoprenylcysteine carboxyl methyltransferase (ICMT) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0014320
Physical Location & Seq
Forward (+)
74408595 .. 74410075
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG433100.1

Sequence Viewer

Length: 1203 bp
ATGGAAGCCAAGTCACTCTTTCTCCACTCCAAAACACTCCCACTCTGTTATACTTTCACAGATAGACCCAACATCTCTCTCAGGCATTTCAGCACACACCTTCAAGTCAATTCCATAAACCCCAATTCTCACTTCCACCAGAGCCTTAAACCCCTCAAAACCCCATCAAAATTATGTCTTTCACACAACCCACTATCCTTATCATCACCAACAAGAAGAACCCAGTTGCTAAGTCCTCTCAAATGCTCCTACTCCAGCACCACAAGCCCAGAATCTCAGAACCCATTGCTAAAACCCTTCAAGAACCTCCCTTTTGATTCATTGAAAGCTACCCTTTTCAAATTAACCCCCTTTGATGTCATAAAGTGGTCTGGGATCTTATCAATTGCAATTGCAGCCACAAAATGGACCTCCAATTTGGTCTTCAGCCCCTTTTTCTGGATGTACTTCAGCTGGACTTGGATGTTCTGGCCATGGTTTCTGGCTGTTTCGGTAGGCCTTTATGGGTTATACTGTTTTCGAAAGCATTTACTTGGTGAAGCAAGCATATTTGAGCAGCTTGCTGTTGTTACCTCAGTGTTCACTTGGCTCACACTTGTCCCACCTGCCCATTTCAATGGCTTCCTTGAAGGTTGGCCTTATGTGTTTTTCCTTGTGTACCATTATTTCTTTTTCTTCAATGTTAGTGTGAGAAAACGTTTGTATGGAGATTACTACGCTCGCCCACATGACCCCAAGTGGGATGTGAGCCCTCCGAAGTGGTACCGGCTTTTGTTCAGTGTTGGGGTCATGGTTGGGCACTGGCTAGCTGCATTTGAAGGGCCAGAGCTGCACCGTATTCCTGGCGGTTGGAGCAATTTGGGTATTTGGGGCTTGATAGTTGTCACTCTGCTAATGCAGTATAACTCAACATTGTATCTTGCAAAGTATTCCGAAAAGGTGGTGGTGCCAACGGCTGTTGTGCAGTTTGGGCCATACCGGTGGGTGCGCCATCCTATATATGCATCTACAATGCTTCTGTTTGCCACTTATTGTGTTGCCCTTCGAGCACCTTTGAGCTTGCTATTCATTGTAGCAGTTTGTGTGTTGTACTATGATCAGAAGGCAAAACTGGAGGAGGCTTTGATGATTGAGACTTTTGGGGAGAGGTATATGGAGTATGCAAGTAAGGTTAGATACAAGTTCATTCCTTTTATCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PEMT

Protein Analysis

400

Amino Acids

46.44

Weight (kDa)

9.49

Isoelectric Point (pI)

36.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PEMT PF04191 318 - 383 5.8e-07 Phospholipid methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014320)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59500 AT5G59500
fragaria_vesca FvH4_3g36800 FvH4_3g36800
malus_domestica MD03G1088200.v1.1
prunus_persica Prupe.6G072500_v2.0.a1
pyrus_communis pycom03g06970
rosa_chinensis RchiOBHm_Chr5g0065861
rosa_laevigata RLG00000035822
rosa_multiflora Rmu_co8472781.1_g000001
rosa_roxburghii Rroxscaffold_1G00015300
rosa_rugosa Rorug05G0374600
rosa_samantha Rh5AG433100 Rh5BG449300 Rh5CG471600 Rh5DG462900
rosa_wichuraiana Rw5G040610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 613
Acc36I ACCTGC 1 cut(s) 613
Acc65I GGTACC 1 cut(s) 762
AccB1I GGYRCC 2 cut(s) 762, 946
AccB7I CCANNNNNTGG 1 cut(s) 405
AciI CCGC 1 cut(s) 846
AclI AACGTT 1 cut(s) 697
AclWI GGATC 1 cut(s) 383
AcoI YGGCCR 1 cut(s) 470
AcuI CTGAAG 2 cut(s) 409, 433
AfaI GTAC 4 cut(s) 446, 659, 764, 1091
AfiI CCNNNNNNNGG 3 cut(s) 405, 438, 739
AgeI ACCGGT 1 cut(s) 978
AgsI TTSAA 8 cut(s) 104, 301, 325, 340, 616, 629, 679, 818
AjnI CCWGG 1 cut(s) 841
AjuI GAANNNNNNNTTGG 4 cut(s) 116, 148, 407, 439
AluBI AGCT 6 cut(s) 329, 453, 559, 809, 829, 1059
AluI AGCT 6 cut(s) 329, 453, 559, 809, 829, 1059
Alw21I GWGCWC 1 cut(s) 1051
Alw26I GTCTC 1 cut(s) 1127
AlwI GGATC 1 cut(s) 383
AoxI GGCC 5 cut(s) 470, 496, 635, 821, 971
ApeKI GCWGC 4 cut(s) 395, 556, 809, 829
ArsI GACNNNNNNTTYG 2 cut(s) 400, 432
AsiGI ACCGGT 1 cut(s) 978
Asp718I GGTACC 1 cut(s) 762
AspLEI GCGC 1 cut(s) 990
AspS9I GGNCC 3 cut(s) 408, 821, 971
AsuHPI GGTGA 2 cut(s) 198, 548
AsuII TTCGAA 1 cut(s) 520
AsuNHI GCTAGC 1 cut(s) 805
AvaII GGWCC 1 cut(s) 408
BaeGI GKGCMC 1 cut(s) 801
BalI TGGCCA 1 cut(s) 472
BanI GGYRCC 2 cut(s) 762, 946
BanII GRGCYC 1 cut(s) 752
BbsI GAAGAC 1 cut(s) 415
Bbv12I GWGCWC 1 cut(s) 1051
BbvI GCAGC 4 cut(s) 407, 568, 796, 816
BccI CCATC 2 cut(s) 172, 999
BceAI ACGGC 1 cut(s) 969
BciT130I CCWGG 1 cut(s) 843
BclI TGATCA 1 cut(s) 1096
BcoDI GTCTC 1 cut(s) 1127
BfaI CTAG 1 cut(s) 806
BfuAI ACCTGC 1 cut(s) 613
BisI GCNGC 4 cut(s) 396, 557, 810, 830
BlsI GCNGC 4 cut(s) 397, 558, 811, 831
Bme1390I CCNGG 1 cut(s) 843
Bme18I GGWCC 1 cut(s) 408
BmgT120I GGNCC 3 cut(s) 408, 821, 971
BmiI GGNNCC 2 cut(s) 764, 948
BmrFI CCNGG 1 cut(s) 843
BmrI ACTGGG 1 cut(s) 217
BmsI GCATC 1 cut(s) 1013
BmtI GCTAGC 1 cut(s) 809
BmuI ACTGGG 1 cut(s) 217
BpiI GAAGAC 1 cut(s) 415
BpmI CTGGAG 2 cut(s) 238, 1133
Bpu14I TTCGAA 1 cut(s) 520
BsaJI CCNNGG 1 cut(s) 473
BsaWI WCCGGW 1 cut(s) 978
BsaXI ACNNNNNCTCC 2 cut(s) 6, 36
Bsc4I CCNNNNNNNGG 3 cut(s) 405, 438, 739
Bse118I RCCGGY 2 cut(s) 765, 978
Bse1I ACTGG 3 cut(s) 223, 806, 1116
Bse3DI GCAATG 1 cut(s) 284
BseBI CCWGG 1 cut(s) 843
BseDI CCNNGG 1 cut(s) 473
BseGI GGATG 4 cut(s) 447, 468, 748, 991
BseLI CCNNNNNNNGG 3 cut(s) 405, 438, 739
BseMI GCAATG 1 cut(s) 284
BseMII CTCAG 3 cut(s) 94, 290, 588
BseNI ACTGG 3 cut(s) 223, 806, 1116
BseRI GAGGAG 1 cut(s) 1130
BseSI GKGCMC 1 cut(s) 801
BseXI GCAGC 4 cut(s) 407, 568, 796, 816
BsgI GTGCAG 2 cut(s) 815, 983
BshFI GGCC 5 cut(s) 472, 498, 637, 823, 973
BshNI GGYRCC 2 cut(s) 762, 946
BshTI ACCGGT 1 cut(s) 978
BsiHKAI GWGCWC 1 cut(s) 1051
BsiSI CCGG 2 cut(s) 766, 979
BslFI GGGAC 1 cut(s) 584
BslI CCNNNNNNNGG 3 cut(s) 405, 438, 739
BsmAI GTCTC 1 cut(s) 1127
BsmFI GGGAC 1 cut(s) 584
BsnI GGCC 5 cut(s) 472, 498, 637, 823, 973
Bsp119I TTCGAA 1 cut(s) 520
Bsp1286I GDGCHC 3 cut(s) 752, 801, 1051
Bsp143I GATC 2 cut(s) 375, 1096
Bsp19I CCATGG 1 cut(s) 473
BspACI CCGC 1 cut(s) 846
BspANI GGCC 5 cut(s) 472, 498, 637, 823, 973
BspCNI CTCAG 3 cut(s) 93, 289, 587
BspLI GGNNCC 2 cut(s) 764, 948
BspMI ACCTGC 1 cut(s) 613
BspOI GCTAGC 1 cut(s) 809
BspPI GGATC 1 cut(s) 383
BspT104I TTCGAA 1 cut(s) 520
BspT107I GGYRCC 2 cut(s) 762, 946
BsrDI GCAATG 1 cut(s) 284
BsrFI RCCGGY 2 cut(s) 765, 978
BsrI ACTGG 3 cut(s) 223, 806, 1116
BssAI RCCGGY 2 cut(s) 765, 978
BssECI CCNNGG 1 cut(s) 473
BssMI GATC 2 cut(s) 375, 1096
BssT1I CCWWGG 1 cut(s) 473
Bst2UI CCWGG 1 cut(s) 843
Bst4CI ACNGT 2 cut(s) 515, 836
BstBI TTCGAA 1 cut(s) 520
BstC8I GCNNGC 5 cut(s) 544, 561, 721, 807, 1061
BstDEI CTNAG 4 cut(s) 80, 230, 276, 574
BstDSI CCRYGG 1 cut(s) 473
BstF5I GGATG 4 cut(s) 447, 468, 748, 991
BstHHI GCGC 1 cut(s) 990
BstKTI GATC 2 cut(s) 378, 1099
BstMAI GTCTC 1 cut(s) 1127
BstMBI GATC 2 cut(s) 375, 1096
BstMWI GCNNNNNNNGC 6 cut(s) 264, 395, 829, 852, 970, 1046
BstNI CCWGG 1 cut(s) 843
BstSCI CCNGG 1 cut(s) 841
BstSLI GKGCMC 1 cut(s) 801
BstV1I GCAGC 4 cut(s) 407, 568, 796, 816
BstV2I GAAGAC 1 cut(s) 415
BstX2I RGATCY 1 cut(s) 375
BstXI CCANNNNNNTGG 2 cut(s) 617, 981
BstYI RGATCY 1 cut(s) 375
BsuRI GGCC 5 cut(s) 472, 498, 637, 823, 973
BtgI CCRYGG 1 cut(s) 473
BtsCI GGATG 4 cut(s) 447, 468, 748, 991
BtsIMutI CAGTG 3 cut(s) 582, 784, 799
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 5 cut(s) 544, 561, 721, 807, 1061
CfoI GCGC 1 cut(s) 990
Cfr10I RCCGGY 2 cut(s) 765, 978
Cfr13I GGNCC 3 cut(s) 408, 821, 971
Csp6I GTAC 4 cut(s) 445, 658, 763, 1090
CspAI ACCGGT 1 cut(s) 978
CviAII CATG 3 cut(s) 474, 728, 790
CviQI GTAC 4 cut(s) 445, 658, 763, 1090
DdeI CTNAG 4 cut(s) 80, 230, 276, 574
DpnI GATC 2 cut(s) 377, 1098
DpnII GATC 2 cut(s) 375, 1096
EaeI YGGCCR 1 cut(s) 470
Eco130I CCWWGG 1 cut(s) 473
Eco147I AGGCCT 1 cut(s) 498
Eco24I GRGCYC 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 408
Eco57I CTGAAG 2 cut(s) 409, 433
EcoRII CCWGG 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 473
EcoT22I ATGCAT 1 cut(s) 1006
EcoT38I GRGCYC 1 cut(s) 752
ErhI CCWWGG 1 cut(s) 473
FaeI CATG 3 cut(s) 477, 731, 793
FalI AAGNNNNNCTT 3 cut(s) 34, 318, 350
FaqI GGGAC 1 cut(s) 584
FatI CATG 3 cut(s) 473, 727, 789
FbaI TGATCA 1 cut(s) 1096
Fnu4HI GCNGC 4 cut(s) 396, 557, 810, 830
FokI GGATG 4 cut(s) 454, 475, 755, 978
FriOI GRGCYC 1 cut(s) 752
Fsp4HI GCNGC 4 cut(s) 396, 557, 810, 830
FspBI CTAG 1 cut(s) 806
GlaI GCGC 1 cut(s) 989
GluI GCNGC 4 cut(s) 396, 557, 810, 830
GsuI CTGGAG 2 cut(s) 238, 1133
HaeIII GGCC 5 cut(s) 472, 498, 637, 823, 973
HapII CCGG 2 cut(s) 766, 979
HhaI GCGC 1 cut(s) 990
Hin1II CATG 3 cut(s) 477, 731, 793
Hin6I GCGC 1 cut(s) 988
HinP1I GCGC 1 cut(s) 988
HinfI GANTC 2 cut(s) 272, 317
HpaII CCGG 2 cut(s) 766, 979
HphI GGTGA 2 cut(s) 198, 548
Hpy166II GTNNAC 2 cut(s) 582, 658
Hpy188I TCNGA 4 cut(s) 279, 756, 934, 1101
Hpy188III TCNNGA 2 cut(s) 301, 439
Hpy8I GTNNAC 2 cut(s) 582, 658
HpyAV CCTTC 6 cut(s) 110, 307, 623, 812, 1052, 1096
HpyCH4III ACNGT 2 cut(s) 515, 836
HpyCH4IV ACGT 1 cut(s) 697
HpyCH4V TGCA 9 cut(s) 389, 395, 812, 832, 898, 923, 964, 1004, 1163
HpyF10VI GCNNNNNNNGC 6 cut(s) 264, 395, 829, 852, 970, 1046
HpyF3I CTNAG 4 cut(s) 80, 230, 276, 574
HpySE526I ACGT 1 cut(s) 697
Hsp92II CATG 3 cut(s) 477, 731, 793
HspAI GCGC 1 cut(s) 988
KpnI GGTACC 1 cut(s) 766
Ksp22I TGATCA 1 cut(s) 1096
Kzo9I GATC 2 cut(s) 375, 1096
LmnI GCTCC 2 cut(s) 251, 852
Lsp1109I GCAGC 4 cut(s) 407, 568, 796, 816
LweI GCATC 1 cut(s) 1013
MaeI CTAG 1 cut(s) 806
MaeII ACGT 1 cut(s) 697
MaeIII GTNAC 3 cut(s) 12, 568, 883
MalI GATC 2 cut(s) 377, 1098
MboI GATC 2 cut(s) 375, 1096
MboII GAAGA 3 cut(s) 228, 415, 667
MfeI CAATTG 2 cut(s) 384, 390
MflI RGATCY 1 cut(s) 375
MhlI GDGCHC 3 cut(s) 752, 801, 1051
MlsI TGGCCA 1 cut(s) 472
MluCI AATT 8 cut(s) 109, 124, 170, 341, 384, 390, 415, 856
MluNI TGGCCA 1 cut(s) 472
MmeI TCCRAC 1 cut(s) 830
MnlI CCTC 9 cut(s) 164, 246, 317, 421, 583, 762, 1108, 1111, 1140
Mox20I TGGCCA 1 cut(s) 472
Mph1103I ATGCAT 1 cut(s) 1006
MscI TGGCCA 1 cut(s) 472
MseI TTAA 2 cut(s) 147, 344
MslI CAYNNNNRTG 2 cut(s) 615, 979
Msp20I TGGCCA 1 cut(s) 472
MspA1I CMGCKG 1 cut(s) 453
MspI CCGG 2 cut(s) 766, 979
MspR9I CCNGG 1 cut(s) 843
MunI CAATTG 2 cut(s) 384, 390
MvaI CCWGG 1 cut(s) 843
MwoI GCNNNNNNNGC 6 cut(s) 264, 395, 829, 852, 970, 1046
NcoI CCATGG 1 cut(s) 473
NdeII GATC 2 cut(s) 375, 1096
NheI GCTAGC 1 cut(s) 805
NlaIII CATG 3 cut(s) 477, 731, 793
NlaIV GGNNCC 2 cut(s) 764, 948
NmuCI GTSAC 2 cut(s) 12, 883
NsiI ATGCAT 1 cut(s) 1006
NspV TTCGAA 1 cut(s) 520
PaqCI CACCTGC 1 cut(s) 613
PceI AGGCCT 1 cut(s) 498
PfeI GAWTC 2 cut(s) 272, 317
PflMI CCANNNNNTGG 1 cut(s) 405
PinAI ACCGGT 1 cut(s) 978
PkrI GCNGC 4 cut(s) 397, 558, 811, 831
Psp1406I AACGTT 1 cut(s) 697
Psp6I CCWGG 1 cut(s) 841
PspGI CCWGG 1 cut(s) 841
PspN4I GGNNCC 2 cut(s) 764, 948
PspPI GGNCC 3 cut(s) 408, 821, 971
PsuI RGATCY 1 cut(s) 375
PvuII CAGCTG 1 cut(s) 453
RsaI GTAC 4 cut(s) 446, 659, 764, 1091
RsaNI GTAC 4 cut(s) 445, 658, 763, 1090
RseI CAYNNNNRTG 2 cut(s) 615, 979
SaqAI TTAA 2 cut(s) 147, 344
SatI GCNGC 4 cut(s) 396, 557, 810, 830
Sau3AI GATC 2 cut(s) 375, 1096
Sau96I GGNCC 3 cut(s) 408, 821, 971
ScrFI CCNGG 1 cut(s) 843
SduI GDGCHC 3 cut(s) 752, 801, 1051
SfaNI GCATC 1 cut(s) 1013
SfuI TTCGAA 1 cut(s) 520
SinI GGWCC 1 cut(s) 408
SmiMI CAYNNNNRTG 2 cut(s) 615, 979
Sse9I AATT 8 cut(s) 109, 124, 170, 341, 384, 390, 415, 856
SseBI AGGCCT 1 cut(s) 498
SsiI CCGC 1 cut(s) 846
SspMI CTAG 1 cut(s) 806
StuI AGGCCT 1 cut(s) 498
StyD4I CCNGG 1 cut(s) 841
StyI CCWWGG 1 cut(s) 473
TaaI ACNGT 2 cut(s) 515, 836
TaiI ACGT 1 cut(s) 700
TaqI TCGA 2 cut(s) 520, 1045
TasI AATT 8 cut(s) 109, 124, 170, 341, 384, 390, 415, 856
TatI WGTACW 2 cut(s) 444, 1089
TfiI GAWTC 2 cut(s) 272, 317
Tru1I TTAA 2 cut(s) 147, 344
Tru9I TTAA 2 cut(s) 147, 344
TscAI CASTG 3 cut(s) 582, 784, 806
TseFI GTSAC 2 cut(s) 12, 883
TseI GCWGC 4 cut(s) 395, 556, 809, 829
Tsp45I GTSAC 2 cut(s) 12, 883
TspDTI ATGAA 3 cut(s) 309, 1057, 1174
TspRI CASTG 3 cut(s) 582, 784, 806
Van91I CCANNNNNTGG 1 cut(s) 405
VpaK11BI GGWCC 1 cut(s) 408
XspI CTAG 1 cut(s) 806
Zsp2I ATGCAT 1 cut(s) 1006
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.