AT5G59750

GTP cyclohydrolase II

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0011803
Physical Location & Seq
Forward (+)
24073195 .. 24075552
2358 bp
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UTR
Exon/CDS
Intron
AT5G59750.3

Sequence Viewer

Length: 1530 bp
ATGATGGATTCTGCTTTATATCATCCTCGAATCTTTTTCGCTCACAGCTTCATCAATGGCTTGTATAGTTCTCCTAGGTTTGCTAATACTTGCTGGAGATTGGTTAGTCGTTCAAGTTGGGAGATCAAGGCTAGTGAGAATAGTGATCGCAATGTGTTCGATGAAAATCCTGTTAGGAAAACCGACGGGTCGTTGTTTGATTCTGCTTCGTTTGAGACAGTTGACGCGGAAATCACGCCTGAGACTGATGATTTCTTCGTGAGTGACGCAGAGGGTGATCCAGATTGTCCTACTCAAGGTTATTCCTCTATTGAACTTGCTCTTCAAGCTCTACGCAAAGGAAAGTTTGTGATCGTTGTTGACGATGAAACCGGAGATGTTGAAGGGAATCTGATCATGGCTGCAACTCTTACGAGTCCAAAAGACATAGCTTTTCTGATTAAGAATGGTTCAGGTATTGTCTCAGTTGGTATGAAGAAAGAAAACCTTGAAAGATTAAGCCTTACACTTATGTCACCTGAAATGGAAGACGAAGATTCCTCTGCTCCAACTTTCACCATCACTGTGGATGCAAAATCTGGAACATCAACTGGAGTATCAGCTTCAGACAGGGCGATGACGGTTCTTGCACTTTCGTCTCTCGATGCTAAACCAGATGATTTCAGGAGGCCTGGTCATGTATTCCCTCTCAAGTACAGAGATGGTGGAGTTTTAAGGAGAGCTGGTCACACCGAAGCTTCAGTGGATCTAATGATATTGGCTGGTTTACGTCCCCTCTCTGTTCTTTCAGCTATTCTTGATCAAGAAGATGGGTCTATGGCTTCACTGCCGTATATGAAAAAGCTTGCTACGGAACATGATATCCCCATTGTATCGTTGACTGATTTAATCAGATATCGAAGAAAGCGAGATAAGCTTGTTGAGAGAATCACAGTGTCTCGTTTGCCTACCAAATGGGGTCTTTTCCAAGCTTATTGTTATCGATCTAAACTCGATGGAACCGAAAATATCGCCCTCGTTAAGGGAAACGTTGGAAATGGTGAGGACATTCTGGTGCGAGTGCATTCAGAGTGCTTGACAGGAGACATTTTTGGCTCAGCACGGTGTGACTGTGGAAACCAATTGGACTTAGCCATGGAGTTAATCGAGAAAGAGGGAAGAGGAGTTGTTGTGTATCTCCGTGGCCACGAAGGAAGAGGCATTGGCCTTGGTCACAAGCTTAGAGCTTATAACTTGCAGGACGAAGGACACGACACTGTTCAGGCCAATGTTGAACTTGGCCTATCCATTGATTCTCGTGAATACGGTATCGGTGCTCAGATGCTACGAGACATAGGAGTGAGAACAATGAGACTAATGACGAATAACCCTGCCAAATTCACTGGTCTGAAAGGGTACGGACTCGCTGTAGTTGGCCGTGTGCCGGTGGTGACACCAATCACCAAGGAGAACAGAAGATATATGGAGACCAAACGCAAGAAGATGGGTCACATTTATATCTCTGATAACAACGATCAGCCATTAGCTTGA

Protein Analysis

509

Amino Acids

56.15

Weight (kDa)

5.7

Isoelectric Point (pI)

36.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHBP_synthase PF00926 107 - 299 1.3e-67 3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2 PF00925 311 - 441 1.9e-52 GTP cyclohydrolase II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59750 AT5G59750 AT5G59750
fragaria_vesca FvH4_3g34860 FvH4_3g34860
malus_domestica MD03G1100600.v1.1
prunus_persica Prupe.6G085600_v2.0.a1 Prupe.6G085600_v2.0.a1
pyrus_communis pycom03g08270
rosa_chinensis RchiOBHm_Chr5g0062711
rosa_laevigata RLG00000035574
rosa_multiflora Rmu_ssc0000472.1_g000019
rosa_roxburghii Rroxscaffold_1G00017910
rosa_rugosa Rorug05G0351100
rosa_samantha Rh5AG412100 Rh5BG426100 Rh5CG450200 Rh5DG440100
rosa_wichuraiana Rw5G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
AccII CGCG 1 cut(s) 227
AciI CCGC 1 cut(s) 227
AclI AACGTT 1 cut(s) 1029
AclWI GGATC 2 cut(s) 272, 753
AcoI YGGCCR 2 cut(s) 1183, 1414
AcsI RAATTY 1 cut(s) 1376
AcuI CTGAAG 2 cut(s) 588, 723
AdeI CACNNNGTG 1 cut(s) 1106
AfaI GTAC 2 cut(s) 695, 1397
AfiI CCNNNNNNNGG 3 cut(s) 296, 1021, 1423
AgsI TTSAA 6 cut(s) 114, 314, 326, 383, 491, 1274
AjnI CCWGG 1 cut(s) 670
AloI GAACNNNNNNTCC 2 cut(s) 846, 878
Alw21I GWGCWC 1 cut(s) 1318
Alw26I GTCTC 9 cut(s) 209, 236, 466, 642, 942, 1077, 1323, 1345, 1460
AlwI GGATC 2 cut(s) 272, 753
AoxI GGCC 6 cut(s) 668, 1183, 1204, 1263, 1279, 1414
ApeKI GCWGC 1 cut(s) 401
ApoI RAATTY 1 cut(s) 1376
AspA2I CCTAGG 1 cut(s) 74
AsuHPI GGTGA 6 cut(s) 287, 507, 547, 1052, 1432, 1441
AvrII CCTAGG 1 cut(s) 74
BaeI ACNNNNGTAYC 2 cut(s) 579, 612
BalI TGGCCA 1 cut(s) 1185
BauI CACGAG 1 cut(s) 1296
BbsI GAAGAC 1 cut(s) 534
Bbv12I GWGCWC 1 cut(s) 1318
BbvI GCAGC 1 cut(s) 388
BccI CCATC 5 cut(s) 566, 695, 803, 989, 1477
BceAI ACGGC 2 cut(s) 814, 1401
BcgI CGANNNNNNTGC 2 cut(s) 139, 173
BciT130I CCWGG 1 cut(s) 672
BclI TGATCA 2 cut(s) 393, 799
BcoDI GTCTC 9 cut(s) 209, 236, 466, 642, 942, 1077, 1323, 1345, 1460
BfaI CTAG 2 cut(s) 75, 132
BfmI CTRYAG 1 cut(s) 1407
BisI GCNGC 1 cut(s) 402
BlnI CCTAGG 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 1096
BlsI GCNGC 1 cut(s) 403
Bme1390I CCNGG 1 cut(s) 672
BmiI GGNNCC 1 cut(s) 1000
BmrFI CCNGG 1 cut(s) 672
BmsI GCATC 3 cut(s) 559, 634, 1311
BpiI GAAGAC 1 cut(s) 534
BpmI CTGGAG 2 cut(s) 115, 612
Bpu1102I GCTNAGC 1 cut(s) 1096
BpuEI CTTGAG 2 cut(s) 279, 674
Bsa29I ATCGAT 1 cut(s) 982
BsaBI GATNNNNATC 1 cut(s) 165
BsaI GGTCTC 1 cut(s) 1460
BsaJI CCNNGG 5 cut(s) 74, 1134, 1180, 1207, 1443
BsaWI WCCGGW 1 cut(s) 371
BsaXI ACNNNNNCTCC 6 cut(s) 658, 688, 709, 739, 1155, 1185
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 1021, 1423
Bse118I RCCGGY 1 cut(s) 1423
Bse1I ACTGG 2 cut(s) 595, 1387
Bse3DI GCAATG 1 cut(s) 157
Bse8I GATNNNNATC 1 cut(s) 165
BseBI CCWGG 1 cut(s) 672
BseCI ATCGAT 1 cut(s) 982
BseDI CCNNGG 5 cut(s) 74, 1134, 1180, 1207, 1443
BseGI GGATG 2 cut(s) 22, 574
BseJI GATNNNNATC 1 cut(s) 165
BseLI CCNNNNNNNGG 3 cut(s) 296, 1021, 1423
BseMI GCAATG 1 cut(s) 157
BseMII CTCAG 4 cut(s) 231, 477, 1110, 1331
BseNI ACTGG 2 cut(s) 595, 1387
BseRI GAGGAG 1 cut(s) 1176
BseXI GCAGC 1 cut(s) 388
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 6 cut(s) 670, 1185, 1206, 1265, 1281, 1416
BshVI ATCGAT 1 cut(s) 982
BsiHKAI GWGCWC 1 cut(s) 1318
BsiSI CCGG 2 cut(s) 372, 1424
BslFI GGGAC 1 cut(s) 756
BslI CCNNNNNNNGG 3 cut(s) 296, 1021, 1423
BsmAI GTCTC 9 cut(s) 209, 236, 466, 642, 942, 1077, 1323, 1345, 1460
BsmBI CGTCTC 1 cut(s) 642
BsmFI GGGAC 1 cut(s) 756
BsmI GAATGC 1 cut(s) 1063
BsnI GGCC 6 cut(s) 670, 1185, 1206, 1265, 1281, 1416
Bso31I GGTCTC 1 cut(s) 1460
Bsp1286I GDGCHC 1 cut(s) 1318
Bsp143I GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
Bsp1720I GCTNAGC 1 cut(s) 1096
Bsp19I CCATGG 1 cut(s) 1134
BspACI CCGC 1 cut(s) 227
BspANI GGCC 6 cut(s) 670, 1185, 1206, 1265, 1281, 1416
BspCNI CTCAG 4 cut(s) 232, 476, 1109, 1330
BspDI ATCGAT 1 cut(s) 982
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 1 cut(s) 1000
BspPI GGATC 2 cut(s) 272, 753
BspQI GCTCTTC 1 cut(s) 327
BspTNI GGTCTC 1 cut(s) 1460
BsrDI GCAATG 1 cut(s) 157
BsrFI RCCGGY 1 cut(s) 1423
BsrI ACTGG 2 cut(s) 595, 1387
BssAI RCCGGY 1 cut(s) 1423
BssECI CCNNGG 5 cut(s) 74, 1134, 1180, 1207, 1443
BssMI GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
BssSI CACGAG 1 cut(s) 1296
BssT1I CCWWGG 4 cut(s) 74, 1134, 1207, 1443
Bst2BI CACGAG 1 cut(s) 1296
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 8 cut(s) 220, 565, 622, 934, 1104, 1112, 1258, 1307
Bst6I CTCTTC 3 cut(s) 327, 1153, 1189
BstC8I GCNNGC 1 cut(s) 846
BstDEI CTNAG 6 cut(s) 240, 463, 1096, 1129, 1220, 1317
BstDSI CCRYGG 2 cut(s) 1134, 1180
BstENI CCTNNNNNAGG 2 cut(s) 294, 1019
BstF5I GGATG 2 cut(s) 22, 574
BstFNI CGCG 1 cut(s) 227
BstKTI GATC 9 cut(s) 126, 148, 280, 354, 396, 748, 802, 986, 1516
BstMAI GTCTC 9 cut(s) 209, 236, 466, 642, 942, 1077, 1323, 1345, 1460
BstMBI GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
BstMWI GCNNNNNNNGC 2 cut(s) 326, 913
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 670
BstSFI CTRYAG 1 cut(s) 1407
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 534
BstX2I RGATCY 1 cut(s) 745
BstXI CCANNNNNNTGG 1 cut(s) 565
BstYI RGATCY 1 cut(s) 745
Bsu15I ATCGAT 1 cut(s) 982
BsuRI GGCC 6 cut(s) 670, 1185, 1206, 1265, 1281, 1416
BsuTUI ATCGAT 1 cut(s) 982
BtgI CCRYGG 2 cut(s) 1134, 1180
BtgZI GCGATG 1 cut(s) 629
BtsCI GGATG 2 cut(s) 22, 574
BtsI GCAGTG 1 cut(s) 824
BtsIMutI CAGTG 6 cut(s) 561, 747, 824, 939, 1254, 1380
Cac8I GCNNGC 1 cut(s) 846
Cfr10I RCCGGY 1 cut(s) 1423
ClaI ATCGAT 1 cut(s) 982
CseI GACGC 2 cut(s) 233, 275
Csp6I GTAC 2 cut(s) 694, 1396
CviAII CATG 4 cut(s) 397, 677, 857, 1135
CviQI GTAC 2 cut(s) 694, 1396
DdeI CTNAG 6 cut(s) 240, 463, 1096, 1129, 1220, 1317
DpnI GATC 9 cut(s) 125, 147, 279, 353, 395, 747, 801, 985, 1515
DpnII GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
DraIII CACNNNGTG 1 cut(s) 1106
EaeI YGGCCR 2 cut(s) 1183, 1414
Eam1104I CTCTTC 3 cut(s) 327, 1153, 1189
EarI CTCTTC 3 cut(s) 327, 1153, 1189
Eco130I CCWWGG 4 cut(s) 74, 1134, 1207, 1443
Eco147I AGGCCT 1 cut(s) 670
Eco31I GGTCTC 1 cut(s) 1460
Eco32I GATATC 2 cut(s) 862, 896
Eco57I CTGAAG 2 cut(s) 588, 723
EcoNI CCTNNNNNAGG 2 cut(s) 294, 1019
EcoRII CCWGG 1 cut(s) 670
EcoRV GATATC 2 cut(s) 862, 896
EcoT14I CCWWGG 4 cut(s) 74, 1134, 1207, 1443
ErhI CCWWGG 4 cut(s) 74, 1134, 1207, 1443
Esp3I CGTCTC 1 cut(s) 642
FaeI CATG 4 cut(s) 400, 680, 860, 1138
FalI AAGNNNNNCTT 2 cut(s) 471, 503
FaqI GGGAC 1 cut(s) 756
FatI CATG 4 cut(s) 396, 676, 856, 1134
FbaI TGATCA 2 cut(s) 393, 799
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 2 cut(s) 9, 581
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 2 cut(s) 75, 132
GluI GCNGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 115, 612
HaeIII GGCC 6 cut(s) 670, 1185, 1206, 1265, 1281, 1416
HapII CCGG 2 cut(s) 372, 1424
HgaI GACGC 2 cut(s) 233, 275
Hin1II CATG 4 cut(s) 400, 680, 860, 1138
HincII GTYRAC 3 cut(s) 223, 361, 879
HindII GTYRAC 3 cut(s) 223, 361, 879
HindIII AAGCTT 5 cut(s) 735, 842, 914, 969, 1217
HinfI GANTC 9 cut(s) 8, 30, 200, 388, 415, 536, 927, 1292, 1401
HpaII CCGG 2 cut(s) 372, 1424
HphI GGTGA 6 cut(s) 287, 507, 547, 1052, 1432, 1441
Hpy166II GTNNAC 4 cut(s) 223, 361, 767, 879
Hpy188I TCNGA 8 cut(s) 393, 438, 607, 893, 1069, 1320, 1389, 1504
Hpy188III TCNNGA 9 cut(s) 259, 281, 579, 641, 664, 797, 803, 1147, 1298
Hpy8I GTNNAC 4 cut(s) 223, 361, 767, 879
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 3 cut(s) 377, 1184, 1238
HpyCH4III ACNGT 8 cut(s) 220, 565, 622, 934, 1104, 1112, 1258, 1307
HpyCH4IV ACGT 2 cut(s) 769, 1029
HpyCH4V TGCA 5 cut(s) 404, 572, 629, 1063, 1237
HpyF10VI GCNNNNNNNGC 2 cut(s) 326, 913
HpyF3I CTNAG 6 cut(s) 240, 463, 1096, 1129, 1220, 1317
HpySE526I ACGT 2 cut(s) 769, 1029
Hsp92II CATG 4 cut(s) 400, 680, 860, 1138
Ksp22I TGATCA 2 cut(s) 393, 799
Kzo9I GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
LguI GCTCTTC 1 cut(s) 327
LmnI GCTCC 1 cut(s) 550
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 3 cut(s) 559, 634, 1311
MaeI CTAG 2 cut(s) 75, 132
MaeII ACGT 2 cut(s) 769, 1029
MaeIII GTNAC 7 cut(s) 263, 513, 725, 1106, 1211, 1429, 1487
MalI GATC 9 cut(s) 125, 147, 279, 353, 395, 747, 801, 985, 1515
MboI GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
MfeI CAATTG 1 cut(s) 1121
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 1 cut(s) 1318
MlsI TGGCCA 1 cut(s) 1185
MluCI AATT 2 cut(s) 1121, 1376
MluNI TGGCCA 1 cut(s) 1185
MlyI GAGTC 2 cut(s) 424, 1395
MmeI TCCRAC 2 cut(s) 572, 1012
Mox20I TGGCCA 1 cut(s) 1185
MscI TGGCCA 1 cut(s) 1185
MseI TTAA 6 cut(s) 441, 497, 713, 887, 1020, 1142
MslI CAYNNNNRTG 3 cut(s) 563, 1052, 1337
Msp20I TGGCCA 1 cut(s) 1185
MspI CCGG 2 cut(s) 372, 1424
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 1 cut(s) 1121
Mva1269I GAATGC 1 cut(s) 1063
MvaI CCWGG 1 cut(s) 672
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 2 cut(s) 326, 913
NcoI CCATGG 1 cut(s) 1134
NdeII GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
NlaIII CATG 4 cut(s) 400, 680, 860, 1138
NlaIV GGNNCC 1 cut(s) 1000
NmuCI GTSAC 7 cut(s) 263, 513, 725, 1106, 1211, 1429, 1487
PceI AGGCCT 1 cut(s) 670
PciSI GCTCTTC 1 cut(s) 327
PcsI WCGNNNNNNNCGW 4 cut(s) 360, 904, 999, 1248
PctI GAATGC 1 cut(s) 1063
PfeI GAWTC 7 cut(s) 8, 30, 200, 388, 536, 927, 1292
PkrI GCNGC 1 cut(s) 403
PleI GAGTC 2 cut(s) 423, 1395
PpsI GAGTC 2 cut(s) 423, 1395
PsiI TTATAA 1 cut(s) 1230
Psp1406I AACGTT 1 cut(s) 1029
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 1 cut(s) 1000
PsuI RGATCY 1 cut(s) 745
RsaI GTAC 2 cut(s) 695, 1397
RsaNI GTAC 2 cut(s) 694, 1396
RseI CAYNNNNRTG 3 cut(s) 563, 1052, 1337
SapI GCTCTTC 1 cut(s) 327
SaqAI TTAA 6 cut(s) 441, 497, 713, 887, 1020, 1142
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 9 cut(s) 123, 145, 277, 351, 393, 745, 799, 983, 1513
SchI GAGTC 2 cut(s) 424, 1395
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 1 cut(s) 1318
SfaNI GCATC 3 cut(s) 559, 634, 1311
SfcI CTRYAG 1 cut(s) 1407
SmiMI CAYNNNNRTG 3 cut(s) 563, 1052, 1337
SmlI CTYRAG 2 cut(s) 294, 689
SmoI CTYRAG 2 cut(s) 294, 689
Sse9I AATT 2 cut(s) 1121, 1376
SseBI AGGCCT 1 cut(s) 670
SsiI CCGC 1 cut(s) 227
SspMI CTAG 2 cut(s) 75, 132
StuI AGGCCT 1 cut(s) 670
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 4 cut(s) 74, 1134, 1207, 1443
TaaI ACNGT 8 cut(s) 220, 565, 622, 934, 1104, 1112, 1258, 1307
TaiI ACGT 2 cut(s) 772, 1032
TaqI TCGA 7 cut(s) 28, 159, 642, 898, 982, 993, 1146
TasI AATT 2 cut(s) 1121, 1376
TatI WGTACW 1 cut(s) 693
TfiI GAWTC 7 cut(s) 8, 30, 200, 388, 536, 927, 1292
Tru1I TTAA 6 cut(s) 441, 497, 713, 887, 1020, 1142
Tru9I TTAA 6 cut(s) 441, 497, 713, 887, 1020, 1142
TscAI CASTG 6 cut(s) 568, 747, 831, 939, 1261, 1387
TseFI GTSAC 7 cut(s) 263, 513, 725, 1106, 1211, 1429, 1487
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 7 cut(s) 263, 513, 725, 1106, 1211, 1429, 1487
TspDTI ATGAA 5 cut(s) 40, 177, 381, 488, 851
TspGWI ACGGA 3 cut(s) 866, 1169, 1413
TspRI CASTG 6 cut(s) 568, 747, 831, 939, 1261, 1387
XagI CCTNNNNNAGG 2 cut(s) 294, 1019
XapI RAATTY 1 cut(s) 1376
XmaJI CCTAGG 1 cut(s) 74
XspI CTAG 2 cut(s) 75, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.