Rw5G038740

riboflavin biosynthesis protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011803
Physical Location & Seq
Forward (+)
68482502 .. 68485758
3257 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G038740.1

Sequence Viewer

Length: 1638 bp
ATGCATATTCTGTTAATCGAATCATCAGCATCAACGTCCTATATCACTAATTTGATTCATCTACCTAAAAATTTCAAACAACTTAATTTTTTTTTGCATCGATATTTTGCGGCTCCTCAGAAGCAAAGGTGGTTGAATCCAAGTTGTTGGGCTATTGGGGTTTCTAGAACTGGAGAGGGAAACCTTTCTAATGATATTCCTTTAAAGAAAAGTGAAAATGGGTCTTTGTTGGGTGGCTTGGACGAGTCAGCTTCATCAGCACCAATAATTGGGACATTGGATGCTGAGATTACACCAGAGACCATTGATTTCTTTGTTAGTGATGCCGAGGGTGATCCTGATTCTCCATCGGAGGGCTACTCGTCCATAGAGCAAGCGCTCAATACATTACGCCAAGGAAAGTTTGTGATTGTTGTAGATGATGAAAATGCGGAAGTTGAAGGAAACCTTGTCATGGCAGCATCATTTGCAAATCCTAGAAACATAGCATTCCTAATTAAACACGGATCTGGGATCGTCTCTGTAGGCATGAAGGAGGAGGATCTTGAAAGATTGAAGCTTCCTCTGATGTCACCAGAGACCGAAGACGAAGACTCTTCTGCCCCCACATTCACAATCACAGTGGATGCAAAAATTGGAACATCTACTGGAGTATCAGCTCCAGACAGGGCAAAGACTGTTCTTGCTCTTTCATCTAAGGAGTCAAAGCCGGAAGATTTTAGAAGACCTGGCCATGTGTTTCCCCTCAAGTACCGCAATGGTGGGGTTCTTCGGAGAGCTGGTCACACTGAGGCTTCTGTTGATCTGGTTGTTCTGGCGGGCTTGAGGCCAGTGTCTGTTCTTTCAGCCGTAGTTGATGCAGAAGATGGCTCTATGGCGTCTTTGCCCGATTTAAAAAAACTGTCATTGGACCACAGCATTTCAATTGTCTCAATAACTGATCTGATTAGGTACAGGAGAAAGAGGGAAAAGATAGTTGAAAGAACTGCTCTATCGCGTTTGCCTACAAAATGGGGTCTGTTTCAGGCTTATTGCTACCGCTCAAAGCTAGACGGAACAGAACATGTTGCTGTTGTGAAAGGAAGCATAGGAAATGGAGAAGATGTGCTAGTAAGAGTTCATTCAGAGTGTTTGACAGGTGACATTTTTGGGTCTGGGCGGTGTGATTGTGGCAACCAGTTGGAATTGGCAATGCAGCTAATCGAACAGGCTGGTCGAGGTGCTGTTGTTTACCTTCGAGGTCATGAAGGAAGAGGCATTGGTCTTGGTCACAAACTTCAAGCCTACAATCTTCAAGACCAAGGCCATGACACAGTCCAGGCTAATATAGAACTTGGACTAGCTGTCGATGCTCGCGAATATGGAATTGGTGCACAGATTCTAAGAGACATAGGAGTTCGAACGATGAGGCTGATGACAAACAATCCAGCCAAGTTCAGTGGTCTGAAGGGTTATGGTTTGGCTGTGATTGGTAGGGTTCCGGTGTTAACACCAATTACAGAGGAAAACAAGAAGTACTTGGAAACAAAAAGAACTAAGATGGGGCATGTTTATGGTTCTGATATCCAAGGATCTTCAGCCGGTTTCATCAAACCAAATGTATATAACAAGGACATAGAAAACAAACCTGAAAGCTAA

Protein Analysis

545

Amino Acids

59.29

Weight (kDa)

6.16

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DHBP_synthase PF00926 124 - 318 8.9e-71 3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2 PF00925 330 - 494 2.2e-71 GTP cyclohydrolase II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011803)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59750 AT5G59750 AT5G59750
fragaria_vesca FvH4_3g34860 FvH4_3g34860
malus_domestica MD03G1100600.v1.1
prunus_persica Prupe.6G085600_v2.0.a1 Prupe.6G085600_v2.0.a1
pyrus_communis pycom03g08270
rosa_chinensis RchiOBHm_Chr5g0062711
rosa_laevigata RLG00000035574
rosa_multiflora Rmu_ssc0000472.1_g000019
rosa_roxburghii Rroxscaffold_1G00017910
rosa_rugosa Rorug05G0351100
rosa_samantha Rh5AG412100 Rh5BG426100 Rh5CG450200 Rh5DG440100
rosa_wichuraiana Rw5G038740

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 1041
AccII CGCG 2 cut(s) 997, 1356
AciI CCGC 6 cut(s) 110, 431, 754, 818, 1039, 1159
AclWI GGATC 5 cut(s) 329, 514, 521, 549, 1579
AcoI YGGCCR 1 cut(s) 730
AcsI RAATTY 1 cut(s) 70
AcuI CTGAAG 2 cut(s) 1466, 1560
AcyI GRCGYC 1 cut(s) 878
AfaI GTAC 3 cut(s) 752, 953, 1517
AfeI AGCGCT 1 cut(s) 378
AfiI CCNNNNNNNGG 3 cut(s) 269, 353, 454
AflIII ACRYGT 1 cut(s) 1063
AgsI TTSAA 9 cut(s) 76, 136, 440, 548, 556, 924, 980, 1280, 1295
AhdI GACNNNNNGTC 1 cut(s) 1343
AjnI CCWGG 2 cut(s) 727, 1317
AjuI GAANNNNNNNTTGG 2 cut(s) 1350, 1382
AluBI AGCT 8 cut(s) 251, 559, 659, 779, 1048, 1198, 1343, 1635
AluI AGCT 8 cut(s) 251, 559, 659, 779, 1048, 1198, 1343, 1635
Alw21I GWGCWC 1 cut(s) 1375
Alw26I GTCTC 5 cut(s) 293, 523, 572, 934, 1380
Alw44I GTGCAC 1 cut(s) 1371
AlwI GGATC 5 cut(s) 329, 514, 521, 549, 1579
AlwNI CAGNNNCTG 1 cut(s) 836
Aor51HI AGCGCT 1 cut(s) 378
AoxI GGCC 3 cut(s) 730, 827, 1303
ApaLI GTGCAC 1 cut(s) 1371
ApeKI GCWGC 2 cut(s) 458, 1195
ApoI RAATTY 1 cut(s) 70
Asp700I GAANNNNTTC 1 cut(s) 184
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 1 cut(s) 910
AsuHPI GGTGA 3 cut(s) 344, 564, 1151
AsuII TTCGAA 1 cut(s) 1399
AvaII GGWCC 1 cut(s) 910
BaeGI GKGCMC 1 cut(s) 1375
BaeI ACNNNNGTAYC 2 cut(s) 636, 669
BalI TGGCCA 1 cut(s) 732
BbsI GAAGAC 3 cut(s) 591, 597, 730
Bbv12I GWGCWC 1 cut(s) 1375
BbvI GCAGC 2 cut(s) 470, 1207
BccI CCATC 3 cut(s) 355, 860, 1534
BceAI ACGGC 1 cut(s) 833
BciT130I CCWGG 2 cut(s) 729, 1319
BcoDI GTCTC 5 cut(s) 293, 523, 572, 934, 1380
BfaI CTAG 5 cut(s) 165, 477, 1049, 1109, 1340
BfmI CTRYAG 1 cut(s) 522
BfoI RGCGCY 1 cut(s) 380
BisI GCNGC 3 cut(s) 111, 459, 1196
BlsI GCNGC 3 cut(s) 112, 460, 1197
BmcAI AGTACT 1 cut(s) 1517
Bme1390I CCNGG 2 cut(s) 729, 1319
Bme18I GGWCC 1 cut(s) 910
BmeRI GACNNNNNGTC 1 cut(s) 1343
BmgT120I GGNCC 1 cut(s) 910
BmiI GGNNCC 2 cut(s) 114, 1479
BmrFI CCNGG 2 cut(s) 729, 1319
BmsI GCATC 8 cut(s) 38, 106, 271, 313, 470, 616, 847, 1339
BpiI GAAGAC 3 cut(s) 591, 597, 730
BplI GAGNNNNNCTC 2 cut(s) 344, 376
BpmI CTGGAG 3 cut(s) 192, 645, 669
Bpu14I TTCGAA 1 cut(s) 1399
BpuEI CTTGAG 2 cut(s) 731, 844
Bsa29I ATCGAT 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 878
BsaI GGTCTC 2 cut(s) 293, 572
BsaJI CCNNGG 4 cut(s) 327, 394, 1300, 1567
BsaWI WCCGGW 1 cut(s) 1480
BsaXI ACNNNNNCTCC 4 cut(s) 766, 796, 1089, 1119
Bsc4I CCNNNNNNNGG 3 cut(s) 269, 353, 454
Bse118I RCCGGY 1 cut(s) 1580
Bse1I ACTGG 4 cut(s) 175, 652, 830, 1177
Bse3DI GCAATG 2 cut(s) 763, 1197
BseBI CCWGG 2 cut(s) 729, 1319
BseCI ATCGAT 1 cut(s) 100
BseDI CCNNGG 4 cut(s) 327, 394, 1300, 1567
BseGI GGATG 2 cut(s) 286, 631
BseLI CCNNNNNNNGG 3 cut(s) 269, 353, 454
BseMI GCAATG 2 cut(s) 763, 1197
BseMII CTCAG 3 cut(s) 131, 276, 780
BseNI ACTGG 4 cut(s) 175, 652, 830, 1177
BseRI GAGGAG 2 cut(s) 105, 551
BseSI GKGCMC 1 cut(s) 1375
BseXI GCAGC 2 cut(s) 470, 1207
Bsh1236I CGCG 2 cut(s) 997, 1356
BshFI GGCC 3 cut(s) 732, 829, 1305
BshVI ATCGAT 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 1375
BsiSI CCGG 3 cut(s) 710, 1481, 1581
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 3 cut(s) 269, 353, 454
BsmAI GTCTC 5 cut(s) 293, 523, 572, 934, 1380
BsmBI CGTCTC 1 cut(s) 523
BsmFI GGGAC 1 cut(s) 286
BsmI GAATGC 1 cut(s) 488
BsnI GGCC 3 cut(s) 732, 829, 1305
Bso31I GGTCTC 2 cut(s) 293, 572
Bsp119I TTCGAA 1 cut(s) 1399
Bsp1286I GDGCHC 1 cut(s) 1375
Bsp143I GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
Bsp68I TCGCGA 1 cut(s) 1356
BspACI CCGC 6 cut(s) 110, 431, 754, 818, 1039, 1159
BspANI GGCC 3 cut(s) 732, 829, 1305
BspCNI CTCAG 3 cut(s) 130, 277, 781
BspDI ATCGAT 1 cut(s) 100
BspFNI CGCG 2 cut(s) 997, 1356
BspHI TCATGA 1 cut(s) 1243
BspLI GGNNCC 2 cut(s) 114, 1479
BspPI GGATC 5 cut(s) 329, 514, 521, 549, 1579
BspT104I TTCGAA 1 cut(s) 1399
BspTNI GGTCTC 2 cut(s) 293, 572
BsrBI CCGCTC 1 cut(s) 1041
BsrDI GCAATG 2 cut(s) 763, 1197
BsrFI RCCGGY 1 cut(s) 1580
BsrI ACTGG 4 cut(s) 175, 652, 830, 1177
BssAI RCCGGY 1 cut(s) 1580
BssECI CCNNGG 4 cut(s) 327, 394, 1300, 1567
BssMI GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
BssNI GRCGYC 1 cut(s) 878
BssT1I CCWWGG 3 cut(s) 394, 1300, 1567
Bst2UI CCWGG 2 cut(s) 729, 1319
Bst4CI ACNGT 4 cut(s) 622, 679, 903, 1315
Bst6I CTCTTC 2 cut(s) 601, 1246
BstACI GRCGYC 1 cut(s) 878
BstAPI GCANNNNNTGC 1 cut(s) 467
BstBI TTCGAA 1 cut(s) 1399
BstC8I GCNNGC 3 cut(s) 375, 820, 1354
BstDEI CTNAG 6 cut(s) 117, 285, 696, 789, 1382, 1536
BstF5I GGATG 2 cut(s) 286, 631
BstFNI CGCG 2 cut(s) 997, 1356
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 7 cut(s) 337, 509, 516, 544, 805, 943, 1574
BstMAI GTCTC 5 cut(s) 293, 523, 572, 934, 1380
BstMBI GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
BstMWI GCNNNNNNNGC 3 cut(s) 257, 467, 1349
BstNI CCWGG 2 cut(s) 729, 1319
BstNSI RCATGY 2 cut(s) 1067, 1550
BstSCI CCNGG 2 cut(s) 727, 1317
BstSFI CTRYAG 1 cut(s) 522
BstSLI GKGCMC 1 cut(s) 1375
BstUI CGCG 2 cut(s) 997, 1356
BstV1I GCAGC 2 cut(s) 470, 1207
BstV2I GAAGAC 3 cut(s) 591, 597, 730
BstX2I RGATCY 3 cut(s) 506, 541, 1571
BstXI CCANNNNNNTGG 1 cut(s) 147
BstYI RGATCY 3 cut(s) 506, 541, 1571
Bsu15I ATCGAT 1 cut(s) 100
BsuRI GGCC 3 cut(s) 732, 829, 1305
BsuTUI ATCGAT 1 cut(s) 100
BtsCI GGATG 2 cut(s) 286, 631
BtsIMutI CAGTG 4 cut(s) 627, 786, 837, 1444
BtuMI TCGCGA 1 cut(s) 1356
Cac8I GCNNGC 3 cut(s) 375, 820, 1354
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 1 cut(s) 1243
CfoI GCGC 1 cut(s) 379
Cfr10I RCCGGY 1 cut(s) 1580
Cfr13I GGNCC 1 cut(s) 910
ClaI ATCGAT 1 cut(s) 100
CseI GACGC 1 cut(s) 867
Csp6I GTAC 3 cut(s) 751, 952, 1516
CspCI CAANNNNNGTGG 4 cut(s) 603, 638, 1420, 1455
CviAII CATG 7 cut(s) 454, 529, 734, 1064, 1244, 1307, 1547
CviQI GTAC 3 cut(s) 751, 952, 1516
DdeI CTNAG 6 cut(s) 117, 285, 696, 789, 1382, 1536
DpnI GATC 7 cut(s) 336, 508, 515, 543, 804, 942, 1573
DpnII GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
DraI TTTAAA 2 cut(s) 204, 894
DriI GACNNNNNGTC 1 cut(s) 1343
EaeI YGGCCR 1 cut(s) 730
Eam1104I CTCTTC 2 cut(s) 601, 1246
Eam1105I GACNNNNNGTC 1 cut(s) 1343
EarI CTCTTC 2 cut(s) 601, 1246
Eco130I CCWWGG 3 cut(s) 394, 1300, 1567
Eco31I GGTCTC 2 cut(s) 293, 572
Eco32I GATATC 1 cut(s) 1564
Eco47I GGWCC 1 cut(s) 910
Eco47III AGCGCT 1 cut(s) 378
Eco57I CTGAAG 2 cut(s) 1466, 1560
EcoRII CCWGG 2 cut(s) 727, 1317
EcoRV GATATC 1 cut(s) 1564
EcoT14I CCWWGG 3 cut(s) 394, 1300, 1567
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 394, 1300, 1567
Esp3I CGTCTC 1 cut(s) 523
FaeI CATG 7 cut(s) 457, 532, 737, 1067, 1247, 1310, 1550
FalI AAGNNNNNCTT 4 cut(s) 432, 464, 1502, 1534
FaqI GGGAC 1 cut(s) 286
FatI CATG 7 cut(s) 453, 528, 733, 1063, 1243, 1306, 1546
FauI CCCGC 1 cut(s) 811
Fnu4HI GCNGC 3 cut(s) 111, 459, 1196
FokI GGATG 2 cut(s) 293, 638
Fsp4HI GCNGC 3 cut(s) 111, 459, 1196
FspBI CTAG 5 cut(s) 165, 477, 1049, 1109, 1340
GlaI GCGC 1 cut(s) 378
GluI GCNGC 3 cut(s) 111, 459, 1196
GsuI CTGGAG 3 cut(s) 192, 645, 669
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 3 cut(s) 732, 829, 1305
HapII CCGG 3 cut(s) 710, 1481, 1581
HgaI GACGC 1 cut(s) 867
HhaI GCGC 1 cut(s) 379
Hin1I GRCGYC 1 cut(s) 878
Hin1II CATG 7 cut(s) 457, 532, 737, 1067, 1247, 1310, 1550
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HincII GTYRAC 1 cut(s) 1488
HindII GTYRAC 1 cut(s) 1488
HindIII AAGCTT 1 cut(s) 557
HinfI GANTC 8 cut(s) 20, 55, 136, 245, 341, 593, 701, 1378
HpaI GTTAAC 1 cut(s) 1488
HpaII CCGG 3 cut(s) 710, 1481, 1581
HphI GGTGA 3 cut(s) 344, 564, 1151
Hpy166II GTNNAC 3 cut(s) 1231, 1373, 1488
Hpy188I TCNGA 8 cut(s) 120, 352, 567, 774, 945, 1126, 1446, 1561
Hpy188III TCNNGA 7 cut(s) 165, 338, 545, 662, 1244, 1295, 1355
Hpy8I GTNNAC 3 cut(s) 1231, 1373, 1488
HpyAV CCTTC 5 cut(s) 434, 526, 1241, 1244, 1441
HpyCH4III ACNGT 4 cut(s) 622, 679, 903, 1315
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 7 cut(s) 4, 97, 470, 629, 860, 1195, 1373
HpyF10VI GCNNNNNNNGC 3 cut(s) 257, 467, 1349
HpyF3I CTNAG 6 cut(s) 117, 285, 696, 789, 1382, 1536
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 878
Hsp92II CATG 7 cut(s) 457, 532, 737, 1067, 1247, 1310, 1550
HspAI GCGC 1 cut(s) 377
KspAI GTTAAC 1 cut(s) 1488
Kzo9I GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
LmnI GCTCC 2 cut(s) 118, 664
Lsp1109I GCAGC 2 cut(s) 470, 1207
LweI GCATC 8 cut(s) 38, 106, 271, 313, 470, 616, 847, 1339
MaeI CTAG 5 cut(s) 165, 477, 1049, 1109, 1340
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 4 cut(s) 570, 782, 1139, 1268
MalI GATC 7 cut(s) 336, 508, 515, 543, 804, 942, 1573
MbiI CCGCTC 1 cut(s) 1041
MboI GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
MfeI CAATTG 1 cut(s) 924
MflI RGATCY 3 cut(s) 506, 541, 1571
MhlI GDGCHC 1 cut(s) 1375
MlsI TGGCCA 1 cut(s) 732
MluNI TGGCCA 1 cut(s) 732
MlyI GAGTC 3 cut(s) 254, 587, 710
MmeI TCCRAC 1 cut(s) 1161
Mox20I TGGCCA 1 cut(s) 732
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 184
MscI TGGCCA 1 cut(s) 732
MseI TTAA 6 cut(s) 14, 84, 203, 498, 893, 1487
MslI CAYNNNNRTG 1 cut(s) 1551
Msp20I TGGCCA 1 cut(s) 732
MspI CCGG 3 cut(s) 710, 1481, 1581
MspR9I CCNGG 2 cut(s) 729, 1319
MunI CAATTG 1 cut(s) 924
Mva1269I GAATGC 1 cut(s) 488
MvaI CCWGG 2 cut(s) 729, 1319
MvnI CGCG 2 cut(s) 997, 1356
MwoI GCNNNNNNNGC 3 cut(s) 257, 467, 1349
NdeII GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
NlaIII CATG 7 cut(s) 457, 532, 737, 1067, 1247, 1310, 1550
NlaIV GGNNCC 2 cut(s) 114, 1479
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 4 cut(s) 570, 782, 1139, 1268
NruI TCGCGA 1 cut(s) 1356
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 1067, 1550
NspV TTCGAA 1 cut(s) 1399
PagI TCATGA 1 cut(s) 1243
PciI ACATGT 1 cut(s) 1063
PcsI WCGNNNNNNNCGW 1 cut(s) 1353
PctI GAATGC 1 cut(s) 488
PdmI GAANNNNTTC 1 cut(s) 184
PfeI GAWTC 5 cut(s) 20, 55, 136, 341, 1378
PflFI GACNNNGTC 1 cut(s) 1313
PflMI CCANNNNNTGG 1 cut(s) 269
PkrI GCNGC 3 cut(s) 112, 460, 1197
PleI GAGTC 3 cut(s) 253, 587, 709
PpsI GAGTC 3 cut(s) 253, 587, 709
PscI ACATGT 1 cut(s) 1063
Psp6I CCWGG 2 cut(s) 727, 1317
PspGI CCWGG 2 cut(s) 727, 1317
PspN4I GGNNCC 2 cut(s) 114, 1479
PspPI GGNCC 1 cut(s) 910
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 3 cut(s) 506, 541, 1571
PsyI GACNNNGTC 1 cut(s) 1313
RruI TCGCGA 1 cut(s) 1356
RsaI GTAC 3 cut(s) 752, 953, 1517
RsaNI GTAC 3 cut(s) 751, 952, 1516
RseI CAYNNNNRTG 1 cut(s) 1551
SaqAI TTAA 6 cut(s) 14, 84, 203, 498, 893, 1487
SatI GCNGC 3 cut(s) 111, 459, 1196
Sau3AI GATC 7 cut(s) 334, 506, 513, 541, 802, 940, 1571
Sau96I GGNCC 1 cut(s) 910
ScaI AGTACT 1 cut(s) 1517
SchI GAGTC 3 cut(s) 254, 587, 710
ScrFI CCNGG 2 cut(s) 729, 1319
SduI GDGCHC 1 cut(s) 1375
SfaNI GCATC 8 cut(s) 38, 106, 271, 313, 470, 616, 847, 1339
SfcI CTRYAG 1 cut(s) 522
SfuI TTCGAA 1 cut(s) 1399
SinI GGWCC 1 cut(s) 910
SmiMI CAYNNNNRTG 1 cut(s) 1551
SmlI CTYRAG 2 cut(s) 746, 823
SmoI CTYRAG 2 cut(s) 746, 823
SsiI CCGC 6 cut(s) 110, 431, 754, 818, 1039, 1159
SspMI CTAG 5 cut(s) 165, 477, 1049, 1109, 1340
StyD4I CCNGG 2 cut(s) 727, 1317
StyI CCWWGG 3 cut(s) 394, 1300, 1567
TaaI ACNGT 4 cut(s) 622, 679, 903, 1315
TaiI ACGT 1 cut(s) 38
TaqI TCGA 7 cut(s) 18, 100, 1203, 1216, 1237, 1347, 1399
TaqII GACCGA 1 cut(s) 596
TatI WGTACW 1 cut(s) 1515
TauI GCSGC 1 cut(s) 113
TfiI GAWTC 5 cut(s) 20, 55, 136, 341, 1378
Tru1I TTAA 6 cut(s) 14, 84, 203, 498, 893, 1487
Tru9I TTAA 6 cut(s) 14, 84, 203, 498, 893, 1487
TscAI CASTG 4 cut(s) 627, 793, 837, 1444
TseFI GTSAC 4 cut(s) 570, 782, 1139, 1268
TseI GCWGC 2 cut(s) 458, 1195
Tsp45I GTSAC 4 cut(s) 570, 782, 1139, 1268
TspDTI ATGAA 8 cut(s) 47, 243, 438, 545, 681, 1109, 1260, 1576
TspGWI ACGGA 2 cut(s) 519, 1068
TspRI CASTG 4 cut(s) 627, 793, 837, 1444
Tth111I GACNNNGTC 1 cut(s) 1313
Van91I CCANNNNNTGG 1 cut(s) 269
VneI GTGCAC 1 cut(s) 1371
VpaK11BI GGWCC 1 cut(s) 910
XapI RAATTY 1 cut(s) 70
XbaI TCTAGA 1 cut(s) 164
XceI RCATGY 2 cut(s) 1067, 1550
XmnI GAANNNNTTC 1 cut(s) 184
XspI CTAG 5 cut(s) 165, 477, 1049, 1109, 1340
ZrmI AGTACT 1 cut(s) 1517
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.