Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0011803

Physical Location & Seq

Genomic Coordinates Forward (+)

68878910 .. 68883743

Gene Structure 4834 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000035574

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2103 bp

ATGAGCTCAGATCTGGTGTTGCTACAAGAAGCAAAACTAGTAAGCTTTCGGGGTCTGTTGTGCCATTCGTTTTCTATCGGAGCAATGACGGGGGCGTCGAATTCTTTGACAAATTACTTGGAAACGTCGCCATTTTGGAGGGCAAAATGCTCTCGGCACCCACGTCAAGAGCTGGACACCGGCGAGACGTTGAAGGTGACTACCTCCTCTCTTCTTCTCCGATTTCACTTCAATCAAGCAGCTGAATATGTAAATTCAATCTCCATATCAAAACCACAAGTCCAAGTCACCGTTGTTCATGTCTCCGTCAACCAGAACGACTCCGATTCCAATCGATGTCTCCGTCGACGACTAACTCTGATTCACCTTCGCCCACCATGGCCAGACGTTTGGATCGGAGATGACTGGTCTCTGCTTCCTGCTGCCGAGACGAGTCGAAGTTCTGATAATGCGAGAAGAGCTAGAGAAGAAGAACAAGTTCGAGTCTCTGAAAATTCTGTAATGGACTCTTCTTTATTTCCTCACCCCCTCATTCCTTACATCCTCACTAACTCGAGTTCGCATCGATATTTTGCAGCTCCTCAGAAGCAAAGGTGGTTGAATCCAAGTTGTTGGGCTATTGGGGTTTCTAGAACTGGAGAGGGAAACCTTTCTAATGATATTCCTGTAAAGAAAAGTGAAAATGGGTCTTTGTTGGGTGGCTTGGACGAGTCAGCTTCATCAGCACCAATAATTGGGACATTGGATGCTGAGATTACACCAGAGACCATTGATTTCTTTGTTAGTGATGCCGAGGGTGATCCTGATTCTCCATCGGAGGGCTACTCGTCCATAGAGCAAGCGCTCAATACATTACGCCAAGGAAAGTTTGTGATTGTTGTAGATGATGAAAATGCCGAAGTTGAAGGAAACCTTGTCATGGCAGCATCATTTGCAAATCCTAGAAACATAGCATTCCTAATTAAACACGGATCTGGGATCGTCTCTGTAGGCATGAAGGAGGAGGATCTTGAAAGATTGAAGCTTCCTCTGATGTCACCAGAGACCGAAGACGAAGACTCTTCTGCCCCCACATTCACAATCACAGTGGATGCAAAAATTGGAACATCTACTGGAGTATCAGCTCCGGACAGGGCAAAGACTGTTCTTGCTCTTTCATCTATGGAGTCAAAGCCGGAAGATTTTAGAAGGCCTGGCCATGTGTTTCCCCTCAAGTACCGCCATGGTGGGGTTCTTCGGAGAGCTGGTCACACTGAGGCTTCTGTTGATCTGGTTGTTCTGGCAGGCTTAAGGCCAGTGTCTGTTCTTTCAGCCGTAGTTGATGCAGAAGATGGCTCTATGGCGTCTTTGCCACGTTTAAAAAAACTGTCATTGGACCACAGCATTCCAATTGTCTCAATAACTGATCTGATTAGGTACAGGAGAAAGAGGGAAAAGATAGTTGAAAGAACTGCTCTATCACGTTTGCCTACAAAATGGGGTCTGTTTCAGGCTTATTGCTACCGCTCGAAGCTAGACGGAACAGAACATGTTGCTGTTGTGAAAGGAAGCATAGGAAATGGAGAAGATGTGCTAGTAAGAGTTCATTCAGAGTGTTTGACAGGTGACATTTTTGGGTCGGGGCGGTGTGATTGTGGCAACCAGTTGGAATTGGCAATGCAGCTAATCGAACAAGCTGGTCGAGGAGCCGTTGTTTACCTTCGAGGTCACGAAGGAAGAGGCATTGGTCTTGGTCACAAACTTCAAGCATATAATCTTCAAGACCAAGGCCATGACACAGTCCAGGCTAATATCGAACTTGGACTATCTATCGATGCTCGCGAATATGGAATTGGTGCACAGATTCTAAGAGACATAGGAGTTCGAACGATGAGGCTGATGACAAACAATCCAGCCAAGTTCAGTGGTCTAAAGGGATATGGTTTGGCTGTGATTGGTAGGGTTCCGGTGTTAACACCAATTACAGAGGAAAACAAGAAGTACTTGGAAACAAAAAGAACTAAGATGGGGCATGTTTATGGTTCTGATATCCAAGGATCTTCAGCCGGTTTCATCAAACCAAATGTATATAACAAGGACATAGAAAACAAACCTGAAAGCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006766 GO:0006767 GO:0006771 GO:0006807 GO:0008150 GO:0008152 GO:0008686 GO:0009058 GO:0009110 GO:0009231 GO:0009987 GO:0016829 GO:0016830 GO:0017144 GO:0018130 GO:0034641 GO:0042364 GO:0042726 GO:0042727 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko00790 ko01100 ko01110 map00740 map00790 map01100 map01110

Pfam Domains

Protein Families

DHBP_synthase GTP_cyclohydro2

Protein Analysis

701

Amino Acids

76.71

Weight (kDa)

6.34

Isoelectric Point (pI)

43.87

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHBP_synthase	PF00926	279 - 473	6e-70	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	485 - 649	7.5e-71	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0011803)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59750 AT5G59750 AT5G59750
fragaria_vesca	FvH4_3g34860 FvH4_3g34860
malus_domestica	MD03G1100600.v1.1
prunus_persica	Prupe.6G085600_v2.0.a1 Prupe.6G085600_v2.0.a1
pyrus_communis	pycom03g08270
rosa_chinensis	RchiOBHm_Chr5g0062711
rosa_laevigata	RLG00000035574
rosa_multiflora	Rmu_ssc0000472.1_g000019
rosa_roxburghii	Rroxscaffold_1G00017910
rosa_rugosa	Rorug05G0351100
rosa_samantha	Rh5AG412100 Rh5BG426100 Rh5CG450200 Rh5DG440100
rosa_wichuraiana	Rw5G038740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	94
AccB1I	GGYRCC	1 cut(s)	156
AccB7I	CCANNNNNTGG	1 cut(s)	734
AccBSI	CCGCTC	1 cut(s)	1506
AccI	GTMKAC	1 cut(s)	346
AccII	CGCG	1 cut(s)	1821
AccIII	TCCGGA	1 cut(s)	1126
AciI	CCGC	3 cut(s)	1219, 1504, 1624
AclWI	GGATC	6 cut(s)	401, 794, 979, 986, 1014, 2044
AcoI	YGGCCR	2 cut(s)	380, 1195
AcsI	RAATTY	3 cut(s)	100, 253, 493
AcuI	CTGAAG	1 cut(s)	2025
AcyI	GRCGYC	2 cut(s)	95, 1343
AfaI	GTAC	3 cut(s)	1217, 1418, 1982
AfeI	AGCGCT	1 cut(s)	843
AfiI	CCNNNNNNNGG	4 cut(s)	734, 818, 919, 1228
AflII	CTTAAG	1 cut(s)	1288
AflIII	ACRYGT	1 cut(s)	1528
AhlI	ACTAGT	1 cut(s)	37
AjiI	CACGTC	1 cut(s)	164
AjnI	CCWGG	2 cut(s)	1192, 1782
AjuI	GAANNNNNNNTTGG	2 cut(s)	1815, 1847
Alw21I	GWGCWC	2 cut(s)	8, 1840
Alw44I	GTGCAC	1 cut(s)	1836
AlwI	GGATC	6 cut(s)	401, 794, 979, 986, 1014, 2044
AlwNI	CAGNNNCTG	1 cut(s)	1301
Ama87I	CYCGRG	1 cut(s)	553
Aor13HI	TCCGGA	1 cut(s)	1126
Aor51HI	AGCGCT	1 cut(s)	843
AoxI	GGCC	5 cut(s)	380, 1190, 1195, 1292, 1768
ApaLI	GTGCAC	1 cut(s)	1836
ApeKI	GCWGC	5 cut(s)	239, 422, 575, 923, 1660
ApoI	RAATTY	3 cut(s)	100, 253, 493
Asp700I	GAANNNNTTC	2 cut(s)	477, 649
AspLEI	GCGC	1 cut(s)	844
AspS9I	GGNCC	1 cut(s)	1375
AsuHPI	GGTGA	7 cut(s)	208, 280, 356, 515, 809, 1029, 1616
AsuII	TTCGAA	1 cut(s)	1864
AvaI	CYCGRG	1 cut(s)	553
AvaII	GGWCC	1 cut(s)	1375
BaeGI	GKGCMC	1 cut(s)	1840
BaeI	ACNNNNGTAYC	2 cut(s)	1101, 1134
BalI	TGGCCA	2 cut(s)	382, 1197
BanI	GGYRCC	1 cut(s)	156
BanII	GRGCYC	1 cut(s)	8
BbsI	GAAGAC	2 cut(s)	1056, 1062
Bbv12I	GWGCWC	2 cut(s)	8, 1840
BbvI	GCAGC	5 cut(s)	251, 409, 587, 935, 1672
BccI	CCATC	3 cut(s)	820, 1325, 1999
BceAI	ACGGC	2 cut(s)	1298, 1673
BciT130I	CCWGG	2 cut(s)	1194, 1784
BcuI	ACTAGT	1 cut(s)	37
BfaI	CTAG	6 cut(s)	38, 462, 630, 942, 1514, 1574
BfmI	CTRYAG	1 cut(s)	987
BfoI	RGCGCY	1 cut(s)	845
BfrI	CTTAAG	1 cut(s)	1288
BglII	AGATCT	1 cut(s)	10
BisI	GCNGC	5 cut(s)	240, 423, 576, 924, 1661
BlsI	GCNGC	5 cut(s)	241, 424, 577, 925, 1662
BmcAI	AGTACT	1 cut(s)	1982
Bme1390I	CCNGG	2 cut(s)	1194, 1784
Bme18I	GGWCC	1 cut(s)	1375
BmeT110I	CYCGRG	1 cut(s)	553
BmgBI	CACGTC	1 cut(s)	164
BmgT120I	GGNCC	1 cut(s)	1375
BmiI	GGNNCC	3 cut(s)	158, 1687, 1944
BmrFI	CCNGG	2 cut(s)	1194, 1784
BmsI	GCATC	7 cut(s)	571, 736, 778, 935, 1081, 1312, 1804
BpiI	GAAGAC	2 cut(s)	1056, 1062
BplI	GAGNNNNNCTC	2 cut(s)	809, 841
BpmI	CTGGAG	2 cut(s)	657, 1134
Bpu14I	TTCGAA	1 cut(s)	1864
BpuEI	CTTGAG	1 cut(s)	1196
Bsa29I	ATCGAT	3 cut(s)	334, 565, 1812
BsaBI	GATNNNNATC	1 cut(s)	330
BsaHI	GRCGYC	2 cut(s)	95, 1343
BsaI	GGTCTC	3 cut(s)	414, 758, 1037
BsaJI	CCNNGG	6 cut(s)	377, 792, 859, 1222, 1765, 2032
BsaWI	WCCGGW	2 cut(s)	1126, 1945
BsaXI	ACNNNNNCTCC	6 cut(s)	1231, 1261, 1554, 1584, 1677, 1707
Bsc4I	CCNNNNNNNGG	4 cut(s)	734, 818, 919, 1228
Bse118I	RCCGGY	2 cut(s)	179, 2045
Bse1I	ACTGG	5 cut(s)	410, 640, 1117, 1295, 1642
Bse3DI	GCAATG	2 cut(s)	90, 1662
Bse8I	GATNNNNATC	1 cut(s)	330
BseAI	TCCGGA	1 cut(s)	1126
BseBI	CCWGG	2 cut(s)	1194, 1784
BseCI	ATCGAT	3 cut(s)	334, 565, 1812
BseDI	CCNNGG	6 cut(s)	377, 792, 859, 1222, 1765, 2032
BseGI	GGATG	3 cut(s)	540, 751, 1096
BseJI	GATNNNNATC	1 cut(s)	330
BseLI	CCNNNNNNNGG	4 cut(s)	734, 818, 919, 1228
BseMI	GCAATG	2 cut(s)	90, 1662
BseMII	CTCAG	4 cut(s)	21, 596, 741, 1245
BseNI	ACTGG	5 cut(s)	410, 640, 1117, 1295, 1642
BseRI	GAGGAG	4 cut(s)	196, 570, 1016, 1698
BseSI	GKGCMC	1 cut(s)	1840
BseXI	GCAGC	5 cut(s)	251, 409, 587, 935, 1672
Bsh1236I	CGCG	1 cut(s)	1821
BshFI	GGCC	5 cut(s)	382, 1192, 1197, 1294, 1770
BshNI	GGYRCC	1 cut(s)	156
BshVI	ATCGAT	3 cut(s)	334, 565, 1812
BsiHKAI	GWGCWC	2 cut(s)	8, 1840
BsiHKCI	CYCGRG	1 cut(s)	553
BsiSI	CCGG	5 cut(s)	180, 1127, 1175, 1946, 2046
BslFI	GGGAC	1 cut(s)	751
BslI	CCNNNNNNNGG	4 cut(s)	734, 818, 919, 1228
BsmBI	CGTCTC	3 cut(s)	179, 422, 988
BsmFI	GGGAC	1 cut(s)	751
BsmI	GAATGC	2 cut(s)	953, 1383
BsnI	GGCC	5 cut(s)	382, 1192, 1197, 1294, 1770
Bso31I	GGTCTC	3 cut(s)	414, 758, 1037
BsoBI	CYCGRG	1 cut(s)	553
Bsp119I	TTCGAA	1 cut(s)	1864
Bsp1286I	GDGCHC	2 cut(s)	8, 1840
Bsp13I	TCCGGA	1 cut(s)	1126
Bsp143I	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
Bsp19I	CCATGG	2 cut(s)	377, 1222
Bsp68I	TCGCGA	1 cut(s)	1821
BspACI	CCGC	3 cut(s)	1219, 1504, 1624
BspANI	GGCC	5 cut(s)	382, 1192, 1197, 1294, 1770
BspCNI	CTCAG	4 cut(s)	20, 595, 742, 1246
BspDI	ATCGAT	3 cut(s)	334, 565, 1812
BspEI	TCCGGA	1 cut(s)	1126
BspFNI	CGCG	1 cut(s)	1821
BspLI	GGNNCC	3 cut(s)	158, 1687, 1944
BspPI	GGATC	6 cut(s)	401, 794, 979, 986, 1014, 2044
BspQI	GCTCTTC	1 cut(s)	451
BspT104I	TTCGAA	1 cut(s)	1864
BspT107I	GGYRCC	1 cut(s)	156
BspTI	CTTAAG	1 cut(s)	1288
BspTNI	GGTCTC	3 cut(s)	414, 758, 1037
BsrBI	CCGCTC	1 cut(s)	1506
BsrDI	GCAATG	2 cut(s)	90, 1662
BsrFI	RCCGGY	2 cut(s)	179, 2045
BsrI	ACTGG	5 cut(s)	410, 640, 1117, 1295, 1642
BssAI	RCCGGY	2 cut(s)	179, 2045
BssECI	CCNNGG	6 cut(s)	377, 792, 859, 1222, 1765, 2032
BssMI	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
BssNI	GRCGYC	2 cut(s)	95, 1343
BssT1I	CCWWGG	5 cut(s)	377, 859, 1222, 1765, 2032
Bst2UI	CCWGG	2 cut(s)	1194, 1784
Bst4CI	ACNGT	5 cut(s)	292, 1087, 1144, 1368, 1780
Bst6I	CTCTTC	5 cut(s)	216, 451, 514, 1066, 1711
BstACI	GRCGYC	2 cut(s)	95, 1343
BstAFI	CTTAAG	1 cut(s)	1288
BstAPI	GCANNNNNTGC	1 cut(s)	932
BstBI	TTCGAA	1 cut(s)	1864
BstC8I	GCNNGC	3 cut(s)	840, 1285, 1819
BstDEI	CTNAG	6 cut(s)	7, 582, 750, 1254, 1847, 2001
BstDSI	CCRYGG	2 cut(s)	377, 1222
BstF5I	GGATG	3 cut(s)	540, 751, 1096
BstFNI	CGCG	1 cut(s)	1821
BstH2I	RGCGCY	1 cut(s)	845
BstHHI	GCGC	1 cut(s)	844
BstKTI	GATC	9 cut(s)	13, 396, 802, 974, 981, 1009, 1270, 1408, 2039
BstMBI	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
BstMWI	GCNNNNNNNGC	3 cut(s)	458, 722, 932
BstNI	CCWGG	2 cut(s)	1194, 1784
BstNSI	RCATGY	2 cut(s)	1532, 2015
BstSCI	CCNGG	2 cut(s)	1192, 1782
BstSFI	CTRYAG	1 cut(s)	987
BstSLI	GKGCMC	1 cut(s)	1840
BstUI	CGCG	1 cut(s)	1821
BstV1I	GCAGC	5 cut(s)	251, 409, 587, 935, 1672
BstV2I	GAAGAC	2 cut(s)	1056, 1062
BstX2I	RGATCY	4 cut(s)	10, 971, 1006, 2036
BstXI	CCANNNNNNTGG	2 cut(s)	390, 612
BstYI	RGATCY	4 cut(s)	10, 971, 1006, 2036
Bsu15I	ATCGAT	3 cut(s)	334, 565, 1812
BsuRI	GGCC	5 cut(s)	382, 1192, 1197, 1294, 1770
BsuTUI	ATCGAT	3 cut(s)	334, 565, 1812
BtgI	CCRYGG	2 cut(s)	377, 1222
BtrI	CACGTC	1 cut(s)	164
BtsCI	GGATG	3 cut(s)	540, 751, 1096
BtsIMutI	CAGTG	4 cut(s)	1092, 1251, 1302, 1909
BtuMI	TCGCGA	1 cut(s)	1821
Cac8I	GCNNGC	3 cut(s)	840, 1285, 1819
CaiI	CAGNNNCTG	1 cut(s)	1301
CfoI	GCGC	1 cut(s)	844
Cfr10I	RCCGGY	2 cut(s)	179, 2045
Cfr13I	GGNCC	1 cut(s)	1375
ClaI	ATCGAT	3 cut(s)	334, 565, 1812
CseI	GACGC	2 cut(s)	84, 1332
Csp6I	GTAC	3 cut(s)	1216, 1417, 1981
CspCI	CAANNNNNGTGG	4 cut(s)	1068, 1103, 1885, 1920
CviAII	CATG	9 cut(s)	299, 378, 919, 994, 1199, 1223, 1529, 1772, 2012
CviQI	GTAC	3 cut(s)	1216, 1417, 1981
DdeI	CTNAG	6 cut(s)	7, 582, 750, 1254, 1847, 2001
DpnI	GATC	9 cut(s)	12, 395, 801, 973, 980, 1008, 1269, 1407, 2038
DpnII	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
DraI	TTTAAA	1 cut(s)	1359
DrdI	GACNNNNNNGTC	1 cut(s)	94
DseDI	GACNNNNNNGTC	1 cut(s)	94
EaeI	YGGCCR	2 cut(s)	380, 1195
Eam1104I	CTCTTC	5 cut(s)	216, 451, 514, 1066, 1711
EarI	CTCTTC	5 cut(s)	216, 451, 514, 1066, 1711
Ecl136II	GAGCTC	1 cut(s)	6
Eco130I	CCWWGG	5 cut(s)	377, 859, 1222, 1765, 2032
Eco147I	AGGCCT	1 cut(s)	1192
Eco24I	GRGCYC	1 cut(s)	8
Eco31I	GGTCTC	3 cut(s)	414, 758, 1037
Eco32I	GATATC	1 cut(s)	2029
Eco47I	GGWCC	1 cut(s)	1375
Eco47III	AGCGCT	1 cut(s)	843
Eco53kI	GAGCTC	1 cut(s)	6
Eco57I	CTGAAG	1 cut(s)	2025
Eco88I	CYCGRG	1 cut(s)	553
EcoICRI	GAGCTC	1 cut(s)	6
EcoRI	GAATTC	1 cut(s)	100
EcoRII	CCWGG	2 cut(s)	1192, 1782
EcoRV	GATATC	1 cut(s)	2029
EcoT14I	CCWWGG	5 cut(s)	377, 859, 1222, 1765, 2032
EcoT38I	GRGCYC	1 cut(s)	8
ErhI	CCWWGG	5 cut(s)	377, 859, 1222, 1765, 2032
Esp3I	CGTCTC	3 cut(s)	179, 422, 988
FaeI	CATG	9 cut(s)	302, 381, 922, 997, 1202, 1226, 1532, 1775, 2015
FalI	AAGNNNNNCTT	4 cut(s)	897, 929, 1967, 1999
FaqI	GGGAC	1 cut(s)	751
FatI	CATG	9 cut(s)	298, 377, 918, 993, 1198, 1222, 1528, 1771, 2011
FblI	GTMKAC	1 cut(s)	346
Fnu4HI	GCNGC	5 cut(s)	240, 423, 576, 924, 1661
FokI	GGATG	3 cut(s)	527, 758, 1103
FriOI	GRGCYC	1 cut(s)	8
Fsp4HI	GCNGC	5 cut(s)	240, 423, 576, 924, 1661
FspBI	CTAG	6 cut(s)	38, 462, 630, 942, 1514, 1574
GlaI	GCGC	1 cut(s)	843
GluI	GCNGC	5 cut(s)	240, 423, 576, 924, 1661
GsuI	CTGGAG	2 cut(s)	657, 1134
HaeII	RGCGCY	1 cut(s)	845
HaeIII	GGCC	5 cut(s)	382, 1192, 1197, 1294, 1770
HapII	CCGG	5 cut(s)	180, 1127, 1175, 1946, 2046
HgaI	GACGC	2 cut(s)	84, 1332
HhaI	GCGC	1 cut(s)	844
Hin1I	GRCGYC	2 cut(s)	95, 1343
Hin1II	CATG	9 cut(s)	302, 381, 922, 997, 1202, 1226, 1532, 1775, 2015
Hin6I	GCGC	1 cut(s)	842
HinP1I	GCGC	1 cut(s)	842
HincII	GTYRAC	3 cut(s)	310, 347, 1953
HindII	GTYRAC	3 cut(s)	310, 347, 1953
HindIII	AAGCTT	2 cut(s)	43, 1022
HpaI	GTTAAC	1 cut(s)	1953
HpaII	CCGG	5 cut(s)	180, 1127, 1175, 1946, 2046
HphI	GGTGA	7 cut(s)	208, 280, 356, 515, 809, 1029, 1616
Hpy166II	GTNNAC	5 cut(s)	310, 347, 1696, 1838, 1953
Hpy188III	TCNNGA	8 cut(s)	167, 630, 803, 1010, 1127, 1709, 1760, 1820
Hpy8I	GTNNAC	5 cut(s)	310, 347, 1696, 1838, 1953
Hpy99I	CGWCG	4 cut(s)	100, 130, 348, 351
HpyAV	CCTTC	7 cut(s)	187, 377, 899, 991, 1182, 1706, 1709
HpyCH4III	ACNGT	5 cut(s)	292, 1087, 1144, 1368, 1780
HpyCH4IV	ACGT	6 cut(s)	125, 163, 188, 387, 1354, 1462
HpyCH4V	TGCA	6 cut(s)	575, 935, 1094, 1325, 1660, 1838
HpyF10VI	GCNNNNNNNGC	3 cut(s)	458, 722, 932
HpyF3I	CTNAG	6 cut(s)	7, 582, 750, 1254, 1847, 2001
HpySE526I	ACGT	6 cut(s)	125, 163, 188, 387, 1354, 1462
Hsp92I	GRCGYC	2 cut(s)	95, 1343
Hsp92II	CATG	9 cut(s)	302, 381, 922, 997, 1202, 1226, 1532, 1775, 2015
HspAI	GCGC	1 cut(s)	842
Kpn2I	TCCGGA	1 cut(s)	1126
KspAI	GTTAAC	1 cut(s)	1953
Kzo9I	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
LguI	GCTCTTC	1 cut(s)	451
LmnI	GCTCC	4 cut(s)	80, 583, 1129, 1685
Lsp1109I	GCAGC	5 cut(s)	251, 409, 587, 935, 1672
LweI	GCATC	7 cut(s)	571, 736, 778, 935, 1081, 1312, 1804
MaeI	CTAG	6 cut(s)	38, 462, 630, 942, 1514, 1574
MaeII	ACGT	6 cut(s)	125, 163, 188, 387, 1354, 1462
MaeIII	GTNAC	7 cut(s)	196, 286, 1035, 1247, 1604, 1706, 1733
MalI	GATC	9 cut(s)	12, 395, 801, 973, 980, 1008, 1269, 1407, 2038
MbiI	CCGCTC	1 cut(s)	1506
MboI	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
MfeI	CAATTG	1 cut(s)	1389
MflI	RGATCY	4 cut(s)	10, 971, 1006, 2036
MhlI	GDGCHC	2 cut(s)	8, 1840
MlsI	TGGCCA	2 cut(s)	382, 1197
MluNI	TGGCCA	2 cut(s)	382, 1197
MlyI	GAGTC	7 cut(s)	314, 442, 492, 500, 719, 1052, 1175
MmeI	TCCRAC	1 cut(s)	1626
Mox20I	TGGCCA	2 cut(s)	382, 1197
MroI	TCCGGA	1 cut(s)	1126
MroXI	GAANNNNTTC	2 cut(s)	477, 649
MscI	TGGCCA	2 cut(s)	382, 1197
MseI	TTAA	4 cut(s)	963, 1289, 1358, 1952
MslI	CAYNNNNRTG	1 cut(s)	2016
Msp20I	TGGCCA	2 cut(s)	382, 1197
MspA1I	CMGCKG	1 cut(s)	242
MspCI	CTTAAG	1 cut(s)	1288
MspI	CCGG	5 cut(s)	180, 1127, 1175, 1946, 2046
MspR9I	CCNGG	2 cut(s)	1194, 1784
MunI	CAATTG	1 cut(s)	1389
Mva1269I	GAATGC	2 cut(s)	953, 1383
MvaI	CCWGG	2 cut(s)	1194, 1784
MvnI	CGCG	1 cut(s)	1821
MwoI	GCNNNNNNNGC	3 cut(s)	458, 722, 932
NcoI	CCATGG	2 cut(s)	377, 1222
NdeII	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
NlaIII	CATG	9 cut(s)	302, 381, 922, 997, 1202, 1226, 1532, 1775, 2015
NlaIV	GGNNCC	3 cut(s)	158, 1687, 1944
NmeAIII	GCCGAG	3 cut(s)	133, 451, 817
NmuCI	GTSAC	7 cut(s)	196, 286, 1035, 1247, 1604, 1706, 1733
NruI	TCGCGA	1 cut(s)	1821
NspI	RCATGY	2 cut(s)	1532, 2015
NspV	TTCGAA	1 cut(s)	1864
PaeR7I	CTCGAG	1 cut(s)	553
PceI	AGGCCT	1 cut(s)	1192
PciI	ACATGT	1 cut(s)	1528
PciSI	GCTCTTC	1 cut(s)	451
PcsI	WCGNNNNNNNCGW	4 cut(s)	95, 160, 340, 1818
PctI	GAATGC	2 cut(s)	953, 1383
PdmI	GAANNNNTTC	2 cut(s)	477, 649
PfeI	GAWTC	5 cut(s)	326, 361, 601, 806, 1843
PflFI	GACNNNGTC	1 cut(s)	1778
PflMI	CCANNNNNTGG	1 cut(s)	734
PkrI	GCNGC	5 cut(s)	241, 424, 577, 925, 1662
PleI	GAGTC	7 cut(s)	314, 441, 491, 500, 718, 1052, 1174
PpsI	GAGTC	7 cut(s)	314, 441, 491, 500, 718, 1052, 1174
PscI	ACATGT	1 cut(s)	1528
Psp124BI	GAGCTC	1 cut(s)	8
Psp6I	CCWGG	2 cut(s)	1192, 1782
PspGI	CCWGG	2 cut(s)	1192, 1782
PspN4I	GGNNCC	3 cut(s)	158, 1687, 1944
PspPI	GGNCC	1 cut(s)	1375
PspXI	VCTCGAGB	1 cut(s)	553
PstNI	CAGNNNCTG	1 cut(s)	1301
PsuI	RGATCY	4 cut(s)	10, 971, 1006, 2036
PsyI	GACNNNGTC	1 cut(s)	1778
PvuII	CAGCTG	1 cut(s)	242
RruI	TCGCGA	1 cut(s)	1821
RsaI	GTAC	3 cut(s)	1217, 1418, 1982
RsaNI	GTAC	3 cut(s)	1216, 1417, 1981
RseI	CAYNNNNRTG	1 cut(s)	2016
SacI	GAGCTC	1 cut(s)	8
SalI	GTCGAC	1 cut(s)	345
SapI	GCTCTTC	1 cut(s)	451
SaqAI	TTAA	4 cut(s)	963, 1289, 1358, 1952
SatI	GCNGC	5 cut(s)	240, 423, 576, 924, 1661
Sau3AI	GATC	9 cut(s)	10, 393, 799, 971, 978, 1006, 1267, 1405, 2036
Sau96I	GGNCC	1 cut(s)	1375
ScaI	AGTACT	1 cut(s)	1982
SchI	GAGTC	7 cut(s)	314, 442, 492, 500, 719, 1052, 1175
ScrFI	CCNGG	2 cut(s)	1194, 1784
SduI	GDGCHC	2 cut(s)	8, 1840
SfaNI	GCATC	7 cut(s)	571, 736, 778, 935, 1081, 1312, 1804
SfcI	CTRYAG	1 cut(s)	987
Sfr274I	CTCGAG	1 cut(s)	553
SfuI	TTCGAA	1 cut(s)	1864
SgrAI	CRCCGGYG	1 cut(s)	179
SgrDI	CGTCGACG	1 cut(s)	345
SinI	GGWCC	1 cut(s)	1375
SlaI	CTCGAG	1 cut(s)	553
SmiMI	CAYNNNNRTG	1 cut(s)	2016
SmlI	CTYRAG	3 cut(s)	553, 1211, 1288
SmoI	CTYRAG	3 cut(s)	553, 1211, 1288
SpeI	ACTAGT	1 cut(s)	37
SseBI	AGGCCT	1 cut(s)	1192
SsiI	CCGC	3 cut(s)	1219, 1504, 1624
SspMI	CTAG	6 cut(s)	38, 462, 630, 942, 1514, 1574
SstI	GAGCTC	1 cut(s)	8
StuI	AGGCCT	1 cut(s)	1192
StyD4I	CCNGG	2 cut(s)	1192, 1782
StyI	CCWWGG	5 cut(s)	377, 859, 1222, 1765, 2032
TaaI	ACNGT	5 cut(s)	292, 1087, 1144, 1368, 1780
TaiI	ACGT	6 cut(s)	128, 166, 191, 390, 1357, 1465
TaqII	GACCGA	1 cut(s)	1061
TatI	WGTACW	1 cut(s)	1980
TfiI	GAWTC	5 cut(s)	326, 361, 601, 806, 1843
Tru1I	TTAA	4 cut(s)	963, 1289, 1358, 1952
Tru9I	TTAA	4 cut(s)	963, 1289, 1358, 1952
TscAI	CASTG	4 cut(s)	1092, 1258, 1302, 1909
TseFI	GTSAC	7 cut(s)	196, 286, 1035, 1247, 1604, 1706, 1733
TseI	GCWGC	5 cut(s)	239, 422, 575, 923, 1660
Tsp45I	GTSAC	7 cut(s)	196, 286, 1035, 1247, 1604, 1706, 1733
TspDTI	ATGAA	7 cut(s)	287, 708, 903, 1010, 1146, 1574, 2041
TspGWI	ACGGA	4 cut(s)	295, 332, 984, 1533
TspRI	CASTG	4 cut(s)	1092, 1258, 1302, 1909
Tth111I	GACNNNGTC	1 cut(s)	1778
Van91I	CCANNNNNTGG	1 cut(s)	734
Vha464I	CTTAAG	1 cut(s)	1288
VneI	GTGCAC	1 cut(s)	1836
VpaK11BI	GGWCC	1 cut(s)	1375
XapI	RAATTY	3 cut(s)	100, 253, 493
XbaI	TCTAGA	1 cut(s)	629
XceI	RCATGY	2 cut(s)	1532, 2015
XhoI	CTCGAG	1 cut(s)	553
XmiI	GTMKAC	1 cut(s)	346
XmnI	GAANNNNTTC	2 cut(s)	477, 649
XspI	CTAG	6 cut(s)	38, 462, 630, 942, 1514, 1574
ZrmI	AGTACT	1 cut(s)	1982

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.