Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0011803

Physical Location & Seq

Genomic Coordinates Reverse (-)

8591060 .. 8594116

Gene Structure 3057 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1100600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1644 bp

ATGGACTCTGCTCTAGCTCCTCACCCCCTCTTCCCTCATATCCTCTCTAGCTCGAGCTTGCATCGATCTCTCGCAGTTCGTCAATGTCTGTTCCGGCAGAGGTGGTTAAATCCGACTTGTTGGGCTATTGGGGTGTCTGGAAGTAGTGTTGGAGCAGCTGGGAATAACCTCTTTGGTGATGCTAATTATTTGAAGGGGAGTGAAAATGGTTCTCTGTTGAGTGGGTTAGATGAGTCAGCTTCGGCAGGACCATTTGGGACGTTGGATGCGGAGATCACACCGGAGACCGTTGATTTCTTTGTTAGTGATGCTGATGGTGACCCTGATTGCCCATCTGACGGCTACTCTTCCATAGAGCAGGCACTTAACACATTACGCCAAGGAAAGTTTGTGATTGTTGTAGATGATGAAAATGGGGAAGTTGAAGGAAACCTAGTCATGGCCGCGTCGTTTGCGAACCCGAAAAACGTAGCGTTCATGATCAAGCAGGGGTCTGGGATTGTCTCAGTAGGCATGATGGAGGAGGATCTTGAAAGATTGGAGCTTCCTCTCATGTCACCGGAGACTGAAGATGAAGACTCTTCTGCTCCCACATTCACAATCACAGTGGATGCAAAAGTTGGAACATCAACCGGAGTCTCAGCTGCAGACAGGGCAAAAACTGTGGCTGCTCTTTCATCCAATGAGTCTAAACCGGAGGACTTCAGAAGGCCTGGCCATGTGTTTCCCCTCAAGTACCGAAATGGCGGGGTTCTTAGGAGAGCGGGTCACACTGAGGCTTCGGTTGATCTCGTTGTTCTGGCCGGCTTAAGGCCGGTGTCTGTTCTTTCAGCAGTAGTTGACGCCGATGACGGCTCTATGGCCTCTTTGTCCAATTTGAGGACATTGTCTCTGGAGCATTCCATTCCAATTGTCTCCATTACTGATTTGATCAGGTACAGGAGAAAGAGGGAAAAATTAGTTGAAAGAAAAGCCGTTTCGCGTCTACCTACGAAATGGGGTCTATTTCAAGCCTATTGCTATCGCTCGAAGCTAGACGGAACAGAACATGTTGCAGTTGTAAAGGGAAGCATTGGAAATGGACAAGATGTGCTAGTAAGAGTGCATTCGGAGTGTTTGACAGGAGATATTTTCGGATCAGCGCGGTGCGACTGTGGAAACCAGTTGGATGTGGCAATGCAGCTAATCGAACAAGCCGGTCGAGGAGTCGTTGTGTACCTCAGAGGTCATGAAGGAAGAGGCATTGGTCTAGGCCACAAACTTCAAGCCTATAATCTGCAGGATCAAGGCCATGACACCGTCCAGGCCAACATAGAACTCGGACTAGCTGTTGATTCTCGTGAATATGGGATTGGTGCCCAGATATTGAGAGACATAGGGGTCCGAACGATGAGGCTAATGACGAACAACCCGGCAAAGTTCACCGGTCTAAAGGGATACGGGTTGGCCGTGGTCGGGAGGGTTCCGGTTTTGACACCAATCACAGAGGAAAACAAGACCTACTTGGAAACAAAAAGAACAAAGATGGGACATGTTTACGGTTCTGATATTCAAGGACTTTCAGATGGATTGTTCATCAACCCTAATGTAAATGACCAAGGTCTGCCGGAATCAGGAAATACCGAAGATTCAAATTATACGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006766 GO:0006767 GO:0006771 GO:0006807 GO:0008150 GO:0008152 GO:0008686 GO:0009058 GO:0009110 GO:0009231 GO:0009987 GO:0016829 GO:0016830 GO:0017144 GO:0018130 GO:0034641 GO:0042364 GO:0042726 GO:0042727 GO:0044237 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576

KEGG Pathways

Metabolic & Signaling

ko00740 ko00790 ko01100 ko01110 map00740 map00790 map01100 map01110

Pfam Domains

Protein Families

DHBP_synthase GTP_cyclohydro2

Protein Analysis

548

Amino Acids

58.77

Weight (kDa)

5.29

Isoelectric Point (pI)

37.94

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHBP_synthase	PF00926	119 - 313	1.6e-71	3,4-dihydroxy-2-butanone 4-phosphate synthase
GTP_cyclohydro2	PF00925	323 - 489	1.9e-72	GTP cyclohydrolase II

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011803)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G59750 AT5G59750 AT5G59750
fragaria_vesca	FvH4_3g34860 FvH4_3g34860
malus_domestica	MD03G1100600.v1.1
prunus_persica	Prupe.6G085600_v2.0.a1 Prupe.6G085600_v2.0.a1
pyrus_communis	pycom03g08270
rosa_chinensis	RchiOBHm_Chr5g0062711
rosa_laevigata	RLG00000035574
rosa_multiflora	Rmu_ssc0000472.1_g000019
rosa_roxburghii	Rroxscaffold_1G00017910
rosa_rugosa	Rorug05G0351100
rosa_samantha	Rh5AG412100 Rh5BG426100 Rh5CG450200 Rh5DG440100
rosa_wichuraiana	Rw5G038740

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1379
AccB1I	GGYRCC	1 cut(s)	1355
AccBSI	CCGCTC	1 cut(s)	764
AccI	GTMKAC	1 cut(s)	985
AccII	CGCG	3 cut(s)	446, 982, 1144
AciI	CCGC	5 cut(s)	269, 444, 747, 764, 1144
AclWI	GGATC	3 cut(s)	534, 1144, 1290
AcoI	YGGCCR	4 cut(s)	441, 715, 801, 1446
AcuI	CTGAAG	2 cut(s)	588, 688
AcyI	GRCGYC	1 cut(s)	843
AfaI	GTAC	3 cut(s)	737, 938, 1217
AfiI	CCNNNNNNNGG	6 cut(s)	338, 439, 810, 879, 1455, 1613
AflII	CTTAAG	1 cut(s)	808
AflIII	ACRYGT	2 cut(s)	1048, 1531
AgeI	ACCGGT	1 cut(s)	1424
AgsI	TTSAA	8 cut(s)	193, 425, 533, 965, 1010, 1265, 1553, 1632
AjnI	CCWGG	2 cut(s)	712, 1302
AjuI	GAANNNNNNNTTGG	2 cut(s)	1335, 1367
Alw26I	GTCTC	7 cut(s)	278, 508, 557, 643, 894, 919, 1365
AlwI	GGATC	3 cut(s)	534, 1144, 1290
Ama87I	CYCGRG	1 cut(s)	52
ApeKI	GCWGC	4 cut(s)	155, 644, 668, 1180
AsiGI	ACCGGT	1 cut(s)	1424
AspLEI	GCGC	1 cut(s)	1144
AspS9I	GGNCC	2 cut(s)	248, 1381
AsuC2I	CCSGG	1 cut(s)	1412
AsuHPI	GGTGA	5 cut(s)	14, 188, 329, 549, 1414
AvaI	CYCGRG	1 cut(s)	52
AvaII	GGWCC	2 cut(s)	248, 1381
BaeGI	GKGCMC	1 cut(s)	1360
BalI	TGGCCA	1 cut(s)	717
BanI	GGYRCC	1 cut(s)	1355
BauI	CACGAG	1 cut(s)	1338
BbsI	GAAGAC	1 cut(s)	582
BbvI	GCAGC	4 cut(s)	167, 631, 655, 1192
BccI	CCATC	5 cut(s)	308, 340, 511, 1519, 1559
BceAI	ACGGC	4 cut(s)	355, 868, 959, 1433
BciT130I	CCWGG	2 cut(s)	714, 1304
BciVI	GTATCC	1 cut(s)	1430
BclI	TGATCA	2 cut(s)	480, 930
BcnI	CCSGG	1 cut(s)	1412
BcoDI	GTCTC	7 cut(s)	278, 508, 557, 643, 894, 919, 1365
BfaI	CTAG	7 cut(s)	14, 48, 434, 1034, 1094, 1250, 1325
BfmI	CTRYAG	2 cut(s)	645, 1277
BfrI	CTTAAG	1 cut(s)	808
BfuI	GTATCC	1 cut(s)	1430
BisI	GCNGC	5 cut(s)	156, 444, 645, 669, 1181
BlsI	GCNGC	5 cut(s)	157, 445, 646, 670, 1182
Bme1390I	CCNGG	3 cut(s)	714, 1304, 1412
Bme18I	GGWCC	2 cut(s)	248, 1381
BmeT110I	CYCGRG	1 cut(s)	52
BmgT120I	GGNCC	2 cut(s)	248, 1381
BmiI	GGNNCC	3 cut(s)	1357, 1382, 1464
BmrFI	CCNGG	3 cut(s)	714, 1304, 1412
BmsI	GCATC	5 cut(s)	70, 169, 256, 298, 601
BoxI	GACNNNNGTC	1 cut(s)	1599
BpiI	GAAGAC	1 cut(s)	582
BpmI	CTGGAG	1 cut(s)	914
BpuEI	CTTGAG	1 cut(s)	716
BpuMI	CCSGG	1 cut(s)	1412
Bsa29I	ATCGAT	1 cut(s)	64
BsaAI	YACGTR	1 cut(s)	1641
BsaHI	GRCGYC	1 cut(s)	843
BsaI	GGTCTC	1 cut(s)	278
BsaJI	CCNNGG	3 cut(s)	379, 1449, 1597
BsaWI	WCCGGW	6 cut(s)	280, 559, 632, 694, 1424, 1465
BsaXI	ACNNNNNCTCC	4 cut(s)	751, 781, 1197, 1227
Bsc4I	CCNNNNNNNGG	6 cut(s)	338, 439, 810, 879, 1455, 1613
Bse118I	RCCGGY	4 cut(s)	803, 814, 1196, 1424
Bse1I	ACTGG	1 cut(s)	1162
Bse3DI	GCAATG	1 cut(s)	1182
BseBI	CCWGG	2 cut(s)	714, 1304
BseCI	ATCGAT	1 cut(s)	64
BseDI	CCNNGG	3 cut(s)	379, 1449, 1597
BseGI	GGATG	4 cut(s)	271, 616, 677, 1174
BseLI	CCNNNNNNNGG	6 cut(s)	338, 439, 810, 879, 1455, 1613
BseMI	GCAATG	1 cut(s)	1182
BseMII	CTCAG	4 cut(s)	519, 654, 765, 1234
BseNI	ACTGG	1 cut(s)	1162
BseRI	GAGGAG	3 cut(s)	9, 536, 1218
BseSI	GKGCMC	1 cut(s)	1360
BseXI	GCAGC	4 cut(s)	167, 631, 655, 1192
BseYI	CCCAGC	1 cut(s)	158
Bsh1236I	CGCG	3 cut(s)	446, 982, 1144
Bsh1285I	CGRYCG	1 cut(s)	1201
BshNI	GGYRCC	1 cut(s)	1355
BshTI	ACCGGT	1 cut(s)	1424
BshVI	ATCGAT	1 cut(s)	64
BsiEI	CGRYCG	1 cut(s)	1201
BsiHKCI	CYCGRG	1 cut(s)	52
BslFI	GGGAC	2 cut(s)	271, 1542
BslI	CCNNNNNNNGG	6 cut(s)	338, 439, 810, 879, 1455, 1613
BsmAI	GTCTC	7 cut(s)	278, 508, 557, 643, 894, 919, 1365
BsmFI	GGGAC	2 cut(s)	271, 1542
BsmI	GAATGC	2 cut(s)	898, 1105
Bso31I	GGTCTC	1 cut(s)	278
BsoBI	CYCGRG	1 cut(s)	52
Bsp1286I	GDGCHC	1 cut(s)	1360
Bsp143I	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
BspACI	CCGC	5 cut(s)	269, 444, 747, 764, 1144
BspCNI	CTCAG	4 cut(s)	518, 653, 766, 1233
BspDI	ATCGAT	1 cut(s)	64
BspFNI	CGCG	3 cut(s)	446, 982, 1144
BspHI	TCATGA	2 cut(s)	477, 1228
BspLI	GGNNCC	3 cut(s)	1357, 1382, 1464
BspMAI	CTGCAG	2 cut(s)	649, 1281
BspPI	GGATC	3 cut(s)	534, 1144, 1290
BspT107I	GGYRCC	1 cut(s)	1355
BspTI	CTTAAG	1 cut(s)	808
BspTNI	GGTCTC	1 cut(s)	278
BsrBI	CCGCTC	1 cut(s)	764
BsrDI	GCAATG	1 cut(s)	1182
BsrFI	RCCGGY	4 cut(s)	803, 814, 1196, 1424
BsrI	ACTGG	1 cut(s)	1162
BssAI	RCCGGY	4 cut(s)	803, 814, 1196, 1424
BssECI	CCNNGG	3 cut(s)	379, 1449, 1597
BssMI	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
BssNI	GRCGYC	1 cut(s)	843
BssSI	CACGAG	1 cut(s)	1338
BssT1I	CCWWGG	2 cut(s)	379, 1597
Bst2BI	CACGAG	1 cut(s)	1338
Bst2UI	CCWGG	2 cut(s)	714, 1304
Bst4CI	ACNGT	6 cut(s)	289, 607, 664, 1154, 1300, 1541
Bst6I	CTCTTC	4 cut(s)	35, 352, 586, 1231
BstACI	GRCGYC	1 cut(s)	843
BstAFI	CTTAAG	1 cut(s)	808
BstBAI	YACGTR	1 cut(s)	1641
BstC8I	GCNNGC	3 cut(s)	59, 360, 805
BstDEI	CTNAG	5 cut(s)	505, 640, 755, 774, 1220
BstDSI	CCRYGG	1 cut(s)	1449
BstEII	GGTNACC	1 cut(s)	317
BstF5I	GGATG	4 cut(s)	271, 616, 677, 1174
BstFNI	CGCG	3 cut(s)	446, 982, 1144
BstHHI	GCGC	1 cut(s)	1144
BstKTI	GATC	8 cut(s)	68, 276, 483, 529, 790, 933, 1139, 1285
BstMAI	GTCTC	7 cut(s)	278, 508, 557, 643, 894, 919, 1365
BstMBI	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
BstMCI	CGRYCG	1 cut(s)	1201
BstMWI	GCNNNNNNNGC	2 cut(s)	452, 653
BstNI	CCWGG	2 cut(s)	714, 1304
BstNSI	RCATGY	2 cut(s)	1052, 1535
BstPAI	GACNNNNGTC	1 cut(s)	1599
BstPI	GGTNACC	1 cut(s)	317
BstSCI	CCNGG	3 cut(s)	712, 1302, 1410
BstSFI	CTRYAG	2 cut(s)	645, 1277
BstSLI	GKGCMC	1 cut(s)	1360
BstSNI	TACGTA	1 cut(s)	1641
BstUI	CGCG	3 cut(s)	446, 982, 1144
BstV1I	GCAGC	4 cut(s)	167, 631, 655, 1192
BstV2I	GAAGAC	1 cut(s)	582
BstX2I	RGATCY	1 cut(s)	526
BstYI	RGATCY	1 cut(s)	526
Bsu15I	ATCGAT	1 cut(s)	64
BsuI	GTATCC	1 cut(s)	1430
BsuTUI	ATCGAT	1 cut(s)	64
BtgI	CCRYGG	1 cut(s)	1449
BtsCI	GGATG	4 cut(s)	271, 616, 677, 1174
BtsIMutI	CAGTG	2 cut(s)	612, 771
Cac8I	GCNNGC	3 cut(s)	59, 360, 805
CciI	TCATGA	2 cut(s)	477, 1228
CfoI	GCGC	1 cut(s)	1144
Cfr10I	RCCGGY	4 cut(s)	803, 814, 1196, 1424
Cfr13I	GGNCC	2 cut(s)	248, 1381
ClaI	ATCGAT	1 cut(s)	64
CseI	GACGC	3 cut(s)	435, 851, 971
Csp6I	GTAC	3 cut(s)	736, 937, 1216
CspAI	ACCGGT	1 cut(s)	1424
CspCI	CAANNNNNGTGG	4 cut(s)	588, 623, 645, 680
CviAII	CATG	9 cut(s)	439, 478, 514, 553, 719, 1049, 1229, 1292, 1532
CviQI	GTAC	3 cut(s)	736, 937, 1216
DdeI	CTNAG	5 cut(s)	505, 640, 755, 774, 1220
DpnI	GATC	8 cut(s)	67, 275, 482, 528, 789, 932, 1138, 1284
DpnII	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
DrdI	GACNNNNNNGTC	1 cut(s)	1379
DseDI	GACNNNNNNGTC	1 cut(s)	1379
EaeI	YGGCCR	4 cut(s)	441, 715, 801, 1446
Eam1104I	CTCTTC	4 cut(s)	35, 352, 586, 1231
EarI	CTCTTC	4 cut(s)	35, 352, 586, 1231
Eco105I	TACGTA	1 cut(s)	1641
Eco130I	CCWWGG	2 cut(s)	379, 1597
Eco147I	AGGCCT	1 cut(s)	712
Eco31I	GGTCTC	1 cut(s)	278
Eco47I	GGWCC	2 cut(s)	248, 1381
Eco57I	CTGAAG	2 cut(s)	588, 688
Eco88I	CYCGRG	1 cut(s)	52
Eco91I	GGTNACC	1 cut(s)	317
EcoO65I	GGTNACC	1 cut(s)	317
EcoRII	CCWGG	2 cut(s)	712, 1302
EcoT14I	CCWWGG	2 cut(s)	379, 1597
ErhI	CCWWGG	2 cut(s)	379, 1597
FaeI	CATG	9 cut(s)	442, 481, 517, 556, 722, 1052, 1232, 1295, 1535
FalI	AAGNNNNNCTT	2 cut(s)	1487, 1519
FaqI	GGGAC	2 cut(s)	271, 1542
FatI	CATG	9 cut(s)	438, 477, 513, 552, 718, 1048, 1228, 1291, 1531
FauI	CCCGC	2 cut(s)	740, 757
FbaI	TGATCA	2 cut(s)	480, 930
FblI	GTMKAC	1 cut(s)	985
Fnu4HI	GCNGC	5 cut(s)	156, 444, 645, 669, 1181
FokI	GGATG	4 cut(s)	278, 623, 664, 1181
Fsp4HI	GCNGC	5 cut(s)	156, 444, 645, 669, 1181
FspBI	CTAG	7 cut(s)	14, 48, 434, 1034, 1094, 1250, 1325
GlaI	GCGC	1 cut(s)	1143
GluI	GCNGC	5 cut(s)	156, 444, 645, 669, 1181
GsaI	CCCAGC	1 cut(s)	162
GsuI	CTGGAG	1 cut(s)	914
HgaI	GACGC	3 cut(s)	435, 851, 971
HhaI	GCGC	1 cut(s)	1144
Hin1I	GRCGYC	1 cut(s)	843
Hin1II	CATG	9 cut(s)	442, 481, 517, 556, 722, 1052, 1232, 1295, 1535
Hin6I	GCGC	1 cut(s)	1142
HinP1I	GCGC	1 cut(s)	1142
HincII	GTYRAC	1 cut(s)	841
HindII	GTYRAC	1 cut(s)	841
HinfI	GANTC	9 cut(s)	5, 233, 578, 636, 686, 1206, 1334, 1610, 1628
HphI	GGTGA	5 cut(s)	14, 188, 329, 549, 1414
Hpy166II	GTNNAC	5 cut(s)	841, 986, 1216, 1422, 1537
Hpy188III	TCNNGA	8 cut(s)	138, 478, 530, 893, 1229, 1340, 1456, 1614
Hpy8I	GTNNAC	5 cut(s)	841, 986, 1216, 1422, 1537
Hpy99I	CGWCG	1 cut(s)	451
HpyAV	CCTTC	4 cut(s)	187, 419, 702, 1226
HpyCH4III	ACNGT	6 cut(s)	289, 607, 664, 1154, 1300, 1541
HpyCH4IV	ACGT	3 cut(s)	260, 468, 1640
HpyCH4V	TGCA	7 cut(s)	61, 614, 647, 1055, 1105, 1180, 1279
HpyF10VI	GCNNNNNNNGC	2 cut(s)	452, 653
HpyF3I	CTNAG	5 cut(s)	505, 640, 755, 774, 1220
HpySE526I	ACGT	3 cut(s)	260, 468, 1640
Hsp92I	GRCGYC	1 cut(s)	843
Hsp92II	CATG	9 cut(s)	442, 481, 517, 556, 722, 1052, 1232, 1295, 1535
HspAI	GCGC	1 cut(s)	1142
KroI	GCCGGC	1 cut(s)	803
KroNI	GCCGGC	1 cut(s)	805
Ksp22I	TGATCA	2 cut(s)	480, 930
Kzo9I	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
LmnI	GCTCC	5 cut(s)	22, 152, 541, 592, 895
Lsp1109I	GCAGC	4 cut(s)	167, 631, 655, 1192
LweI	GCATC	5 cut(s)	70, 169, 256, 298, 601
MaeI	CTAG	7 cut(s)	14, 48, 434, 1034, 1094, 1250, 1325
MaeII	ACGT	3 cut(s)	260, 468, 1640
MaeIII	GTNAC	3 cut(s)	317, 555, 767
MalI	GATC	8 cut(s)	67, 275, 482, 528, 789, 932, 1138, 1284
MbiI	CCGCTC	1 cut(s)	764
MboI	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
MboII	GAAGA	7 cut(s)	22, 339, 573, 581, 587, 1248, 1637
MfeI	CAATTG	1 cut(s)	909
MflI	RGATCY	1 cut(s)	526
MhlI	GDGCHC	1 cut(s)	1360
MlsI	TGGCCA	1 cut(s)	717
MluCI	AATT	5 cut(s)	184, 874, 909, 956, 1633
MluNI	TGGCCA	1 cut(s)	717
MlyI	GAGTC	5 cut(s)	242, 572, 645, 695, 1215
MmeI	TCCRAC	5 cut(s)	130, 137, 243, 601, 1146
Mox20I	TGGCCA	1 cut(s)	717
MroNI	GCCGGC	1 cut(s)	803
MscI	TGGCCA	1 cut(s)	717
MseI	TTAA	3 cut(s)	107, 366, 809
Msp20I	TGGCCA	1 cut(s)	717
MspA1I	CMGCKG	2 cut(s)	158, 644
MspCI	CTTAAG	1 cut(s)	808
MspR9I	CCNGG	3 cut(s)	714, 1304, 1412
MunI	CAATTG	1 cut(s)	909
Mva1269I	GAATGC	2 cut(s)	898, 1105
MvaI	CCWGG	2 cut(s)	714, 1304
MvnI	CGCG	3 cut(s)	446, 982, 1144
MwoI	GCNNNNNNNGC	2 cut(s)	452, 653
NaeI	GCCGGC	1 cut(s)	805
NciI	CCSGG	1 cut(s)	1412
NdeII	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
NgoMIV	GCCGGC	1 cut(s)	803
NlaIII	CATG	9 cut(s)	442, 481, 517, 556, 722, 1052, 1232, 1295, 1535
NlaIV	GGNNCC	3 cut(s)	1357, 1382, 1464
NmuCI	GTSAC	3 cut(s)	317, 555, 767
NspI	RCATGY	2 cut(s)	1052, 1535
PaeR7I	CTCGAG	1 cut(s)	52
PagI	TCATGA	2 cut(s)	477, 1228
PceI	AGGCCT	1 cut(s)	712
PciI	ACATGT	2 cut(s)	1048, 1531
PcsI	WCGNNNNNNNCGW	1 cut(s)	1446
PctI	GAATGC	2 cut(s)	898, 1105
PdiI	GCCGGC	1 cut(s)	805
PfeI	GAWTC	3 cut(s)	1334, 1610, 1628
PflFI	GACNNNGTC	2 cut(s)	886, 1298
PinAI	ACCGGT	1 cut(s)	1424
PkrI	GCNGC	5 cut(s)	157, 445, 646, 670, 1182
PleI	GAGTC	5 cut(s)	241, 572, 644, 694, 1214
PpsI	GAGTC	5 cut(s)	241, 572, 644, 694, 1214
Ppu21I	YACGTR	1 cut(s)	1641
PscI	ACATGT	2 cut(s)	1048, 1531
PshAI	GACNNNNGTC	1 cut(s)	1599
Psp6I	CCWGG	2 cut(s)	712, 1302
PspEI	GGTNACC	1 cut(s)	317
PspFI	CCCAGC	1 cut(s)	158
PspGI	CCWGG	2 cut(s)	712, 1302
PspN4I	GGNNCC	3 cut(s)	1357, 1382, 1464
PspPI	GGNCC	2 cut(s)	248, 1381
PspXI	VCTCGAGB	1 cut(s)	52
PstI	CTGCAG	2 cut(s)	649, 1281
PsuI	RGATCY	1 cut(s)	526
PsyI	GACNNNGTC	2 cut(s)	886, 1298
PvuII	CAGCTG	2 cut(s)	158, 644
RsaI	GTAC	3 cut(s)	737, 938, 1217
RsaNI	GTAC	3 cut(s)	736, 937, 1216
SaqAI	TTAA	3 cut(s)	107, 366, 809
SatI	GCNGC	5 cut(s)	156, 444, 645, 669, 1181
Sau3AI	GATC	8 cut(s)	65, 273, 480, 526, 787, 930, 1136, 1282
Sau96I	GGNCC	2 cut(s)	248, 1381
SchI	GAGTC	5 cut(s)	242, 572, 645, 695, 1215
ScrFI	CCNGG	3 cut(s)	714, 1304, 1412
SduI	GDGCHC	1 cut(s)	1360
SfaNI	GCATC	5 cut(s)	70, 169, 256, 298, 601
SfcI	CTRYAG	2 cut(s)	645, 1277
Sfr274I	CTCGAG	1 cut(s)	52
SinI	GGWCC	2 cut(s)	248, 1381
SlaI	CTCGAG	1 cut(s)	52
SmlI	CTYRAG	3 cut(s)	52, 731, 808
SmoI	CTYRAG	3 cut(s)	52, 731, 808
SnaBI	TACGTA	1 cut(s)	1641
Sse9I	AATT	5 cut(s)	184, 874, 909, 956, 1633
SseBI	AGGCCT	1 cut(s)	712
SsiI	CCGC	5 cut(s)	269, 444, 747, 764, 1144
SspMI	CTAG	7 cut(s)	14, 48, 434, 1034, 1094, 1250, 1325
StuI	AGGCCT	1 cut(s)	712
StyD4I	CCNGG	3 cut(s)	712, 1302, 1410
StyI	CCWWGG	2 cut(s)	379, 1597
TaaI	ACNGT	6 cut(s)	289, 607, 664, 1154, 1300, 1541
TaiI	ACGT	3 cut(s)	263, 471, 1643
TaqI	TCGA	5 cut(s)	53, 64, 1028, 1188, 1201
TasI	AATT	5 cut(s)	184, 874, 909, 956, 1633
TauI	GCSGC	1 cut(s)	446
TfiI	GAWTC	3 cut(s)	1334, 1610, 1628
Tru1I	TTAA	3 cut(s)	107, 366, 809
Tru9I	TTAA	3 cut(s)	107, 366, 809
TscAI	CASTG	2 cut(s)	612, 778
TseFI	GTSAC	3 cut(s)	317, 555, 767
TseI	GCWGC	4 cut(s)	155, 644, 668, 1180
Tsp45I	GTSAC	3 cut(s)	317, 555, 767
TspDTI	ATGAA	6 cut(s)	423, 466, 588, 666, 1245, 1564
TspGWI	ACGGA	1 cut(s)	1053
TspRI	CASTG	2 cut(s)	612, 778
Tth111I	GACNNNGTC	2 cut(s)	886, 1298
Vha464I	CTTAAG	1 cut(s)	808
VpaK11BI	GGWCC	2 cut(s)	248, 1381
XceI	RCATGY	2 cut(s)	1052, 1535
XhoI	CTCGAG	1 cut(s)	52
XmiI	GTMKAC	1 cut(s)	985
XspI	CTAG	7 cut(s)	14, 48, 434, 1034, 1094, 1250, 1325

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.