AT5G64040

Photosystem I reaction centre subunit N (PSAN or PSI-N)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0014548
Physical Location & Seq
Reverse (-)
25628521 .. 25629708
1188 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G64040.2

Sequence Viewer

Length: 516 bp
ATGGCGGCCATGAACTCGAGTGTTCTCACTTGCAGCTACGCAATTGCTGGCTCTGGCTCGGTAGAGCTTAACCAGAAAGTTGGTTTGGTGAATTCATCAGTTGGGTTTGGTCAGAAGAAACAGATGATTATGCCTGTGATCAAAGCTCAACGCGTTGTTGGTGATGATGTTGATGGATCTAATGGAAGACGATCAGCCATGGTTTTCTTAGCAGCTACACTCTTCTCCACTGCTGCTGTTTCTGCTTCTGCTAATGCTGGCGTCATTGACGAATACCTCGAGAGGAGCAAAACCAACAAAGAACTTAATGATAAGAAGAGATTGGCAACAAGTGGAGCAAACTTTGCGAGAGCATTCACTGTTCAATTCGGAAGCTGCAAGTTCCCTGAGAATTTCACTGGCTGCCAAGATCTTGCCAAGCAAAAGAAAGTTCCATTTATCTCAGAAGATATTGCTTTGGAATGCGAAGGCAAGGACAAGTACAAGTGTGGTTCCAATGTTTTCTGGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaN

Protein Analysis

171

Amino Acids

18.43

Weight (kDa)

9.11

Isoelectric Point (pI)

33.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaN PF05479 44 - 142 6.9e-47 Photosystem I reaction centre subunit N (PSAN or PSI-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64040 AT5G64040
fragaria_vesca FvH4_1g24360
malus_domestica MD01G1004300.v1.1 MD15G1441800.v1.1
prunus_persica Prupe.4G271200_v2.0.a1
pyrus_communis pycom01g02140
rosa_chinensis RchiOBHm_Chr2g0120891
rosa_laevigata RLG00000018599
rosa_multiflora Rmu_ssc0000077.1_g000001
rosa_roxburghii Rroxscaffold_2G00122760
rosa_rugosa Rorug02G0232500
rosa_samantha Rh2AG288900 Rh2BG299900 Rh2CG277900 Rh2DG314100
rosa_wichuraiana Rw2G023140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 153
AciI CCGC 1 cut(s) 5
AclWI GGATC 1 cut(s) 184
AcoI YGGCCR 1 cut(s) 6
AcsI RAATTY 2 cut(s) 91, 391
AcyI GRCGYC 1 cut(s) 261
AfaI GTAC 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 151
AgsI TTSAA 1 cut(s) 365
AjuI GAANNNNNNNTTGG 2 cut(s) 68, 100
AluBI AGCT 5 cut(s) 36, 67, 146, 215, 375
AluI AGCT 5 cut(s) 36, 67, 146, 215, 375
AlwI GGATC 1 cut(s) 184
Ama87I CYCGRG 2 cut(s) 16, 278
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 5 cut(s) 33, 212, 233, 375, 402
ApoI RAATTY 2 cut(s) 91, 391
AsuHPI GGTGA 2 cut(s) 100, 173
AvaI CYCGRG 2 cut(s) 16, 278
BbsI GAAGAC 1 cut(s) 193
BbvI GCAGC 5 cut(s) 45, 220, 224, 362, 389
BccI CCATC 1 cut(s) 167
BclI TGATCA 1 cut(s) 138
BglII AGATCT 1 cut(s) 409
BisI GCNGC 6 cut(s) 6, 34, 213, 234, 376, 403
BlsI GCNGC 6 cut(s) 7, 35, 214, 235, 377, 404
BmeT110I CYCGRG 2 cut(s) 16, 278
BmiI GGNNCC 1 cut(s) 493
BpiI GAAGAC 1 cut(s) 193
BsaHI GRCGYC 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 198
Bse1I ACTGG 1 cut(s) 403
BseDI CCNNGG 1 cut(s) 198
BseMII CTCAG 2 cut(s) 378, 456
BseNI ACTGG 1 cut(s) 403
BseRI GAGGAG 1 cut(s) 298
BseXI GCAGC 5 cut(s) 45, 220, 224, 362, 389
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 8
BsiHKCI CYCGRG 2 cut(s) 16, 278
BsmI GAATGC 2 cut(s) 353, 467
BsnI GGCC 1 cut(s) 8
BsoBI CYCGRG 2 cut(s) 16, 278
Bsp143I GATC 4 cut(s) 138, 176, 191, 409
Bsp19I CCATGG 1 cut(s) 198
BspACI CCGC 1 cut(s) 5
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 379, 455
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 493
BspPI GGATC 1 cut(s) 184
BsrI ACTGG 1 cut(s) 403
BssECI CCNNGG 1 cut(s) 198
BssMI GATC 4 cut(s) 138, 176, 191, 409
BssNI GRCGYC 1 cut(s) 261
BssT1I CCWWGG 1 cut(s) 198
Bst4CI ACNGT 1 cut(s) 361
Bst6I CTCTTC 2 cut(s) 227, 311
BstACI GRCGYC 1 cut(s) 261
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 2 cut(s) 49, 259
BstDEI CTNAG 3 cut(s) 208, 387, 442
BstDSI CCRYGG 1 cut(s) 198
BstFNI CGCG 1 cut(s) 153
BstKTI GATC 4 cut(s) 141, 179, 194, 412
BstMBI GATC 4 cut(s) 138, 176, 191, 409
BstMWI GCNNNNNNNGC 2 cut(s) 242, 344
BstUI CGCG 1 cut(s) 153
BstV1I GCAGC 5 cut(s) 45, 220, 224, 362, 389
BstV2I GAAGAC 1 cut(s) 193
BstX2I RGATCY 2 cut(s) 176, 409
BstXI CCANNNNNNTGG 1 cut(s) 80
BstYI RGATCY 2 cut(s) 176, 409
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 198
BtsI GCAGTG 1 cut(s) 228
BtsIMutI CAGTG 3 cut(s) 228, 357, 396
Cac8I GCNNGC 2 cut(s) 49, 259
CseI GACGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 481
CviAII CATG 2 cut(s) 10, 199
CviQI GTAC 1 cut(s) 481
DdeI CTNAG 3 cut(s) 208, 387, 442
DpnI GATC 4 cut(s) 140, 178, 193, 411
DpnII GATC 4 cut(s) 138, 176, 191, 409
EaeI YGGCCR 1 cut(s) 6
Eam1104I CTCTTC 2 cut(s) 227, 311
EarI CTCTTC 2 cut(s) 227, 311
Eco130I CCWWGG 1 cut(s) 198
Eco88I CYCGRG 2 cut(s) 16, 278
EcoRI GAATTC 1 cut(s) 91
EcoT14I CCWWGG 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 198
FaeI CATG 2 cut(s) 13, 202
FaiI YATR 3 cut(s) 11, 131, 200
FatI CATG 2 cut(s) 9, 198
FbaI TGATCA 1 cut(s) 138
Fnu4HI GCNGC 6 cut(s) 6, 34, 213, 234, 376, 403
Fsp4HI GCNGC 6 cut(s) 6, 34, 213, 234, 376, 403
GluI GCNGC 6 cut(s) 6, 34, 213, 234, 376, 403
HaeIII GGCC 1 cut(s) 8
HgaI GACGC 1 cut(s) 250
Hin1I GRCGYC 1 cut(s) 261
Hin1II CATG 2 cut(s) 13, 202
HphI GGTGA 2 cut(s) 100, 173
Hpy188I TCNGA 3 cut(s) 114, 371, 445
Hpy188III TCNNGA 2 cut(s) 280, 505
HpyAV CCTTC 1 cut(s) 461
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4V TGCA 2 cut(s) 33, 378
HpyF10VI GCNNNNNNNGC 2 cut(s) 242, 344
HpyF3I CTNAG 3 cut(s) 208, 387, 442
Hsp92I GRCGYC 1 cut(s) 261
Hsp92II CATG 2 cut(s) 13, 202
Ksp22I TGATCA 1 cut(s) 138
Kzo9I GATC 4 cut(s) 138, 176, 191, 409
LmnI GCTCC 2 cut(s) 285, 335
LpnPI CCDG 8 cut(s) 33, 39, 86, 147, 243, 384, 399, 490
Lsp1109I GCAGC 5 cut(s) 45, 220, 224, 362, 389
MalI GATC 4 cut(s) 140, 178, 193, 411
MboI GATC 4 cut(s) 138, 176, 191, 409
MboII GAAGA 5 cut(s) 127, 198, 214, 328, 458
MfeI CAATTG 1 cut(s) 42
MflI RGATCY 2 cut(s) 176, 409
MluCI AATT 4 cut(s) 42, 91, 365, 391
MluI ACGCGT 1 cut(s) 151
MnlI CCTC 2 cut(s) 276, 287
MseI TTAA 2 cut(s) 69, 306
MunI CAATTG 1 cut(s) 42
Mva1269I GAATGC 2 cut(s) 353, 467
MvnI CGCG 1 cut(s) 153
MwoI GCNNNNNNNGC 2 cut(s) 242, 344
NcoI CCATGG 1 cut(s) 198
NdeII GATC 4 cut(s) 138, 176, 191, 409
NlaIII CATG 2 cut(s) 13, 202
NlaIV GGNNCC 1 cut(s) 493
PaeR7I CTCGAG 2 cut(s) 16, 278
PcsI WCGNNNNNNNCGW 1 cut(s) 276
PctI GAATGC 2 cut(s) 353, 467
PkrI GCNGC 6 cut(s) 7, 35, 214, 235, 377, 404
PspN4I GGNNCC 1 cut(s) 493
PspXI VCTCGAGB 1 cut(s) 16
PsuI RGATCY 2 cut(s) 176, 409
RsaI GTAC 1 cut(s) 482
RsaNI GTAC 1 cut(s) 481
SaqAI TTAA 2 cut(s) 69, 306
SatI GCNGC 6 cut(s) 6, 34, 213, 234, 376, 403
Sau3AI GATC 4 cut(s) 138, 176, 191, 409
SetI ASST 6 cut(s) 38, 69, 148, 217, 279, 377
Sfr274I CTCGAG 2 cut(s) 16, 278
SlaI CTCGAG 2 cut(s) 16, 278
SmlI CTYRAG 2 cut(s) 16, 278
SmoI CTYRAG 2 cut(s) 16, 278
Sse9I AATT 4 cut(s) 42, 91, 365, 391
SsiI CCGC 1 cut(s) 5
StyI CCWWGG 1 cut(s) 198
TaaI ACNGT 1 cut(s) 361
TaqI TCGA 2 cut(s) 17, 279
TasI AATT 4 cut(s) 42, 91, 365, 391
TatI WGTACW 1 cut(s) 480
TauI GCSGC 1 cut(s) 8
Tru1I TTAA 2 cut(s) 69, 306
Tru9I TTAA 2 cut(s) 69, 306
TscAI CASTG 3 cut(s) 235, 364, 403
TseI GCWGC 5 cut(s) 33, 212, 233, 375, 402
TspDTI ATGAA 2 cut(s) 26, 84
TspRI CASTG 3 cut(s) 235, 364, 403
XapI RAATTY 2 cut(s) 91, 391
XhoI CTCGAG 2 cut(s) 16, 278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.