Rroxscaffold_2G00122760

Photosystem I reaction center subunit N

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014548
Physical Location & Seq
Reverse (-)
56166531 .. 56167505
975 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00122760.1

Sequence Viewer

Length: 486 bp
ATGGCAGCCATGAACTCTAGCGTTTTGGCAAGTAACTATGCCATCTCTGGCAGCAGTGCAGTACTGGCTGAAGTCAATGGCAAGATGACCTCCATGCCTCAGAGAGTAGCCATGCCTGTAATTAGGGCCCAACATCAAGAGTCGCAAAGCGTTGGAGGAAGAAGAGCTGCACTGCTCTTCCTCACCGCCACTGCCGCAGCTGTTGCCTCCAACTCCATTGCCAATGCCAGCGTCTTTGACGACTACCTGGAGAAGAGCAAGGCCAACAAGGAACTCAATGACAGGAAGAGGCTTGCAACTAGTGGAGCAAACTTTGCAAGAGCATTCACTGTTCAATTTGGGACCTGCAAATTCCCTGAGAACTTCACTGGCTGCCAAGATCTTGCCAAGCAAAATAAAGTGCCATTTCTCTCTGACGATCTGAAGTTGGAATGTGAAGGCAAGGACAAGTTCAAGTGTGGTTCCAATGTATTCTGGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0019904
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaN

Protein Analysis

161

Amino Acids

17.44

Weight (kDa)

9.28

Isoelectric Point (pI)

40.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaN PF05479 41 - 161 3.8e-61 Photosystem I reaction centre subunit N (PSAN or PSI-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64040 AT5G64040
fragaria_vesca FvH4_1g24360
malus_domestica MD01G1004300.v1.1 MD15G1441800.v1.1
prunus_persica Prupe.4G271200_v2.0.a1
pyrus_communis pycom01g02140
rosa_chinensis RchiOBHm_Chr2g0120891
rosa_laevigata RLG00000018599
rosa_multiflora Rmu_ssc0000077.1_g000001
rosa_roxburghii Rroxscaffold_2G00122760
rosa_rugosa Rorug02G0232500
rosa_samantha Rh2AG288900 Rh2BG299900 Rh2CG277900 Rh2DG314100
rosa_wichuraiana Rw2G023140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 353
AciI CCGC 2 cut(s) 186, 195
AcsI RAATTY 1 cut(s) 350
AcuI CTGAAG 2 cut(s) 90, 443
AfaI GTAC 1 cut(s) 63
AgsI TTSAA 2 cut(s) 335, 454
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 1 cut(s) 246
AluBI AGCT 2 cut(s) 167, 200
AluI AGCT 2 cut(s) 167, 200
AoxI GGCC 2 cut(s) 126, 261
ApaI GGGCCC 1 cut(s) 130
ApeKI GCWGC 5 cut(s) 5, 51, 167, 197, 372
ApoI RAATTY 1 cut(s) 350
AspS9I GGNCC 3 cut(s) 126, 127, 342
AsuHPI GGTGA 1 cut(s) 175
AvaII GGWCC 1 cut(s) 342
BaeGI GKGCMC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 130
BbvI GCAGC 5 cut(s) 17, 63, 154, 209, 359
BccI CCATC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 248
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 2 cut(s) 18, 300
BfuAI ACCTGC 1 cut(s) 353
BglII AGATCT 1 cut(s) 379
BisI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
BlsI GCNGC 6 cut(s) 7, 53, 169, 196, 199, 374
BmcAI AGTACT 1 cut(s) 63
Bme1390I CCNGG 1 cut(s) 248
Bme18I GGWCC 1 cut(s) 342
BmgT120I GGNCC 3 cut(s) 126, 127, 342
BmiI GGNNCC 3 cut(s) 128, 343, 463
BmrFI CCNGG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 269
Bse1I ACTGG 2 cut(s) 69, 373
Bse3DI GCAATG 1 cut(s) 216
BseBI CCWGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 216
BseMII CTCAG 2 cut(s) 113, 348
BseNI ACTGG 2 cut(s) 69, 373
BseSI GKGCMC 1 cut(s) 130
BseXI GCAGC 5 cut(s) 17, 63, 154, 209, 359
BsgI GTGCAG 2 cut(s) 78, 153
BshFI GGCC 2 cut(s) 128, 263
BslFI GGGAC 1 cut(s) 355
BsmFI GGGAC 1 cut(s) 355
BsmI GAATGC 1 cut(s) 323
BsnI GGCC 2 cut(s) 128, 263
Bsp120I GGGCCC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 130
Bsp143I GATC 2 cut(s) 379, 418
BspACI CCGC 2 cut(s) 186, 195
BspANI GGCC 2 cut(s) 128, 263
BspCNI CTCAG 2 cut(s) 112, 349
BspLI GGNNCC 3 cut(s) 128, 343, 463
BspMI ACCTGC 1 cut(s) 353
BspQI GCTCTTC 3 cut(s) 157, 182, 248
BsrDI GCAATG 1 cut(s) 216
BsrI ACTGG 2 cut(s) 69, 373
BssMI GATC 2 cut(s) 379, 418
Bst2UI CCWGG 1 cut(s) 248
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 4 cut(s) 157, 182, 248, 281
BstAPI GCANNNNNTGC 2 cut(s) 203, 314
BstC8I GCNNGC 2 cut(s) 229, 294
BstDEI CTNAG 2 cut(s) 99, 357
BstKTI GATC 2 cut(s) 382, 421
BstMBI GATC 2 cut(s) 379, 418
BstMWI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
BstNI CCWGG 1 cut(s) 248
BstSCI CCNGG 1 cut(s) 246
BstSLI GKGCMC 1 cut(s) 130
BstV1I GCAGC 5 cut(s) 17, 63, 154, 209, 359
BstX2I RGATCY 1 cut(s) 379
BstYI RGATCY 1 cut(s) 379
BsuRI GGCC 2 cut(s) 128, 263
BtsI GCAGTG 3 cut(s) 61, 170, 189
BtsIMutI CAGTG 5 cut(s) 61, 170, 189, 327, 366
BveI ACCTGC 1 cut(s) 353
Cac8I GCNNGC 2 cut(s) 229, 294
Cfr13I GGNCC 3 cut(s) 126, 127, 342
CseI GACGC 1 cut(s) 220
Csp6I GTAC 1 cut(s) 62
CviAII CATG 3 cut(s) 10, 94, 112
CviJI RGCY 9 cut(s) 8, 68, 110, 128, 167, 200, 263, 292, 372
CviKI_1 RGCY 9 cut(s) 8, 68, 110, 128, 167, 200, 263, 292, 372
CviQI GTAC 1 cut(s) 62
DdeI CTNAG 2 cut(s) 99, 357
DpnI GATC 2 cut(s) 381, 420
DpnII GATC 2 cut(s) 379, 418
Eam1104I CTCTTC 4 cut(s) 157, 182, 248, 281
EarI CTCTTC 4 cut(s) 157, 182, 248, 281
Eco24I GRGCYC 1 cut(s) 130
Eco47I GGWCC 1 cut(s) 342
Eco57I CTGAAG 2 cut(s) 90, 443
EcoO109I RGGNCCY 2 cut(s) 126, 342
EcoRII CCWGG 1 cut(s) 246
EcoT38I GRGCYC 1 cut(s) 130
FaeI CATG 3 cut(s) 13, 97, 115
FaiI YATR 4 cut(s) 11, 39, 95, 113
FaqI GGGAC 1 cut(s) 355
FatI CATG 3 cut(s) 9, 93, 111
Fnu4HI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
FriOI GRGCYC 1 cut(s) 130
Fsp4HI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
FspBI CTAG 2 cut(s) 18, 300
GluI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
GsuI CTGGAG 1 cut(s) 269
HaeIII GGCC 2 cut(s) 128, 263
HgaI GACGC 1 cut(s) 220
Hin1II CATG 3 cut(s) 13, 97, 115
HinfI GANTC 1 cut(s) 140
HphI GGTGA 1 cut(s) 175
Hpy188I TCNGA 3 cut(s) 102, 415, 423
Hpy188III TCNNGA 2 cut(s) 137, 475
HpyAV CCTTC 1 cut(s) 431
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4V TGCA 5 cut(s) 59, 170, 296, 317, 348
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
HpyF3I CTNAG 2 cut(s) 99, 357
Hsp92II CATG 3 cut(s) 13, 97, 115
Kzo9I GATC 2 cut(s) 379, 418
LguI GCTCTTC 3 cut(s) 157, 182, 248
LmnI GCTCC 1 cut(s) 305
Lsp1109I GCAGC 5 cut(s) 17, 63, 154, 209, 359
MaeI CTAG 2 cut(s) 18, 300
MaeIII GTNAC 1 cut(s) 32
MalI GATC 2 cut(s) 381, 420
MboI GATC 2 cut(s) 379, 418
MboII GAAGA 5 cut(s) 169, 171, 174, 265, 298
MflI RGATCY 1 cut(s) 379
MhlI GDGCHC 1 cut(s) 130
MluCI AATT 3 cut(s) 120, 335, 350
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 3 cut(s) 133, 234, 408
MnlI CCTC 6 cut(s) 100, 108, 149, 191, 217, 282
MspA1I CMGCKG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 248
Mva1269I GAATGC 1 cut(s) 323
MvaI CCWGG 1 cut(s) 248
MwoI GCNNNNNNNGC 4 cut(s) 65, 194, 203, 314
NdeII GATC 2 cut(s) 379, 418
NlaIII CATG 3 cut(s) 13, 97, 115
NlaIV GGNNCC 3 cut(s) 128, 343, 463
PciSI GCTCTTC 3 cut(s) 157, 182, 248
PctI GAATGC 1 cut(s) 323
PkrI GCNGC 6 cut(s) 7, 53, 169, 196, 199, 374
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
PpuMI RGGWCCY 1 cut(s) 342
Psp5II RGGWCCY 1 cut(s) 342
Psp6I CCWGG 1 cut(s) 246
PspGI CCWGG 1 cut(s) 246
PspN4I GGNNCC 3 cut(s) 128, 343, 463
PspOMI GGGCCC 1 cut(s) 126
PspPI GGNCC 3 cut(s) 126, 127, 342
PspPPI RGGWCCY 1 cut(s) 342
PsuI RGATCY 1 cut(s) 379
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 1 cut(s) 63
RsaNI GTAC 1 cut(s) 62
SapI GCTCTTC 3 cut(s) 157, 182, 248
SatI GCNGC 6 cut(s) 6, 52, 168, 195, 198, 373
Sau3AI GATC 2 cut(s) 379, 418
Sau96I GGNCC 3 cut(s) 126, 127, 342
ScaI AGTACT 1 cut(s) 63
SchI GAGTC 1 cut(s) 149
ScrFI CCNGG 1 cut(s) 248
SduI GDGCHC 1 cut(s) 130
SetI ASST 5 cut(s) 92, 169, 202, 249, 347
SinI GGWCC 1 cut(s) 342
SpeI ACTAGT 1 cut(s) 299
Sse9I AATT 3 cut(s) 120, 335, 350
SsiI CCGC 2 cut(s) 186, 195
SspMI CTAG 2 cut(s) 18, 300
StyD4I CCNGG 1 cut(s) 246
TaaI ACNGT 1 cut(s) 331
TasI AATT 3 cut(s) 120, 335, 350
TatI WGTACW 1 cut(s) 61
TauI GCSGC 1 cut(s) 197
TscAI CASTG 5 cut(s) 61, 177, 196, 334, 373
TseI GCWGC 5 cut(s) 5, 51, 167, 197, 372
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 5 cut(s) 61, 177, 196, 334, 373
VpaK11BI GGWCC 1 cut(s) 342
XapI RAATTY 1 cut(s) 350
XspI CTAG 2 cut(s) 18, 300
ZrmI AGTACT 1 cut(s) 63
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.