MD01G1004300.v1.1

Photosystem I reaction center subunit N

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014548
Physical Location & Seq
Forward (+)
1359707 .. 1361011
1305 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1004300.v1.1.491

Sequence Viewer

Length: 510 bp
ATGGCAGCGATGAACTCTAGTGTGCTGGCATGCAACTATGCCGTCTCTGGCACCAGAATGTCTGAACTTGTCAATGCAAACACGACTTGCATGCCTTCAGTTGTATTGGCTGCTCAGACCAAAGTTGTAGCACTCAGGGCCCAACAGCCTAGCACACAAAATAATCAAGGAGCTGATGGAAGAAGGGCTGTGCTGCTCTCCCTGGCGGCCATTGCTTTCACCGCTTTTTCTTCCAACTCCACTGCCAATGCCGGTGTCATTGATGATTACCTCGAAAAAAGCAAAGCCAACAAGGAACTGAATGACAAGAAGAGGCTGGCAACAAGCGGTGCAAACTTTGCAAGAGCATTCACTGTTCAATTTGGATCATGCAAGTTCCCTGAGAACTTCACTGGCTGCCAAGATCTTGCCAAGCAAAAGAAAGTACCATTTCTCTCTGACGATCTTAGTTTGGAGTGCGAAGGCAAGGACAAGTACAAGTGTGGTTCCAACGTTTTCTGGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00195 ko01100 map00195 map01100
Pfam Domains
Protein Families
PsaN

Protein Analysis

170

Amino Acids

18.22

Weight (kDa)

9.07

Isoelectric Point (pI)

40.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsaN PF05479 42 - 169 1.2e-64 Photosystem I reaction centre subunit N (PSAN or PSI-N)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014548)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64040 AT5G64040
fragaria_vesca FvH4_1g24360
malus_domestica MD01G1004300.v1.1 MD15G1441800.v1.1
prunus_persica Prupe.4G271200_v2.0.a1
pyrus_communis pycom01g02140
rosa_chinensis RchiOBHm_Chr2g0120891
rosa_laevigata RLG00000018599
rosa_multiflora Rmu_ssc0000077.1_g000001
rosa_roxburghii Rroxscaffold_2G00122760
rosa_rugosa Rorug02G0232500
rosa_samantha Rh2AG288900 Rh2BG299900 Rh2CG277900 Rh2DG314100
rosa_wichuraiana Rw2G023140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 50
AciI CCGC 3 cut(s) 206, 222, 327
AclI AACGTT 1 cut(s) 492
AclWI GGATC 1 cut(s) 373
AcoI YGGCCR 1 cut(s) 207
AcuI CTGAAG 1 cut(s) 81
AfaI GTAC 2 cut(s) 426, 476
AgsI TTSAA 1 cut(s) 359
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 1 cut(s) 173
AluI AGCT 1 cut(s) 173
Alw26I GTCTC 1 cut(s) 49
AlwI GGATC 1 cut(s) 373
AoxI GGCC 2 cut(s) 138, 207
ApaI GGGCCC 1 cut(s) 142
ApeKI GCWGC 4 cut(s) 5, 110, 193, 396
AspS9I GGNCC 2 cut(s) 138, 139
AsuHPI GGTGA 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 142
BanI GGYRCC 1 cut(s) 50
BanII GRGCYC 1 cut(s) 142
BbvI GCAGC 4 cut(s) 17, 97, 180, 383
BccI CCATC 1 cut(s) 170
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 73, 107
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 49
BfaI CTAG 2 cut(s) 18, 150
BglII AGATCT 1 cut(s) 403
BisI GCNGC 5 cut(s) 6, 111, 194, 207, 397
BlsI GCNGC 5 cut(s) 7, 112, 195, 208, 398
Bme1390I CCNGG 1 cut(s) 203
BmgT120I GGNCC 2 cut(s) 138, 139
BmiI GGNNCC 3 cut(s) 52, 140, 487
BmrFI CCNGG 1 cut(s) 203
BsaJI CCNNGG 1 cut(s) 201
Bse118I RCCGGY 1 cut(s) 251
Bse1I ACTGG 1 cut(s) 397
Bse3DI GCAATG 1 cut(s) 210
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 201
BseMI GCAATG 1 cut(s) 210
BseMII CTCAG 3 cut(s) 128, 148, 372
BseNI ACTGG 1 cut(s) 397
BseSI GKGCMC 1 cut(s) 142
BseXI GCAGC 4 cut(s) 17, 97, 180, 383
BshFI GGCC 2 cut(s) 140, 209
BshNI GGYRCC 1 cut(s) 50
BsiSI CCGG 1 cut(s) 252
BsmAI GTCTC 1 cut(s) 49
BsmBI CGTCTC 1 cut(s) 49
BsmI GAATGC 1 cut(s) 347
BsnI GGCC 2 cut(s) 140, 209
Bsp120I GGGCCC 1 cut(s) 138
Bsp1286I GDGCHC 1 cut(s) 142
Bsp143I GATC 3 cut(s) 365, 403, 442
BspACI CCGC 3 cut(s) 206, 222, 327
BspANI GGCC 2 cut(s) 140, 209
BspCNI CTCAG 3 cut(s) 127, 147, 373
BspLI GGNNCC 3 cut(s) 52, 140, 487
BspPI GGATC 1 cut(s) 373
BspT107I GGYRCC 1 cut(s) 50
BsrDI GCAATG 1 cut(s) 210
BsrFI RCCGGY 1 cut(s) 251
BsrI ACTGG 1 cut(s) 397
BssAI RCCGGY 1 cut(s) 251
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 3 cut(s) 365, 403, 442
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 1 cut(s) 355
Bst6I CTCTTC 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 338
BstC8I GCNNGC 4 cut(s) 27, 31, 92, 318
BstDEI CTNAG 4 cut(s) 114, 134, 381, 446
BstKTI GATC 3 cut(s) 368, 406, 445
BstMAI GTCTC 1 cut(s) 49
BstMBI GATC 3 cut(s) 365, 403, 442
BstMWI GCNNNNNNNGC 4 cut(s) 137, 212, 221, 338
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 2 cut(s) 33, 94
BstSCI CCNGG 1 cut(s) 201
BstSLI GKGCMC 1 cut(s) 142
BstV1I GCAGC 4 cut(s) 17, 97, 180, 383
BstX2I RGATCY 1 cut(s) 403
BstYI RGATCY 1 cut(s) 403
BsuRI GGCC 2 cut(s) 140, 209
BtgZI GCGATG 1 cut(s) 23
BtsI GCAGTG 1 cut(s) 240
BtsIMutI CAGTG 3 cut(s) 240, 351, 390
Cac8I GCNNGC 4 cut(s) 27, 31, 92, 318
Cfr10I RCCGGY 1 cut(s) 251
Cfr13I GGNCC 2 cut(s) 138, 139
Csp6I GTAC 2 cut(s) 425, 475
CviAII CATG 3 cut(s) 30, 91, 369
CviJI RGCY 9 cut(s) 110, 140, 148, 173, 188, 209, 287, 316, 396
CviKI_1 RGCY 9 cut(s) 110, 140, 148, 173, 188, 209, 287, 316, 396
CviQI GTAC 2 cut(s) 425, 475
DdeI CTNAG 4 cut(s) 114, 134, 381, 446
DpnI GATC 3 cut(s) 367, 405, 444
DpnII GATC 3 cut(s) 365, 403, 442
EaeI YGGCCR 1 cut(s) 207
Eam1104I CTCTTC 1 cut(s) 305
EarI CTCTTC 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 81
EcoO109I RGGNCCY 1 cut(s) 138
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 142
Esp3I CGTCTC 1 cut(s) 49
FaeI CATG 3 cut(s) 33, 94, 372
FaiI YATR 4 cut(s) 31, 39, 92, 370
FatI CATG 3 cut(s) 29, 90, 368
Fnu4HI GCNGC 5 cut(s) 6, 111, 194, 207, 397
FriOI GRGCYC 1 cut(s) 142
Fsp4HI GCNGC 5 cut(s) 6, 111, 194, 207, 397
FspBI CTAG 2 cut(s) 18, 150
GluI GCNGC 5 cut(s) 6, 111, 194, 207, 397
HaeIII GGCC 2 cut(s) 140, 209
HapII CCGG 1 cut(s) 252
Hin1II CATG 3 cut(s) 33, 94, 372
HpaII CCGG 1 cut(s) 252
HphI GGTGA 1 cut(s) 211
Hpy188I TCNGA 3 cut(s) 64, 117, 439
Hpy188III TCNNGA 1 cut(s) 499
HpyAV CCTTC 3 cut(s) 105, 177, 455
HpyCH4III ACNGT 1 cut(s) 355
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 6 cut(s) 33, 77, 90, 332, 341, 372
HpyF10VI GCNNNNNNNGC 4 cut(s) 137, 212, 221, 338
HpyF3I CTNAG 4 cut(s) 114, 134, 381, 446
HpySE526I ACGT 1 cut(s) 492
Hsp92II CATG 3 cut(s) 33, 94, 372
Kzo9I GATC 3 cut(s) 365, 403, 442
LmnI GCTCC 1 cut(s) 170
Lsp1109I GCAGC 4 cut(s) 17, 97, 180, 383
MaeI CTAG 2 cut(s) 18, 150
MaeII ACGT 1 cut(s) 492
MalI GATC 3 cut(s) 367, 405, 444
MboI GATC 3 cut(s) 365, 403, 442
MboII GAAGA 3 cut(s) 192, 222, 322
MflI RGATCY 1 cut(s) 403
MhlI GDGCHC 1 cut(s) 142
MluCI AATT 1 cut(s) 359
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 2 cut(s) 281, 306
MslI CAYNNNNRTG 1 cut(s) 56
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 1 cut(s) 347
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 4 cut(s) 137, 212, 221, 338
NdeII GATC 3 cut(s) 365, 403, 442
NlaIII CATG 3 cut(s) 33, 94, 372
NlaIV GGNNCC 3 cut(s) 52, 140, 487
NspI RCATGY 2 cut(s) 33, 94
PaeI GCATGC 2 cut(s) 33, 94
PctI GAATGC 1 cut(s) 347
PkrI GCNGC 5 cut(s) 7, 112, 195, 208, 398
Psp1406I AACGTT 1 cut(s) 492
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 3 cut(s) 52, 140, 487
PspOMI GGGCCC 1 cut(s) 138
PspPI GGNCC 2 cut(s) 138, 139
PsuI RGATCY 1 cut(s) 403
RsaI GTAC 2 cut(s) 426, 476
RsaNI GTAC 2 cut(s) 425, 475
RseI CAYNNNNRTG 1 cut(s) 56
SatI GCNGC 5 cut(s) 6, 111, 194, 207, 397
Sau3AI GATC 3 cut(s) 365, 403, 442
Sau96I GGNCC 2 cut(s) 138, 139
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 142
SetI ASST 3 cut(s) 175, 273, 495
SmiMI CAYNNNNRTG 1 cut(s) 56
SphI GCATGC 2 cut(s) 33, 94
Sse9I AATT 1 cut(s) 359
SsiI CCGC 3 cut(s) 206, 222, 327
SspMI CTAG 2 cut(s) 18, 150
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 1 cut(s) 355
TaiI ACGT 1 cut(s) 495
TaqI TCGA 1 cut(s) 273
TasI AATT 1 cut(s) 359
TatI WGTACW 1 cut(s) 474
TauI GCSGC 1 cut(s) 209
TscAI CASTG 3 cut(s) 247, 358, 397
TseI GCWGC 4 cut(s) 5, 110, 193, 396
TspDTI ATGAA 1 cut(s) 26
TspRI CASTG 3 cut(s) 247, 358, 397
XceI RCATGY 2 cut(s) 33, 94
XspI CTAG 2 cut(s) 18, 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.