AT5G67250

F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0013203
Physical Location & Seq
Reverse (-)
26831185 .. 26833903
2719 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67250.1

Sequence Viewer

Length: 1584 bp
ATGGGCCAAGCGCCGTCGTCTACGGCGGAATCGAACGGACGAGAGTTAGATTTGAGGTTATGGTCTCCGGTGATTGTTGCTGGAGGTGAATCGATGGCTGTGGGGAATGTTGTGGATCGGGATTTCACCGGAGATCTGCCGGATGAGTGTTTAGCTCATGTGTTTCAGTTTCTTGGAGCTGGAGATCGGAAGCGATGCTCTCTTGTTTGTAAACGGTGGTTGCTCGTCGACGGTCAGAGTCGTCATCGGTTATCTCTAGATGCTAAGGATGAGATCTCCTCTTTCCTTACCTCTATGTTCAATCGATTCGATTCGGTGACGAAGCTTGCTCTCCGATGTGATCGGAAGTCTGTTAGCTTGAGCGATGAAGCTTTGGCTATGATCTCTGTTCGTTGTTTGAATCTGACTCGTGTTAAGCTTCGTGGTTGTCGCGAGATTACGGATCTGGGAATGGAAGACTTCGCTAAGAATTGTAAAAATCTTAAGAAGCTATCTGTTGGATCTTGCAATTTCGGAGCTAAAGGCGTGAACGCGATGCTCGAACATTGTAAGCTTCTGGAGGAATTGTCGGTGAAGCGACTTCGAGGAATTCATGAAGCAGCTGAGTTGATTCACCTACCTGATGATGCTTCATCTTCTTCGCTCAGGTCTATTTGCTTGAAGGAGCTCGTTAATGGCCAGGTATTTGAACCGTTGCTTGCTACTACAAGAACACTGAAAACACTGAAAATCATTCGTTGTTTGGGTGATTGGGATAAAGTTCTTCAGATGATTGCAAATGGTAAGAGTTCTTTAAGTGAAATTCACTTAGAGAGGCTCCAAGTTAGTGACATTGGGCTATCTGCGATATCTAAATGCTCAAATGTTGAGACATTGCATATAGTGAAAACTCCAGAATGTTCAAACTTTGGTCTGATTTATGTAGCTGAGAGATGTAAGCTGCTGAGGAAGCTTCATATTGATGGTTGGAGGACTAATAGGATAGGTGATGAAGGTTTACTTTCTGTAGCCAAACACTGTTTAAACTTGCAAGAACTTGTGCTGATTGGTGTTAATGCAACACATATGAGTTTAGCAGCCATTGCTTCAAACTGTGAGAAACTCGAACGGTTAGCACTTTGCGGAAGTGGAACAATAGGCGATACAGAGATTGCTTGCATCGCTAGGAAATGTGGAGCATTGAGGAAATTCTGTATTAAAGGATGTCCTGTTTCGGATCGAGGGATTGAGGCGCTCGCTGTTGGTTGTCCTAATCTGGTTAAATTGAAGGTGAAGAAATGTAAAGTTGTGACTGGAGAGATTGGAGATTGGCTGCGTGAACAAAGGAGGACACTAGTGGTGAGTATGGATGGTGATGAAACCGAAGCAGTGGTGGTGGTGGATGGTGAGGTTGAAACCGTTGTGGAGGAGCCAAGGGTGGCTCAAGCAGGTGGAATTGTGGCAGAGATTGGTTCAAGTAATGGTGGTGGAGGAAGTAGATTAGCGATGATTAGGTCAAAGTTAGGGTTTCTTGCTGGAAGAAACTTGGTAACTTGCACATTCAGAAGATGGTCTCACAATGATAATGCTAGTAGTTCTACTTGA

Protein Analysis

527

Amino Acids

57.39

Weight (kDa)

8.52

Isoelectric Point (pI)

39.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 45 - 73 7.9e-08 F-box domain
F-box-like PF12937 45 - 73 3.2e-08 F-box-like
DUF7885 PF25372 274 - 432 1.9e-09 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67250
fragaria_vesca FvH4_1g16660
malus_domestica MD02G1178100.v1.1 MD15G1288000.v1.1
prunus_persica Prupe.6G229800_v2.0.a1
pyrus_communis pycom02g14380 pycom02g14420 pycom15g25150
rosa_chinensis RchiOBHm_Chr2g0106581
rosa_laevigata RLG00000017518
rosa_multiflora Rmu_ssc0000135.1_g000051
rosa_roxburghii Rroxscaffold_2G00137000
rosa_rugosa Rorug02G0136500
rosa_samantha Rh2AG187700 Rh2BG198700 Rh2CG192700 Rh2DG193900
rosa_wichuraiana Rw2G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1418
Acc36I ACCTGC 1 cut(s) 1418
AccI GTMKAC 2 cut(s) 20, 228
AccII CGCG 2 cut(s) 432, 533
AciI CCGC 2 cut(s) 26, 1122
AclWI GGATC 4 cut(s) 123, 450, 508, 1224
AcoI YGGCCR 1 cut(s) 676
AcsI RAATTY 3 cut(s) 588, 801, 1187
AcuI CTGAAG 1 cut(s) 749
AflII CTTAAG 1 cut(s) 482
AgsI TTSAA 9 cut(s) 301, 400, 661, 689, 903, 1089, 1267, 1394, 1455
AhlI ACTAGT 1 cut(s) 1333
AjnI CCWGG 1 cut(s) 678
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 3 cut(s) 69, 863, 1557
AlwI GGATC 4 cut(s) 123, 450, 508, 1224
AoxI GGCC 2 cut(s) 4, 676
ApeKI GCWGC 4 cut(s) 599, 940, 1076, 1312
ApoI RAATTY 3 cut(s) 588, 801, 1187
AspLEI GCGC 2 cut(s) 13, 1234
AspS9I GGNCC 1 cut(s) 4
BalI TGGCCA 1 cut(s) 678
BanII GRGCYC 1 cut(s) 669
BauI CACGAG 1 cut(s) 408
BbsI GAAGAC 1 cut(s) 462
Bbv12I GWGCWC 1 cut(s) 669
BbvCI CCTCAGC 1 cut(s) 944
BbvI GCAGC 4 cut(s) 611, 927, 1088, 1299
BccI CCATC 5 cut(s) 88, 956, 1343, 1376, 1542
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 1 cut(s) 680
BcoDI GTCTC 3 cut(s) 69, 863, 1557
BcuI ACTAGT 1 cut(s) 1333
BfaI CTAG 4 cut(s) 257, 1164, 1334, 1569
BfmI CTRYAG 1 cut(s) 1005
BfoI RGCGCY 2 cut(s) 14, 1235
BfrI CTTAAG 1 cut(s) 482
BfuAI ACCTGC 1 cut(s) 1418
BglII AGATCT 2 cut(s) 133, 273
BisI GCNGC 4 cut(s) 600, 941, 1077, 1313
BlsI GCNGC 4 cut(s) 601, 942, 1078, 1314
Bme1390I CCNGG 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 818, 1410
BmrFI CCNGG 1 cut(s) 680
BmsI GCATC 5 cut(s) 185, 250, 525, 616, 1167
BpiI GAAGAC 1 cut(s) 462
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpmI CTGGAG 5 cut(s) 102, 201, 578, 876, 1314
Bpu10I CCTNAGC 3 cut(s) 264, 644, 944
BpuEI CTTGAG 2 cut(s) 379, 1407
Bsa29I ATCGAT 2 cut(s) 92, 304
BsaI GGTCTC 2 cut(s) 69, 1557
BsaJI CCNNGG 1 cut(s) 1412
BsaWI WCCGGW 2 cut(s) 67, 128
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bse1I ACTGG 1 cut(s) 1297
Bse3DI GCAATG 2 cut(s) 872, 1080
BseBI CCWGG 1 cut(s) 680
BseCI ATCGAT 2 cut(s) 92, 304
BseDI CCNNGG 1 cut(s) 1412
BseGI GGATG 5 cut(s) 148, 274, 1208, 1354, 1387
BseMI GCAATG 2 cut(s) 872, 1080
BseMII CTCAG 4 cut(s) 594, 658, 918, 935
BseNI ACTGG 1 cut(s) 1297
BseRI GAGGAG 2 cut(s) 268, 1421
BseXI GCAGC 4 cut(s) 611, 927, 1088, 1299
Bsh1236I CGCG 2 cut(s) 432, 533
BshFI GGCC 2 cut(s) 6, 678
BshVI ATCGAT 2 cut(s) 92, 304
BsiHKAI GWGCWC 1 cut(s) 669
BsiSI CCGG 3 cut(s) 68, 129, 140
BsmAI GTCTC 3 cut(s) 69, 863, 1557
BsnI GGCC 2 cut(s) 6, 678
Bso31I GGTCTC 2 cut(s) 69, 1557
Bsp1286I GDGCHC 1 cut(s) 669
Bsp143I GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
Bsp68I TCGCGA 1 cut(s) 432
BspACI CCGC 2 cut(s) 26, 1122
BspANI GGCC 2 cut(s) 6, 678
BspCNI CTCAG 4 cut(s) 595, 657, 919, 936
BspDI ATCGAT 2 cut(s) 92, 304
BspFNI CGCG 2 cut(s) 432, 533
BspHI TCATGA 1 cut(s) 592
BspLI GGNNCC 2 cut(s) 818, 1410
BspMI ACCTGC 1 cut(s) 1418
BspPI GGATC 4 cut(s) 123, 450, 508, 1224
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 2 cut(s) 69, 1557
BsrDI GCAATG 2 cut(s) 872, 1080
BsrI ACTGG 1 cut(s) 1297
BssECI CCNNGG 1 cut(s) 1412
BssMI GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
BssSI CACGAG 1 cut(s) 408
BssT1I CCWWGG 1 cut(s) 1412
Bst2BI CACGAG 1 cut(s) 408
Bst2UI CCWGG 1 cut(s) 680
Bst4CI ACNGT 7 cut(s) 216, 233, 693, 1019, 1094, 1110, 1399
BstAFI CTTAAG 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1082
BstC8I GCNNGC 4 cut(s) 327, 699, 1156, 1236
BstDEI CTNAG 7 cut(s) 264, 465, 603, 644, 808, 927, 944
BstF5I GGATG 5 cut(s) 148, 274, 1208, 1354, 1387
BstFNI CGCG 2 cut(s) 432, 533
BstH2I RGCGCY 2 cut(s) 14, 1235
BstHHI GCGC 2 cut(s) 13, 1234
BstKTI GATC 9 cut(s) 118, 136, 187, 276, 343, 384, 445, 503, 1219
BstMAI GTCTC 3 cut(s) 69, 863, 1557
BstMBI GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
BstMWI GCNNNNNNNGC 3 cut(s) 949, 1082, 1160
BstNI CCWGG 1 cut(s) 680
BstSCI CCNGG 1 cut(s) 678
BstSFI CTRYAG 1 cut(s) 1005
BstUI CGCG 2 cut(s) 432, 533
BstV1I GCAGC 4 cut(s) 611, 927, 1088, 1299
BstV2I GAAGAC 1 cut(s) 462
BstX2I RGATCY 4 cut(s) 133, 273, 442, 500
BstYI RGATCY 4 cut(s) 133, 273, 442, 500
Bsu15I ATCGAT 2 cut(s) 92, 304
BsuRI GGCC 2 cut(s) 6, 678
BsuTUI ATCGAT 2 cut(s) 92, 304
BtgZI GCGATG 5 cut(s) 208, 378, 548, 1144, 1499
BtsCI GGATG 5 cut(s) 148, 274, 1208, 1354, 1387
BtsI GCAGTG 1 cut(s) 1374
BtsIMutI CAGTG 4 cut(s) 713, 722, 1015, 1374
BtuMI TCGCGA 1 cut(s) 432
BveI ACCTGC 1 cut(s) 1418
Cac8I GCNNGC 4 cut(s) 327, 699, 1156, 1236
CciI TCATGA 1 cut(s) 592
CfoI GCGC 2 cut(s) 13, 1234
Cfr13I GGNCC 1 cut(s) 4
ClaI ATCGAT 2 cut(s) 92, 304
CviAII CATG 2 cut(s) 158, 593
DdeI CTNAG 7 cut(s) 264, 465, 603, 644, 808, 927, 944
DpnI GATC 9 cut(s) 117, 135, 186, 275, 342, 383, 444, 502, 1218
DpnII GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
DraI TTTAAA 1 cut(s) 1023
EaeI YGGCCR 1 cut(s) 676
EciI GGCGGA 1 cut(s) 41
Ecl136II GAGCTC 1 cut(s) 667
Eco130I CCWWGG 1 cut(s) 1412
Eco24I GRGCYC 1 cut(s) 669
Eco31I GGTCTC 2 cut(s) 69, 1557
Eco32I GATATC 1 cut(s) 849
Eco53kI GAGCTC 1 cut(s) 667
Eco57I CTGAAG 1 cut(s) 749
EcoICRI GAGCTC 1 cut(s) 667
EcoRI GAATTC 1 cut(s) 588
EcoRII CCWGG 1 cut(s) 678
EcoRV GATATC 1 cut(s) 849
EcoT14I CCWWGG 1 cut(s) 1412
EcoT38I GRGCYC 1 cut(s) 669
ErhI CCWWGG 1 cut(s) 1412
FaeI CATG 2 cut(s) 161, 596
FalI AAGNNNNNCTT 2 cut(s) 984, 1016
FatI CATG 2 cut(s) 157, 592
FauNDI CATATG 1 cut(s) 1065
FblI GTMKAC 2 cut(s) 20, 228
Fnu4HI GCNGC 4 cut(s) 600, 941, 1077, 1313
FokI GGATG 5 cut(s) 155, 281, 1215, 1361, 1394
FriOI GRGCYC 1 cut(s) 669
Fsp4HI GCNGC 4 cut(s) 600, 941, 1077, 1313
FspBI CTAG 4 cut(s) 257, 1164, 1334, 1569
GlaI GCGC 2 cut(s) 12, 1233
GluI GCNGC 4 cut(s) 600, 941, 1077, 1313
GsuI CTGGAG 5 cut(s) 102, 201, 578, 876, 1314
HaeII RGCGCY 2 cut(s) 14, 1235
HaeIII GGCC 2 cut(s) 6, 678
HapII CCGG 3 cut(s) 68, 129, 140
HhaI GCGC 2 cut(s) 13, 1234
Hin1II CATG 2 cut(s) 161, 596
Hin6I GCGC 2 cut(s) 11, 1232
HinP1I GCGC 2 cut(s) 11, 1232
HincII GTYRAC 1 cut(s) 229
HindII GTYRAC 1 cut(s) 229
HindIII AAGCTT 5 cut(s) 323, 369, 416, 551, 950
HinfI GANTC 8 cut(s) 29, 89, 238, 306, 311, 400, 406, 610
HpaII CCGG 3 cut(s) 68, 129, 140
Hpy166II GTNNAC 6 cut(s) 21, 212, 229, 529, 998, 1319
Hpy188III TCNNGA 6 cut(s) 119, 257, 431, 557, 593, 893
Hpy8I GTNNAC 6 cut(s) 21, 212, 229, 529, 998, 1319
Hpy99I CGWCG 3 cut(s) 19, 230, 233
HpyAV CCTTC 3 cut(s) 655, 986, 1261
HpyCH4III ACNGT 7 cut(s) 216, 233, 693, 1019, 1094, 1110, 1399
HpyCH4V TGCA 7 cut(s) 507, 776, 877, 1030, 1058, 1158, 1536
HpyF10VI GCNNNNNNNGC 3 cut(s) 949, 1082, 1160
HpyF3I CTNAG 7 cut(s) 264, 465, 603, 644, 808, 927, 944
Hsp92II CATG 2 cut(s) 161, 596
HspAI GCGC 2 cut(s) 11, 1232
Kzo9I GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
LmnI GCTCC 6 cut(s) 176, 515, 664, 822, 1175, 1408
Lsp1109I GCAGC 4 cut(s) 611, 927, 1088, 1299
LweI GCATC 5 cut(s) 185, 250, 525, 616, 1167
MaeI CTAG 4 cut(s) 257, 1164, 1334, 1569
MaeIII GTNAC 4 cut(s) 316, 827, 1288, 1528
MalI GATC 9 cut(s) 117, 135, 186, 275, 342, 383, 444, 502, 1218
MboI GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
MboII GAAGA 7 cut(s) 467, 627, 630, 755, 1285, 1530, 1557
MflI RGATCY 4 cut(s) 133, 273, 442, 500
MhlI GDGCHC 1 cut(s) 669
MlsI TGGCCA 1 cut(s) 678
MluCI AATT 8 cut(s) 469, 508, 563, 588, 801, 1187, 1262, 1434
MluNI TGGCCA 1 cut(s) 678
MlyI GAGTC 2 cut(s) 247, 400
MmeI TCCRAC 2 cut(s) 478, 947
Mox20I TGGCCA 1 cut(s) 678
MscI TGGCCA 1 cut(s) 678
MseI TTAA 8 cut(s) 414, 483, 672, 794, 1022, 1053, 1197, 1260
MslI CAYNNNNRTG 1 cut(s) 960
Msp20I TGGCCA 1 cut(s) 678
MspA1I CMGCKG 1 cut(s) 602
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 3 cut(s) 68, 129, 140
MspR9I CCNGG 1 cut(s) 680
MssI GTTTAAAC 1 cut(s) 1023
MvaI CCWGG 1 cut(s) 680
MvnI CGCG 2 cut(s) 432, 533
MwoI GCNNNNNNNGC 3 cut(s) 949, 1082, 1160
NdeI CATATG 1 cut(s) 1065
NdeII GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
NlaIII CATG 2 cut(s) 161, 596
NlaIV GGNNCC 2 cut(s) 818, 1410
NmuCI GTSAC 3 cut(s) 316, 827, 1288
NruI TCGCGA 1 cut(s) 432
PagI TCATGA 1 cut(s) 592
PaqCI CACCTGC 1 cut(s) 1418
PcsI WCGNNNNNNNCGW 2 cut(s) 29, 537
PfeI GAWTC 6 cut(s) 29, 89, 306, 311, 400, 610
PkrI GCNGC 4 cut(s) 601, 942, 1078, 1314
PleI GAGTC 2 cut(s) 246, 400
PmeI GTTTAAAC 1 cut(s) 1023
PpsI GAGTC 2 cut(s) 246, 400
Psp124BI GAGCTC 1 cut(s) 669
Psp6I CCWGG 1 cut(s) 678
PspGI CCWGG 1 cut(s) 678
PspN4I GGNNCC 2 cut(s) 818, 1410
PspPI GGNCC 1 cut(s) 4
PsuI RGATCY 4 cut(s) 133, 273, 442, 500
PvuII CAGCTG 1 cut(s) 602
RruI TCGCGA 1 cut(s) 432
RseI CAYNNNNRTG 1 cut(s) 960
SacI GAGCTC 1 cut(s) 669
SalI GTCGAC 1 cut(s) 227
SaqAI TTAA 8 cut(s) 414, 483, 672, 794, 1022, 1053, 1197, 1260
SatI GCNGC 4 cut(s) 600, 941, 1077, 1313
Sau3AI GATC 9 cut(s) 115, 133, 184, 273, 340, 381, 442, 500, 1216
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 2 cut(s) 247, 400
ScrFI CCNGG 1 cut(s) 680
SduI GDGCHC 1 cut(s) 669
SfaNI GCATC 5 cut(s) 185, 250, 525, 616, 1167
SfcI CTRYAG 1 cut(s) 1005
SgrDI CGTCGACG 1 cut(s) 227
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 3 cut(s) 358, 482, 1422
SmoI CTYRAG 3 cut(s) 358, 482, 1422
SpeI ACTAGT 1 cut(s) 1333
Sse9I AATT 8 cut(s) 469, 508, 563, 588, 801, 1187, 1262, 1434
SsiI CCGC 2 cut(s) 26, 1122
SspMI CTAG 4 cut(s) 257, 1164, 1334, 1569
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 1 cut(s) 678
StyI CCWWGG 1 cut(s) 1412
TaaI ACNGT 7 cut(s) 216, 233, 693, 1019, 1094, 1110, 1399
TaqI TCGA 9 cut(s) 32, 92, 228, 304, 309, 540, 583, 1104, 1219
TasI AATT 8 cut(s) 469, 508, 563, 588, 801, 1187, 1262, 1434
TfiI GAWTC 6 cut(s) 29, 89, 306, 311, 400, 610
Tru1I TTAA 8 cut(s) 414, 483, 672, 794, 1022, 1053, 1197, 1260
Tru9I TTAA 8 cut(s) 414, 483, 672, 794, 1022, 1053, 1197, 1260
TscAI CASTG 4 cut(s) 720, 729, 1022, 1374
TseFI GTSAC 3 cut(s) 316, 827, 1288
TseI GCWGC 4 cut(s) 599, 940, 1076, 1312
Tsp45I GTSAC 3 cut(s) 316, 827, 1288
TspDTI ATGAA 7 cut(s) 381, 581, 609, 621, 944, 1005, 1371
TspGWI ACGGA 2 cut(s) 51, 455
TspRI CASTG 4 cut(s) 720, 729, 1022, 1374
Vha464I CTTAAG 1 cut(s) 482
XapI RAATTY 3 cut(s) 588, 801, 1187
XbaI TCTAGA 1 cut(s) 256
XmiI GTMKAC 2 cut(s) 20, 228
XspI CTAG 4 cut(s) 257, 1164, 1334, 1569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.