FvH4_1g16660

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0013203
Physical Location & Seq
Reverse (-)
9644919 .. 9646517
1599 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16660.t1

Sequence Viewer

Length: 1599 bp
ATGGGGCAGTCATCTTCAATCCCCGAGACTCCACCGCAGCGAGAAGCCTCCCCCAGCCGGCGATTCAGCTTCAAGCAGCTACCGGTAATGTCAACGCCGGACCAAAACGACGACGAATCTGACCGTTCTGACACCGTCGACGGCCGCGACTACACTTTGGATCTCCCCGACGAGTGCTTGGGGATCATTTTCCATTGCCTCGGCTCCGGCGACCGGAAAAGAAGCTCCCTCGTCTGCCAGCGCTGGCTCCGCGTCGACGGCGAGCACCGCCACCGTCTCTCCCTGAGCGCGCAGGCCGGAATCTTGCCGTTCATTCCTTGCATATTCGCTCGGTTCGATTCGGTGACGAAGCTCGCGCTCCGATGTGACCGGAAATCGATCAGCATCGACGACGATTCTCTGGTCTTGATCTCCGTCCGGTGCAGGAACCTCACGCGCCTCAAGCTCCGCGGCTGCCGGGAGATCACCGACCTCGGAATGGCGGCGTTTGCTCAGAATTCCAAAGGATTGAAGAAGCTCTCCGTCGGCTCGTGCATGTTCGGCGCTAAGGCCATGAACGCCGTGCTGGAGCACTGCACGGCGCTGGAGGAGCTGTCGGTGAAGCGGCTCCGGGGGATCCACGACGCGTCGGAGAAGATCGGAGCTGGCAAAACGCCGACGACGCTGAAATCGATCTGCCTGAAGGAGATACTGAACGGTCAGTGCTTTGGACCGTTGATTGTAAGCTCGAAAAATCTCAAGACTTTGAAATTGTTTAGATGTTTGGGAGATTGGGATAGAGTTTTGGAAATGCTTGGAAATGGAAACAGGGGATTGACTGAAATTCACCTGGAGAGGATTCAAGTGACCGATTTGGGGCTTTCGGCCATTGCGAAATGCTCGAATTTGGAGATTTTGCATATTGTTAAGGCCCCCGAGTGTTCGAATTTCGGGCTTATTTGTGTTGCTGAGAATTGTAAGTTGCTTAGGAAGCTTCACATTGATGGATGGAGGACTAATAGAATCGGGGATGAGGGTTTGATTGCTGTAGCTAGGGATTGCCCCGAAATTCAAGAGCTTGTGTTGATTGGTGTAAATCCGACTTCATTAAGCTTGGCTGCAATTGCTTCAAATTGCAAGAAATTGGAGAGGTTGGCGCTTTGCGGCAGTGGAACAATTGGAGATGATGAATTCGCTTGTATTGCCGCCAAATGTGTAGCATTGAAGAAGCTGTGTATTAAGGGGTGCCCGATTTCGAATGTGGGACTTAAAACGCTTGCTTGGGGTTGCCCCAATTTGGCCAAGATTAAGGTCAAGAAGTGCAAAGGAGTGAGTGGTGAGGTTGCAGAATGGTTGCGAGAAAGGAGAGGATCACTGACTGTTAATTGGGATTCTGGAGAAATTGTACCTTTGGATGCTGCGGCTGGTTTGAGTGGAGGTGTAGAAAGCTCCATGGAGTTTCCGCTTGATCATGTAGCTGTTACCATTGCTCCATCAAGTAGCAATAATCCATTGGCATTGTTTAGGACAAAGTTTGGGTTCTTTTCAGGTAGGAGTTTTGTTCCTTGCACATTCAGAAGGTGGTCAGTTAGTGAGAATAGTAACAATGGAAACTTGTAG

Protein Analysis

533

Amino Acids

58.03

Weight (kDa)

8.79

Isoelectric Point (pI)

38.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 280 - 439 5.5e-09 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013203)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67250
fragaria_vesca FvH4_1g16660
malus_domestica MD02G1178100.v1.1 MD15G1288000.v1.1
prunus_persica Prupe.6G229800_v2.0.a1
pyrus_communis pycom02g14380 pycom02g14420 pycom15g25150
rosa_chinensis RchiOBHm_Chr2g0106581
rosa_laevigata RLG00000017518
rosa_multiflora Rmu_ssc0000135.1_g000051
rosa_roxburghii Rroxscaffold_2G00137000
rosa_rugosa Rorug02G0136500
rosa_samantha Rh2AG187700 Rh2BG198700 Rh2CG192700 Rh2DG193900
rosa_wichuraiana Rw2G014730

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1224
AccI GTMKAC 2 cut(s) 138, 255
AccII CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
AclWI GGATC 5 cut(s) 168, 191, 610, 623, 1357
AcoI YGGCCR 3 cut(s) 142, 864, 1278
AcsI RAATTY 6 cut(s) 496, 822, 883, 925, 1047, 1169
AcuI CTGAAG 1 cut(s) 701
AfaI GTAC 1 cut(s) 1386
AfeI AGCGCT 1 cut(s) 242
AfiI CCNNNNNNNGG 5 cut(s) 57, 213, 478, 855, 1276
AflIII ACRYGT 1 cut(s) 624
AgeI ACCGGT 1 cut(s) 82
AgsI TTSAA 8 cut(s) 18, 73, 511, 748, 842, 1052, 1110, 1204
AjnI CCWGG 1 cut(s) 828
Alw21I GWGCWC 2 cut(s) 267, 573
Alw26I GTCTC 2 cut(s) 20, 281
AlwI GGATC 5 cut(s) 168, 191, 610, 623, 1357
Ama87I CYCGRG 2 cut(s) 23, 914
Aor51HI AGCGCT 1 cut(s) 242
AoxI GGCC 6 cut(s) 142, 294, 549, 864, 909, 1278
ApeKI GCWGC 5 cut(s) 37, 76, 453, 1097, 1397
ApoI RAATTY 6 cut(s) 496, 822, 883, 925, 1047, 1169
AsiGI ACCGGT 1 cut(s) 82
AspLEI GCGC 8 cut(s) 243, 290, 292, 358, 438, 545, 583, 1138
AspS9I GGNCC 3 cut(s) 100, 710, 910
AsuC2I CCSGG 2 cut(s) 458, 611
AsuHPI GGTGA 5 cut(s) 355, 457, 610, 818, 1328
AsuII TTCGAA 2 cut(s) 923, 1235
AvaI CYCGRG 2 cut(s) 23, 914
AvaII GGWCC 2 cut(s) 100, 710
BaeGI GKGCMC 1 cut(s) 1229
BalI TGGCCA 1 cut(s) 1280
BamHI GGATCC 1 cut(s) 615
BanI GGYRCC 1 cut(s) 1224
BauI CACGAG 1 cut(s) 529
Bbv12I GWGCWC 2 cut(s) 267, 573
BbvI GCAGC 5 cut(s) 49, 88, 440, 1084, 1384
BccI CCATC 3 cut(s) 977, 981, 1480
BceAI ACGGC 5 cut(s) 157, 274, 292, 545, 594
BciT130I CCWGG 1 cut(s) 830
BclI TGATCA 1 cut(s) 1447
BcnI CCSGG 2 cut(s) 458, 611
BcoDI GTCTC 2 cut(s) 20, 281
BfaI CTAG 1 cut(s) 1032
BfmI CTRYAG 1 cut(s) 1026
BfoI RGCGCY 4 cut(s) 244, 546, 584, 1139
Bme1390I CCNGG 3 cut(s) 458, 611, 830
Bme18I GGWCC 2 cut(s) 100, 710
BmeT110I CYCGRG 2 cut(s) 23, 914
BmgT120I GGNCC 3 cut(s) 100, 710, 910
BmiI GGNNCC 7 cut(s) 205, 248, 428, 608, 617, 912, 1226
BmrFI CCNGG 3 cut(s) 458, 611, 830
BmsI GCATC 2 cut(s) 393, 1384
BpmI CTGGAG 4 cut(s) 587, 605, 851, 1395
Bpu10I CCTNAGC 3 cut(s) 284, 546, 965
Bpu14I TTCGAA 2 cut(s) 923, 1235
BpuEI CTTGAG 2 cut(s) 425, 722
BpuMI CCSGG 2 cut(s) 458, 611
Bsa29I ATCGAT 2 cut(s) 377, 671
BsaBI GATNNNNATC 1 cut(s) 383
BsaJI CCNNGG 5 cut(s) 199, 448, 472, 610, 1431
BsaWI WCCGGW 4 cut(s) 82, 213, 369, 417
BsaXI ACNNNNNCTCC 8 cut(s) 263, 293, 578, 608, 1368, 1398, 1453, 1483
Bsc4I CCNNNNNNNGG 5 cut(s) 57, 213, 478, 855, 1276
Bse118I RCCGGY 2 cut(s) 57, 82
Bse3DI GCAATG 3 cut(s) 193, 867, 1464
Bse8I GATNNNNATC 1 cut(s) 383
BseBI CCWGG 1 cut(s) 830
BseCI ATCGAT 2 cut(s) 377, 671
BseDI CCNNGG 5 cut(s) 199, 448, 472, 610, 1431
BseGI GGATG 3 cut(s) 992, 1015, 1399
BseJI GATNNNNATC 1 cut(s) 383
BseLI CCNNNNNNNGG 5 cut(s) 57, 213, 478, 855, 1276
BseMI GCAATG 3 cut(s) 193, 867, 1464
BseMII CTCAG 3 cut(s) 275, 506, 939
BsePI GCGCGC 1 cut(s) 288
BseRI GAGGAG 1 cut(s) 602
BseSI GKGCMC 1 cut(s) 1229
BseX3I CGGCCG 1 cut(s) 142
BseXI GCAGC 5 cut(s) 49, 88, 440, 1084, 1384
BseYI CCCAGC 1 cut(s) 53
BsgI GTGCAG 2 cut(s) 442, 559
Bsh1236I CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
Bsh1285I CGRYCG 2 cut(s) 145, 214
BshFI GGCC 6 cut(s) 144, 296, 551, 866, 911, 1280
BshNI GGYRCC 1 cut(s) 1224
BshTI ACCGGT 1 cut(s) 82
BshVI ATCGAT 2 cut(s) 377, 671
BsiEI CGRYCG 2 cut(s) 145, 214
BsiHKAI GWGCWC 2 cut(s) 267, 573
BsiHKCI CYCGRG 2 cut(s) 23, 914
BslFI GGGAC 1 cut(s) 1257
BslI CCNNNNNNNGG 5 cut(s) 57, 213, 478, 855, 1276
BsmAI GTCTC 2 cut(s) 20, 281
BsmBI CGTCTC 1 cut(s) 281
BsmFI GGGAC 1 cut(s) 1257
BsnI GGCC 6 cut(s) 144, 296, 551, 866, 911, 1280
BsoBI CYCGRG 2 cut(s) 23, 914
Bsp119I TTCGAA 2 cut(s) 923, 1235
Bsp1286I GDGCHC 3 cut(s) 267, 573, 1229
Bsp19I CCATGG 1 cut(s) 1431
BspANI GGCC 6 cut(s) 144, 296, 551, 866, 911, 1280
BspCNI CTCAG 3 cut(s) 276, 505, 940
BspDI ATCGAT 2 cut(s) 377, 671
BspFNI CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
BspLI GGNNCC 7 cut(s) 205, 248, 428, 608, 617, 912, 1226
BspPI GGATC 5 cut(s) 168, 191, 610, 623, 1357
BspT104I TTCGAA 2 cut(s) 923, 1235
BspT107I GGYRCC 1 cut(s) 1224
BsrDI GCAATG 3 cut(s) 193, 867, 1464
BsrFI RCCGGY 2 cut(s) 57, 82
BssAI RCCGGY 2 cut(s) 57, 82
BssECI CCNNGG 5 cut(s) 199, 448, 472, 610, 1431
BssHII GCGCGC 1 cut(s) 288
BssSI CACGAG 1 cut(s) 529
BssT1I CCWWGG 1 cut(s) 1431
Bst2BI CACGAG 1 cut(s) 529
Bst2UI CCWGG 1 cut(s) 830
Bst4CI ACNGT 6 cut(s) 125, 136, 275, 698, 714, 1360
BstBI TTCGAA 2 cut(s) 923, 1235
BstC8I GCNNGC 9 cut(s) 59, 239, 245, 263, 290, 294, 354, 646, 1257
BstDEI CTNAG 5 cut(s) 284, 492, 546, 948, 965
BstDSI CCRYGG 2 cut(s) 448, 1431
BstF5I GGATG 3 cut(s) 992, 1015, 1399
BstFNI CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
BstH2I RGCGCY 4 cut(s) 244, 546, 584, 1139
BstHHI GCGC 8 cut(s) 243, 290, 292, 358, 438, 545, 583, 1138
BstMAI GTCTC 2 cut(s) 20, 281
BstMCI CGRYCG 2 cut(s) 145, 214
BstNI CCWGG 1 cut(s) 830
BstNSI RCATGY 1 cut(s) 538
BstSCI CCNGG 3 cut(s) 456, 609, 828
BstSFI CTRYAG 1 cut(s) 1026
BstSLI GKGCMC 1 cut(s) 1229
BstUI CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
BstV1I GCAGC 5 cut(s) 49, 88, 440, 1084, 1384
BstX2I RGATCY 2 cut(s) 160, 615
BstYI RGATCY 2 cut(s) 160, 615
BstZI CGGCCG 1 cut(s) 142
Bsu15I ATCGAT 2 cut(s) 377, 671
BsuRI GGCC 6 cut(s) 144, 296, 551, 866, 911, 1280
BsuTUI ATCGAT 2 cut(s) 377, 671
BtgI CCRYGG 2 cut(s) 448, 1431
BtsCI GGATG 3 cut(s) 992, 1015, 1399
BtsI GCAGTG 2 cut(s) 571, 1153
BtsIMutI CAGTG 4 cut(s) 571, 707, 1153, 1352
Cac8I GCNNGC 9 cut(s) 59, 239, 245, 263, 290, 294, 354, 646, 1257
CfoI GCGC 8 cut(s) 243, 290, 292, 358, 438, 545, 583, 1138
Cfr10I RCCGGY 2 cut(s) 57, 82
Cfr13I GGNCC 3 cut(s) 100, 710, 910
Cfr42I CCGCGG 1 cut(s) 451
ClaI ATCGAT 2 cut(s) 377, 671
CseI GACGC 4 cut(s) 241, 615, 632, 670
Csp6I GTAC 1 cut(s) 1385
CspAI ACCGGT 1 cut(s) 82
CviAII CATG 4 cut(s) 535, 553, 1432, 1451
CviQI GTAC 1 cut(s) 1385
DdeI CTNAG 5 cut(s) 284, 492, 546, 948, 965
EaeI YGGCCR 3 cut(s) 142, 864, 1278
EagI CGGCCG 1 cut(s) 142
EclXI CGGCCG 1 cut(s) 142
Eco130I CCWWGG 1 cut(s) 1431
Eco47I GGWCC 2 cut(s) 100, 710
Eco47III AGCGCT 1 cut(s) 242
Eco52I CGGCCG 1 cut(s) 142
Eco57I CTGAAG 1 cut(s) 701
Eco88I CYCGRG 2 cut(s) 23, 914
EcoO109I RGGNCCY 1 cut(s) 910
EcoRI GAATTC 2 cut(s) 496, 1169
EcoRII CCWGG 1 cut(s) 828
EcoT14I CCWWGG 1 cut(s) 1431
ErhI CCWWGG 1 cut(s) 1431
Esp3I CGTCTC 1 cut(s) 281
FaeI CATG 4 cut(s) 538, 556, 1435, 1454
FaiI YATR 6 cut(s) 323, 536, 554, 900, 1433, 1452
FaqI GGGAC 1 cut(s) 1257
FatI CATG 4 cut(s) 534, 552, 1431, 1450
FbaI TGATCA 1 cut(s) 1447
FblI GTMKAC 2 cut(s) 138, 255
FokI GGATG 3 cut(s) 999, 1022, 1406
FspBI CTAG 1 cut(s) 1032
GlaI GCGC 8 cut(s) 242, 289, 291, 357, 437, 544, 582, 1137
GsaI CCCAGC 1 cut(s) 57
GsuI CTGGAG 4 cut(s) 587, 605, 851, 1395
HaeII RGCGCY 4 cut(s) 244, 546, 584, 1139
HaeIII GGCC 6 cut(s) 144, 296, 551, 866, 911, 1280
HgaI GACGC 4 cut(s) 241, 615, 632, 670
HhaI GCGC 8 cut(s) 243, 290, 292, 358, 438, 545, 583, 1138
Hin1II CATG 4 cut(s) 538, 556, 1435, 1454
Hin6I GCGC 8 cut(s) 241, 288, 290, 356, 436, 543, 581, 1136
HinP1I GCGC 8 cut(s) 241, 288, 290, 356, 436, 543, 581, 1136
HincII GTYRAC 3 cut(s) 93, 139, 256
HindII GTYRAC 3 cut(s) 93, 139, 256
HindIII AAGCTT 2 cut(s) 971, 1090
HinfI GANTC 9 cut(s) 28, 63, 116, 300, 338, 395, 838, 1002, 1370
HphI GGTGA 5 cut(s) 355, 457, 610, 818, 1328
Hpy166II GTNNAC 3 cut(s) 93, 139, 256
Hpy188I TCNGA 9 cut(s) 121, 130, 362, 476, 495, 631, 641, 1080, 1556
Hpy188III TCNNGA 5 cut(s) 406, 739, 1052, 1294, 1374
Hpy8I GTNNAC 3 cut(s) 93, 139, 256
HpyAV CCTTC 2 cut(s) 676, 1551
HpyCH4III ACNGT 6 cut(s) 125, 136, 275, 698, 714, 1360
HpyF3I CTNAG 5 cut(s) 284, 492, 546, 948, 965
Hsp92II CATG 4 cut(s) 538, 556, 1435, 1454
HspAI GCGC 8 cut(s) 241, 288, 290, 356, 436, 543, 581, 1136
KroI GCCGGC 1 cut(s) 57
KroNI GCCGGC 1 cut(s) 59
Ksp22I TGATCA 1 cut(s) 1447
KspI CCGCGG 1 cut(s) 451
Lsp1109I GCAGC 5 cut(s) 49, 88, 440, 1084, 1384
LweI GCATC 2 cut(s) 393, 1384
MaeI CTAG 1 cut(s) 1032
MaeIII GTNAC 5 cut(s) 343, 365, 844, 1459, 1580
MboII GAAGA 4 cut(s) 6, 523, 646, 1216
MfeI CAATTG 2 cut(s) 1101, 1155
MflI RGATCY 2 cut(s) 160, 615
MhlI GDGCHC 3 cut(s) 267, 573, 1229
MlsI TGGCCA 1 cut(s) 1280
MluI ACGCGT 1 cut(s) 624
MluNI TGGCCA 1 cut(s) 1280
MlyI GAGTC 1 cut(s) 22
MmeI TCCRAC 2 cut(s) 609, 1103
Mox20I TGGCCA 1 cut(s) 1280
MroNI GCCGGC 1 cut(s) 57
MscI TGGCCA 1 cut(s) 1280
MseI TTAA 6 cut(s) 906, 1088, 1218, 1248, 1287, 1362
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 1 cut(s) 1280
MspA1I CMGCKG 1 cut(s) 450
MspR9I CCNGG 3 cut(s) 458, 611, 830
MunI CAATTG 2 cut(s) 1101, 1155
MvaI CCWGG 1 cut(s) 830
MvnI CGCG 7 cut(s) 147, 252, 290, 356, 436, 450, 626
NaeI GCCGGC 1 cut(s) 59
NciI CCSGG 2 cut(s) 458, 611
NcoI CCATGG 1 cut(s) 1431
NgoMIV GCCGGC 1 cut(s) 57
NlaIII CATG 4 cut(s) 538, 556, 1435, 1454
NlaIV GGNNCC 7 cut(s) 205, 248, 428, 608, 617, 912, 1226
NmeAIII GCCGAG 1 cut(s) 180
NmuCI GTSAC 3 cut(s) 343, 365, 844
NspI RCATGY 1 cut(s) 538
NspV TTCGAA 2 cut(s) 923, 1235
PauI GCGCGC 1 cut(s) 288
PcsI WCGNNNNNNNCGW 5 cut(s) 144, 207, 333, 668, 869
PdiI GCCGGC 1 cut(s) 59
PfeI GAWTC 8 cut(s) 63, 116, 300, 338, 395, 838, 1002, 1370
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 82
PleI GAGTC 1 cut(s) 22
PpsI GAGTC 1 cut(s) 22
Psp6I CCWGG 1 cut(s) 828
PspFI CCCAGC 1 cut(s) 53
PspGI CCWGG 1 cut(s) 828
PspN4I GGNNCC 7 cut(s) 205, 248, 428, 608, 617, 912, 1226
PspPI GGNCC 3 cut(s) 100, 710, 910
PsuI RGATCY 2 cut(s) 160, 615
PsyI GACNNNGTC 1 cut(s) 134
PteI GCGCGC 1 cut(s) 288
RsaI GTAC 1 cut(s) 1386
RsaNI GTAC 1 cut(s) 1385
RseI CAYNNNNRTG 1 cut(s) 981
SacII CCGCGG 1 cut(s) 451
SalI GTCGAC 2 cut(s) 137, 254
SaqAI TTAA 6 cut(s) 906, 1088, 1218, 1248, 1287, 1362
Sau96I GGNCC 3 cut(s) 100, 710, 910
SchI GAGTC 1 cut(s) 22
ScrFI CCNGG 3 cut(s) 458, 611, 830
SduI GDGCHC 3 cut(s) 267, 573, 1229
SfaNI GCATC 2 cut(s) 393, 1384
SfcI CTRYAG 1 cut(s) 1026
Sfr303I CCGCGG 1 cut(s) 451
SfuI TTCGAA 2 cut(s) 923, 1235
SgrBI CCGCGG 1 cut(s) 451
SgrDI CGTCGACG 2 cut(s) 137, 254
SinI GGWCC 2 cut(s) 100, 710
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 2 cut(s) 440, 737
SmoI CTYRAG 2 cut(s) 440, 737
SspMI CTAG 1 cut(s) 1032
StyD4I CCNGG 3 cut(s) 456, 609, 828
StyI CCWWGG 1 cut(s) 1431
TaaI ACNGT 6 cut(s) 125, 136, 275, 698, 714, 1360
TaqII GACCGA 1 cut(s) 863
TauI GCSGC 7 cut(s) 147, 453, 485, 607, 1146, 1187, 1403
TfiI GAWTC 8 cut(s) 63, 116, 300, 338, 395, 838, 1002, 1370
Tru1I TTAA 6 cut(s) 906, 1088, 1218, 1248, 1287, 1362
Tru9I TTAA 6 cut(s) 906, 1088, 1218, 1248, 1287, 1362
TscAI CASTG 4 cut(s) 578, 707, 1153, 1359
TseFI GTSAC 3 cut(s) 343, 365, 844
TseI GCWGC 5 cut(s) 37, 76, 453, 1097, 1397
Tsp45I GTSAC 3 cut(s) 343, 365, 844
TspDTI ATGAA 4 cut(s) 301, 569, 1074, 1182
TspGWI ACGGA 2 cut(s) 403, 511
TspRI CASTG 4 cut(s) 578, 707, 1153, 1359
Tth111I GACNNNGTC 1 cut(s) 134
VpaK11BI GGWCC 2 cut(s) 100, 710
XapI RAATTY 6 cut(s) 496, 822, 883, 925, 1047, 1169
XceI RCATGY 1 cut(s) 538
XmiI GTMKAC 2 cut(s) 138, 255
XspI CTAG 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.