Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0013203

Physical Location & Seq

Genomic Coordinates Reverse (-)

15733658 .. 15735280

Gene Structure 1623 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1178100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1623 bp

ATGGGACAGTCCTATTCGTTTCCTGAGTCTCCAGCGGAGCACAGTCCAAGAGAAATATCAACGAGCCGCCGTTTCGGCTTCAAATCGGTGGCGATTGGTTCATCCTTGAACGACGAAGACTCCGAGTTTCACGGGGAAGTCACCCAGGGCCGTGACTACACCTCCGATCTTCCCGACGAGTTGTTGGCGAGTATTTTTCACTTCCTCGGTGCCGGAGACCGGAAACGAAGCTCGCTCGTCTGCCACCGGTGGCTGAGAGTCGACGGCCAGAGCCGGCACCGCCTCTCTCTCAACGCCCAGGCCGGGCTCCTCACTTTCCTGCCTGCTCTCTTCGCCCGCTTCGACTCGGTCACGAAGCTCGCTCTTCGATGCGATCGCAAATCGATCAGCCTGGACGACGACACTTTGGTCCTGATCTCGATTCGCTGCAGAAACCTTACGCGCCTCAAGCTCCGCGGCTGCCGGGAAATCACGGACCTCGGAGTGACCGCGTTCGCGCAGAACTGTAAGGGCCTGAAGAAGCTTTCCTGCGGCTCCTGCATGTTCGGCGCCAAAGCCATGAACGCCGTCTTGGAGCACTGCCCGGCGCTCGAGGAGCTCTCCGTGAAGCGGCTCCGCGGTGTCCACGACGGCTCGGAGCCGATCGGCGACAAAATTGCGTCGTCGTCGCTGAAATCGATAACCTTGAGGGAGATACTGAACGGACAGTGCTTCGGGCCGCTTATCGTCGGCTCCAAGAATCTCAAGACCCTGAAGCTAATCCGCTGTTTGGGGGATTGGGATACGGTTCTGGAAAAGCTTGAAAATGGAAACCAGAGTTTGATTGAAGTTCATCTGGAGAGGTTGCAGGTGACCGATTTGGGGCTTTCGGCAATTTCGAAATGCTCGAATTTGGAGGTTTTGCACATTGTTAAGGCTCCTGAGTGTTCAAATTTCGGGCTTATCTGTGTTGCCGAAAACTGCAAGATGCTGAGGAAGCTTCACATTGATGGATGGAGGACGAATAGGATTGGGGACGAAGGTTTGATTGCCATAGCCAAAGAGTGCCCAAACATACTGGAGCTTGTTCTGATTGGTGTGAATCCTACCTCTTTGAGCCTAACTGCAATTGCCTCCAATTGCCAAAAATTGGAGAGGTTGGCGCTCTGCGGTAGTGGATCAATTGGAGATGCAGAGTTCGCTTGCATTGCTGCGAAATGCGTGGCGTTGAAGAAACTGTGTATTAAGGGGTGCCCAATTTCCGATGTCGGGCTCGAAATGCTTGCTTGGGGTTGTCCCAGTTTGGCCAAGATTAAGGTCAAGAAGTGCAGAGGAGTGAGTGGCGAGGTGGCGGAGTGGTTGAGGGAACGAAGGGGATCATTGATTGTGAATTGGGATGCTGGTGATATTGGATCAATGGATGCTAGTGGCTATGCAGGTGGAGCTGTACAAAGCGATGTGGAGTTTCCGGTTGATCACGGAGCTGTTGCCATTGCTTCAGCAAGTAGCAATGCTCCAGCAAGTAGCAATACTCCAATGGCATTATTTAGAACAAAGTTTGGTTTTTTTTCAGGTAGGAGTTTTGTTCCATGCACATTCAGAAGGTGGTCAGTTAGTCAGAGTAGTTCCAGTGCAAACTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006464 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0070647 GO:0071704 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box F-box-like LRR_6

Protein Analysis

541

Amino Acids

58.48

Weight (kDa)

8.59

Isoelectric Point (pI)

42.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box-like	PF12937	55 - 86	3.7e-07	F-box-like
F-box	PF00646	55 - 86	5.7e-06	F-box domain
LRR_At5g56370	PF24758	134 - 229	2e-06	FBD-associated F-box protein At5g56370, LRR repeats

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013203)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67250
fragaria_vesca	FvH4_1g16660
malus_domestica	MD02G1178100.v1.1 MD15G1288000.v1.1
prunus_persica	Prupe.6G229800_v2.0.a1
pyrus_communis	pycom02g14380 pycom02g14420 pycom15g25150
rosa_chinensis	RchiOBHm_Chr2g0106581
rosa_laevigata	RLG00000017518
rosa_multiflora	Rmu_ssc0000135.1_g000051
rosa_roxburghii	Rroxscaffold_2G00137000
rosa_rugosa	Rorug02G0136500
rosa_samantha	Rh2AG187700 Rh2BG198700 Rh2CG192700 Rh2DG193900
rosa_wichuraiana	Rw2G014730

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	838, 1406
AasI	GACNNNNNNGTC	1 cut(s)	407
Acc36I	ACCTGC	2 cut(s)	838, 1406
AccB1I	GGYRCC	4 cut(s)	209, 276, 548, 1230
AccB7I	CCANNNNNTGG	1 cut(s)	1129
AccI	GTMKAC	1 cut(s)	261
AccII	CGCG	5 cut(s)	442, 456, 491, 497, 618
AclWI	GGATC	3 cut(s)	1165, 1363, 1399
AcoI	YGGCCR	2 cut(s)	265, 1284
AcsI	RAATTY	2 cut(s)	889, 931
AcuI	CTGAAG	3 cut(s)	536, 773, 1461
AcyI	GRCGYC	1 cut(s)	549
AfaI	GTAC	1 cut(s)	1428
AfiI	CCNNNNNNNGG	7 cut(s)	219, 303, 609, 769, 861, 1129, 1248
AgeI	ACCGGT	1 cut(s)	246
AgsI	TTSAA	6 cut(s)	82, 109, 803, 827, 930, 1210
AjnI	CCWGG	3 cut(s)	144, 297, 390
AjuI	GAANNNNNNNTTGG	2 cut(s)	554, 586
Alw21I	GWGCWC	3 cut(s)	42, 579, 600
Alw26I	GTCTC	2 cut(s)	33, 210
AlwI	GGATC	3 cut(s)	1165, 1363, 1399
Ama87I	CYCGRG	1 cut(s)	590
AoxI	GGCC	6 cut(s)	148, 265, 300, 511, 716, 1284
ApeKI	GCWGC	3 cut(s)	426, 459, 1190
ApoI	RAATTY	2 cut(s)	889, 931
AsiGI	ACCGGT	1 cut(s)	246
AsiSI	GCGATCGC	1 cut(s)	376
AspLEI	GCGC	5 cut(s)	444, 499, 551, 589, 1144
AspS9I	GGNCC	5 cut(s)	148, 409, 475, 511, 716
AsuC2I	CCSGG	3 cut(s)	304, 464, 584
AsuHPI	GGTGA	3 cut(s)	133, 862, 1394
AsuII	TTCGAA	1 cut(s)	878
AvaI	CYCGRG	1 cut(s)	590
AvaII	GGWCC	2 cut(s)	409, 475
BaeGI	GKGCMC	2 cut(s)	1049, 1235
BalI	TGGCCA	1 cut(s)	1286
BanI	GGYRCC	4 cut(s)	209, 276, 548, 1230
BanII	GRGCYC	3 cut(s)	309, 600, 1254
BbsI	GAAGAC	1 cut(s)	123
Bbv12I	GWGCWC	3 cut(s)	42, 579, 600
BbvCI	CCTCAGC	1 cut(s)	971
BbvI	GCAGC	3 cut(s)	413, 446, 1177
BccI	CCATC	2 cut(s)	983, 987
BceAI	ACGGC	5 cut(s)	54, 135, 280, 551, 646
BcgI	CGANNNNNNTGC	4 cut(s)	638, 672, 1244, 1278
BciT130I	CCWGG	3 cut(s)	146, 299, 392
BciVI	GTATCC	1 cut(s)	775
BclI	TGATCA	1 cut(s)	1453
BcnI	CCSGG	3 cut(s)	304, 464, 584
BcoDI	GTCTC	2 cut(s)	33, 210
BfaI	CTAG	1 cut(s)	1404
BfmI	CTRYAG	1 cut(s)	427
BfoI	RGCGCY	3 cut(s)	552, 590, 1145
BfuAI	ACCTGC	2 cut(s)	838, 1406
BfuI	GTATCC	1 cut(s)	775
BglI	GCCNNNNNGGC	1 cut(s)	75
BisI	GCNGC	8 cut(s)	67, 427, 457, 460, 532, 611, 719, 1191
BlsI	GCNGC	8 cut(s)	68, 428, 458, 461, 533, 612, 720, 1192
Bme1390I	CCNGG	6 cut(s)	146, 299, 304, 392, 464, 584
Bme18I	GGWCC	2 cut(s)	409, 475
BmeT110I	CYCGRG	1 cut(s)	590
BmgT120I	GGNCC	5 cut(s)	148, 409, 475, 511, 716
BmrFI	CCNGG	6 cut(s)	146, 299, 304, 392, 464, 584
BmrI	ACTGGG	1 cut(s)	1272
BmsI	GCATC	5 cut(s)	359, 957, 1159, 1366, 1390
BmuI	ACTGGG	1 cut(s)	1272
BpiI	GAAGAC	1 cut(s)	123
BplI	GAGNNNNNCTC	2 cut(s)	584, 616
BpmI	CTGGAG	4 cut(s)	15, 857, 1079, 1479
Bpu10I	CCTNAGC	1 cut(s)	971
Bpu14I	TTCGAA	1 cut(s)	878
BpuEI	CTTGAG	3 cut(s)	431, 706, 728
BpuMI	CCSGG	3 cut(s)	304, 464, 584
Bsa29I	ATCGAT	2 cut(s)	383, 677
BsaHI	GRCGYC	1 cut(s)	549
BsaI	GGTCTC	1 cut(s)	210
BsaJI	CCNNGG	7 cut(s)	144, 145, 205, 297, 454, 478, 616
BsaWI	WCCGGW	3 cut(s)	219, 246, 1447
BsaXI	ACNNNNNCTCC	4 cut(s)	104, 134, 146, 176
Bsc4I	CCNNNNNNNGG	7 cut(s)	219, 303, 609, 769, 861, 1129, 1248
Bse118I	RCCGGY	2 cut(s)	246, 273
Bse1I	ACTGG	3 cut(s)	1062, 1278, 1608
Bse3DI	GCAATG	3 cut(s)	1185, 1470, 1495
BseBI	CCWGG	3 cut(s)	146, 299, 392
BseCI	ATCGAT	2 cut(s)	383, 677
BseDI	CCNNGG	7 cut(s)	144, 145, 205, 297, 454, 478, 616
BseGI	GGATG	4 cut(s)	101, 998, 1381, 1405
BseLI	CCNNNNNNNGG	7 cut(s)	219, 303, 609, 769, 861, 1129, 1248
BseMI	GCAATG	3 cut(s)	1185, 1470, 1495
BseMII	CTCAG	4 cut(s)	15, 245, 912, 962
BseNI	ACTGG	3 cut(s)	1062, 1278, 1608
BseRI	GAGGAG	3 cut(s)	299, 608, 1326
BseSI	GKGCMC	2 cut(s)	1049, 1235
BseXI	GCAGC	3 cut(s)	413, 446, 1177
BsgI	GTGCAG	1 cut(s)	1327
Bsh1236I	CGCG	5 cut(s)	442, 456, 491, 497, 618
Bsh1285I	CGRYCG	2 cut(s)	376, 645
BshFI	GGCC	6 cut(s)	150, 267, 302, 513, 718, 1286
BshNI	GGYRCC	4 cut(s)	209, 276, 548, 1230
BshTI	ACCGGT	1 cut(s)	246
BshVI	ATCGAT	2 cut(s)	383, 677
BsiEI	CGRYCG	2 cut(s)	376, 645
BsiHKAI	GWGCWC	3 cut(s)	42, 579, 600
BsiHKCI	CYCGRG	1 cut(s)	590
BsiSI	CCGG	8 cut(s)	213, 220, 247, 274, 303, 463, 584, 1448
BslFI	GGGAC	3 cut(s)	18, 1028, 1260
BslI	CCNNNNNNNGG	7 cut(s)	219, 303, 609, 769, 861, 1129, 1248
BsmAI	GTCTC	2 cut(s)	33, 210
BsmFI	GGGAC	3 cut(s)	18, 1028, 1260
BsnI	GGCC	6 cut(s)	150, 267, 302, 513, 718, 1286
Bso31I	GGTCTC	1 cut(s)	210
BsoBI	CYCGRG	1 cut(s)	590
Bsp119I	TTCGAA	1 cut(s)	878
Bsp1286I	GDGCHC	7 cut(s)	42, 309, 579, 600, 1049, 1235, 1254
Bsp1407I	TGTACA	1 cut(s)	1426
Bsp143I	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
BspANI	GGCC	6 cut(s)	150, 267, 302, 513, 718, 1286
BspCNI	CTCAG	4 cut(s)	16, 246, 913, 963
BspDI	ATCGAT	2 cut(s)	383, 677
BspFNI	CGCG	5 cut(s)	442, 456, 491, 497, 618
BspMAI	CTGCAG	1 cut(s)	431
BspMI	ACCTGC	2 cut(s)	838, 1406
BspPI	GGATC	3 cut(s)	1165, 1363, 1399
BspQI	GCTCTTC	1 cut(s)	369
BspT104I	TTCGAA	1 cut(s)	878
BspT107I	GGYRCC	4 cut(s)	209, 276, 548, 1230
BspTNI	GGTCTC	1 cut(s)	210
BsrDI	GCAATG	3 cut(s)	1185, 1470, 1495
BsrFI	RCCGGY	2 cut(s)	246, 273
BsrGI	TGTACA	1 cut(s)	1426
BsrI	ACTGG	3 cut(s)	1062, 1278, 1608
BssAI	RCCGGY	2 cut(s)	246, 273
BssECI	CCNNGG	7 cut(s)	144, 145, 205, 297, 454, 478, 616
BssMI	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
BssNI	GRCGYC	1 cut(s)	549
Bst2UI	CCWGG	3 cut(s)	146, 299, 392
Bst4CI	ACNGT	6 cut(s)	9, 44, 506, 708, 787, 1218
Bst6I	CTCTTC	2 cut(s)	335, 369
BstACI	GRCGYC	1 cut(s)	549
BstAUI	TGTACA	1 cut(s)	1426
BstBI	TTCGAA	1 cut(s)	878
BstC8I	GCNNGC	7 cut(s)	233, 275, 324, 337, 360, 1183, 1263
BstDEI	CTNAG	4 cut(s)	24, 254, 921, 971
BstDSI	CCRYGG	2 cut(s)	454, 616
BstEII	GGTNACC	1 cut(s)	850
BstF5I	GGATG	4 cut(s)	101, 998, 1381, 1405
BstFNI	CGCG	5 cut(s)	442, 456, 491, 497, 618
BstH2I	RGCGCY	3 cut(s)	552, 590, 1145
BstHHI	GCGC	5 cut(s)	444, 499, 551, 589, 1144
BstKTI	GATC	9 cut(s)	169, 376, 387, 417, 645, 1160, 1358, 1394, 1456
BstMAI	GTCTC	2 cut(s)	33, 210
BstMBI	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
BstMCI	CGRYCG	2 cut(s)	376, 645
BstNI	CCWGG	3 cut(s)	146, 299, 392
BstNSI	RCATGY	1 cut(s)	544
BstPI	GGTNACC	1 cut(s)	850
BstSCI	CCNGG	6 cut(s)	144, 297, 302, 390, 462, 582
BstSFI	CTRYAG	1 cut(s)	427
BstSLI	GKGCMC	2 cut(s)	1049, 1235
BstUI	CGCG	5 cut(s)	442, 456, 491, 497, 618
BstV1I	GCAGC	3 cut(s)	413, 446, 1177
BstV2I	GAAGAC	1 cut(s)	123
Bsu15I	ATCGAT	2 cut(s)	383, 677
BsuI	GTATCC	1 cut(s)	775
BsuRI	GGCC	6 cut(s)	150, 267, 302, 513, 718, 1286
BsuTUI	ATCGAT	2 cut(s)	383, 677
BtgI	CCRYGG	2 cut(s)	454, 616
BtgZI	GCGATG	1 cut(s)	1449
BtsCI	GGATG	4 cut(s)	101, 998, 1381, 1405
BtsI	GCAGTG	1 cut(s)	577
BtsIMutI	CAGTG	3 cut(s)	577, 713, 1615
BveI	ACCTGC	2 cut(s)	838, 1406
Cac8I	GCNNGC	7 cut(s)	233, 275, 324, 337, 360, 1183, 1263
CfoI	GCGC	5 cut(s)	444, 499, 551, 589, 1144
Cfr10I	RCCGGY	2 cut(s)	246, 273
Cfr13I	GGNCC	5 cut(s)	148, 409, 475, 511, 716
Cfr42I	CCGCGG	2 cut(s)	457, 619
ClaI	ATCGAT	2 cut(s)	383, 677
CseI	GACGC	1 cut(s)	648
Csp6I	GTAC	1 cut(s)	1427
CspAI	ACCGGT	1 cut(s)	246
CviAII	CATG	3 cut(s)	541, 559, 1569
CviQI	GTAC	1 cut(s)	1427
DdeI	CTNAG	4 cut(s)	24, 254, 921, 971
DinI	GGCGCC	1 cut(s)	550
DpnI	GATC	9 cut(s)	168, 375, 386, 416, 644, 1159, 1357, 1393, 1455
DpnII	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
DrdI	GACNNNNNNGTC	1 cut(s)	407
DseDI	GACNNNNNNGTC	1 cut(s)	407
EaeI	YGGCCR	2 cut(s)	265, 1284
Eam1104I	CTCTTC	2 cut(s)	335, 369
EarI	CTCTTC	2 cut(s)	335, 369
EciI	GGCGGA	1 cut(s)	1346
Ecl136II	GAGCTC	1 cut(s)	598
Eco24I	GRGCYC	3 cut(s)	309, 600, 1254
Eco31I	GGTCTC	1 cut(s)	210
Eco47I	GGWCC	2 cut(s)	409, 475
Eco53kI	GAGCTC	1 cut(s)	598
Eco57I	CTGAAG	3 cut(s)	536, 773, 1461
Eco88I	CYCGRG	1 cut(s)	590
Eco91I	GGTNACC	1 cut(s)	850
EcoICRI	GAGCTC	1 cut(s)	598
EcoO109I	RGGNCCY	1 cut(s)	511
EcoO65I	GGTNACC	1 cut(s)	850
EcoRII	CCWGG	3 cut(s)	144, 297, 390
EcoT38I	GRGCYC	3 cut(s)	309, 600, 1254
EgeI	GGCGCC	1 cut(s)	550
EheI	GGCGCC	1 cut(s)	550
FaeI	CATG	3 cut(s)	544, 562, 1572
FaiI	YATR	6 cut(s)	542, 560, 1034, 1055, 1413, 1570
FaqI	GGGAC	3 cut(s)	18, 1028, 1260
FatI	CATG	3 cut(s)	540, 558, 1568
FauI	CCCGC	1 cut(s)	344
FbaI	TGATCA	1 cut(s)	1453
FblI	GTMKAC	1 cut(s)	261
Fnu4HI	GCNGC	8 cut(s)	67, 427, 457, 460, 532, 611, 719, 1191
FokI	GGATG	4 cut(s)	88, 1005, 1388, 1412
FriOI	GRGCYC	3 cut(s)	309, 600, 1254
Fsp4HI	GCNGC	8 cut(s)	67, 427, 457, 460, 532, 611, 719, 1191
FspBI	CTAG	1 cut(s)	1404
GlaI	GCGC	5 cut(s)	443, 498, 550, 588, 1143
GluI	GCNGC	8 cut(s)	67, 427, 457, 460, 532, 611, 719, 1191
GsuI	CTGGAG	4 cut(s)	15, 857, 1079, 1479
HaeII	RGCGCY	3 cut(s)	552, 590, 1145
HaeIII	GGCC	6 cut(s)	150, 267, 302, 513, 718, 1286
HapII	CCGG	8 cut(s)	213, 220, 247, 274, 303, 463, 584, 1448
HgaI	GACGC	1 cut(s)	648
HhaI	GCGC	5 cut(s)	444, 499, 551, 589, 1144
Hin1I	GRCGYC	1 cut(s)	549
Hin1II	CATG	3 cut(s)	544, 562, 1572
Hin6I	GCGC	5 cut(s)	442, 497, 549, 587, 1142
HinP1I	GCGC	5 cut(s)	442, 497, 549, 587, 1142
HincII	GTYRAC	1 cut(s)	262
HindII	GTYRAC	1 cut(s)	262
HindIII	AAGCTT	3 cut(s)	521, 797, 977
HinfI	GANTC	7 cut(s)	26, 119, 258, 344, 421, 739, 1081
HpaII	CCGG	8 cut(s)	213, 220, 247, 274, 303, 463, 584, 1448
HphI	GGTGA	3 cut(s)	133, 862, 1394
Hpy166II	GTNNAC	2 cut(s)	262, 625
Hpy188I	TCNGA	8 cut(s)	124, 166, 482, 637, 1071, 1243, 1580, 1599
Hpy8I	GTNNAC	2 cut(s)	262, 625
Hpy99I	CGWCG	9 cut(s)	116, 179, 266, 401, 632, 664, 667, 670, 731
HpyAV	CCTTC	3 cut(s)	1013, 1344, 1575
HpyCH4III	ACNGT	6 cut(s)	9, 44, 506, 708, 787, 1218
HpyF3I	CTNAG	4 cut(s)	24, 254, 921, 971
Hsp92I	GRCGYC	1 cut(s)	549
Hsp92II	CATG	3 cut(s)	544, 562, 1572
HspAI	GCGC	5 cut(s)	442, 497, 549, 587, 1142
KasI	GGCGCC	1 cut(s)	548
KroI	GCCGGC	1 cut(s)	273
KroNI	GCCGGC	1 cut(s)	275
Ksp22I	TGATCA	1 cut(s)	1453
KspI	CCGCGG	2 cut(s)	457, 619
Kzo9I	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
LguI	GCTCTTC	1 cut(s)	369
Lsp1109I	GCAGC	3 cut(s)	413, 446, 1177
LweI	GCATC	5 cut(s)	359, 957, 1159, 1366, 1390
MaeI	CTAG	1 cut(s)	1404
MaeIII	GTNAC	5 cut(s)	139, 152, 349, 484, 850
MalI	GATC	9 cut(s)	168, 375, 386, 416, 644, 1159, 1357, 1393, 1455
MboI	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
MboII	GAAGA	6 cut(s)	128, 161, 322, 356, 529, 1222
MfeI	CAATTG	3 cut(s)	1107, 1117, 1161
MhlI	GDGCHC	7 cut(s)	42, 309, 579, 600, 1049, 1235, 1254
MlsI	TGGCCA	1 cut(s)	1286
MluNI	TGGCCA	1 cut(s)	1286
Mly113I	GGCGCC	1 cut(s)	549
MlyI	GAGTC	4 cut(s)	35, 113, 267, 338
Mox20I	TGGCCA	1 cut(s)	1286
MroNI	GCCGGC	1 cut(s)	273
MscI	TGGCCA	1 cut(s)	1286
MseI	TTAA	3 cut(s)	912, 1224, 1293
MslI	CAYNNNNRTG	1 cut(s)	987
Msp20I	TGGCCA	1 cut(s)	1286
MspA1I	CMGCKG	4 cut(s)	35, 456, 618, 765
MspI	CCGG	8 cut(s)	213, 220, 247, 274, 303, 463, 584, 1448
MspR9I	CCNGG	6 cut(s)	146, 299, 304, 392, 464, 584
MunI	CAATTG	3 cut(s)	1107, 1117, 1161
MvaI	CCWGG	3 cut(s)	146, 299, 392
MvnI	CGCG	5 cut(s)	442, 456, 491, 497, 618
NaeI	GCCGGC	1 cut(s)	275
NarI	GGCGCC	1 cut(s)	549
NciI	CCSGG	3 cut(s)	304, 464, 584
NdeII	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
NgoMIV	GCCGGC	1 cut(s)	273
NlaIII	CATG	3 cut(s)	544, 562, 1572
NmuCI	GTSAC	5 cut(s)	139, 152, 349, 484, 850
NspI	RCATGY	1 cut(s)	544
NspV	TTCGAA	1 cut(s)	878
PaeR7I	CTCGAG	1 cut(s)	590
PaqCI	CACCTGC	2 cut(s)	838, 1406
PasI	CCCWGGG	1 cut(s)	145
PciSI	GCTCTTC	1 cut(s)	369
PcsI	WCGNNNNNNNCGW	5 cut(s)	120, 339, 674, 875, 884
PdiI	GCCGGC	1 cut(s)	275
PfeI	GAWTC	3 cut(s)	421, 739, 1081
PflFI	GACNNNGTC	1 cut(s)	347
PflMI	CCANNNNNTGG	1 cut(s)	1129
PinAI	ACCGGT	1 cut(s)	246
PkrI	GCNGC	8 cut(s)	68, 428, 458, 461, 533, 612, 720, 1192
Ple19I	CGATCG	2 cut(s)	376, 645
PleI	GAGTC	4 cut(s)	34, 113, 266, 338
PluTI	GGCGCC	1 cut(s)	552
PpsI	GAGTC	4 cut(s)	34, 113, 266, 338
Psp124BI	GAGCTC	1 cut(s)	600
Psp6I	CCWGG	3 cut(s)	144, 297, 390
PspEI	GGTNACC	1 cut(s)	850
PspGI	CCWGG	3 cut(s)	144, 297, 390
PspPI	GGNCC	5 cut(s)	148, 409, 475, 511, 716
PspXI	VCTCGAGB	1 cut(s)	590
PstI	CTGCAG	1 cut(s)	431
PsyI	GACNNNGTC	1 cut(s)	347
PvuI	CGATCG	2 cut(s)	376, 645
RgaI	GCGATCGC	1 cut(s)	376
RsaI	GTAC	1 cut(s)	1428
RsaNI	GTAC	1 cut(s)	1427
RseI	CAYNNNNRTG	1 cut(s)	987
SacI	GAGCTC	1 cut(s)	600
SacII	CCGCGG	2 cut(s)	457, 619
SalI	GTCGAC	1 cut(s)	260
SapI	GCTCTTC	1 cut(s)	369
SaqAI	TTAA	3 cut(s)	912, 1224, 1293
SatI	GCNGC	8 cut(s)	67, 427, 457, 460, 532, 611, 719, 1191
Sau3AI	GATC	9 cut(s)	166, 373, 384, 414, 642, 1157, 1355, 1391, 1453
Sau96I	GGNCC	5 cut(s)	148, 409, 475, 511, 716
SchI	GAGTC	4 cut(s)	35, 113, 267, 338
ScrFI	CCNGG	6 cut(s)	146, 299, 304, 392, 464, 584
SduI	GDGCHC	7 cut(s)	42, 309, 579, 600, 1049, 1235, 1254
SfaAI	GCGATCGC	1 cut(s)	376
SfaNI	GCATC	5 cut(s)	359, 957, 1159, 1366, 1390
SfcI	CTRYAG	1 cut(s)	427
SfoI	GGCGCC	1 cut(s)	550
Sfr274I	CTCGAG	1 cut(s)	590
Sfr303I	CCGCGG	2 cut(s)	457, 619
SfuI	TTCGAA	1 cut(s)	878
SgfI	GCGATCGC	1 cut(s)	376
SgrAI	CRCCGGYG	1 cut(s)	246
SgrBI	CCGCGG	2 cut(s)	457, 619
SinI	GGWCC	2 cut(s)	409, 475
SlaI	CTCGAG	1 cut(s)	590
SmiMI	CAYNNNNRTG	1 cut(s)	987
SmlI	CTYRAG	4 cut(s)	446, 590, 685, 743
SmoI	CTYRAG	4 cut(s)	446, 590, 685, 743
SspDI	GGCGCC	1 cut(s)	548
SspMI	CTAG	1 cut(s)	1404
SstI	GAGCTC	1 cut(s)	600
StyD4I	CCNGG	6 cut(s)	144, 297, 302, 390, 462, 582
TaaI	ACNGT	6 cut(s)	9, 44, 506, 708, 787, 1218
TaqII	GACCGA	2 cut(s)	337, 869
TatI	WGTACW	1 cut(s)	1426
TauI	GCSGC	5 cut(s)	69, 459, 534, 613, 721
TfiI	GAWTC	3 cut(s)	421, 739, 1081
Tru1I	TTAA	3 cut(s)	912, 1224, 1293
Tru9I	TTAA	3 cut(s)	912, 1224, 1293
TscAI	CASTG	3 cut(s)	584, 713, 1615
TseFI	GTSAC	5 cut(s)	139, 152, 349, 484, 850
TseI	GCWGC	3 cut(s)	426, 459, 1190
Tsp45I	GTSAC	5 cut(s)	139, 152, 349, 484, 850
TspDTI	ATGAA	3 cut(s)	90, 575, 821
TspGWI	ACGGA	4 cut(s)	488, 592, 717, 1473
TspRI	CASTG	3 cut(s)	584, 713, 1615
Tth111I	GACNNNGTC	1 cut(s)	347
Van91I	CCANNNNNTGG	1 cut(s)	1129
VpaK11BI	GGWCC	2 cut(s)	409, 475
XapI	RAATTY	2 cut(s)	889, 931
XceI	RCATGY	1 cut(s)	544
XhoI	CTCGAG	1 cut(s)	590
XmiI	GTMKAC	1 cut(s)	261
XspI	CTAG	1 cut(s)	1404

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.