ATCG00440

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0020251
Physical Location & Seq
Reverse (-)
50001 .. 50363
363 bp
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UTR
Exon/CDS
Intron
ATCG00440.1

Sequence Viewer

Length: 363 bp
ATGTTTCTGCTTTACGAATATGATATTTTCTGGGCATTTTTACTAATATCAAGTGCTATTCCTGTTTTGGCATTTCTAATTTCCGGAGTTTTATCTCCAATTAGGAAGGGGCCGGAGAAACTTTCTAGTTATGAATCAGGTATAGAACCGATCGGGGATGCTTGGTTACAATTTAGAATCCGTTATTATATGTTTGCTCTAGTTTTTGTTGTTTTTGATGTTGAAACTGTTTTTCTGTATCCGTGGGCAATGAGTTTCGATGTACTGGGGGTATCTGCTTTTATAGAAGCTTTCATTTTCGTGCTTATCCTAATTCTTGGTTTAGTTTATGCATGGCGAAAAGGAGCATTGGAATGGTCTTAG

Protein Analysis

120

Amino Acids

13.84

Weight (kDa)

4.58

Isoelectric Point (pI)

41.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q4 PF00507 23 - 119 2.8e-37 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020251)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00440
malus_domestica MD03G1163700.v1.1 MD11G1295600.v1.1
rosa_chinensis RchiOBHm_CPg0502161
rosa_roxburghii Rroxscaffold_1G00030900
rosa_wichuraiana Rw7G039190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 83
AfaI GTAC 1 cut(s) 264
AgsI TTSAA 1 cut(s) 224
AluBI AGCT 1 cut(s) 290
AluI AGCT 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 83
AoxI GGCC 1 cut(s) 110
AspS9I GGNCC 1 cut(s) 110
BciVI GTATCC 1 cut(s) 249
BfaI CTAG 2 cut(s) 126, 200
BfuI GTATCC 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 110
BmiI GGNNCC 1 cut(s) 111
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 1 cut(s) 148
BmuI ACTGGG 1 cut(s) 275
BsaJI CCNNGG 1 cut(s) 242
BsaWI WCCGGW 1 cut(s) 83
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 1 cut(s) 255
BseAI TCCGGA 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 242
BseGI GGATG 1 cut(s) 163
BseMI GCAATG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 270
Bsh1285I CGRYCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 112
BsiEI CGRYCG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 84, 113
BsnI GGCC 1 cut(s) 112
Bsp13I TCCGGA 1 cut(s) 83
Bsp143I GATC 1 cut(s) 150
BspANI GGCC 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 83
BspLI GGNNCC 1 cut(s) 111
BsrDI GCAATG 1 cut(s) 255
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 1 cut(s) 242
BssMI GATC 1 cut(s) 150
Bst4CI ACNGT 1 cut(s) 229
BstDEI CTNAG 1 cut(s) 360
BstDSI CCRYGG 1 cut(s) 242
BstF5I GGATG 1 cut(s) 163
BstKTI GATC 1 cut(s) 153
BstMBI GATC 1 cut(s) 150
BstMCI CGRYCG 1 cut(s) 153
BsuI GTATCC 1 cut(s) 249
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 242
BtsCI GGATG 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 263
CviAII CATG 1 cut(s) 333
CviJI RGCY 2 cut(s) 112, 290
CviKI_1 RGCY 2 cut(s) 112, 290
CviQI GTAC 1 cut(s) 263
DdeI CTNAG 1 cut(s) 360
DpnI GATC 1 cut(s) 152
DpnII GATC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 334
FaeI CATG 1 cut(s) 336
FaiI YATR 8 cut(s) 21, 132, 143, 189, 191, 284, 330, 334
FatI CATG 1 cut(s) 332
FokI GGATG 1 cut(s) 170
FspBI CTAG 2 cut(s) 126, 200
HaeIII GGCC 1 cut(s) 112
HapII CCGG 2 cut(s) 84, 113
Hin1II CATG 1 cut(s) 336
HindIII AAGCTT 1 cut(s) 288
HinfI GANTC 2 cut(s) 134, 177
HpaII CCGG 2 cut(s) 84, 113
Hpy188III TCNNGA 1 cut(s) 84
HpyAV CCTTC 1 cut(s) 100
HpyCH4III ACNGT 1 cut(s) 229
HpyCH4V TGCA 1 cut(s) 332
HpyF3I CTNAG 1 cut(s) 360
Hsp92II CATG 1 cut(s) 336
Kpn2I TCCGGA 1 cut(s) 83
Kzo9I GATC 1 cut(s) 150
LmnI GCTCC 1 cut(s) 344
LpnPI CCDG 6 cut(s) 16, 75, 97, 123, 126, 251
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 126, 200
MaeIII GTNAC 1 cut(s) 165
MalI GATC 1 cut(s) 152
MboI GATC 1 cut(s) 150
MluCI AATT 4 cut(s) 78, 99, 170, 312
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 83
MslI CAYNNNNRTG 2 cut(s) 299, 352
MspI CCGG 2 cut(s) 84, 113
NdeII GATC 1 cut(s) 150
NlaIII CATG 1 cut(s) 336
NlaIV GGNNCC 1 cut(s) 111
NsiI ATGCAT 1 cut(s) 334
PfeI GAWTC 2 cut(s) 134, 177
Ple19I CGATCG 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 111
PspPI GGNCC 1 cut(s) 110
PvuI CGATCG 1 cut(s) 153
RsaI GTAC 1 cut(s) 264
RsaNI GTAC 1 cut(s) 263
RseI CAYNNNNRTG 2 cut(s) 299, 352
Sau3AI GATC 1 cut(s) 150
Sau96I GGNCC 1 cut(s) 110
SetI ASST 2 cut(s) 142, 292
SfaNI GCATC 1 cut(s) 148
SmiMI CAYNNNNRTG 2 cut(s) 299, 352
Sse9I AATT 4 cut(s) 78, 99, 170, 312
SspMI CTAG 2 cut(s) 126, 200
TaaI ACNGT 1 cut(s) 229
TaqI TCGA 1 cut(s) 258
TasI AATT 4 cut(s) 78, 99, 170, 312
TatI WGTACW 1 cut(s) 262
TfiI GAWTC 2 cut(s) 134, 177
TspDTI ATGAA 2 cut(s) 147, 283
TspGWI ACGGA 2 cut(s) 170, 231
XspI CTAG 2 cut(s) 126, 200
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.