RCHIOBHM_CPG0502161

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Pt
OG0020251
Physical Location & Seq
Forward (+)
60724 .. 61199
476 bp
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UTR
Exon/CDS
Intron
PRQ15703

Sequence Viewer

Length: 438 bp
ATGGCTGATTTGACAATTTATTCTATTTTGAATCGACTAATCCGGTATATTTTCCACATTAATAGGAGTACGTCTATGCTTCTGCTTTACGAATATGATTTTTTCTGGGCATTTCTGATAGTTTCAAGTGTTATTCCTATTTTGGCATTTTTCATTTCCGGAGTTTTAGCCCCGATTAGCAAAGGGCCAGAAAAACTTTCGAGTTATGAATCGGGAATAGAACCAATGGGGGATGCTTGGTTACAATTTCGAATCCGTTATTATATGTTTGCTCTAGTTTTTGTTGTTTTTGATGTTGAAACGGTTTTTCTTTATCCATGGGCAATGAGTTTCGATGTATTGGGAGTATCCGTATTTATAGAAGCTTTAATTTTCGTGCTTATCCTAATTGTTGGTTCAATTTATGCATGGCGAAAAGGAGCATTGGAATGGTCTTAG

Protein Analysis

145

Amino Acids

16.8

Weight (kDa)

4.99

Isoelectric Point (pI)

38.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Oxidored_q4 PF00507 48 - 144 8.4e-36 NADH-ubiquinone/plastoquinone oxidoreductase, chain 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020251)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG00440
malus_domestica MD03G1163700.v1.1 MD11G1295600.v1.1
rosa_chinensis RchiOBHm_CPg0502161
rosa_roxburghii Rroxscaffold_1G00030900
rosa_wichuraiana Rw7G039190

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 158
AfaI GTAC 1 cut(s) 70
AgsI TTSAA 4 cut(s) 31, 126, 299, 399
AluBI AGCT 1 cut(s) 365
AluI AGCT 1 cut(s) 365
Aor13HI TCCGGA 1 cut(s) 158
AoxI GGCC 1 cut(s) 185
AseI ATTAAT 1 cut(s) 60
AspS9I GGNCC 1 cut(s) 185
AsuII TTCGAA 1 cut(s) 250
BciVI GTATCC 1 cut(s) 358
BfaI CTAG 1 cut(s) 275
BfuI GTATCC 1 cut(s) 358
BmgT120I GGNCC 1 cut(s) 185
BmsI GCATC 1 cut(s) 223
Bpu14I TTCGAA 1 cut(s) 250
BsaJI CCNNGG 1 cut(s) 317
BsaWI WCCGGW 2 cut(s) 42, 158
BsaXI ACNNNNNCTCC 2 cut(s) 336, 366
Bse3DI GCAATG 1 cut(s) 330
BseAI TCCGGA 1 cut(s) 158
BseDI CCNNGG 1 cut(s) 317
BseGI GGATG 1 cut(s) 238
BseMI GCAATG 1 cut(s) 330
BshFI GGCC 1 cut(s) 187
BsiSI CCGG 2 cut(s) 43, 159
BsnI GGCC 1 cut(s) 187
Bsp119I TTCGAA 1 cut(s) 250
Bsp13I TCCGGA 1 cut(s) 158
Bsp19I CCATGG 1 cut(s) 317
BspANI GGCC 1 cut(s) 187
BspEI TCCGGA 1 cut(s) 158
BspT104I TTCGAA 1 cut(s) 250
BsrDI GCAATG 1 cut(s) 330
BssECI CCNNGG 1 cut(s) 317
BssT1I CCWWGG 1 cut(s) 317
Bst4CI ACNGT 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 250
BstDEI CTNAG 1 cut(s) 435
BstDSI CCRYGG 1 cut(s) 317
BstF5I GGATG 1 cut(s) 238
BsuI GTATCC 1 cut(s) 358
BsuRI GGCC 1 cut(s) 187
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 238
Cfr13I GGNCC 1 cut(s) 185
Csp6I GTAC 1 cut(s) 69
CviAII CATG 2 cut(s) 318, 408
CviJI RGCY 4 cut(s) 5, 170, 187, 365
CviKI_1 RGCY 4 cut(s) 5, 170, 187, 365
CviQI GTAC 1 cut(s) 69
DdeI CTNAG 1 cut(s) 435
Eco130I CCWWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 317
EcoT22I ATGCAT 1 cut(s) 409
ErhI CCWWGG 1 cut(s) 317
FaeI CATG 2 cut(s) 321, 411
FatI CATG 2 cut(s) 317, 407
FokI GGATG 1 cut(s) 245
FspBI CTAG 1 cut(s) 275
HaeIII GGCC 1 cut(s) 187
HapII CCGG 2 cut(s) 43, 159
Hin1II CATG 2 cut(s) 321, 411
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 3 cut(s) 31, 209, 252
HpaII CCGG 2 cut(s) 43, 159
Hpy188I TCNGA 1 cut(s) 117
Hpy188III TCNNGA 2 cut(s) 159, 213
HpyCH4III ACNGT 1 cut(s) 304
HpyCH4IV ACGT 1 cut(s) 71
HpyCH4V TGCA 1 cut(s) 407
HpyF3I CTNAG 1 cut(s) 435
HpySE526I ACGT 1 cut(s) 71
Hsp92II CATG 2 cut(s) 321, 411
Kpn2I TCCGGA 1 cut(s) 158
LmnI GCTCC 1 cut(s) 419
LpnPI CCDG 4 cut(s) 56, 91, 172, 201
LweI GCATC 1 cut(s) 223
MaeI CTAG 1 cut(s) 275
MaeII ACGT 1 cut(s) 71
MaeIII GTNAC 1 cut(s) 240
MluCI AATT 5 cut(s) 15, 245, 369, 387, 399
Mph1103I ATGCAT 1 cut(s) 409
MroI TCCGGA 1 cut(s) 158
MseI TTAA 2 cut(s) 60, 368
MslI CAYNNNNRTG 1 cut(s) 427
MspI CCGG 2 cut(s) 43, 159
NcoI CCATGG 1 cut(s) 317
NlaIII CATG 2 cut(s) 321, 411
NsiI ATGCAT 1 cut(s) 409
NspV TTCGAA 1 cut(s) 250
PfeI GAWTC 3 cut(s) 31, 209, 252
PshBI ATTAAT 1 cut(s) 60
PspPI GGNCC 1 cut(s) 185
RsaI GTAC 1 cut(s) 70
RsaNI GTAC 1 cut(s) 69
RseI CAYNNNNRTG 1 cut(s) 427
SaqAI TTAA 2 cut(s) 60, 368
Sau96I GGNCC 1 cut(s) 185
SetI ASST 2 cut(s) 74, 367
SfaNI GCATC 1 cut(s) 223
SfuI TTCGAA 1 cut(s) 250
SmiMI CAYNNNNRTG 1 cut(s) 427
Sse9I AATT 5 cut(s) 15, 245, 369, 387, 399
SspMI CTAG 1 cut(s) 275
StyI CCWWGG 1 cut(s) 317
TaaI ACNGT 1 cut(s) 304
TaiI ACGT 1 cut(s) 74
TaqI TCGA 4 cut(s) 34, 200, 250, 333
TasI AATT 5 cut(s) 15, 245, 369, 387, 399
TfiI GAWTC 3 cut(s) 31, 209, 252
Tru1I TTAA 2 cut(s) 60, 368
Tru9I TTAA 2 cut(s) 60, 368
TspDTI ATGAA 2 cut(s) 142, 222
TspGWI ACGGA 2 cut(s) 245, 340
VspI ATTAAT 1 cut(s) 60
XspI CTAG 1 cut(s) 275
Zsp2I ATGCAT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.