ATCG01010

NDH shuttles electrons from NAD(P)H plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
Pt
OG0020165
Physical Location & Seq
Reverse (-)
110398 .. 112638
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
ATCG01010.1

Sequence Viewer

Length: 2241 bp
ATGGAACATACATATCAATATTCATGGATCATCCCTTTCATTCCACTTCCAGTACCTATTTTACTAGGAGTTGGACTTCTCCTTTTTCCGACAGCAACAAAAAACCTTCGCCGCATGTGGACTTTTCTTAGTATTTTTTTGTTAAGTATAGTTATGATCTTTTCAATCTATCTATCTATTCAACAAATTTTTCTAAGTTGCATTCATCAAAATGTATGGTCTTGGACCATAAATAATGAATTTTCTTTTGAGTTCGGTTACTTTATCGATCCACTTACTTCTATTATGTCAATATTAATTACAACTGTTGGGATTTTGGTTCTGATTTATAGTGACAATTATATGTCGCATGATCAAGGATACCTGAGATTTTTTGCTTATATGGGTTTTTTTAATACTTCAATGTTAGGATTAGTTACTAGTTCTAATTTGATCCAAGTTTATTTTTTTTGGGAATTAGTTGGAATGTGTTCGTATTTATTAATAGGTTTTTGGTTCACACGACCTATTGCAGCGAATGCTTGTCAAAAAGCTTTTGTAACTAATCGTGTAGGGGATTTTGGTTTATTATTAGGAATTTTAGGTCTTTATTGGATAACTGGCAGTTTTGAATTTCAGGATTTGTTCGAAATATTCAATAATTTAATTTTAAATAATAGAGTAAATCTCTTATTCCTTACTTTGTGTGCATTTCTATTATTTGTAGGTCCTATTGCTAAATCCGCACAATTTCCTCTTCATGTATGGTTACCTGATGCCATGGAGGGCCCTACTCCCATTTCGGCTCTTATACATGCTGCTACTATGGTAGCGGCGGGTATTTTTCTTGTAGCTCGTCTTCTTCCTCTTTTTATCGTTATCCCTTCTATAATGTATATAATATCTTTGATAGGTATAATAACAGTACTCTTAGGAGCCACTTTAGCTCTTGCTCAAAAAGACATTAAGCGGGGTTTAGCCTATTCTACAATGTCTCAACTCGGTTATATGATGTTAGCTCTAGGTATGGGGTCTTATCGATCTGCTTTATTTCATTTGATTACTCATGCTTATTCGAAAGCTTTGTTGTTTTTAGGATCTGGATCCATTATTCATTCAATGGAAGCTATAGTTGGATATTCTCCCGATAAAAGTCAGAATATGATTCTTATGGGTGGTTTGACAAAACATGTCCCGATTACAAAAACGGCCTTTTTAATAGGAACACTCTCTCTTTGTGGTATCCCCCCCCTTGCTTGTTTTTGGTCGAAAGATGAAATTCTTAATGATAGTTTGTTATTTTCGCCAATTTTTGCAATAATAGCTTGTTCAACAGCGGGATTAACCGCATTTTATATGTTTCGGATTTATTTACTTACTTTTGAAGGACATTTAAACACTTATTTTCTAAATTACAGTGGAAAAAAAAGTGGCTCCTTCTATTCACTCTCTTTATGGGGTAAAGAAGAAGAAAAAAAACTTAATAAAAATTTTGGGTTAGTACCATTATTAACAATGAATAATACAAAAAGAGCTTCTTTTTTTTGCAATAAAACATATAAAATAAGTAATAATGTAAGAAATCAAATTTTTATTACTGTTGAAAATTTTGGACTTAATACAAGAACTTTCTATTATCCGCATGAATCAGACAATACTATTCTATTTCCTATGCTTATTTTGGTTTTATTTACTTTGTTTATTGGAGCCATAGGAATTCCTTTCAATCAAGAAGGAATAGACTTTGATATATTATCAAAATTTTTCACGCCGTCAATAAACCTTTTGCATAAAAATTCACAAAATTTTGTAGATTGGTATGAATTTTTGAGAAATGCAACTTTTTCGGTCAGTATAGCCTTTTTTGGAATATTTATAGCATACTGTTTATATAAGCCTTTTTATTCATCTTTATTAAATTTAACCTTACTTAATTCATTTCAAAAATGGAATTCTAAACGAATTCATTGGGAAAAACTAATAAATTTTGTATATAATTGGTCATATAATCGTGGTTACATAGATTCTTTTTTTAAAACATCTTTAATTGAAAGTATAAGAAGATTAGCAAAACAAACGACTTTTTTTGATAAACGAATCATTGATGGAATTACAAATGGAGTAGGTATTACAAGTTTCTTTGTAGGAGAAGTAACAAAATATATAGGTGGAAGTCGCATCTCTTCTTATCTGTTCTTATATTTGTCCTATGTATTGATTTTTTTAATGATCCTCTTTTTTTTTTATTTTGAAAAATTCTAA

Protein Analysis

746

Amino Acids

85.24

Weight (kDa)

9.02

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_N PF00662 75 - 125 3.2e-16 NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus
Proton_antipo_M PF00361 141 - 440 7.5e-93 NADH:quinone oxidoreductase/Mrp antiporter, TM
Proton_antipo_C PF01010 448 - 690 6.8e-110 NADH-dehyrogenase subunit F, TMs, (complex I) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020165)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01010
fragaria_vesca FvH4_c3g00021
malus_domestica MD00G1121700.v1.1
prunus_persica Prupe.2G145900_v2.0.a1
rosa_chinensis RchiOBHm_CPg0501681
rosa_laevigata RLG00000000210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 8 cut(s) 112, 723, 812, 815, 949, 1316, 1326, 1619
AclWI GGATC 7 cut(s) 35, 263, 427, 1077, 1084, 1090, 2203
AfaI GTAC 3 cut(s) 54, 906, 1482
AflIII ACRYGT 1 cut(s) 1168
AhlI ACTAGT 1 cut(s) 419
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
AluBI AGCT 8 cut(s) 533, 833, 926, 998, 1061, 1106, 1304, 1514
AluI AGCT 8 cut(s) 533, 833, 926, 998, 1061, 1106, 1304, 1514
Alw26I GTCTC 1 cut(s) 978
AlwI GGATC 7 cut(s) 35, 263, 427, 1077, 1084, 1090, 2203
AoxI GGCC 2 cut(s) 766, 1188
ApaI GGGCCC 1 cut(s) 770
ApeKI GCWGC 2 cut(s) 512, 797
AseI ATTAAT 2 cut(s) 296, 482
Asp700I GAANNNNTTC 1 cut(s) 469
AspS9I GGNCC 4 cut(s) 225, 707, 766, 767
AsuII TTCGAA 2 cut(s) 627, 1055
AvaII GGWCC 2 cut(s) 225, 707
BaeGI GKGCMC 1 cut(s) 770
BamHI GGATCC 1 cut(s) 1082
BanII GRGCYC 1 cut(s) 770
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BbsI GAAGAC 1 cut(s) 830
BbvI GCAGC 2 cut(s) 524, 784
BccI CCATC 1 cut(s) 2078
BceAI ACGGC 2 cut(s) 1203, 1735
BcgI CGANNNNNNTGC 2 cut(s) 1806, 1840
BciVI GTATCC 2 cut(s) 353, 1232
BclI TGATCA 1 cut(s) 352
BcoDI GTCTC 1 cut(s) 978
BcuI ACTAGT 1 cut(s) 419
BfaI CTAG 3 cut(s) 65, 420, 1001
BfmI CTRYAG 1 cut(s) 1107
BfuI GTATCC 2 cut(s) 353, 1232
BisI GCNGC 4 cut(s) 112, 513, 798, 813
BlsI GCNGC 4 cut(s) 113, 514, 799, 814
BmcAI AGTACT 1 cut(s) 906
Bme18I GGWCC 2 cut(s) 225, 707
BmgT120I GGNCC 4 cut(s) 225, 707, 766, 767
BmiI GGNNCC 5 cut(s) 768, 916, 1084, 1414, 1687
BmsI GCATC 2 cut(s) 745, 2166
BpiI GAAGAC 1 cut(s) 830
BplI GAGNNNNNCTC 2 cut(s) 651, 683
Bpu14I TTCGAA 2 cut(s) 627, 1055
Bsa29I ATCGAT 2 cut(s) 267, 1018
BsaBI GATNNNNATC 1 cut(s) 1081
BsaJI CCNNGG 1 cut(s) 759
Bse1I ACTGG 2 cut(s) 50, 604
Bse8I GATNNNNATC 1 cut(s) 1081
BseCI ATCGAT 2 cut(s) 267, 1018
BseDI CCNNGG 1 cut(s) 759
BseGI GGATG 1 cut(s) 30
BseJI GATNNNNATC 1 cut(s) 1081
BseMII CTCAG 1 cut(s) 356
BseNI ACTGG 2 cut(s) 50, 604
BseSI GKGCMC 1 cut(s) 770
BseXI GCAGC 2 cut(s) 524, 784
BshFI GGCC 2 cut(s) 768, 1190
BshVI ATCGAT 2 cut(s) 267, 1018
BslFI GGGAC 1 cut(s) 1157
BsmAI GTCTC 1 cut(s) 978
BsmFI GGGAC 1 cut(s) 1157
BsmI GAATGC 2 cut(s) 201, 523
BsnI GGCC 2 cut(s) 768, 1190
Bsp119I TTCGAA 2 cut(s) 627, 1055
Bsp120I GGGCCC 1 cut(s) 766
Bsp1286I GDGCHC 1 cut(s) 770
Bsp143I GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
Bsp19I CCATGG 1 cut(s) 759
BspACI CCGC 8 cut(s) 112, 723, 812, 815, 949, 1316, 1326, 1619
BspANI GGCC 2 cut(s) 768, 1190
BspCNI CTCAG 1 cut(s) 357
BspDI ATCGAT 2 cut(s) 267, 1018
BspLI GGNNCC 5 cut(s) 768, 916, 1084, 1414, 1687
BspPI GGATC 7 cut(s) 35, 263, 427, 1077, 1084, 1090, 2203
BspT104I TTCGAA 2 cut(s) 627, 1055
BsrI ACTGG 2 cut(s) 50, 604
BssECI CCNNGG 1 cut(s) 759
BssMI GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
BssT1I CCWWGG 1 cut(s) 759
Bst4CI ACNGT 5 cut(s) 307, 904, 1397, 1579, 1865
Bst6I CTCTTC 2 cut(s) 741, 2167
BstAPI GCANNNNNTGC 1 cut(s) 518
BstBI TTCGAA 2 cut(s) 627, 1055
BstDEI CTNAG 4 cut(s) 128, 194, 365, 910
BstDSI CCRYGG 1 cut(s) 759
BstEII GGTNACC 1 cut(s) 747
BstF5I GGATG 1 cut(s) 30
BstKTI GATC 9 cut(s) 30, 159, 271, 355, 435, 1022, 1079, 1085, 2211
BstMAI GTCTC 1 cut(s) 978
BstMBI GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
BstMWI GCNNNNNNNGC 4 cut(s) 518, 722, 923, 1301
BstNSI RCATGY 3 cut(s) 118, 797, 1172
BstPI GGTNACC 1 cut(s) 747
BstSFI CTRYAG 1 cut(s) 1107
BstSLI GKGCMC 1 cut(s) 770
BstV1I GCAGC 2 cut(s) 524, 784
BstV2I GAAGAC 1 cut(s) 830
BstX2I RGATCY 2 cut(s) 1076, 1082
BstYI RGATCY 2 cut(s) 1076, 1082
Bsu15I ATCGAT 2 cut(s) 267, 1018
BsuI GTATCC 2 cut(s) 353, 1232
BsuRI GGCC 2 cut(s) 768, 1190
BsuTUI ATCGAT 2 cut(s) 267, 1018
BtgI CCRYGG 1 cut(s) 759
BtsCI GGATG 1 cut(s) 30
BtsIMutI CAGTG 1 cut(s) 1402
Cfr13I GGNCC 4 cut(s) 225, 707, 766, 767
ClaI ATCGAT 2 cut(s) 267, 1018
Csp6I GTAC 3 cut(s) 53, 905, 1481
CviAII CATG 9 cut(s) 24, 115, 350, 740, 760, 794, 1046, 1169, 1622
CviQI GTAC 3 cut(s) 53, 905, 1481
DdeI CTNAG 4 cut(s) 128, 194, 365, 910
DpnI GATC 9 cut(s) 29, 158, 270, 354, 434, 1021, 1078, 1084, 2210
DpnII GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
DraI TTTAAA 3 cut(s) 651, 1374, 2014
Eam1104I CTCTTC 2 cut(s) 741, 2167
EarI CTCTTC 2 cut(s) 741, 2167
Eco130I CCWWGG 1 cut(s) 759
Eco24I GRGCYC 1 cut(s) 770
Eco47I GGWCC 2 cut(s) 225, 707
Eco91I GGTNACC 1 cut(s) 747
EcoO109I RGGNCCY 3 cut(s) 707, 766, 767
EcoO65I GGTNACC 1 cut(s) 747
EcoRI GAATTC 3 cut(s) 1695, 1930, 1941
EcoT14I CCWWGG 1 cut(s) 759
EcoT38I GRGCYC 1 cut(s) 770
ErhI CCWWGG 1 cut(s) 759
FaeI CATG 9 cut(s) 27, 118, 353, 743, 763, 797, 1049, 1172, 1625
FalI AAGNNNNNCTT 2 cut(s) 1501, 1533
FaqI GGGAC 1 cut(s) 1157
FatI CATG 9 cut(s) 23, 114, 349, 739, 759, 793, 1045, 1168, 1621
FauI CCCGC 3 cut(s) 808, 942, 1309
FbaI TGATCA 1 cut(s) 352
Fnu4HI GCNGC 4 cut(s) 112, 513, 798, 813
FokI GGATG 1 cut(s) 17
FriOI GRGCYC 1 cut(s) 770
Fsp4HI GCNGC 4 cut(s) 112, 513, 798, 813
FspBI CTAG 3 cut(s) 65, 420, 1001
GluI GCNGC 4 cut(s) 112, 513, 798, 813
HaeIII GGCC 2 cut(s) 768, 1190
Hin1II CATG 9 cut(s) 27, 118, 353, 743, 763, 797, 1049, 1172, 1625
HindIII AAGCTT 2 cut(s) 531, 1059
HinfI GANTC 4 cut(s) 1144, 1625, 2003, 2076
Hpy166II GTNNAC 2 cut(s) 120, 498
Hpy188I TCNGA 5 cut(s) 90, 324, 1137, 1344, 1630
Hpy188III TCNNGA 5 cut(s) 617, 1080, 1124, 1174, 1709
Hpy8I GTNNAC 2 cut(s) 120, 498
HpyAV CCTTC 5 cut(s) 116, 873, 1358, 1426, 1706
HpyCH4III ACNGT 5 cut(s) 307, 904, 1397, 1579, 1865
HpyCH4V TGCA 7 cut(s) 201, 512, 689, 1295, 1527, 1768, 1817
HpyF10VI GCNNNNNNNGC 4 cut(s) 518, 722, 923, 1301
HpyF3I CTNAG 4 cut(s) 128, 194, 365, 910
Hsp92II CATG 9 cut(s) 27, 118, 353, 743, 763, 797, 1049, 1172, 1625
Ksp22I TGATCA 1 cut(s) 352
Kzo9I GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
LmnI GCTCC 3 cut(s) 914, 1418, 1685
LpnPI CCDG 6 cut(s) 63, 377, 585, 602, 765, 1065
Lsp1109I GCAGC 2 cut(s) 524, 784
LweI GCATC 2 cut(s) 745, 2166
MaeI CTAG 3 cut(s) 65, 420, 1001
MaeIII GTNAC 7 cut(s) 257, 332, 415, 538, 747, 1994, 2131
MalI GATC 9 cut(s) 29, 158, 270, 354, 434, 1021, 1078, 1084, 2210
MboI GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
MboII GAAGA 7 cut(s) 728, 830, 833, 1457, 1460, 2052, 2154
MflI RGATCY 2 cut(s) 1076, 1082
MhlI GDGCHC 1 cut(s) 770
MmeI TCCRAC 4 cut(s) 52, 113, 442, 1093
MnlI CCTC 4 cut(s) 744, 757, 855, 2222
MroXI GAANNNNTTC 1 cut(s) 469
MslI CAYNNNNRTG 1 cut(s) 210
MspA1I CMGCKG 1 cut(s) 1316
Mva1269I GAATGC 2 cut(s) 201, 523
MwoI GCNNNNNNNGC 4 cut(s) 518, 722, 923, 1301
NcoI CCATGG 1 cut(s) 759
NdeII GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
NlaIII CATG 9 cut(s) 27, 118, 353, 743, 763, 797, 1049, 1172, 1625
NlaIV GGNNCC 5 cut(s) 768, 916, 1084, 1414, 1687
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 3 cut(s) 118, 797, 1172
NspV TTCGAA 2 cut(s) 627, 1055
PciI ACATGT 1 cut(s) 1168
PctI GAATGC 2 cut(s) 201, 523
PdmI GAANNNNTTC 1 cut(s) 469
PfeI GAWTC 4 cut(s) 1144, 1625, 2003, 2076
PkrI GCNGC 4 cut(s) 113, 514, 799, 814
PpuMI RGGWCCY 1 cut(s) 707
PscI ACATGT 1 cut(s) 1168
PshBI ATTAAT 2 cut(s) 296, 482
Psp5II RGGWCCY 1 cut(s) 707
PspEI GGTNACC 1 cut(s) 747
PspN4I GGNNCC 5 cut(s) 768, 916, 1084, 1414, 1687
PspOMI GGGCCC 1 cut(s) 766
PspPI GGNCC 4 cut(s) 225, 707, 766, 767
PspPPI RGGWCCY 1 cut(s) 707
PsuI RGATCY 2 cut(s) 1076, 1082
RsaI GTAC 3 cut(s) 54, 906, 1482
RsaNI GTAC 3 cut(s) 53, 905, 1481
RseI CAYNNNNRTG 1 cut(s) 210
SatI GCNGC 4 cut(s) 112, 513, 798, 813
Sau3AI GATC 9 cut(s) 27, 156, 268, 352, 432, 1019, 1076, 1082, 2208
Sau96I GGNCC 4 cut(s) 225, 707, 766, 767
ScaI AGTACT 1 cut(s) 906
SduI GDGCHC 1 cut(s) 770
SfaNI GCATC 2 cut(s) 745, 2166
SfcI CTRYAG 1 cut(s) 1107
SfuI TTCGAA 2 cut(s) 627, 1055
SinI GGWCC 2 cut(s) 225, 707
SmiMI CAYNNNNRTG 1 cut(s) 210
SpeI ACTAGT 1 cut(s) 419
SsiI CCGC 8 cut(s) 112, 723, 812, 815, 949, 1316, 1326, 1619
SspI AATATT 4 cut(s) 20, 294, 633, 1851
SspMI CTAG 3 cut(s) 65, 420, 1001
StyI CCWWGG 1 cut(s) 759
TaaI ACNGT 5 cut(s) 307, 904, 1397, 1579, 1865
TaqI TCGA 5 cut(s) 267, 627, 1018, 1055, 1247
TaqII GACCGA 1 cut(s) 1816
TatI WGTACW 1 cut(s) 904
TauI GCSGC 2 cut(s) 114, 815
TfiI GAWTC 4 cut(s) 1144, 1625, 2003, 2076
TscAI CASTG 1 cut(s) 1402
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 512, 797
Tsp45I GTSAC 1 cut(s) 332
TspRI CASTG 1 cut(s) 1402
VpaK11BI GGWCC 2 cut(s) 225, 707
VspI ATTAAT 2 cut(s) 296, 482
XceI RCATGY 3 cut(s) 118, 797, 1172
XmnI GAANNNNTTC 1 cut(s) 469
XspI CTAG 3 cut(s) 65, 420, 1001
ZrmI AGTACT 1 cut(s) 906
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.