RLG00000000210

A subunit of NADH dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0020165
Physical Location & Seq
Forward (+)
17183 .. 18624
1442 bp
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UTR
Exon/CDS
Intron
RLM00000000210

Sequence Viewer

Length: 648 bp
ATGGAATATACATATCAATATTCATGGATCATACCGTTCATTCCACTTCCAGTTCCTATGTTAATAGGAGCTGGACTTCTACTTTTTCCAACGGCAACTAAAAATCTTCGCCGTATGTGGGCTTTTCCGAGTTTTTGGTTCACCCGACCTAGTGCGGCGAATGCTTGTCAAAAAGCGTTTGTAACTAATCGTGTCGGGGATTTTGGGTTATTATTAGGAATTTTAGGTTTTTATTGGATAACAGGTAGTTTTGAATTTCGGGATTTGTTTGAAATATTCAATAACTTGGTTTCCAATAATGAAGTTAATCCTTTATTTGTTACTTTATGTGCCTTCCTATTATTTGCCGGCGCAGTTGCGAAATCTGCTCAATTTCCCCTTCATGTCTGGTTACCCGATGCCATGGAAGGGCCTACCCCTATTTCGGCTCTTATACATGCTGCTACGATGGTAGCAGCAGGAATTTTTCTTGTAGCTCGGCTTCTTCCTCTTTTCATAGCTATACCTTACATATTAAATCTAATATCTTTGATCGGATCTGGATCTATTATTCATTCGATGGAAGCTATTGTTGGGTATTCTCCAGATAAAAGTCAGAATATGTGGCAAAAAAAGCAGCTCATTCTATTCAATGTCTCTGTGGGGTAA

Protein Analysis

216

Amino Acids

23.9

Weight (kDa)

8.6

Isoelectric Point (pI)

26.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_M PF00361 45 - 184 1.9e-36 NADH:quinone oxidoreductase/Mrp antiporter, TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0020165)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01010
fragaria_vesca FvH4_c3g00021
malus_domestica MD00G1121700.v1.1
prunus_persica Prupe.2G145900_v2.0.a1
rosa_chinensis RchiOBHm_CPg0501681
rosa_laevigata RLG00000000210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 155
AclWI GGATC 3 cut(s) 35, 544, 550
AcsI RAATTY 3 cut(s) 219, 254, 462
AfiI CCNNNNNNNGG 3 cut(s) 118, 408, 424
AgsI TTSAA 4 cut(s) 254, 272, 280, 631
AjuI GAANNNNNNNTTGG 2 cut(s) 555, 587
AluBI AGCT 5 cut(s) 71, 476, 500, 566, 619
AluI AGCT 5 cut(s) 71, 476, 500, 566, 619
Alw26I GTCTC 1 cut(s) 640
AlwI GGATC 3 cut(s) 35, 544, 550
AoxI GGCC 1 cut(s) 410
ApeKI GCWGC 3 cut(s) 440, 455, 616
ApoI RAATTY 3 cut(s) 219, 254, 462
AspLEI GCGC 1 cut(s) 353
AspS9I GGNCC 1 cut(s) 410
AsuHPI GGTGA 1 cut(s) 133
BarI GAAGNNNNNNTAC 2 cut(s) 465, 497
BbvI GCAGC 3 cut(s) 427, 467, 628
BccI CCATC 2 cut(s) 442, 553
BceAI ACGGC 2 cut(s) 96, 108
BcoDI GTCTC 1 cut(s) 640
BfaI CTAG 1 cut(s) 150
BisI GCNGC 4 cut(s) 156, 441, 456, 617
BlsI GCNGC 4 cut(s) 157, 442, 457, 618
BmgT120I GGNCC 1 cut(s) 410
BmsI GCATC 1 cut(s) 388
BpmI CTGGAG 1 cut(s) 567
BsaBI GATNNNNATC 1 cut(s) 541
BsaJI CCNNGG 1 cut(s) 402
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 408, 424
Bse118I RCCGGY 1 cut(s) 347
Bse1I ACTGG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 541
BseDI CCNNGG 1 cut(s) 402
BseJI GATNNNNATC 1 cut(s) 541
BseLI CCNNNNNNNGG 3 cut(s) 118, 408, 424
BseNI ACTGG 1 cut(s) 50
BseXI GCAGC 3 cut(s) 427, 467, 628
BshFI GGCC 1 cut(s) 412
BsiSI CCGG 1 cut(s) 348
BslI CCNNNNNNNGG 3 cut(s) 118, 408, 424
BsmAI GTCTC 1 cut(s) 640
BsmI GAATGC 1 cut(s) 166
BsnI GGCC 1 cut(s) 412
Bsp143I GATC 4 cut(s) 27, 531, 536, 542
Bsp19I CCATGG 1 cut(s) 402
BspACI CCGC 1 cut(s) 155
BspANI GGCC 1 cut(s) 412
BspPI GGATC 3 cut(s) 35, 544, 550
BsrFI RCCGGY 1 cut(s) 347
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 347
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 4 cut(s) 27, 531, 536, 542
BssT1I CCWWGG 1 cut(s) 402
Bst4CI ACNGT 1 cut(s) 36
BstC8I GCNNGC 1 cut(s) 349
BstDSI CCRYGG 1 cut(s) 402
BstEII GGTNACC 1 cut(s) 390
BstHHI GCGC 1 cut(s) 353
BstKTI GATC 4 cut(s) 30, 534, 539, 545
BstMAI GTCTC 1 cut(s) 640
BstMBI GATC 4 cut(s) 27, 531, 536, 542
BstMWI GCNNNNNNNGC 3 cut(s) 161, 365, 613
BstNSI RCATGY 1 cut(s) 440
BstPI GGTNACC 1 cut(s) 390
BstV1I GCAGC 3 cut(s) 427, 467, 628
BstX2I RGATCY 2 cut(s) 536, 542
BstYI RGATCY 2 cut(s) 536, 542
BsuRI GGCC 1 cut(s) 412
BtgI CCRYGG 1 cut(s) 402
Cac8I GCNNGC 1 cut(s) 349
CfoI GCGC 1 cut(s) 353
Cfr10I RCCGGY 1 cut(s) 347
Cfr13I GGNCC 1 cut(s) 410
CviAII CATG 4 cut(s) 24, 383, 403, 437
CviJI RGCY 9 cut(s) 71, 122, 412, 428, 476, 481, 500, 566, 619
CviKI_1 RGCY 9 cut(s) 71, 122, 412, 428, 476, 481, 500, 566, 619
DpnI GATC 4 cut(s) 29, 533, 538, 544
DpnII GATC 4 cut(s) 27, 531, 536, 542
Eco130I CCWWGG 1 cut(s) 402
Eco91I GGTNACC 1 cut(s) 390
EcoO109I RGGNCCY 1 cut(s) 410
EcoO65I GGTNACC 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 402
ErhI CCWWGG 1 cut(s) 402
FaeI CATG 4 cut(s) 27, 386, 406, 440
FatI CATG 4 cut(s) 23, 382, 402, 436
Fnu4HI GCNGC 4 cut(s) 156, 441, 456, 617
Fsp4HI GCNGC 4 cut(s) 156, 441, 456, 617
FspBI CTAG 1 cut(s) 150
GlaI GCGC 1 cut(s) 352
GluI GCNGC 4 cut(s) 156, 441, 456, 617
GsuI CTGGAG 1 cut(s) 567
HaeIII GGCC 1 cut(s) 412
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 353
Hin1II CATG 4 cut(s) 27, 386, 406, 440
Hin6I GCGC 1 cut(s) 351
HinP1I GCGC 1 cut(s) 351
HpaII CCGG 1 cut(s) 348
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 1 cut(s) 141
Hpy188I TCNGA 3 cut(s) 129, 536, 597
Hpy188III TCNNGA 3 cut(s) 260, 540, 584
Hpy8I GTNNAC 1 cut(s) 141
HpyAV CCTTC 3 cut(s) 343, 389, 401
HpyCH4III ACNGT 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 3 cut(s) 161, 365, 613
Hsp92II CATG 4 cut(s) 27, 386, 406, 440
HspAI GCGC 1 cut(s) 351
KroI GCCGGC 1 cut(s) 347
KroNI GCCGGC 1 cut(s) 349
Kzo9I GATC 4 cut(s) 27, 531, 536, 542
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 8 cut(s) 57, 63, 228, 361, 373, 444, 525, 597
Lsp1109I GCAGC 3 cut(s) 427, 467, 628
LweI GCATC 1 cut(s) 388
MaeI CTAG 1 cut(s) 150
MaeIII GTNAC 3 cut(s) 181, 319, 390
MalI GATC 4 cut(s) 29, 533, 538, 544
MboI GATC 4 cut(s) 27, 531, 536, 542
MboII GAAGA 2 cut(s) 98, 476
MflI RGATCY 2 cut(s) 536, 542
MluCI AATT 4 cut(s) 219, 254, 371, 462
MmeI TCCRAC 1 cut(s) 113
MnlI CCTC 1 cut(s) 498
MroNI GCCGGC 1 cut(s) 347
MseI TTAA 3 cut(s) 62, 306, 515
MspI CCGG 1 cut(s) 348
Mva1269I GAATGC 1 cut(s) 166
MwoI GCNNNNNNNGC 3 cut(s) 161, 365, 613
NaeI GCCGGC 1 cut(s) 349
NcoI CCATGG 1 cut(s) 402
NdeII GATC 4 cut(s) 27, 531, 536, 542
NgoMIV GCCGGC 1 cut(s) 347
NlaIII CATG 4 cut(s) 27, 386, 406, 440
NmeAIII GCCGAG 1 cut(s) 457
NspI RCATGY 1 cut(s) 440
PctI GAATGC 1 cut(s) 166
PdiI GCCGGC 1 cut(s) 349
PkrI GCNGC 4 cut(s) 157, 442, 457, 618
PspEI GGTNACC 1 cut(s) 390
PspPI GGNCC 1 cut(s) 410
PsuI RGATCY 2 cut(s) 536, 542
SaqAI TTAA 3 cut(s) 62, 306, 515
SatI GCNGC 4 cut(s) 156, 441, 456, 617
Sau3AI GATC 4 cut(s) 27, 531, 536, 542
Sau96I GGNCC 1 cut(s) 410
SetI ASST 9 cut(s) 73, 151, 229, 247, 478, 502, 508, 568, 621
SfaNI GCATC 1 cut(s) 388
Sse9I AATT 4 cut(s) 219, 254, 371, 462
SsiI CCGC 1 cut(s) 155
SspI AATATT 2 cut(s) 20, 276
SspMI CTAG 1 cut(s) 150
StyI CCWWGG 1 cut(s) 402
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 557
TasI AATT 4 cut(s) 219, 254, 371, 462
TauI GCSGC 1 cut(s) 158
Tru1I TTAA 3 cut(s) 62, 306, 515
Tru9I TTAA 3 cut(s) 62, 306, 515
TseI GCWGC 3 cut(s) 440, 455, 616
TspDTI ATGAA 6 cut(s) 12, 28, 315, 371, 484, 542
XapI RAATTY 3 cut(s) 219, 254, 462
XceI RCATGY 1 cut(s) 440
XspI CTAG 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.