FvH4_c3g00021

A subunit of NADH dehydrogenase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
contig_3
OG0020165
Physical Location & Seq
Forward (+)
15613 .. 17852
2240 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_c3g00021.t1

Sequence Viewer

Length: 2211 bp
ATGGAATATACATATCAATATTCATGGATCATACCGTTCATTCCACTTCCAGTTCCTATGTTAATAGGAGCAGGGCTTCTACTTTTTCCAACGGCAACTAAAAATCTTCGCCGTATGTGGGCTTTTCCGAGTGTTTTATTATTAAGTATAGTTTTGCTTTTTTCAGCCCATTTCTTTATTCAGCAACTAAATAACAATTCTGGCTATCAATATGTATGGTCTTGGACCATCAATAATGATTTTTCTTTAGAGTTCGGCTCCTTGGTTGATCCACTTACTTCTATTATGTCAATATTAATCACTAGTGTTGGGGTTATTGTTCTTATTTATAGTGACAATTATATGTCTCATGATCGGGGATATGTGAGATTTTTTGCTTATATGAGTTTTTTCAATACTTCAATGTTGGGATTAGTTACTAGTTCTAATTTAATACAAATTTATATTTTTTGGGAATTGGTTGGAATGTGTTCTTATCTATTAATAGGTTTTTGGTTCACCCGACCCAGCGCGGCAAATGCTTGTCAAAAAGCGTTTGTAACTAATCGTGTCGGGGATTTTGGGTTATTATTAGGAATTTTAGGTTTTTATTGGATAACAGGTAGTTTTGAATTTCGAGATTTGTTTGAAATATTCAATAATTTGGTTTATAATAATGAAGTTAATACTTTATTTGTTACTTTCTGTGCCTTCCTTTTATTTGCCGGCGCAGTTGCTAAATCTGCTCAATTTCCCCTTCATGTCTGGTTACCCGATGCCATGGAAGGGCCTACCCCTATTTCGGCTCTTATACATGCTGCTACCATGGTAGCAGCAGGGATTTTTCTTGTAGCTAGGCTTCTTCCTCTTTTCATAGTTATACCTTACATATTAAATATAATATCTTTGATAGGTATAATAACATTATTTTTAGGAGCTACTTTAGCTCTTGCTCAAAAAGATATTAAGAGAGGTTTAGCTTATTCTACAATGTCTCAATTGGGTTATATGATGTTAGCTTTAGGTATGGGTTCTTATCGAGCTGCTTTATTTCATTTGATTACTCATGCTTATTCGAAAGCATTGTTGTTTTTAGGATCTGGATCTATTATTCATTCGATGGAAGCTATTGTTGGATATTCTCCAGATAAAAGTCAGAATATGGTTCTTATGGGTGGTTTAAGAAAACATGTACCAATTACAAAAACGTCTTTTTTATTAGGTACACTTTCTCTTTGTGGTATTCCACCTCTGGCTTGTTTTTGGTCCAAAGATGAAATTCTTAATGATAGTTGGTTGTATTCACCAATTTTTGCAATAATAGCTTATTCTACGGCAGGATTAACCGCTTTTTATATGTTTCGGATCTATTTACTTACTTTTGAAGGACATTTAAACGTTTATTTTCAAAATTACAGTGGCAAAAAAAGCAGCTCATTCTATTCAATGTCTCTATGGGGTAAAGAAGGACCTAAAATTATGAAAAAAAACTTTCGTTTATTCGATTTATTAATGATGAAAAACAACGAAGGGGTTACTTTTTTAAACACATATCGAATTGATAGTAATGAAAATGTAAGAAGCATGGTGCAGCCTTTTATTAGTATTACTCATTTTAGCACTAAAAAAACTTTCTCATATCCCTATGAGTCGGACAACACTATGCTATTTCCCATGCTTGTATTGGTTTTATTTACGTTATTCGTTGGGGCCGTAGGAATTCCTTTCTTCAATCAGGAAGGGGTGGGGTTAGATATATTATCCAAATTATTAACTCCGTCCATAAACCTTTTACATCAAAACCGAACCCATTCTGTCGATTGGTATGAATTTCTTATAAACGCAGTTTTTTCAGTCAGTATAGCTTATTGCGGAATACTTATAGCGTCCTTTTTATATAAGCCTGTTTATTCATCTTTACAAAATTTGAATTTATTTAATTCATTTGTTAAAAGGGTTCCTAATAAAATGCAAGTTTTGGGAGGGAAAATAATAAATGTGATATATGATTGGTCATATAATCGCGGTTACATAGATGTTTTTTATACAATCTCCTTAACTAAGTGTATAAGAAAATTAGCCGAATTAACTCATTTTTTTGATAGACGAGTAATCGATGGAATTACGAATGGAGTAGGTATTGTAAGTTTTTTCGTAGGAGAATGTATCAAATATGTAGGGGGCGGTCGAATTTCTTCTTATCTTTTAGTTGATTTTTTTTTATTTTTTTAA

Protein Analysis

737

Amino Acids

83.25

Weight (kDa)

8.94

Isoelectric Point (pI)

27.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Proton_antipo_N PF00662 75 - 125 1.1e-15 NADH-Ubiquinone oxidoreductase (complex I), chain 5 N-terminus
Proton_antipo_M PF00361 141 - 441 7.1e-95 NADH:quinone oxidoreductase/Mrp antiporter, TM
Proton_antipo_C PF01010 448 - 694 3.1e-114 NADH-dehyrogenase subunit F, TMs, (complex I) C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0020165)

Species Orthologous Gene IDs
arabidopsis_thaliana ATCG01010
fragaria_vesca FvH4_c3g00021
malus_domestica MD00G1121700.v1.1
prunus_persica Prupe.2G145900_v2.0.a1
rosa_chinensis RchiOBHm_CPg0501681
rosa_laevigata RLG00000000210

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 651, 1817
AccII CGCG 2 cut(s) 512, 2004
AciI CCGC 5 cut(s) 512, 1326, 1851, 2004, 2163
AclI AACGTT 1 cut(s) 1377
AclWI GGATC 5 cut(s) 35, 263, 1084, 1090, 1352
AcsI RAATTY 9 cut(s) 438, 576, 611, 1257, 1698, 1808, 1903, 1909, 2169
AfaI GTAC 2 cut(s) 1173, 1204
AfiI CCNNNNNNNGG 3 cut(s) 118, 765, 781
AflIII ACRYGT 1 cut(s) 1168
AhlI ACTAGT 2 cut(s) 302, 419
AjuI GAANNNNNNNTTGG 2 cut(s) 1095, 1127
Alw26I GTCTC 3 cut(s) 351, 978, 1434
AlwI GGATC 5 cut(s) 35, 263, 1084, 1090, 1352
AoxI GGCC 2 cut(s) 767, 1689
ApeKI GCWGC 5 cut(s) 797, 812, 1022, 1410, 1570
ApoI RAATTY 9 cut(s) 438, 576, 611, 1257, 1698, 1808, 1903, 1909, 2169
AseI ATTAAT 3 cut(s) 296, 482, 1490
Asp700I GAANNNNTTC 3 cut(s) 469, 1789, 2173
AspLEI GCGC 2 cut(s) 512, 710
AspS9I GGNCC 5 cut(s) 225, 767, 1245, 1448, 1689
AsuHPI GGTGA 2 cut(s) 490, 1275
AsuII TTCGAA 1 cut(s) 1055
AvaII GGWCC 3 cut(s) 225, 1245, 1448
BarI GAAGNNNNNNTAC 2 cut(s) 822, 854
BbvI GCAGC 5 cut(s) 784, 824, 1009, 1422, 1582
BccI CCATC 3 cut(s) 236, 1093, 2090
BceAI ACGGC 4 cut(s) 96, 108, 1329, 1676
BcoDI GTCTC 3 cut(s) 351, 978, 1434
BcuI ACTAGT 2 cut(s) 302, 419
BfaI CTAG 3 cut(s) 303, 420, 834
BisI GCNGC 6 cut(s) 513, 798, 813, 1023, 1411, 1571
BlsI GCNGC 6 cut(s) 514, 799, 814, 1024, 1412, 1572
Bme18I GGWCC 3 cut(s) 225, 1245, 1448
BmgT120I GGNCC 5 cut(s) 225, 767, 1245, 1448, 1689
BmiI GGNNCC 3 cut(s) 259, 1690, 1938
BmsI GCATC 1 cut(s) 745
BplI GAGNNNNNCTC 2 cut(s) 242, 274
BpmI CTGGAG 1 cut(s) 1107
Bpu14I TTCGAA 1 cut(s) 1055
Bsa29I ATCGAT 1 cut(s) 2094
BsaBI GATNNNNATC 1 cut(s) 1081
BsaJI CCNNGG 3 cut(s) 261, 759, 804
Bsc4I CCNNNNNNNGG 3 cut(s) 118, 765, 781
Bse118I RCCGGY 1 cut(s) 704
Bse1I ACTGG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 1081
BseCI ATCGAT 1 cut(s) 2094
BseDI CCNNGG 3 cut(s) 261, 759, 804
BseJI GATNNNNATC 1 cut(s) 1081
BseLI CCNNNNNNNGG 3 cut(s) 118, 765, 781
BseNI ACTGG 1 cut(s) 50
BseXI GCAGC 5 cut(s) 784, 824, 1009, 1422, 1582
BseYI CCCAGC 1 cut(s) 506
BsgI GTGCAG 1 cut(s) 1589
Bsh1236I CGCG 2 cut(s) 512, 2004
Bsh1285I CGRYCG 1 cut(s) 2167
BshFI GGCC 2 cut(s) 769, 1691
BshVI ATCGAT 1 cut(s) 2094
BsiEI CGRYCG 1 cut(s) 2167
BsiSI CCGG 1 cut(s) 705
BslI CCNNNNNNNGG 3 cut(s) 118, 765, 781
BsmAI GTCTC 3 cut(s) 351, 978, 1434
BsnI GGCC 2 cut(s) 769, 1691
Bsp119I TTCGAA 1 cut(s) 1055
Bsp143I GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
Bsp19I CCATGG 2 cut(s) 759, 804
BspACI CCGC 5 cut(s) 512, 1326, 1851, 2004, 2163
BspANI GGCC 2 cut(s) 769, 1691
BspDI ATCGAT 1 cut(s) 2094
BspFNI CGCG 2 cut(s) 512, 2004
BspHI TCATGA 1 cut(s) 349
BspLI GGNNCC 3 cut(s) 259, 1690, 1938
BspPI GGATC 5 cut(s) 35, 263, 1084, 1090, 1352
BspT104I TTCGAA 1 cut(s) 1055
BsrFI RCCGGY 1 cut(s) 704
BsrI ACTGG 1 cut(s) 50
BssAI RCCGGY 1 cut(s) 704
BssECI CCNNGG 3 cut(s) 261, 759, 804
BssMI GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
BssT1I CCWWGG 3 cut(s) 261, 759, 804
Bst4CI ACNGT 2 cut(s) 36, 1397
BstBI TTCGAA 1 cut(s) 1055
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 1 cut(s) 2040
BstDSI CCRYGG 2 cut(s) 759, 804
BstEII GGTNACC 1 cut(s) 747
BstFNI CGCG 2 cut(s) 512, 2004
BstHHI GCGC 2 cut(s) 512, 710
BstKTI GATC 6 cut(s) 30, 271, 355, 1079, 1085, 1347
BstMAI GTCTC 3 cut(s) 351, 978, 1434
BstMBI GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
BstMCI CGRYCG 1 cut(s) 2167
BstMWI GCNNNNNNNGC 5 cut(s) 518, 722, 923, 1301, 1407
BstNSI RCATGY 2 cut(s) 797, 1172
BstPI GGTNACC 1 cut(s) 747
BstUI CGCG 2 cut(s) 512, 2004
BstV1I GCAGC 5 cut(s) 784, 824, 1009, 1422, 1582
BstX2I RGATCY 3 cut(s) 1076, 1082, 1344
BstYI RGATCY 3 cut(s) 1076, 1082, 1344
Bsu15I ATCGAT 1 cut(s) 2094
BsuRI GGCC 2 cut(s) 769, 1691
BsuTUI ATCGAT 1 cut(s) 2094
BtgI CCRYGG 2 cut(s) 759, 804
BtsIMutI CAGTG 1 cut(s) 1402
Cac8I GCNNGC 1 cut(s) 706
CciI TCATGA 1 cut(s) 349
CfoI GCGC 2 cut(s) 512, 710
Cfr10I RCCGGY 1 cut(s) 704
Cfr13I GGNCC 5 cut(s) 225, 767, 1245, 1448, 1689
ClaI ATCGAT 1 cut(s) 2094
CseI GACGC 1 cut(s) 1854
Csp6I GTAC 2 cut(s) 1172, 1203
CviQI GTAC 2 cut(s) 1172, 1203
DdeI CTNAG 1 cut(s) 2040
DpnI GATC 6 cut(s) 29, 270, 354, 1078, 1084, 1346
DpnII GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
DraI TTTAAA 2 cut(s) 1374, 1524
Eco130I CCWWGG 3 cut(s) 261, 759, 804
Eco47I GGWCC 3 cut(s) 225, 1245, 1448
Eco91I GGTNACC 1 cut(s) 747
EcoO109I RGGNCCY 2 cut(s) 767, 1448
EcoO65I GGTNACC 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 1698
EcoT14I CCWWGG 3 cut(s) 261, 759, 804
ErhI CCWWGG 3 cut(s) 261, 759, 804
Fnu4HI GCNGC 6 cut(s) 513, 798, 813, 1023, 1411, 1571
Fsp4HI GCNGC 6 cut(s) 513, 798, 813, 1023, 1411, 1571
FspBI CTAG 3 cut(s) 303, 420, 834
GlaI GCGC 2 cut(s) 511, 709
GluI GCNGC 6 cut(s) 513, 798, 813, 1023, 1411, 1571
GsaI CCCAGC 1 cut(s) 510
GsuI CTGGAG 1 cut(s) 1107
HaeIII GGCC 2 cut(s) 769, 1691
HapII CCGG 1 cut(s) 705
HgaI GACGC 1 cut(s) 1854
HhaI GCGC 2 cut(s) 512, 710
Hin6I GCGC 2 cut(s) 510, 708
HinP1I GCGC 2 cut(s) 510, 708
HinfI GANTC 1 cut(s) 1628
HpaII CCGG 1 cut(s) 705
HphI GGTGA 2 cut(s) 490, 1275
Hpy166II GTNNAC 2 cut(s) 498, 1205
Hpy188I TCNGA 4 cut(s) 129, 1137, 1344, 1633
Hpy188III TCNNGA 5 cut(s) 350, 617, 1080, 1124, 1715
Hpy8I GTNNAC 2 cut(s) 498, 1205
HpyAV CCTTC 7 cut(s) 700, 746, 758, 1358, 1439, 1502, 1712
HpyCH4III ACNGT 2 cut(s) 36, 1397
HpyCH4IV ACGT 3 cut(s) 1187, 1377, 1676
HpyCH4V TGCA 3 cut(s) 1295, 1570, 1951
HpyF10VI GCNNNNNNNGC 5 cut(s) 518, 722, 923, 1301, 1407
HpyF3I CTNAG 1 cut(s) 2040
HpySE526I ACGT 3 cut(s) 1187, 1377, 1676
HspAI GCGC 2 cut(s) 510, 708
KroI GCCGGC 1 cut(s) 704
KroNI GCCGGC 1 cut(s) 706
Kzo9I GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
LmnI GCTCC 3 cut(s) 68, 263, 914
Lsp1109I GCAGC 5 cut(s) 784, 824, 1009, 1422, 1582
LweI GCATC 1 cut(s) 745
MaeI CTAG 3 cut(s) 303, 420, 834
MaeII ACGT 3 cut(s) 1187, 1377, 1676
MaeIII GTNAC 7 cut(s) 332, 415, 538, 676, 747, 1513, 2006
MalI GATC 6 cut(s) 29, 270, 354, 1078, 1084, 1346
MboI GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
MboII GAAGA 4 cut(s) 98, 833, 1699, 2166
MfeI CAATTG 1 cut(s) 977
MflI RGATCY 3 cut(s) 1076, 1082, 1344
MlyI GAGTC 1 cut(s) 1637
MmeI TCCRAC 4 cut(s) 113, 442, 1093, 1611
MnlI CCTC 4 cut(s) 855, 944, 1239, 1955
MroNI GCCGGC 1 cut(s) 704
MroXI GAANNNNTTC 3 cut(s) 469, 1789, 2173
MspI CCGG 1 cut(s) 705
MunI CAATTG 1 cut(s) 977
MvnI CGCG 2 cut(s) 512, 2004
MwoI GCNNNNNNNGC 5 cut(s) 518, 722, 923, 1301, 1407
NaeI GCCGGC 1 cut(s) 706
NcoI CCATGG 2 cut(s) 759, 804
NdeII GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
NgoMIV GCCGGC 1 cut(s) 704
NlaIV GGNNCC 3 cut(s) 259, 1690, 1938
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 2 cut(s) 797, 1172
NspV TTCGAA 1 cut(s) 1055
PagI TCATGA 1 cut(s) 349
PciI ACATGT 1 cut(s) 1168
PcsI WCGNNNNNNNCGW 1 cut(s) 1689
PdiI GCCGGC 1 cut(s) 706
PdmI GAANNNNTTC 3 cut(s) 469, 1789, 2173
PkrI GCNGC 6 cut(s) 514, 799, 814, 1024, 1412, 1572
PleI GAGTC 1 cut(s) 1636
PpsI GAGTC 1 cut(s) 1636
PpuMI RGGWCCY 1 cut(s) 1448
PscI ACATGT 1 cut(s) 1168
PshBI ATTAAT 3 cut(s) 296, 482, 1490
PsiI TTATAA 2 cut(s) 651, 1817
Psp1406I AACGTT 1 cut(s) 1377
Psp5II RGGWCCY 1 cut(s) 1448
PspEI GGTNACC 1 cut(s) 747
PspFI CCCAGC 1 cut(s) 506
PspN4I GGNNCC 3 cut(s) 259, 1690, 1938
PspPI GGNCC 5 cut(s) 225, 767, 1245, 1448, 1689
PspPPI RGGWCCY 1 cut(s) 1448
PsuI RGATCY 3 cut(s) 1076, 1082, 1344
RsaI GTAC 2 cut(s) 1173, 1204
RsaNI GTAC 2 cut(s) 1172, 1203
SatI GCNGC 6 cut(s) 513, 798, 813, 1023, 1411, 1571
Sau3AI GATC 6 cut(s) 27, 268, 352, 1076, 1082, 1344
Sau96I GGNCC 5 cut(s) 225, 767, 1245, 1448, 1689
SchI GAGTC 1 cut(s) 1637
SfaNI GCATC 1 cut(s) 745
SfuI TTCGAA 1 cut(s) 1055
SinI GGWCC 3 cut(s) 225, 1245, 1448
SpeI ACTAGT 2 cut(s) 302, 419
SsiI CCGC 5 cut(s) 512, 1326, 1851, 2004, 2163
SspI AATATT 3 cut(s) 20, 294, 633
SspMI CTAG 3 cut(s) 303, 420, 834
StyI CCWWGG 3 cut(s) 261, 759, 804
TaaI ACNGT 2 cut(s) 36, 1397
TaiI ACGT 3 cut(s) 1190, 1380, 1679
TaqI TCGA 9 cut(s) 616, 1018, 1055, 1097, 1482, 1534, 1797, 2094, 2167
TauI GCSGC 1 cut(s) 515
TscAI CASTG 1 cut(s) 1402
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 5 cut(s) 797, 812, 1022, 1410, 1570
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 1746
TspRI CASTG 1 cut(s) 1402
VpaK11BI GGWCC 3 cut(s) 225, 1245, 1448
VspI ATTAAT 3 cut(s) 296, 482, 1490
XapI RAATTY 9 cut(s) 438, 576, 611, 1257, 1698, 1808, 1903, 1909, 2169
XceI RCATGY 2 cut(s) 797, 1172
XcmI CCANNNNNNNNNTGG 1 cut(s) 1660
XmnI GAANNNNTTC 3 cut(s) 469, 1789, 2173
XspI CTAG 3 cut(s) 303, 420, 834
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.