FvH4_1g00650

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014340
Physical Location & Seq
Forward (+)
337394 .. 342823
5430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00650.t3

Sequence Viewer

Length: 2100 bp
ATGTCACTCCTTCCCTCCAAGTTTGGCGCTCTCCACCTCCATCTCCTCCGCCCTCCAAACCCTAACCCTAGCCCTCATCCCCGCCGCCAACCTCTCAAAATCTTCTGCGGCAAGAGGTCCGGCAGGCAGAGGTACCCGTCGGAGAGGAAGGTGCTCAAATTGAAGCACAAGGATATCATCGGCAACGTCAAGAACAAATACCAAGGCGTCTGGCGGCTCTCCAAGCTCGGCGTTCCCGTCGACCAAGACCCCGGCAAGGACTTCCTTGGCGTCTCCCAAAGCTTGCTTGAGGAAATTGCCAAAGTGCTCGAGTTTCCGGTCGCTTCAATGCTGCCGTCGGAGGCGTTCACGGTGGTTCGGAAATCTTTTGATGCAAGGAAGAGGCTGAAAGAACCGAAATTCGTGTACATTGTGGAGATGGATGTTGATAAGCTATTGAGTTTGGAGCCTCGTGCTTGGGACTTCATTTCGGAGTTGGAGCCTAAGGTTGGGCTGGTAGAGCACATGCCTCAAGCCAGGAAATCTAGAGATTTGACGAGTATTATACATGACTTGAAAGAAGTAGATCAGAGTTCAGTTTCGGGAGGAAGTGGGAATACAATGTTGAATGGGTCTCAAGGATTGTACAGATTTTCAACAGCTAGGAAACCCAAAATTGCAGTAGTGGGTAGTGGACCAGCTGGGCTGTTTGCTGCCCTTGTTCTTGCGGAATTTGGTGCAGATGTCACCTTAATAGAAAGAGGTCAGCCTGTTGAACAGCGGGGGCGTGACATTGGTGCTTTGGCTGTCCGCCGGATCTTGCAAACAGAAAGCAATTTCTGCTTCGGAGAGGGTGGTGCAGGTACCTGGAGTGATGGAAAGCTGGTGACTAGGATTGGAAGAAACAGTGGGAGTGTTTTGGCGGTTATGGAAACTCTAGTTCAATTTGGAGCTCCAGAAGGAATTTTGGTTAATGGAAAGCCTCATTTGGGAACAGATAAGTTGGTTCCACTACTTCGCAATTTCCGGCAACATCTACTAAAGCTTGGTGTCACCATCAAGTTTGGGACAAGGGTCGATGATCTTCTTGTAGATAATGAACAAGTTGTAGGTGTCAAAGTTTCTGATTCAGTTGACAGGTTACAGTCTAGCAGCAAGAAATGGGAATACGATGCTGTTGTTCTGGCTGTTGGACATTCTGCGCGTGATCTTTATCAAACACTTCTGTCTCATAACATTGAGTTGATCCCAAAAGACTTTGCTGTTGGCTTGCGGATTGAGCATCCTCAAGAAGTTGTAAACAGCTTACAGTATTCTGGATTGGCAACTGAGGTTTGCAGAGGACGCGGTAAAGTACCAGTGGCAGATTACAAGGTTGCAAAGTATGTAAGTGGAGAGAAGGGGGCTGAACCTTTGCATGCAACAAGTCGAAGCTGCTATTCCTTCTGTATGTGTCCTGGTGGTCAGGTTGTTCTGACAAGCACAAATCCATCTGAAATCTGTGTCAATGGCATGTCATTTTCTCGACGTGACTCAAAGTGGGCAAATGCTGCACTCGTTGTGACTGTCTCAACGAAAGATTTTGGTGCATTGAATCTCCATGGACCTCTAGCAGGGGTTGAATTTCAGAGAGAATTTGAGCAAAGAGCAGCAAGAATGGGAGGTGGAAATTTCGTGGTGCCTGTGCAGACAGTTACAGATTTTATGGACAATAAATTATCGGCAACGTCTGTGCCACAGTCAAGTTATCGATTAGGAGTGAAGGCAGCAAACCTTCACAAGATCTTTCCTGATTATGTAACAGAGACTTTGCAACAGTCAATCTCAGCATTTGACAAAGAGTTACCAGGCTTTATCTCCAAAGAGGCCCTTCTTCACGGAGTGGAGACTAGGACCAGTTCTCCTATCCAGATTCCCCGCAGTGTTGAAACTTACGAGAGCACAACTTTGAAAGGTCTCTACCCTGTTGGTGAAGGAGCAGGTTATGCTGGAGGGATTGTAAGTGCAGCCGTGGATGGTATGCATGCTGGTTTTGCAGTGGCAAAGAATTTTGGATTGTGTAGTGATGGCATAGAGTCAATTTTGGGTAAGGCTCGAAGTGCTGCATTTTTGGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_2 FAD_binding_3 Pyr_redox Pyr_redox_2

Protein Analysis

700

Amino Acids

76.14

Weight (kDa)

9.19

Isoelectric Point (pI)

38.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 218 - 397 5.7e-09 Pyridine nucleotide-disulphide oxidoreductase
FAD_binding_3 PF01494 218 - 255 5.3e-06 FAD binding domain
FAD-depend_C PF21688 412 - 625 6.9e-68 FAD-dependent protein, C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30720
fragaria_vesca FvH4_1g00650 FvH4_1g00650
malus_domestica MD02G1005300.v1.1
prunus_persica Prupe.7G265700_v2.0.a1 Prupe.7G265700_v2.0.a1
pyrus_communis pycom02g00410
rosa_chinensis RchiOBHm_Chr2g0085271
rosa_laevigata RLG00000015673
rosa_multiflora Rmu_sc0008510.1_g000007
rosa_roxburghii Rroxscaffold_2G00155500
rosa_rugosa Rorug01G0458800 Rorug01G0458900
rosa_samantha Rh2AG007500 Rh2BG008100 Rh2CG008900
rosa_wichuraiana Rw2G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 830, 1949
Acc65I GGTACC 2 cut(s) 132, 842
AccB1I GGYRCC 3 cut(s) 132, 842, 1657
AccI GTMKAC 1 cut(s) 240
AccII CGCG 2 cut(s) 1183, 1326
AclWI GGATC 2 cut(s) 803, 1219
AcsI RAATTY 7 cut(s) 398, 710, 942, 1601, 1613, 1648, 2026
AcyI GRCGYC 2 cut(s) 207, 270
AdeI CACNNNGTG 1 cut(s) 1861
AfaI GTAC 6 cut(s) 134, 407, 626, 844, 1335, 2096
AfiI CCNNNNNNNGG 4 cut(s) 256, 488, 968, 1592
AjiI CACGTC 1 cut(s) 1508
AjnI CCWGG 4 cut(s) 515, 845, 1435, 1825
Alw21I GWGCWC 5 cut(s) 156, 309, 504, 934, 1922
Alw26I GTCTC 7 cut(s) 277, 618, 1212, 1552, 1778, 1859, 1940
AlwI GGATC 2 cut(s) 803, 1219
Ama87I CYCGRG 1 cut(s) 308
AoxI GGCC 1 cut(s) 1845
ApeKI GCWGC 9 cut(s) 331, 692, 1131, 1413, 1529, 1628, 1745, 1985, 2081
ApoI RAATTY 7 cut(s) 398, 710, 942, 1601, 1613, 1648, 2026
Asp718I GGTACC 2 cut(s) 132, 842
AspLEI GCGC 2 cut(s) 29, 1183
AspS9I GGNCC 5 cut(s) 117, 674, 1583, 1846, 1872
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 4 cut(s) 718, 877, 1024, 1961
AvaI CYCGRG 1 cut(s) 308
AvaII GGWCC 4 cut(s) 117, 674, 1583, 1872
AxyI CCTNAGG 1 cut(s) 483
BanI GGYRCC 3 cut(s) 132, 842, 1657
BanII GRGCYC 1 cut(s) 934
BarI GAAGNNNNNNTAC 2 cut(s) 580, 612
BauI CACGAG 1 cut(s) 450
Bbv12I GWGCWC 5 cut(s) 156, 309, 504, 934, 1922
BbvI GCAGC 9 cut(s) 318, 679, 1143, 1400, 1516, 1640, 1757, 1997, 2068
BccI CCATC 7 cut(s) 48, 412, 848, 1043, 1477, 1988, 2039
BceAI ACGGC 2 cut(s) 319, 1973
BciT130I CCWGG 4 cut(s) 517, 847, 1437, 1827
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 7 cut(s) 277, 618, 1212, 1552, 1778, 1859, 1940
BfaI CTAG 9 cut(s) 69, 525, 642, 870, 917, 1128, 1589, 1869, 2098
BfoI RGCGCY 1 cut(s) 30
BfuAI ACCTGC 2 cut(s) 830, 1949
BglII AGATCT 1 cut(s) 1761
BmcAI AGTACT 1 cut(s) 2096
Bme1390I CCNGG 5 cut(s) 252, 517, 847, 1437, 1827
Bme18I GGWCC 4 cut(s) 117, 674, 1583, 1872
BmeT110I CYCGRG 1 cut(s) 308
BmgBI CACGTC 1 cut(s) 1508
BmgT120I GGNCC 5 cut(s) 117, 674, 1583, 1846, 1872
BmiI GGNNCC 6 cut(s) 134, 447, 480, 844, 987, 1659
BmrFI CCNGG 5 cut(s) 252, 517, 847, 1437, 1827
BmsI GCATC 3 cut(s) 361, 1141, 1270
BoxI GACNNNNGTC 1 cut(s) 1052
BpmI CTGGAG 3 cut(s) 868, 918, 1989
BpuEI CTTGAG 4 cut(s) 308, 495, 600, 1251
BpuMI CCSGG 1 cut(s) 252
Bsa29I ATCGAT 1 cut(s) 1729
BsaBI GATNNNNATC 1 cut(s) 1191
BsaHI GRCGYC 2 cut(s) 207, 270
BsaI GGTCTC 2 cut(s) 618, 1940
BsaJI CCNNGG 5 cut(s) 202, 250, 265, 1579, 1989
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 4 cut(s) 1864, 1894, 1962, 1992
Bsc4I CCNNNNNNNGG 4 cut(s) 256, 488, 968, 1592
Bse1I ACTGG 2 cut(s) 1337, 1875
Bse21I CCTNAGG 1 cut(s) 483
Bse8I GATNNNNATC 1 cut(s) 1191
BseBI CCWGG 4 cut(s) 517, 847, 1437, 1827
BseCI ATCGAT 1 cut(s) 1729
BseDI CCNNGG 5 cut(s) 202, 250, 265, 1579, 1989
BseGI GGATG 4 cut(s) 76, 427, 1261, 1999
BseJI GATNNNNATC 1 cut(s) 1191
BseLI CCNNNNNNNGG 4 cut(s) 256, 488, 968, 1592
BseMII CTCAG 2 cut(s) 1299, 1818
BseNI ACTGG 2 cut(s) 1337, 1875
BseRI GAGGAG 1 cut(s) 35
BseXI GCAGC 9 cut(s) 318, 679, 1143, 1400, 1516, 1640, 1757, 1997, 2068
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 5 cut(s) 738, 858, 1515, 1685, 2004
Bsh1236I CGCG 2 cut(s) 1183, 1326
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 1 cut(s) 1847
BshNI GGYRCC 3 cut(s) 132, 842, 1657
BshVI ATCGAT 1 cut(s) 1729
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 5 cut(s) 156, 309, 504, 934, 1922
BsiHKCI CYCGRG 1 cut(s) 308
BsiSI CCGG 5 cut(s) 120, 252, 317, 793, 1006
BslFI GGGAC 2 cut(s) 473, 1060
BslI CCNNNNNNNGG 4 cut(s) 256, 488, 968, 1592
BsmAI GTCTC 7 cut(s) 277, 618, 1212, 1552, 1778, 1859, 1940
BsmBI CGTCTC 1 cut(s) 277
BsmFI GGGAC 2 cut(s) 473, 1060
BsnI GGCC 1 cut(s) 1847
Bso31I GGTCTC 2 cut(s) 618, 1940
BsoBI CYCGRG 1 cut(s) 308
Bsp1286I GDGCHC 5 cut(s) 156, 309, 504, 934, 1922
Bsp1407I TGTACA 2 cut(s) 405, 624
Bsp143I GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
Bsp19I CCATGG 1 cut(s) 1579
BspANI GGCC 1 cut(s) 1847
BspCNI CTCAG 2 cut(s) 1300, 1817
BspDI ATCGAT 1 cut(s) 1729
BspFNI CGCG 2 cut(s) 1183, 1326
BspLI GGNNCC 6 cut(s) 134, 447, 480, 844, 987, 1659
BspMI ACCTGC 2 cut(s) 830, 1949
BspPI GGATC 2 cut(s) 803, 1219
BspT107I GGYRCC 3 cut(s) 132, 842, 1657
BspTNI GGTCTC 2 cut(s) 618, 1940
BsrGI TGTACA 2 cut(s) 405, 624
BsrI ACTGG 2 cut(s) 1337, 1875
BssECI CCNNGG 5 cut(s) 202, 250, 265, 1579, 1989
BssMI GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
BssNI GRCGYC 2 cut(s) 207, 270
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 3 cut(s) 202, 265, 1579
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 4 cut(s) 517, 847, 1437, 1827
Bst4CI ACNGT 8 cut(s) 352, 887, 1125, 1290, 1546, 1672, 1719, 1797
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 2 cut(s) 207, 270
BstAPI GCANNNNNTGC 3 cut(s) 819, 1529, 1964
BstAUI TGTACA 2 cut(s) 405, 624
BstC8I GCNNGC 5 cut(s) 125, 284, 1250, 1398, 2004
BstDEI CTNAG 3 cut(s) 483, 1308, 1804
BstDSI CCRYGG 2 cut(s) 1579, 1989
BstENI CCTNNNNNAGG 1 cut(s) 1590
BstF5I GGATG 4 cut(s) 76, 427, 1261, 1999
BstFNI CGCG 2 cut(s) 1183, 1326
BstH2I RGCGCY 1 cut(s) 30
BstHHI GCGC 2 cut(s) 29, 1183
BstKTI GATC 6 cut(s) 568, 798, 1063, 1189, 1227, 1764
BstMAI GTCTC 7 cut(s) 277, 618, 1212, 1552, 1778, 1859, 1940
BstMBI GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 9 cut(s) 223, 499, 819, 1258, 1323, 1529, 1964, 2012, 2078
BstNI CCWGG 4 cut(s) 517, 847, 1437, 1827
BstNSI RCATGY 4 cut(s) 508, 1400, 1495, 2006
BstPAI GACNNNNGTC 1 cut(s) 1052
BstSCI CCNGG 5 cut(s) 250, 515, 845, 1435, 1825
BstUI CGCG 2 cut(s) 1183, 1326
BstV1I GCAGC 9 cut(s) 318, 679, 1143, 1400, 1516, 1640, 1757, 1997, 2068
BstX2I RGATCY 2 cut(s) 795, 1761
BstYI RGATCY 2 cut(s) 795, 1761
Bsu15I ATCGAT 1 cut(s) 1729
Bsu36I CCTNAGG 1 cut(s) 483
BsuRI GGCC 1 cut(s) 1847
BsuTUI ATCGAT 1 cut(s) 1729
BtgI CCRYGG 2 cut(s) 1579, 1989
BtrI CACGTC 1 cut(s) 1508
BtsCI GGATG 4 cut(s) 76, 427, 1261, 1999
BtsI GCAGTG 2 cut(s) 1906, 2022
BtsIMutI CAGTG 4 cut(s) 892, 1344, 1906, 2022
BveI ACCTGC 2 cut(s) 830, 1949
Cac8I GCNNGC 5 cut(s) 125, 284, 1250, 1398, 2004
CfoI GCGC 2 cut(s) 29, 1183
Cfr13I GGNCC 5 cut(s) 117, 674, 1583, 1846, 1872
ClaI ATCGAT 1 cut(s) 1729
CseI GACGC 3 cut(s) 196, 259, 1332
Csp6I GTAC 6 cut(s) 133, 406, 625, 843, 1334, 2095
CviAII CATG 6 cut(s) 505, 548, 1397, 1492, 1580, 2003
CviQI GTAC 6 cut(s) 133, 406, 625, 843, 1334, 2095
DdeI CTNAG 3 cut(s) 483, 1308, 1804
DpnI GATC 6 cut(s) 567, 797, 1062, 1188, 1226, 1763
DpnII GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
DraIII CACNNNGTG 1 cut(s) 1861
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
EciI GGCGGA 2 cut(s) 38, 779
Ecl136II GAGCTC 1 cut(s) 932
Eco130I CCWWGG 3 cut(s) 202, 265, 1579
Eco24I GRGCYC 1 cut(s) 934
Eco31I GGTCTC 2 cut(s) 618, 1940
Eco32I GATATC 1 cut(s) 175
Eco47I GGWCC 4 cut(s) 117, 674, 1583, 1872
Eco53kI GAGCTC 1 cut(s) 932
Eco81I CCTNAGG 1 cut(s) 483
Eco88I CYCGRG 1 cut(s) 308
EcoICRI GAGCTC 1 cut(s) 932
EcoNI CCTNNNNNAGG 1 cut(s) 1590
EcoO109I RGGNCCY 1 cut(s) 1846
EcoRII CCWGG 4 cut(s) 515, 845, 1435, 1825
EcoRV GATATC 1 cut(s) 175
EcoT14I CCWWGG 3 cut(s) 202, 265, 1579
EcoT22I ATGCAT 1 cut(s) 2004
EcoT38I GRGCYC 1 cut(s) 934
ErhI CCWWGG 3 cut(s) 202, 265, 1579
Esp3I CGTCTC 1 cut(s) 277
FaeI CATG 6 cut(s) 508, 551, 1400, 1495, 1583, 2006
FalI AAGNNNNNCTT 2 cut(s) 1833, 1865
FaqI GGGAC 2 cut(s) 473, 1060
FatI CATG 6 cut(s) 504, 547, 1396, 1491, 1579, 2002
FauI CCCGC 3 cut(s) 89, 753, 1904
FblI GTMKAC 1 cut(s) 240
FokI GGATG 4 cut(s) 63, 434, 1248, 2006
FriOI GRGCYC 1 cut(s) 934
FspBI CTAG 9 cut(s) 69, 525, 642, 870, 917, 1128, 1589, 1869, 2098
GlaI GCGC 2 cut(s) 28, 1182
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 3 cut(s) 868, 918, 1989
HaeII RGCGCY 1 cut(s) 30
HaeIII GGCC 1 cut(s) 1847
HapII CCGG 5 cut(s) 120, 252, 317, 793, 1006
HgaI GACGC 3 cut(s) 196, 259, 1332
HhaI GCGC 2 cut(s) 29, 1183
Hin1I GRCGYC 2 cut(s) 207, 270
Hin1II CATG 6 cut(s) 508, 551, 1400, 1495, 1583, 2006
Hin6I GCGC 2 cut(s) 27, 1181
HinP1I GCGC 2 cut(s) 27, 1181
HincII GTYRAC 2 cut(s) 241, 1114
HindII GTYRAC 2 cut(s) 241, 1114
HindIII AAGCTT 2 cut(s) 280, 1022
HinfI GANTC 5 cut(s) 1106, 1511, 1573, 1891, 2054
HpaII CCGG 5 cut(s) 120, 252, 317, 793, 1006
HphI GGTGA 4 cut(s) 718, 877, 1024, 1961
Hpy166II GTNNAC 6 cut(s) 241, 348, 406, 674, 1114, 1279
Hpy188III TCNNGA 9 cut(s) 190, 525, 582, 935, 1268, 1296, 1503, 1769, 1888
Hpy8I GTNNAC 6 cut(s) 241, 348, 406, 674, 1114, 1279
Hpy99I CGWCG 4 cut(s) 142, 242, 340, 1509
HpyAV CCTTC 9 cut(s) 20, 142, 932, 1372, 1432, 1735, 1763, 1859, 1946
HpyCH4III ACNGT 8 cut(s) 352, 887, 1125, 1290, 1546, 1672, 1719, 1797
HpyCH4IV ACGT 3 cut(s) 186, 1507, 1706
HpyF10VI GCNNNNNNNGC 9 cut(s) 223, 499, 819, 1258, 1323, 1529, 1964, 2012, 2078
HpyF3I CTNAG 3 cut(s) 483, 1308, 1804
HpySE526I ACGT 3 cut(s) 186, 1507, 1706
Hsp92I GRCGYC 2 cut(s) 207, 270
Hsp92II CATG 6 cut(s) 508, 551, 1400, 1495, 1583, 2006
HspAI GCGC 2 cut(s) 27, 1181
KpnI GGTACC 2 cut(s) 136, 846
Kzo9I GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
LmnI GCTCC 5 cut(s) 445, 478, 929, 937, 1955
Lsp1109I GCAGC 9 cut(s) 318, 679, 1143, 1400, 1516, 1640, 1757, 1997, 2068
LweI GCATC 3 cut(s) 361, 1141, 1270
MaeI CTAG 9 cut(s) 69, 525, 642, 870, 917, 1128, 1589, 1869, 2098
MaeII ACGT 3 cut(s) 186, 1507, 1706
MalI GATC 6 cut(s) 567, 797, 1062, 1188, 1226, 1763
MboI GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
MboII GAAGA 5 cut(s) 94, 391, 891, 1055, 1844
MflI RGATCY 2 cut(s) 795, 1761
MhlI GDGCHC 5 cut(s) 156, 309, 504, 934, 1922
MlyI GAGTC 2 cut(s) 1505, 2063
MmeI TCCRAC 4 cut(s) 120, 318, 456, 1150
Mph1103I ATGCAT 1 cut(s) 2004
MseI TTAA 2 cut(s) 731, 951
MspA1I CMGCKG 2 cut(s) 680, 760
MspI CCGG 5 cut(s) 120, 252, 317, 793, 1006
MspR9I CCNGG 5 cut(s) 252, 517, 847, 1437, 1827
MvaI CCWGG 4 cut(s) 517, 847, 1437, 1827
MvnI CGCG 2 cut(s) 1183, 1326
MwoI GCNNNNNNNGC 9 cut(s) 223, 499, 819, 1258, 1323, 1529, 1964, 2012, 2078
NciI CCSGG 1 cut(s) 252
NcoI CCATGG 1 cut(s) 1579
NdeII GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
NlaIII CATG 6 cut(s) 508, 551, 1400, 1495, 1583, 2006
NlaIV GGNNCC 6 cut(s) 134, 447, 480, 844, 987, 1659
NmeAIII GCCGAG 1 cut(s) 207
NmuCI GTSAC 7 cut(s) 3, 724, 767, 865, 1030, 1508, 1540
NsiI ATGCAT 1 cut(s) 2004
NspI RCATGY 4 cut(s) 508, 1400, 1495, 2006
PaeI GCATGC 2 cut(s) 1400, 2006
PaeR7I CTCGAG 1 cut(s) 308
PcsI WCGNNNNNNNCGW 1 cut(s) 234
PfeI GAWTC 3 cut(s) 1106, 1573, 1891
PleI GAGTC 2 cut(s) 1505, 2062
PpsI GAGTC 2 cut(s) 1505, 2062
PshAI GACNNNNGTC 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 934
Psp6I CCWGG 4 cut(s) 515, 845, 1435, 1825
PspFI CCCAGC 1 cut(s) 680
PspGI CCWGG 4 cut(s) 515, 845, 1435, 1825
PspN4I GGNNCC 6 cut(s) 134, 447, 480, 844, 987, 1659
PspPI GGNCC 5 cut(s) 117, 674, 1583, 1846, 1872
PspXI VCTCGAGB 1 cut(s) 308
PsuI RGATCY 2 cut(s) 795, 1761
PvuII CAGCTG 1 cut(s) 680
RsaI GTAC 6 cut(s) 134, 407, 626, 844, 1335, 2096
RsaNI GTAC 6 cut(s) 133, 406, 625, 843, 1334, 2095
SacI GAGCTC 1 cut(s) 934
SalI GTCGAC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 731, 951
Sau3AI GATC 6 cut(s) 565, 795, 1060, 1186, 1224, 1761
Sau96I GGNCC 5 cut(s) 117, 674, 1583, 1846, 1872
ScaI AGTACT 1 cut(s) 2096
SchI GAGTC 2 cut(s) 1505, 2063
ScrFI CCNGG 5 cut(s) 252, 517, 847, 1437, 1827
SduI GDGCHC 5 cut(s) 156, 309, 504, 934, 1922
SfaNI GCATC 3 cut(s) 361, 1141, 1270
Sfr274I CTCGAG 1 cut(s) 308
SinI GGWCC 4 cut(s) 117, 674, 1583, 1872
SlaI CTCGAG 1 cut(s) 308
SmlI CTYRAG 5 cut(s) 287, 308, 510, 615, 1266
SmoI CTYRAG 5 cut(s) 287, 308, 510, 615, 1266
SphI GCATGC 2 cut(s) 1400, 2006
SspMI CTAG 9 cut(s) 69, 525, 642, 870, 917, 1128, 1589, 1869, 2098
SstI GAGCTC 1 cut(s) 934
StyD4I CCNGG 5 cut(s) 250, 515, 845, 1435, 1825
StyI CCWWGG 3 cut(s) 202, 265, 1579
TaaI ACNGT 8 cut(s) 352, 887, 1125, 1290, 1546, 1672, 1719, 1797
TaiI ACGT 3 cut(s) 189, 1510, 1709
TaqI TCGA 7 cut(s) 240, 309, 1056, 1408, 1504, 1729, 2074
TatI WGTACW 3 cut(s) 405, 624, 2094
TauI GCSGC 3 cut(s) 87, 111, 217
TfiI GAWTC 3 cut(s) 1106, 1573, 1891
Tru1I TTAA 2 cut(s) 731, 951
Tru9I TTAA 2 cut(s) 731, 951
TscAI CASTG 4 cut(s) 892, 1344, 1906, 2022
TseFI GTSAC 7 cut(s) 3, 724, 767, 865, 1030, 1508, 1540
TseI GCWGC 9 cut(s) 331, 692, 1131, 1413, 1529, 1628, 1745, 1985, 2081
Tsp45I GTSAC 7 cut(s) 3, 724, 767, 865, 1030, 1508, 1540
TspDTI ATGAA 2 cut(s) 454, 1092
TspGWI ACGGA 1 cut(s) 1872
TspRI CASTG 4 cut(s) 892, 1344, 1906, 2022
VpaK11BI GGWCC 4 cut(s) 117, 674, 1583, 1872
XagI CCTNNNNNAGG 1 cut(s) 1590
XapI RAATTY 7 cut(s) 398, 710, 942, 1601, 1613, 1648, 2026
XbaI TCTAGA 1 cut(s) 524
XceI RCATGY 4 cut(s) 508, 1400, 1495, 2006
XhoI CTCGAG 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 9 cut(s) 69, 525, 642, 870, 917, 1128, 1589, 1869, 2098
ZrmI AGTACT 1 cut(s) 2096
Zsp2I ATGCAT 1 cut(s) 2004
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.