Rroxscaffold_2G00155500

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014340
Physical Location & Seq
Reverse (-)
91908547 .. 91913207
4661 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00155500.1

Sequence Viewer

Length: 2133 bp
ATGTCACTCCTTCCCTCCAAGTTTGGCTCTCTCCACCTCCATCTCCTCCGCCCTCCAAACCCAAACCCTAACCCTAGCCCTCATCCTCGACGCCACACACTCAAGATCTCCAGCGGCAAGAGGTCCGGCAGGCAGAGATACCCGTCGGAGAGGAAGGTGCTCAAGTTGAAGCACAAGGATATCGTCGGGAACGTCAAGAACAAATACGAGGGCATTTGGAGGCTCTCCAAGCTCGCAGTTCCGGTCGACCGTGACCCCGGAAAGGACTTCCTCGGCGTCTCCCAGAGCTTGCTTGAAGAAATTGCCAAAGTGCTCGAGTTTCCGGTTGCTTCAATGCTGCCGTCGGAGGCCTTCACCGTGGTTCGGAAATCTTTTGATGCAAGGAAGAGGTTGAAAGAACCGAAATTCGTTTACGTAGTGGAAATGGATGTTGACAAGCTATTGAGTTTAGAGCCTCGTGCTTGGGACTTCATTTCGGAGTTGGAGCCTAAGGTTGGGCTGGTAGAACACATGCCTCAAGCCAGGATATCTGGAGATTTGACCAGTATTATACATGGCTTGAAAGAAGTGCATCAAAGTTCAGTTTCTGGAGAAAGTGGAAATACAATGCTGAATGGGTCTCAAGGATCGTACAAATTTCCACCGGCTAGAAAACCCAAGATTGCGGTAGTGGGCAGTGGACCATCTGGGCTGTTTGCTGCCCTTGTTCTTGCGGAATTTGGTGCAGATGTCACCTTAATAGAAAGAGGTCAGCCTGTTGAACAGAGGGGGCGCGACATTGGTGCTTTGGCTGTTCGTCGGATCTTGCAGACAGAGAGCAATTTCTGCTTTGGAGAGGGTGGTGCAGGTACCTGGAGTGATGGAAAGCTGGTGACTAGGATTGGAAGAAACAGTGGGAGTGTTTTGGCGGTTATGGAAACGTTAGTTCAATTTGGAGCTCCAGAAGGAATTTTGGTTAACGGAAAGCCTCATTTGGGAACAGATAAATTGGTTCCACTACTTCGCAATTTTCGGCAGCATCTACTAAAGCTGGGTGTGACCATCAAGTTTGGCACAAGGGTTGATGATCTCCTTGTAGATAATGAACAAGTTGTAGGTGTCAGAGTTTCTGATTCAGTCGACAGGTTACAGTCTAGTAGCAAGAAATGGGAGTACAATGCTGTTGTTCTGGCTGTTGGACATTCTGCACGGGATCTTTATCAAACACTTCTATCTCATAACATTGAGTTGATCCCAAAAGATTTTGCTGTTGGCTTGCGGATTGAGCATCCTCAAGAAGTTGTAAACAGCTTACAGTATTCTGGATTGGCAACTGAGGTTTGCAGAGGACGCGGTAAAGTACCAGTGGCAGATTACAAGGTTGCAAAGTATGTAAGTGGAGAGAACAGGGTTGAACCTTTGCATGCAACAAGTCGAAGCTGCTATTCCTTCTGTATGTGTCCTGGTGGTCAGGTTGTTCTTACAAGTACAAATCCATCTGAAATCTGTGTCAATGGCATGTCATTTTCTCGACGTGACTCAAAGTGGGCAAATGCTGCACTTGTTGTGACCGTCTCAACAAAAGATTTTGGTGCATTGAATCTCCATGGACCTCTAGCAGGGGTTGAGTTTCAGAGAGAATTTGAGCAAAGAGCAGCAAGAATGGGAGGTGGAAATTTTGTGGTACCTGTGCAGACAGTTACAGATTTTATGGACAATAAATTATCAGTAACATCTGTGCCACAGTCAAGTTATCGATTAGGAGTGAAGGCAGCAAATCTTCACAAGTTATTTCCTGATTATGTAACAGAGACTTTGCAGCAGTCAATCTCAGCATTTGACAAAGAGATTTTCGTTTTCTTGAAATTTGGAACCTTCAGTTACCGGGCTTTATCTGCGAAGAGGCCCTTCTTCATGGAGTGGAGGACCAGTTCTCCCATCCAGATTCCCCGCAGCGTTGAAACTTATGAGAGCACATCGTTGAAAGGTCTCTACCCTATTGGTGAAGGAGCAGGTTATGCCGGAGGGATTGTAAGTGCAGCTGTGGATGGTATGCATGCTGGTTTTGCAGTGGCAAAAAATTTTGGATTGTGTAATGATGGCATAGAGTCAATTTTGGGCAAGGCTCGGAGTGCTGGATTTTTGGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_2 FAD_binding_3 Pyr_redox Pyr_redox_2

Protein Analysis

710

Amino Acids

77.78

Weight (kDa)

9.39

Isoelectric Point (pI)

38.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 220 - 399 8.5e-07 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 220 - 269 7.5e-06 Pyridine nucleotide-disulphide oxidoreductase
FAD-depend_C PF21688 414 - 614 8.9e-60 FAD-dependent protein, C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30720
fragaria_vesca FvH4_1g00650 FvH4_1g00650
malus_domestica MD02G1005300.v1.1
prunus_persica Prupe.7G265700_v2.0.a1 Prupe.7G265700_v2.0.a1
pyrus_communis pycom02g00410
rosa_chinensis RchiOBHm_Chr2g0085271
rosa_laevigata RLG00000015673
rosa_multiflora Rmu_sc0008510.1_g000007
rosa_roxburghii Rroxscaffold_2G00155500
rosa_rugosa Rorug01G0458800 Rorug01G0458900
rosa_samantha Rh2AG007500 Rh2BG008100 Rh2CG008900
rosa_wichuraiana Rw2G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 836, 1982
Acc65I GGTACC 2 cut(s) 848, 1663
AccB1I GGYRCC 2 cut(s) 848, 1663
AccI GTMKAC 2 cut(s) 246, 1119
AccII CGCG 2 cut(s) 774, 1332
AciI CCGC 8 cut(s) 49, 114, 665, 713, 908, 1258, 1332, 1930
AclI AACGTT 1 cut(s) 920
AclWI GGATC 4 cut(s) 634, 809, 1200, 1225
AcsI RAATTY 8 cut(s) 404, 635, 716, 948, 1619, 1654, 1844, 2059
AcuI CTGAAG 1 cut(s) 1840
AcyI GRCGYC 2 cut(s) 91, 276
AfaI GTAC 7 cut(s) 632, 850, 1154, 1341, 1468, 1665, 2129
AfiI CCNNNNNNNGG 5 cut(s) 262, 363, 494, 974, 1598
AjiI CACGTC 1 cut(s) 1514
AjnI CCWGG 3 cut(s) 521, 851, 1441
AloI GAACNNNNNNTCC 2 cut(s) 1894, 1926
AluBI AGCT 9 cut(s) 232, 288, 439, 868, 938, 1030, 1290, 1419, 2021
AluI AGCT 9 cut(s) 232, 288, 439, 868, 938, 1030, 1290, 1419, 2021
Alw21I GWGCWC 4 cut(s) 162, 315, 940, 1955
Alw26I GTCTC 5 cut(s) 283, 624, 1558, 1784, 1973
AlwI GGATC 4 cut(s) 634, 809, 1200, 1225
AlwNI CAGNNNCTG 1 cut(s) 587
Ama87I CYCGRG 1 cut(s) 314
AoxI GGCC 2 cut(s) 348, 1883
ApoI RAATTY 8 cut(s) 404, 635, 716, 948, 1619, 1654, 1844, 2059
Asp718I GGTACC 2 cut(s) 848, 1663
AspLEI GCGC 1 cut(s) 774
AspS9I GGNCC 5 cut(s) 123, 680, 1589, 1884, 1905
AsuC2I CCSGG 2 cut(s) 258, 1865
AsuHPI GGTGA 4 cut(s) 346, 724, 883, 1994
AvaI CYCGRG 1 cut(s) 314
AvaII GGWCC 4 cut(s) 123, 680, 1589, 1905
AxyI CCTNAGG 1 cut(s) 489
BanI GGYRCC 2 cut(s) 848, 1663
BanII GRGCYC 1 cut(s) 940
BauI CACGAG 1 cut(s) 456
Bbv12I GWGCWC 4 cut(s) 162, 315, 940, 1955
BccI CCATC 8 cut(s) 48, 691, 854, 1049, 1483, 1925, 2021, 2072
BceAI ACGGC 1 cut(s) 325
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciT130I CCWGG 3 cut(s) 523, 853, 1443
BcnI CCSGG 2 cut(s) 258, 1865
BcoDI GTCTC 5 cut(s) 283, 624, 1558, 1784, 1973
BfaI CTAG 6 cut(s) 75, 648, 876, 1134, 1595, 2131
BfuAI ACCTGC 2 cut(s) 836, 1982
BglII AGATCT 1 cut(s) 105
BmcAI AGTACT 1 cut(s) 2129
Bme1390I CCNGG 5 cut(s) 258, 523, 853, 1443, 1865
Bme18I GGWCC 4 cut(s) 123, 680, 1589, 1905
BmeT110I CYCGRG 1 cut(s) 314
BmgBI CACGTC 1 cut(s) 1514
BmgT120I GGNCC 5 cut(s) 123, 680, 1589, 1884, 1905
BmiI GGNNCC 5 cut(s) 486, 850, 993, 1665, 1852
BmrFI CCNGG 5 cut(s) 258, 523, 853, 1443, 1865
BmsI GCATC 4 cut(s) 367, 580, 1027, 1276
BpmI CTGGAG 5 cut(s) 94, 552, 609, 874, 924
BpuEI CTTGAG 5 cut(s) 86, 146, 501, 606, 1257
BpuMI CCSGG 2 cut(s) 258, 1865
Bsa29I ATCGAT 1 cut(s) 1735
BsaAI YACGTR 1 cut(s) 415
BsaBI GATNNNNATC 1 cut(s) 1197
BsaHI GRCGYC 2 cut(s) 91, 276
BsaI GGTCTC 2 cut(s) 624, 1973
BsaJI CCNNGG 4 cut(s) 256, 271, 357, 1585
BsaWI WCCGGW 2 cut(s) 241, 322
BsaXI ACNNNNNCTCC 6 cut(s) 1894, 1897, 1924, 1927, 1995, 2025
Bsc4I CCNNNNNNNGG 5 cut(s) 262, 363, 494, 974, 1598
Bse118I RCCGGY 1 cut(s) 643
Bse1I ACTGG 3 cut(s) 543, 1343, 1908
Bse21I CCTNAGG 1 cut(s) 489
Bse8I GATNNNNATC 1 cut(s) 1197
BseBI CCWGG 3 cut(s) 523, 853, 1443
BseCI ATCGAT 1 cut(s) 1735
BseDI CCNNGG 4 cut(s) 256, 271, 357, 1585
BseGI GGATG 5 cut(s) 82, 433, 1267, 1917, 2032
BseJI GATNNNNATC 1 cut(s) 1197
BseLI CCNNNNNNNGG 5 cut(s) 262, 363, 494, 974, 1598
BseMII CTCAG 2 cut(s) 1305, 1824
BseNI ACTGG 3 cut(s) 543, 1343, 1908
BseRI GAGGAG 1 cut(s) 35
BseYI CCCAGC 1 cut(s) 1030
BsgI GTGCAG 6 cut(s) 744, 864, 1170, 1521, 1691, 2037
Bsh1236I CGCG 2 cut(s) 774, 1332
Bsh1285I CGRYCG 2 cut(s) 246, 250
BshFI GGCC 2 cut(s) 350, 1885
BshNI GGYRCC 2 cut(s) 848, 1663
BshVI ATCGAT 1 cut(s) 1735
BsiEI CGRYCG 2 cut(s) 246, 250
BsiHKAI GWGCWC 4 cut(s) 162, 315, 940, 1955
BsiHKCI CYCGRG 1 cut(s) 314
BsiSI CCGG 7 cut(s) 126, 242, 258, 323, 644, 1864, 2001
BslFI GGGAC 1 cut(s) 479
BslI CCNNNNNNNGG 5 cut(s) 262, 363, 494, 974, 1598
BsmAI GTCTC 5 cut(s) 283, 624, 1558, 1784, 1973
BsmBI CGTCTC 2 cut(s) 283, 1558
BsmFI GGGAC 1 cut(s) 479
BsnI GGCC 2 cut(s) 350, 1885
Bso31I GGTCTC 2 cut(s) 624, 1973
BsoBI CYCGRG 1 cut(s) 314
Bsp1286I GDGCHC 4 cut(s) 162, 315, 940, 1955
Bsp143I GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
Bsp19I CCATGG 1 cut(s) 1585
BspACI CCGC 8 cut(s) 49, 114, 665, 713, 908, 1258, 1332, 1930
BspANI GGCC 2 cut(s) 350, 1885
BspCNI CTCAG 2 cut(s) 1306, 1823
BspDI ATCGAT 1 cut(s) 1735
BspFNI CGCG 2 cut(s) 774, 1332
BspLI GGNNCC 5 cut(s) 486, 850, 993, 1665, 1852
BspMI ACCTGC 2 cut(s) 836, 1982
BspPI GGATC 4 cut(s) 634, 809, 1200, 1225
BspT107I GGYRCC 2 cut(s) 848, 1663
BspTNI GGTCTC 2 cut(s) 624, 1973
BsrFI RCCGGY 1 cut(s) 643
BsrI ACTGG 3 cut(s) 543, 1343, 1908
BssAI RCCGGY 1 cut(s) 643
BssECI CCNNGG 4 cut(s) 256, 271, 357, 1585
BssMI GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
BssNI GRCGYC 2 cut(s) 91, 276
BssSI CACGAG 1 cut(s) 456
BssT1I CCWWGG 1 cut(s) 1585
Bst2BI CACGAG 1 cut(s) 456
Bst2UI CCWGG 3 cut(s) 523, 853, 1443
Bst4CI ACNGT 8 cut(s) 251, 358, 893, 1131, 1296, 1552, 1678, 1725
Bst6I CTCTTC 2 cut(s) 380, 1874
BstACI GRCGYC 2 cut(s) 91, 276
BstAPI GCANNNNNTGC 3 cut(s) 825, 1535, 1997
BstBAI YACGTR 1 cut(s) 415
BstC8I GCNNGC 6 cut(s) 131, 234, 290, 1256, 1404, 2037
BstDEI CTNAG 3 cut(s) 489, 1314, 1810
BstDSI CCRYGG 2 cut(s) 357, 1585
BstENI CCTNNNNNAGG 1 cut(s) 1596
BstF5I GGATG 5 cut(s) 82, 433, 1267, 1917, 2032
BstFNI CGCG 2 cut(s) 774, 1332
BstHHI GCGC 1 cut(s) 774
BstKTI GATC 6 cut(s) 108, 629, 804, 1069, 1195, 1233
BstMAI GTCTC 5 cut(s) 283, 624, 1558, 1784, 1973
BstMBI GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
BstMCI CGRYCG 2 cut(s) 246, 250
BstMWI GCNNNNNNNGC 9 cut(s) 229, 825, 1264, 1329, 1535, 1874, 1997, 2045, 2111
BstNI CCWGG 3 cut(s) 523, 853, 1443
BstNSI RCATGY 4 cut(s) 514, 1406, 1501, 2039
BstSCI CCNGG 5 cut(s) 256, 521, 851, 1441, 1863
BstSNI TACGTA 1 cut(s) 415
BstUI CGCG 2 cut(s) 774, 1332
BstX2I RGATCY 3 cut(s) 105, 801, 1192
BstYI RGATCY 3 cut(s) 105, 801, 1192
Bsu15I ATCGAT 1 cut(s) 1735
Bsu36I CCTNAGG 1 cut(s) 489
BsuRI GGCC 2 cut(s) 350, 1885
BsuTUI ATCGAT 1 cut(s) 1735
BtgI CCRYGG 2 cut(s) 357, 1585
BtrI CACGTC 1 cut(s) 1514
BtsCI GGATG 5 cut(s) 82, 433, 1267, 1917, 2032
BtsI GCAGTG 2 cut(s) 682, 2055
BtsIMutI CAGTG 4 cut(s) 682, 898, 1350, 2055
BveI ACCTGC 2 cut(s) 836, 1982
Cac8I GCNNGC 6 cut(s) 131, 234, 290, 1256, 1404, 2037
CaiI CAGNNNCTG 1 cut(s) 587
CfoI GCGC 1 cut(s) 774
Cfr10I RCCGGY 1 cut(s) 643
Cfr13I GGNCC 5 cut(s) 123, 680, 1589, 1884, 1905
ClaI ATCGAT 1 cut(s) 1735
CseI GACGC 3 cut(s) 99, 265, 1338
Csp6I GTAC 7 cut(s) 631, 849, 1153, 1340, 1467, 1664, 2128
CviAII CATG 7 cut(s) 511, 554, 1403, 1498, 1586, 1894, 2036
CviQI GTAC 7 cut(s) 631, 849, 1153, 1340, 1467, 1664, 2128
DdeI CTNAG 3 cut(s) 489, 1314, 1810
DpnI GATC 6 cut(s) 107, 628, 803, 1068, 1194, 1232
DpnII GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
Eam1104I CTCTTC 2 cut(s) 380, 1874
EarI CTCTTC 2 cut(s) 380, 1874
EciI GGCGGA 1 cut(s) 38
Ecl136II GAGCTC 1 cut(s) 938
Eco105I TACGTA 1 cut(s) 415
Eco130I CCWWGG 1 cut(s) 1585
Eco147I AGGCCT 1 cut(s) 350
Eco24I GRGCYC 1 cut(s) 940
Eco31I GGTCTC 2 cut(s) 624, 1973
Eco32I GATATC 2 cut(s) 181, 528
Eco47I GGWCC 4 cut(s) 123, 680, 1589, 1905
Eco53kI GAGCTC 1 cut(s) 938
Eco57I CTGAAG 1 cut(s) 1840
Eco81I CCTNAGG 1 cut(s) 489
Eco88I CYCGRG 1 cut(s) 314
EcoICRI GAGCTC 1 cut(s) 938
EcoNI CCTNNNNNAGG 1 cut(s) 1596
EcoO109I RGGNCCY 1 cut(s) 1884
EcoRII CCWGG 3 cut(s) 521, 851, 1441
EcoRV GATATC 2 cut(s) 181, 528
EcoT14I CCWWGG 1 cut(s) 1585
EcoT22I ATGCAT 1 cut(s) 2037
EcoT38I GRGCYC 1 cut(s) 940
ErhI CCWWGG 1 cut(s) 1585
Esp3I CGTCTC 2 cut(s) 283, 1558
FaeI CATG 7 cut(s) 514, 557, 1406, 1501, 1589, 1897, 2039
FalI AAGNNNNNCTT 2 cut(s) 1871, 1903
FaqI GGGAC 1 cut(s) 479
FatI CATG 7 cut(s) 510, 553, 1402, 1497, 1585, 1893, 2035
FauI CCCGC 1 cut(s) 1937
FblI GTMKAC 2 cut(s) 246, 1119
FokI GGATG 5 cut(s) 69, 440, 1254, 1904, 2039
FriOI GRGCYC 1 cut(s) 940
FspBI CTAG 6 cut(s) 75, 648, 876, 1134, 1595, 2131
GlaI GCGC 1 cut(s) 773
GsaI CCCAGC 1 cut(s) 1034
GsuI CTGGAG 5 cut(s) 94, 552, 609, 874, 924
HaeIII GGCC 2 cut(s) 350, 1885
HapII CCGG 7 cut(s) 126, 242, 258, 323, 644, 1864, 2001
HgaI GACGC 3 cut(s) 99, 265, 1338
HhaI GCGC 1 cut(s) 774
Hin1I GRCGYC 2 cut(s) 91, 276
Hin1II CATG 7 cut(s) 514, 557, 1406, 1501, 1589, 1897, 2039
Hin6I GCGC 1 cut(s) 772
HinP1I GCGC 1 cut(s) 772
HincII GTYRAC 4 cut(s) 247, 433, 958, 1120
HindII GTYRAC 4 cut(s) 247, 433, 958, 1120
HinfI GANTC 5 cut(s) 1112, 1517, 1579, 1924, 2087
HpaI GTTAAC 1 cut(s) 958
HpaII CCGG 7 cut(s) 126, 242, 258, 323, 644, 1864, 2001
HphI GGTGA 4 cut(s) 346, 724, 883, 1994
Hpy166II GTNNAC 7 cut(s) 247, 412, 433, 680, 958, 1120, 1285
Hpy8I GTNNAC 7 cut(s) 247, 412, 433, 680, 958, 1120, 1285
Hpy99I CGWCG 6 cut(s) 93, 148, 188, 346, 801, 1515
HpyAV CCTTC 9 cut(s) 20, 148, 361, 938, 1438, 1741, 1864, 1897, 1979
HpyCH4III ACNGT 8 cut(s) 251, 358, 893, 1131, 1296, 1552, 1678, 1725
HpyCH4IV ACGT 4 cut(s) 192, 414, 920, 1513
HpyF10VI GCNNNNNNNGC 9 cut(s) 229, 825, 1264, 1329, 1535, 1874, 1997, 2045, 2111
HpyF3I CTNAG 3 cut(s) 489, 1314, 1810
HpySE526I ACGT 4 cut(s) 192, 414, 920, 1513
Hsp92I GRCGYC 2 cut(s) 91, 276
Hsp92II CATG 7 cut(s) 514, 557, 1406, 1501, 1589, 1897, 2039
HspAI GCGC 1 cut(s) 772
KpnI GGTACC 2 cut(s) 852, 1667
KspAI GTTAAC 1 cut(s) 958
Kzo9I GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
LmnI GCTCC 4 cut(s) 484, 935, 943, 1988
LweI GCATC 4 cut(s) 367, 580, 1027, 1276
MaeI CTAG 6 cut(s) 75, 648, 876, 1134, 1595, 2131
MaeII ACGT 4 cut(s) 192, 414, 920, 1513
MalI GATC 6 cut(s) 107, 628, 803, 1068, 1194, 1232
MboI GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
MboII GAAGA 6 cut(s) 308, 397, 897, 1751, 1882, 1891
MflI RGATCY 3 cut(s) 105, 801, 1192
MhlI GDGCHC 4 cut(s) 162, 315, 940, 1955
MlyI GAGTC 2 cut(s) 1511, 2096
MmeI TCCRAC 5 cut(s) 126, 324, 462, 779, 1156
Mph1103I ATGCAT 1 cut(s) 2037
MseI TTAA 2 cut(s) 737, 957
MspA1I CMGCKG 2 cut(s) 114, 2021
MspI CCGG 7 cut(s) 126, 242, 258, 323, 644, 1864, 2001
MspR9I CCNGG 5 cut(s) 258, 523, 853, 1443, 1865
MvaI CCWGG 3 cut(s) 523, 853, 1443
MvnI CGCG 2 cut(s) 774, 1332
MwoI GCNNNNNNNGC 9 cut(s) 229, 825, 1264, 1329, 1535, 1874, 1997, 2045, 2111
NciI CCSGG 2 cut(s) 258, 1865
NcoI CCATGG 1 cut(s) 1585
NdeII GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
NlaIII CATG 7 cut(s) 514, 557, 1406, 1501, 1589, 1897, 2039
NlaIV GGNNCC 5 cut(s) 486, 850, 993, 1665, 1852
NmeAIII GCCGAG 1 cut(s) 252
NmuCI GTSAC 7 cut(s) 3, 251, 730, 871, 1036, 1514, 1546
NsiI ATGCAT 1 cut(s) 2037
NspI RCATGY 4 cut(s) 514, 1406, 1501, 2039
PaeI GCATGC 2 cut(s) 1406, 2039
PaeR7I CTCGAG 1 cut(s) 314
PceI AGGCCT 1 cut(s) 350
PcsI WCGNNNNNNNCGW 1 cut(s) 189
PfeI GAWTC 3 cut(s) 1112, 1579, 1924
PleI GAGTC 2 cut(s) 1511, 2095
PpsI GAGTC 2 cut(s) 1511, 2095
Ppu21I YACGTR 1 cut(s) 415
Psp124BI GAGCTC 1 cut(s) 940
Psp1406I AACGTT 1 cut(s) 920
Psp6I CCWGG 3 cut(s) 521, 851, 1441
PspFI CCCAGC 1 cut(s) 1030
PspGI CCWGG 3 cut(s) 521, 851, 1441
PspN4I GGNNCC 5 cut(s) 486, 850, 993, 1665, 1852
PspPI GGNCC 5 cut(s) 123, 680, 1589, 1884, 1905
PspXI VCTCGAGB 1 cut(s) 314
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 3 cut(s) 105, 801, 1192
PvuII CAGCTG 1 cut(s) 2021
RsaI GTAC 7 cut(s) 632, 850, 1154, 1341, 1468, 1665, 2129
RsaNI GTAC 7 cut(s) 631, 849, 1153, 1340, 1467, 1664, 2128
SacI GAGCTC 1 cut(s) 940
SalI GTCGAC 2 cut(s) 245, 1118
SaqAI TTAA 2 cut(s) 737, 957
Sau3AI GATC 6 cut(s) 105, 626, 801, 1066, 1192, 1230
Sau96I GGNCC 5 cut(s) 123, 680, 1589, 1884, 1905
ScaI AGTACT 1 cut(s) 2129
SchI GAGTC 2 cut(s) 1511, 2096
ScrFI CCNGG 5 cut(s) 258, 523, 853, 1443, 1865
SduI GDGCHC 4 cut(s) 162, 315, 940, 1955
SfaNI GCATC 4 cut(s) 367, 580, 1027, 1276
Sfr274I CTCGAG 1 cut(s) 314
SinI GGWCC 4 cut(s) 123, 680, 1589, 1905
SlaI CTCGAG 1 cut(s) 314
SmlI CTYRAG 6 cut(s) 101, 161, 314, 516, 621, 1272
SmoI CTYRAG 6 cut(s) 101, 161, 314, 516, 621, 1272
SnaBI TACGTA 1 cut(s) 415
SphI GCATGC 2 cut(s) 1406, 2039
SseBI AGGCCT 1 cut(s) 350
SsiI CCGC 8 cut(s) 49, 114, 665, 713, 908, 1258, 1332, 1930
SspMI CTAG 6 cut(s) 75, 648, 876, 1134, 1595, 2131
SstI GAGCTC 1 cut(s) 940
StuI AGGCCT 1 cut(s) 350
StyD4I CCNGG 5 cut(s) 256, 521, 851, 1441, 1863
StyI CCWWGG 1 cut(s) 1585
TaaI ACNGT 8 cut(s) 251, 358, 893, 1131, 1296, 1552, 1678, 1725
TaiI ACGT 4 cut(s) 195, 417, 923, 1516
TaqI TCGA 7 cut(s) 88, 246, 315, 1119, 1414, 1510, 1735
TatI WGTACW 3 cut(s) 1152, 1466, 2127
TauI GCSGC 1 cut(s) 117
TfiI GAWTC 3 cut(s) 1112, 1579, 1924
Tru1I TTAA 2 cut(s) 737, 957
Tru9I TTAA 2 cut(s) 737, 957
TscAI CASTG 4 cut(s) 682, 898, 1350, 2055
TseFI GTSAC 7 cut(s) 3, 251, 730, 871, 1036, 1514, 1546
Tsp45I GTSAC 7 cut(s) 3, 251, 730, 871, 1036, 1514, 1546
TspDTI ATGAA 3 cut(s) 460, 1098, 1882
TspGWI ACGGA 1 cut(s) 975
TspRI CASTG 4 cut(s) 682, 898, 1350, 2055
VpaK11BI GGWCC 4 cut(s) 123, 680, 1589, 1905
XagI CCTNNNNNAGG 1 cut(s) 1596
XapI RAATTY 8 cut(s) 404, 635, 716, 948, 1619, 1654, 1844, 2059
XceI RCATGY 4 cut(s) 514, 1406, 1501, 2039
XhoI CTCGAG 1 cut(s) 314
XmiI GTMKAC 2 cut(s) 246, 1119
XspI CTAG 6 cut(s) 75, 648, 876, 1134, 1595, 2131
ZrmI AGTACT 1 cut(s) 2129
Zsp2I ATGCAT 1 cut(s) 2037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.