pycom02g00410

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0014340
Physical Location & Seq
Forward (+)
249773 .. 254083
4311 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g00410.3

Sequence Viewer

Length: 2277 bp
ATGTTGATCAACGATTTTTTTTGGGCTTGGCCTTTTCCCTATTGGGCCCTGAGACAAAGATTTGCGACCCAAACGGCATGTCGCATCACTGGAGATTTGCCTCCTTATCCCAAACCAGAAGCTCGCTCACTCGTCAGGAAGCGCGAAACACTCCGGCCTCCACAGCTGAGTCGAAAAGCCATGTTCCTCCTTCCCTCCACCTCCAAGCTTTACTCTTTCCACCCTCCAAACTCTATCCCTGCGCGCGTACTCCCTTATCCTCGTCCCCAAACACTCCGAATCATCGGCGCCAAGAGGGCCGGCAAGCGGAGGTACCCGTCGGAGAAGAAGGAGCTGAAACTGAAGCATAAGGAAATCGCAGGGGACGTCAAGAACAAGTACGAGGGCATCTGGAGGCTGTCCAAGCTCGGCGTCTCCGTCGACAAGGACCCGGGAAAGGACTTTCTTGGCGTCTCCGATGGCTTGCTCGAACAGATTGCCAAAGTGCTCGAATTTCCGGTTCCTTCGATGCTGCCGACGGAGGCGTTCACGGTGGTTCGGAAATCTTTTGATGCAAGGAAGAGGCTGAAAGAACCGAAATTTGTATACATTGTGGAAATGGATGTCGACAAACTTCTGAGTCTCGAGCCTCGTGCTTGGGACTTCATATCTGAGTTGGAGCCGAAAGTTGGGCTAGTAGAACACATGCCTGAAGCTAGAAAATCTGGAGATTTGATCAATATAATACAGGACTTTAAGAAAGTCCATCAAGGTATAGTTTCAAGAGGAAGTGGCAATAACAGGAGCAATGGGTCTGAAGGATCACACGCGCATCCCACAGCTACAAAACCGAAAATTGCGGTGGTGGGTAGTGGACCATCAGGGCTGTTTGCCGCCCTTGTTCTTGCGGAATTTGGTGCAGATGTTACGTTACTAGAAAGAGGTCAGCCCGTTGAACAGAGGGGCCGCGACATTGGCGCTCTGATTGTCCGCCGGATGTTACAAACAGAAAGCAATTTTTGCTTTGGGGAGGGTGGTGCAGGTACCTGGAGTGATGGAAAGCTGGTGACAAGGATTGGAAGAAACAGTGGCAGTGTTTTGGCGGTTATGGAAACGTTAGTTCACTTTGGGGCTCCCGAAGGAATCTTGGTTGATGGAAAGCCTCATCTGGGAACAGATAGATTGGTTCCATTACTTCGCAATTTTCGGCAACATCTGCAACAGCTGGGTGTTACCATAAAGTTTGGGACTAGGGTAGACGATCTCCTTGTAGATAATGCACAAGTTGTAGGTGTCAAAGTTTCTGATTCAGCTGACAGACAGTCTAATAGTCAGAAATGGGAATATGATGCTGTTGTTCTAGCTGTTGGGCATTCTGCACGCGATTTTTACCAAACACTTCTATCTCATAACATAGACTTGATCCCGAAAGATTTTGCTGTTGGCTTGCGTATTGAGCATCCTCAAGAAGTAGTAAACAGTTTACAGTATTCTGGATTGGCAACTGAGGTTCGCAGAGGACGTGGTAAAGTGCCAGTGGCAGATTACAAGGTTGCCAAATATGTTAGTGGAAAGGATGGGGAAGAACCTTCAGGGGCAACAAGTCGTAGTTGCTATTCCTTTTGTATGTGTCCTGGTGGTCAGGTTGTTCTCACTAGTACAAATCCATCTGAAATCTGTATCAATGGCATGTCATTTTCTAAACGTGCGTCAAAGTGGGCAAATGCTGCACTTGTTGTAACAGTCTCAGCGAAAGATTTTGATGTATTGAATCTCCGTGGACCTCTTGCTGGGGTTGAGTTTCAGAGGGAATTTGAGCAAAGAGCAGCTAGAATGGGCGGGGGAAATTTTGTGGTGCCAGTGCAGACGGTTACGGATTTTATGGACAATAAGTTGTCAGTGACTTCTGTGCCACCATCAAGTTATCGATTAGGAGTGAAGGCAGCAAATCTCCATGAGATTTTCCCCATTCATATAACAGAGACGTTGCAGCACTCACTCTCTGTGTTTGACAAAGAGTTACCAGGTTTTATATCCAAGGAGGCTCTTCTTCATGGAGTGGAGACTAGGACTAGCTCACCCATCCAGATTCCCCGCGACAATGATACTTATGAGAGCACATCGTTGAAAGGTCTCTACCCTGTTGGTGAAGGAGCAGGTTATGCTGGAGGGATTGTGAGTGCAGCTGTGGATGGCATGTACGCTGGTTTTGCAGTGGCAAAGAATTTTGGTTTGTGTAATGAAGGCATCGAGTCAATTTTGGGCAAGGCTCGGAGTGCTGGATTTTTGGAGTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_2 FAD_binding_3 Pyr_redox Pyr_redox_2

Protein Analysis

759

Amino Acids

83.26

Weight (kDa)

9.26

Isoelectric Point (pI)

36.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30720
fragaria_vesca FvH4_1g00650 FvH4_1g00650
malus_domestica MD02G1005300.v1.1
prunus_persica Prupe.7G265700_v2.0.a1 Prupe.7G265700_v2.0.a1
pyrus_communis pycom02g00410
rosa_chinensis RchiOBHm_Chr2g0085271
rosa_laevigata RLG00000015673
rosa_multiflora Rmu_sc0008510.1_g000007
rosa_roxburghii Rroxscaffold_2G00155500
rosa_rugosa Rorug01G0458800 Rorug01G0458900
rosa_samantha Rh2AG007500 Rh2BG008100 Rh2CG008900
rosa_wichuraiana Rw2G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 369
Acc36I ACCTGC 2 cut(s) 1010, 2126
Acc65I GGTACC 2 cut(s) 312, 1022
AccB1I GGYRCC 4 cut(s) 287, 312, 1022, 1834
AccI GTMKAC 4 cut(s) 420, 585, 606, 1236
AccII CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
AciI CCGC 9 cut(s) 307, 839, 873, 887, 946, 970, 1082, 1818, 2074
AclI AACGTT 1 cut(s) 1094
AclWI GGATC 2 cut(s) 808, 1396
AcsI RAATTY 6 cut(s) 491, 578, 890, 1790, 1825, 2203
AcuI CTGAAG 4 cut(s) 362, 711, 816, 1554
AcyI GRCGYC 4 cut(s) 288, 366, 411, 450
AfaI GTAC 7 cut(s) 249, 314, 380, 1024, 1639, 2180, 2273
AfiI CCNNNNNNNGG 5 cut(s) 306, 436, 668, 1148, 1769
AgsI TTSAA 4 cut(s) 762, 935, 1750, 2107
AhdI GACNNNNNGTC 1 cut(s) 1300
AhlI ACTAGT 1 cut(s) 1634
AjiI CACGTC 1 cut(s) 1502
AjnI CCWGG 3 cut(s) 1025, 1612, 2002
Alw21I GWGCWC 2 cut(s) 489, 2099
Alw26I GTCTC 8 cut(s) 46, 418, 457, 626, 1729, 1955, 2036, 2117
AlwI GGATC 2 cut(s) 808, 1396
Ama87I CYCGRG 2 cut(s) 430, 623
AoxI GGCC 5 cut(s) 29, 45, 155, 297, 943
ApaI GGGCCC 1 cut(s) 49
ApeKI GCWGC 6 cut(s) 511, 1706, 1805, 1922, 1969, 2162
ApoI RAATTY 6 cut(s) 491, 578, 890, 1790, 1825, 2203
ArsI GACNNNNNNTTYG 2 cut(s) 64, 96
Asp718I GGTACC 2 cut(s) 312, 1022
AspLEI GCGC 6 cut(s) 144, 244, 246, 290, 811, 959
AspS9I GGNCC 7 cut(s) 45, 46, 297, 427, 854, 943, 1760
AsuC2I CCSGG 2 cut(s) 431, 432
AsuHPI GGTGA 3 cut(s) 1057, 2049, 2138
AvaI CYCGRG 2 cut(s) 430, 623
AvaII GGWCC 3 cut(s) 427, 854, 1760
BaeGI GKGCMC 1 cut(s) 49
BanI GGYRCC 4 cut(s) 287, 312, 1022, 1834
BanII GRGCYC 2 cut(s) 49, 1114
BauI CACGAG 1 cut(s) 630
Bbv12I GWGCWC 2 cut(s) 489, 2099
BbvI GCAGC 6 cut(s) 498, 1693, 1817, 1934, 1981, 2174
BceAI ACGGC 1 cut(s) 90
BcgI CGANNNNNNTGC 4 cut(s) 458, 492, 614, 648
BciT130I CCWGG 3 cut(s) 1027, 1614, 2004
BclI TGATCA 2 cut(s) 6, 714
BcnI CCSGG 2 cut(s) 431, 432
BcoDI GTCTC 8 cut(s) 46, 418, 457, 626, 1729, 1955, 2036, 2117
BcuI ACTAGT 1 cut(s) 1634
BfoI RGCGCY 2 cut(s) 291, 960
BfuAI ACCTGC 2 cut(s) 1010, 2126
BglI GCCNNNNNGGC 1 cut(s) 296
BisI GCNGC 8 cut(s) 512, 873, 946, 1707, 1806, 1923, 1970, 2163
BlsI GCNGC 8 cut(s) 513, 874, 947, 1708, 1807, 1924, 1971, 2164
BmcAI AGTACT 1 cut(s) 2273
Bme1390I CCNGG 5 cut(s) 431, 432, 1027, 1614, 2004
Bme18I GGWCC 3 cut(s) 427, 854, 1760
BmeRI GACNNNNNGTC 1 cut(s) 1300
BmeT110I CYCGRG 2 cut(s) 430, 623
BmgBI CACGTC 1 cut(s) 1502
BmgT120I GGNCC 7 cut(s) 45, 46, 297, 427, 854, 943, 1760
BmrFI CCNGG 5 cut(s) 431, 432, 1027, 1614, 2004
BmsI GCATC 8 cut(s) 93, 396, 498, 541, 820, 1318, 1447, 2235
BpmI CTGGAG 5 cut(s) 111, 412, 726, 1048, 2166
BpuEI CTTGAG 1 cut(s) 1428
BpuMI CCSGG 2 cut(s) 431, 432
Bsa29I ATCGAT 1 cut(s) 1906
BsaHI GRCGYC 4 cut(s) 288, 366, 411, 450
BsaI GGTCTC 1 cut(s) 2117
BsaJI CCNNGG 3 cut(s) 430, 1756, 2016
BsaWI WCCGGW 1 cut(s) 496
BsaXI ACNNNNNCTCC 2 cut(s) 2139, 2169
Bsc4I CCNNNNNNNGG 5 cut(s) 306, 436, 668, 1148, 1769
Bse118I RCCGGY 1 cut(s) 299
Bse1I ACTGG 3 cut(s) 94, 1514, 1838
Bse3DI GCAATG 1 cut(s) 793
BseBI CCWGG 3 cut(s) 1027, 1614, 2004
BseCI ATCGAT 1 cut(s) 1906
BseDI CCNNGG 3 cut(s) 430, 1756, 2016
BseGI GGATG 7 cut(s) 607, 811, 981, 1438, 1561, 2061, 2176
BseLI CCNNNNNNNGG 5 cut(s) 306, 436, 668, 1148, 1769
BseMI GCAATG 1 cut(s) 793
BseMII CTCAG 6 cut(s) 41, 158, 608, 642, 1476, 1740
BseNI ACTGG 3 cut(s) 94, 1514, 1838
BsePI GCGCGC 1 cut(s) 242
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 6 cut(s) 498, 1693, 1817, 1934, 1981, 2174
BseYI CCCAGC 2 cut(s) 1204, 1769
BsgI GTGCAG 6 cut(s) 918, 1038, 1341, 1692, 1862, 2181
Bsh1236I CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
BshFI GGCC 5 cut(s) 31, 47, 157, 299, 945
BshNI GGYRCC 4 cut(s) 287, 312, 1022, 1834
BshVI ATCGAT 1 cut(s) 1906
BsiHKAI GWGCWC 2 cut(s) 489, 2099
BsiHKCI CYCGRG 2 cut(s) 430, 623
BsiSI CCGG 5 cut(s) 154, 300, 431, 497, 973
BslFI GGGAC 4 cut(s) 249, 377, 653, 1240
BslI CCNNNNNNNGG 5 cut(s) 306, 436, 668, 1148, 1769
BsmAI GTCTC 8 cut(s) 46, 418, 457, 626, 1729, 1955, 2036, 2117
BsmBI CGTCTC 3 cut(s) 418, 457, 1955
BsmFI GGGAC 4 cut(s) 249, 377, 653, 1240
BsmI GAATGC 1 cut(s) 1351
BsnI GGCC 5 cut(s) 31, 47, 157, 299, 945
Bso31I GGTCTC 1 cut(s) 2117
BsoBI CYCGRG 2 cut(s) 430, 623
Bsp120I GGGCCC 1 cut(s) 45
Bsp1286I GDGCHC 4 cut(s) 49, 489, 1114, 2099
Bsp143I GATC 5 cut(s) 6, 714, 800, 1240, 1401
BspACI CCGC 9 cut(s) 307, 839, 873, 887, 946, 970, 1082, 1818, 2074
BspANI GGCC 5 cut(s) 31, 47, 157, 299, 945
BspCNI CTCAG 6 cut(s) 42, 159, 609, 643, 1477, 1739
BspDI ATCGAT 1 cut(s) 1906
BspFNI CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
BspMI ACCTGC 2 cut(s) 1010, 2126
BspPI GGATC 2 cut(s) 808, 1396
BspQI GCTCTTC 1 cut(s) 2031
BspT107I GGYRCC 4 cut(s) 287, 312, 1022, 1834
BspTNI GGTCTC 1 cut(s) 2117
BsrDI GCAATG 1 cut(s) 793
BsrFI RCCGGY 1 cut(s) 299
BsrI ACTGG 3 cut(s) 94, 1514, 1838
BssAI RCCGGY 1 cut(s) 299
BssECI CCNNGG 3 cut(s) 430, 1756, 2016
BssHII GCGCGC 1 cut(s) 242
BssMI GATC 5 cut(s) 6, 714, 800, 1240, 1401
BssNAI GTATAC 1 cut(s) 586
BssNI GRCGYC 4 cut(s) 288, 366, 411, 450
BssSI CACGAG 1 cut(s) 630
BssT1I CCWWGG 1 cut(s) 2016
Bst1107I GTATAC 1 cut(s) 586
Bst2BI CACGAG 1 cut(s) 630
Bst2UI CCWGG 3 cut(s) 1027, 1614, 2004
Bst4CI ACNGT 7 cut(s) 532, 1067, 1302, 1460, 1467, 1723, 1849
Bst6I CTCTTC 2 cut(s) 554, 2031
BstACI GRCGYC 4 cut(s) 288, 366, 411, 450
BstAPI GCANNNNNTGC 4 cut(s) 999, 1195, 1706, 2141
BstC8I GCNNGC 7 cut(s) 124, 244, 301, 305, 464, 1360, 1427
BstDEI CTNAG 6 cut(s) 50, 167, 617, 651, 1485, 1726
BstDSI CCRYGG 1 cut(s) 1756
BstF5I GGATG 7 cut(s) 607, 811, 981, 1438, 1561, 2061, 2176
BstFNI CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
BstH2I RGCGCY 2 cut(s) 291, 960
BstHHI GCGC 6 cut(s) 144, 244, 246, 290, 811, 959
BstKTI GATC 5 cut(s) 9, 717, 803, 1243, 1404
BstMAI GTCTC 8 cut(s) 46, 418, 457, 626, 1729, 1955, 2036, 2117
BstMBI GATC 5 cut(s) 6, 714, 800, 1240, 1401
BstNI CCWGG 3 cut(s) 1027, 1614, 2004
BstNSI RCATGY 4 cut(s) 81, 688, 1672, 2179
BstSCI CCNGG 5 cut(s) 429, 430, 1025, 1612, 2002
BstSLI GKGCMC 1 cut(s) 49
BstUI CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
BstV1I GCAGC 6 cut(s) 498, 1693, 1817, 1934, 1981, 2174
BstZ17I GTATAC 1 cut(s) 586
Bsu15I ATCGAT 1 cut(s) 1906
BsuRI GGCC 5 cut(s) 31, 47, 157, 299, 945
BsuTUI ATCGAT 1 cut(s) 1906
BtgI CCRYGG 1 cut(s) 1756
BtrI CACGTC 1 cut(s) 1502
BtsCI GGATG 7 cut(s) 607, 811, 981, 1438, 1561, 2061, 2176
BtsI GCAGTG 2 cut(s) 1078, 2199
BtsIMutI CAGTG 7 cut(s) 87, 1072, 1078, 1521, 1845, 1884, 2199
BveI ACCTGC 2 cut(s) 1010, 2126
Cac8I GCNNGC 7 cut(s) 124, 244, 301, 305, 464, 1360, 1427
CfoI GCGC 6 cut(s) 144, 244, 246, 290, 811, 959
Cfr10I RCCGGY 1 cut(s) 299
Cfr13I GGNCC 7 cut(s) 45, 46, 297, 427, 854, 943, 1760
Cfr9I CCCGGG 1 cut(s) 430
ClaI ATCGAT 1 cut(s) 1906
CseI GACGC 3 cut(s) 400, 439, 1677
CsiI ACCWGGT 1 cut(s) 2002
Csp6I GTAC 7 cut(s) 248, 313, 379, 1023, 1638, 2179, 2272
CviAII CATG 7 cut(s) 78, 181, 685, 1669, 1934, 2033, 2176
CviQI GTAC 7 cut(s) 248, 313, 379, 1023, 1638, 2179, 2272
DdeI CTNAG 6 cut(s) 50, 167, 617, 651, 1485, 1726
DinI GGCGCC 1 cut(s) 289
DpnI GATC 5 cut(s) 8, 716, 802, 1242, 1403
DpnII GATC 5 cut(s) 6, 714, 800, 1240, 1401
DriI GACNNNNNGTC 1 cut(s) 1300
Eam1104I CTCTTC 2 cut(s) 554, 2031
Eam1105I GACNNNNNGTC 1 cut(s) 1300
EarI CTCTTC 2 cut(s) 554, 2031
EciI GGCGGA 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 2016
Eco24I GRGCYC 2 cut(s) 49, 1114
Eco31I GGTCTC 1 cut(s) 2117
Eco47I GGWCC 3 cut(s) 427, 854, 1760
Eco57I CTGAAG 4 cut(s) 362, 711, 816, 1554
Eco88I CYCGRG 2 cut(s) 430, 623
EcoO109I RGGNCCY 2 cut(s) 46, 427
EcoRII CCWGG 3 cut(s) 1025, 1612, 2002
EcoT14I CCWWGG 1 cut(s) 2016
EcoT38I GRGCYC 2 cut(s) 49, 1114
EgeI GGCGCC 1 cut(s) 289
EheI GGCGCC 1 cut(s) 289
ErhI CCWWGG 1 cut(s) 2016
Esp3I CGTCTC 3 cut(s) 418, 457, 1955
FaeI CATG 7 cut(s) 81, 184, 688, 1672, 1937, 2036, 2179
FaqI GGGAC 4 cut(s) 249, 377, 653, 1240
FatI CATG 7 cut(s) 77, 180, 684, 1668, 1933, 2032, 2175
FauI CCCGC 2 cut(s) 1811, 2081
FbaI TGATCA 2 cut(s) 6, 714
FblI GTMKAC 4 cut(s) 420, 585, 606, 1236
Fnu4HI GCNGC 8 cut(s) 512, 873, 946, 1707, 1806, 1923, 1970, 2163
FokI GGATG 7 cut(s) 614, 798, 988, 1425, 1568, 2048, 2183
FriOI GRGCYC 2 cut(s) 49, 1114
Fsp4HI GCNGC 8 cut(s) 512, 873, 946, 1707, 1806, 1923, 1970, 2163
GlaI GCGC 6 cut(s) 143, 243, 245, 289, 810, 958
GluI GCNGC 8 cut(s) 512, 873, 946, 1707, 1806, 1923, 1970, 2163
GsaI CCCAGC 2 cut(s) 1208, 1773
GsuI CTGGAG 5 cut(s) 111, 412, 726, 1048, 2166
HaeII RGCGCY 2 cut(s) 291, 960
HaeIII GGCC 5 cut(s) 31, 47, 157, 299, 945
HapII CCGG 5 cut(s) 154, 300, 431, 497, 973
HgaI GACGC 3 cut(s) 400, 439, 1677
HhaI GCGC 6 cut(s) 144, 244, 246, 290, 811, 959
Hin1I GRCGYC 4 cut(s) 288, 366, 411, 450
Hin1II CATG 7 cut(s) 81, 184, 688, 1672, 1937, 2036, 2179
Hin6I GCGC 6 cut(s) 142, 242, 244, 288, 809, 957
HinP1I GCGC 6 cut(s) 142, 242, 244, 288, 809, 957
HincII GTYRAC 2 cut(s) 421, 607
HindII GTYRAC 2 cut(s) 421, 607
HindIII AAGCTT 1 cut(s) 206
HinfI GANTC 8 cut(s) 169, 279, 619, 1122, 1286, 1750, 2068, 2231
HpaII CCGG 5 cut(s) 154, 300, 431, 497, 973
HphI GGTGA 3 cut(s) 1057, 2049, 2138
Hpy99I CGWCG 3 cut(s) 322, 422, 520
HpyAV CCTTC 9 cut(s) 200, 322, 513, 791, 1112, 1578, 1912, 2123, 2216
HpyCH4III ACNGT 7 cut(s) 532, 1067, 1302, 1460, 1467, 1723, 1849
HpyCH4IV ACGT 6 cut(s) 366, 908, 1094, 1501, 1684, 1964
HpyF3I CTNAG 6 cut(s) 50, 167, 617, 651, 1485, 1726
HpySE526I ACGT 6 cut(s) 366, 908, 1094, 1501, 1684, 1964
Hsp92I GRCGYC 4 cut(s) 288, 366, 411, 450
Hsp92II CATG 7 cut(s) 81, 184, 688, 1672, 1937, 2036, 2179
HspAI GCGC 6 cut(s) 142, 242, 244, 288, 809, 957
KasI GGCGCC 1 cut(s) 287
KpnI GGTACC 2 cut(s) 316, 1026
KroI GCCGGC 1 cut(s) 299
KroNI GCCGGC 1 cut(s) 301
Ksp22I TGATCA 2 cut(s) 6, 714
Kzo9I GATC 5 cut(s) 6, 714, 800, 1240, 1401
LguI GCTCTTC 1 cut(s) 2031
LmnI GCTCC 5 cut(s) 331, 658, 783, 1117, 2132
Lsp1109I GCAGC 6 cut(s) 498, 1693, 1817, 1934, 1981, 2174
LweI GCATC 8 cut(s) 93, 396, 498, 541, 820, 1318, 1447, 2235
MabI ACCWGGT 1 cut(s) 2002
MaeII ACGT 6 cut(s) 366, 908, 1094, 1501, 1684, 1964
MaeIII GTNAC 9 cut(s) 904, 909, 978, 1045, 1210, 1717, 1849, 1879, 1998
MalI GATC 5 cut(s) 8, 716, 802, 1242, 1403
MboI GATC 5 cut(s) 6, 714, 800, 1240, 1401
MboII GAAGA 6 cut(s) 337, 571, 1071, 1574, 2018, 2021
MhlI GDGCHC 4 cut(s) 49, 489, 1114, 2099
Mly113I GGCGCC 1 cut(s) 288
MlyI GAGTC 3 cut(s) 178, 628, 2240
MmeI TCCRAC 2 cut(s) 300, 636
MroNI GCCGGC 1 cut(s) 299
MseI TTAA 1 cut(s) 735
MspA1I CMGCKG 4 cut(s) 166, 1204, 1292, 2165
MspI CCGG 5 cut(s) 154, 300, 431, 497, 973
MspR9I CCNGG 5 cut(s) 431, 432, 1027, 1614, 2004
Mva1269I GAATGC 1 cut(s) 1351
MvaI CCWGG 3 cut(s) 1027, 1614, 2004
MvnI CGCG 7 cut(s) 144, 244, 246, 809, 948, 1362, 2076
NaeI GCCGGC 1 cut(s) 301
NarI GGCGCC 1 cut(s) 288
NciI CCSGG 2 cut(s) 431, 432
NdeII GATC 5 cut(s) 6, 714, 800, 1240, 1401
NgoMIV GCCGGC 1 cut(s) 299
NlaIII CATG 7 cut(s) 81, 184, 688, 1672, 1937, 2036, 2179
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 2 cut(s) 1045, 1879
NspI RCATGY 4 cut(s) 81, 688, 1672, 2179
PaeR7I CTCGAG 1 cut(s) 623
PauI GCGCGC 1 cut(s) 242
PciSI GCTCTTC 1 cut(s) 2031
PcsI WCGNNNNNNNCGW 4 cut(s) 363, 414, 512, 1498
PctI GAATGC 1 cut(s) 1351
PdiI GCCGGC 1 cut(s) 301
PfeI GAWTC 5 cut(s) 279, 1122, 1286, 1750, 2068
PkrI GCNGC 8 cut(s) 513, 874, 947, 1708, 1807, 1924, 1971, 2164
PleI GAGTC 3 cut(s) 177, 627, 2239
PluTI GGCGCC 1 cut(s) 291
PpsI GAGTC 3 cut(s) 177, 627, 2239
PpuMI RGGWCCY 1 cut(s) 427
Psp1406I AACGTT 1 cut(s) 1094
Psp5II RGGWCCY 1 cut(s) 427
Psp6I CCWGG 3 cut(s) 1025, 1612, 2002
PspFI CCCAGC 2 cut(s) 1204, 1769
PspGI CCWGG 3 cut(s) 1025, 1612, 2002
PspOMI GGGCCC 1 cut(s) 45
PspPI GGNCC 7 cut(s) 45, 46, 297, 427, 854, 943, 1760
PspPPI RGGWCCY 1 cut(s) 427
PteI GCGCGC 1 cut(s) 242
PvuII CAGCTG 4 cut(s) 166, 1204, 1292, 2165
RsaI GTAC 7 cut(s) 249, 314, 380, 1024, 1639, 2180, 2273
RsaNI GTAC 7 cut(s) 248, 313, 379, 1023, 1638, 2179, 2272
SalI GTCGAC 2 cut(s) 419, 605
SapI GCTCTTC 1 cut(s) 2031
SaqAI TTAA 1 cut(s) 735
SatI GCNGC 8 cut(s) 512, 873, 946, 1707, 1806, 1923, 1970, 2163
Sau3AI GATC 5 cut(s) 6, 714, 800, 1240, 1401
Sau96I GGNCC 7 cut(s) 45, 46, 297, 427, 854, 943, 1760
ScaI AGTACT 1 cut(s) 2273
SchI GAGTC 3 cut(s) 178, 628, 2240
ScrFI CCNGG 5 cut(s) 431, 432, 1027, 1614, 2004
SduI GDGCHC 4 cut(s) 49, 489, 1114, 2099
SexAI ACCWGGT 1 cut(s) 2002
SfaNI GCATC 8 cut(s) 93, 396, 498, 541, 820, 1318, 1447, 2235
SfoI GGCGCC 1 cut(s) 289
Sfr274I CTCGAG 1 cut(s) 623
SinI GGWCC 3 cut(s) 427, 854, 1760
SlaI CTCGAG 1 cut(s) 623
SmaI CCCGGG 1 cut(s) 432
SmlI CTYRAG 2 cut(s) 623, 1443
SmoI CTYRAG 2 cut(s) 623, 1443
SpeI ACTAGT 1 cut(s) 1634
SsiI CCGC 9 cut(s) 307, 839, 873, 887, 946, 970, 1082, 1818, 2074
SspDI GGCGCC 1 cut(s) 287
StyD4I CCNGG 5 cut(s) 429, 430, 1025, 1612, 2002
StyI CCWWGG 1 cut(s) 2016
TaaI ACNGT 7 cut(s) 532, 1067, 1302, 1460, 1467, 1723, 1849
TaiI ACGT 6 cut(s) 369, 911, 1097, 1504, 1687, 1967
TaqI TCGA 9 cut(s) 172, 420, 468, 489, 506, 606, 624, 1906, 2229
TatI WGTACW 2 cut(s) 1637, 2271
TauI GCSGC 2 cut(s) 875, 948
TfiI GAWTC 5 cut(s) 279, 1122, 1286, 1750, 2068
Tru1I TTAA 1 cut(s) 735
Tru9I TTAA 1 cut(s) 735
TscAI CASTG 7 cut(s) 94, 1072, 1078, 1521, 1845, 1884, 2199
TseFI GTSAC 2 cut(s) 1045, 1879
TseI GCWGC 6 cut(s) 511, 1706, 1805, 1922, 1969, 2162
Tsp45I GTSAC 2 cut(s) 1045, 1879
TspDTI ATGAA 4 cut(s) 634, 1940, 2021, 2235
TspGWI ACGGA 4 cut(s) 406, 533, 1745, 1868
TspMI CCCGGG 1 cut(s) 430
TspRI CASTG 7 cut(s) 94, 1072, 1078, 1521, 1845, 1884, 2199
VpaK11BI GGWCC 3 cut(s) 427, 854, 1760
XapI RAATTY 6 cut(s) 491, 578, 890, 1790, 1825, 2203
XceI RCATGY 4 cut(s) 81, 688, 1672, 2179
XhoI CTCGAG 1 cut(s) 623
XmaI CCCGGG 1 cut(s) 430
XmiI GTMKAC 4 cut(s) 420, 585, 606, 1236
ZraI GACGTC 1 cut(s) 367
ZrmI AGTACT 1 cut(s) 2273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.