MD02G1005300.v1.1

FAD binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014340
Physical Location & Seq
Forward (+)
363327 .. 367195
3869 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1005300.v1.1.491

Sequence Viewer

Length: 1890 bp
ATGTCCCTCCTTCCCTCCACCTCCAAGCTTTGCTCTTTCCACCCTCCAAACTCTATCCCTACCCGCGTACTCCCTTATCCTCGTCCCCAAACACTCCGAATCCTCGGCGCCAAGAGGGCCGGCAAGCGGAGGTACCCGTCGGAGAAGAAGGAGCTGAAACTGAAGCATAAGGAAATCGCAGGGGACGTCAAGAACAAGTACGAGGGCATCTGGAGGCTGTCCAAGCTCGGCGTCTCCGTCGACAAGGACCCGGGAAAGGACTTTCTCGGCGTCTCCGATGGCTTGCTCGAACAGATTGCCAAAGTACTCGAATTTCCGGTTCCTTCGATGCTGCCGACGGAGGCGTTCACGGTGGTTCGGAAATCTTTTGATGCAAGGAAGAGGCTGAAAGAACCGAAATTCGTATACATTGTGGAAATGGATGTCGACAAACTTCTGAGTCTCGAGCCTCGTGCTTGGGACTTCATTTCTGAGTTGGAGCCGAAAGTTGGGCTAGTAGAACACATGCCTGAAGCTAGAAAATCTGGAGATTTGATCAATATAATACAGGACTTTAAGAAAGTCCATCAAGGTATAGTTTCAAGAGGAAGTGGCAATAACAGGAGCAATGGGTCTGAAGGATCACACACGCATCCCACAGCTAAAAAACCAAAAATTGCGGTGGTGGGTAGTGGACCATCAGGGCTGTTTGCCGCCCTTGTTCTTGCAGAATTTGGTGCAGATGTTACGTTACTAGAAAGAGGTCAGCCCGTTGAACAGAGGGGCCGCGACATTGGCGCTCTGGTTGTCCGCCGGATGTTACAAACAGAAAGCAATTTTTGCTTTGGGGAGGGTGGTGCAGGTACCTGGAGTGATGGAAAGCTGGTGACAAGGATTGGAAGAAACAGTGGCAGTGTTTTGGCGGTTATGGAAACGTTAGTTCATTTTGGGGCTCCCGAAGGAATCTTGGTTGACGGAAAGCCTCATCTGGGAACAGATAGATTGGTTCCATTACTTCGCAATTTTCGGCAACATCTGCAACAGCTGGGTGTTACCATAAAGTTTGGGACTAGGGTAGACGATCTCCTTGTAGATAACGCACAAGTTGTAGGTGTCAGAGTTTCTGATTCGGCTGACAATAGTCAGAAATGGGGATATGATGCTGTTGTTCTAGCTGTTGGGCATTCTGCACGCGATTTTTATCAAACACTTCTATCTCATAACATAGACTTGATCCCGAAAGATTTTGCTGTTGGCTTGCGTATTGAGCATCCTCAAGAAGTAGTAAACAGCTTACAGTATTCTGGATTGGCAACTGAGGTTCGCAGAGGACGTGGTAAAGTGCCAGTGGCAGATTACAAGGTTGCCAAATATGTTAGTGGAAAGGATGGGGAAGAACCTTCAGGGGCAACAAGTCGTAGTTGCTATTCCTTTTGTATGTGTCCTGGTGGTCAGGTTGTTCTCACTAGTACAAATCCATCTGAAATCTGTATCAATGGCATGTCATTTTCTAAACGTGCGTCGAAGTGGGCAAATGCTGCACTTGTTGTAACAGTCTCAGCGAAAGATTTTGATGTTTTGAATCTCTGTGGACCTCTTGCTGGGGTTGAGTTTCAGAGGGAATTTGAGCAAAGAGCAGCTAGAATGGGCGGGGGGAATTTTGTGGTGCCAGTGCAGACAGTTACGGATTTTATGGACAATAAGTTGTCAGTGACTTCTGTGCCACCATCAAGTTATCGATTAGGAGTGAAGGCAGCAAATCTCCATGAGATTTTCCCCATTCATATAACAGAGACGTTGCAGCATTCACTCTCTGTGTTTGACAAAGAGTTACCAGGTTTTATATCCAAGGAGGCCCTTCTTCACGGAGTGGAGGTAATCCCAGTATTTAATATTTGTTTTAACTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
FAD_binding_2 FAD_binding_3 Pyr_redox Pyr_redox_2

Protein Analysis

630

Amino Acids

68.9

Weight (kDa)

9.08

Isoelectric Point (pI)

34.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pyr_redox_2 PF07992 218 - 393 1.8e-07 Pyridine nucleotide-disulphide oxidoreductase
Pyr_redox PF00070 218 - 276 2.2e-06 Pyridine nucleotide-disulphide oxidoreductase
FAD-depend_C PF21688 408 - 618 6.4e-63 FAD-dependent protein, C-terminal domain-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014340)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G30720
fragaria_vesca FvH4_1g00650 FvH4_1g00650
malus_domestica MD02G1005300.v1.1
prunus_persica Prupe.7G265700_v2.0.a1 Prupe.7G265700_v2.0.a1
pyrus_communis pycom02g00410
rosa_chinensis RchiOBHm_Chr2g0085271
rosa_laevigata RLG00000015673
rosa_multiflora Rmu_sc0008510.1_g000007
rosa_roxburghii Rroxscaffold_2G00155500
rosa_rugosa Rorug01G0458800 Rorug01G0458900
rosa_samantha Rh2AG007500 Rh2BG008100 Rh2CG008900
rosa_wichuraiana Rw2G000710

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 189
Acc36I ACCTGC 1 cut(s) 830
Acc65I GGTACC 2 cut(s) 132, 842
AccB1I GGYRCC 4 cut(s) 107, 132, 842, 1645
AccI GTMKAC 4 cut(s) 240, 405, 426, 1056
AccII CGCG 3 cut(s) 66, 768, 1173
AciI CCGC 8 cut(s) 64, 127, 659, 693, 766, 790, 902, 1629
AclI AACGTT 1 cut(s) 914
AclWI GGATC 2 cut(s) 628, 1207
AcsI RAATTY 5 cut(s) 311, 398, 710, 1601, 1636
AcuI CTGAAG 4 cut(s) 182, 531, 636, 1365
AcyI GRCGYC 4 cut(s) 108, 186, 231, 270
AdeI CACNNNGTG 1 cut(s) 1849
AfaI GTAC 6 cut(s) 69, 134, 200, 306, 844, 1450
AfiI CCNNNNNNNGG 5 cut(s) 126, 256, 488, 968, 1580
AgsI TTSAA 3 cut(s) 582, 755, 1561
AhlI ACTAGT 1 cut(s) 1445
AjiI CACGTC 1 cut(s) 1313
AjnI CCWGG 3 cut(s) 845, 1423, 1813
Alw26I GTCTC 5 cut(s) 238, 277, 446, 1540, 1766
AlwI GGATC 2 cut(s) 628, 1207
Ama87I CYCGRG 2 cut(s) 250, 443
AoxI GGCC 3 cut(s) 117, 763, 1833
ApeKI GCWGC 5 cut(s) 331, 1517, 1616, 1733, 1780
ApoI RAATTY 5 cut(s) 311, 398, 710, 1601, 1636
Asp718I GGTACC 2 cut(s) 132, 842
AspLEI GCGC 2 cut(s) 110, 779
AspS9I GGNCC 6 cut(s) 117, 247, 674, 763, 1571, 1834
AsuC2I CCSGG 2 cut(s) 251, 252
AsuHPI GGTGA 1 cut(s) 877
AvaI CYCGRG 2 cut(s) 250, 443
AvaII GGWCC 3 cut(s) 247, 674, 1571
BanI GGYRCC 4 cut(s) 107, 132, 842, 1645
BanII GRGCYC 1 cut(s) 934
BauI CACGAG 1 cut(s) 450
BbvI GCAGC 5 cut(s) 318, 1504, 1628, 1745, 1792
BccI CCATC 7 cut(s) 272, 573, 685, 848, 1361, 1465, 1714
BcgI CGANNNNNNTGC 4 cut(s) 278, 312, 434, 468
BciT130I CCWGG 3 cut(s) 847, 1425, 1815
BclI TGATCA 1 cut(s) 534
BcnI CCSGG 2 cut(s) 251, 252
BcoDI GTCTC 5 cut(s) 238, 277, 446, 1540, 1766
BcuI ACTAGT 1 cut(s) 1445
BfaI CTAG 7 cut(s) 494, 516, 734, 1050, 1151, 1446, 1620
BfoI RGCGCY 2 cut(s) 111, 780
BfuAI ACCTGC 1 cut(s) 830
BglI GCCNNNNNGGC 1 cut(s) 116
BisI GCNGC 7 cut(s) 332, 693, 766, 1518, 1617, 1734, 1781
BlsI GCNGC 7 cut(s) 333, 694, 767, 1519, 1618, 1735, 1782
BmcAI AGTACT 1 cut(s) 306
Bme1390I CCNGG 5 cut(s) 251, 252, 847, 1425, 1815
Bme18I GGWCC 3 cut(s) 247, 674, 1571
BmeT110I CYCGRG 2 cut(s) 250, 443
BmgBI CACGTC 1 cut(s) 1313
BmgT120I GGNCC 6 cut(s) 117, 247, 674, 763, 1571, 1834
BmrFI CCNGG 5 cut(s) 251, 252, 847, 1425, 1815
BmrI ACTGGG 1 cut(s) 1856
BmsI GCATC 6 cut(s) 216, 318, 361, 640, 1129, 1258
BmuI ACTGGG 1 cut(s) 1856
BoxI GACNNNNGTC 1 cut(s) 1119
BpmI CTGGAG 3 cut(s) 232, 546, 868
BpuEI CTTGAG 1 cut(s) 1239
BpuMI CCSGG 2 cut(s) 251, 252
Bsa29I ATCGAT 1 cut(s) 1717
BsaBI GATNNNNATC 1 cut(s) 1179
BsaHI GRCGYC 4 cut(s) 108, 186, 231, 270
BsaJI CCNNGG 3 cut(s) 103, 250, 1827
BsaWI WCCGGW 1 cut(s) 316
BsaXI ACNNNNNCTCC 2 cut(s) 1839, 1869
Bsc4I CCNNNNNNNGG 5 cut(s) 126, 256, 488, 968, 1580
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 3 cut(s) 1325, 1649, 1862
Bse3DI GCAATG 1 cut(s) 613
Bse8I GATNNNNATC 1 cut(s) 1179
BseBI CCWGG 3 cut(s) 847, 1425, 1815
BseCI ATCGAT 1 cut(s) 1717
BseDI CCNNGG 3 cut(s) 103, 250, 1827
BseGI GGATG 5 cut(s) 427, 631, 801, 1249, 1372
BseJI GATNNNNATC 1 cut(s) 1179
BseLI CCNNNNNNNGG 5 cut(s) 126, 256, 488, 968, 1580
BseMI GCAATG 1 cut(s) 613
BseMII CTCAG 4 cut(s) 428, 462, 1287, 1551
BseNI ACTGG 3 cut(s) 1325, 1649, 1862
BseXI GCAGC 5 cut(s) 318, 1504, 1628, 1745, 1792
BseYI CCCAGC 2 cut(s) 1024, 1580
BsgI GTGCAG 5 cut(s) 738, 858, 1152, 1503, 1673
Bsh1236I CGCG 3 cut(s) 66, 768, 1173
BshFI GGCC 3 cut(s) 119, 765, 1835
BshNI GGYRCC 4 cut(s) 107, 132, 842, 1645
BshVI ATCGAT 1 cut(s) 1717
BsiHKCI CYCGRG 2 cut(s) 250, 443
BsiSI CCGG 4 cut(s) 120, 251, 317, 793
BslFI GGGAC 4 cut(s) 69, 197, 473, 1060
BslI CCNNNNNNNGG 5 cut(s) 126, 256, 488, 968, 1580
BsmAI GTCTC 5 cut(s) 238, 277, 446, 1540, 1766
BsmBI CGTCTC 3 cut(s) 238, 277, 1766
BsmFI GGGAC 4 cut(s) 69, 197, 473, 1060
BsmI GAATGC 2 cut(s) 1162, 1783
BsnI GGCC 3 cut(s) 119, 765, 1835
BsoBI CYCGRG 2 cut(s) 250, 443
Bsp1286I GDGCHC 1 cut(s) 934
Bsp143I GATC 4 cut(s) 534, 620, 1060, 1212
BspACI CCGC 8 cut(s) 64, 127, 659, 693, 766, 790, 902, 1629
BspANI GGCC 3 cut(s) 119, 765, 1835
BspCNI CTCAG 4 cut(s) 429, 463, 1288, 1550
BspDI ATCGAT 1 cut(s) 1717
BspFNI CGCG 3 cut(s) 66, 768, 1173
BspMI ACCTGC 1 cut(s) 830
BspPI GGATC 2 cut(s) 628, 1207
BspT107I GGYRCC 4 cut(s) 107, 132, 842, 1645
BsrDI GCAATG 1 cut(s) 613
BsrFI RCCGGY 1 cut(s) 119
BsrI ACTGG 3 cut(s) 1325, 1649, 1862
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 103, 250, 1827
BssMI GATC 4 cut(s) 534, 620, 1060, 1212
BssNAI GTATAC 1 cut(s) 406
BssNI GRCGYC 4 cut(s) 108, 186, 231, 270
BssSI CACGAG 1 cut(s) 450
BssT1I CCWWGG 1 cut(s) 1827
Bst1107I GTATAC 1 cut(s) 406
Bst2BI CACGAG 1 cut(s) 450
Bst2UI CCWGG 3 cut(s) 847, 1425, 1815
Bst4CI ACNGT 5 cut(s) 352, 887, 1278, 1534, 1660
Bst6I CTCTTC 1 cut(s) 374
BstACI GRCGYC 4 cut(s) 108, 186, 231, 270
BstAPI GCANNNNNTGC 3 cut(s) 819, 1015, 1517
BstC8I GCNNGC 5 cut(s) 121, 125, 284, 1171, 1238
BstDEI CTNAG 4 cut(s) 437, 471, 1296, 1537
BstF5I GGATG 5 cut(s) 427, 631, 801, 1249, 1372
BstFNI CGCG 3 cut(s) 66, 768, 1173
BstH2I RGCGCY 2 cut(s) 111, 780
BstHHI GCGC 2 cut(s) 110, 779
BstKTI GATC 4 cut(s) 537, 623, 1063, 1215
BstMAI GTCTC 5 cut(s) 238, 277, 446, 1540, 1766
BstMBI GATC 4 cut(s) 534, 620, 1060, 1212
BstMWI GCNNNNNNNGC 7 cut(s) 116, 223, 774, 819, 1015, 1246, 1517
BstNI CCWGG 3 cut(s) 847, 1425, 1815
BstNSI RCATGY 2 cut(s) 508, 1483
BstPAI GACNNNNGTC 1 cut(s) 1119
BstSCI CCNGG 5 cut(s) 249, 250, 845, 1423, 1813
BstUI CGCG 3 cut(s) 66, 768, 1173
BstV1I GCAGC 5 cut(s) 318, 1504, 1628, 1745, 1792
BstZ17I GTATAC 1 cut(s) 406
Bsu15I ATCGAT 1 cut(s) 1717
BsuRI GGCC 3 cut(s) 119, 765, 1835
BsuTUI ATCGAT 1 cut(s) 1717
BtrI CACGTC 1 cut(s) 1313
BtsCI GGATG 5 cut(s) 427, 631, 801, 1249, 1372
BtsI GCAGTG 1 cut(s) 898
BtsIMutI CAGTG 5 cut(s) 892, 898, 1332, 1656, 1695
BveI ACCTGC 1 cut(s) 830
Cac8I GCNNGC 5 cut(s) 121, 125, 284, 1171, 1238
CfoI GCGC 2 cut(s) 110, 779
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 6 cut(s) 117, 247, 674, 763, 1571, 1834
Cfr9I CCCGGG 1 cut(s) 250
ClaI ATCGAT 1 cut(s) 1717
CseI GACGC 3 cut(s) 220, 259, 1488
CsiI ACCWGGT 1 cut(s) 1813
Csp6I GTAC 6 cut(s) 68, 133, 199, 305, 843, 1449
CviAII CATG 3 cut(s) 505, 1480, 1745
CviQI GTAC 6 cut(s) 68, 133, 199, 305, 843, 1449
DdeI CTNAG 4 cut(s) 437, 471, 1296, 1537
DinI GGCGCC 1 cut(s) 109
DpnI GATC 4 cut(s) 536, 622, 1062, 1214
DpnII GATC 4 cut(s) 534, 620, 1060, 1212
DraIII CACNNNGTG 1 cut(s) 1849
Eam1104I CTCTTC 1 cut(s) 374
EarI CTCTTC 1 cut(s) 374
EciI GGCGGA 1 cut(s) 779
Eco130I CCWWGG 1 cut(s) 1827
Eco24I GRGCYC 1 cut(s) 934
Eco47I GGWCC 3 cut(s) 247, 674, 1571
Eco57I CTGAAG 4 cut(s) 182, 531, 636, 1365
Eco88I CYCGRG 2 cut(s) 250, 443
EcoO109I RGGNCCY 2 cut(s) 247, 1834
EcoRII CCWGG 3 cut(s) 845, 1423, 1813
EcoT14I CCWWGG 1 cut(s) 1827
EcoT38I GRGCYC 1 cut(s) 934
EgeI GGCGCC 1 cut(s) 109
EheI GGCGCC 1 cut(s) 109
ErhI CCWWGG 1 cut(s) 1827
Esp3I CGTCTC 3 cut(s) 238, 277, 1766
FaeI CATG 3 cut(s) 508, 1483, 1748
FaqI GGGAC 4 cut(s) 69, 197, 473, 1060
FatI CATG 3 cut(s) 504, 1479, 1744
FauI CCCGC 2 cut(s) 71, 1622
FbaI TGATCA 1 cut(s) 534
FblI GTMKAC 4 cut(s) 240, 405, 426, 1056
Fnu4HI GCNGC 7 cut(s) 332, 693, 766, 1518, 1617, 1734, 1781
FokI GGATG 5 cut(s) 434, 618, 808, 1236, 1379
FriOI GRGCYC 1 cut(s) 934
Fsp4HI GCNGC 7 cut(s) 332, 693, 766, 1518, 1617, 1734, 1781
FspBI CTAG 7 cut(s) 494, 516, 734, 1050, 1151, 1446, 1620
GlaI GCGC 2 cut(s) 109, 778
GluI GCNGC 7 cut(s) 332, 693, 766, 1518, 1617, 1734, 1781
GsaI CCCAGC 2 cut(s) 1028, 1584
GsuI CTGGAG 3 cut(s) 232, 546, 868
HaeII RGCGCY 2 cut(s) 111, 780
HaeIII GGCC 3 cut(s) 119, 765, 1835
HapII CCGG 4 cut(s) 120, 251, 317, 793
HgaI GACGC 3 cut(s) 220, 259, 1488
HhaI GCGC 2 cut(s) 110, 779
Hin1I GRCGYC 4 cut(s) 108, 186, 231, 270
Hin1II CATG 3 cut(s) 508, 1483, 1748
Hin6I GCGC 2 cut(s) 108, 777
HinP1I GCGC 2 cut(s) 108, 777
HincII GTYRAC 3 cut(s) 241, 427, 952
HindII GTYRAC 3 cut(s) 241, 427, 952
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 5 cut(s) 99, 439, 942, 1106, 1561
HpaII CCGG 4 cut(s) 120, 251, 317, 793
HphI GGTGA 1 cut(s) 877
Hpy166II GTNNAC 9 cut(s) 241, 348, 406, 427, 674, 952, 1057, 1267, 1571
Hpy188III TCNNGA 9 cut(s) 190, 211, 443, 525, 582, 935, 1216, 1256, 1284
Hpy8I GTNNAC 9 cut(s) 241, 348, 406, 427, 674, 952, 1057, 1267, 1571
Hpy99I CGWCG 4 cut(s) 142, 242, 340, 1504
HpyAV CCTTC 8 cut(s) 20, 142, 333, 611, 932, 1389, 1723, 1847
HpyCH4III ACNGT 5 cut(s) 352, 887, 1278, 1534, 1660
HpyCH4IV ACGT 6 cut(s) 186, 728, 914, 1312, 1495, 1775
HpyCH4V TGCA 9 cut(s) 374, 707, 719, 839, 1018, 1169, 1520, 1654, 1780
HpyF10VI GCNNNNNNNGC 7 cut(s) 116, 223, 774, 819, 1015, 1246, 1517
HpyF3I CTNAG 4 cut(s) 437, 471, 1296, 1537
HpySE526I ACGT 6 cut(s) 186, 728, 914, 1312, 1495, 1775
Hsp92I GRCGYC 4 cut(s) 108, 186, 231, 270
Hsp92II CATG 3 cut(s) 508, 1483, 1748
HspAI GCGC 2 cut(s) 108, 777
KasI GGCGCC 1 cut(s) 107
KpnI GGTACC 2 cut(s) 136, 846
KroI GCCGGC 1 cut(s) 119
KroNI GCCGGC 1 cut(s) 121
Ksp22I TGATCA 1 cut(s) 534
Kzo9I GATC 4 cut(s) 534, 620, 1060, 1212
LmnI GCTCC 4 cut(s) 151, 478, 603, 937
Lsp1109I GCAGC 5 cut(s) 318, 1504, 1628, 1745, 1792
LweI GCATC 6 cut(s) 216, 318, 361, 640, 1129, 1258
MabI ACCWGGT 1 cut(s) 1813
MaeI CTAG 7 cut(s) 494, 516, 734, 1050, 1151, 1446, 1620
MaeII ACGT 6 cut(s) 186, 728, 914, 1312, 1495, 1775
MaeIII GTNAC 9 cut(s) 724, 729, 798, 865, 1030, 1528, 1660, 1690, 1809
MalI GATC 4 cut(s) 536, 622, 1062, 1214
MboI GATC 4 cut(s) 534, 620, 1060, 1212
MboII GAAGA 5 cut(s) 157, 391, 891, 1385, 1832
MhlI GDGCHC 1 cut(s) 934
MluCI AATT 8 cut(s) 311, 398, 654, 710, 814, 1000, 1601, 1636
Mly113I GGCGCC 1 cut(s) 108
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 2 cut(s) 120, 456
MroNI GCCGGC 1 cut(s) 119
MseI TTAA 3 cut(s) 555, 1869, 1881
MspA1I CMGCKG 1 cut(s) 1024
MspI CCGG 4 cut(s) 120, 251, 317, 793
MspR9I CCNGG 5 cut(s) 251, 252, 847, 1425, 1815
Mva1269I GAATGC 2 cut(s) 1162, 1783
MvaI CCWGG 3 cut(s) 847, 1425, 1815
MvnI CGCG 3 cut(s) 66, 768, 1173
MwoI GCNNNNNNNGC 7 cut(s) 116, 223, 774, 819, 1015, 1246, 1517
NaeI GCCGGC 1 cut(s) 121
NarI GGCGCC 1 cut(s) 108
NciI CCSGG 2 cut(s) 251, 252
NdeII GATC 4 cut(s) 534, 620, 1060, 1212
NgoMIV GCCGGC 1 cut(s) 119
NlaIII CATG 3 cut(s) 508, 1483, 1748
NmeAIII GCCGAG 3 cut(s) 84, 207, 246
NmuCI GTSAC 2 cut(s) 865, 1690
NspI RCATGY 2 cut(s) 508, 1483
PaeR7I CTCGAG 1 cut(s) 443
PcsI WCGNNNNNNNCGW 5 cut(s) 183, 234, 273, 332, 1309
PctI GAATGC 2 cut(s) 1162, 1783
PdiI GCCGGC 1 cut(s) 121
PfeI GAWTC 4 cut(s) 99, 942, 1106, 1561
PkrI GCNGC 7 cut(s) 333, 694, 767, 1519, 1618, 1735, 1782
PleI GAGTC 1 cut(s) 447
PluTI GGCGCC 1 cut(s) 111
PpsI GAGTC 1 cut(s) 447
PpuMI RGGWCCY 1 cut(s) 247
PshAI GACNNNNGTC 1 cut(s) 1119
Psp1406I AACGTT 1 cut(s) 914
Psp5II RGGWCCY 1 cut(s) 247
Psp6I CCWGG 3 cut(s) 845, 1423, 1813
PspFI CCCAGC 2 cut(s) 1024, 1580
PspGI CCWGG 3 cut(s) 845, 1423, 1813
PspPI GGNCC 6 cut(s) 117, 247, 674, 763, 1571, 1834
PspPPI RGGWCCY 1 cut(s) 247
PvuII CAGCTG 1 cut(s) 1024
RsaI GTAC 6 cut(s) 69, 134, 200, 306, 844, 1450
RsaNI GTAC 6 cut(s) 68, 133, 199, 305, 843, 1449
SalI GTCGAC 2 cut(s) 239, 425
SaqAI TTAA 3 cut(s) 555, 1869, 1881
SatI GCNGC 7 cut(s) 332, 693, 766, 1518, 1617, 1734, 1781
Sau3AI GATC 4 cut(s) 534, 620, 1060, 1212
Sau96I GGNCC 6 cut(s) 117, 247, 674, 763, 1571, 1834
ScaI AGTACT 1 cut(s) 306
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 5 cut(s) 251, 252, 847, 1425, 1815
SduI GDGCHC 1 cut(s) 934
SexAI ACCWGGT 1 cut(s) 1813
SfaNI GCATC 6 cut(s) 216, 318, 361, 640, 1129, 1258
SfoI GGCGCC 1 cut(s) 109
Sfr274I CTCGAG 1 cut(s) 443
SinI GGWCC 3 cut(s) 247, 674, 1571
SlaI CTCGAG 1 cut(s) 443
SmaI CCCGGG 1 cut(s) 252
SmlI CTYRAG 2 cut(s) 443, 1254
SmoI CTYRAG 2 cut(s) 443, 1254
SpeI ACTAGT 1 cut(s) 1445
Sse9I AATT 8 cut(s) 311, 398, 654, 710, 814, 1000, 1601, 1636
SsiI CCGC 8 cut(s) 64, 127, 659, 693, 766, 790, 902, 1629
SspDI GGCGCC 1 cut(s) 107
SspI AATATT 1 cut(s) 1873
SspMI CTAG 7 cut(s) 494, 516, 734, 1050, 1151, 1446, 1620
StyD4I CCNGG 5 cut(s) 249, 250, 845, 1423, 1813
StyI CCWWGG 1 cut(s) 1827
TaaI ACNGT 5 cut(s) 352, 887, 1278, 1534, 1660
TaiI ACGT 6 cut(s) 189, 731, 917, 1315, 1498, 1778
TaqI TCGA 8 cut(s) 240, 288, 309, 326, 426, 444, 1502, 1717
TasI AATT 8 cut(s) 311, 398, 654, 710, 814, 1000, 1601, 1636
TatI WGTACW 2 cut(s) 304, 1448
TauI GCSGC 2 cut(s) 695, 768
TfiI GAWTC 4 cut(s) 99, 942, 1106, 1561
Tru1I TTAA 3 cut(s) 555, 1869, 1881
Tru9I TTAA 3 cut(s) 555, 1869, 1881
TscAI CASTG 5 cut(s) 892, 898, 1332, 1656, 1695
TseFI GTSAC 2 cut(s) 865, 1690
TseI GCWGC 5 cut(s) 331, 1517, 1616, 1733, 1780
Tsp45I GTSAC 2 cut(s) 865, 1690
TspDTI ATGAA 3 cut(s) 454, 911, 1751
TspGWI ACGGA 5 cut(s) 226, 353, 969, 1679, 1860
TspMI CCCGGG 1 cut(s) 250
TspRI CASTG 5 cut(s) 892, 898, 1332, 1656, 1695
VpaK11BI GGWCC 3 cut(s) 247, 674, 1571
XapI RAATTY 5 cut(s) 311, 398, 710, 1601, 1636
XceI RCATGY 2 cut(s) 508, 1483
XhoI CTCGAG 1 cut(s) 443
XmaI CCCGGG 1 cut(s) 250
XmiI GTMKAC 4 cut(s) 240, 405, 426, 1056
XspI CTAG 7 cut(s) 494, 516, 734, 1050, 1151, 1446, 1620
ZraI GACGTC 1 cut(s) 187
ZrmI AGTACT 1 cut(s) 306
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.