FvH4_1g05650

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012242
Physical Location & Seq
Reverse (-)
2985610 .. 2990831
5222 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05650.t1

Sequence Viewer

Length: 3072 bp
ATGACGCCTGTAGTTCCATACTCTATAAGCAATGTCTCAGTTTTTCCTGGAACTGCCTTTACCTCAAGAAAGACCAATTCTTTAACCAAATTCAATTTTTCTAGGAATTCCGCTAGACATACTTTATCCCCCCAGAGTTTTCTTTTACCTTTTTCGACCTCTATTAGATTGTTTCCGTTGTACAACAACAGATGCCCTGTACATCATAGTTCTAGAACCTATGTAATATCTGCCACAGGGACTGACGTAGCAGTGGAGCAACCAGACTCAGCCACTGCAGAAGCTACTACTGAAGCCTTGGATAATTCCTCCGATGCAGCTGAAACTATTGAAAAATCCTCCAGCTCTGATGCAAGTTCCGGTCCATCTCAAGCAAGGCGTGCAAGACCTGGTAGGCAAAGCGAGATGCCGCCTGTTAAGAATGAGGAGCTGGTCCCTGGTGCAACTTTTACAGGGAAAGTGAGATCAATCCAGCCATTTGGTGCTTTCATTGATTTTGGAGCTTTCACAGATGGCCTGGTACATGTTTCACAGTTGAGTGATACTTATGTAAAAGATGTAGGAAGTGTTGTTTCTGTTGGGCAAGAGGTGAAGGTGACATTAGTTGAAGCCAATATGGAGACTAAGCGGATTTCTCTCACTATGCGTGAAGGGAAAGATGCTTCTTCCAGTAGTGATAGGGGAGGATCTGATAGGAGAGGTGGTCCAAAGAAAGGAGAGAGGAAAAATGAGGGCAGAAAAAGTTCAAAGTTTGCCAAGGGGCAGGACCTAGTAGGCACAGTGAAGAATTTGGTCAGGGCTGGTGCTTTTATCTCTCTTCCTGAGGGGGAGGAAGGTTTCTTGCCTCAGTCTGAGGAAGTTGATGATGGATTTGCAAGCATGATGGGTGAGACCTCGCTGGAGGTTGGCCAGGAAATTAATGTCCGTGTGTTGCGTATTAGTAGAGGACAAGTGACCTTGACAATGAAGAAAGAAGAAGATCTTCTGAAGTCAGAGTCCCAGATCACTCAAGGAGTCATCCACACAGCCACAAACCCATTTCTCCTGGCTTTTCGCCAAAACAAGGATGTTGCTGCATTTTTGGACGAAAGAGAGAAAACAACTAAAGAAACTGTAACTCCAAAGAGTACGAAAGAATCAACACAGGAAGTGCTGGATAAACAAGTAAACAGTGACATGCAAACTCTTGACGTCCCTTCTGCCGTGGATGAAAGTATTGAGAACGATGGTGCTCCTTTAGAGGTGGCTGATGTGGGAGCCAGTGAAGTAGATGATGCATCAAGCAAAGAGGACCAGGAAAACACTGTTTCTAGTTCAACAGAAACTATAGAAACTACAGATGGTGCAGTCCAAGACATACAAAAGGAAGAGGTGAGTTCCAAAATGTTGGATCCTGAAGAAAGTATATCTCCTACCACAGATTCTGCTATTCAGGAGTCTCCAACTGATGGAGTGGAAAATGATGCAAATCCAGATTTGTCTAGTGAAATAGCTAAGCAAGCATTGCCATCAGATATTGCGATAGCTGAAGAGGTTATAGAGAGTAAAGTAGATGACACAATAGCAAAAGTTGAACCGCAAATAGAACCACCTACTTCAGAGAGCGAAAGTCCTTCAACTCAACTTACTGTAGACGAGGAAGTGCAACCAGCTCCTAATACAAGTGGAAGTATCACCAGCTCAGATGTTCAACCAGATCTTGCTTCTCCTCAAGAGACAAAAGCAACTATATCACCAGCTCTTGTAAAACAGCTCCGTGACGAGTCAGGAGCTGGAATGATGGACTGCAAAAAGGCTTTGTCAGAGTCTGGAGGAGACATTGTCAAAGCCCAGGAGTTCCTTAGAAAGAAAGGCTTAGCAAGTGCAGACAAGAAAGCCAGCAGAGTCACCGCTGAAGGGAGAATAGGTTCGTACATTCATGATAGCAGGATTGGTATCTTACTGGAGGTAAACTGCGAGACAGATTTTGTTTCCCGGGGTGACATTTTCAAGGAGCTGGTCGATGATTTAGCCATGCAAGCAGCTGCATGCCCTCAAGTACAGTACGTTACTACAGAAGATGTTCCGGAGGAGTTTGTAAACAAGGAAAGAGAGATTGAGATGCAGAAAGAAGATCTTTTGTCAAAGCCAGAGCAGATTAGATCAAAGATTGTTGACGGGAGGATCAAGAAGAGGCTTGATGAGCTGGCATTGCTTGAGCAGCCCTACATCAAGAATGATAAGGTGGTGGTGAAGGACTGGGTGAAGCAAACGATTGCAACCATTGGGGAAAACATAAAAGTGAAGAGGTTTGTGCGTTTCAATCTGGGCGAAGGCTTGGAAAAACGGAGTCAGGATTTTGCAGCTGAAGTGGCTGCCCAAACTGCAGCAAAAAAGGTGCCTGCAGCAGGGAAAGAACAGCCTGCTGCAGTTGAAGCAAAGGAAATAGTTCAGAAGGCACCAACTGTAGCTATTTCTGCTGCTTTGGTTAAACAACTAAGGGAAGAAACTGGAGCGGGAATGATGGACTGCAAGAAAGCTCTCTCTGAAACTGGAGGGGATATAGAGAAGGCACAAGAGTACCTCAGAAAGAAGGGTCTTTCTTCTGCCGAAAAGAAATCCAGTCGGCTTGCTGCTGAAGGCAGGATAGGATCTTACATTCATGATGCTCGCATTGGAGTTCTGATTGAAGTCAACTCTGAGACAGACTTTGTTGGTCGAAGTGAAAAGTTCAAGGAGTTGGTTGATGACCTGGCAATGCAAGTTGTGGCCTGCCCACAGGTGCAATTTGTATCCATCGAAGACATTCCAGAGAGTATTGTAAAGAAGGAAAAAGAGCTTGAGATGCAGAGGGAGGATCTTCTGTCCAAACCTGAAAACATTAGGGAGAGAATCGTGGAGGGAAGGATCTCAAAGAGGTTTGGGGAGCTTGCTCTGTTGGAGCAACCTTTTATCAAAGATGATAGTTTATTGGTGAAGGATCTGGTGAAGCAGACTGTTGCTGCTCTTGGGGAAAATATAAAAGTTCGAAGGTTTGTTCGGTTCACTCTTGGGGAGACAGTTGAGGGTACGAAATCAGAAGCTGAAGCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF_TS S1

Protein Analysis

1024

Amino Acids

111.36

Weight (kDa)

4.89

Isoelectric Point (pI)

48.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1_RRP5 PF23459 145 - 210 3.2e-06 RRP5 S1 domain
S1 PF00575 146 - 216 1.8e-15 S1 RNA binding domain
S1 PF00575 250 - 315 9e-07 S1 RNA binding domain
EF_TS PF00889 626 - 771 1e-28 Elongation factor TS
EF_TS PF00889 868 - 1012 1.5e-28 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29060 AT4G29060
fragaria_vesca FvH4_1g05650
malus_domestica MD02G1057500.v1.1 MD15G1191900.v1.1
prunus_persica Prupe.7G225300_v2.0.a1
pyrus_communis pycom02g04660
rosa_chinensis RchiOBHm_Chr2g0091281
rosa_laevigata RLG00000016202
rosa_multiflora Rmu_sc0002270.1_g000032
rosa_roxburghii Rroxscaffold_2G00150270
rosa_rugosa Rorug02G0015600 Rorug02G0015700 Rorug02G0015800
rosa_samantha Rh2AG061000 Rh2BG060000 Rh2CG062000 Rh2DG060500
rosa_wichuraiana Rw2G004880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1194
AccB1I GGYRCC 2 cut(s) 2377, 2437
AccB7I CCANNNNNTGG 1 cut(s) 1448
AccBSI CCGCTC 1 cut(s) 2495
AccI GTMKAC 1 cut(s) 1632
AccIII TCCGGA 1 cut(s) 2065
AciI CCGC 6 cut(s) 111, 410, 628, 1577, 1890, 2495
AclWI GGATC 8 cut(s) 694, 1385, 1398, 2171, 2638, 2844, 2894, 2967
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 3 cut(s) 89, 106, 787
AcuI CTGAAG 8 cut(s) 312, 1007, 1416, 1548, 1581, 1914, 2367, 2637
AcyI GRCGYC 2 cut(s) 5, 1191
AfaI GTAC 9 cut(s) 182, 201, 522, 1129, 1913, 2040, 2045, 2561, 3049
AfiI CCNNNNNNNGG 5 cut(s) 713, 1063, 1448, 1896, 2387
AflIII ACRYGT 1 cut(s) 523
AjnI CCWGG 9 cut(s) 46, 388, 436, 516, 909, 1044, 1293, 1830, 2730
AloI GAACNNNNNNTCC 2 cut(s) 1891, 1923
Alw21I GWGCWC 1 cut(s) 1234
Alw26I GTCTC 9 cut(s) 40, 614, 884, 1443, 1709, 1809, 1952, 2675, 3029
AlwI GGATC 8 cut(s) 694, 1385, 1398, 2171, 2638, 2844, 2894, 2967
AlwNI CAGNNNCTG 6 cut(s) 242, 275, 1424, 1772, 1808, 3062
Ama87I CYCGRG 1 cut(s) 1974
Aor13HI TCCGGA 1 cut(s) 2065
AoxI GGCC 3 cut(s) 514, 907, 2748
ApoI RAATTY 3 cut(s) 89, 106, 787
AseI ATTAAT 1 cut(s) 918
Asp700I GAANNNNTTC 6 cut(s) 742, 981, 1906, 2061, 2427, 2784
AspS9I GGNCC 5 cut(s) 362, 433, 704, 766, 1291
AsuC2I CCSGG 2 cut(s) 1975, 1976
AsuII TTCGAA 1 cut(s) 3007
AvaI CYCGRG 1 cut(s) 1974
AvaII GGWCC 5 cut(s) 362, 433, 704, 766, 1291
AxyI CCTNAGG 1 cut(s) 822
BaeI ACNNNNGTAYC 4 cut(s) 534, 567, 2543, 2576
BalI TGGCCA 1 cut(s) 909
BamHI GGATCC 1 cut(s) 1390
BanI GGYRCC 2 cut(s) 2377, 2437
BbsI GAAGAC 1 cut(s) 2787
Bbv12I GWGCWC 1 cut(s) 1234
BceAI ACGGC 1 cut(s) 1187
BciT130I CCWGG 9 cut(s) 48, 390, 438, 518, 911, 1046, 1295, 1832, 2732
BciVI GTATCC 1 cut(s) 2782
BcnI CCSGG 2 cut(s) 1975, 1976
BcoDI GTCTC 9 cut(s) 40, 614, 884, 1443, 1709, 1809, 1952, 2675, 3029
BfaI CTAG 6 cut(s) 102, 114, 213, 770, 1311, 1482
BfuI GTATCC 1 cut(s) 2782
BglII AGATCT 3 cut(s) 979, 1696, 2113
BlpI GCTNAGC 2 cut(s) 1494, 1855
Bme18I GGWCC 5 cut(s) 362, 433, 704, 766, 1291
BmeT110I CYCGRG 1 cut(s) 1974
BmgT120I GGNCC 5 cut(s) 362, 433, 704, 766, 1291
BmiI GGNNCC 5 cut(s) 435, 1258, 1392, 2379, 2439
BmrI ACTGGG 1 cut(s) 2248
BmuI ACTGGG 1 cut(s) 2248
BpiI GAAGAC 1 cut(s) 2787
BpmI CTGGAG 6 cut(s) 325, 920, 1830, 1964, 2511, 2553
Bpu1102I GCTNAGC 2 cut(s) 1494, 1855
Bpu14I TTCGAA 1 cut(s) 3007
BpuEI CTTGAG 7 cut(s) 49, 354, 993, 1695, 2019, 2216, 2840
BpuMI CCSGG 2 cut(s) 1975, 1976
BsaBI GATNNNNATC 2 cut(s) 1467, 1934
BsaHI GRCGYC 2 cut(s) 5, 1191
BsaI GGTCTC 1 cut(s) 884
BsaJI CCNNGG 7 cut(s) 297, 436, 756, 1203, 1830, 1974, 1975
BsaWI WCCGGW 2 cut(s) 359, 2065
BsaXI ACNNNNNCTCC 8 cut(s) 688, 718, 1102, 1132, 1806, 1836, 1891, 1921
Bsc4I CCNNNNNNNGG 5 cut(s) 713, 1063, 1448, 1896, 2387
Bse1I ACTGG 7 cut(s) 669, 1260, 1947, 2243, 2494, 2536, 2601
Bse21I CCTNAGG 1 cut(s) 822
Bse3DI GCAATG 4 cut(s) 37, 1502, 2189, 2742
Bse8I GATNNNNATC 2 cut(s) 1467, 1934
BseAI TCCGGA 1 cut(s) 2065
BseBI CCWGG 9 cut(s) 48, 390, 438, 518, 911, 1046, 1295, 1832, 2732
BseDI CCNNGG 7 cut(s) 297, 436, 756, 1203, 1830, 1974, 1975
BseGI GGATG 3 cut(s) 1017, 1072, 1213
BseJI GATNNNNATC 2 cut(s) 1467, 1934
BseLI CCNNNNNNNGG 5 cut(s) 713, 1063, 1448, 1896, 2387
BseMI GCAATG 4 cut(s) 37, 1502, 2189, 2742
BseMII CTCAG 8 cut(s) 51, 282, 813, 843, 860, 1695, 2578, 2670
BseNI ACTGG 7 cut(s) 669, 1260, 1947, 2243, 2494, 2536, 2601
BseRI GAGGAG 4 cut(s) 440, 1698, 1827, 2084
BsgI GTGCAG 2 cut(s) 1365, 1884
BshFI GGCC 3 cut(s) 516, 909, 2750
BshNI GGYRCC 2 cut(s) 2377, 2437
BsiHKAI GWGCWC 1 cut(s) 1234
BsiHKCI CYCGRG 1 cut(s) 1974
BsiSI CCGG 3 cut(s) 360, 1975, 2066
BslFI GGGAC 4 cut(s) 253, 419, 982, 1178
BslI CCNNNNNNNGG 5 cut(s) 713, 1063, 1448, 1896, 2387
BsmAI GTCTC 9 cut(s) 40, 614, 884, 1443, 1709, 1809, 1952, 2675, 3029
BsmFI GGGAC 4 cut(s) 253, 419, 982, 1178
BsnI GGCC 3 cut(s) 516, 909, 2750
Bso31I GGTCTC 1 cut(s) 884
BsoBI CYCGRG 1 cut(s) 1974
Bsp119I TTCGAA 1 cut(s) 3007
Bsp1286I GDGCHC 1 cut(s) 1234
Bsp13I TCCGGA 1 cut(s) 2065
Bsp1407I TGTACA 2 cut(s) 180, 199
Bsp1720I GCTNAGC 2 cut(s) 1494, 1855
BspACI CCGC 6 cut(s) 111, 410, 628, 1577, 1890, 2495
BspANI GGCC 3 cut(s) 516, 909, 2750
BspCNI CTCAG 8 cut(s) 50, 281, 814, 844, 859, 1694, 2577, 2671
BspEI TCCGGA 1 cut(s) 2065
BspHI TCATGA 2 cut(s) 1918, 2641
BspLI GGNNCC 5 cut(s) 435, 1258, 1392, 2379, 2439
BspMAI CTGCAG 4 cut(s) 280, 2368, 2386, 2410
BspPI GGATC 8 cut(s) 694, 1385, 1398, 2171, 2638, 2844, 2894, 2967
BspT104I TTCGAA 1 cut(s) 3007
BspT107I GGYRCC 2 cut(s) 2377, 2437
BspTNI GGTCTC 1 cut(s) 884
BsrBI CCGCTC 1 cut(s) 2495
BsrDI GCAATG 4 cut(s) 37, 1502, 2189, 2742
BsrGI TGTACA 2 cut(s) 180, 199
BsrI ACTGG 7 cut(s) 669, 1260, 1947, 2243, 2494, 2536, 2601
BssECI CCNNGG 7 cut(s) 297, 436, 756, 1203, 1830, 1974, 1975
BssNI GRCGYC 2 cut(s) 5, 1191
BssT1I CCWWGG 2 cut(s) 297, 756
Bst2UI CCWGG 9 cut(s) 48, 390, 438, 518, 911, 1046, 1295, 1832, 2732
Bst6I CTCTTC 5 cut(s) 822, 1362, 1524, 2165, 2279
BstACI GRCGYC 2 cut(s) 5, 1191
BstAPI GCANNNNNTGC 2 cut(s) 380, 1504
BstAUI TGTACA 2 cut(s) 180, 199
BstBI TTCGAA 1 cut(s) 3007
BstDSI CCRYGG 1 cut(s) 1203
BstENI CCTNNNNNAGG 1 cut(s) 2385
BstF5I GGATG 3 cut(s) 1017, 1072, 1213
BstMAI GTCTC 9 cut(s) 40, 614, 884, 1443, 1709, 1809, 1952, 2675, 3029
BstNI CCWGG 9 cut(s) 48, 390, 438, 518, 911, 1046, 1295, 1832, 2732
BstNSI RCATGY 3 cut(s) 527, 1180, 2031
BstV2I GAAGAC 1 cut(s) 2787
BstX2I RGATCY 9 cut(s) 686, 979, 1390, 1696, 2113, 2630, 2836, 2886, 2959
BstXI CCANNNNNNTGG 2 cut(s) 479, 1387
BstYI RGATCY 9 cut(s) 686, 979, 1390, 1696, 2113, 2630, 2836, 2886, 2959
Bsu36I CCTNAGG 1 cut(s) 822
BsuI GTATCC 1 cut(s) 2782
BsuRI GGCC 3 cut(s) 516, 909, 2750
BtgI CCRYGG 1 cut(s) 1203
BtsCI GGATG 3 cut(s) 1017, 1072, 1213
BtsI GCAGTG 2 cut(s) 258, 273
BtsIMutI CAGTG 6 cut(s) 258, 273, 786, 1177, 1267, 1302
CaiI CAGNNNCTG 6 cut(s) 242, 275, 1424, 1772, 1808, 3062
CciI TCATGA 2 cut(s) 1918, 2641
Cfr13I GGNCC 5 cut(s) 362, 433, 704, 766, 1291
Cfr9I CCCGGG 1 cut(s) 1974
CseI GACGC 1 cut(s) 13
CsiI ACCWGGT 1 cut(s) 388
Csp6I GTAC 9 cut(s) 181, 200, 521, 1128, 1912, 2039, 2044, 2560, 3048
CviAII CATG 7 cut(s) 524, 880, 1177, 1919, 2014, 2028, 2642
CviQI GTAC 9 cut(s) 181, 200, 521, 1128, 1912, 2039, 2044, 2560, 3048
EaeI YGGCCR 1 cut(s) 907
Eam1104I CTCTTC 5 cut(s) 822, 1362, 1524, 2165, 2279
EarI CTCTTC 5 cut(s) 822, 1362, 1524, 2165, 2279
Eco130I CCWWGG 2 cut(s) 297, 756
Eco31I GGTCTC 1 cut(s) 884
Eco47I GGWCC 5 cut(s) 362, 433, 704, 766, 1291
Eco57I CTGAAG 8 cut(s) 312, 1007, 1416, 1548, 1581, 1914, 2367, 2637
Eco81I CCTNAGG 1 cut(s) 822
Eco88I CYCGRG 1 cut(s) 1974
EcoNI CCTNNNNNAGG 1 cut(s) 2385
EcoO109I RGGNCCY 1 cut(s) 766
EcoRI GAATTC 1 cut(s) 106
EcoRII CCWGG 9 cut(s) 46, 388, 436, 516, 909, 1044, 1293, 1830, 2730
EcoT14I CCWWGG 2 cut(s) 297, 756
EcoT22I ATGCAT 1 cut(s) 1279
ErhI CCWWGG 2 cut(s) 297, 756
FaeI CATG 7 cut(s) 527, 883, 1180, 1922, 2017, 2031, 2645
FalI AAGNNNNNCTT 6 cut(s) 966, 998, 1838, 1870, 2100, 2132
FaqI GGGAC 4 cut(s) 253, 419, 982, 1178
FatI CATG 7 cut(s) 523, 879, 1176, 1918, 2013, 2027, 2641
FauI CCCGC 1 cut(s) 2488
FblI GTMKAC 1 cut(s) 1632
FokI GGATG 3 cut(s) 1004, 1079, 1220
FspBI CTAG 6 cut(s) 102, 114, 213, 770, 1311, 1482
GsuI CTGGAG 6 cut(s) 325, 920, 1830, 1964, 2511, 2553
HaeIII GGCC 3 cut(s) 516, 909, 2750
HapII CCGG 3 cut(s) 360, 1975, 2066
HgaI GACGC 1 cut(s) 13
Hin1I GRCGYC 2 cut(s) 5, 1191
Hin1II CATG 7 cut(s) 527, 883, 1180, 1922, 2017, 2031, 2645
HincII GTYRAC 2 cut(s) 2155, 2674
HindII GTYRAC 2 cut(s) 2155, 2674
HpaII CCGG 3 cut(s) 360, 1975, 2066
Hpy166II GTNNAC 7 cut(s) 1168, 1633, 1951, 2080, 2155, 2674, 3024
Hpy8I GTNNAC 7 cut(s) 1168, 1633, 1951, 2080, 2155, 2674, 3024
HpyCH4IV ACGT 3 cut(s) 246, 1191, 2046
HpySE526I ACGT 3 cut(s) 246, 1191, 2046
Hsp92I GRCGYC 2 cut(s) 5, 1191
Hsp92II CATG 7 cut(s) 527, 883, 1180, 1922, 2017, 2031, 2645
Kpn2I TCCGGA 1 cut(s) 2065
MabI ACCWGGT 1 cut(s) 388
MaeI CTAG 6 cut(s) 102, 114, 213, 770, 1311, 1482
MaeII ACGT 3 cut(s) 246, 1191, 2046
MaeIII GTNAC 8 cut(s) 595, 952, 1114, 1172, 1757, 1885, 1979, 2047
MbiI CCGCTC 1 cut(s) 2495
MflI RGATCY 9 cut(s) 686, 979, 1390, 1696, 2113, 2630, 2836, 2886, 2959
MhlI GDGCHC 1 cut(s) 1234
MlsI TGGCCA 1 cut(s) 909
MluCI AATT 8 cut(s) 76, 89, 94, 106, 304, 787, 915, 2765
MluNI TGGCCA 1 cut(s) 909
MlyI GAGTC 8 cut(s) 260, 1004, 1023, 1445, 1772, 1814, 1893, 2338
MmeI TCCRAC 3 cut(s) 1368, 1466, 2898
Mox20I TGGCCA 1 cut(s) 909
Mph1103I ATGCAT 1 cut(s) 1279
MroI TCCGGA 1 cut(s) 2065
MroXI GAANNNNTTC 6 cut(s) 742, 981, 1906, 2061, 2427, 2784
MscI TGGCCA 1 cut(s) 909
MseI TTAA 4 cut(s) 83, 417, 918, 2469
MslI CAYNNNNRTG 1 cut(s) 2279
Msp20I TGGCCA 1 cut(s) 909
MspA1I CMGCKG 4 cut(s) 320, 1892, 2024, 2345
MspI CCGG 3 cut(s) 360, 1975, 2066
MvaI CCWGG 9 cut(s) 48, 390, 438, 518, 911, 1046, 1295, 1832, 2732
NciI CCSGG 2 cut(s) 1975, 1976
NlaIII CATG 7 cut(s) 527, 883, 1180, 1922, 2017, 2031, 2645
NlaIV GGNNCC 5 cut(s) 435, 1258, 1392, 2379, 2439
NmuCI GTSAC 6 cut(s) 595, 952, 1172, 1757, 1885, 1979
NsiI ATGCAT 1 cut(s) 1279
NspI RCATGY 3 cut(s) 527, 1180, 2031
NspV TTCGAA 1 cut(s) 3007
PaeI GCATGC 1 cut(s) 2031
PagI TCATGA 2 cut(s) 1918, 2641
PciI ACATGT 1 cut(s) 523
PdmI GAANNNNTTC 6 cut(s) 742, 981, 1906, 2061, 2427, 2784
PfeI GAWTC 3 cut(s) 1136, 1421, 2871
PflFI GACNNNGTC 1 cut(s) 1820
PflMI CCANNNNNTGG 1 cut(s) 1448
PfoI TCCNGGA 1 cut(s) 46
PleI GAGTC 8 cut(s) 260, 1003, 1022, 1444, 1771, 1813, 1892, 2337
PpsI GAGTC 8 cut(s) 260, 1003, 1022, 1444, 1771, 1813, 1892, 2337
PpuMI RGGWCCY 1 cut(s) 766
PscI ACATGT 1 cut(s) 523
PshBI ATTAAT 1 cut(s) 918
Psp5II RGGWCCY 1 cut(s) 766
Psp6I CCWGG 9 cut(s) 46, 388, 436, 516, 909, 1044, 1293, 1830, 2730
PspGI CCWGG 9 cut(s) 46, 388, 436, 516, 909, 1044, 1293, 1830, 2730
PspN4I GGNNCC 5 cut(s) 435, 1258, 1392, 2379, 2439
PspPI GGNCC 5 cut(s) 362, 433, 704, 766, 1291
PspPPI RGGWCCY 1 cut(s) 766
PsrI GAACNNNNNNTAC 2 cut(s) 43, 75
PstI CTGCAG 4 cut(s) 280, 2368, 2386, 2410
PstNI CAGNNNCTG 6 cut(s) 242, 275, 1424, 1772, 1808, 3062
PsuI RGATCY 9 cut(s) 686, 979, 1390, 1696, 2113, 2630, 2836, 2886, 2959
PsyI GACNNNGTC 1 cut(s) 1820
PvuII CAGCTG 3 cut(s) 320, 2024, 2345
RsaI GTAC 9 cut(s) 182, 201, 522, 1129, 1913, 2040, 2045, 2561, 3049
RsaNI GTAC 9 cut(s) 181, 200, 521, 1128, 1912, 2039, 2044, 2560, 3048
RseI CAYNNNNRTG 1 cut(s) 2279
SaqAI TTAA 4 cut(s) 83, 417, 918, 2469
Sau96I GGNCC 5 cut(s) 362, 433, 704, 766, 1291
SchI GAGTC 8 cut(s) 260, 1004, 1023, 1445, 1772, 1814, 1893, 2338
SduI GDGCHC 1 cut(s) 1234
SexAI ACCWGGT 1 cut(s) 388
SfuI TTCGAA 1 cut(s) 3007
SinI GGWCC 5 cut(s) 362, 433, 704, 766, 1291
SmaI CCCGGG 1 cut(s) 1976
SmiMI CAYNNNNRTG 1 cut(s) 2279
SmlI CTYRAG 7 cut(s) 64, 369, 1008, 1710, 2034, 2195, 2819
SmoI CTYRAG 7 cut(s) 64, 369, 1008, 1710, 2034, 2195, 2819
SphI GCATGC 1 cut(s) 2031
Sse9I AATT 8 cut(s) 76, 89, 94, 106, 304, 787, 915, 2765
SsiI CCGC 6 cut(s) 111, 410, 628, 1577, 1890, 2495
SspMI CTAG 6 cut(s) 102, 114, 213, 770, 1311, 1482
StyI CCWWGG 2 cut(s) 297, 756
TaiI ACGT 3 cut(s) 249, 1194, 2049
TaqI TCGA 5 cut(s) 155, 2001, 2698, 2778, 3007
TasI AATT 8 cut(s) 76, 89, 94, 106, 304, 787, 915, 2765
TatI WGTACW 3 cut(s) 180, 199, 2038
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 3 cut(s) 1136, 1421, 2871
Tru1I TTAA 4 cut(s) 83, 417, 918, 2469
Tru9I TTAA 4 cut(s) 83, 417, 918, 2469
TscAI CASTG 6 cut(s) 258, 280, 786, 1177, 1267, 1309
TseFI GTSAC 6 cut(s) 595, 952, 1172, 1757, 1885, 1979
Tsp45I GTSAC 6 cut(s) 595, 952, 1172, 1757, 1885, 1979
TspDTI ATGAA 5 cut(s) 478, 980, 1224, 1907, 2630
TspGWI ACGGA 4 cut(s) 165, 914, 1745, 2341
TspMI CCCGGG 1 cut(s) 1974
TspRI CASTG 6 cut(s) 258, 280, 786, 1177, 1267, 1309
Tth111I GACNNNGTC 1 cut(s) 1820
Van91I CCANNNNNTGG 1 cut(s) 1448
VpaK11BI GGWCC 5 cut(s) 362, 433, 704, 766, 1291
VspI ATTAAT 1 cut(s) 918
XagI CCTNNNNNAGG 1 cut(s) 2385
XapI RAATTY 3 cut(s) 89, 106, 787
XbaI TCTAGA 1 cut(s) 212
XceI RCATGY 3 cut(s) 527, 1180, 2031
XmaI CCCGGG 1 cut(s) 1974
XmiI GTMKAC 1 cut(s) 1632
XmnI GAANNNNTTC 6 cut(s) 742, 981, 1906, 2061, 2427, 2784
XspI CTAG 6 cut(s) 102, 114, 213, 770, 1311, 1482
ZraI GACGTC 1 cut(s) 1192
Zsp2I ATGCAT 1 cut(s) 1279
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.