RLG00000016202

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012242
Physical Location & Seq
Reverse (-)
4712203 .. 4718661
6459 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016202

Sequence Viewer

Length: 3183 bp
ATGAGATTGCTGTCGGACGAGTTGTGGGTCTTAAGGCTGGGCTCTTGGGTTAGCTCTGCTCGAAGATTGGAAGTGGTGTTCTGGCTTGGTTATGTTTTCGATGCTCTTAGGCGGGTGGAGCTCCTCATTCTGATGGCGGTGATGGTGGTCGAAGGTGGTGCTTTTGATGGATTTGTACGAAGGGTGAAGCAGAGCTTGATTGGGCTTCACCGGGCCTCGAAACACATTTTGAAAGTTTTGTTGGCTCAATTTTTGGTTAAAGGTGAGCGAGGGATGAAATCTAGAACCTATGTAGTATCTGCCACAGGGACTGACGTAGCAGTGGAGCAACCAGACTCACCCGTTGCAGAAGCTTCAACTGAAGCTTTGGATAAATCCTCTGATGCAGCTGAAACTAGTGAAAAATCCTCCAACTCTGACGCAAGTTCCAGTCCATCTCAGGCAAGGCGTGCAAGAACTAGTAGGCAAAGTGAGATGTCACCTGTTAAGAATGAGGAGCTGGTTCCTGGCGCAACTTTTACAGGGAAAGTGAGATCAATCCAGCCATTTGGTGCTTTCATTGATTTTGGAGCTTTCACAGATGGCCTGGTACATGTTTCACAGCTGAGTGATACTTATGTAAAAGATGTAGGAAGTGTTGTTTCTGTTGGGCAAGAGGTGAAGGTGACATTAGTTGAAGCCAATATGGAGACTAAGCGGATATCTCTCACTATGCGTGAAGGCAAAGATGCTTCCGGTAGTGATAGGGGTGGATCTGATAGGAGAGGTGGTCCAAAGAAAGGAGAGAGGAAAAACGAGGGAAGAAAAAGTTCAAAGTTTGCTAAGGGGCAGGACTTAGTGGGCACGGTGAAGAATTTGGTGAGGGCTGGTGCTTTTATATCTCTTCCTGAGGGGGAAGAAGGTTTCTTGCCTCAGTCCGAGGAAGCTGATGAAGGATTTGCAAGCATTATGGGGGAGACCTCTATGGAGGTTGGCCAGGAAGTTAATGTCCGTGTGTTGCGTATTAGTAGAGGACAAGTAACCTTGACAATGAAGAAAGAAGAAGATATTCAGAAGTCAGAGTCCCAGATCACTCAAGGAGTCATCCACACAGCAACAAACCCATTTCTCCTGGCTTTTCGCCAAAACAAGGATATTGCTGCATTTTTGGACGAAAGAGAGAAAGTAGAGAAAGCAACTAAAGAAACTGTAAATCCAAAGAGTACAGAAGAATCAACACAGAAGTTTAATGAAACTGAGAATGAGTCCAATATTCATGAAGTGCTGGACAAACAAGTAAGTAGTGATGAGCAAAGTCTTGGTGTCTCTTCTACCGTGGACGAAAGTATTGAAAATGATGGTGCTCCTTTAGAGGTGGTTGATGTTGGAGCCAGTGGAATAGATGATACGTCAAGCAAAGAAGACCAGGAAACCATCGTTTCTAGTTCAACAGAAACTCTAGAAACTATGGATGGTGCAGTCCAAGACATACAAAAAGAAGAGATGAGTTCTGAAATTTTGGTTCCTGAAGAAAGTATATCTACCACAGATTCTACAATTCAGGAGTCTCCGTCAACTGATGGTGTGGAAAATGATTCAAATCCAGATTCGTCTAGTGAAATAGCTAATCAAGCATTGCCATCAGATATTACAGCAACTGAAGAGGTTATAGAGAGTAAAGTAGACGACACAGTAGCAAAAGATCCACCTCAAATAGAACCACCTACTTCAGAGAGTGAAAGTCCTTCAGCTCCACTAACTAAAGATGAGGAAGTGCAACCAGCTCCTAATACAAGTGGGAGTATCACCAGCTTAGATGTACAACCAGATCTTGCTTCTCCTCAAGAGACAAAAGCAACTATATCACCAGCTCTTGTAAAACAGCTCCGTGAAGAGTCAGGAGCTGGAATGATGGACTGCAAAAAGGCTTTGTTAGAGTCTGGAGGAGACATTGTCAAAGCCCAGGAGTTCCTTAGAAAGAAAGGCTTAGCAAGTGCAGACAAGAAAGCCAGCAGGGCCACTGCTGAAGGGAGAATAGGTTCATACATTCACGATAGCAGGATTGGTATCTTGCTGGAGGTAAACTGTGAGACAGATTTTGTTTCCCGGGGTGACATTTTCAAGGAGCTGGTTGATGATCTAGCTATGCAAGCAGCTGCATGCCCTCAAGTACAGTATGTTACTACAGAAGACGTTCCGGAAGAGTTTATAAACAAGGAAAGAGAGCTTGAGATGCAGAAAGAAGATCTTTTGTCAAAGCCAGAGCAGATTAGATCAAAGATTGTTGACGGGAGGATAAGGAAGAGGCTCGAGGAGCTGGCATTGCTTGAGCAGCCCTACATCAAGAATGATAAGGTGGTGGTGAAGGACTGGGTGAAGCAAACCATTGCAACCATTGGGGAAAACATAAAAGTCAAGAGGTTTGTGCGTTTCAATCTGGGAGAAGGCTTGGAAAAACGGAGTCAGGATTTTGCAGCTGAAGTGGCTGCTCAAACTGCAGCAAAAAAGGTGCCCACAGCAGGGAAAGAACAGCCTGCTGAAGTTGAAGCAAAGGAAACAGTTCAGAAGGCACCAACTGTAGCTATCTCAGCTGCTTTGGTTAAACAACTAAGGGAAGAAACTGGAGCGGGAATGATGGACTGCAAGAAAGCTCTCTCTGAAACTGGAGGGGATATAGAGAAGGCACAAGAGTACCTCAGAAAGAAAGGTCTTTCTTCTGCTGAAAAGAAATCCAGCCGGCTTGTTGCTGAAGGCAGGATAGGATCTTATATTCATGATGCTCGCATTGGAGTTCTGATTGAAGTAAACTCAGAGACTGATTTTGTTGGTAGAAGTGAAAAATTCAAGGAGTTGGTTGATGACCTGGCAATGCAAGTTGCGGCCTGCCCACAGGTGCAATTTGTATCTGTCGAAGATATTCCAGAGAGTACTGTAAAGAAGGAAAAAGAGCTTGAGATGCAGAGGGAGGACCTTCTGTCAAAACCTGAAAACATCAGGGAGAGAATCGTGGAGGGAAGGATCTCAAAGAGGCTTGGGGAGCTTGCTTTGTTGGAGCAACCTTTTATCAAAGATGATAGTATATTGGTGAAGGATCTGGTGAAGCAGACGATTGCTGCTCTTGGGGAAAACATAAAAGTTAGAAGGTTTGTTCGGTTCACTCTTGGGGGGACAGTAGAGGGTGCGAAATCAGAAGTTGAAGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF_TS S1

Protein Analysis

1061

Amino Acids

115.82

Weight (kDa)

4.9

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1_RRP5 PF23459 168 - 233 3.4e-06 RRP5 S1 domain
S1 PF00575 169 - 239 1.9e-15 S1 RNA binding domain
S1 PF00575 272 - 337 6.3e-07 S1 RNA binding domain
EF_TS PF00889 666 - 808 4.3e-28 Elongation factor TS
EF_TS PF00889 909 - 1048 1.5e-28 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29060 AT4G29060
fragaria_vesca FvH4_1g05650
malus_domestica MD02G1057500.v1.1 MD15G1191900.v1.1
prunus_persica Prupe.7G225300_v2.0.a1
pyrus_communis pycom02g04660
rosa_chinensis RchiOBHm_Chr2g0091281
rosa_laevigata RLG00000016202
rosa_multiflora Rmu_sc0002270.1_g000032
rosa_roxburghii Rroxscaffold_2G00150270
rosa_rugosa Rorug02G0015600 Rorug02G0015700 Rorug02G0015800
rosa_samantha Rh2AG061000 Rh2BG060000 Rh2CG062000 Rh2DG060500
rosa_wichuraiana Rw2G004880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2189
AccB1I GGYRCC 2 cut(s) 2488, 2548
AccBSI CCGCTC 1 cut(s) 2606
AccI GTMKAC 1 cut(s) 1662
AccIII TCCGGA 1 cut(s) 2176
AciI CCGC 5 cut(s) 112, 137, 697, 2606, 2858
AclWI GGATC 5 cut(s) 760, 1676, 2749, 3005, 3078
AcoI YGGCCR 1 cut(s) 973
AcsI RAATTY 3 cut(s) 853, 1494, 2819
AcuI CTGAAG 9 cut(s) 381, 1527, 1659, 1692, 1710, 2025, 2478, 2538, 2748
AfaI GTAC 7 cut(s) 177, 591, 1204, 1800, 2151, 2672, 2908
AfiI CCNNNNNNNGG 4 cut(s) 779, 1129, 2498, 2499
AflII CTTAAG 1 cut(s) 31
AflIII ACRYGT 1 cut(s) 592
AhdI GACNNNNNGTC 1 cut(s) 2953
AhlI ACTAGT 2 cut(s) 395, 458
AjnI CCWGG 7 cut(s) 505, 585, 975, 1110, 1404, 1941, 2841
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AloI GAACNNNNNNTCC 4 cut(s) 62, 94, 2002, 2034
Alw21I GWGCWC 2 cut(s) 123, 1345
Alw26I GTCTC 8 cut(s) 683, 950, 1309, 1551, 1820, 1920, 2063, 2786
AlwI GGATC 5 cut(s) 760, 1676, 2749, 3005, 3078
AlwNI CAGNNNCTG 4 cut(s) 311, 1637, 1883, 2795
Ama87I CYCGRG 2 cut(s) 2085, 2288
Aor13HI TCCGGA 1 cut(s) 2176
AoxI GGCC 5 cut(s) 213, 583, 973, 1995, 2859
ApoI RAATTY 3 cut(s) 853, 1494, 2819
Asp700I GAANNNNTTC 6 cut(s) 808, 1047, 2017, 2172, 2538, 2895
AspLEI GCGC 1 cut(s) 512
AspS9I GGNCC 4 cut(s) 213, 770, 1995, 2947
AsuC2I CCSGG 3 cut(s) 212, 2086, 2087
AvaI CYCGRG 2 cut(s) 2085, 2288
AvaII GGWCC 2 cut(s) 770, 2947
AxyI CCTNAGG 1 cut(s) 888
BaeGI GKGCMC 2 cut(s) 845, 2493
BaeI ACNNNNGTAYC 4 cut(s) 603, 636, 2654, 2687
BalI TGGCCA 1 cut(s) 975
BanI GGYRCC 2 cut(s) 2488, 2548
BanII GRGCYC 2 cut(s) 44, 123
BbsI GAAGAC 2 cut(s) 1407, 2175
Bbv12I GWGCWC 2 cut(s) 123, 1345
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 7 cut(s) 507, 587, 977, 1112, 1406, 1943, 2843
BcnI CCSGG 3 cut(s) 212, 2086, 2087
BcoDI GTCTC 8 cut(s) 683, 950, 1309, 1551, 1820, 1920, 2063, 2786
BcuI ACTAGT 2 cut(s) 395, 458
BfaI CTAG 7 cut(s) 282, 396, 459, 1422, 1439, 1593, 2120
BfmI CTRYAG 3 cut(s) 2163, 2475, 2556
BfrI CTTAAG 1 cut(s) 31
BglI GCCNNNNNGGC 1 cut(s) 1994
BglII AGATCT 2 cut(s) 1807, 2224
BlpI GCTNAGC 1 cut(s) 1966
BmcAI AGTACT 1 cut(s) 2908
Bme18I GGWCC 2 cut(s) 770, 2947
BmeRI GACNNNNNGTC 1 cut(s) 2953
BmeT110I CYCGRG 2 cut(s) 2085, 2288
BmgT120I GGNCC 4 cut(s) 213, 770, 1995, 2947
BmiI GGNNCC 5 cut(s) 504, 1369, 1503, 2490, 2550
BmrI ACTGGG 1 cut(s) 2359
BmsI GCATC 6 cut(s) 91, 373, 718, 2202, 2746, 2925
BmuI ACTGGG 1 cut(s) 2359
BpiI GAAGAC 2 cut(s) 1407, 2175
BpmI CTGGAG 4 cut(s) 1941, 2075, 2622, 2664
Bpu10I CCTNAGC 1 cut(s) 822
Bpu1102I GCTNAGC 1 cut(s) 1966
BpuEI CTTGAG 6 cut(s) 1059, 1806, 2130, 2228, 2327, 2951
BpuMI CCSGG 3 cut(s) 212, 2086, 2087
BsaBI GATNNNNATC 2 cut(s) 1578, 2045
BsaI GGTCTC 1 cut(s) 950
BsaJI CCNNGG 5 cut(s) 918, 1314, 1941, 2085, 2086
BsaWI WCCGGW 2 cut(s) 734, 2176
BsaXI ACNNNNNCTCC 8 cut(s) 754, 784, 1535, 1565, 1917, 1947, 2002, 2032
Bsc4I CCNNNNNNNGG 4 cut(s) 779, 1129, 2498, 2499
Bse118I RCCGGY 1 cut(s) 2715
Bse1I ACTGG 5 cut(s) 429, 1371, 2354, 2605, 2647
Bse21I CCTNAGG 1 cut(s) 888
Bse3DI GCAATG 4 cut(s) 1613, 2300, 2364, 2853
Bse8I GATNNNNATC 2 cut(s) 1578, 2045
BseAI TCCGGA 1 cut(s) 2176
BseBI CCWGG 7 cut(s) 507, 587, 977, 1112, 1406, 1943, 2843
BseDI CCNNGG 5 cut(s) 918, 1314, 1941, 2085, 2086
BseGI GGATG 3 cut(s) 279, 1083, 1456
BseJI GATNNNNATC 2 cut(s) 1578, 2045
BseLI CCNNNNNNNGG 4 cut(s) 779, 1129, 2498, 2499
BseMI GCAATG 4 cut(s) 1613, 2300, 2364, 2853
BseMII CTCAG 8 cut(s) 452, 596, 879, 926, 1227, 2580, 2689, 2802
BseNI ACTGG 5 cut(s) 429, 1371, 2354, 2605, 2647
BseRI GAGGAG 5 cut(s) 113, 509, 1809, 1938, 2306
BseSI GKGCMC 2 cut(s) 845, 2493
BseYI CCCAGC 1 cut(s) 37
BsgI GTGCAG 2 cut(s) 1476, 1995
BshFI GGCC 5 cut(s) 215, 585, 975, 1997, 2861
BshNI GGYRCC 2 cut(s) 2488, 2548
BsiHKAI GWGCWC 2 cut(s) 123, 1345
BsiHKCI CYCGRG 2 cut(s) 2085, 2288
BsiSI CCGG 5 cut(s) 211, 735, 2086, 2177, 2716
BslFI GGGAC 3 cut(s) 322, 1048, 3160
BslI CCNNNNNNNGG 4 cut(s) 779, 1129, 2498, 2499
BsmAI GTCTC 8 cut(s) 683, 950, 1309, 1551, 1820, 1920, 2063, 2786
BsmFI GGGAC 3 cut(s) 322, 1048, 3160
BsnI GGCC 5 cut(s) 215, 585, 975, 1997, 2861
Bso31I GGTCTC 1 cut(s) 950
BsoBI CYCGRG 2 cut(s) 2085, 2288
Bsp1286I GDGCHC 5 cut(s) 44, 123, 845, 1345, 2493
Bsp13I TCCGGA 1 cut(s) 2176
Bsp1407I TGTACA 1 cut(s) 1798
Bsp1720I GCTNAGC 1 cut(s) 1966
BspACI CCGC 5 cut(s) 112, 137, 697, 2606, 2858
BspANI GGCC 5 cut(s) 215, 585, 975, 1997, 2861
BspCNI CTCAG 8 cut(s) 451, 597, 880, 925, 1228, 2579, 2688, 2801
BspEI TCCGGA 1 cut(s) 2176
BspHI TCATGA 2 cut(s) 1255, 2752
BspLI GGNNCC 5 cut(s) 504, 1369, 1503, 2490, 2550
BspMAI CTGCAG 1 cut(s) 2479
BspPI GGATC 5 cut(s) 760, 1676, 2749, 3005, 3078
BspT107I GGYRCC 2 cut(s) 2488, 2548
BspTI CTTAAG 1 cut(s) 31
BspTNI GGTCTC 1 cut(s) 950
BsrBI CCGCTC 1 cut(s) 2606
BsrDI GCAATG 4 cut(s) 1613, 2300, 2364, 2853
BsrFI RCCGGY 1 cut(s) 2715
BsrGI TGTACA 1 cut(s) 1798
BsrI ACTGG 5 cut(s) 429, 1371, 2354, 2605, 2647
BssAI RCCGGY 1 cut(s) 2715
BssECI CCNNGG 5 cut(s) 918, 1314, 1941, 2085, 2086
Bst2UI CCWGG 7 cut(s) 507, 587, 977, 1112, 1406, 1943, 2843
Bst6I CTCTTC 7 cut(s) 888, 1312, 1473, 1635, 1866, 2175, 2276
BstAFI CTTAAG 1 cut(s) 31
BstAPI GCANNNNNTGC 1 cut(s) 449
BstAUI TGTACA 1 cut(s) 1798
BstDSI CCRYGG 1 cut(s) 1314
BstF5I GGATG 3 cut(s) 279, 1083, 1456
BstHHI GCGC 1 cut(s) 512
BstMAI GTCTC 8 cut(s) 683, 950, 1309, 1551, 1820, 1920, 2063, 2786
BstNI CCWGG 7 cut(s) 507, 587, 977, 1112, 1406, 1943, 2843
BstNSI RCATGY 2 cut(s) 596, 2142
BstSFI CTRYAG 3 cut(s) 2163, 2475, 2556
BstSLI GKGCMC 2 cut(s) 845, 2493
BstV2I GAAGAC 2 cut(s) 1407, 2175
BstX2I RGATCY 7 cut(s) 752, 1681, 1807, 2224, 2741, 2997, 3070
BstXI CCANNNNNNTGG 1 cut(s) 548
BstYI RGATCY 7 cut(s) 752, 1681, 1807, 2224, 2741, 2997, 3070
Bsu36I CCTNAGG 1 cut(s) 888
BsuRI GGCC 5 cut(s) 215, 585, 975, 1997, 2861
BtgI CCRYGG 1 cut(s) 1314
BtsCI GGATG 3 cut(s) 279, 1083, 1456
BtsI GCAGTG 2 cut(s) 327, 1998
BtsIMutI CAGTG 3 cut(s) 327, 1378, 1998
CaiI CAGNNNCTG 4 cut(s) 311, 1637, 1883, 2795
CciI TCATGA 2 cut(s) 1255, 2752
CfoI GCGC 1 cut(s) 512
Cfr10I RCCGGY 1 cut(s) 2715
Cfr13I GGNCC 4 cut(s) 213, 770, 1995, 2947
Cfr9I CCCGGG 1 cut(s) 2085
CseI GACGC 1 cut(s) 428
Csp6I GTAC 7 cut(s) 176, 590, 1203, 1799, 2150, 2671, 2907
CviAII CATG 4 cut(s) 593, 1256, 2139, 2753
CviQI GTAC 7 cut(s) 176, 590, 1203, 1799, 2150, 2671, 2907
DriI GACNNNNNGTC 1 cut(s) 2953
EaeI YGGCCR 1 cut(s) 973
Eam1104I CTCTTC 7 cut(s) 888, 1312, 1473, 1635, 1866, 2175, 2276
Eam1105I GACNNNNNGTC 1 cut(s) 2953
EarI CTCTTC 7 cut(s) 888, 1312, 1473, 1635, 1866, 2175, 2276
Ecl136II GAGCTC 1 cut(s) 121
Eco24I GRGCYC 2 cut(s) 44, 123
Eco31I GGTCTC 1 cut(s) 950
Eco32I GATATC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 770, 2947
Eco53kI GAGCTC 1 cut(s) 121
Eco57I CTGAAG 9 cut(s) 381, 1527, 1659, 1692, 1710, 2025, 2478, 2538, 2748
Eco81I CCTNAGG 1 cut(s) 888
Eco88I CYCGRG 2 cut(s) 2085, 2288
EcoICRI GAGCTC 1 cut(s) 121
EcoO109I RGGNCCY 1 cut(s) 2947
EcoRII CCWGG 7 cut(s) 505, 585, 975, 1110, 1404, 1941, 2841
EcoRV GATATC 1 cut(s) 702
EcoT38I GRGCYC 2 cut(s) 44, 123
FaeI CATG 4 cut(s) 596, 1259, 2142, 2756
FalI AAGNNNNNCTT 6 cut(s) 179, 211, 1949, 1981, 2211, 2243
FaqI GGGAC 3 cut(s) 322, 1048, 3160
FatI CATG 4 cut(s) 592, 1255, 2138, 2752
FauI CCCGC 2 cut(s) 105, 2599
FblI GTMKAC 1 cut(s) 1662
FokI GGATG 3 cut(s) 286, 1070, 1463
FriOI GRGCYC 2 cut(s) 44, 123
FspBI CTAG 7 cut(s) 282, 396, 459, 1422, 1439, 1593, 2120
GlaI GCGC 1 cut(s) 511
GsaI CCCAGC 1 cut(s) 41
GsuI CTGGAG 4 cut(s) 1941, 2075, 2622, 2664
HaeIII GGCC 5 cut(s) 215, 585, 975, 1997, 2861
HapII CCGG 5 cut(s) 211, 735, 2086, 2177, 2716
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 512
Hin1II CATG 4 cut(s) 596, 1259, 2142, 2756
Hin6I GCGC 1 cut(s) 510
HinP1I GCGC 1 cut(s) 510
HincII GTYRAC 2 cut(s) 1554, 2266
HindII GTYRAC 2 cut(s) 1554, 2266
HindIII AAGCTT 2 cut(s) 351, 363
HpaII CCGG 5 cut(s) 211, 735, 2086, 2177, 2716
Hpy166II GTNNAC 7 cut(s) 1318, 1554, 1663, 2062, 2266, 2785, 3135
Hpy8I GTNNAC 7 cut(s) 1318, 1554, 1663, 2062, 2266, 2785, 3135
HpyCH4IV ACGT 3 cut(s) 315, 1388, 2172
HpySE526I ACGT 3 cut(s) 315, 1388, 2172
Hsp92II CATG 4 cut(s) 596, 1259, 2142, 2756
HspAI GCGC 1 cut(s) 510
Kpn2I TCCGGA 1 cut(s) 2176
KroI GCCGGC 1 cut(s) 2715
KroNI GCCGGC 1 cut(s) 2717
LweI GCATC 6 cut(s) 91, 373, 718, 2202, 2746, 2925
MaeI CTAG 7 cut(s) 282, 396, 459, 1422, 1439, 1593, 2120
MaeII ACGT 3 cut(s) 315, 1388, 2172
MaeIII GTNAC 5 cut(s) 477, 664, 1018, 2090, 2158
MbiI CCGCTC 1 cut(s) 2606
MflI RGATCY 7 cut(s) 752, 1681, 1807, 2224, 2741, 2997, 3070
MhlI GDGCHC 5 cut(s) 44, 123, 845, 1345, 2493
MlsI TGGCCA 1 cut(s) 975
MluCI AATT 6 cut(s) 248, 853, 1494, 1536, 2819, 2876
MluNI TGGCCA 1 cut(s) 975
MlyI GAGTC 8 cut(s) 329, 1070, 1089, 1253, 1553, 1883, 1925, 2449
MmeI TCCRAC 3 cut(s) 435, 1345, 3009
Mox20I TGGCCA 1 cut(s) 975
MroI TCCGGA 1 cut(s) 2176
MroNI GCCGGC 1 cut(s) 2715
MroXI GAANNNNTTC 6 cut(s) 808, 1047, 2017, 2172, 2538, 2895
MscI TGGCCA 1 cut(s) 975
MseI TTAA 6 cut(s) 32, 258, 486, 984, 1227, 2580
MslI CAYNNNNRTG 1 cut(s) 131
Msp20I TGGCCA 1 cut(s) 975
MspA1I CMGCKG 5 cut(s) 389, 604, 2135, 2456, 2570
MspCI CTTAAG 1 cut(s) 31
MspI CCGG 5 cut(s) 211, 735, 2086, 2177, 2716
MvaI CCWGG 7 cut(s) 507, 587, 977, 1112, 1406, 1943, 2843
NaeI GCCGGC 1 cut(s) 2717
NciI CCSGG 3 cut(s) 212, 2086, 2087
NgoMIV GCCGGC 1 cut(s) 2715
NlaIII CATG 4 cut(s) 596, 1259, 2142, 2756
NlaIV GGNNCC 5 cut(s) 504, 1369, 1503, 2490, 2550
NmuCI GTSAC 3 cut(s) 477, 664, 2090
NspI RCATGY 2 cut(s) 596, 2142
PaeI GCATGC 1 cut(s) 2142
PaeR7I CTCGAG 1 cut(s) 2288
PagI TCATGA 2 cut(s) 1255, 2752
PciI ACATGT 1 cut(s) 592
PdiI GCCGGC 1 cut(s) 2717
PdmI GAANNNNTTC 6 cut(s) 808, 1047, 2017, 2172, 2538, 2895
PfeI GAWTC 5 cut(s) 1211, 1529, 1574, 1586, 2982
PflFI GACNNNGTC 1 cut(s) 1931
PleI GAGTC 8 cut(s) 329, 1069, 1088, 1252, 1552, 1882, 1924, 2448
PpsI GAGTC 8 cut(s) 329, 1069, 1088, 1252, 1552, 1882, 1924, 2448
PpuMI RGGWCCY 1 cut(s) 2947
PscI ACATGT 1 cut(s) 592
PsiI TTATAA 1 cut(s) 2189
Psp124BI GAGCTC 1 cut(s) 123
Psp5II RGGWCCY 1 cut(s) 2947
Psp6I CCWGG 7 cut(s) 505, 585, 975, 1110, 1404, 1941, 2841
PspFI CCCAGC 1 cut(s) 37
PspGI CCWGG 7 cut(s) 505, 585, 975, 1110, 1404, 1941, 2841
PspN4I GGNNCC 5 cut(s) 504, 1369, 1503, 2490, 2550
PspPI GGNCC 4 cut(s) 213, 770, 1995, 2947
PspPPI RGGWCCY 1 cut(s) 2947
PspXI VCTCGAGB 1 cut(s) 2288
PstI CTGCAG 1 cut(s) 2479
PstNI CAGNNNCTG 4 cut(s) 311, 1637, 1883, 2795
PsuI RGATCY 7 cut(s) 752, 1681, 1807, 2224, 2741, 2997, 3070
PsyI GACNNNGTC 1 cut(s) 1931
PvuII CAGCTG 5 cut(s) 389, 604, 2135, 2456, 2570
RsaI GTAC 7 cut(s) 177, 591, 1204, 1800, 2151, 2672, 2908
RsaNI GTAC 7 cut(s) 176, 590, 1203, 1799, 2150, 2671, 2907
RseI CAYNNNNRTG 1 cut(s) 131
SacI GAGCTC 1 cut(s) 123
SaqAI TTAA 6 cut(s) 32, 258, 486, 984, 1227, 2580
Sau96I GGNCC 4 cut(s) 213, 770, 1995, 2947
ScaI AGTACT 1 cut(s) 2908
SchI GAGTC 8 cut(s) 329, 1070, 1089, 1253, 1553, 1883, 1925, 2449
SduI GDGCHC 5 cut(s) 44, 123, 845, 1345, 2493
SfaNI GCATC 6 cut(s) 91, 373, 718, 2202, 2746, 2925
SfcI CTRYAG 3 cut(s) 2163, 2475, 2556
Sfr274I CTCGAG 1 cut(s) 2288
SinI GGWCC 2 cut(s) 770, 2947
SlaI CTCGAG 1 cut(s) 2288
SmaI CCCGGG 1 cut(s) 2087
SmiMI CAYNNNNRTG 1 cut(s) 131
SmlI CTYRAG 8 cut(s) 31, 1074, 1821, 2145, 2207, 2288, 2306, 2930
SmoI CTYRAG 8 cut(s) 31, 1074, 1821, 2145, 2207, 2288, 2306, 2930
SpeI ACTAGT 2 cut(s) 395, 458
SphI GCATGC 1 cut(s) 2142
Sse9I AATT 6 cut(s) 248, 853, 1494, 1536, 2819, 2876
SsiI CCGC 5 cut(s) 112, 137, 697, 2606, 2858
SspI AATATT 1 cut(s) 1252
SspMI CTAG 7 cut(s) 282, 396, 459, 1422, 1439, 1593, 2120
SstI GAGCTC 1 cut(s) 123
TaiI ACGT 3 cut(s) 318, 1391, 2175
TaqI TCGA 6 cut(s) 61, 99, 150, 218, 2289, 2889
TasI AATT 6 cut(s) 248, 853, 1494, 1536, 2819, 2876
TatI WGTACW 4 cut(s) 1202, 1798, 2149, 2906
TauI GCSGC 1 cut(s) 2861
TfiI GAWTC 5 cut(s) 1211, 1529, 1574, 1586, 2982
Tru1I TTAA 6 cut(s) 32, 258, 486, 984, 1227, 2580
Tru9I TTAA 6 cut(s) 32, 258, 486, 984, 1227, 2580
TscAI CASTG 3 cut(s) 327, 1378, 2005
TseFI GTSAC 3 cut(s) 477, 664, 2090
Tsp45I GTSAC 3 cut(s) 477, 664, 2090
TspDTI ATGAA 9 cut(s) 290, 547, 945, 1046, 1244, 1245, 1272, 2010, 2741
TspGWI ACGGA 4 cut(s) 980, 1539, 1856, 2452
TspMI CCCGGG 1 cut(s) 2085
TspRI CASTG 3 cut(s) 327, 1378, 2005
Tth111I GACNNNGTC 1 cut(s) 1931
Vha464I CTTAAG 1 cut(s) 31
VpaK11BI GGWCC 2 cut(s) 770, 2947
XapI RAATTY 3 cut(s) 853, 1494, 2819
XbaI TCTAGA 2 cut(s) 281, 1438
XceI RCATGY 2 cut(s) 596, 2142
XhoI CTCGAG 1 cut(s) 2288
XmaI CCCGGG 1 cut(s) 2085
XmiI GTMKAC 1 cut(s) 1662
XmnI GAANNNNTTC 6 cut(s) 808, 1047, 2017, 2172, 2538, 2895
XspI CTAG 7 cut(s) 282, 396, 459, 1422, 1439, 1593, 2120
ZrmI AGTACT 1 cut(s) 2908
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.