MD15G1191900.v1.1

Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0012242
Physical Location & Seq
Reverse (-)
15114072 .. 15119260
5189 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1191900.v1.1.491

Sequence Viewer

Length: 3135 bp
ATGTTAGCAGCAGACTCAACTTCTATTTTTCAAGTCAACATGACGCCTGTAATTCCGTATTCTATAAGCAATGTCTCGCATATTCCTGGAACTGCCTTTACTGCAAGAAAGAACAATTGTTTAACAAAATTCAGTTTTTCAAGGAATTCTGCAAAACATGCTCTATCCCCACGGAGTTTTCTTTTACCTTTTTCGACCTCTATCAAATCATTTGCATTGTACCACAGCAGATGCCCCGTATATCATAGATCTAGAATCCATGTATCTGCCACAGGAACTGATGTGGCAGTTGAGGAAGCAGACTCACCAGTTGCAGATGCAGCTTCTAGTGAAGCCAAATCCTCTGATGATAGTCCTGGCCCATCTCAAGATTCTCAACCAAAGCGTTCAAAACCTGTTAGGAAAAGTGAGATGCCACCTGTGAAGAATGAAGAGCTTGTTGTCGGTGCAACTTTTACGGGGAAAGTAAGATCAATCCAGCCATTTGGTGCTTTTATTGACTTTGGAGCTTTCACAGATGGCCTCATACATGTTTCACAATTGAGTGATAGTTACGTTAAGGATGTTGGAAGCATTGTTTCTGTTGGGCAAGAGGTGAAAGTGACATTAGTTGAAGCCAATCCAGAGACTGGGCGGATCTCTCTCACTATGCGTGAACGTGACAATGGCAGTAAGTTGCAGCAAAGGAAAGATGCTTCTGCTGGTAGTGATAGGGGTGGACCTGGTAGAAGGAGTGGCCCAAAGAAAGGCGAAGGGAAAAAAGAGGTGAGGAAAACTACGAAGTTTGAAAAAGGGCAGGACCTAGTGGGCACAGTAAAGAATTTTGCCAGGGCTGGTGCTTTTATATCTCTTCCTGAGGGAGAGGAAGGATTCCTGCCTACATCGGAGGAACCTGATGATGGATTTGCAAACGTCATGGGGGAGACCTCCTTGCAGCTTGGCCAAGAAATAAATGTCCGTGTCCTGCGTACTACAAGAGGACAAGTAACCTTGACAATGAAGAAAGAAGAAGATATTCTAAAGTCGGACTCACAGGTCAGTCAAGGAGTAATCCACACTGCAACAAATCCATTCCTTCTGGCTTTTCGCCAAAACAAGGATATTGCTTCATTTTTGGACGAAAGAGAGAAAATAGAAAAAGCAGCTAAAGCCATTGCAAGCAGTGAGTCCAGTATTCCTGAAGTGTTGGATGAACATACAACCAGTGACGAGGGAACTCTTGGTGTCCTTTCAGCGGTGGATGAAACAGTTGAAAATGGTGCTCCTTCAGAGGACCAGGAAAGCCCAGTTTCTAGTACAATTGAAACTCTAGAAACCACAGAACAACCCATTGAAAGAGAAGAGGTGAGTTCTGATATCTTGGCTCCTGAAGGGAGTACATTTACCATGGATGGAGTGGAAAATGCAATTGCTGGTTCGTCTAGTGAAATAGCTAATTACACGTCGACTTCAGAAGTTCCAACAGGCGAAGAGGTTATAGAGCCTCAAGCGGATGATACCATAGAAAAAGGTGAACTGCAACCACCTACTTCAGAGAGAGAAATTCCTTCTGCTGCACTAACTGAAGAACCAAAAGAAAGTGAGGCCACCAAAGTTGTAGAAGACCTAGCTGACAACATTACAGAGGAAGCTCAGATTCAAACATCTGCTGCTGAGAGTGAATTGCCTTCTATCTCACAAGTGGAAGGTGACAAAGTGGAAAGTGCCCCCAAGAAGAATGGCAGTGCTTCTGATTCAAATCGACAATCTGACAATCCTTCCCCGAAGGAAAGAGAAATTAAAGCAATTATATCACCAGCCCTTGTAAAGCAGCTGCGTGAAGAAACAGGAGCTGGAATGATGGATTGCAAAAATGCTTTGTCAGAGACTGGCGGGGACATTGTTAAAGCTACGGAGTTCCTTAGAAAGAAAGGTTTAGCTAGTGCAGAAAAGAAAGCCAGCAGAGCCACTGCTGAAGGAAGAATAGGTTCATACATTCATGATAGCAGGATAGGTATCTTGCTAGAGGTAAACTGTGAGACAGATTTTGTTTCTCGGGGTGACATTTTCAAGGAGCTGGTTGATGATTTAGCCATGCAAGTGGCTGCATGCCCTCAAGTACAGTACCTTGCTACAGAAGATGTTCCTGAAGAGCTTGTTAACAAGGAAAGAGCGATTGAGATGCAGAAAGAAGATCTTTTGTCAAAGCCGGAGCAGATTAGGTCAAAGATTGTTGACGGGAGGATAAGGAAGAGGCTTGAGGAGCTGGCATTGCTTGAGCAGCCTTTCATCAAGAATGATAAGGTGGTGGTGAAGGACTTGGTCAAACAAACCATTTCAACTATTGGAGAAAACATAAAAGTGAAGAGGTTTGTCCGCTACAATCTTGGAGAGGGCTTGGAGAAGAAGAGTCAGGATTTTGCTGCTGAGGTGGCTGCCCAAACTGCAGCAAAACCAGTGCCCGCGGAAGTAAAAGAGCAGCCTGCTGCAGTGGAAGTCAAGGAAACTGTTGAGAAGGCACCAACTGTAGCCGTCTCTGCCGCTTTGGTTAAACAACTACGTGATGAAACTGGAGCAGGGATGATGGACTGCAAGAAAGCTCTCTCCGAAACTGGAGGGGACCTTGAGATGGCACAAGAGTACCTGCGGAAGAAGGGTCTTTCTTCTGCTGAAAAGAAATCCAGCAGGCTTGCAGCTGAAGGCAGGATTGGGTCCTACATTCATGACGCTCTCATTGGAGTTCTGATTGAAGTCAACTGCGAGACTGACTTTGTTGGTAGAAGTGAAAATTTCAAGGGGTTGGTTGATGATCTGGCAATGCAAGTCGTGGCCTGCCCTCAGGTGCAATATGTATCCATCGAAGACATTCCAGAGAGCATTGTAAACAAGGAAAAAGAGCTTGAGAGGCAGAGGGAGGACCTTCTGTCCAAACCCGAGAACATTAGGGAGAGAATTGTGGAGGGGAGGATCTCAAAGAGGCTTGGGGAGCTGGCTCTTTTAGAGCAACCTTTCATTAAAGATGATAGTCTTTTGGTGAAAGACCTAGTGAAGCAGACTGTTGCTGCTCTGGGTGAGAACATAAAAGTTCGCAGGTTTGTTCGGTTCACTCTTGGGGAGTCAGTTGAGACAGTTGAAGATGCAGGAGCTGAAGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF_TS S1

Protein Analysis

1045

Amino Acids

113.43

Weight (kDa)

4.95

Isoelectric Point (pI)

49.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
S1 PF00575 148 - 218 2.8e-14 S1 RNA binding domain
S1 PF00575 261 - 325 1.8e-06 S1 RNA binding domain
EF_TS PF00889 649 - 791 1.7e-28 Elongation factor TS
EF_TS PF00889 906 - 936 2e-06 Elongation factor TS
EF_TS PF00889 929 - 1032 5.1e-22 Elongation factor TS
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012242)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29060 AT4G29060
fragaria_vesca FvH4_1g05650
malus_domestica MD02G1057500.v1.1 MD15G1191900.v1.1
prunus_persica Prupe.7G225300_v2.0.a1
pyrus_communis pycom02g04660
rosa_chinensis RchiOBHm_Chr2g0091281
rosa_laevigata RLG00000016202
rosa_multiflora Rmu_sc0002270.1_g000032
rosa_roxburghii Rroxscaffold_2G00150270
rosa_rugosa Rorug02G0015600 Rorug02G0015700 Rorug02G0015800
rosa_samantha Rh2AG061000 Rh2BG060000 Rh2CG062000 Rh2DG060500
rosa_wichuraiana Rw2G004880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1034
Acc36I ACCTGC 2 cut(s) 2631, 3060
AccB1I GGYRCC 1 cut(s) 2497
AccB7I CCANNNNNTGG 1 cut(s) 629
AccI GTMKAC 1 cut(s) 1445
AccII CGCG 1 cut(s) 2444
AciI CCGC 9 cut(s) 634, 1235, 1490, 1872, 2356, 2442, 2444, 2520, 2626
AclWI GGATC 2 cut(s) 644, 2954
AcoI YGGCCR 1 cut(s) 940
AcsI RAATTY 5 cut(s) 128, 145, 820, 1542, 2767
AcuI CTGAAG 9 cut(s) 1200, 1251, 1389, 1434, 1515, 1584, 1974, 2148, 2697
AcyI GRCGYC 1 cut(s) 44
AfaI GTAC 7 cut(s) 221, 970, 1297, 1378, 2100, 2105, 2621
AfiI CCNNNNNNNGG 6 cut(s) 629, 746, 899, 1096, 1234, 2608
AflIII ACRYGT 2 cut(s) 529, 1440
AhdI GACNNNNNGTC 1 cut(s) 2902
AjiI CACGTC 1 cut(s) 1443
AjnI CCWGG 5 cut(s) 85, 355, 721, 827, 1275
AjuI GAANNNNNNNTTGG 2 cut(s) 2670, 2702
Alw21I GWGCWC 1 cut(s) 1264
Alw26I GTCTC 8 cut(s) 79, 620, 917, 1859, 2012, 2518, 2735, 3098
AlwI GGATC 2 cut(s) 644, 2954
AlwNI CAGNNNCTG 5 cut(s) 278, 629, 1832, 1868, 3125
Ama87I CYCGRG 2 cut(s) 2034, 2912
AoxI GGCC 6 cut(s) 358, 520, 736, 940, 1584, 2808
ApoI RAATTY 5 cut(s) 128, 145, 820, 1542, 2767
Asp700I GAANNNNTTC 4 cut(s) 1014, 1966, 2121, 2844
AspS9I GGNCC 8 cut(s) 359, 719, 737, 799, 1273, 2599, 2691, 2896
AvaI CYCGRG 2 cut(s) 2034, 2912
AvaII GGWCC 6 cut(s) 719, 799, 1273, 2599, 2691, 2896
AxyI CCTNAGG 2 cut(s) 855, 2817
BaeGI GKGCMC 3 cut(s) 812, 1708, 2442
BaeI ACNNNNGTAYC 2 cut(s) 2603, 2636
BalI TGGCCA 1 cut(s) 942
BanI GGYRCC 1 cut(s) 2497
BbsI GAAGAC 2 cut(s) 1608, 2847
Bbv12I GWGCWC 1 cut(s) 1264
BbvCI CCTCAGC 1 cut(s) 2406
BccI CCATC 8 cut(s) 370, 512, 893, 1385, 1834, 2557, 2602, 2843
BceAI ACGGC 1 cut(s) 2495
BcgI CGANNNNNNTGC 2 cut(s) 2143, 2177
BciT130I CCWGG 5 cut(s) 87, 357, 723, 829, 1277
BciVI GTATCC 1 cut(s) 2842
BcoDI GTCTC 8 cut(s) 79, 620, 917, 1859, 2012, 2518, 2735, 3098
BfmI CTRYAG 4 cut(s) 2112, 2424, 2466, 2505
BfuAI ACCTGC 2 cut(s) 2631, 3060
BfuI GTATCC 1 cut(s) 2842
BglII AGATCT 2 cut(s) 248, 2173
Bme1390I CCNGG 5 cut(s) 87, 357, 723, 829, 1277
Bme18I GGWCC 6 cut(s) 719, 799, 1273, 2599, 2691, 2896
BmeRI GACNNNNNGTC 1 cut(s) 2902
BmeT110I CYCGRG 2 cut(s) 2034, 2912
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 8 cut(s) 359, 719, 737, 799, 1273, 2599, 2691, 2896
BmiI GGNNCC 5 cut(s) 891, 1365, 2499, 2600, 2692
BmrFI CCNGG 5 cut(s) 87, 357, 723, 829, 1277
BmrI ACTGGG 2 cut(s) 639, 1280
BmsI GCATC 6 cut(s) 221, 307, 402, 682, 2151, 3106
BmuI ACTGGG 2 cut(s) 639, 1280
BpiI GAAGAC 2 cut(s) 1608, 2847
BpmI CTGGAG 2 cut(s) 2571, 2613
Bpu10I CCTNAGC 1 cut(s) 2406
BpuEI CTTGAG 7 cut(s) 351, 1470, 2079, 2258, 2276, 2624, 2900
BsaAI YACGTR 1 cut(s) 2540
BsaBI GATNNNNATC 2 cut(s) 1737, 1994
BsaHI GRCGYC 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 917
BsaJI CCNNGG 4 cut(s) 170, 828, 1386, 2442
Bsc4I CCNNNNNNNGG 6 cut(s) 629, 746, 899, 1096, 1234, 2608
Bse1I ACTGG 9 cut(s) 308, 634, 1170, 1203, 1286, 1873, 2435, 2554, 2596
Bse21I CCTNAGG 2 cut(s) 855, 2817
Bse3DI GCAATG 4 cut(s) 76, 1152, 2249, 2802
Bse8I GATNNNNATC 2 cut(s) 1737, 1994
BseBI CCWGG 5 cut(s) 87, 357, 723, 829, 1277
BseDI CCNNGG 4 cut(s) 170, 828, 1386, 2442
BseGI GGATG 6 cut(s) 568, 1195, 1246, 1396, 1498, 2565
BseJI GATNNNNATC 2 cut(s) 1737, 1994
BseLI CCNNNNNNNGG 6 cut(s) 629, 746, 899, 1096, 1234, 2608
BseMI GCAATG 4 cut(s) 76, 1152, 2249, 2802
BseMII CTCAG 5 cut(s) 846, 1644, 1646, 2397, 2831
BseNI ACTGG 9 cut(s) 308, 634, 1170, 1203, 1286, 1873, 2435, 2554, 2596
BseRI GAGGAG 1 cut(s) 2255
BseSI GKGCMC 3 cut(s) 812, 1708, 2442
BsgI GTGCAG 2 cut(s) 1539, 1944
Bsh1236I CGCG 1 cut(s) 2444
BshFI GGCC 6 cut(s) 360, 522, 738, 942, 1586, 2810
BshNI GGYRCC 1 cut(s) 2497
BsiHKAI GWGCWC 1 cut(s) 1264
BsiHKCI CYCGRG 2 cut(s) 2034, 2912
BsiSI CCGG 1 cut(s) 2189
BslFI GGGAC 2 cut(s) 1889, 2612
BslI CCNNNNNNNGG 6 cut(s) 629, 746, 899, 1096, 1234, 2608
BsmAI GTCTC 8 cut(s) 79, 620, 917, 1859, 2012, 2518, 2735, 3098
BsmBI CGTCTC 1 cut(s) 2518
BsmFI GGGAC 2 cut(s) 1889, 2612
BsnI GGCC 6 cut(s) 360, 522, 738, 942, 1586, 2810
Bso31I GGTCTC 1 cut(s) 917
BsoBI CYCGRG 2 cut(s) 2034, 2912
Bsp1286I GDGCHC 4 cut(s) 812, 1264, 1708, 2442
Bsp143I GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
Bsp19I CCATGG 1 cut(s) 1386
BspACI CCGC 9 cut(s) 634, 1235, 1490, 1872, 2356, 2442, 2444, 2520, 2626
BspANI GGCC 6 cut(s) 360, 522, 738, 942, 1586, 2810
BspCNI CTCAG 5 cut(s) 847, 1645, 1645, 2398, 2830
BspFNI CGCG 1 cut(s) 2444
BspHI TCATGA 2 cut(s) 1978, 2701
BspLI GGNNCC 5 cut(s) 891, 1365, 2499, 2600, 2692
BspMAI CTGCAG 2 cut(s) 2428, 2470
BspMI ACCTGC 2 cut(s) 2631, 3060
BspPI GGATC 2 cut(s) 644, 2954
BspQI GCTCTTC 2 cut(s) 426, 2124
BspT107I GGYRCC 1 cut(s) 2497
BspTNI GGTCTC 1 cut(s) 917
BsrDI GCAATG 4 cut(s) 76, 1152, 2249, 2802
BsrI ACTGG 9 cut(s) 308, 634, 1170, 1203, 1286, 1873, 2435, 2554, 2596
BssECI CCNNGG 4 cut(s) 170, 828, 1386, 2442
BssMI GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
BssNI GRCGYC 1 cut(s) 44
BssT1I CCWWGG 1 cut(s) 1386
Bst2UI CCWGG 5 cut(s) 87, 357, 723, 829, 1277
Bst4CI ACNGT 8 cut(s) 814, 1249, 2015, 2103, 2488, 2506, 3037, 3109
Bst6I CTCTTC 8 cut(s) 426, 855, 1335, 1464, 2124, 2225, 2339, 2381
BstACI GRCGYC 1 cut(s) 44
BstAPI GCANNNNNTGC 1 cut(s) 158
BstBAI YACGTR 1 cut(s) 2540
BstDEI CTNAG 6 cut(s) 855, 1632, 1653, 1901, 2406, 2817
BstDSI CCRYGG 3 cut(s) 170, 1386, 2442
BstF5I GGATG 6 cut(s) 568, 1195, 1246, 1396, 1498, 2565
BstFNI CGCG 1 cut(s) 2444
BstKTI GATC 6 cut(s) 251, 473, 639, 2176, 2791, 2949
BstMAI GTCTC 8 cut(s) 79, 620, 917, 1859, 2012, 2518, 2735, 3098
BstMBI GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
BstNI CCWGG 5 cut(s) 87, 357, 723, 829, 1277
BstNSI RCATGY 3 cut(s) 161, 533, 2091
BstSCI CCNGG 5 cut(s) 85, 355, 721, 827, 1275
BstSFI CTRYAG 4 cut(s) 2112, 2424, 2466, 2505
BstSLI GKGCMC 3 cut(s) 812, 1708, 2442
BstUI CGCG 1 cut(s) 2444
BstV2I GAAGAC 2 cut(s) 1608, 2847
BstX2I RGATCY 4 cut(s) 248, 636, 2173, 2946
BstXI CCANNNNNNTGG 2 cut(s) 485, 2080
BstYI RGATCY 4 cut(s) 248, 636, 2173, 2946
Bsu36I CCTNAGG 2 cut(s) 855, 2817
BsuI GTATCC 1 cut(s) 2842
BsuRI GGCC 6 cut(s) 360, 522, 738, 942, 1586, 2810
BtgI CCRYGG 3 cut(s) 170, 1386, 2442
BtrI CACGTC 1 cut(s) 1443
BtsCI GGATG 6 cut(s) 568, 1195, 1246, 1396, 1498, 2565
BtsI GCAGTG 5 cut(s) 1056, 1168, 1729, 1947, 2475
BtsIMutI CAGTG 7 cut(s) 1056, 1168, 1210, 1729, 1947, 2442, 2475
BveI ACCTGC 2 cut(s) 2631, 3060
CaiI CAGNNNCTG 5 cut(s) 278, 629, 1832, 1868, 3125
CciI TCATGA 2 cut(s) 1978, 2701
Cfr13I GGNCC 8 cut(s) 359, 719, 737, 799, 1273, 2599, 2691, 2896
Cfr42I CCGCGG 1 cut(s) 2445
CseI GACGC 2 cut(s) 52, 2714
CsiI ACCWGGT 1 cut(s) 721
Csp6I GTAC 7 cut(s) 220, 969, 1296, 1377, 2099, 2104, 2620
CspCI CAANNNNNGTGG 2 cut(s) 1576, 1611
CviQI GTAC 7 cut(s) 220, 969, 1296, 1377, 2099, 2104, 2620
DdeI CTNAG 6 cut(s) 855, 1632, 1653, 1901, 2406, 2817
DpnI GATC 6 cut(s) 250, 472, 638, 2175, 2790, 2948
DpnII GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
DrdI GACNNNNNNGTC 1 cut(s) 1034
DriI GACNNNNNGTC 1 cut(s) 2902
DseDI GACNNNNNNGTC 1 cut(s) 1034
EaeI YGGCCR 1 cut(s) 940
Eam1104I CTCTTC 8 cut(s) 426, 855, 1335, 1464, 2124, 2225, 2339, 2381
Eam1105I GACNNNNNGTC 1 cut(s) 2902
EarI CTCTTC 8 cut(s) 426, 855, 1335, 1464, 2124, 2225, 2339, 2381
EciI GGCGGA 1 cut(s) 649
Eco130I CCWWGG 1 cut(s) 1386
Eco31I GGTCTC 1 cut(s) 917
Eco32I GATATC 1 cut(s) 1357
Eco47I GGWCC 6 cut(s) 719, 799, 1273, 2599, 2691, 2896
Eco57I CTGAAG 9 cut(s) 1200, 1251, 1389, 1434, 1515, 1584, 1974, 2148, 2697
Eco81I CCTNAGG 2 cut(s) 855, 2817
Eco88I CYCGRG 2 cut(s) 2034, 2912
EcoO109I RGGNCCY 4 cut(s) 799, 2599, 2691, 2896
EcoRI GAATTC 1 cut(s) 145
EcoRII CCWGG 5 cut(s) 85, 355, 721, 827, 1275
EcoRV GATATC 1 cut(s) 1357
EcoT14I CCWWGG 1 cut(s) 1386
ErhI CCWWGG 1 cut(s) 1386
Esp3I CGTCTC 1 cut(s) 2518
FalI AAGNNNNNCTT 2 cut(s) 2160, 2192
FaqI GGGAC 2 cut(s) 1889, 2612
FauI CCCGC 2 cut(s) 1865, 2449
FblI GTMKAC 1 cut(s) 1445
FokI GGATG 6 cut(s) 575, 1202, 1253, 1403, 1505, 2572
GsuI CTGGAG 2 cut(s) 2571, 2613
HaeIII GGCC 6 cut(s) 360, 522, 738, 942, 1586, 2810
HapII CCGG 1 cut(s) 2189
HgaI GACGC 2 cut(s) 52, 2714
Hin1I GRCGYC 1 cut(s) 44
HincII GTYRAC 5 cut(s) 37, 1446, 2140, 2215, 2734
HindII GTYRAC 5 cut(s) 37, 1446, 2140, 2215, 2734
HpaI GTTAAC 1 cut(s) 2140
HpaII CCGG 1 cut(s) 2189
Hpy99I CGWCG 1 cut(s) 1447
HpyCH4III ACNGT 8 cut(s) 814, 1249, 2015, 2103, 2488, 2506, 3037, 3109
HpyCH4IV ACGT 5 cut(s) 555, 658, 912, 1442, 2539
HpyF3I CTNAG 6 cut(s) 855, 1632, 1653, 1901, 2406, 2817
HpySE526I ACGT 5 cut(s) 555, 658, 912, 1442, 2539
Hsp92I GRCGYC 1 cut(s) 44
KspAI GTTAAC 1 cut(s) 2140
KspI CCGCGG 1 cut(s) 2445
Kzo9I GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
LguI GCTCTTC 2 cut(s) 426, 2124
LweI GCATC 6 cut(s) 221, 307, 402, 682, 2151, 3106
MabI ACCWGGT 1 cut(s) 721
MaeII ACGT 5 cut(s) 555, 658, 912, 1442, 2539
MaeIII GTNAC 7 cut(s) 551, 601, 659, 985, 1205, 1688, 2039
MalI GATC 6 cut(s) 250, 472, 638, 2175, 2790, 2948
MboI GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
MfeI CAATTG 4 cut(s) 115, 539, 1299, 1407
MflI RGATCY 4 cut(s) 248, 636, 2173, 2946
MhlI GDGCHC 4 cut(s) 812, 1264, 1708, 2442
MlsI TGGCCA 1 cut(s) 942
MluNI TGGCCA 1 cut(s) 942
MlyI GAGTC 6 cut(s) 8, 296, 1022, 1175, 2398, 3104
MmeI TCCRAC 4 cut(s) 547, 1005, 1167, 1484
Mox20I TGGCCA 1 cut(s) 942
MroXI GAANNNNTTC 4 cut(s) 1014, 1966, 2121, 2844
MscI TGGCCA 1 cut(s) 942
MseI TTAA 7 cut(s) 122, 558, 1779, 1884, 2139, 2529, 2994
MslI CAYNNNNRTG 2 cut(s) 2078, 2339
Msp20I TGGCCA 1 cut(s) 942
MspA1I CMGCKG 4 cut(s) 1235, 1813, 2444, 2675
MspI CCGG 1 cut(s) 2189
MspR9I CCNGG 5 cut(s) 87, 357, 723, 829, 1277
MunI CAATTG 4 cut(s) 115, 539, 1299, 1407
MvaI CCWGG 5 cut(s) 87, 357, 723, 829, 1277
MvnI CGCG 1 cut(s) 2444
NcoI CCATGG 1 cut(s) 1386
NdeII GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
NlaIV GGNNCC 5 cut(s) 891, 1365, 2499, 2600, 2692
NmuCI GTSAC 5 cut(s) 601, 659, 1205, 1688, 2039
NspI RCATGY 3 cut(s) 161, 533, 2091
PaeI GCATGC 1 cut(s) 2091
PagI TCATGA 2 cut(s) 1978, 2701
PciI ACATGT 1 cut(s) 529
PciSI GCTCTTC 2 cut(s) 426, 2124
PdmI GAANNNNTTC 4 cut(s) 1014, 1966, 2121, 2844
PfeI GAWTC 5 cut(s) 255, 371, 870, 1636, 1733
PflFI GACNNNGTC 1 cut(s) 2300
PflMI CCANNNNNTGG 1 cut(s) 629
PfoI TCCNGGA 1 cut(s) 85
PleI GAGTC 6 cut(s) 8, 296, 1022, 1174, 2397, 3103
PpsI GAGTC 6 cut(s) 8, 296, 1022, 1174, 2397, 3103
Ppu21I YACGTR 1 cut(s) 2540
PpuMI RGGWCCY 4 cut(s) 799, 2599, 2691, 2896
PscI ACATGT 1 cut(s) 529
Psp5II RGGWCCY 4 cut(s) 799, 2599, 2691, 2896
Psp6I CCWGG 5 cut(s) 85, 355, 721, 827, 1275
PspGI CCWGG 5 cut(s) 85, 355, 721, 827, 1275
PspN4I GGNNCC 5 cut(s) 891, 1365, 2499, 2600, 2692
PspPI GGNCC 8 cut(s) 359, 719, 737, 799, 1273, 2599, 2691, 2896
PspPPI RGGWCCY 4 cut(s) 799, 2599, 2691, 2896
PsrI GAACNNNNNNTAC 2 cut(s) 82, 114
PstI CTGCAG 2 cut(s) 2428, 2470
PstNI CAGNNNCTG 5 cut(s) 278, 629, 1832, 1868, 3125
PsuI RGATCY 4 cut(s) 248, 636, 2173, 2946
PsyI GACNNNGTC 1 cut(s) 2300
PvuII CAGCTG 2 cut(s) 1813, 2675
RsaI GTAC 7 cut(s) 221, 970, 1297, 1378, 2100, 2105, 2621
RsaNI GTAC 7 cut(s) 220, 969, 1296, 1377, 2099, 2104, 2620
RseI CAYNNNNRTG 2 cut(s) 2078, 2339
SacII CCGCGG 1 cut(s) 2445
SalI GTCGAC 1 cut(s) 1444
SapI GCTCTTC 2 cut(s) 426, 2124
SaqAI TTAA 7 cut(s) 122, 558, 1779, 1884, 2139, 2529, 2994
Sau3AI GATC 6 cut(s) 248, 470, 636, 2173, 2788, 2946
Sau96I GGNCC 8 cut(s) 359, 719, 737, 799, 1273, 2599, 2691, 2896
SchI GAGTC 6 cut(s) 8, 296, 1022, 1175, 2398, 3104
ScrFI CCNGG 5 cut(s) 87, 357, 723, 829, 1277
SduI GDGCHC 4 cut(s) 812, 1264, 1708, 2442
SexAI ACCWGGT 1 cut(s) 721
SfaNI GCATC 6 cut(s) 221, 307, 402, 682, 2151, 3106
SfcI CTRYAG 4 cut(s) 2112, 2424, 2466, 2505
Sfr303I CCGCGG 1 cut(s) 2445
SgrBI CCGCGG 1 cut(s) 2445
SinI GGWCC 6 cut(s) 719, 799, 1273, 2599, 2691, 2896
SmiMI CAYNNNNRTG 2 cut(s) 2078, 2339
SmlI CTYRAG 7 cut(s) 366, 1485, 2094, 2237, 2255, 2603, 2879
SmoI CTYRAG 7 cut(s) 366, 1485, 2094, 2237, 2255, 2603, 2879
SphI GCATGC 1 cut(s) 2091
SsiI CCGC 9 cut(s) 634, 1235, 1490, 1872, 2356, 2442, 2444, 2520, 2626
StyD4I CCNGG 5 cut(s) 85, 355, 721, 827, 1275
StyI CCWWGG 1 cut(s) 1386
TaaI ACNGT 8 cut(s) 814, 1249, 2015, 2103, 2488, 2506, 3037, 3109
TaiI ACGT 5 cut(s) 558, 661, 915, 1445, 2542
TaqI TCGA 4 cut(s) 194, 1445, 1741, 2838
TatI WGTACW 3 cut(s) 1295, 1376, 2098
TauI GCSGC 1 cut(s) 2522
TfiI GAWTC 5 cut(s) 255, 371, 870, 1636, 1733
Tru1I TTAA 7 cut(s) 122, 558, 1779, 1884, 2139, 2529, 2994
Tru9I TTAA 7 cut(s) 122, 558, 1779, 1884, 2139, 2529, 2994
TscAI CASTG 7 cut(s) 1063, 1168, 1210, 1729, 1954, 2442, 2475
TseFI GTSAC 5 cut(s) 601, 659, 1205, 1688, 2039
Tsp45I GTSAC 5 cut(s) 601, 659, 1205, 1688, 2039
TspGWI ACGGA 4 cut(s) 45, 187, 947, 1907
TspRI CASTG 7 cut(s) 1063, 1168, 1210, 1729, 1954, 2442, 2475
Tth111I GACNNNGTC 1 cut(s) 2300
Van91I CCANNNNNTGG 1 cut(s) 629
VpaK11BI GGWCC 6 cut(s) 719, 799, 1273, 2599, 2691, 2896
XapI RAATTY 5 cut(s) 128, 145, 820, 1542, 2767
XbaI TCTAGA 2 cut(s) 251, 1309
XceI RCATGY 3 cut(s) 161, 533, 2091
XcmI CCANNNNNNNNNTGG 1 cut(s) 1393
XmiI GTMKAC 1 cut(s) 1445
XmnI GAANNNNTTC 4 cut(s) 1014, 1966, 2121, 2844
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.