FvH4_1g09571

pectinesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015779
Physical Location & Seq
Reverse (-)
5135395 .. 5137867
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09571.t1

Sequence Viewer

Length: 1782 bp
ATGGCATTCCAGGATTTCGACCAGATAACTGAGCGGCGAAAGACCGAGAATGCCCGTAAGTTCAAGAAGAGGATTCTCATCGCAGTTGTTGCGGTGATCCTCCTTATTGGAATTATCGTTGGAGCCATATTCATTGTGAACAAATTGAAGACGCACGAGGCAACCAAAAAAGATAGCAAACCTGCTTCACCACCACCAAAGAGACCAGATTCGGATAATAATTCAACCGATGATAAGCCATCAACTTCTAGCAAGCTTGTAGAGCAGATGTGCGGGTCAACTGATTACAAGGACAAGTGCAAGGAAGTGTTCTCAAAGAAAAAGGACCCCATCTCTAAACCCAGAGAAGTCATCAAGGCCATCATCTCTGGAGCCTCCGATGAGGCCAAGAGTGCCTTCAGCAAAGCCAGCGAGGTCAGTTTTGACAAAGAGGAAGAGAAGAAAGCCTTTGAGGATTGCAAGACACTCTTTGACGATGCCAAGGAAGAACTAGAACAATCCGTGTATCAAGTTGGCAACACTACCAGTTCTGGGAAAATGAGGATCGGTCTCTTGAACAGTTGGTTGAGTGCGGTCATATCATACGGGCAAACATGCATTGATGGGTTTCCTGATGGAGATGGGAAGTCTAAAATTACCAAGACCTTGGAGGCCACCAAGGAGCTCACTAGCAATTCCCTTGCCATGATTTCACTACTTTCCGAAGTACCTGGCAGTAAATCATCATCAGGTTCATCACGTCGTCTTCTGGCACAGGACAAGAATGGCTTTCCTTCCTGGCTGACATATGACGAGCGAAGGGTGTTGAAGAAAAACGATGAAATGCCCACACCCAATGTCACAGTGGCGAAAGATGGCAGTGGGAACTACCAATCCATTAGTCAAGCATTGTCAGCCATGCCTGAAAAATACGAAGGAAGGTATATCATCTATGTTAAAGAAGGAGTCTATAATGAGAGTGTTACTGTGACAAAGAAGATGCCAAATGTTACCATATATGGTGATGGATCACAGAAGAGCATCATCACTGGGAATAAGAACTCGGCAGACGGAGTTAAAATGTTCCAAACTGCAAGTTTTGTGGCCTTAGGAGAAGGTCTCATTGCAAAGTCCATGGGATTCAGAAACACAGCTGGTCGTGACAAGCAACAGGCAGTGGCAGCTAGAGTCCTAGCAGACCGTGCTGTATTCCTCAATTGCCGATTTGAAGGGTACCAAAGCACATTGTTTGTACAGGCTCATCGGCAGTTCTACAAAAGCTCGGTTGTTTCTGGCACCATTGATTTTATCTTTGGTGATGCTGCTGCTATCTTCCAGAACTGCTTAATTTATATCCGCAAACCTCAGGAAAACCATTCGAACACAGTTACAGCCCAAGGAAGAACAGACAAACGTGAAACCACGGGAATAGTGTTGCACAAATGCCAGATTCTACCAGAAAAAGAATTTGAGTCTGAAAAATCCCAATTCAAGAGTTACCTTGGGAGGCCATGGAAGGAGTACTCAAGAACCATAGTGATGGAGTCTACAATAGAGGATGTGATTGACCAACAGGGATGGTATGAAATGGATGGAGACTTTGGAAAAAAGACTCTATTTTATGGTGAGTATAACAACAATGGAGACAGTTCCAAGACTGATAACAGAGTAACATGGCCTGGCTTCAAGGTCCTTAACAAGGATGAGGCTATGCAATTTACTGTAGGTCCCTTCTTGAAAGGGAATGCTTGGCTCAAGAATAACAATGGTGTTCCTGTTCGATTTGGCTTGTTTGATAACTAG

Protein Analysis

594

Amino Acids

66.05

Weight (kDa)

8.96

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PMEI PF04043 85 - 229 3.5e-29 Plant invertase/pectin methylesterase inhibitor
Pectinesterase PF01095 279 - 575 6.7e-127 Pectinesterase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes (Group: OG0015779)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G26450 AT4G33230
fragaria_vesca FvH4_1g09571
malus_domestica MD02G1104300.v1.1 MD15G1224600.v1.1
prunus_persica Prupe.7G190500_v2.0.a1
rosa_laevigata RLG00000016639
rosa_multiflora Rmu_sc0004244.1_g000003
rosa_roxburghii Rroxscaffold_2G00145660
rosa_rugosa Rorug02G0057900
rosa_samantha Rh2AG104600 Rh2BG106800 Rh2CG108600 Rh2DG107700
rosa_wichuraiana Rw2G008080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 190
Acc65I GGTACC 1 cut(s) 1212
AccB1I GGYRCC 2 cut(s) 1212, 1274
AccBSI CCGCTC 1 cut(s) 34
AccI GTMKAC 1 cut(s) 1526
AciI CCGC 5 cut(s) 34, 92, 273, 572, 1336
AclWI GGATC 3 cut(s) 91, 551, 1015
AcsI RAATTY 1 cut(s) 1445
AcuI CTGAAG 1 cut(s) 382
AfaI GTAC 4 cut(s) 708, 1214, 1233, 1502
AfiI CCNNNNNNNGG 2 cut(s) 531, 1678
AgsI TTSAA 9 cut(s) 64, 148, 225, 556, 808, 1208, 1471, 1666, 1717
AjiI CACGTC 1 cut(s) 740
AjnI CCWGG 4 cut(s) 9, 709, 776, 1657
AjuI GAANNNNNNNTTGG 2 cut(s) 380, 412
AloI GAACNNNNNNTCC 2 cut(s) 857, 889
AluBI AGCT 5 cut(s) 256, 664, 1133, 1163, 1260
AluI AGCT 5 cut(s) 256, 664, 1133, 1163, 1260
Alw21I GWGCWC 1 cut(s) 666
Alw26I GTCTC 5 cut(s) 196, 554, 1103, 1569, 1617
AlwI GGATC 3 cut(s) 91, 551, 1015
AoxI GGCC 6 cut(s) 357, 384, 651, 1083, 1487, 1655
ApeKI GCWGC 3 cut(s) 1160, 1301, 1304
ApoI RAATTY 1 cut(s) 1445
Asp718I GGTACC 1 cut(s) 1212
AspS9I GGNCC 3 cut(s) 325, 1669, 1706
AsuHPI GGTGA 5 cut(s) 106, 180, 1013, 1307, 1616
AsuII TTCGAA 1 cut(s) 1358
AvaII GGWCC 3 cut(s) 325, 1669, 1706
AxyI CCTNAGG 2 cut(s) 1087, 1344
BanI GGYRCC 2 cut(s) 1212, 1274
BanII GRGCYC 1 cut(s) 666
BauI CACGAG 1 cut(s) 155
BbsI GAAGAC 2 cut(s) 155, 737
Bbv12I GWGCWC 1 cut(s) 666
BbvI GCAGC 3 cut(s) 1172, 1288, 1291
BciT130I CCWGG 4 cut(s) 11, 711, 778, 1659
BcoDI GTCTC 5 cut(s) 196, 554, 1103, 1569, 1617
BfaI CTAG 6 cut(s) 249, 491, 669, 1164, 1172, 1780
BfmI CTRYAG 1 cut(s) 1701
BfuAI ACCTGC 1 cut(s) 190
BisI GCNGC 4 cut(s) 35, 1161, 1302, 1305
BlsI GCNGC 4 cut(s) 36, 1162, 1303, 1306
BmcAI AGTACT 1 cut(s) 1502
Bme1390I CCNGG 4 cut(s) 11, 711, 778, 1659
Bme18I GGWCC 3 cut(s) 325, 1669, 1706
BmgBI CACGTC 1 cut(s) 740
BmgT120I GGNCC 3 cut(s) 325, 1669, 1706
BmiI GGNNCC 6 cut(s) 124, 327, 373, 1214, 1276, 1708
BmrFI CCNGG 4 cut(s) 11, 711, 778, 1659
BmrI ACTGGG 1 cut(s) 1038
BmsI GCATC 4 cut(s) 466, 969, 1029, 1288
BmuI ACTGGG 1 cut(s) 1038
BpiI GAAGAC 2 cut(s) 155, 737
BplI GAGNNNNNCTC 2 cut(s) 1083, 1115
BpmI CTGGAG 1 cut(s) 390
Bpu14I TTCGAA 1 cut(s) 1358
BpuEI CTTGAG 2 cut(s) 1489, 1718
BsaBI GATNNNNATC 1 cut(s) 77
BsaI GGTCTC 3 cut(s) 196, 554, 1103
BsaJI CCNNGG 8 cut(s) 480, 645, 657, 1113, 1375, 1401, 1480, 1490
Bsc4I CCNNNNNNNGG 2 cut(s) 531, 1678
Bse1I ACTGG 2 cut(s) 525, 1033
Bse21I CCTNAGG 2 cut(s) 1087, 1344
Bse3DI GCAATG 1 cut(s) 1101
Bse8I GATNNNNATC 1 cut(s) 77
BseBI CCWGG 4 cut(s) 11, 711, 778, 1659
BseDI CCNNGG 8 cut(s) 480, 645, 657, 1113, 1375, 1401, 1480, 1490
BseGI GGATG 4 cut(s) 1543, 1562, 1576, 1687
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 2 cut(s) 531, 1678
BseMI GCAATG 1 cut(s) 1101
BseMII CTCAG 2 cut(s) 21, 1358
BseNI ACTGG 2 cut(s) 525, 1033
BseXI GCAGC 3 cut(s) 1172, 1288, 1291
BshFI GGCC 6 cut(s) 359, 386, 653, 1085, 1489, 1657
BshNI GGYRCC 2 cut(s) 1212, 1274
BsiHKAI GWGCWC 1 cut(s) 666
BslFI GGGAC 1 cut(s) 1692
BslI CCNNNNNNNGG 2 cut(s) 531, 1678
BsmAI GTCTC 5 cut(s) 196, 554, 1103, 1569, 1617
BsmFI GGGAC 1 cut(s) 1692
BsmI GAATGC 3 cut(s) 5, 55, 1729
BsnI GGCC 6 cut(s) 359, 386, 653, 1085, 1489, 1657
Bso31I GGTCTC 3 cut(s) 196, 554, 1103
Bsp119I TTCGAA 1 cut(s) 1358
Bsp1286I GDGCHC 1 cut(s) 666
Bsp1407I TGTACA 1 cut(s) 1231
Bsp143I GATC 3 cut(s) 96, 543, 1007
Bsp19I CCATGG 2 cut(s) 1113, 1490
BspACI CCGC 5 cut(s) 34, 92, 273, 572, 1336
BspANI GGCC 6 cut(s) 359, 386, 653, 1085, 1489, 1657
BspCNI CTCAG 2 cut(s) 22, 1357
BspLI GGNNCC 6 cut(s) 124, 327, 373, 1214, 1276, 1708
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 91, 551, 1015
BspQI GCTCTTC 1 cut(s) 1010
BspT104I TTCGAA 1 cut(s) 1358
BspT107I GGYRCC 2 cut(s) 1212, 1274
BspTNI GGTCTC 3 cut(s) 196, 554, 1103
BsrBI CCGCTC 1 cut(s) 34
BsrDI GCAATG 1 cut(s) 1101
BsrGI TGTACA 1 cut(s) 1231
BsrI ACTGG 2 cut(s) 525, 1033
BssECI CCNNGG 8 cut(s) 480, 645, 657, 1113, 1375, 1401, 1480, 1490
BssMI GATC 3 cut(s) 96, 543, 1007
BssSI CACGAG 1 cut(s) 155
BssT1I CCWWGG 7 cut(s) 480, 645, 657, 1113, 1375, 1480, 1490
Bst2BI CACGAG 1 cut(s) 155
Bst2UI CCWGG 4 cut(s) 11, 711, 778, 1659
Bst4CI ACNGT 7 cut(s) 560, 844, 967, 1181, 1366, 1628, 1702
Bst6I CTCTTC 3 cut(s) 62, 429, 1010
BstAPI GCANNNNNTGC 2 cut(s) 89, 1181
BstAUI TGTACA 1 cut(s) 1231
BstBI TTCGAA 1 cut(s) 1358
BstC8I GCNNGC 2 cut(s) 254, 409
BstDEI CTNAG 3 cut(s) 30, 1087, 1344
BstDSI CCRYGG 3 cut(s) 1113, 1401, 1490
BstENI CCTNNNNNAGG 1 cut(s) 1676
BstF5I GGATG 4 cut(s) 1543, 1562, 1576, 1687
BstKTI GATC 3 cut(s) 99, 546, 1010
BstMAI GTCTC 5 cut(s) 196, 554, 1103, 1569, 1617
BstMBI GATC 3 cut(s) 96, 543, 1007
BstMWI GCNNNNNNNGC 7 cut(s) 89, 262, 392, 408, 893, 1160, 1181
BstNI CCWGG 4 cut(s) 11, 711, 778, 1659
BstNSI RCATGY 1 cut(s) 597
BstSCI CCNGG 4 cut(s) 9, 709, 776, 1657
BstSFI CTRYAG 1 cut(s) 1701
BstV1I GCAGC 3 cut(s) 1172, 1288, 1291
BstV2I GAAGAC 2 cut(s) 155, 737
BstXI CCANNNNNNTGG 2 cut(s) 646, 1519
Bsu36I CCTNAGG 2 cut(s) 1087, 1344
BsuRI GGCC 6 cut(s) 359, 386, 653, 1085, 1489, 1657
BtgI CCRYGG 3 cut(s) 1113, 1401, 1490
BtgZI GCGATG 1 cut(s) 64
BtrI CACGTC 1 cut(s) 740
BtsCI GGATG 4 cut(s) 1543, 1562, 1576, 1687
BtsI GCAGTG 2 cut(s) 865, 1161
BtsIMutI CAGTG 4 cut(s) 849, 865, 1026, 1161
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 2 cut(s) 254, 409
Cfr13I GGNCC 3 cut(s) 325, 1669, 1706
CseI GACGC 1 cut(s) 160
Csp6I GTAC 4 cut(s) 707, 1213, 1232, 1501
CspCI CAANNNNNGTGG 2 cut(s) 1062, 1097
CviAII CATG 6 cut(s) 594, 685, 898, 1114, 1491, 1653
CviQI GTAC 4 cut(s) 707, 1213, 1232, 1501
DdeI CTNAG 3 cut(s) 30, 1087, 1344
DpnI GATC 3 cut(s) 98, 545, 1009
DpnII GATC 3 cut(s) 96, 543, 1007
Eam1104I CTCTTC 3 cut(s) 62, 429, 1010
EarI CTCTTC 3 cut(s) 62, 429, 1010
Ecl136II GAGCTC 1 cut(s) 664
Eco130I CCWWGG 7 cut(s) 480, 645, 657, 1113, 1375, 1480, 1490
Eco24I GRGCYC 1 cut(s) 666
Eco31I GGTCTC 3 cut(s) 196, 554, 1103
Eco47I GGWCC 3 cut(s) 325, 1669, 1706
Eco53kI GAGCTC 1 cut(s) 664
Eco57I CTGAAG 1 cut(s) 382
Eco81I CCTNAGG 2 cut(s) 1087, 1344
EcoICRI GAGCTC 1 cut(s) 664
EcoNI CCTNNNNNAGG 1 cut(s) 1676
EcoO109I RGGNCCY 3 cut(s) 325, 1669, 1706
EcoRII CCWGG 4 cut(s) 9, 709, 776, 1657
EcoT14I CCWWGG 7 cut(s) 480, 645, 657, 1113, 1375, 1480, 1490
EcoT22I ATGCAT 1 cut(s) 599
EcoT38I GRGCYC 1 cut(s) 666
ErhI CCWWGG 7 cut(s) 480, 645, 657, 1113, 1375, 1480, 1490
FaeI CATG 6 cut(s) 597, 688, 901, 1117, 1494, 1656
FalI AAGNNNNNCTT 8 cut(s) 380, 412, 431, 463, 452, 484, 752, 784
FaqI GGGAC 1 cut(s) 1692
FatI CATG 6 cut(s) 593, 684, 897, 1113, 1490, 1652
FauI CCCGC 1 cut(s) 266
FauNDI CATATG 1 cut(s) 787
FblI GTMKAC 1 cut(s) 1526
Fnu4HI GCNGC 4 cut(s) 35, 1161, 1302, 1305
FokI GGATG 4 cut(s) 1550, 1569, 1583, 1694
FriOI GRGCYC 1 cut(s) 666
Fsp4HI GCNGC 4 cut(s) 35, 1161, 1302, 1305
FspBI CTAG 6 cut(s) 249, 491, 669, 1164, 1172, 1780
GluI GCNGC 4 cut(s) 35, 1161, 1302, 1305
GsuI CTGGAG 1 cut(s) 390
HaeIII GGCC 6 cut(s) 359, 386, 653, 1085, 1489, 1657
HgaI GACGC 1 cut(s) 160
Hin1II CATG 6 cut(s) 597, 688, 901, 1117, 1494, 1656
HincII GTYRAC 1 cut(s) 279
HindII GTYRAC 1 cut(s) 279
HindIII AAGCTT 1 cut(s) 254
HinfI GANTC 9 cut(s) 73, 209, 945, 1119, 1167, 1429, 1451, 1523, 1591
HphI GGTGA 5 cut(s) 106, 180, 1013, 1307, 1616
Hpy166II GTNNAC 3 cut(s) 139, 279, 1527
Hpy188I TCNGA 5 cut(s) 214, 379, 703, 1124, 1456
Hpy8I GTNNAC 3 cut(s) 139, 279, 1527
Hpy99I CGWCG 1 cut(s) 744
HpyCH4III ACNGT 7 cut(s) 560, 844, 967, 1181, 1366, 1628, 1702
HpyCH4IV ACGT 2 cut(s) 739, 1393
HpyCH4V TGCA 7 cut(s) 300, 459, 597, 1073, 1106, 1417, 1693
HpyF10VI GCNNNNNNNGC 7 cut(s) 89, 262, 392, 408, 893, 1160, 1181
HpyF3I CTNAG 3 cut(s) 30, 1087, 1344
HpySE526I ACGT 2 cut(s) 739, 1393
Hsp92II CATG 6 cut(s) 597, 688, 901, 1117, 1494, 1656
KpnI GGTACC 1 cut(s) 1216
Kzo9I GATC 3 cut(s) 96, 543, 1007
LguI GCTCTTC 1 cut(s) 1010
LmnI GCTCC 3 cut(s) 122, 371, 661
Lsp1109I GCAGC 3 cut(s) 1172, 1288, 1291
LweI GCATC 4 cut(s) 466, 969, 1029, 1288
MaeI CTAG 6 cut(s) 249, 491, 669, 1164, 1172, 1780
MaeII ACGT 2 cut(s) 739, 1393
MaeIII GTNAC 8 cut(s) 838, 961, 967, 988, 1139, 1366, 1475, 1648
MalI GATC 3 cut(s) 98, 545, 1009
MbiI CCGCTC 1 cut(s) 34
MboI GATC 3 cut(s) 96, 543, 1007
MfeI CAATTG 1 cut(s) 1195
MhlI GDGCHC 1 cut(s) 666
MlyI GAGTC 5 cut(s) 954, 1176, 1460, 1532, 1585
MmeI TCCRAC 1 cut(s) 100
Mph1103I ATGCAT 1 cut(s) 599
MseI TTAA 4 cut(s) 936, 1056, 1325, 1674
MslI CAYNNNNRTG 1 cut(s) 1517
MspA1I CMGCKG 1 cut(s) 1133
MspR9I CCNGG 4 cut(s) 11, 711, 778, 1659
MunI CAATTG 1 cut(s) 1195
Mva1269I GAATGC 3 cut(s) 5, 55, 1729
MvaI CCWGG 4 cut(s) 11, 711, 778, 1659
MwoI GCNNNNNNNGC 7 cut(s) 89, 262, 392, 408, 893, 1160, 1181
NcoI CCATGG 2 cut(s) 1113, 1490
NdeI CATATG 1 cut(s) 787
NdeII GATC 3 cut(s) 96, 543, 1007
NlaIII CATG 6 cut(s) 597, 688, 901, 1117, 1494, 1656
NlaIV GGNNCC 6 cut(s) 124, 327, 373, 1214, 1276, 1708
NmeAIII GCCGAG 1 cut(s) 1022
NmuCI GTSAC 3 cut(s) 838, 967, 1139
NsiI ATGCAT 1 cut(s) 599
NspI RCATGY 1 cut(s) 597
NspV TTCGAA 1 cut(s) 1358
PciSI GCTCTTC 1 cut(s) 1010
PctI GAATGC 3 cut(s) 5, 55, 1729
PfeI GAWTC 4 cut(s) 73, 209, 1119, 1429
PfoI TCCNGGA 1 cut(s) 9
PkrI GCNGC 4 cut(s) 36, 1162, 1303, 1306
PleI GAGTC 5 cut(s) 953, 1175, 1459, 1531, 1585
PpsI GAGTC 5 cut(s) 953, 1175, 1459, 1531, 1585
PpuMI RGGWCCY 3 cut(s) 325, 1669, 1706
Psp124BI GAGCTC 1 cut(s) 666
Psp5II RGGWCCY 3 cut(s) 325, 1669, 1706
Psp6I CCWGG 4 cut(s) 9, 709, 776, 1657
PspGI CCWGG 4 cut(s) 9, 709, 776, 1657
PspN4I GGNNCC 6 cut(s) 124, 327, 373, 1214, 1276, 1708
PspPI GGNCC 3 cut(s) 325, 1669, 1706
PspPPI RGGWCCY 3 cut(s) 325, 1669, 1706
PvuII CAGCTG 1 cut(s) 1133
RsaI GTAC 4 cut(s) 708, 1214, 1233, 1502
RsaNI GTAC 4 cut(s) 707, 1213, 1232, 1501
RseI CAYNNNNRTG 1 cut(s) 1517
SacI GAGCTC 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 1010
SaqAI TTAA 4 cut(s) 936, 1056, 1325, 1674
SatI GCNGC 4 cut(s) 35, 1161, 1302, 1305
Sau3AI GATC 3 cut(s) 96, 543, 1007
Sau96I GGNCC 3 cut(s) 325, 1669, 1706
ScaI AGTACT 1 cut(s) 1502
SchI GAGTC 5 cut(s) 954, 1176, 1460, 1532, 1585
ScrFI CCNGG 4 cut(s) 11, 711, 778, 1659
SduI GDGCHC 1 cut(s) 666
SfaNI GCATC 4 cut(s) 466, 969, 1029, 1288
SfcI CTRYAG 1 cut(s) 1701
SfuI TTCGAA 1 cut(s) 1358
SinI GGWCC 3 cut(s) 325, 1669, 1706
SmiMI CAYNNNNRTG 1 cut(s) 1517
SmlI CTYRAG 2 cut(s) 1504, 1733
SmoI CTYRAG 2 cut(s) 1504, 1733
SsiI CCGC 5 cut(s) 34, 92, 273, 572, 1336
SspMI CTAG 6 cut(s) 249, 491, 669, 1164, 1172, 1780
SstI GAGCTC 1 cut(s) 666
StyD4I CCNGG 4 cut(s) 9, 709, 776, 1657
StyI CCWWGG 7 cut(s) 480, 645, 657, 1113, 1375, 1480, 1490
TaaI ACNGT 7 cut(s) 560, 844, 967, 1181, 1366, 1628, 1702
TaiI ACGT 2 cut(s) 742, 1396
TaqI TCGA 3 cut(s) 18, 1358, 1759
TaqII GACCGA 2 cut(s) 59, 536
TatI WGTACW 2 cut(s) 1231, 1500
TauI GCSGC 1 cut(s) 37
TfiI GAWTC 4 cut(s) 73, 209, 1119, 1429
Tru1I TTAA 4 cut(s) 936, 1056, 1325, 1674
Tru9I TTAA 4 cut(s) 936, 1056, 1325, 1674
TscAI CASTG 4 cut(s) 849, 865, 1033, 1161
TseFI GTSAC 3 cut(s) 838, 967, 1139
TseI GCWGC 3 cut(s) 1160, 1301, 1304
Tsp45I GTSAC 3 cut(s) 838, 967, 1139
TspDTI ATGAA 4 cut(s) 121, 723, 834, 1578
TspGWI ACGGA 2 cut(s) 490, 1065
TspRI CASTG 4 cut(s) 849, 865, 1033, 1161
VpaK11BI GGWCC 3 cut(s) 325, 1669, 1706
XagI CCTNNNNNAGG 1 cut(s) 1676
XapI RAATTY 1 cut(s) 1445
XceI RCATGY 1 cut(s) 597
XcmI CCANNNNNNNNNTGG 1 cut(s) 841
XmiI GTMKAC 1 cut(s) 1526
XspI CTAG 6 cut(s) 249, 491, 669, 1164, 1172, 1780
ZrmI AGTACT 1 cut(s) 1502
Zsp2I ATGCAT 1 cut(s) 599
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.