Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr15

Orthogroup ID

OG0015779

Physical Location & Seq

Genomic Coordinates Reverse (-)

18269437 .. 18271831

Gene Structure 2395 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD15G1224600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1812 bp

ATGGCATTTCAGGATTTCGACCAGCTTACCGAACGCCGAAAGACCGAGAATGCACGTAAGTTCAAGAAGAGAATTATCATTGCGGTCGTCGTCATTCTCCTCCTTGTTCTGATTGCCGTCGGAGCCTATTTCATTGTCAACAAACTTAATAATAAAGGTACTAAGAAGGGCAATGCAAAGGAGGGCAATGCTAAACAAGGCAAAGCCGCAGGGAAACCTGCACCGAAGGCTCCTCAACCGAAAAATGGACCACCGAAGAGCAAAACGCCTTCGAATGGTCAGAAGATCCTGAAGGAGATGTGCAGCGCTGCAGATTACAAGGAAAAATGCGAGGGCATCATTGAAAAGGCAAGGGACGAAACAAAACCCAAGGCATTCATCAAGACCGCTATCTCAGCCGCCTCGGACGAGGCCAAGAGTGCCTACAGCAAAACTGCCGAGCTGACTTTCAACAGTCCGGAAGAGAAGGGAGCGTTTGAGGATTGCAAAGTGTTGTTCGACGATGCCAAGGGGGAATTAGGGGACGCCGTTTCTCAGCTTGGCAACAGCATTGAGTCGGGGAAGGTTCGCACAGGCGTCTTGAACAGCTGGTTGAGTGCGGTGATATCTTACCAGCAGACATGCGTCGACGGGTTTCCTGATGGAAAATTGAAGTCCGACTTGGAGAAGATGTTGCAGGCCTCTAAGGAATTCACTAGCAACTCCCTGGCCATGCTTTCACTCTTTAACCAGTTGCAGCTACCGGGTGCAGAAGCAGCTACAGGATCCAATCGCCGCCTTCTATCGCAGGACAAGAGCGGATTCCCTACCTGGATGAGTCACGAGGACCGAAGGATGTTGAAGAAAAACGATGAAAAGCTCACCCCCAACGTGACGGTGGCAAAAGATGGTAGTGGAAACTACAAAACCATTAGTGAAGCCTTGGAAAAAATACCCGAAAAATACGAAGGCCGGTATGTCATCTATGTTAAAGAAGGAGTTTATGATGAGACCGTGACTGTGACAAAGAAGATGCCGAACGTTACAATGTACGGCGATGGGTCACAGAAGAGCGTCGTCACTGGAAATAAAAACTTTGCCGATAAAGTTAGGATATTCCAAACTGCACCTTTCGCGGTCTTAGGGGAAGGTTTTATGGCAAAGTCGATGGGATTCAGAAACACGGCTGGACCTGAGAAGCATCAGGCAGTGGCAGCAAGAGTCCAAGCAGACCGCGCTATTTTTCTGAACTGCCGGTTTGAAGGGTACCAAAGCACATTATACGCACAAACTCATCGGCAGTTCTATAAGAGCTGTGTCATTTCAGGCACCGTTGATTTCATCTTTGGCGACGCTGCTGCCATCTTCCAGAACTGCTTGATCTACGTCCGCAAGCCTTTGGAAAACCAACAAAACACTGTCACAGCCCAGGGAAGGACTGACAAACAGGAAACCACAGGAATAGTGTTGCAGAATTGCAAAATCATGCCGGACAAAGATCTTGAGCCAGTAAAGTCCCAATTCAAGACCTACCTCGGAAGGCCATCGAAGGAATTCTCGAGAACCATAGTGATGGATTCGACAATTGAGGACCTGATTCACCCCGATGGATGGACACAGTGGGAAGGAGATTTCGCACTAAAAACATTGTATTATGCAGAATATAACAACAAGGGACCGGGCGCGAAAACTGACAACAGAGTGAAGTGGGATGGATACAAGAAAATTGACAAGCAAGAGGCAATGCATTATACTGTAGGGCCTTTCTTGAAGGGGCACGCTTGGCTTATGGGTGTGGGTGTTCCTGCTCGCTTCGGCCTATTTGAAAGTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004857 GO:0005575 GO:0005623 GO:0008150 GO:0016787 GO:0016788 GO:0030234 GO:0030599 GO:0043086 GO:0044092 GO:0044464 GO:0046910 GO:0050790 GO:0052689 GO:0065007 GO:0065009 GO:0071944 GO:0098772

KEGG Pathways

Metabolic & Signaling

ko00040 ko01100 map00040 map01100

Pfam Domains

Protein Families

PMEI Pectinesterase

Protein Analysis

604

Amino Acids

66.9

Weight (kDa)

9.25

Isoelectric Point (pI)

31.55

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PMEI	PF04043	96 - 238	1.6e-27	Plant invertase/pectin methylesterase inhibitor
Pectinesterase	PF01095	290 - 585	1.8e-139	Pectinesterase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0015779)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G26450 AT4G33230
fragaria_vesca	FvH4_1g09571
malus_domestica	MD02G1104300.v1.1 MD15G1224600.v1.1
prunus_persica	Prupe.7G190500_v2.0.a1
rosa_laevigata	RLG00000016639
rosa_multiflora	Rmu_sc0004244.1_g000003
rosa_roxburghii	Rroxscaffold_2G00145660
rosa_rugosa	Rorug02G0057900
rosa_samantha	Rh2AG104600 Rh2BG106800 Rh2CG108600 Rh2DG107700
rosa_wichuraiana	Rw2G008080

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	226
Acc65I	GGTACC	1 cut(s)	1245
AccB1I	GGYRCC	2 cut(s)	1245, 1307
AccBSI	CCGCTC	1 cut(s)	798
AccI	GTMKAC	1 cut(s)	627
AccII	CGCG	3 cut(s)	1115, 1215, 1664
AccIII	TCCGGA	1 cut(s)	457
AclI	AACGTT	1 cut(s)	1020
AclWI	GGATC	3 cut(s)	280, 759, 772
AcoI	YGGCCR	1 cut(s)	708
AcsI	RAATTY	2 cut(s)	689, 1532
AcuI	CTGAAG	1 cut(s)	311
AcyI	GRCGYC	2 cut(s)	525, 576
AfaI	GTAC	3 cut(s)	160, 1031, 1247
AfeI	AGCGCT	1 cut(s)	307
AfiI	CCNNNNNNNGG	4 cut(s)	245, 275, 1413, 1590
AjnI	CCWGG	3 cut(s)	705, 809, 1407
AjuI	GAANNNNNNNTTGG	2 cut(s)	644, 676
AluBI	AGCT	8 cut(s)	25, 442, 538, 588, 739, 758, 859, 1293
AluI	AGCT	8 cut(s)	25, 442, 538, 588, 739, 758, 859, 1293
Alw26I	GTCTC	1 cut(s)	983
AlwI	GGATC	3 cut(s)	280, 759, 772
Ama87I	CYCGRG	1 cut(s)	1537
Aor13HI	TCCGGA	1 cut(s)	457
Aor51HI	AGCGCT	1 cut(s)	307
AoxI	GGCC	7 cut(s)	411, 678, 708, 949, 1520, 1739, 1795
ApeKI	GCWGC	7 cut(s)	303, 308, 736, 755, 1193, 1334, 1337
ApoI	RAATTY	2 cut(s)	689, 1532
Asp700I	GAANNNNTTC	1 cut(s)	1532
Asp718I	GGTACC	1 cut(s)	1245
AspLEI	GCGC	3 cut(s)	308, 1217, 1664
AspS9I	GGNCC	6 cut(s)	248, 826, 1169, 1570, 1655, 1739
AsuC2I	CCSGG	2 cut(s)	744, 1659
AsuHPI	GGTGA	3 cut(s)	613, 853, 1571
AsuII	TTCGAA	1 cut(s)	272
AvaI	CYCGRG	1 cut(s)	1537
AvaII	GGWCC	5 cut(s)	248, 826, 1169, 1570, 1655
BaeGI	GKGCMC	1 cut(s)	1758
BalI	TGGCCA	1 cut(s)	710
BamHI	GGATCC	1 cut(s)	764
BanI	GGYRCC	2 cut(s)	1245, 1307
BauI	CACGAG	1 cut(s)	821
BbvI	GCAGC	7 cut(s)	295, 315, 748, 767, 1205, 1321, 1324
BceAI	ACGGC	4 cut(s)	101, 512, 1048, 1179
BcgI	CGANNNNNNTGC	2 cut(s)	1319, 1353
BciT130I	CCWGG	3 cut(s)	707, 811, 1409
BciVI	GTATCC	1 cut(s)	1688
BcnI	CCSGG	2 cut(s)	744, 1659
BcoDI	GTCTC	1 cut(s)	983
BfaI	CTAG	1 cut(s)	696
BfmI	CTRYAG	4 cut(s)	309, 424, 759, 1734
BfoI	RGCGCY	1 cut(s)	309
BfuAI	ACCTGC	1 cut(s)	226
BfuI	GTATCC	1 cut(s)	1688
BglII	AGATCT	1 cut(s)	1477
Bme1390I	CCNGG	5 cut(s)	707, 744, 811, 1409, 1659
Bme18I	GGWCC	5 cut(s)	248, 826, 1169, 1570, 1655
BmeT110I	CYCGRG	1 cut(s)	1537
BmgT120I	GGNCC	6 cut(s)	248, 826, 1169, 1570, 1655, 1739
BmiI	GGNNCC	6 cut(s)	124, 231, 766, 1247, 1309, 1656
BmrFI	CCNGG	5 cut(s)	707, 744, 811, 1409, 1659
BmsI	GCATC	4 cut(s)	345, 493, 1002, 1189
BoxI	GACNNNNGTC	1 cut(s)	623
Bpu14I	TTCGAA	1 cut(s)	272
BpuEI	CTTGAG	1 cut(s)	1502
BpuMI	CCSGG	2 cut(s)	744, 1659
BsaAI	YACGTR	1 cut(s)	56
BsaHI	GRCGYC	2 cut(s)	525, 576
BsaI	GGTCTC	1 cut(s)	983
BsaJI	CCNNGG	8 cut(s)	369, 402, 507, 705, 921, 1407, 1408, 1513
BsaWI	WCCGGW	1 cut(s)	457
BsaXI	ACNNNNNCTCC	2 cut(s)	656, 686
Bsc4I	CCNNNNNNNGG	4 cut(s)	245, 275, 1413, 1590
Bse118I	RCCGGY	2 cut(s)	951, 1233
Bse1I	ACTGG	3 cut(s)	730, 1066, 1487
Bse3DI	GCAATG	4 cut(s)	78, 178, 193, 1728
BseAI	TCCGGA	1 cut(s)	457
BseBI	CCWGG	3 cut(s)	707, 811, 1409
BseDI	CCNNGG	8 cut(s)	369, 402, 507, 705, 921, 1407, 1408, 1513
BseGI	GGATG	4 cut(s)	819, 840, 1595, 1696
BseLI	CCNNNNNNNGG	4 cut(s)	245, 275, 1413, 1590
BseMI	GCAATG	4 cut(s)	78, 178, 193, 1728
BseMII	CTCAG	3 cut(s)	408, 548, 1164
BseNI	ACTGG	3 cut(s)	730, 1066, 1487
BseRI	GAGGAG	2 cut(s)	89, 222
BseSI	GKGCMC	1 cut(s)	1758
BseXI	GCAGC	7 cut(s)	295, 315, 748, 767, 1205, 1321, 1324
BsgI	GTGCAG	4 cut(s)	204, 322, 768, 1089
Bsh1236I	CGCG	3 cut(s)	1115, 1215, 1664
Bsh1285I	CGRYCG	1 cut(s)	87
BshFI	GGCC	7 cut(s)	413, 680, 710, 951, 1522, 1741, 1797
BshNI	GGYRCC	2 cut(s)	1245, 1307
BsiEI	CGRYCG	1 cut(s)	87
BsiHKCI	CYCGRG	1 cut(s)	1537
BsiSI	CCGG	6 cut(s)	458, 743, 952, 1234, 1469, 1658
BslFI	GGGAC	4 cut(s)	368, 536, 1480, 1668
BslI	CCNNNNNNNGG	4 cut(s)	245, 275, 1413, 1590
BsmAI	GTCTC	1 cut(s)	983
BsmFI	GGGAC	4 cut(s)	368, 536, 1480, 1668
BsmI	GAATGC	2 cut(s)	55, 374
BsnI	GGCC	7 cut(s)	413, 680, 710, 951, 1522, 1741, 1797
Bso31I	GGTCTC	1 cut(s)	983
BsoBI	CYCGRG	1 cut(s)	1537
Bsp119I	TTCGAA	1 cut(s)	272
Bsp1286I	GDGCHC	1 cut(s)	1758
Bsp13I	TCCGGA	1 cut(s)	457
Bsp143I	GATC	4 cut(s)	285, 764, 1359, 1477
BspANI	GGCC	7 cut(s)	413, 680, 710, 951, 1522, 1741, 1797
BspCNI	CTCAG	3 cut(s)	407, 547, 1165
BspEI	TCCGGA	1 cut(s)	457
BspFNI	CGCG	3 cut(s)	1115, 1215, 1664
BspLI	GGNNCC	6 cut(s)	124, 231, 766, 1247, 1309, 1656
BspMAI	CTGCAG	1 cut(s)	313
BspMI	ACCTGC	1 cut(s)	226
BspPI	GGATC	3 cut(s)	280, 759, 772
BspQI	GCTCTTC	2 cut(s)	251, 1043
BspT104I	TTCGAA	1 cut(s)	272
BspT107I	GGYRCC	2 cut(s)	1245, 1307
BspTNI	GGTCTC	1 cut(s)	983
BsrBI	CCGCTC	1 cut(s)	798
BsrDI	GCAATG	4 cut(s)	78, 178, 193, 1728
BsrFI	RCCGGY	2 cut(s)	951, 1233
BsrI	ACTGG	3 cut(s)	730, 1066, 1487
BssAI	RCCGGY	2 cut(s)	951, 1233
BssECI	CCNNGG	8 cut(s)	369, 402, 507, 705, 921, 1407, 1408, 1513
BssMI	GATC	4 cut(s)	285, 764, 1359, 1477
BssNI	GRCGYC	2 cut(s)	525, 576
BssSI	CACGAG	1 cut(s)	821
BssT1I	CCWWGG	3 cut(s)	369, 507, 921
Bst2BI	CACGAG	1 cut(s)	821
Bst2UI	CCWGG	3 cut(s)	707, 811, 1409
Bst4CI	ACNGT	8 cut(s)	455, 877, 994, 1000, 1312, 1399, 1599, 1735
Bst6I	CTCTTC	4 cut(s)	62, 251, 456, 1043
BstACI	GRCGYC	2 cut(s)	525, 576
BstBAI	YACGTR	1 cut(s)	56
BstBI	TTCGAA	1 cut(s)	272
BstC8I	GCNNGC	4 cut(s)	678, 1373, 1758, 1789
BstDEI	CTNAG	6 cut(s)	162, 394, 534, 684, 1120, 1173
BstF5I	GGATG	4 cut(s)	819, 840, 1595, 1696
BstFNI	CGCG	3 cut(s)	1115, 1215, 1664
BstH2I	RGCGCY	1 cut(s)	309
BstHHI	GCGC	3 cut(s)	308, 1217, 1664
BstKTI	GATC	4 cut(s)	288, 767, 1362, 1480
BstMAI	GTCTC	1 cut(s)	983
BstMBI	GATC	4 cut(s)	285, 764, 1359, 1477
BstMCI	CGRYCG	1 cut(s)	87
BstMWI	GCNNNNNNNGC	9 cut(s)	122, 227, 395, 419, 755, 1112, 1193, 1214, 1762
BstNI	CCWGG	3 cut(s)	707, 811, 1409
BstNSI	RCATGY	1 cut(s)	624
BstPAI	GACNNNNGTC	1 cut(s)	623
BstSCI	CCNGG	5 cut(s)	705, 742, 809, 1407, 1657
BstSFI	CTRYAG	4 cut(s)	309, 424, 759, 1734
BstSLI	GKGCMC	1 cut(s)	1758
BstUI	CGCG	3 cut(s)	1115, 1215, 1664
BstV1I	GCAGC	7 cut(s)	295, 315, 748, 767, 1205, 1321, 1324
BstX2I	RGATCY	3 cut(s)	285, 764, 1477
BstXI	CCANNNNNNTGG	1 cut(s)	1552
BstYI	RGATCY	3 cut(s)	285, 764, 1477
BsuI	GTATCC	1 cut(s)	1688
BsuRI	GGCC	7 cut(s)	413, 680, 710, 951, 1522, 1741, 1797
BtgZI	GCGATG	1 cut(s)	1050
BtsCI	GGATG	4 cut(s)	819, 840, 1595, 1696
BtsI	GCAGTG	1 cut(s)	1194
BtsIMutI	CAGTG	4 cut(s)	1059, 1194, 1395, 1604
BveI	ACCTGC	1 cut(s)	226
Cac8I	GCNNGC	4 cut(s)	678, 1373, 1758, 1789
CfoI	GCGC	3 cut(s)	308, 1217, 1664
Cfr10I	RCCGGY	2 cut(s)	951, 1233
Cfr13I	GGNCC	6 cut(s)	248, 826, 1169, 1570, 1655, 1739
CseI	GACGC	5 cut(s)	533, 565, 613, 1042, 1340
Csp6I	GTAC	3 cut(s)	159, 1030, 1246
CviAII	CATG	3 cut(s)	621, 712, 1465
CviQI	GTAC	3 cut(s)	159, 1030, 1246
DdeI	CTNAG	6 cut(s)	162, 394, 534, 684, 1120, 1173
DpnI	GATC	4 cut(s)	287, 766, 1361, 1479
DpnII	GATC	4 cut(s)	285, 764, 1359, 1477
EaeI	YGGCCR	1 cut(s)	708
Eam1104I	CTCTTC	4 cut(s)	62, 251, 456, 1043
EarI	CTCTTC	4 cut(s)	62, 251, 456, 1043
Eco130I	CCWWGG	3 cut(s)	369, 507, 921
Eco147I	AGGCCT	1 cut(s)	680
Eco31I	GGTCTC	1 cut(s)	983
Eco32I	GATATC	1 cut(s)	606
Eco47I	GGWCC	5 cut(s)	248, 826, 1169, 1570, 1655
Eco47III	AGCGCT	1 cut(s)	307
Eco57I	CTGAAG	1 cut(s)	311
Eco88I	CYCGRG	1 cut(s)	1537
EcoO109I	RGGNCCY	2 cut(s)	1570, 1739
EcoRI	GAATTC	2 cut(s)	689, 1532
EcoRII	CCWGG	3 cut(s)	705, 809, 1407
EcoRV	GATATC	1 cut(s)	606
EcoT14I	CCWWGG	3 cut(s)	369, 507, 921
EcoT22I	ATGCAT	1 cut(s)	1728
ErhI	CCWWGG	3 cut(s)	369, 507, 921
FaeI	CATG	3 cut(s)	624, 715, 1468
FalI	AAGNNNNNCTT	2 cut(s)	644, 676
FaqI	GGGAC	4 cut(s)	368, 536, 1480, 1668
FatI	CATG	3 cut(s)	620, 711, 1464
FblI	GTMKAC	1 cut(s)	627
FokI	GGATG	4 cut(s)	826, 847, 1602, 1703
FspBI	CTAG	1 cut(s)	696
GlaI	GCGC	3 cut(s)	307, 1216, 1663
HaeII	RGCGCY	1 cut(s)	309
HaeIII	GGCC	7 cut(s)	413, 680, 710, 951, 1522, 1741, 1797
HapII	CCGG	6 cut(s)	458, 743, 952, 1234, 1469, 1658
HgaI	GACGC	5 cut(s)	533, 565, 613, 1042, 1340
HhaI	GCGC	3 cut(s)	308, 1217, 1664
Hin1I	GRCGYC	2 cut(s)	525, 576
Hin1II	CATG	3 cut(s)	624, 715, 1468
Hin6I	GCGC	3 cut(s)	306, 1215, 1662
HinP1I	GCGC	3 cut(s)	306, 1215, 1662
HincII	GTYRAC	2 cut(s)	139, 628
HindII	GTYRAC	2 cut(s)	139, 628
HinfI	GANTC	7 cut(s)	554, 801, 817, 1152, 1200, 1556, 1576
HpaII	CCGG	6 cut(s)	458, 743, 952, 1234, 1469, 1658
HphI	GGTGA	3 cut(s)	613, 853, 1571
Hpy166II	GTNNAC	2 cut(s)	139, 628
Hpy188I	TCNGA	8 cut(s)	111, 122, 282, 406, 658, 1157, 1227, 1517
Hpy8I	GTNNAC	2 cut(s)	139, 628
Hpy99I	CGWCG	7 cut(s)	92, 122, 503, 629, 632, 1058, 1334
HpyCH4III	ACNGT	8 cut(s)	455, 877, 994, 1000, 1312, 1399, 1599, 1735
HpyCH4IV	ACGT	4 cut(s)	55, 870, 1020, 1365
HpyF10VI	GCNNNNNNNGC	9 cut(s)	122, 227, 395, 419, 755, 1112, 1193, 1214, 1762
HpyF3I	CTNAG	6 cut(s)	162, 394, 534, 684, 1120, 1173
HpySE526I	ACGT	4 cut(s)	55, 870, 1020, 1365
Hsp92I	GRCGYC	2 cut(s)	525, 576
Hsp92II	CATG	3 cut(s)	624, 715, 1468
HspAI	GCGC	3 cut(s)	306, 1215, 1662
Kpn2I	TCCGGA	1 cut(s)	457
KpnI	GGTACC	1 cut(s)	1249
Kzo9I	GATC	4 cut(s)	285, 764, 1359, 1477
LguI	GCTCTTC	2 cut(s)	251, 1043
LmnI	GCTCC	3 cut(s)	122, 235, 470
Lsp1109I	GCAGC	7 cut(s)	295, 315, 748, 767, 1205, 1321, 1324
LweI	GCATC	4 cut(s)	345, 493, 1002, 1189
MaeI	CTAG	1 cut(s)	696
MaeII	ACGT	4 cut(s)	55, 870, 1020, 1365
MaeIII	GTNAC	8 cut(s)	818, 871, 994, 1000, 1021, 1041, 1057, 1399
MalI	GATC	4 cut(s)	287, 766, 1361, 1479
MbiI	CCGCTC	1 cut(s)	798
MboI	GATC	4 cut(s)	285, 764, 1359, 1477
MboII	GAAGA	9 cut(s)	79, 268, 295, 473, 679, 853, 1021, 1060, 1336
MfeI	CAATTG	1 cut(s)	1563
MflI	RGATCY	3 cut(s)	285, 764, 1477
MhlI	GDGCHC	1 cut(s)	1758
MlsI	TGGCCA	1 cut(s)	710
MluCI	AATT	9 cut(s)	72, 515, 647, 689, 1453, 1499, 1532, 1563, 1704
MluNI	TGGCCA	1 cut(s)	710
MlyI	GAGTC	3 cut(s)	563, 826, 1209
MmeI	TCCRAC	2 cut(s)	100, 681
Mox20I	TGGCCA	1 cut(s)	710
Mph1103I	ATGCAT	1 cut(s)	1728
MroI	TCCGGA	1 cut(s)	457
MroXI	GAANNNNTTC	1 cut(s)	1532
MscI	TGGCCA	1 cut(s)	710
MseI	TTAA	4 cut(s)	147, 726, 969, 1810
MslI	CAYNNNNRTG	2 cut(s)	1550, 1584
Msp20I	TGGCCA	1 cut(s)	710
MspA1I	CMGCKG	1 cut(s)	588
MspI	CCGG	6 cut(s)	458, 743, 952, 1234, 1469, 1658
MspR9I	CCNGG	5 cut(s)	707, 744, 811, 1409, 1659
MunI	CAATTG	1 cut(s)	1563
Mva1269I	GAATGC	2 cut(s)	55, 374
MvaI	CCWGG	3 cut(s)	707, 811, 1409
MvnI	CGCG	3 cut(s)	1115, 1215, 1664
MwoI	GCNNNNNNNGC	9 cut(s)	122, 227, 395, 419, 755, 1112, 1193, 1214, 1762
NciI	CCSGG	2 cut(s)	744, 1659
NdeII	GATC	4 cut(s)	285, 764, 1359, 1477
NlaIII	CATG	3 cut(s)	624, 715, 1468
NlaIV	GGNNCC	6 cut(s)	124, 231, 766, 1247, 1309, 1656
NmeAIII	GCCGAG	1 cut(s)	463
NmuCI	GTSAC	7 cut(s)	818, 871, 994, 1000, 1041, 1057, 1399
NsiI	ATGCAT	1 cut(s)	1728
NspI	RCATGY	1 cut(s)	624
NspV	TTCGAA	1 cut(s)	272
PaeR7I	CTCGAG	1 cut(s)	1537
PasI	CCCWGGG	1 cut(s)	1408
PceI	AGGCCT	1 cut(s)	680
PciSI	GCTCTTC	2 cut(s)	251, 1043
PctI	GAATGC	2 cut(s)	55, 374
PdmI	GAANNNNTTC	1 cut(s)	1532
PfeI	GAWTC	4 cut(s)	801, 1152, 1556, 1576
PleI	GAGTC	3 cut(s)	562, 825, 1208
PpsI	GAGTC	3 cut(s)	562, 825, 1208
Ppu21I	YACGTR	1 cut(s)	56
PpuMI	RGGWCCY	1 cut(s)	1570
PshAI	GACNNNNGTC	1 cut(s)	623
Psp1406I	AACGTT	1 cut(s)	1020
Psp5II	RGGWCCY	1 cut(s)	1570
Psp6I	CCWGG	3 cut(s)	705, 809, 1407
PspGI	CCWGG	3 cut(s)	705, 809, 1407
PspN4I	GGNNCC	6 cut(s)	124, 231, 766, 1247, 1309, 1656
PspPI	GGNCC	6 cut(s)	248, 826, 1169, 1570, 1655, 1739
PspPPI	RGGWCCY	1 cut(s)	1570
PstI	CTGCAG	1 cut(s)	313
PsuI	RGATCY	3 cut(s)	285, 764, 1477
PvuII	CAGCTG	1 cut(s)	588
RsaI	GTAC	3 cut(s)	160, 1031, 1247
RsaNI	GTAC	3 cut(s)	159, 1030, 1246
RseI	CAYNNNNRTG	2 cut(s)	1550, 1584
SalI	GTCGAC	1 cut(s)	626
SapI	GCTCTTC	2 cut(s)	251, 1043
SaqAI	TTAA	4 cut(s)	147, 726, 969, 1810
Sau3AI	GATC	4 cut(s)	285, 764, 1359, 1477
Sau96I	GGNCC	6 cut(s)	248, 826, 1169, 1570, 1655, 1739
SchI	GAGTC	3 cut(s)	563, 826, 1209
ScrFI	CCNGG	5 cut(s)	707, 744, 811, 1409, 1659
SduI	GDGCHC	1 cut(s)	1758
SfaNI	GCATC	4 cut(s)	345, 493, 1002, 1189
SfcI	CTRYAG	4 cut(s)	309, 424, 759, 1734
Sfr274I	CTCGAG	1 cut(s)	1537
SfuI	TTCGAA	1 cut(s)	272
SgrDI	CGTCGACG	1 cut(s)	626
SinI	GGWCC	5 cut(s)	248, 826, 1169, 1570, 1655
SlaI	CTCGAG	1 cut(s)	1537
SmiMI	CAYNNNNRTG	2 cut(s)	1550, 1584
SmlI	CTYRAG	2 cut(s)	1481, 1537
SmoI	CTYRAG	2 cut(s)	1481, 1537
Sse9I	AATT	9 cut(s)	72, 515, 647, 689, 1453, 1499, 1532, 1563, 1704
SseBI	AGGCCT	1 cut(s)	680
SspMI	CTAG	1 cut(s)	696
StuI	AGGCCT	1 cut(s)	680
StyD4I	CCNGG	5 cut(s)	705, 742, 809, 1407, 1657
StyI	CCWWGG	3 cut(s)	369, 507, 921
TaaI	ACNGT	8 cut(s)	455, 877, 994, 1000, 1312, 1399, 1599, 1735
TaiI	ACGT	4 cut(s)	58, 873, 1023, 1368
TaqI	TCGA	8 cut(s)	18, 272, 498, 627, 1145, 1526, 1538, 1559
TaqII	GACCGA	2 cut(s)	59, 843
TasI	AATT	9 cut(s)	72, 515, 647, 689, 1453, 1499, 1532, 1563, 1704
TauI	GCSGC	3 cut(s)	209, 401, 777
TfiI	GAWTC	4 cut(s)	801, 1152, 1556, 1576
Tru1I	TTAA	4 cut(s)	147, 726, 969, 1810
Tru9I	TTAA	4 cut(s)	147, 726, 969, 1810
TscAI	CASTG	4 cut(s)	1066, 1194, 1402, 1604
TseFI	GTSAC	7 cut(s)	818, 871, 994, 1000, 1041, 1057, 1399
TseI	GCWGC	7 cut(s)	303, 308, 736, 755, 1193, 1334, 1337
Tsp45I	GTSAC	7 cut(s)	818, 871, 994, 1000, 1041, 1057, 1399
TspDTI	ATGAA	4 cut(s)	121, 367, 867, 1309
TspRI	CASTG	4 cut(s)	1066, 1194, 1402, 1604
VpaK11BI	GGWCC	5 cut(s)	248, 826, 1169, 1570, 1655
XapI	RAATTY	2 cut(s)	689, 1532
XceI	RCATGY	1 cut(s)	624
XcmI	CCANNNNNNNNNTGG	1 cut(s)	874
XhoI	CTCGAG	1 cut(s)	1537
XmiI	GTMKAC	1 cut(s)	627
XmnI	GAANNNNTTC	1 cut(s)	1532
XspI	CTAG	1 cut(s)	696
Zsp2I	ATGCAT	1 cut(s)	1728

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.