FvH4_1g10040

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0019650
Physical Location & Seq
Reverse (-)
5448793 .. 5449941
1149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g10040.t1

Sequence Viewer

Length: 693 bp
ATGAAGTCGGCAATGTTGTCATTTCTCATTGTTTTGCTTAGTGTTCAAATGCTGCTAGCAGCAAATCCACCTCCACCTCCACCTCCAGCTGTTCCAGCTCTTGATTCATACAAGCTGGTCATTGAATGGCCAAACACTTTTTGTCTTGTTGAGCCACAGCCACCATGCCAACAGCATCCACAGTCTTTTACATTGCATGGCCTTTGGCCACAGGCGGGTGGTCGATCGTTAGTGAACTGTCCTGGTTCACCAATGGAAGATCAAACCCTAGAGGCAAATAAAGGTGATCTGACAAAGTTTTGGCCTGATCTTAGGAACTCGGACTTTGATAAGAGTAAAAGCTTTTGGCGTCAGGAGTGGGACACACACGGTCGCTGTTCTGGCAAGCCTCCGGCCGACTACCTTACCATGGTCTTTAATGCAGTAAAAAAGTATGACGTGCAAAAGTTACTTGCAAAGAATGGAATTTTACCAGGTGATCCGACAGGGCATACGGCGGCACAGTTTGAACAGGCAATATTCAAAGAAACTAAACTGCACACAGAGATTGAATGCCGGACGAAGGACTCGAAGGAGTATCTATTTCAAATTTATTTCTGCTTAACACCTCAAGGACAGTTCAAGAATTGTCCGATCCGTTACAGGTGTGCAAATCAACAAATCTACATGCCCTTGCCTCCGCCCAGTCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

231

Amino Acids

26.02

Weight (kDa)

8.08

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 36 - 210 6.7e-38 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10040
rosa_chinensis RchiOBHm_Chr2g0095681
rosa_laevigata RLG00000016585
rosa_multiflora Rmu_sc0001309.1_g000009 Rmu_ssc0000215.1_g000027
rosa_samantha Rh2AG108600 Rh2DG102100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 215, 497, 680
AclWI GGATC 2 cut(s) 473, 628
AcoI YGGCCR 3 cut(s) 128, 206, 393
AcsI RAATTY 2 cut(s) 465, 588
AcyI GRCGYC 1 cut(s) 349
AfiI CCNNNNNNNGG 3 cut(s) 215, 409, 562
AgsI TTSAA 7 cut(s) 47, 125, 509, 523, 551, 587, 622
AjiI CACGTC 1 cut(s) 439
AjnI CCWGG 2 cut(s) 241, 472
AluBI AGCT 4 cut(s) 89, 98, 115, 342
AluI AGCT 4 cut(s) 89, 98, 115, 342
AlwI GGATC 2 cut(s) 473, 628
AoxI GGCC 5 cut(s) 128, 199, 206, 302, 393
ApeKI GCWGC 2 cut(s) 52, 59
ApoI RAATTY 2 cut(s) 465, 588
AsuHPI GGTGA 3 cut(s) 240, 296, 488
AsuNHI GCTAGC 1 cut(s) 55
BalI TGGCCA 2 cut(s) 130, 208
BbvI GCAGC 2 cut(s) 39, 71
BceAI ACGGC 1 cut(s) 510
BciT130I CCWGG 2 cut(s) 243, 474
BfaI CTAG 3 cut(s) 56, 269, 691
BisI GCNGC 3 cut(s) 53, 60, 498
BlsI GCNGC 3 cut(s) 54, 61, 499
Bme1390I CCNGG 2 cut(s) 243, 474
BmgBI CACGTC 1 cut(s) 439
BmrFI CCNGG 2 cut(s) 243, 474
BmrI ACTGGG 1 cut(s) 678
BmsI GCATC 1 cut(s) 184
BmtI GCTAGC 1 cut(s) 59
BmuI ACTGGG 1 cut(s) 678
BpmI CTGGAG 1 cut(s) 69
BpuEI CTTGAG 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 349
BsaJI CCNNGG 1 cut(s) 408
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 409, 562
Bse1I ACTGG 1 cut(s) 684
Bse3DI GCAATG 2 cut(s) 18, 191
BseBI CCWGG 2 cut(s) 243, 474
BseDI CCNNGG 1 cut(s) 408
BseGI GGATG 1 cut(s) 175
BseLI CCNNNNNNNGG 3 cut(s) 215, 409, 562
BseMI GCAATG 2 cut(s) 18, 191
BseNI ACTGG 1 cut(s) 684
BseX3I CGGCCG 1 cut(s) 393
BseXI GCAGC 2 cut(s) 39, 71
BsgI GTGCAG 1 cut(s) 521
Bsh1285I CGRYCG 3 cut(s) 227, 373, 396
BshFI GGCC 5 cut(s) 130, 201, 208, 304, 395
BsiEI CGRYCG 3 cut(s) 227, 373, 396
BsiSI CCGG 2 cut(s) 392, 556
BslFI GGGAC 2 cut(s) 374, 672
BslI CCNNNNNNNGG 3 cut(s) 215, 409, 562
BsmFI GGGAC 2 cut(s) 374, 672
BsmI GAATGC 1 cut(s) 557
BsnI GGCC 5 cut(s) 130, 201, 208, 304, 395
Bsp143I GATC 6 cut(s) 224, 259, 286, 307, 478, 633
Bsp19I CCATGG 1 cut(s) 408
BspACI CCGC 3 cut(s) 215, 497, 680
BspANI GGCC 5 cut(s) 130, 201, 208, 304, 395
BspOI GCTAGC 1 cut(s) 59
BspPI GGATC 2 cut(s) 473, 628
BsrDI GCAATG 2 cut(s) 18, 191
BsrI ACTGG 1 cut(s) 684
BssECI CCNNGG 1 cut(s) 408
BssMI GATC 6 cut(s) 224, 259, 286, 307, 478, 633
BssNI GRCGYC 1 cut(s) 349
BssT1I CCWWGG 1 cut(s) 408
Bst2UI CCWGG 2 cut(s) 243, 474
Bst4CI ACNGT 5 cut(s) 183, 239, 371, 504, 618
BstACI GRCGYC 1 cut(s) 349
BstC8I GCNNGC 2 cut(s) 57, 386
BstDEI CTNAG 2 cut(s) 38, 311
BstDSI CCRYGG 1 cut(s) 408
BstF5I GGATG 1 cut(s) 175
BstKTI GATC 6 cut(s) 227, 262, 289, 310, 481, 636
BstMBI GATC 6 cut(s) 224, 259, 286, 307, 478, 633
BstMCI CGRYCG 3 cut(s) 227, 373, 396
BstMWI GCNNNNNNNGC 2 cut(s) 95, 381
BstNI CCWGG 2 cut(s) 243, 474
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 241, 472
BstV1I GCAGC 2 cut(s) 39, 71
BstZI CGGCCG 1 cut(s) 393
BsuRI GGCC 5 cut(s) 130, 201, 208, 304, 395
BtgI CCRYGG 1 cut(s) 408
BtrI CACGTC 1 cut(s) 439
BtsCI GGATG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 57, 386
CseI GACGC 1 cut(s) 338
CsiI ACCWGGT 1 cut(s) 472
CviAII CATG 4 cut(s) 165, 197, 409, 667
DdeI CTNAG 2 cut(s) 38, 311
DpnI GATC 6 cut(s) 226, 261, 288, 309, 480, 635
DpnII GATC 6 cut(s) 224, 259, 286, 307, 478, 633
EaeI YGGCCR 3 cut(s) 128, 206, 393
EagI CGGCCG 1 cut(s) 393
EciI GGCGGA 1 cut(s) 669
EclXI CGGCCG 1 cut(s) 393
Eco130I CCWWGG 1 cut(s) 408
Eco52I CGGCCG 1 cut(s) 393
EcoRII CCWGG 2 cut(s) 241, 472
EcoT14I CCWWGG 1 cut(s) 408
ErhI CCWWGG 1 cut(s) 408
FaeI CATG 4 cut(s) 168, 200, 412, 670
FaiI YATR 7 cut(s) 109, 166, 198, 410, 435, 492, 668
FaqI GGGAC 2 cut(s) 374, 672
FatI CATG 4 cut(s) 164, 196, 408, 666
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 53, 60, 498
FokI GGATG 1 cut(s) 162
Fsp4HI GCNGC 3 cut(s) 53, 60, 498
FspBI CTAG 3 cut(s) 56, 269, 691
GluI GCNGC 3 cut(s) 53, 60, 498
GsuI CTGGAG 1 cut(s) 69
HaeIII GGCC 5 cut(s) 130, 201, 208, 304, 395
HapII CCGG 2 cut(s) 392, 556
HgaI GACGC 1 cut(s) 338
Hin1I GRCGYC 1 cut(s) 349
Hin1II CATG 4 cut(s) 168, 200, 412, 670
HindIII AAGCTT 1 cut(s) 340
HinfI GANTC 2 cut(s) 104, 566
HpaII CCGG 2 cut(s) 392, 556
HphI GGTGA 3 cut(s) 240, 296, 488
Hpy166II GTNNAC 2 cut(s) 235, 248
Hpy188I TCNGA 4 cut(s) 291, 322, 483, 633
Hpy188III TCNNGA 3 cut(s) 101, 353, 622
Hpy8I GTNNAC 2 cut(s) 235, 248
HpyAV CCTTC 2 cut(s) 556, 565
HpyCH4III ACNGT 5 cut(s) 183, 239, 371, 504, 618
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 6 cut(s) 196, 422, 442, 455, 538, 650
HpyF10VI GCNNNNNNNGC 2 cut(s) 95, 381
HpyF3I CTNAG 2 cut(s) 38, 311
HpySE526I ACGT 1 cut(s) 438
Hsp92I GRCGYC 1 cut(s) 349
Hsp92II CATG 4 cut(s) 168, 200, 412, 670
Kzo9I GATC 6 cut(s) 224, 259, 286, 307, 478, 633
Lsp1109I GCAGC 2 cut(s) 39, 71
LweI GCATC 1 cut(s) 184
MabI ACCWGGT 1 cut(s) 472
MaeI CTAG 3 cut(s) 56, 269, 691
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 447, 638
MalI GATC 6 cut(s) 226, 261, 288, 309, 480, 635
MboI GATC 6 cut(s) 224, 259, 286, 307, 478, 633
MboII GAAGA 1 cut(s) 269
MlsI TGGCCA 2 cut(s) 130, 208
MluCI AATT 3 cut(s) 465, 588, 625
MluNI TGGCCA 2 cut(s) 130, 208
MlyI GAGTC 1 cut(s) 560
MmeI TCCRAC 1 cut(s) 506
MnlI CCTC 7 cut(s) 81, 87, 93, 265, 399, 618, 687
Mox20I TGGCCA 2 cut(s) 130, 208
MscI TGGCCA 2 cut(s) 130, 208
MseI TTAA 2 cut(s) 417, 602
Msp20I TGGCCA 2 cut(s) 130, 208
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 392, 556
MspR9I CCNGG 2 cut(s) 243, 474
Mva1269I GAATGC 1 cut(s) 557
MvaI CCWGG 2 cut(s) 243, 474
MwoI GCNNNNNNNGC 2 cut(s) 95, 381
NcoI CCATGG 1 cut(s) 408
NdeII GATC 6 cut(s) 224, 259, 286, 307, 478, 633
NheI GCTAGC 1 cut(s) 55
NlaIII CATG 4 cut(s) 168, 200, 412, 670
NspI RCATGY 1 cut(s) 670
PcsI WCGNNNNNNNCGW 1 cut(s) 566
PctI GAATGC 1 cut(s) 557
PfeI GAWTC 1 cut(s) 104
PkrI GCNGC 3 cut(s) 54, 61, 499
Ple19I CGATCG 1 cut(s) 227
PleI GAGTC 1 cut(s) 560
PpsI GAGTC 1 cut(s) 560
Psp6I CCWGG 2 cut(s) 241, 472
PspGI CCWGG 2 cut(s) 241, 472
PvuI CGATCG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 89
SaqAI TTAA 2 cut(s) 417, 602
SatI GCNGC 3 cut(s) 53, 60, 498
Sau3AI GATC 6 cut(s) 224, 259, 286, 307, 478, 633
SchI GAGTC 1 cut(s) 560
ScrFI CCNGG 2 cut(s) 243, 474
SexAI ACCWGGT 1 cut(s) 472
SfaNI GCATC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 609
SmoI CTYRAG 1 cut(s) 609
Sse9I AATT 3 cut(s) 465, 588, 625
SsiI CCGC 3 cut(s) 215, 497, 680
SspI AATATT 1 cut(s) 519
SspMI CTAG 3 cut(s) 56, 269, 691
StyD4I CCNGG 2 cut(s) 241, 472
StyI CCWWGG 1 cut(s) 408
TaaI ACNGT 5 cut(s) 183, 239, 371, 504, 618
TaiI ACGT 1 cut(s) 441
TaqI TCGA 2 cut(s) 223, 569
TasI AATT 3 cut(s) 465, 588, 625
TauI GCSGC 1 cut(s) 500
TfiI GAWTC 1 cut(s) 104
Tru1I TTAA 2 cut(s) 417, 602
Tru9I TTAA 2 cut(s) 417, 602
TseI GCWGC 2 cut(s) 52, 59
TspDTI ATGAA 2 cut(s) 17, 96
TspGWI ACGGA 1 cut(s) 626
XapI RAATTY 2 cut(s) 465, 588
XceI RCATGY 1 cut(s) 670
XspI CTAG 3 cut(s) 56, 269, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.