Rh2AG108600

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0019650
Physical Location & Seq
Reverse (-)
9589697 .. 9602973
13277 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG108600.1

Sequence Viewer

Length: 831 bp
ATGGTTCAACGTACTGATGAAGCAGACGAAGAAATTTCCAGCTATGAAGATGTTGGTGAAGCCTTTGTGCGTGAAAATGAGGAGAATGAAATGGATTGTGAGGTTTTGCAGAGCTTGGAGGAACAACATTTTGGTGTTAATGAGGATTCCGTGCACCAACCTGTGCGAATGCCCTCGAGAATCAAACCGTCCATGAGTCCGGAGCCTCTCATACTTGGAAACAAGTTCAAAACAAGAGGTGTCTTAAGGCCTATTGAAACTGAATCATATGTCCCAGTGGTGGAAGCATTGCTTCGCTTAAATGCACAGAGCCAGCCAGATAGTTTCTTGATTGAGATCGATTTGCTAAAACCCTCTCTTCACCACCTCATCACCACCATGAAGTCGATTTTGATTTCATTTGTCACAATTTGTATCAGTTTTCAACTTCTCCTATCAGCGCCAAATCCACCTCAAGCCCCACCTCCTGATTCCTTCCTTTTGGTCATTGTGTGGCCCAATACCTTCTGTCTTTTTGAGTCATGTCAGCTGCATCCACAATCTTTTACACTACATGGATTGTGGCCACAGGCGAATGGGAAATCCTTGGTGGACTGTGCTGGTTCAGAAATGGATGATAGCACCTTAAATGGCAAGAAAGATGATTTGAACAAGTATTGGCCAGACTTGACGCATTCCAAGTTTGAAGAGAGTAAAAGCTTTTGGATACATGAGTGGGAAAAACATGGTCGTTGCTCTGCAAAGAGTCCTGCTAATTACTTGAGCCTGGTGTTTGATCTGATGAAAAAACATGATGTGGAACAGATATTTAAAAACAATGGTAAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

276

Amino Acids

31.47

Weight (kDa)

5.08

Isoelectric Point (pI)

53.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 157 - 274 4.6e-26 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0019650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10040
rosa_chinensis RchiOBHm_Chr2g0095681
rosa_laevigata RLG00000016585
rosa_multiflora Rmu_sc0001309.1_g000009 Rmu_ssc0000215.1_g000027
rosa_samantha Rh2AG108600 Rh2DG102100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 199
AcoI YGGCCR 2 cut(s) 563, 659
AcsI RAATTY 1 cut(s) 33
AfaI GTAC 1 cut(s) 13
AfiI CCNNNNNNNGG 1 cut(s) 280
AflII CTTAAG 1 cut(s) 244
AgsI TTSAA 6 cut(s) 8, 229, 257, 425, 649, 686
AjnI CCWGG 1 cut(s) 765
AjuI GAANNNNNNNTTGG 2 cut(s) 114, 146
AluBI AGCT 4 cut(s) 42, 114, 529, 699
AluI AGCT 4 cut(s) 42, 114, 529, 699
Alw21I GWGCWC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 152
Ama87I CYCGRG 1 cut(s) 175
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 4 cut(s) 248, 494, 563, 659
ApaLI GTGCAC 1 cut(s) 152
ApeKI GCWGC 1 cut(s) 529
ApoI RAATTY 1 cut(s) 33
AspLEI GCGC 1 cut(s) 442
AspS9I GGNCC 1 cut(s) 495
AsuHPI GGTGA 3 cut(s) 68, 353, 364
AvaI CYCGRG 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 156
BaeI ACNNNNGTAYC 2 cut(s) 698, 731
BalI TGGCCA 2 cut(s) 565, 661
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 516
BciT130I CCWGG 1 cut(s) 767
BciVI GTATCC 1 cut(s) 699
BfoI RGCGCY 1 cut(s) 443
BfrI CTTAAG 1 cut(s) 244
BfuI GTATCC 1 cut(s) 699
BisI GCNGC 1 cut(s) 530
BlsI GCNGC 1 cut(s) 531
Bme1390I CCNGG 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 495
BmiI GGNNCC 1 cut(s) 204
BmrFI CCNGG 1 cut(s) 767
BmrI ACTGGG 1 cut(s) 269
BmsI GCATC 1 cut(s) 541
BmuI ACTGGG 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 438, 781
Bsa29I ATCGAT 1 cut(s) 339
BsaBI GATNNNNATC 1 cut(s) 335
BsaJI CCNNGG 1 cut(s) 585
BsaWI WCCGGW 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse1I ACTGG 1 cut(s) 275
Bse3DI GCAATG 1 cut(s) 287
Bse8I GATNNNNATC 1 cut(s) 335
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 1 cut(s) 767
BseCI ATCGAT 1 cut(s) 339
BseDI CCNNGG 1 cut(s) 585
BseGI GGATG 2 cut(s) 532, 619
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMI GCAATG 1 cut(s) 287
BseNI ACTGG 1 cut(s) 275
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 1 cut(s) 516
BshFI GGCC 4 cut(s) 250, 496, 565, 661
BshVI ATCGAT 1 cut(s) 339
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 175
BsiSI CCGG 1 cut(s) 200
BslFI GGGAC 1 cut(s) 257
BslI CCNNNNNNNGG 1 cut(s) 280
BsmFI GGGAC 1 cut(s) 257
BsmI GAATGC 2 cut(s) 174, 673
BsnI GGCC 4 cut(s) 250, 496, 565, 661
BsoBI CYCGRG 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 156
Bsp13I TCCGGA 1 cut(s) 199
Bsp143I GATC 2 cut(s) 336, 775
BspANI GGCC 4 cut(s) 250, 496, 565, 661
BspDI ATCGAT 1 cut(s) 339
BspEI TCCGGA 1 cut(s) 199
BspLI GGNNCC 1 cut(s) 204
BspTI CTTAAG 1 cut(s) 244
BsrDI GCAATG 1 cut(s) 287
BsrI ACTGG 1 cut(s) 275
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 2 cut(s) 336, 775
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 767
Bst4CI ACNGT 2 cut(s) 189, 596
Bst6I CTCTTC 2 cut(s) 363, 681
BstAFI CTTAAG 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 314
BstF5I GGATG 2 cut(s) 532, 619
BstH2I RGCGCY 1 cut(s) 443
BstHHI GCGC 1 cut(s) 442
BstKTI GATC 2 cut(s) 339, 778
BstMBI GATC 2 cut(s) 336, 775
BstNI CCWGG 1 cut(s) 767
BstSCI CCNGG 1 cut(s) 765
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 516
Bsu15I ATCGAT 1 cut(s) 339
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 4 cut(s) 250, 496, 565, 661
BsuTUI ATCGAT 1 cut(s) 339
BtsCI GGATG 2 cut(s) 532, 619
BtsIMutI CAGTG 1 cut(s) 282
Cac8I GCNNGC 1 cut(s) 314
CfoI GCGC 1 cut(s) 442
Cfr13I GGNCC 1 cut(s) 495
ClaI ATCGAT 1 cut(s) 339
CseI GACGC 1 cut(s) 679
Csp6I GTAC 1 cut(s) 12
CviAII CATG 7 cut(s) 193, 379, 522, 554, 710, 725, 791
CviQI GTAC 1 cut(s) 12
DpnI GATC 2 cut(s) 338, 777
DpnII GATC 2 cut(s) 336, 775
DraI TTTAAA 1 cut(s) 811
EaeI YGGCCR 2 cut(s) 563, 659
Eam1104I CTCTTC 2 cut(s) 363, 681
EarI CTCTTC 2 cut(s) 363, 681
Eco130I CCWWGG 1 cut(s) 585
Eco147I AGGCCT 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 765
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
FaeI CATG 7 cut(s) 196, 382, 525, 557, 713, 728, 794
FalI AAGNNNNNCTT 2 cut(s) 276, 308
FaqI GGGAC 1 cut(s) 257
FatI CATG 7 cut(s) 192, 378, 521, 553, 709, 724, 790
FauNDI CATATG 1 cut(s) 268
Fnu4HI GCNGC 1 cut(s) 530
FokI GGATG 2 cut(s) 519, 626
Fsp4HI GCNGC 1 cut(s) 530
GlaI GCGC 1 cut(s) 441
GluI GCNGC 1 cut(s) 530
HaeII RGCGCY 1 cut(s) 443
HaeIII GGCC 4 cut(s) 250, 496, 565, 661
HapII CCGG 1 cut(s) 200
HgaI GACGC 1 cut(s) 679
HhaI GCGC 1 cut(s) 442
Hin1II CATG 7 cut(s) 196, 382, 525, 557, 713, 728, 794
Hin6I GCGC 1 cut(s) 440
HinP1I GCGC 1 cut(s) 440
HindIII AAGCTT 1 cut(s) 697
HinfI GANTC 7 cut(s) 146, 180, 196, 263, 470, 518, 745
HpaII CCGG 1 cut(s) 200
HphI GGTGA 3 cut(s) 68, 353, 364
Hpy166II GTNNAC 2 cut(s) 154, 592
Hpy188I TCNGA 2 cut(s) 607, 780
Hpy188III TCNNGA 4 cut(s) 177, 200, 328, 467
Hpy8I GTNNAC 2 cut(s) 154, 592
HpyAV CCTTC 2 cut(s) 484, 514
HpyCH4III ACNGT 2 cut(s) 189, 596
HpyCH4IV ACGT 1 cut(s) 10
HpyCH4V TGCA 5 cut(s) 109, 154, 305, 532, 740
HpySE526I ACGT 1 cut(s) 10
Hsp92II CATG 7 cut(s) 196, 382, 525, 557, 713, 728, 794
HspAI GCGC 1 cut(s) 440
Kpn2I TCCGGA 1 cut(s) 199
Kzo9I GATC 2 cut(s) 336, 775
LmnI GCTCC 1 cut(s) 202
Lsp1109I GCAGC 1 cut(s) 516
LweI GCATC 1 cut(s) 541
MaeII ACGT 1 cut(s) 10
MaeIII GTNAC 1 cut(s) 403
MalI GATC 2 cut(s) 338, 777
MboI GATC 2 cut(s) 336, 775
MboII GAAGA 4 cut(s) 41, 59, 350, 698
MhlI GDGCHC 1 cut(s) 156
MlsI TGGCCA 2 cut(s) 565, 661
MluCI AATT 3 cut(s) 33, 408, 754
MluNI TGGCCA 2 cut(s) 565, 661
MlyI GAGTC 3 cut(s) 205, 527, 754
Mox20I TGGCCA 2 cut(s) 565, 661
MroI TCCGGA 1 cut(s) 199
MscI TGGCCA 2 cut(s) 565, 661
MseI TTAA 5 cut(s) 138, 245, 299, 626, 810
MslI CAYNNNNRTG 2 cut(s) 132, 377
Msp20I TGGCCA 2 cut(s) 565, 661
MspA1I CMGCKG 1 cut(s) 529
MspCI CTTAAG 1 cut(s) 244
MspI CCGG 1 cut(s) 200
MspR9I CCNGG 1 cut(s) 767
Mva1269I GAATGC 2 cut(s) 174, 673
MvaI CCWGG 1 cut(s) 767
NdeI CATATG 1 cut(s) 268
NdeII GATC 2 cut(s) 336, 775
NlaIII CATG 7 cut(s) 196, 382, 525, 557, 713, 728, 794
NlaIV GGNNCC 1 cut(s) 204
NmuCI GTSAC 1 cut(s) 403
PaeR7I CTCGAG 1 cut(s) 175
PceI AGGCCT 1 cut(s) 250
PctI GAATGC 2 cut(s) 174, 673
PfeI GAWTC 4 cut(s) 146, 180, 263, 470
PkrI GCNGC 1 cut(s) 531
PleI GAGTC 3 cut(s) 204, 526, 753
PpsI GAGTC 3 cut(s) 204, 526, 753
Psp6I CCWGG 1 cut(s) 765
PspGI CCWGG 1 cut(s) 765
PspN4I GGNNCC 1 cut(s) 204
PspPI GGNCC 1 cut(s) 495
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 1 cut(s) 13
RsaNI GTAC 1 cut(s) 12
RseI CAYNNNNRTG 2 cut(s) 132, 377
SaqAI TTAA 5 cut(s) 138, 245, 299, 626, 810
SatI GCNGC 1 cut(s) 530
Sau3AI GATC 2 cut(s) 336, 775
Sau96I GGNCC 1 cut(s) 495
SchI GAGTC 3 cut(s) 205, 527, 754
ScrFI CCNGG 1 cut(s) 767
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 1 cut(s) 541
Sfr274I CTCGAG 1 cut(s) 175
SlaI CTCGAG 1 cut(s) 175
SmiMI CAYNNNNRTG 2 cut(s) 132, 377
SmlI CTYRAG 4 cut(s) 175, 244, 453, 760
SmoI CTYRAG 4 cut(s) 175, 244, 453, 760
Sse9I AATT 3 cut(s) 33, 408, 754
SseBI AGGCCT 1 cut(s) 250
StuI AGGCCT 1 cut(s) 250
StyD4I CCNGG 1 cut(s) 765
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 2 cut(s) 189, 596
TaiI ACGT 1 cut(s) 13
TaqI TCGA 3 cut(s) 176, 339, 386
TasI AATT 3 cut(s) 33, 408, 754
TfiI GAWTC 4 cut(s) 146, 180, 263, 470
Tru1I TTAA 5 cut(s) 138, 245, 299, 626, 810
Tru9I TTAA 5 cut(s) 138, 245, 299, 626, 810
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 529
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 6 cut(s) 33, 60, 102, 387, 395, 797
TspGWI ACGGA 1 cut(s) 139
TspRI CASTG 1 cut(s) 282
Vha464I CTTAAG 1 cut(s) 244
VneI GTGCAC 1 cut(s) 152
XapI RAATTY 1 cut(s) 33
XhoI CTCGAG 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.