RLG00000016585

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0019650
Physical Location & Seq
Forward (+)
7953134 .. 7954041
908 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016585

Sequence Viewer

Length: 702 bp
ATGAAGTCGATGATATCTTCATTTCTCACCATTTTTCTCTGTTTTCAACTGCTCCTAGCAGTGCCAAATCCACCTCCAGCGAAAATTTCAGCACCTGATTCCTTCCTGTTGGCGGAAGGTTCCTCGTTGAAATGTACGAGTGTACCAATGATTGATAGCATCTTAAAAGGCAAGAAAGATGATTTGGAAAGGTATTGGCCAAACTTGAAGCACACAAAGTTCGATGAGAGTAAAAAATTCTGGATTAGTGAGTGGATTAAACATGGTAGCTGCTCCGCAAAGACTCCTGCTAATTACTTGAGCCTGGTTTTTGATCTAATGAAGAAAATCAAGAAATTTGATGTGAAAAAGATATTTGAAAAGCATGGAATTTTACCAAGTGATCAGAGAGAGTATCAGGCGATAGAATTTGCAGAGGCAATATTTGACGAAACTAAATTCTGGACAGGGATCGAATGCATATCGAAGTTCGACAGTAAGGGGCAGCATCTATTTCAAATTTATTTCTGCTTAACAGCTCAAGCAAAATTCAAGAATTGTTCGGACGATCGTGCTTTCTATAAAAACTGCCAAAAGTCTTTGACAGTCATCTTCCCCTTGCCTCCGCCCAGTCTCATGGTATCTGCAAATGAAGAAGAGAGAACCAATGCGGCCCAGAAGCTTCTAAAGGGAGAAGATCGAGACGTGAAAAGCAAGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

234

Amino Acids

26.7

Weight (kDa)

9.02

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 44 - 180 4.3e-22 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0019650)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g10040
rosa_chinensis RchiOBHm_Chr2g0095681
rosa_laevigata RLG00000016585
rosa_multiflora Rmu_sc0001309.1_g000009 Rmu_ssc0000215.1_g000027
rosa_samantha Rh2AG108600 Rh2DG102100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 113, 276, 605, 650
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 197
AcsI RAATTY 8 cut(s) 84, 236, 335, 369, 407, 437, 498, 527
AfaI GTAC 2 cut(s) 136, 144
AfiI CCNNNNNNNGG 1 cut(s) 112
AgsI TTSAA 6 cut(s) 47, 130, 208, 359, 497, 532
AjiI CACGTC 1 cut(s) 685
AjnI CCWGG 1 cut(s) 303
AjuI GAANNNNNNNTTGG 2 cut(s) 167, 199
AluBI AGCT 3 cut(s) 270, 518, 661
AluI AGCT 3 cut(s) 270, 518, 661
Alw26I GTCTC 2 cut(s) 617, 675
AlwI GGATC 1 cut(s) 458
AlwNI CAGNNNCTG 1 cut(s) 95
AoxI GGCC 2 cut(s) 197, 651
ApeKI GCWGC 2 cut(s) 270, 484
ApoI RAATTY 8 cut(s) 84, 236, 335, 369, 407, 437, 498, 527
AspS9I GGNCC 1 cut(s) 652
AsuHPI GGTGA 1 cut(s) 19
BaeI ACNNNNGTAYC 2 cut(s) 126, 159
BalI TGGCCA 1 cut(s) 199
BbvI GCAGC 2 cut(s) 257, 496
BciT130I CCWGG 1 cut(s) 305
BclI TGATCA 1 cut(s) 382
BcoDI GTCTC 2 cut(s) 617, 675
BfaI CTAG 1 cut(s) 56
BisI GCNGC 3 cut(s) 271, 485, 651
BlsI GCNGC 3 cut(s) 272, 486, 652
Bme1390I CCNGG 1 cut(s) 305
BmgBI CACGTC 1 cut(s) 685
BmgT120I GGNCC 1 cut(s) 652
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 305
BmrI ACTGGG 1 cut(s) 603
BmsI GCATC 2 cut(s) 168, 496
BmuI ACTGGG 1 cut(s) 603
BpmI CTGGAG 1 cut(s) 60
BpuEI CTTGAG 2 cut(s) 319, 504
Bsc4I CCNNNNNNNGG 1 cut(s) 112
Bse1I ACTGG 1 cut(s) 609
BseBI CCWGG 1 cut(s) 305
BseLI CCNNNNNNNGG 1 cut(s) 112
BseNI ACTGG 1 cut(s) 609
BseXI GCAGC 2 cut(s) 257, 496
Bsh1285I CGRYCG 1 cut(s) 550
BshFI GGCC 2 cut(s) 199, 653
BsiEI CGRYCG 1 cut(s) 550
BslI CCNNNNNNNGG 1 cut(s) 112
BsmAI GTCTC 2 cut(s) 617, 675
BsmBI CGTCTC 1 cut(s) 675
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 199, 653
Bsp143I GATC 5 cut(s) 313, 382, 450, 547, 676
BspACI CCGC 4 cut(s) 113, 276, 605, 650
BspANI GGCC 2 cut(s) 199, 653
BspLI GGNNCC 1 cut(s) 121
BspPI GGATC 1 cut(s) 458
BsrI ACTGG 1 cut(s) 609
BssMI GATC 5 cut(s) 313, 382, 450, 547, 676
Bst2UI CCWGG 1 cut(s) 305
Bst4CI ACNGT 2 cut(s) 476, 586
Bst6I CTCTTC 1 cut(s) 630
BstKTI GATC 5 cut(s) 316, 385, 453, 550, 679
BstMAI GTCTC 2 cut(s) 617, 675
BstMBI GATC 5 cut(s) 313, 382, 450, 547, 676
BstMCI CGRYCG 1 cut(s) 550
BstNI CCWGG 1 cut(s) 305
BstSCI CCNGG 1 cut(s) 303
BstV1I GCAGC 2 cut(s) 257, 496
BstXI CCANNNNNNTGG 1 cut(s) 616
BsuRI GGCC 2 cut(s) 199, 653
BtrI CACGTC 1 cut(s) 685
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
CaiI CAGNNNCTG 1 cut(s) 95
Cfr13I GGNCC 1 cut(s) 652
Csp6I GTAC 2 cut(s) 135, 143
CviAII CATG 3 cut(s) 263, 365, 616
CviJI RGCY 6 cut(s) 199, 270, 303, 518, 653, 661
CviKI_1 RGCY 6 cut(s) 199, 270, 303, 518, 653, 661
CviQI GTAC 2 cut(s) 135, 143
DpnI GATC 5 cut(s) 315, 384, 452, 549, 678
DpnII GATC 5 cut(s) 313, 382, 450, 547, 676
EaeI YGGCCR 1 cut(s) 197
Eam1104I CTCTTC 1 cut(s) 630
EarI CTCTTC 1 cut(s) 630
EciI GGCGGA 2 cut(s) 128, 594
Eco32I GATATC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 303
EcoRV GATATC 1 cut(s) 15
EcoT22I ATGCAT 1 cut(s) 461
Esp3I CGTCTC 1 cut(s) 675
FaeI CATG 3 cut(s) 266, 368, 619
FaiI YATR 5 cut(s) 264, 366, 461, 561, 617
FatI CATG 3 cut(s) 262, 364, 615
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 3 cut(s) 271, 485, 651
Fsp4HI GCNGC 3 cut(s) 271, 485, 651
FspBI CTAG 1 cut(s) 56
GluI GCNGC 3 cut(s) 271, 485, 651
GsuI CTGGAG 1 cut(s) 60
HaeIII GGCC 2 cut(s) 199, 653
Hin1II CATG 3 cut(s) 266, 368, 619
HindIII AAGCTT 1 cut(s) 659
HinfI GANTC 2 cut(s) 98, 283
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 2 cut(s) 387, 544
Hpy188III TCNNGA 5 cut(s) 241, 331, 442, 532, 680
Hpy8I GTNNAC 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 110, 112
HpyCH4III ACNGT 2 cut(s) 476, 586
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 3 cut(s) 413, 459, 626
HpySE526I ACGT 1 cut(s) 684
Hsp92II CATG 3 cut(s) 266, 368, 619
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 5 cut(s) 313, 382, 450, 547, 676
LmnI GCTCC 2 cut(s) 57, 278
Lsp1109I GCAGC 2 cut(s) 257, 496
LweI GCATC 2 cut(s) 168, 496
MaeI CTAG 1 cut(s) 56
MaeII ACGT 1 cut(s) 684
MalI GATC 5 cut(s) 315, 384, 452, 549, 678
MboI GATC 5 cut(s) 313, 382, 450, 547, 676
MboII GAAGA 6 cut(s) 9, 334, 583, 644, 647, 686
MlsI TGGCCA 1 cut(s) 199
MluNI TGGCCA 1 cut(s) 199
MlyI GAGTC 1 cut(s) 277
MnlI CCTC 4 cut(s) 84, 133, 409, 612
Mox20I TGGCCA 1 cut(s) 199
Mph1103I ATGCAT 1 cut(s) 461
MscI TGGCCA 1 cut(s) 199
MseI TTAA 3 cut(s) 164, 258, 512
Msp20I TGGCCA 1 cut(s) 199
MspR9I CCNGG 1 cut(s) 305
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 1 cut(s) 305
NdeII GATC 5 cut(s) 313, 382, 450, 547, 676
NlaIII CATG 3 cut(s) 266, 368, 619
NlaIV GGNNCC 1 cut(s) 121
NsiI ATGCAT 1 cut(s) 461
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 1 cut(s) 98
PkrI GCNGC 3 cut(s) 272, 486, 652
Ple19I CGATCG 1 cut(s) 550
PleI GAGTC 1 cut(s) 277
PpsI GAGTC 1 cut(s) 277
Psp6I CCWGG 1 cut(s) 303
PspGI CCWGG 1 cut(s) 303
PspN4I GGNNCC 1 cut(s) 121
PspPI GGNCC 1 cut(s) 652
PstNI CAGNNNCTG 1 cut(s) 95
PvuI CGATCG 1 cut(s) 550
RsaI GTAC 2 cut(s) 136, 144
RsaNI GTAC 2 cut(s) 135, 143
SaqAI TTAA 3 cut(s) 164, 258, 512
SatI GCNGC 3 cut(s) 271, 485, 651
Sau3AI GATC 5 cut(s) 313, 382, 450, 547, 676
Sau96I GGNCC 1 cut(s) 652
SchI GAGTC 1 cut(s) 277
ScrFI CCNGG 1 cut(s) 305
SetI ASST 8 cut(s) 76, 97, 121, 194, 272, 520, 663, 687
SfaNI GCATC 2 cut(s) 168, 496
SmlI CTYRAG 2 cut(s) 298, 519
SmoI CTYRAG 2 cut(s) 298, 519
SsiI CCGC 4 cut(s) 113, 276, 605, 650
SspI AATATT 1 cut(s) 423
SspMI CTAG 1 cut(s) 56
StyD4I CCNGG 1 cut(s) 303
TaaI ACNGT 2 cut(s) 476, 586
TaiI ACGT 1 cut(s) 687
TaqI TCGA 6 cut(s) 8, 222, 453, 464, 471, 679
TauI GCSGC 1 cut(s) 653
TfiI GAWTC 1 cut(s) 98
Tru1I TTAA 3 cut(s) 164, 258, 512
Tru9I TTAA 3 cut(s) 164, 258, 512
TscAI CASTG 1 cut(s) 66
TseI GCWGC 2 cut(s) 270, 484
TspDTI ATGAA 4 cut(s) 9, 17, 335, 645
TspRI CASTG 1 cut(s) 66
XapI RAATTY 8 cut(s) 84, 236, 335, 369, 407, 437, 498, 527
XspI CTAG 1 cut(s) 56
Zsp2I ATGCAT 1 cut(s) 461
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.